FvH4_7g04230

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013785
Physical Location & Seq
Reverse (-)
4833793 .. 4835761
1969 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04230.t1

Sequence Viewer

Length: 1644 bp
ATGTCTTCCTTCAAAGCTTATGGCAAAGTCAGTGAATCCGACCAAGCCAAGCTCGACGCTCGTCGCAAGACTTGCAAGAGAGTCGCCATCATATCCATTTCCTCCATAGTTCTTGTCTGTGTCATCGTCGCCTCCGTGGTTGGAACCGCTACTCATAACTCCAAAAAAGGTGACGACAGTGGCAAAGGGACACTTTCCGCATCCGTCAAAGCCGTTTGTGACGTGACACTGTACCAGGACTCGTGTTATTCTAGTCTTGTGGCGATTGCGGAACTATCCAAAGCCGCCGCTCAAATTTCGGATCAAGGGCTCTTCAATGGTACTGTCACGGACAAATTGTCAGTGGAAGCTTTGAAAAACTGCGGAGAGCTTCTTGGTCTTGCCTTGGATCATCTCAACGAGTCGTTGTCTGGGGATGGTTCCTTGCTTGAAGTGGTTGAAGATCTCAAAACATGGTTGAGCTCTGCAGGAACTTACCAGCAGTCATGTGTAGATGGGTTTGATGATGCAAAAGGAGAGCTCAAGGATACAATTGAAAAGTATCTCAAGACTTCTACTGAGCTAACTAGTAACAGCCTTGCCATTGTCTCTTGGATATCGAAAGTAGCTAACTCTGTGAAGCTAAGGCGGTTGCTGAGTCACGACGGTACCGCGACTAGCCATGGCATCCCCGAGTGGCTGCACCAGACAGACCGGAGGCTAATTGAAAGTTCTGATTTGAGGAAGAAGGCCAACATTGTTGTGGCGAAAGATGGGAGTGGTAAGTACAAGACAATAGGTGATGCACTCAAGGCTGTGCCGGACAAAAGCAAGAAGAGAACAGTCATCTACGTGAAGAAGGGGGTTTATTTCGAGAATGTTCGAGTCGAGAAAACCAAGTGGAATGTTGTAGTGGTGGGCGATGGAATGACTGCAACGATTGTGTCTGCCAGCCTCAACGTTGTGGACTTTTCCAGTGCAACATTTGCCGTATTTGGGAAGGGATTCGTTGCTAGAGACATTGGATTTCGTAACACAGCTGGAGCAGCCAAGCACCAGGCGGTGGCACTAATGTCGAGTGCTGACCAGACGGTGTTCTACAGATGCCGCATCGATGCGTTCCAGGACTCGCTCTACGTTCATGCCAATCGCCAATTCTACCGAGAGTGCGACATCTACGGAACAGTTGATTTCATTTTTGGAAACTCAGCAGTAGTCCTCCAGAACTGTAAGGTGTTCCCCAGGGTACCAATGACTGGCCAGCAGAACACAATCACGGCACAAGGCAAAATCGACCCTAACCAAAACACAGGAATCTCATTTCAAAACTGCACCATTTCGCCTTTCGGGGACTTGTCTTCGGTCAAGACTTACCTCGGGAGGCCTTGGAAGAACTACTCCACAACTGTGTACATGCAGACATTCATGGGAAGCTTGATTCACCCAGATGGATGGTTGCCATGGGTAGGCACAACGGCACCTGACACCATTTTCTACTCCGAGTATCAGAATTTCGGACCTGGTTCTGTGACAAAGAATAGAGTTAAGTGGAAGGGCTTGAAAACAATCACCAGCAAAGTGGCCAGCAAATTCACCGTGCACTCATTTCTTCTGGGATCCAAGTGGATCTCCGATGCGAGTGTTCCATACAAGTCCAAACTATGA

Protein Analysis

548

Amino Acids

59.61

Weight (kDa)

9.2

Isoelectric Point (pI)

25.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 68 - 195 2.8e-22 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 245 - 532 1.4e-133 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10710 AT5G04960
