FvH4_7g04920

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013785
Physical Location & Seq
Reverse (-)
5330010 .. 5333633
3624 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04920.t1

Sequence Viewer

Length: 1716 bp
ATGTCTTCCTTCAAAGCTTATGGCAAAGTCAGTGAATCCGACCAAGCCAAGCTCGACGCTCGTCGCAAGACTTGCAAGAGAGTCGCCATCATATCCATTTCCTCCATAGTTCTTGTCTGTGTCGTCGTCGCCGCCGTGGTTGGAACCGCTACTCATAACTCCAAAAAAGGTGACGACAGTGGCAAAGGGACGCTTTCCGCATCCGTCAAAGCCGTTTGTGACGTGACACTGTACCAGGACTCGTGTTATTCTAGTCTTGGTCCTGTTGTCAACACCAGCAATTTTAAGCCTGAGGATCTCTTCAAGTTGTCGATTCAAGTGGCGATTGCGGAACTATCCAAAGCCGCCGCTCAAATTTCGGATCAAGGGCTCTTCAATGGTACTGTCACGGACAAATTGTCAGTGGAAGCTTTGAAAAACTGTGGAGAGCTTCTTGGTCTTGCCTTGGATCATCTCAACGAGTCGTTGTCTGGGGATGGTTCCTTGCTTGAAGTGGTTGAAGATCTCAAAACATGGTTGAGCTCTGCAGGAACTTACCAGCAGTCATGTGTAGATGGGTTTGATGATGCAAAAGGAGAGCTCAAGGATACAATTGAAAAGTATCTCAAGACTTCTACTGAGCTAACTAGTAACAGCCTTGCCATTGTCTCTTGGATATCGAAAGTAGCTAACTCTGTGAAGCTAAGGCGGTTGCTGAGTCAAGACGGTACCGCGACTAGCCATGGCATCCCCGAGTGGCTGCACCAGACAGACCGGAGGCTAATTGAAAGTTCTGATTTGAGGAAGAAGGCCAGCATTGTTGTGGCGAAAGATGGGAGTGGTAAGTACAAGACAATAGGTGATGCACTCAAGGCTGTGCCGGACAAAAGCAAGAAGAGAACAGTCATCTACGTGAAGAAGGGGGTTTATTTCGAGAATGTTCGAGTCGAGAAATCCAAGTGGAATGTTGTGGTGGTGGGCGATGGAATGACTGCAACGATTGTGTCTGCCAGCCTCAACGTTGTGGACGGAACTCCCACCTTTTCCAGTGCAACATTTGCCGTATTTGGGAAGGGATTCGTTGCTAGAGACATTGGATTTCGTAACACAGCCGGAGCAGCCAAGCACCAGGCGGTAGCACTAATGTCGAGTGCTGACCAGACGGTGTTCTACAGATGCCGCATCGATGCGTTCCAGGACTCGCTCTACGTTCATGCCAATCGCCAATTCTACCGAGAGTGCGACATCTACGGAACAGTTGATTTCATTTTTGGAAACTCAGCAGTAGTCCTCCAGAACTGTAAGGTGTTCCCCAGGGTACCAATGACTGGCCAGCAGAACACAATCACGGCACAAGGCAAAATCGACCCTAACCAAAACACAGGAATCTCATTTCAAAACTGCACCATTTCGCCTTTCGGGGACTTGTCTTCGGTCAAGACTTACCTCGGGAGGCCTTGGAAGAACTACTCCACAACTGTGTACATGCAGACATTCATGGGAAGCTTGATTCACCCAGATGGATGGTTGCCATGGGTAGGCACAACGGCACCTGACACCATTTTCTACTCCGAGTATCAGAATTTCGGACCTGGTTCTGTGACAAAGAATAGAGTTAAGTGGAAGGGCTTGAAAACAATCACCAGCAAAGTGGCCAGCAAATTCACCGTGCACTCATTTCTTCTGGGATCCAAGTGGATCTCCGATGCGAGTGTTCCATACAAGTCCAAACTATGA

Protein Analysis

572

Amino Acids

62.11

Weight (kDa)

9.19

Isoelectric Point (pI)

24.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 68 - 215 4.8e-32 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 266 - 556 7.2e-138 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10710 AT5G04960
