AT5G04230

Phenylalanine ammonialyase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007050
Physical Location & Seq
Forward (+)
1160569 .. 1163957
3389 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04230.2

Sequence Viewer

Length: 2085 bp
ATGGAGTTTCGTCAACCAAACGCAACAGCATTGAGCGACCCACTTAACTGGAATGTAGCGGCGGAGGCTTTAAAAGGGAGCCACCTGGAGGAGGTGAAGAAGATGGTGAAGGATTATAGGAAAGGAACGGTGCAGCTAGGCGGAGAGACGCTGACCATCGGTCAGGTTGCGGCAGTAGCGAGTGGAGGACCGACAGTGGAGCTTTCTGAGGAGGCTCGGGGCGGTGTGAAGGCGAGTAGTGACTGGGTGATGGAGAGCATGAACCGTGATACGGACACATATGGGATCACCACTGGATTTGGTTCATCTTCTCGTAGGAGGACTGACCAAGGTGCTGCTCTTCAAAAAGAGCTTATTAGGTATTTGAACGCCGGGATATTCGCTACCGGCAACGAAGATGACGACAGGTCAAACACGCTTCCCCGGCCGGCTACTAGAGCAGCGATGCTCATCCGTGTAAACACCCTCCTCCAAGGCTACTCTGGTATACGCTTTGAGATCCTCGAAGCCATCACAACACTCCTCAACTGCAAAATTACACCGCTCCTTCCTCTCCGAGGCACCATTACCGCCTCCGGGGATCTCGTTCCGTTATCCTATATCGCTGGATTCCTCATCGGGCGCCCCAACTCCCGATCCGTGGGCCCCTCTGGCGAGATCCTCACTGCCTTGGAGGCCTTCAAGCTCGCTGGAGTATCGTCTTTTTTCGAACTCAGGCCTAAAGAAGGGCTTGCGCTCGTGAATGGGACTGCGGTGGGGTCTGCTTTAGCCTCTACGGTACTGTACGATGCCAACATTTTGGTGGTTTTCTCCGAAGTTGCTTCCGCCATGTTTGCAGAGGTTATGCAGGGGAAACCAGAGTTTACCGATCATCTTACGCATAAACTCAAGCACCATCCTGGTCAGATCGAAGCCGCCGCTATCATGGAGCATATTCTAGACGGAAGCTCTTATGTAAAAGAAGCTCTACATCTCCACAAGATTGATCCGCTTCAGAAACCTAAACAAGATCGTTACGCTCTGCGAACATCTCCGCAATGGCTTGGACCGCAGATTGAGGTGATAAGAGCAGCGACTAAGATGATCGAACGTGAGATAAACTCAGTAAACGATAACCCTTTGATCGATGTTTCAAGAAACAAAGCTATCCATGGTGGGAACTTCCAGGGGACACCAATTGGTGTCGCCATGGATAACACTCGTCTAGCACTTGCTTCTATCGGGAAGCTAATGTTCGCTCAGTTCACTGAACTCGTAAATGATTTCTACAACAACGGGTTACCCTCTAATCTATCTGGTGGTAGAAACCCTAGTCTTGATTACGGGTTAAAAGGCGCAGAAGTCGCCATGGCTTCTTATTGCTCAGAGCTTCAGTTCCTAGCAAATCCTGTGACGAACCATGTCGAAAGCGCTTCTCAACACAATCAAGATGTTAACTCTCTTGGGCTGATCTCGAGCCGAACGACAGCAGAAGCTGTGGTTATCCTCAAGCTCATGTCAACGACTTACTTGGTAGCTTTGTGCCAAGCCTTTGATCTGAGACACCTTGAAGAAATTCTCAAGAAAGCGGTTAATGAGGTTGTGAGCCACACTGCCAAAAGCGTTCTAGCAATCGAACCATTCCGCAAACACGACGATATTCTTGGAGTTGTCAACCGCGAATACGTCTTCTCCTATGTTGATGACCCAAGCAGCCTCACTAACCCTTTAATGCAGAAGCTGAGACATGTCCTCTTCGACAAAGCTTTAGCTGAACCGGAAGGCGAGACCGACACGGTTTTTCGGAAAATCGGAGCGTTCGAGGCCGAGCTGAAATTTCTCCTCCCTAAAGAAGTGGAACGAGTCAGGACAGAGTACGAGAACGGTACATTTAATGTGGCTAACCGAATCAAGAAGTGTCGATCGTATCCGCTGTACCGGTTTGTGCGGAATGAACTCGAGACGAGGTTGCTAACCGGAGAGGATGTTCGGTCACCGGGAGAGGATTTTGACAAAGTCTTCAGGGCTATATCTCAAGGAAAACTCATAGATCCTCTGTTTGAATGTCTGAAAGAGTGGAACGGTGCTCCGATTTCTATCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006082 