fragaria_vesca FvH4_7g04230 FvH4_7g04920 FvH4_7g04920
malus_domestica MD02G1266400.v1.1 MD07G1053300.v1.1
prunus_persica Prupe.2G058300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0331001
rosa_laevigata RLG00000029761
rosa_roxburghii Rroxscaffold_4G00319560
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113100 Rh1BG087100 Rh1CG110000 Rh1DG118800
rosa_wichuraiana Rw1G009220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 647, 1225
AccB1I GGYRCC 3 cut(s) 647, 1225, 1456
AccB7I CCANNNNNTGG 2 cut(s) 1042, 1235
AccBSI CCGCTC 1 cut(s) 290
AccII CGCG 1 cut(s) 653
AclI AACGTT 1 cut(s) 939
AclWI GGATC 5 cut(s) 309, 396, 1590, 1603, 1613
AcoI YGGCCR 2 cut(s) 1237, 1560
AcsI RAATTY 3 cut(s) 294, 1489, 1568
AfaI GTAC 6 cut(s) 233, 322, 649, 767, 1227, 1391
AfiI CCNNNNNNNGG 4 cut(s) 975, 1042, 1235, 1445
AgsI TTSAA 9 cut(s) 13, 316, 355, 431, 440, 536, 707, 1304, 1540
AhdI GACNNNNNGTC 1 cut(s) 337
AhlI ACTAGT 1 cut(s) 566
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 5 cut(s) 234, 1035, 1101, 1220, 1498
AjuI GAANNNNNNNTTGG 2 cut(s) 1118, 1150
AleI CACNNNNGTG 1 cut(s) 1385
Alw21I GWGCWC 3 cut(s) 464, 522, 1581
Alw26I GTCTC 2 cut(s) 592, 990
Alw44I GTGCAC 1 cut(s) 1577
AlwI GGATC 5 cut(s) 309, 396, 1590, 1603, 1613
Ama87I CYCGRG 2 cut(s) 671, 1355
AoxI GGCC 4 cut(s) 729, 1237, 1361, 1560
ApaLI GTGCAC 1 cut(s) 1577
ApeKI GCWGC 2 cut(s) 679, 1025
ApoI RAATTY 3 cut(s) 294, 1489, 1568
Asp700I GAANNNNTTC 1 cut(s) 983
Asp718I GGTACC 2 cut(s) 647, 1225
AspS9I GGNCC 1 cut(s) 1496
AsuHPI GGTGA 5 cut(s) 182, 791, 1412, 1540, 1564
AvaI CYCGRG 2 cut(s) 671, 1355
AvaII GGWCC 1 cut(s) 1496
BaeGI GKGCMC 1 cut(s) 1581
BaeI ACNNNNGTAYC 2 cut(s) 631, 664
BalI TGGCCA 2 cut(s) 1239, 1562
BamHI GGATCC 1 cut(s) 1595
BanI GGYRCC 3 cut(s) 647, 1225, 1456
BanII GRGCYC 3 cut(s) 312, 464, 522
BauI CACGAG 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 1329
Bbv12I GWGCWC 3 cut(s) 464, 522, 1581
BbvI GCAGC 2 cut(s) 666, 1037
BccI CCATC 7 cut(s) 95, 410, 488, 746, 896, 1421, 1425
BceAI ACGGC 4 cut(s) 197, 953, 1272, 1470
BcgI CGANNNNNNTGC 4 cut(s) 64, 98, 1035, 1069
BciT130I CCWGG 5 cut(s) 236, 1037, 1103, 1222, 1500