fragaria_vesca FvH4_7g04230 FvH4_7g04920 FvH4_7g04920
malus_domestica MD02G1266400.v1.1 MD07G1053300.v1.1
prunus_persica Prupe.2G058300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0331001
rosa_laevigata RLG00000029761
rosa_roxburghii Rroxscaffold_4G00319560
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113100 Rh1BG087100 Rh1CG110000 Rh1DG118800
rosa_wichuraiana Rw1G009220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 707, 1297
AccB1I GGYRCC 3 cut(s) 707, 1297, 1528
AccB7I CCANNNNNTGG 1 cut(s) 1307
AccBSI CCGCTC 1 cut(s) 350
AccII CGCG 1 cut(s) 713
AclI AACGTT 1 cut(s) 999
AclWI GGATC 6 cut(s) 303, 369, 456, 1662, 1675, 1685
AcoI YGGCCR 2 cut(s) 1309, 1632
AcsI RAATTY 3 cut(s) 354, 1561, 1640
AfaI GTAC 6 cut(s) 233, 382, 709, 827, 1299, 1463
AfiI CCNNNNNNNGG 3 cut(s) 1047, 1307, 1517
AhdI GACNNNNNGTC 1 cut(s) 397
AhlI ACTAGT 1 cut(s) 626
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 5 cut(s) 234, 1107, 1173, 1292, 1570
AjuI GAANNNNNNNTTGG 2 cut(s) 1190, 1222
AleI CACNNNNGTG 1 cut(s) 1457
Alw21I GWGCWC 3 cut(s) 524, 582, 1653
Alw26I GTCTC 2 cut(s) 652, 1062
Alw44I GTGCAC 1 cut(s) 1649
AlwI GGATC 6 cut(s) 303, 369, 456, 1662, 1675, 1685
Ama87I CYCGRG 2 cut(s) 731, 1427
AoxI GGCC 4 cut(s) 789, 1309, 1433, 1632
ApaLI GTGCAC 1 cut(s) 1649
ApeKI GCWGC 2 cut(s) 739, 1097
ApoI RAATTY 3 cut(s) 354, 1561, 1640
Asp700I GAANNNNTTC 1 cut(s) 1055
Asp718I GGTACC 2 cut(s) 707, 1297
AspS9I GGNCC 2 cut(s) 260, 1568
AsuHPI GGTGA 5 cut(s) 182, 851, 1484, 1612, 1636
AvaI CYCGRG 2 cut(s) 731, 1427
AvaII GGWCC 2 cut(s) 260, 1568
AxyI CCTNAGG 1 cut(s) 291
BaeGI GKGCMC 1 cut(s) 1653
BalI TGGCCA 2 cut(s) 1311, 1634
BamHI GGATCC 1 cut(s) 1667
BanI GGYRCC 3 cut(s) 707, 1297, 1528
BanII GRGCYC 3 cut(s) 372, 524, 582
BauI CACGAG 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 1401
Bbv12I GWGCWC 3 cut(s) 524, 582, 1653
BbvI GCAGC 2 cut(s) 726, 1109
BccI CCATC 7 cut(s) 95, 470, 548, 806, 956, 1493, 1497
BceAI ACGGC 5 cut(s) 119, 197, 1025, 1344, 1542
BcgI CGANNNNNNTGC 4 cut(s) 64, 98, 1107, 1141
BciT130I CCWGG 5 cut(s) 236, 1109, 1175, 1294, 1572
BciVI GTATCC 1 cut(s) 580
BcoDI GTCTC 2 cut(s) 652, 1062
