AT5G04960

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013785
Physical Location & Seq
Forward (+)
1461786 .. 1464066
2281 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G04960.1

Sequence Viewer

Length: 1695 bp
ATGTCCTCCTACGGCAGACTCGACGAGCATGAGCAAGCGAAACTTGAGGCAAGTCGGAAGACAAAGAAGAGAATTGCCATCATCGCTATATCTTCCATCGTTCTCGTCTGCATTGTCGTCGGAGCCGTCGTTGGAACCACAGCTCGCGATAACAGCAAGAAACCACCGACGGAGAATAACGGAGAGCCAATATCAGTCTCCGTGAAAGCCCTGTGCGACGTGACATTGCACAAAGAAAAATGTTTCGAGACCCTTGGATCGGCTCCAAACGCGAGCCGATCAAGCCCAGAGGAGCTTTTCAAGTACGCCGTCAAAGTCACCATCACGGAGCTCTCGAAAGTTCTCGACGGATTCTCCAACGGCGAACACATGGATAACGCCACGTCAGCAGCAATGGGGGCTTGCGTGGAGCTTATCGGGCTAGCCGTTGATCAACTCAACGAAACAATGACGTCGTCCTTGAAGAACTTTGACGATCTAAGGACGTGGCTTAGCTCCGTGGGAACGTACCAAGAAACGTGTATGGATGCGTTAGTGGAAGCGAACAAACCAAGTTTAACGACTTTCGGAGAAAACCATTTGAAAAACTCGACGGAGATGACTAGCAATGCGTTAGCGATCATAACGTGGCTAGGGAAAATCGCTGACACCGTTAAGTTTAGACGACGTCGTTTGTTGGAGACTGGTAACGCCAAAGTCGTTGTAGCTGACTTGCCGATGATGGAAGGTCGGAGACTTTTGGAGAGTGGTGATTTGAAGAAGAAAGCGACTATCGTGGTGGCTAAAGACGGGTCGGGGAAATATCGGACGATTGGTGAGGCTTTAGCGGAAGTGGAGGAGAAGAATGAGAAACCTACGATTATATATGTGAAGAAAGGAGTTTATTTAGAGAATGTTAGGGTTGAGAAGACGAAATGGAATGTTGTGATGGTTGGAGATGGACAAAGTAAGACTATCGTCTCTGCTGGACTCAACTTCATCGATGGAACCCCCACTTTCGAAACCGCCACATTTGCTGTGTTTGGAAAGGGGTTCATGGCTAGGGACATGGGCTTCATTAACACAGCTGGTCCAGCTAAGCATCAAGCCGTAGCTCTAATGGTAAGCGCAGACTTATCTGTGTTCTACAAGTGCACCATGGACGCTTTCCAAGATACAATGTACGCTCATGCACAACGTCAGTTTTACCGTGATTGTGTTATCCTCGGAACCGTTGATTTTATATTTGGAAACGCTGCGGTTGTATTCCAAAAGTGCGAGATCTTGCCTCGTCGTCCTATGAAAGGCCAGCAGAATACAATAACCGCTCAGGGCCGGAAAGATCCGAACCAAAACACAGGAATTTCTATCCACAATTGTACTATCAAGCCTTTGGATAACTTAACCGATATCCAAACATTCCTAGGCCGGCCTTGGAAGGATTTCTCTACAACGGTTATCATGAAGTCGTTCATGGACAAATTCATCAACCCGAAAGGGTGGTTACCGTGGACAGGAGATACCGCGCCAGACACGATCTTTTACGCCGAGTACTTAAATTCCGGGCCAGGAGCATCGACCAAGAACCGGGTTAAGTGGCAAGGGTTGAAGACGTCTTTAACAAAGAAGGAAGCAAATAAGTTTACGGTGAAACCTTTTATAGACGGCAATAACTGGTTGCCGGCTACCAAAGTTCCCTTCAACTCTGATTTTTGA

Protein Analysis

564

Amino Acids

62.17

Weight (kDa)

8.94

Isoelectric Point (pI)

27.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 67 - 207 2.3e-29 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 258 - 550 2.2e-146 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10710 AT5G04960