BciVI GTATCC 1 cut(s) 520
BcoDI GTCTC 2 cut(s) 592, 990
BcuI ACTAGT 1 cut(s) 566
BfaI CTAG 4 cut(s) 252, 567, 657, 993
BfmI CTRYAG 2 cut(s) 465, 1078
BfuI GTATCC 1 cut(s) 520
BglII AGATCT 1 cut(s) 442
BisI GCNGC 5 cut(s) 285, 288, 680, 1026, 1087
BlsI GCNGC 5 cut(s) 286, 289, 681, 1027, 1088
Bme1390I CCNGG 5 cut(s) 236, 1037, 1103, 1222, 1500
Bme18I GGWCC 1 cut(s) 1496
BmeRI GACNNNNNGTC 1 cut(s) 337
BmeT110I CYCGRG 2 cut(s) 671, 1355
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 1 cut(s) 1496
BmiI GGNNCC 6 cut(s) 145, 421, 649, 1227, 1458, 1597
BmrFI CCNGG 5 cut(s) 236, 1037, 1103, 1222, 1500
BmsI GCATC 8 cut(s) 209, 496, 675, 772, 1073, 1084, 1098, 1603
BoxI GACNNNNGTC 1 cut(s) 60
BpiI GAAGAC 1 cut(s) 1329
BpmI CTGGAG 2 cut(s) 1041, 1184
Bpu10I CCTNAGC 1 cut(s) 623
BpuEI CTTGAG 3 cut(s) 506, 530, 773
Bsa29I ATCGAT 1 cut(s) 1092
BsaAI YACGTR 1 cut(s) 832
BsaJI CCNNGG 8 cut(s) 135, 384, 661, 1220, 1221, 1354, 1364, 1439
BsaWI WCCGGW 1 cut(s) 693
Bsc4I CCNNNNNNNGG 4 cut(s) 975, 1042, 1235, 1445
Bse1I ACTGG 2 cut(s) 954, 1240
BseBI CCWGG 5 cut(s) 236, 1037, 1103, 1222, 1500
BseCI ATCGAT 1 cut(s) 1092
BseDI CCNNGG 8 cut(s) 135, 384, 661, 1220, 1221, 1354, 1364, 1439
BseGI GGATG 4 cut(s) 200, 421, 666, 1436
BseLI CCNNNNNNNGG 4 cut(s) 975, 1042, 1235, 1445
BseMII CTCAG 3 cut(s) 549, 626, 1200
BseNI ACTGG 2 cut(s) 954, 1240
BseSI GKGCMC 1 cut(s) 1581
BseXI GCAGC 2 cut(s) 666, 1037
BsgI GTGCAG 2 cut(s) 665, 1294
Bsh1236I CGCG 1 cut(s) 653
BshFI GGCC 4 cut(s) 731, 1239, 1363, 1562
BshNI GGYRCC 3 cut(s) 647, 1225, 1456
BshVI ATCGAT 1 cut(s) 1092
BsiHKAI GWGCWC 3 cut(s) 464, 522, 1581
BsiHKCI CYCGRG 2 cut(s) 671, 1355
BsiSI CCGG 2 cut(s) 694, 800
BslFI GGGAC 2 cut(s) 202, 1343
BslI CCNNNNNNNGG 4 cut(s) 975, 1042, 1235, 1445
BsmAI GTCTC 2 cut(s) 592, 990
BsmFI GGGAC 2 cut(s) 202, 1343
BsnI GGCC 4 cut(s) 731, 1239, 1363, 1562
BsoBI CYCGRG 2 cut(s) 671, 1355
Bsp1286I GDGCHC 4 cut(s) 312, 464, 522, 1581
Bsp1407I TGTACA 1 cut(s) 1389
Bsp143I GATC 5 cut(s) 301, 388, 442, 1595, 1605
Bsp19I CCATGG 2 cut(s) 661, 1439
BspANI GGCC 4 cut(s) 731, 1239, 1363, 1562
BspCNI CTCAG 3 cut(s) 550, 627, 1199
BspDI ATCGAT 1 cut(s) 1092
BspFNI CGCG 1 cut(s) 653