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006979 GO:0007275 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009314 GO:0009411 GO:0009414 GO:0009415 GO:0009416 GO:0009555 GO:0009628 GO:0009636 GO:0009696 GO:0009698 GO:0009699 GO:0009719 GO:0009725 GO:0009739 GO:0009808 GO:0009819 GO:0009987 GO:0010033 GO:0010035 GO:0010224 GO:0010243 GO:0010817 GO:0014070 GO:0016054 GO:0016598 GO:0016829 GO:0016840 GO:0016841 GO:0016999 GO:0017001 GO:0017144 GO:0018958 GO:0019336 GO:0019438 GO:0019439 GO:0019538 GO:0019748 GO:0019752 GO:0019941 GO:0030163 GO:0032501 GO:0032502 GO:0032787 GO:0033993 GO:0036211 GO:0042221 GO:0042445 GO:0042447 GO:0042493 GO:0042537 GO:0042737 GO:0043170 GO:0043412 GO:0043436 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044281 GO:0044282 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045548 GO:0046244 GO:0046271 GO:0046274 GO:0046395 GO:0046677 GO:0046898 GO:0048229 GO:0048856 GO:0050896 GO:0051603 GO:0060992 GO:0065007 GO:0065008 GO:0071704 GO:0072329 GO:0080167 GO:0097305 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901615 GO:1901616 GO:1901654 GO:1901698 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00360 ko00940 ko01100 ko01110 map00360 map00940 map01100 map01110
Pfam Domains
Protein Families
Lyase_aromatic

Protein Analysis

694

Amino Acids

76.24

Weight (kDa)

6.08

Isoelectric Point (pI)

36.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lyase_aromatic PF00221 51 - 531 6.1e-149 Aromatic amino acid lyase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 560, 621
AccBSI CCGCTC 1 cut(s) 544
AccI GTMKAC 1 cut(s) 487
AccII CGCG 1 cut(s) 1659
AclWI GGATC 7 cut(s) 293, 493, 588, 630, 652, 980, 2024
AcoI YGGCCR 1 cut(s) 425
AcsI RAATTY 2 cut(s) 1554, 1814
AcuI CTGAAG 3 cut(s) 977, 1355, 1984
AcyI GRCGYC 1 cut(s) 622
AfaI GTAC 5 cut(s) 780, 785, 1856, 1867, 1916
AfeI AGCGCT 1 cut(s) 1411
AfiI CCNNNNNNNGG 7 cut(s) 88, 91, 271, 557, 576, 640, 725
AflIII ACRYGT 1 cut(s) 1726
AgeI ACCGGT 1 cut(s) 1917
AgsI TTSAA 6 cut(s) 344, 367, 682, 1134, 1550, 2042
AhdI GACNNNNNGTC 2 cut(s) 159, 406
AjnI CCWGG 3 cut(s) 84, 898, 1164
AloI GAACNNNNNNTCC 2 cut(s) 1950, 1982
Alw21I GWGCWC 1 cut(s) 2068