fragaria_vesca FvH4_7g04230 FvH4_7g04920 FvH4_7g04920
malus_domestica MD02G1266400.v1.1 MD07G1053300.v1.1
prunus_persica Prupe.2G058300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0331001
rosa_laevigata RLG00000029761
rosa_roxburghii Rroxscaffold_4G00319560
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113100 Rh1BG087100 Rh1CG110000 Rh1DG118800
rosa_wichuraiana Rw1G009220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 455, 670, 1595
AccBSI CCGCTC 1 cut(s) 1307
AccII CGCG 3 cut(s) 147, 272, 1505
AciI CCGC 5 cut(s) 827, 1005, 1238, 1305, 1503
AclWI GGATC 2 cut(s) 265, 1316
AcsI RAATTY 3 cut(s) 1341, 1460, 1537
AcyI GRCGYC 3 cut(s) 452, 667, 1592
AfaI GTAC 5 cut(s) 305, 509, 1163, 1360, 1532
AfiI CCNNNNNNNGG 4 cut(s) 259, 1283, 1493, 1566
AflIII ACRYGT 1 cut(s) 518
AgsI TTSAA 6 cut(s) 301, 463, 583, 757, 1588, 1681
AjiI CACGTC 3 cut(s) 220, 384, 486
AjnI CCWGG 1 cut(s) 1546
AjuI GAANNNNNNNTTGG 2 cut(s) 1661, 1693
AluBI AGCT 9 cut(s) 143, 295, 331, 412, 495, 707, 1067, 1076, 1094
AluI AGCT 9 cut(s) 143, 295, 331, 412, 495, 707, 1067, 1076, 1094
Alw21I GWGCWC 2 cut(s) 333, 1136
Alw26I GTCTC 5 cut(s) 202, 242, 674, 727, 964
Alw44I GTGCAC 1 cut(s) 1132
AlwI GGATC 2 cut(s) 265, 1316
AoxI GGCC 5 cut(s) 1285, 1312, 1405, 1409, 1544
ApaLI GTGCAC 1 cut(s) 1132
ApeKI GCWGC 2 cut(s) 389, 1235
ApoI RAATTY 3 cut(s) 1341, 1460, 1537
Asp700I GAANNNNTTC 2 cut(s) 1421, 1448
AspA2I CCTAGG 1 cut(s) 1402
AspLEI GCGC 2 cut(s) 1109, 1507
AspS9I GGNCC 3 cut(s) 1070, 1312, 1544
AsuC2I CCSGG 2 cut(s) 1543, 1568
AsuHPI GGTGA 4 cut(s) 310, 761, 827, 1639
AsuII TTCGAA 1 cut(s) 999
AsuNHI GCTAGC 1 cut(s) 421
AvaII GGWCC 1 cut(s) 1070
AvrII CCTAGG 1 cut(s) 1402
BaeGI GKGCMC 1 cut(s) 1136
BanII GRGCYC 1 cut(s) 333
BbsI GAAGAC 3 cut(s) 65, 914, 1595
Bbv12I GWGCWC 2 cut(s) 333, 1136
BbvI GCAGC 2 cut(s) 401, 1222
BccI CCATC 7 cut(s) 86, 104, 329, 715, 922, 932, 977
BceAI ACGGC 7 cut(s) 28, 110, 293, 376, 410, 1073, 1660
BcgI CGANNNNNNTGC 2 cut(s) 100, 134
BciT130I CCWGG 1 cut(s) 1548
BclI TGATCA 1 cut(s) 430
BcnI CCSGG 2 cut(s) 1543, 1568
BcoDI GTCTC 5 cut(s) 202, 242, 674, 727, 964