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 4 cut(s) 252, 627, 717, 1065
BfmI CTRYAG 2 cut(s) 525, 1150
BfuI GTATCC 1 cut(s) 580
BglII AGATCT 1 cut(s) 502
BisI GCNGC 6 cut(s) 132, 345, 348, 740, 1098, 1159
BlsI GCNGC 6 cut(s) 133, 346, 349, 741, 1099, 1160
Bme1390I CCNGG 5 cut(s) 236, 1109, 1175, 1294, 1572
Bme18I GGWCC 2 cut(s) 260, 1568
BmeRI GACNNNNNGTC 1 cut(s) 397
BmeT110I CYCGRG 2 cut(s) 731, 1427
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 260, 1568
BmiI GGNNCC 6 cut(s) 145, 481, 709, 1299, 1530, 1669
BmrFI CCNGG 5 cut(s) 236, 1109, 1175, 1294, 1572
BmsI GCATC 8 cut(s) 209, 556, 735, 832, 1145, 1156, 1170, 1675
BoxI GACNNNNGTC 1 cut(s) 60
BpiI GAAGAC 1 cut(s) 1401
BpmI CTGGAG 1 cut(s) 1256
Bpu10I CCTNAGC 1 cut(s) 683
BpuEI CTTGAG 3 cut(s) 566, 590, 833
Bsa29I ATCGAT 1 cut(s) 1164
BsaAI YACGTR 1 cut(s) 892
BsaJI CCNNGG 8 cut(s) 135, 444, 721, 1292, 1293, 1426, 1436, 1511
BsaWI WCCGGW 1 cut(s) 753
Bsc4I CCNNNNNNNGG 3 cut(s) 1047, 1307, 1517
Bse1I ACTGG 2 cut(s) 1026, 1312
Bse21I CCTNAGG 1 cut(s) 291
BseBI CCWGG 5 cut(s) 236, 1109, 1175, 1294, 1572
BseCI ATCGAT 1 cut(s) 1164
BseDI CCNNGG 8 cut(s) 135, 444, 721, 1292, 1293, 1426, 1436, 1511
BseGI GGATG 4 cut(s) 200, 481, 726, 1508
BseLI CCNNNNNNNGG 3 cut(s) 1047, 1307, 1517
BseMII CTCAG 4 cut(s) 282, 609, 686, 1272
BseNI ACTGG 2 cut(s) 1026, 1312
BseSI GKGCMC 1 cut(s) 1653
BseXI GCAGC 2 cut(s) 726, 1109
BsgI GTGCAG 2 cut(s) 725, 1366
Bsh1236I CGCG 1 cut(s) 713
BshFI GGCC 4 cut(s) 791, 1311, 1435, 1634
BshNI GGYRCC 3 cut(s) 707, 1297, 1528
BshVI ATCGAT 1 cut(s) 1164
BsiHKAI GWGCWC 3 cut(s) 524, 582, 1653
BsiHKCI CYCGRG 2 cut(s) 731, 1427
BsiSI CCGG 3 cut(s) 754, 860, 1092
BslFI GGGAC 2 cut(s) 202, 1415
BslI CCNNNNNNNGG 3 cut(s) 1047, 1307, 1517
BsmAI GTCTC 2 cut(s) 652, 1062
BsmFI GGGAC 2 cut(s) 202, 1415
BsnI GGCC 4 cut(s) 791, 1311, 1435, 1634
BsoBI CYCGRG 2 cut(s) 731, 1427
Bsp1286I GDGCHC 4 cut(s) 372, 524, 582, 1653
Bsp1407I TGTACA 1 cut(s) 1461
Bsp143I GATC 6 cut(s) 295, 361, 448, 502, 1667, 1677
Bsp19I CCATGG 2 cut(s) 721, 1511
BspANI GGCC 4 cut(s) 791, 1311, 1435, 1634
BspCNI CTCAG 4 cut(s) 283, 610, 687, 1271
BspDI ATCGAT 1 cut(s) 1164
BspFNI CGCG 1 cut(s) 713
BspLI GGNNCC 6 cut(s) 145, 481, 709, 1299, 1530, 1669