Alw26I GTCTC 5 cut(s) 140, 1534, 1717, 1760, 1934
AlwI GGATC 7 cut(s) 293, 493, 588, 630, 652, 980, 2024
AlwNI CAGNNNCTG 2 cut(s) 1475, 1720
Ama87I CYCGRG 3 cut(s) 216, 1453, 1937
Aor51HI AGCGCT 1 cut(s) 1411
AoxI GGCC 5 cut(s) 425, 643, 675, 716, 1803
ApaI GGGCCC 1 cut(s) 647
ApeKI GCWGC 5 cut(s) 133, 335, 440, 1070, 1692
ApoI RAATTY 2 cut(s) 1554, 1814
AsiGI ACCGGT 1 cut(s) 1917
Asp700I GAANNNNTTC 1 cut(s) 1554
AspLEI GCGC 4 cut(s) 624, 736, 1337, 1412
AspS9I GGNCC 4 cut(s) 188, 643, 644, 1046
AsuC2I CCSGG 4 cut(s) 373, 424, 577, 1977
AsuHPI GGTGA 6 cut(s) 106, 118, 259, 280, 1072, 1965
AsuII TTCGAA 1 cut(s) 708
AvaI CYCGRG 3 cut(s) 216, 1453, 1937
AvaII GGWCC 2 cut(s) 188, 1046
BaeGI GKGCMC 1 cut(s) 647
BanI GGYRCC 2 cut(s) 560, 621
BanII GRGCYC 1 cut(s) 647
BauI CACGAG 1 cut(s) 737
BbsI GAAGAC 2 cut(s) 1660, 1990
Bbv12I GWGCWC 1 cut(s) 2068
BbvI GCAGC 5 cut(s) 145, 322, 452, 1082, 1704
BccI CCATC 5 cut(s) 97, 164, 244, 518, 903
BciT130I CCWGG 3 cut(s) 86, 900, 1166
BciVI GTATCC 1 cut(s) 1917
BcnI CCSGG 4 cut(s) 373, 424, 577, 1977
BcoDI GTCTC 5 cut(s) 140, 1534, 1717, 1760, 1934
BfaI CTAG 7 cut(s) 137, 435, 938, 1205, 1311, 1379, 1607
BfoI RGCGCY 2 cut(s) 625, 1413
BfuI GTATCC 1 cut(s) 1917
BglI GCCNNNNNGGC 2 cut(s) 651, 674
BisI GCNGC 9 cut(s) 60, 134, 171, 336, 441, 915, 918, 1071, 1693
BlsI GCNGC 9 cut(s) 61, 135, 172, 337, 442, 916, 919, 1072, 1694
Bme1390I CCNGG 7 cut(s) 86, 373, 424, 577, 900, 1166, 1977
Bme18I GGWCC 2 cut(s) 188, 1046
BmeRI GACNNNNNGTC 2 cut(s) 159, 406
BmeT110I CYCGRG 3 cut(s) 216, 1453, 1937
BmgT120I GGNCC 4 cut(s) 188, 643, 644, 1046
BmiI GGNNCC 5 cut(s) 80, 562, 623, 645, 646
BmrFI CCNGG 7 cut(s) 86, 373, 424, 577, 900, 1166, 1977
BmrI ACTGGG 1 cut(s) 253
BmsI GCATC 2 cut(s) 435, 778
BmuI ACTGGG 1 cut(s) 253
BpiI GAAGAC 2 cut(s) 1660, 1990
BplI GAGNNNNNCTC 2 cut(s) 1085, 1117
BpmI CTGGAG 2 cut(s) 107, 711
Bpu14I TTCGAA 1 cut(s) 708
BpuEI CTTGAG 4 cut(s) 872, 1472, 1544, 1998
BpuMI CCSGG 4 cut(s) 373, 424, 577, 1977
Bsa29I ATCGAT 1 cut(s) 1125
BsaBI GATNNNNATC 2 cut(s) 449, 2075
BsaHI GRCGYC 1 cut(s) 622
BsaI GGTCTC 1 cut(s) 1760
BsaWI WCCGGW 3 cut(s) 1756, 1917, 1955
BsaXI ACNNNNNCTCC 4 cut(s) 1655, 1685, 1950, 1980
Bsc4I CCNNNNNNNGG 7 cut(s) 88, 91, 271, 557, 576, 640, 725
Bse118I RCCGGY 3 cut(s) 386, 427, 1917