BspLI GGNNCC 6 cut(s) 145, 421, 649, 1227, 1458, 1597
BspMAI CTGCAG 1 cut(s) 469
BspPI GGATC 5 cut(s) 309, 396, 1590, 1603, 1613
BspQI GCTCTTC 1 cut(s) 317
BspT107I GGYRCC 3 cut(s) 647, 1225, 1456
BsrBI CCGCTC 1 cut(s) 290
BsrGI TGTACA 1 cut(s) 1389
BsrI ACTGG 2 cut(s) 954, 1240
BssECI CCNNGG 8 cut(s) 135, 384, 661, 1220, 1221, 1354, 1364, 1439
BssMI GATC 5 cut(s) 301, 388, 442, 1595, 1605
BssSI CACGAG 1 cut(s) 241
BssT1I CCWWGG 4 cut(s) 384, 661, 1364, 1439
Bst2BI CACGAG 1 cut(s) 241
Bst2UI CCWGG 5 cut(s) 236, 1037, 1103, 1222, 1500
Bst6I CTCTTC 2 cut(s) 317, 809
BstAPI GCANNNNNTGC 2 cut(s) 72, 965
BstAUI TGTACA 1 cut(s) 1389
BstBAI YACGTR 1 cut(s) 832
BstC8I GCNNGC 3 cut(s) 931, 1241, 1564
BstDEI CTNAG 4 cut(s) 558, 623, 635, 1186
BstDSI CCRYGG 3 cut(s) 135, 661, 1439
BstF5I GGATG 4 cut(s) 200, 421, 666, 1436
BstFNI CGCG 1 cut(s) 653
BstKTI GATC 5 cut(s) 304, 391, 445, 1598, 1608
BstMAI GTCTC 2 cut(s) 592, 990
BstMBI GATC 5 cut(s) 301, 388, 442, 1595, 1605
BstMWI GCNNNNNNNGC 4 cut(s) 72, 791, 965, 1025
BstNI CCWGG 5 cut(s) 236, 1037, 1103, 1222, 1500
BstNSI RCATGY 1 cut(s) 1396
BstPAI GACNNNNGTC 1 cut(s) 60
BstSCI CCNGG 5 cut(s) 234, 1035, 1101, 1220, 1498
BstSFI CTRYAG 2 cut(s) 465, 1078
BstSLI GKGCMC 1 cut(s) 1581
BstUI CGCG 1 cut(s) 653
BstV1I GCAGC 2 cut(s) 666, 1037
BstV2I GAAGAC 1 cut(s) 1329
BstX2I RGATCY 3 cut(s) 442, 1595, 1605
BstXI CCANNNNNNTGG 2 cut(s) 1431, 1558
BstYI RGATCY 3 cut(s) 442, 1595, 1605
Bsu15I ATCGAT 1 cut(s) 1092
BsuI GTATCC 1 cut(s) 520
BsuRI GGCC 4 cut(s) 731, 1239, 1363, 1562
BsuTUI ATCGAT 1 cut(s) 1092
BtgI CCRYGG 3 cut(s) 135, 661, 1439
BtgZI GCGATG 1 cut(s) 915
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 4 cut(s) 200, 421, 666, 1436
BtsIMutI CAGTG 5 cut(s) 37, 184, 227, 348, 961
Cac8I GCNNGC 3 cut(s) 931, 1241, 1564
Cfr13I GGNCC 1 cut(s) 1496
ClaI ATCGAT 1 cut(s) 1092
CseI GACGC 1 cut(s) 65
CsiI ACCWGGT 1 cut(s) 1498
Csp6I GTAC 6 cut(s) 232, 321, 648, 766, 1226, 1390
CviAII CATG 7 cut(s) 453, 486, 662, 1121, 1393, 1405, 1440
CviQI GTAC 6 cut(s) 232, 321, 648, 766, 1226, 1390
DdeI CTNAG 4 cut(s) 558, 623, 635, 1186
DpnI GATC 5 cut(s) 303, 390, 444, 1597, 1607