BfaI CTAG 5 cut(s) 422, 603, 632, 1041, 1403
BglII AGATCT 1 cut(s) 1260
BisI GCNGC 2 cut(s) 390, 1236
BlnI CCTAGG 1 cut(s) 1402
BlpI GCTNAGC 2 cut(s) 491, 1077
BlsI GCNGC 2 cut(s) 391, 1237
BmcAI AGTACT 1 cut(s) 1532
Bme1390I CCNGG 3 cut(s) 1543, 1548, 1568
Bme18I GGWCC 1 cut(s) 1070
BmgBI CACGTC 3 cut(s) 220, 384, 486
BmgT120I GGNCC 3 cut(s) 1070, 1312, 1544
BmiI GGNNCC 5 cut(s) 124, 136, 264, 988, 1210
BmrFI CCNGG 3 cut(s) 1543, 1548, 1568
BmsI GCATC 3 cut(s) 517, 1090, 1562
BmtI GCTAGC 1 cut(s) 425
BoxI GACNNNNGTC 1 cut(s) 956
BpiI GAAGAC 3 cut(s) 65, 914, 1595
Bpu10I CCTNAGC 1 cut(s) 1308
Bpu1102I GCTNAGC 2 cut(s) 491, 1077
Bpu14I TTCGAA 1 cut(s) 999
BpuEI CTTGAG 1 cut(s) 65
BpuMI CCSGG 2 cut(s) 1543, 1568
Bsa29I ATCGAT 1 cut(s) 981
BsaHI GRCGYC 3 cut(s) 452, 667, 1592
BsaI GGTCTC 1 cut(s) 242
BsaJI CCNNGG 7 cut(s) 253, 498, 1137, 1204, 1402, 1412, 1487
BsaXI ACNNNNNCTCC 4 cut(s) 114, 144, 338, 368
Bsc4I CCNNNNNNNGG 4 cut(s) 259, 1283, 1493, 1566
Bse118I RCCGGY 2 cut(s) 1407, 1660
Bse1I ACTGG 2 cut(s) 688, 1658
Bse3DI GCAATG 3 cut(s) 224, 399, 613
BseBI CCWGG 1 cut(s) 1548
BseCI ATCGAT 1 cut(s) 981
BseDI CCNNGG 7 cut(s) 253, 498, 1137, 1204, 1402, 1412, 1487
BseGI GGATG 1 cut(s) 532
BseLI CCNNNNNNNGG 4 cut(s) 259, 1283, 1493, 1566
BseMI GCAATG 3 cut(s) 224, 399, 613
BseMII CTCAG 1 cut(s) 1322
BseNI ACTGG 2 cut(s) 688, 1658
BseRI GAGGAG 2 cut(s) 305, 851
BseSI GKGCMC 1 cut(s) 1136
BseXI GCAGC 2 cut(s) 401, 1222
Bsh1236I CGCG 3 cut(s) 147, 272, 1505
BshFI GGCC 5 cut(s) 1287, 1314, 1407, 1411, 1546
BshVI ATCGAT 1 cut(s) 981
BsiHKAI GWGCWC 2 cut(s) 333, 1136
BsiSI CCGG 5 cut(s) 1315, 1408, 1542, 1567, 1661
BslFI GGGAC 1 cut(s) 1058
BslI CCNNNNNNNGG 4 cut(s) 259, 1283, 1493, 1566
BsmAI GTCTC 5 cut(s) 202, 242, 674, 727, 964
BsmBI CGTCTC 1 cut(s) 964
BsmFI GGGAC 1 cut(s) 1058
BsnI GGCC 5 cut(s) 1287, 1314, 1407, 1411, 1546
Bso31I GGTCTC 1 cut(s) 242
Bsp119I TTCGAA 1 cut(s) 999
Bsp1286I GDGCHC 2 cut(s) 333, 1136
Bsp143I GATC 8 cut(s) 257, 278, 430, 475, 618, 1260, 1321, 1515
Bsp1720I GCTNAGC 2 cut(s) 491, 1077
Bsp19I CCATGG 1 cut(s) 1137
Bsp68I TCGCGA 1 cut(s) 147
BspACI CCGC 5 cut(s) 827, 1005, 1238, 1305, 1503