Bse1I ACTGG 3 cut(s) 53, 248, 298
Bse3DI GCAATG 1 cut(s) 1043
Bse8I GATNNNNATC 2 cut(s) 449, 2075
BseBI CCWGG 3 cut(s) 86, 900, 1166
BseCI ATCGAT 1 cut(s) 1125
BseGI GGATG 3 cut(s) 450, 895, 1969
BseJI GATNNNNATC 2 cut(s) 449, 2075
BseLI CCNNNNNNNGG 7 cut(s) 88, 91, 271, 557, 576, 640, 725
BseMI GCAATG 1 cut(s) 1043
BseMII CTCAG 7 cut(s) 198, 727, 1116, 1253, 1377, 1529, 1712
BseNI ACTGG 3 cut(s) 53, 248, 298
BseRI GAGGAG 5 cut(s) 104, 224, 458, 512, 1811
BseSI GKGCMC 1 cut(s) 647
BseX3I CGGCCG 1 cut(s) 425
BseXI GCAGC 5 cut(s) 145, 322, 452, 1082, 1704
BsgI GTGCAG 1 cut(s) 152
Bsh1236I CGCG 1 cut(s) 1659
Bsh1285I CGRYCG 2 cut(s) 428, 1904
BshFI GGCC 5 cut(s) 427, 645, 677, 718, 1805
BshNI GGYRCC 2 cut(s) 560, 621
BshTI ACCGGT 1 cut(s) 1917
BshVI ATCGAT 1 cut(s) 1125
BsiEI CGRYCG 2 cut(s) 428, 1904
BsiHKAI GWGCWC 1 cut(s) 2068
BsiHKCI CYCGRG 3 cut(s) 216, 1453, 1937
BsiSI CCGG 9 cut(s) 372, 387, 424, 428, 576, 1757, 1918, 1956, 1976
BslFI GGGAC 2 cut(s) 760, 1183
BslI CCNNNNNNNGG 7 cut(s) 88, 91, 271, 557, 576, 640, 725
BsmAI GTCTC 5 cut(s) 140, 1534, 1717, 1760, 1934
BsmBI CGTCTC 2 cut(s) 140, 1934
BsmFI GGGAC 2 cut(s) 760, 1183
BsnI GGCC 5 cut(s) 427, 645, 677, 718, 1805
Bso31I GGTCTC 1 cut(s) 1760
BsoBI CYCGRG 3 cut(s) 216, 1453, 1937
Bsp119I TTCGAA 1 cut(s) 708
Bsp120I GGGCCC 1 cut(s) 643
Bsp1286I GDGCHC 2 cut(s) 647, 2068
Bsp19I CCATGG 3 cut(s) 1150, 1188, 1347
BspANI GGCC 5 cut(s) 427, 645, 677, 718, 1805
BspCNI CTCAG 7 cut(s) 199, 726, 1115, 1252, 1376, 1530, 1713
BspDI ATCGAT 1 cut(s) 1125
BspFNI CGCG 1 cut(s) 1659
BspLI GGNNCC 5 cut(s) 80, 562, 623, 645, 646
BspPI GGATC 7 cut(s) 293, 493, 588, 630, 652, 980, 2024
BspQI GCTCTTC 1 cut(s) 345
BspT104I TTCGAA 1 cut(s) 708
BspT107I GGYRCC 2 cut(s) 560, 621
BspTNI GGTCTC 1 cut(s) 1760
BsrBI CCGCTC 1 cut(s) 544
BsrDI GCAATG 1 cut(s) 1043
BsrFI RCCGGY 3 cut(s) 386, 427, 1917
BsrI ACTGG 3 cut(s) 53, 248, 298
BssAI RCCGGY 3 cut(s) 386, 427, 1917
BssNAI GTATAC 1 cut(s) 488
BssNI GRCGYC 1 cut(s) 622
BssSI CACGAG 1 cut(s) 737
BssT1I CCWWGG 6 cut(s) 328, 472, 669, 1150, 1188, 1347
Bst1107I GTATAC 1 cut(s) 488
Bst2BI CACGAG 1 cut(s) 737
Bst2UI CCWGG 3 cut(s) 86, 900, 1166
Bst4CI ACNGT 8 cut(s) 130, 196, 266, 778, 783, 1777, 1865, 2063
Bst6I CTCTTC 2 cut(s) 345, 1739
BstACI GRCGYC 1 cut(s) 622
BstBI TTCGAA 1 cut(s) 708