DpnII GATC 5 cut(s) 301, 388, 442, 1595, 1605
DriI GACNNNNNGTC 1 cut(s) 337
EaeI YGGCCR 2 cut(s) 1237, 1560
Eam1104I CTCTTC 2 cut(s) 317, 809
Eam1105I GACNNNNNGTC 1 cut(s) 337
EarI CTCTTC 2 cut(s) 317, 809
Ecl136II GAGCTC 2 cut(s) 462, 520
Eco130I CCWWGG 4 cut(s) 384, 661, 1364, 1439
Eco147I AGGCCT 1 cut(s) 1363
Eco24I GRGCYC 3 cut(s) 312, 464, 522
Eco32I GATATC 1 cut(s) 597
Eco47I GGWCC 1 cut(s) 1496
Eco53kI GAGCTC 2 cut(s) 462, 520
Eco88I CYCGRG 2 cut(s) 671, 1355
EcoICRI GAGCTC 2 cut(s) 462, 520
EcoRII CCWGG 5 cut(s) 234, 1035, 1101, 1220, 1498
EcoRV GATATC 1 cut(s) 597
EcoT14I CCWWGG 4 cut(s) 384, 661, 1364, 1439
EcoT38I GRGCYC 3 cut(s) 312, 464, 522
ErhI CCWWGG 4 cut(s) 384, 661, 1364, 1439
FaeI CATG 7 cut(s) 456, 489, 665, 1124, 1396, 1408, 1443
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FaqI GGGAC 2 cut(s) 202, 1343
FatI CATG 7 cut(s) 452, 485, 661, 1120, 1392, 1404, 1439
Fnu4HI GCNGC 5 cut(s) 285, 288, 680, 1026, 1087
FokI GGATG 4 cut(s) 187, 428, 653, 1443
FriOI GRGCYC 3 cut(s) 312, 464, 522
Fsp4HI GCNGC 5 cut(s) 285, 288, 680, 1026, 1087
FspBI CTAG 4 cut(s) 252, 567, 657, 993
GluI GCNGC 5 cut(s) 285, 288, 680, 1026, 1087
GsuI CTGGAG 2 cut(s) 1041, 1184
HaeIII GGCC 4 cut(s) 731, 1239, 1363, 1562
HapII CCGG 2 cut(s) 694, 800
HgaI GACGC 1 cut(s) 65
Hin1II CATG 7 cut(s) 456, 489, 665, 1124, 1396, 1408, 1443
HindIII AAGCTT 3 cut(s) 15, 348, 1411
HpaII CCGG 2 cut(s) 694, 800
HphI GGTGA 5 cut(s) 182, 791, 1412, 1540, 1564
Hpy166II GTNNAC 3 cut(s) 946, 1390, 1579
Hpy188I TCNGA 7 cut(s) 40, 301, 715, 1480, 1488, 1496, 1612
Hpy188III TCNNGA 7 cut(s) 547, 641, 853, 868, 1201, 1345, 1357
Hpy8I GTNNAC 3 cut(s) 946, 1390, 1579
Hpy99I CGWCG 4 cut(s) 59, 66, 131, 647
HpyAV CCTTC 5 cut(s) 19, 721, 832, 973, 1525
HpyCH4IV ACGT 4 cut(s) 222, 831, 939, 1116
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 791, 965, 1025
HpyF3I CTNAG 4 cut(s) 558, 623, 635, 1186
HpySE526I ACGT 4 cut(s) 222, 831, 939, 1116
Hsp92II CATG 7 cut(s) 456, 489, 665, 1124, 1396, 1408, 1443
KpnI GGTACC 2 cut(s) 651, 1229
Kzo9I GATC 5 cut(s) 301, 388, 442, 1595, 1605
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 1 cut(s) 1022
Lsp1109I GCAGC 2 cut(s) 666, 1037