BspANI GGCC 5 cut(s) 1287, 1314, 1407, 1411, 1546
BspCNI CTCAG 1 cut(s) 1321
BspDI ATCGAT 1 cut(s) 981
BspFNI CGCG 3 cut(s) 147, 272, 1505
BspHI TCATGA 1 cut(s) 1440
BspLI GGNNCC 5 cut(s) 124, 136, 264, 988, 1210
BspOI GCTAGC 1 cut(s) 425
BspPI GGATC 2 cut(s) 265, 1316
BspT104I TTCGAA 1 cut(s) 999
BspTNI GGTCTC 1 cut(s) 242
BsrBI CCGCTC 1 cut(s) 1307
BsrDI GCAATG 3 cut(s) 224, 399, 613
BsrFI RCCGGY 2 cut(s) 1407, 1660
BsrI ACTGG 2 cut(s) 688, 1658
BssAI RCCGGY 2 cut(s) 1407, 1660
BssECI CCNNGG 7 cut(s) 253, 498, 1137, 1204, 1402, 1412, 1487
BssMI GATC 8 cut(s) 257, 278, 430, 475, 618, 1260, 1321, 1515
BssNI GRCGYC 3 cut(s) 452, 667, 1592
BssT1I CCWWGG 4 cut(s) 253, 1137, 1402, 1412
Bst2UI CCWGG 1 cut(s) 1548
Bst4CI ACNGT 6 cut(s) 652, 1190, 1213, 1435, 1488, 1627
Bst6I CTCTTC 1 cut(s) 62
BstACI GRCGYC 3 cut(s) 452, 667, 1592
BstBI TTCGAA 1 cut(s) 999
BstC8I GCNNGC 8 cut(s) 36, 145, 274, 403, 423, 1289, 1409, 1662
BstDEI CTNAG 4 cut(s) 479, 491, 1077, 1308
BstDSI CCRYGG 3 cut(s) 498, 1137, 1487
BstEII GGTNACC 1 cut(s) 1482
BstENI CCTNNNNNAGG 1 cut(s) 1281
BstF5I GGATG 1 cut(s) 532
BstFNI CGCG 3 cut(s) 147, 272, 1505
BstHHI GCGC 2 cut(s) 1109, 1507
BstKTI GATC 8 cut(s) 260, 281, 433, 478, 621, 1263, 1324, 1518
BstMAI GTCTC 5 cut(s) 202, 242, 674, 727, 964
BstMBI GATC 8 cut(s) 257, 278, 430, 475, 618, 1260, 1321, 1515
BstMWI GCNNNNNNNGC 9 cut(s) 83, 153, 269, 282, 386, 398, 418, 1013, 1073
BstNI CCWGG 1 cut(s) 1548
BstPAI GACNNNNGTC 1 cut(s) 956
BstPI GGTNACC 1 cut(s) 1482
BstSCI CCNGG 3 cut(s) 1541, 1546, 1566
BstSLI GKGCMC 1 cut(s) 1136
BstUI CGCG 3 cut(s) 147, 272, 1505
BstV1I GCAGC 2 cut(s) 401, 1222
BstV2I GAAGAC 3 cut(s) 65, 914, 1595
BstX2I RGATCY 2 cut(s) 1260, 1321
BstYI RGATCY 2 cut(s) 1260, 1321
Bsu15I ATCGAT 1 cut(s) 981
BsuRI GGCC 5 cut(s) 1287, 1314, 1407, 1411, 1546
BsuTUI ATCGAT 1 cut(s) 981
BtgI CCRYGG 3 cut(s) 498, 1137, 1487
BtgZI GCGATG 1 cut(s) 67
BtrI CACGTC 3 cut(s) 220, 384, 486
BtsCI GGATG 1 cut(s) 532
BtuMI TCGCGA 1 cut(s) 147
Cac8I GCNNGC 8 cut(s) 36, 145, 274, 403, 423, 1289, 1409, 1662
CciI TCATGA 1 cut(s) 1440
CfoI GCGC 2 cut(s) 1109, 1507
Cfr10I RCCGGY 2 cut(s) 1407, 1660