BstC8I GCNNGC 3 cut(s) 429, 687, 732
BstDEI CTNAG 8 cut(s) 207, 713, 1077, 1102, 1239, 1363, 1538, 1721
BstDSI CCRYGG 4 cut(s) 639, 1150, 1188, 1347
BstEII GGTNACC 2 cut(s) 1278, 1971
BstENI CCTNNNNNAGG 3 cut(s) 89, 555, 723
BstF5I GGATG 3 cut(s) 450, 895, 1969
BstFNI CGCG 1 cut(s) 1659
BstH2I RGCGCY 2 cut(s) 625, 1413
BstHHI GCGC 4 cut(s) 624, 736, 1337, 1412
BstMAI GTCTC 5 cut(s) 140, 1534, 1717, 1760, 1934
BstMCI CGRYCG 2 cut(s) 428, 1904
BstNI CCWGG 3 cut(s) 86, 900, 1166
BstNSI RCATGY 1 cut(s) 1730
BstPI GGTNACC 2 cut(s) 1278, 1971
BstSCI CCNGG 7 cut(s) 84, 371, 422, 575, 898, 1164, 1975
BstSLI GKGCMC 1 cut(s) 647
BstUI CGCG 1 cut(s) 1659
BstV1I GCAGC 5 cut(s) 145, 322, 452, 1082, 1704
BstV2I GAAGAC 2 cut(s) 1660, 1990
BstX2I RGATCY 4 cut(s) 498, 580, 657, 2029
BstXI CCANNNNNNTGG 2 cut(s) 48, 799
BstYI RGATCY 4 cut(s) 498, 580, 657, 2029
BstZ17I GTATAC 1 cut(s) 488
BstZI CGGCCG 1 cut(s) 425
Bsu15I ATCGAT 1 cut(s) 1125
BsuI GTATCC 1 cut(s) 1917
BsuRI GGCC 5 cut(s) 427, 645, 677, 718, 1805
BsuTUI ATCGAT 1 cut(s) 1125
BtgI CCRYGG 4 cut(s) 639, 1150, 1188, 1347
BtgZI GCGATG 1 cut(s) 458
BtsCI GGATG 3 cut(s) 450, 895, 1969
BtsI GCAGTG 2 cut(s) 663, 1590
BtsIMutI CAGTG 5 cut(s) 201, 291, 663, 1245, 1590
Cac8I GCNNGC 3 cut(s) 429, 687, 732
CaiI CAGNNNCTG 2 cut(s) 1475, 1720
CfoI GCGC 4 cut(s) 624, 736, 1337, 1412
Cfr10I RCCGGY 3 cut(s) 386, 427, 1917
Cfr13I GGNCC 4 cut(s) 188, 643, 644, 1046
ClaI ATCGAT 1 cut(s) 1125
CseI GACGC 1 cut(s) 157
Csp6I GTAC 5 cut(s) 779, 784, 1855, 1866, 1915
CspAI ACCGGT 1 cut(s) 1917
CspCI CAANNNNNGTGG 2 cut(s) 280, 315
CviAII CATG 9 cut(s) 259, 829, 925, 1151, 1189, 1348, 1400, 1495, 1727
CviQI GTAC 5 cut(s) 779, 784, 1855, 1866, 1915
DdeI CTNAG 8 cut(s) 207, 713, 1077, 1102, 1239, 1363, 1538, 1721
DinI GGCGCC 1 cut(s) 623
DraI TTTAAA 1 cut(s) 72
DriI GACNNNNNGTC 2 cut(s) 159, 406
EaeI YGGCCR 1 cut(s) 425
EagI CGGCCG 1 cut(s) 425
Eam1104I CTCTTC 2 cut(s) 345, 1739
Eam1105I GACNNNNNGTC 2 cut(s) 159, 406
EarI CTCTTC 2 cut(s) 345, 1739
EciI GGCGGA 3 cut(s) 77, 156, 814
EclXI CGGCCG 1 cut(s) 425
Eco130I CCWWGG 6 cut(s) 328, 472, 669, 1150, 1188, 1347
Eco147I AGGCCT 2 cut(s) 677, 718
Eco24I GRGCYC 1 cut(s) 647
Eco31I GGTCTC 1 cut(s) 1760
Eco47I GGWCC 2 cut(s) 188, 1046
Eco47III AGCGCT 1 cut(s) 1411
Eco52I CGGCCG 1 cut(s) 425