LweI GCATC 8 cut(s) 209, 496, 675, 772, 1073, 1084, 1098, 1603
MabI ACCWGGT 1 cut(s) 1498
MaeI CTAG 4 cut(s) 252, 567, 657, 993
MaeII ACGT 4 cut(s) 222, 831, 939, 1116
MaeIII GTNAC 8 cut(s) 170, 218, 223, 325, 569, 638, 1010, 1507
MalI GATC 5 cut(s) 303, 390, 444, 1597, 1607
MbiI CCGCTC 1 cut(s) 290
MboI GATC 5 cut(s) 301, 388, 442, 1595, 1605
MboII GAAGA 8 cut(s) 304, 452, 736, 826, 847, 1329, 1381, 1580
MfeI CAATTG 1 cut(s) 531
MflI RGATCY 3 cut(s) 442, 1595, 1605
MhlI GDGCHC 4 cut(s) 312, 464, 522, 1581
MlsI TGGCCA 2 cut(s) 1239, 1562
MluCI AATT 7 cut(s) 294, 335, 531, 702, 1133, 1489, 1568
MluNI TGGCCA 2 cut(s) 1239, 1562
MlyI GAGTC 6 cut(s) 90, 233, 410, 646, 873, 1100
MmeI TCCRAC 2 cut(s) 63, 121
MnlI CCTC 8 cut(s) 112, 142, 690, 714, 944, 1208, 1353, 1364
Mox20I TGGCCA 2 cut(s) 1239, 1562
MroXI GAANNNNTTC 1 cut(s) 983
MscI TGGCCA 2 cut(s) 1239, 1562
MseI TTAA 1 cut(s) 1524
MslI CAYNNNNRTG 4 cut(s) 740, 830, 1385, 1425
Msp20I TGGCCA 2 cut(s) 1239, 1562
MspA1I CMGCKG 1 cut(s) 1019
MspI CCGG 2 cut(s) 694, 800
MspR9I CCNGG 5 cut(s) 236, 1037, 1103, 1222, 1500
MunI CAATTG 1 cut(s) 531
MvaI CCWGG 5 cut(s) 236, 1037, 1103, 1222, 1500
MvnI CGCG 1 cut(s) 653
MwoI GCNNNNNNNGC 4 cut(s) 72, 791, 965, 1025
NcoI CCATGG 2 cut(s) 661, 1439
NdeII GATC 5 cut(s) 301, 388, 442, 1595, 1605
NlaIII CATG 7 cut(s) 456, 489, 665, 1124, 1396, 1408, 1443
NlaIV GGNNCC 6 cut(s) 145, 421, 649, 1227, 1458, 1597
NmuCI GTSAC 6 cut(s) 170, 218, 223, 325, 638, 1507
NspI RCATGY 1 cut(s) 1396
OliI CACNNNNGTG 1 cut(s) 1385
PasI CCCWGGG 1 cut(s) 1221
PceI AGGCCT 1 cut(s) 1363
PciSI GCTCTTC 1 cut(s) 317
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 983
PfeI GAWTC 4 cut(s) 35, 984, 1293, 1417
PflMI CCANNNNNTGG 2 cut(s) 1042, 1235
PfoI TCCNGGA 1 cut(s) 1101
PkrI GCNGC 5 cut(s) 286, 289, 681, 1027, 1088
PleI GAGTC 6 cut(s) 89, 233, 409, 645, 872, 1100
PpsI GAGTC 6 cut(s) 89, 233, 409, 645, 872, 1100
Ppu21I YACGTR 1 cut(s) 832
PshAI GACNNNNGTC 1 cut(s) 60
Psp124BI GAGCTC 2 cut(s) 464, 522
Psp1406I AACGTT 1 cut(s) 939
Psp6I CCWGG 5 cut(s) 234, 1035, 1101, 1220, 1498
PspGI CCWGG 5 cut(s) 234, 1035, 1101, 1220, 1498
PspN4I GGNNCC 6 cut(s) 145, 421, 649, 1227, 1458, 1597