BspMAI CTGCAG 1 cut(s) 529
BspPI GGATC 6 cut(s) 303, 369, 456, 1662, 1675, 1685
BspQI GCTCTTC 1 cut(s) 377
BspT107I GGYRCC 3 cut(s) 707, 1297, 1528
BsrBI CCGCTC 1 cut(s) 350
BsrGI TGTACA 1 cut(s) 1461
BsrI ACTGG 2 cut(s) 1026, 1312
BssECI CCNNGG 8 cut(s) 135, 444, 721, 1292, 1293, 1426, 1436, 1511
BssMI GATC 6 cut(s) 295, 361, 448, 502, 1667, 1677
BssSI CACGAG 1 cut(s) 241
BssT1I CCWWGG 4 cut(s) 444, 721, 1436, 1511
Bst2BI CACGAG 1 cut(s) 241
Bst2UI CCWGG 5 cut(s) 236, 1109, 1175, 1294, 1572
Bst6I CTCTTC 3 cut(s) 305, 377, 869
BstAPI GCANNNNNTGC 2 cut(s) 72, 1037
BstAUI TGTACA 1 cut(s) 1461
BstBAI YACGTR 1 cut(s) 892
BstC8I GCNNGC 4 cut(s) 793, 991, 1313, 1636
BstDEI CTNAG 5 cut(s) 291, 618, 683, 695, 1258
BstDSI CCRYGG 3 cut(s) 135, 721, 1511
BstF5I GGATG 4 cut(s) 200, 481, 726, 1508
BstFNI CGCG 1 cut(s) 713
BstKTI GATC 6 cut(s) 298, 364, 451, 505, 1670, 1680
BstMAI GTCTC 2 cut(s) 652, 1062
BstMBI GATC 6 cut(s) 295, 361, 448, 502, 1667, 1677
BstMWI GCNNNNNNNGC 4 cut(s) 72, 851, 1037, 1097
BstNI CCWGG 5 cut(s) 236, 1109, 1175, 1294, 1572
BstNSI RCATGY 1 cut(s) 1468
BstPAI GACNNNNGTC 1 cut(s) 60
BstSCI CCNGG 5 cut(s) 234, 1107, 1173, 1292, 1570
BstSFI CTRYAG 2 cut(s) 525, 1150
BstSLI GKGCMC 1 cut(s) 1653
BstUI CGCG 1 cut(s) 713
BstV1I GCAGC 2 cut(s) 726, 1109
BstV2I GAAGAC 1 cut(s) 1401
BstX2I RGATCY 4 cut(s) 295, 502, 1667, 1677
BstXI CCANNNNNNTGG 2 cut(s) 1503, 1630
BstYI RGATCY 4 cut(s) 295, 502, 1667, 1677
Bsu15I ATCGAT 1 cut(s) 1164
Bsu36I CCTNAGG 1 cut(s) 291
BsuI GTATCC 1 cut(s) 580
BsuRI GGCC 4 cut(s) 791, 1311, 1435, 1634
BsuTUI ATCGAT 1 cut(s) 1164
BtgI CCRYGG 3 cut(s) 135, 721, 1511
BtgZI GCGATG 1 cut(s) 975
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 4 cut(s) 200, 481, 726, 1508
BtsIMutI CAGTG 5 cut(s) 37, 184, 227, 408, 1033
Cac8I GCNNGC 4 cut(s) 793, 991, 1313, 1636
Cfr13I GGNCC 2 cut(s) 260, 1568
ClaI ATCGAT 1 cut(s) 1164
CseI GACGC 2 cut(s) 65, 199
CsiI ACCWGGT 1 cut(s) 1570
Csp6I GTAC 6 cut(s) 232, 381, 708, 826, 1298, 1462
CviAII CATG 7 cut(s) 513, 546, 722, 1193, 1465, 1477, 1512
CviQI GTAC 6 cut(s) 232, 381, 708, 826, 1298, 1462
DdeI CTNAG 5 cut(s) 291, 618, 683, 695, 1258
DpnI GATC 6 cut(s) 297, 363, 450, 504, 1669, 1679