Eco57I CTGAAG 3 cut(s) 977, 1355, 1984
Eco88I CYCGRG 3 cut(s) 216, 1453, 1937
Eco91I GGTNACC 2 cut(s) 1278, 1971
EcoNI CCTNNNNNAGG 3 cut(s) 89, 555, 723
EcoO109I RGGNCCY 1 cut(s) 644
EcoO65I GGTNACC 2 cut(s) 1278, 1971
EcoRII CCWGG 3 cut(s) 84, 898, 1164
EcoT14I CCWWGG 6 cut(s) 328, 472, 669, 1150, 1188, 1347
EcoT38I GRGCYC 1 cut(s) 647
EgeI GGCGCC 1 cut(s) 623
EheI GGCGCC 1 cut(s) 623
ErhI CCWWGG 6 cut(s) 328, 472, 669, 1150, 1188, 1347
Esp3I CGTCTC 2 cut(s) 140, 1934
FaeI CATG 9 cut(s) 262, 832, 928, 1154, 1192, 1351, 1403, 1498, 1730
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 2 cut(s) 760, 1183
FatI CATG 9 cut(s) 258, 828, 924, 1150, 1188, 1347, 1399, 1494, 1726
FauNDI CATATG 1 cut(s) 280
FblI GTMKAC 1 cut(s) 487
Fnu4HI GCNGC 9 cut(s) 60, 134, 171, 336, 441, 915, 918, 1071, 1693
FokI GGATG 3 cut(s) 437, 882, 1976
FriOI GRGCYC 1 cut(s) 647
Fsp4HI GCNGC 9 cut(s) 60, 134, 171, 336, 441, 915, 918, 1071, 1693
FspBI CTAG 7 cut(s) 137, 435, 938, 1205, 1311, 1379, 1607
GlaI GCGC 4 cut(s) 623, 735, 1336, 1411
GluI GCNGC 9 cut(s) 60, 134, 171, 336, 441, 915, 918, 1071, 1693
GsuI CTGGAG 2 cut(s) 107, 711
HaeII RGCGCY 2 cut(s) 625, 1413
HaeIII GGCC 5 cut(s) 427, 645, 677, 718, 1805
HapII CCGG 9 cut(s) 372, 387, 424, 428, 576, 1757, 1918, 1956, 1976
HgaI GACGC 1 cut(s) 157
HhaI GCGC 4 cut(s) 624, 736, 1337, 1412
Hin1I GRCGYC 1 cut(s) 622
Hin1II CATG 9 cut(s) 262, 832, 928, 1154, 1192, 1351, 1403, 1498, 1730
Hin6I GCGC 4 cut(s) 622, 734, 1335, 1410
HinP1I GCGC 4 cut(s) 622, 734, 1335, 1410
HincII GTYRAC 4 cut(s) 14, 1435, 1500, 1654
HindII GTYRAC 4 cut(s) 14, 1435, 1500, 1654
HindIII AAGCTT 1 cut(s) 1743
HinfI GANTC 3 cut(s) 609, 1842, 1887
HpaI GTTAAC 1 cut(s) 1435
HpaII CCGG 9 cut(s) 372, 387, 424, 428, 576, 1757, 1918, 1956, 1976
HphI GGTGA 6 cut(s) 106, 118, 259, 280, 1072, 1965
Hpy166II GTNNAC 9 cut(s) 14, 460, 488, 864, 1108, 1245, 1435, 1500, 1654
Hpy8I GTNNAC 9 cut(s) 14, 460, 488, 864, 1108, 1245, 1435, 1500, 1654
Hpy99I CGWCG 1 cut(s) 1637
HpyAV CCTTC 6 cut(s) 103, 223, 557, 688, 719, 1754
HpyCH4III ACNGT 8 cut(s) 130, 196, 266, 778, 783, 1777, 1865, 2063
HpyCH4IV ACGT 2 cut(s) 1090, 1665
HpyCH4V TGCA 5 cut(s) 133, 531, 836, 847, 1714
HpyF3I CTNAG 8 cut(s) 207, 713, 1077, 1102, 1239, 1363, 1538, 1721
HpySE526I ACGT 2 cut(s) 1090, 1665