Cfr13I GGNCC 3 cut(s) 1070, 1312, 1544
ClaI ATCGAT 1 cut(s) 981
CseI GACGC 1 cut(s) 1151
Csp6I GTAC 5 cut(s) 304, 508, 1162, 1359, 1531
CviAII CATG 8 cut(s) 29, 370, 1036, 1048, 1138, 1169, 1441, 1453
CviQI GTAC 5 cut(s) 304, 508, 1162, 1359, 1531
DdeI CTNAG 4 cut(s) 479, 491, 1077, 1308
DpnI GATC 8 cut(s) 259, 280, 432, 477, 620, 1262, 1323, 1517
DpnII GATC 8 cut(s) 257, 278, 430, 475, 618, 1260, 1321, 1515
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 331
Eco130I CCWWGG 4 cut(s) 253, 1137, 1402, 1412
Eco24I GRGCYC 1 cut(s) 333
Eco31I GGTCTC 1 cut(s) 242
Eco32I GATATC 1 cut(s) 1390
Eco47I GGWCC 1 cut(s) 1070
Eco53kI GAGCTC 1 cut(s) 331
Eco91I GGTNACC 1 cut(s) 1482
EcoICRI GAGCTC 1 cut(s) 331
EcoNI CCTNNNNNAGG 1 cut(s) 1281
EcoO65I GGTNACC 1 cut(s) 1482
EcoRII CCWGG 1 cut(s) 1546
EcoRV GATATC 1 cut(s) 1390
EcoT14I CCWWGG 4 cut(s) 253, 1137, 1402, 1412
EcoT38I GRGCYC 1 cut(s) 333
ErhI CCWWGG 4 cut(s) 253, 1137, 1402, 1412
Esp3I CGTCTC 1 cut(s) 964
FaeI CATG 8 cut(s) 32, 373, 1039, 1051, 1141, 1172, 1444, 1456
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 1 cut(s) 1058
FatI CATG 8 cut(s) 28, 369, 1035, 1047, 1137, 1168, 1440, 1452
FbaI TGATCA 1 cut(s) 430
Fnu4HI GCNGC 2 cut(s) 390, 1236
FokI GGATG 1 cut(s) 539
FriOI GRGCYC 1 cut(s) 333
FseI GGCCGGCC 1 cut(s) 1411
Fsp4HI GCNGC 2 cut(s) 390, 1236
FspBI CTAG 5 cut(s) 422, 603, 632, 1041, 1403
GlaI GCGC 2 cut(s) 1108, 1506
GluI GCNGC 2 cut(s) 390, 1236
HaeIII GGCC 5 cut(s) 1287, 1314, 1407, 1411, 1546
HapII CCGG 5 cut(s) 1315, 1408, 1542, 1567, 1661
HgaI GACGC 1 cut(s) 1151
HhaI GCGC 2 cut(s) 1109, 1507
Hin1I GRCGYC 3 cut(s) 452, 667, 1592
Hin1II CATG 8 cut(s) 32, 373, 1039, 1051, 1141, 1172, 1444, 1456
Hin6I GCGC 2 cut(s) 1107, 1505
HinP1I GCGC 2 cut(s) 1107, 1505
HinfI GANTC 3 cut(s) 18, 351, 969
HpaII CCGG 5 cut(s) 1315, 1408, 1542, 1567, 1661
HphI GGTGA 4 cut(s) 310, 761, 827, 1639
Hpy166II GTNNAC 3 cut(s) 1134, 1491, 1623
Hpy188I TCNGA 8 cut(s) 57, 122, 569, 732, 807, 1208, 1326, 1687
Hpy188III TCNNGA 5 cut(s) 146, 247, 334, 344, 1441
Hpy8I GTNNAC 3 cut(s) 1134, 1491, 1623
HpyAV CCTTC 4 cut(s) 719, 1411, 1600, 1687
HpyCH4III ACNGT 6 cut(s) 652, 1190, 1213, 1435, 1488, 1627