DpnII GATC 6 cut(s) 295, 361, 448, 502, 1667, 1677
DriI GACNNNNNGTC 1 cut(s) 397
EaeI YGGCCR 2 cut(s) 1309, 1632
Eam1104I CTCTTC 3 cut(s) 305, 377, 869
Eam1105I GACNNNNNGTC 1 cut(s) 397
EarI CTCTTC 3 cut(s) 305, 377, 869
Ecl136II GAGCTC 2 cut(s) 522, 580
Eco130I CCWWGG 4 cut(s) 444, 721, 1436, 1511
Eco147I AGGCCT 1 cut(s) 1435
Eco24I GRGCYC 3 cut(s) 372, 524, 582
Eco32I GATATC 1 cut(s) 657
Eco47I GGWCC 2 cut(s) 260, 1568
Eco53kI GAGCTC 2 cut(s) 522, 580
Eco81I CCTNAGG 1 cut(s) 291
Eco88I CYCGRG 2 cut(s) 731, 1427
EcoICRI GAGCTC 2 cut(s) 522, 580
EcoRII CCWGG 5 cut(s) 234, 1107, 1173, 1292, 1570
EcoRV GATATC 1 cut(s) 657
EcoT14I CCWWGG 4 cut(s) 444, 721, 1436, 1511
EcoT38I GRGCYC 3 cut(s) 372, 524, 582
ErhI CCWWGG 4 cut(s) 444, 721, 1436, 1511
FaeI CATG 7 cut(s) 516, 549, 725, 1196, 1468, 1480, 1515
FalI AAGNNNNNCTT 2 cut(s) 177, 209
FaqI GGGAC 2 cut(s) 202, 1415
FatI CATG 7 cut(s) 512, 545, 721, 1192, 1464, 1476, 1511
Fnu4HI GCNGC 6 cut(s) 132, 345, 348, 740, 1098, 1159
FokI GGATG 4 cut(s) 187, 488, 713, 1515
FriOI GRGCYC 3 cut(s) 372, 524, 582
Fsp4HI GCNGC 6 cut(s) 132, 345, 348, 740, 1098, 1159
FspBI CTAG 4 cut(s) 252, 627, 717, 1065
GluI GCNGC 6 cut(s) 132, 345, 348, 740, 1098, 1159
GsuI CTGGAG 1 cut(s) 1256
HaeIII GGCC 4 cut(s) 791, 1311, 1435, 1634
HapII CCGG 3 cut(s) 754, 860, 1092
HgaI GACGC 2 cut(s) 65, 199
Hin1II CATG 7 cut(s) 516, 549, 725, 1196, 1468, 1480, 1515
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 3 cut(s) 15, 408, 1483
HpaII CCGG 3 cut(s) 754, 860, 1092
HphI GGTGA 5 cut(s) 182, 851, 1484, 1612, 1636
Hpy166II GTNNAC 4 cut(s) 271, 1006, 1462, 1651
Hpy188I TCNGA 7 cut(s) 40, 361, 775, 1552, 1560, 1568, 1684
Hpy188III TCNNGA 7 cut(s) 607, 701, 913, 928, 1273, 1417, 1429
Hpy8I GTNNAC 4 cut(s) 271, 1006, 1462, 1651
Hpy99I CGWCG 4 cut(s) 59, 66, 128, 131
HpyAV CCTTC 5 cut(s) 19, 781, 892, 1045, 1597
HpyCH4IV ACGT 4 cut(s) 222, 891, 999, 1188
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 851, 1037, 1097
HpyF3I CTNAG 5 cut(s) 291, 618, 683, 695, 1258
HpySE526I ACGT 4 cut(s) 222, 891, 999, 1188
Hsp92II CATG 7 cut(s) 516, 549, 725, 1196, 1468, 1480, 1515
KpnI GGTACC 2 cut(s) 711, 1301
Kzo9I GATC 6 cut(s) 295, 361, 448, 502, 1667, 1677
LguI GCTCTTC 1 cut(s) 377