Hsp92I GRCGYC 1 cut(s) 622
Hsp92II CATG 9 cut(s) 262, 832, 928, 1154, 1192, 1351, 1403, 1498, 1730
HspAI GCGC 4 cut(s) 622, 734, 1335, 1410
KasI GGCGCC 1 cut(s) 621
KroI GCCGGC 1 cut(s) 427
KroNI GCCGGC 1 cut(s) 429
KspAI GTTAAC 1 cut(s) 1435
LguI GCTCTTC 1 cut(s) 345
LmnI GCTCC 6 cut(s) 78, 199, 549, 928, 1793, 2071
Lsp1109I GCAGC 5 cut(s) 145, 322, 452, 1082, 1704
LweI GCATC 2 cut(s) 435, 778
MaeI CTAG 7 cut(s) 137, 435, 938, 1205, 1311, 1379, 1607
MaeII ACGT 2 cut(s) 1090, 1665
MaeIII GTNAC 5 cut(s) 239, 1013, 1278, 1390, 1971
MbiI CCGCTC 1 cut(s) 544
MboII GAAGA 9 cut(s) 109, 112, 300, 332, 407, 1562, 1660, 1726, 1990
MfeI CAATTG 1 cut(s) 1176
MflI RGATCY 4 cut(s) 498, 580, 657, 2029
MhlI GDGCHC 2 cut(s) 647, 2068
MluCI AATT 4 cut(s) 534, 1176, 1554, 1814
Mly113I GGCGCC 1 cut(s) 622
MlyI GAGTC 1 cut(s) 1851
MroNI GCCGGC 1 cut(s) 427
MroXI GAANNNNTTC 1 cut(s) 1554
MseI TTAA 7 cut(s) 45, 71, 1328, 1434, 1572, 1709, 1872
MslI CAYNNNNRTG 1 cut(s) 800
MspA1I CMGCKG 1 cut(s) 1912
MspI CCGG 9 cut(s) 372, 387, 424, 428, 576, 1757, 1918, 1956, 1976
MspR9I CCNGG 7 cut(s) 86, 373, 424, 577, 900, 1166, 1977
MunI CAATTG 1 cut(s) 1176
MvaI CCWGG 3 cut(s) 86, 900, 1166
MvnI CGCG 1 cut(s) 1659
NaeI GCCGGC 1 cut(s) 429
NarI GGCGCC 1 cut(s) 622
NciI CCSGG 4 cut(s) 373, 424, 577, 1977
NcoI CCATGG 3 cut(s) 1150, 1188, 1347
NdeI CATATG 1 cut(s) 280
NgoMIV GCCGGC 1 cut(s) 427
NlaIII CATG 9 cut(s) 262, 832, 928, 1154, 1192, 1351, 1403, 1498, 1730
NlaIV GGNNCC 5 cut(s) 80, 562, 623, 645, 646
NmeAIII GCCGAG 1 cut(s) 1831
NmuCI GTSAC 3 cut(s) 239, 1390, 1971
NspI RCATGY 1 cut(s) 1730
NspV TTCGAA 1 cut(s) 708
PaeR7I CTCGAG 2 cut(s) 1453, 1937
PceI AGGCCT 2 cut(s) 677, 718
PciI ACATGT 1 cut(s) 1726
PciSI GCTCTTC 1 cut(s) 345
PcsI WCGNNNNNNNCGW 3 cut(s) 399, 1460, 1797
PdiI GCCGGC 1 cut(s) 429
PdmI GAANNNNTTC 1 cut(s) 1554
PfeI GAWTC 2 cut(s) 609, 1887
PflFI GACNNNGTC 1 cut(s) 1994
PinAI ACCGGT 1 cut(s) 1917
PkrI GCNGC 9 cut(s) 61, 135, 172, 337, 442, 916, 919, 1072, 1694
Ple19I CGATCG 1 cut(s) 1904
PleI GAGTC 1 cut(s) 1850
PluTI GGCGCC 1 cut(s) 625
PpsI GAGTC 1 cut(s) 1850
PscI ACATGT 1 cut(s) 1726
Psp6I CCWGG 3 cut(s) 84, 898, 1164
PspEI GGTNACC 2 cut(s) 1278, 1971
PspGI CCWGG 3 cut(s) 84, 898, 1164
PspN4I GGNNCC 5 cut(s) 80, 562, 623, 645, 646
PspOMI GGGCCC 1 cut(s) 643