HpyCH4V TGCA 4 cut(s) 111, 229, 1134, 1172
HpyF10VI GCNNNNNNNGC 9 cut(s) 83, 153, 269, 282, 386, 398, 418, 1013, 1073
HpyF3I CTNAG 4 cut(s) 479, 491, 1077, 1308
Hsp92I GRCGYC 3 cut(s) 452, 667, 1592
Hsp92II CATG 8 cut(s) 32, 373, 1039, 1051, 1141, 1172, 1444, 1456
HspAI GCGC 2 cut(s) 1107, 1505
KroI GCCGGC 2 cut(s) 1407, 1660
KroNI GCCGGC 2 cut(s) 1409, 1662
Ksp22I TGATCA 1 cut(s) 430
Kzo9I GATC 8 cut(s) 257, 278, 430, 475, 618, 1260, 1321, 1515
LmnI GCTCC 7 cut(s) 122, 268, 292, 328, 409, 500, 1550
Lsp1109I GCAGC 2 cut(s) 401, 1222
LweI GCATC 3 cut(s) 517, 1090, 1562
MaeI CTAG 5 cut(s) 422, 603, 632, 1041, 1403
MaeIII GTNAC 4 cut(s) 220, 316, 686, 1482
MalI GATC 8 cut(s) 259, 280, 432, 477, 620, 1262, 1323, 1517
MbiI CCGCTC 1 cut(s) 1307
MboI GATC 8 cut(s) 257, 278, 430, 475, 618, 1260, 1321, 1515
MfeI CAATTG 1 cut(s) 1354
MflI RGATCY 2 cut(s) 1260, 1321
MhlI GDGCHC 2 cut(s) 333, 1136
MluCI AATT 5 cut(s) 72, 1341, 1354, 1460, 1537
MlyI GAGTC 2 cut(s) 12, 963
MmeI TCCRAC 7 cut(s) 35, 100, 112, 381, 657, 710, 913
MnlI CCTC 7 cut(s) 16, 40, 283, 811, 829, 1214, 1278
MroNI GCCGGC 2 cut(s) 1407, 1660
MroXI GAANNNNTTC 2 cut(s) 1421, 1448
MseI TTAA 7 cut(s) 557, 654, 1059, 1382, 1535, 1572, 1598
MspA1I CMGCKG 1 cut(s) 1067
MspI CCGG 5 cut(s) 1315, 1408, 1542, 1567, 1661
MspR9I CCNGG 3 cut(s) 1543, 1548, 1568
MunI CAATTG 1 cut(s) 1354
MvaI CCWGG 1 cut(s) 1548
MvnI CGCG 3 cut(s) 147, 272, 1505
MwoI GCNNNNNNNGC 9 cut(s) 83, 153, 269, 282, 386, 398, 418, 1013, 1073
NaeI GCCGGC 2 cut(s) 1409, 1662
NciI CCSGG 2 cut(s) 1543, 1568
NcoI CCATGG 1 cut(s) 1137
NdeII GATC 8 cut(s) 257, 278, 430, 475, 618, 1260, 1321, 1515
NgoMIV GCCGGC 2 cut(s) 1407, 1660
NheI GCTAGC 1 cut(s) 421
NlaIII CATG 8 cut(s) 32, 373, 1039, 1051, 1141, 1172, 1444, 1456
NlaIV GGNNCC 5 cut(s) 124, 136, 264, 988, 1210
NmeAIII GCCGAG 1 cut(s) 1552
NmuCI GTSAC 2 cut(s) 220, 316
NruI TCGCGA 1 cut(s) 147
NspV TTCGAA 1 cut(s) 999
PagI TCATGA 1 cut(s) 1440
PcsI WCGNNNNNNNCGW 7 cut(s) 18, 123, 126, 332, 423, 648, 696
PdiI GCCGGC 2 cut(s) 1409, 1662
PdmI GAANNNNTTC 2 cut(s) 1421, 1448
PfeI GAWTC 1 cut(s) 351
PflFI GACNNNGTC 2 cut(s) 454, 666
PkrI GCNGC 2 cut(s) 391, 1237