PspPI GGNCC 1 cut(s) 1496
PstI CTGCAG 1 cut(s) 469
PsuI RGATCY 3 cut(s) 442, 1595, 1605
PvuII CAGCTG 1 cut(s) 1019
RsaI GTAC 6 cut(s) 233, 322, 649, 767, 1227, 1391
RsaNI GTAC 6 cut(s) 232, 321, 648, 766, 1226, 1390
RseI CAYNNNNRTG 4 cut(s) 740, 830, 1385, 1425
SacI GAGCTC 2 cut(s) 464, 522
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 1 cut(s) 1524
SatI GCNGC 5 cut(s) 285, 288, 680, 1026, 1087
Sau3AI GATC 5 cut(s) 301, 388, 442, 1595, 1605
Sau96I GGNCC 1 cut(s) 1496
SchI GAGTC 6 cut(s) 90, 233, 410, 646, 873, 1100
ScrFI CCNGG 5 cut(s) 236, 1037, 1103, 1222, 1500
SduI GDGCHC 4 cut(s) 312, 464, 522, 1581
SexAI ACCWGGT 1 cut(s) 1498
SfaNI GCATC 8 cut(s) 209, 496, 675, 772, 1073, 1084, 1098, 1603
SfcI CTRYAG 2 cut(s) 465, 1078
SinI GGWCC 1 cut(s) 1496
SmiMI CAYNNNNRTG 4 cut(s) 740, 830, 1385, 1425
SmlI CTYRAG 3 cut(s) 521, 545, 788
SmoI CTYRAG 3 cut(s) 521, 545, 788
SpeI ACTAGT 1 cut(s) 566
Sse9I AATT 7 cut(s) 294, 335, 531, 702, 1133, 1489, 1568
SseBI AGGCCT 1 cut(s) 1363
SspMI CTAG 4 cut(s) 252, 567, 657, 993
SstI GAGCTC 2 cut(s) 464, 522
StuI AGGCCT 1 cut(s) 1363
StyD4I CCNGG 5 cut(s) 234, 1035, 1101, 1220, 1498
StyI CCWWGG 4 cut(s) 384, 661, 1364, 1439
TaiI ACGT 4 cut(s) 225, 834, 942, 1119
TaqI TCGA 8 cut(s) 54, 599, 852, 862, 867, 1055, 1092, 1272
TaqII GACCGA 1 cut(s) 1330
TasI AATT 7 cut(s) 294, 335, 531, 702, 1133, 1489, 1568
TatI WGTACW 2 cut(s) 765, 1389
TauI GCSGC 3 cut(s) 287, 290, 1089
TfiI GAWTC 4 cut(s) 35, 984, 1293, 1417
Tru1I TTAA 1 cut(s) 1524
Tru9I TTAA 1 cut(s) 1524
TscAI CASTG 5 cut(s) 37, 184, 234, 348, 961
TseFI GTSAC 6 cut(s) 170, 218, 223, 325, 638, 1507
TseI GCWGC 2 cut(s) 679, 1025
Tsp45I GTSAC 6 cut(s) 170, 218, 223, 325, 638, 1507
TspDTI ATGAA 3 cut(s) 1109, 1162, 1393
TspGWI ACGGA 4 cut(s) 124, 193, 344, 1173
TspRI CASTG 5 cut(s) 37, 184, 234, 348, 961
Van91I CCANNNNNTGG 2 cut(s) 1042, 1235
VneI GTGCAC 1 cut(s) 1577
VpaK11BI GGWCC 1 cut(s) 1496
XapI RAATTY 3 cut(s) 294, 1489, 1568
XceI RCATGY 1 cut(s) 1396
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XmnI GAANNNNTTC 1 cut(s) 983
XspI CTAG 4 cut(s) 252, 567, 657, 993
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.