LmnI GCTCC 1 cut(s) 1094
Lsp1109I GCAGC 2 cut(s) 726, 1109
LweI GCATC 8 cut(s) 209, 556, 735, 832, 1145, 1156, 1170, 1675
MabI ACCWGGT 1 cut(s) 1570
MaeI CTAG 4 cut(s) 252, 627, 717, 1065
MaeII ACGT 4 cut(s) 222, 891, 999, 1188
MaeIII GTNAC 7 cut(s) 170, 218, 223, 385, 629, 1082, 1579
MalI GATC 6 cut(s) 297, 363, 450, 504, 1669, 1679
MbiI CCGCTC 1 cut(s) 350
MboI GATC 6 cut(s) 295, 361, 448, 502, 1667, 1677
MboII GAAGA 9 cut(s) 292, 364, 512, 796, 886, 907, 1401, 1453, 1652
MfeI CAATTG 1 cut(s) 591
MflI RGATCY 4 cut(s) 295, 502, 1667, 1677
MhlI GDGCHC 4 cut(s) 372, 524, 582, 1653
MlsI TGGCCA 2 cut(s) 1311, 1634
MluCI AATT 8 cut(s) 280, 354, 395, 591, 762, 1205, 1561, 1640
MluNI TGGCCA 2 cut(s) 1311, 1634
MlyI GAGTC 6 cut(s) 90, 233, 470, 706, 933, 1172
MmeI TCCRAC 2 cut(s) 63, 121
MnlI CCTC 8 cut(s) 112, 286, 750, 774, 1004, 1280, 1425, 1436
Mox20I TGGCCA 2 cut(s) 1311, 1634
MroXI GAANNNNTTC 1 cut(s) 1055
MscI TGGCCA 2 cut(s) 1311, 1634
MseI TTAA 2 cut(s) 285, 1596
MslI CAYNNNNRTG 4 cut(s) 800, 890, 1457, 1497
Msp20I TGGCCA 2 cut(s) 1311, 1634
MspI CCGG 3 cut(s) 754, 860, 1092
MspR9I CCNGG 5 cut(s) 236, 1109, 1175, 1294, 1572
MunI CAATTG 1 cut(s) 591
MvaI CCWGG 5 cut(s) 236, 1109, 1175, 1294, 1572
MvnI CGCG 1 cut(s) 713
MwoI GCNNNNNNNGC 4 cut(s) 72, 851, 1037, 1097
NcoI CCATGG 2 cut(s) 721, 1511
NdeII GATC 6 cut(s) 295, 361, 448, 502, 1667, 1677
NlaIII CATG 7 cut(s) 516, 549, 725, 1196, 1468, 1480, 1515
NlaIV GGNNCC 6 cut(s) 145, 481, 709, 1299, 1530, 1669
NmuCI GTSAC 5 cut(s) 170, 218, 223, 385, 1579
NspI RCATGY 1 cut(s) 1468
OliI CACNNNNGTG 1 cut(s) 1457
PasI CCCWGGG 1 cut(s) 1293
PceI AGGCCT 1 cut(s) 1435
PciSI GCTCTTC 1 cut(s) 377
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 1055
PfeI GAWTC 5 cut(s) 35, 313, 1056, 1365, 1489
PflMI CCANNNNNTGG 1 cut(s) 1307
PfoI TCCNGGA 1 cut(s) 1173
PkrI GCNGC 6 cut(s) 133, 346, 349, 741, 1099, 1160
PleI GAGTC 6 cut(s) 89, 233, 469, 705, 932, 1172
PpsI GAGTC 6 cut(s) 89, 233, 469, 705, 932, 1172
Ppu21I YACGTR 1 cut(s) 892
PshAI GACNNNNGTC 1 cut(s) 60
Psp124BI GAGCTC 2 cut(s) 524, 582
Psp1406I AACGTT 1 cut(s) 999
Psp6I CCWGG 5 cut(s) 234, 1107, 1173, 1292, 1570
PspGI CCWGG 5 cut(s) 234, 1107, 1173, 1292, 1570