PleI GAGTC 2 cut(s) 12, 963
PpsI GAGTC 2 cut(s) 12, 963
PshAI GACNNNNGTC 1 cut(s) 956
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 1546
PspEI GGTNACC 1 cut(s) 1482
PspGI CCWGG 1 cut(s) 1546
PspN4I GGNNCC 5 cut(s) 124, 136, 264, 988, 1210
PspPI GGNCC 3 cut(s) 1070, 1312, 1544
PsuI RGATCY 2 cut(s) 1260, 1321
PsyI GACNNNGTC 2 cut(s) 454, 666
PvuII CAGCTG 1 cut(s) 1067
RigI GGCCGGCC 1 cut(s) 1411
RruI TCGCGA 1 cut(s) 147
RsaI GTAC 5 cut(s) 305, 509, 1163, 1360, 1532
RsaNI GTAC 5 cut(s) 304, 508, 1162, 1359, 1531
SacI GAGCTC 1 cut(s) 333
SaqAI TTAA 7 cut(s) 557, 654, 1059, 1382, 1535, 1572, 1598
SatI GCNGC 2 cut(s) 390, 1236
Sau3AI GATC 8 cut(s) 257, 278, 430, 475, 618, 1260, 1321, 1515
Sau96I GGNCC 3 cut(s) 1070, 1312, 1544
ScaI AGTACT 1 cut(s) 1532
SchI GAGTC 2 cut(s) 12, 963
ScrFI CCNGG 3 cut(s) 1543, 1548, 1568
SduI GDGCHC 2 cut(s) 333, 1136
SfaNI GCATC 3 cut(s) 517, 1090, 1562
SfuI TTCGAA 1 cut(s) 999
SinI GGWCC 1 cut(s) 1070
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 5 cut(s) 72, 1341, 1354, 1460, 1537
SsiI CCGC 5 cut(s) 827, 1005, 1238, 1305, 1503
SspMI CTAG 5 cut(s) 422, 603, 632, 1041, 1403
SstI GAGCTC 1 cut(s) 333
StyD4I CCNGG 3 cut(s) 1541, 1546, 1566
StyI CCWWGG 4 cut(s) 253, 1137, 1402, 1412
TaaI ACNGT 6 cut(s) 652, 1190, 1213, 1435, 1488, 1627
TaqI TCGA 8 cut(s) 21, 246, 335, 345, 590, 981, 999, 1556
TasI AATT 5 cut(s) 72, 1341, 1354, 1460, 1537
TatI WGTACW 2 cut(s) 1358, 1530
TfiI GAWTC 1 cut(s) 351
Tru1I TTAA 7 cut(s) 557, 654, 1059, 1382, 1535, 1572, 1598
Tru9I TTAA 7 cut(s) 557, 654, 1059, 1382, 1535, 1572, 1598
TseFI GTSAC 2 cut(s) 220, 316
TseI GCWGC 2 cut(s) 389, 1235
Tsp45I GTSAC 2 cut(s) 220, 316
TspDTI ATGAA 7 cut(s) 967, 1024, 1045, 1295, 1441, 1453, 1457
TspGWI ACGGA 7 cut(s) 185, 190, 195, 341, 363, 487, 608
Tth111I GACNNNGTC 2 cut(s) 454, 666
VneI GTGCAC 1 cut(s) 1132
VpaK11BI GGWCC 1 cut(s) 1070
XagI CCTNNNNNAGG 1 cut(s) 1281
XapI RAATTY 3 cut(s) 1341, 1460, 1537
XmaJI CCTAGG 1 cut(s) 1402
XmnI GAANNNNTTC 2 cut(s) 1421, 1448
XspI CTAG 5 cut(s) 422, 603, 632, 1041, 1403
ZraI GACGTC 3 cut(s) 453, 668, 1593
ZrmI AGTACT 1 cut(s) 1532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.