PspPI GGNCC 4 cut(s) 188, 643, 644, 1046
PstNI CAGNNNCTG 2 cut(s) 1475, 1720
PsuI RGATCY 4 cut(s) 498, 580, 657, 2029
PsyI GACNNNGTC 1 cut(s) 1994
PvuI CGATCG 1 cut(s) 1904
RsaI GTAC 5 cut(s) 780, 785, 1856, 1867, 1916
RsaNI GTAC 5 cut(s) 779, 784, 1855, 1866, 1915
RseI CAYNNNNRTG 1 cut(s) 800
SapI GCTCTTC 1 cut(s) 345
SaqAI TTAA 7 cut(s) 45, 71, 1328, 1434, 1572, 1709, 1872
SatI GCNGC 9 cut(s) 60, 134, 171, 336, 441, 915, 918, 1071, 1693
Sau96I GGNCC 4 cut(s) 188, 643, 644, 1046
SchI GAGTC 1 cut(s) 1851
ScrFI CCNGG 7 cut(s) 86, 373, 424, 577, 900, 1166, 1977
SduI GDGCHC 2 cut(s) 647, 2068
SfaNI GCATC 2 cut(s) 435, 778
SfoI GGCGCC 1 cut(s) 623
Sfr274I CTCGAG 2 cut(s) 1453, 1937
SfuI TTCGAA 1 cut(s) 708
SinI GGWCC 2 cut(s) 188, 1046
SlaI CTCGAG 2 cut(s) 1453, 1937
SmiMI CAYNNNNRTG 1 cut(s) 800
SmlI CTYRAG 6 cut(s) 887, 1453, 1487, 1559, 1937, 2013
SmoI CTYRAG 6 cut(s) 887, 1453, 1487, 1559, 1937, 2013
Sse9I AATT 4 cut(s) 534, 1176, 1554, 1814
SseBI AGGCCT 2 cut(s) 677, 718
SspDI GGCGCC 1 cut(s) 621
SspMI CTAG 7 cut(s) 137, 435, 938, 1205, 1311, 1379, 1607
StuI AGGCCT 2 cut(s) 677, 718
StyD4I CCNGG 7 cut(s) 84, 371, 422, 575, 898, 1164, 1975
StyI CCWWGG 6 cut(s) 328, 472, 669, 1150, 1188, 1347
TaaI ACNGT 8 cut(s) 130, 196, 266, 778, 783, 1777, 1865, 2063
TaiI ACGT 2 cut(s) 1093, 1668
TaqII GACCGA 4 cut(s) 149, 205, 1784, 1959
TasI AATT 4 cut(s) 534, 1176, 1554, 1814
TauI GCSGC 4 cut(s) 62, 173, 917, 920
TfiI GAWTC 2 cut(s) 609, 1887
Tru1I TTAA 7 cut(s) 45, 71, 1328, 1434, 1572, 1709, 1872
Tru9I TTAA 7 cut(s) 45, 71, 1328, 1434, 1572, 1709, 1872
TscAI CASTG 5 cut(s) 201, 298, 670, 1252, 1597
TseFI GTSAC 3 cut(s) 239, 1390, 1971
TseI GCWGC 5 cut(s) 133, 335, 440, 1070, 1692
Tsp45I GTSAC 3 cut(s) 239, 1390, 1971
TspDTI ATGAA 3 cut(s) 275, 294, 1947
TspGWI ACGGA 5 cut(s) 287, 443, 579, 628, 957
TspRI CASTG 5 cut(s) 201, 298, 670, 1252, 1597
Tth111I GACNNNGTC 1 cut(s) 1994
VpaK11BI GGWCC 2 cut(s) 188, 1046
XagI CCTNNNNNAGG 3 cut(s) 89, 555, 723
XapI RAATTY 2 cut(s) 1554, 1814
XbaI TCTAGA 1 cut(s) 937
XceI RCATGY 1 cut(s) 1730
XcmI CCANNNNNNNNNTGG 2 cut(s) 479, 799
XhoI CTCGAG 2 cut(s) 1453, 1937
XmiI GTMKAC 1 cut(s) 487
XmnI GAANNNNTTC 1 cut(s) 1554
XspI CTAG 7 cut(s) 137, 435, 938, 1205, 1311, 1379, 1607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.