PspN4I GGNNCC 6 cut(s) 145, 481, 709, 1299, 1530, 1669
PspPI GGNCC 2 cut(s) 260, 1568
PstI CTGCAG 1 cut(s) 529
PsuI RGATCY 4 cut(s) 295, 502, 1667, 1677
RsaI GTAC 6 cut(s) 233, 382, 709, 827, 1299, 1463
RsaNI GTAC 6 cut(s) 232, 381, 708, 826, 1298, 1462
RseI CAYNNNNRTG 4 cut(s) 800, 890, 1457, 1497
SacI GAGCTC 2 cut(s) 524, 582
SapI GCTCTTC 1 cut(s) 377
SaqAI TTAA 2 cut(s) 285, 1596
SatI GCNGC 6 cut(s) 132, 345, 348, 740, 1098, 1159
Sau3AI GATC 6 cut(s) 295, 361, 448, 502, 1667, 1677
Sau96I GGNCC 2 cut(s) 260, 1568
SchI GAGTC 6 cut(s) 90, 233, 470, 706, 933, 1172
ScrFI CCNGG 5 cut(s) 236, 1109, 1175, 1294, 1572
SduI GDGCHC 4 cut(s) 372, 524, 582, 1653
SexAI ACCWGGT 1 cut(s) 1570
SfaNI GCATC 8 cut(s) 209, 556, 735, 832, 1145, 1156, 1170, 1675
SfcI CTRYAG 2 cut(s) 525, 1150
SinI GGWCC 2 cut(s) 260, 1568
SmiMI CAYNNNNRTG 4 cut(s) 800, 890, 1457, 1497
SmlI CTYRAG 3 cut(s) 581, 605, 848
SmoI CTYRAG 3 cut(s) 581, 605, 848
SpeI ACTAGT 1 cut(s) 626
Sse9I AATT 8 cut(s) 280, 354, 395, 591, 762, 1205, 1561, 1640
SseBI AGGCCT 1 cut(s) 1435
SspMI CTAG 4 cut(s) 252, 627, 717, 1065
SstI GAGCTC 2 cut(s) 524, 582
StuI AGGCCT 1 cut(s) 1435
StyD4I CCNGG 5 cut(s) 234, 1107, 1173, 1292, 1570
StyI CCWWGG 4 cut(s) 444, 721, 1436, 1511
TaiI ACGT 4 cut(s) 225, 894, 1002, 1191
TaqI TCGA 9 cut(s) 54, 311, 659, 912, 922, 927, 1127, 1164, 1344
TaqII GACCGA 1 cut(s) 1402
TasI AATT 8 cut(s) 280, 354, 395, 591, 762, 1205, 1561, 1640
TatI WGTACW 2 cut(s) 825, 1461
TauI GCSGC 4 cut(s) 134, 347, 350, 1161
TfiI GAWTC 5 cut(s) 35, 313, 1056, 1365, 1489
Tru1I TTAA 2 cut(s) 285, 1596
Tru9I TTAA 2 cut(s) 285, 1596
TscAI CASTG 5 cut(s) 37, 184, 234, 408, 1033
TseFI GTSAC 5 cut(s) 170, 218, 223, 385, 1579
TseI GCWGC 2 cut(s) 739, 1097
Tsp45I GTSAC 5 cut(s) 170, 218, 223, 385, 1579
TspDTI ATGAA 3 cut(s) 1181, 1234, 1465
TspGWI ACGGA 4 cut(s) 193, 404, 1023, 1245
TspRI CASTG 5 cut(s) 37, 184, 234, 408, 1033
Van91I CCANNNNNTGG 1 cut(s) 1307
VneI GTGCAC 1 cut(s) 1649
VpaK11BI GGWCC 2 cut(s) 260, 1568
XapI RAATTY 3 cut(s) 354, 1561, 1640
XceI RCATGY 1 cut(s) 1468
XcmI CCANNNNNNNNNTGG 1 cut(s) 799
XmnI GAANNNNTTC 1 cut(s) 1055
XspI CTAG 4 cut(s) 252, 627, 717, 1065
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.