MD07G1053300.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013785
Physical Location & Seq
Reverse (-)
4926287 .. 4928369
2083 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1053300.v1.1.491

Sequence Viewer

Length: 1719 bp
ATGTCTTCCTTCAAATCCTATGGAAAGGTTGATGAATCCGAGCAAGCCCGGCTCGACGCTTGTCGGAAAACTCGAAAAAGAGTAGCCATCATAAGCCTATCATCCATGGTTCTTGTCTGTATCGTTGTTGCTGCTGTGGTTGGAACCACCGTTCATAATAAATCTGGTGGGAATTCTAAGGGCTCGGGCATCTCCACTTCTGTCAAGGCTGTTTGCGATGTGACCTTGTACCAGGACTCGTGTTACAGCAGTCTTAGTCCTCTGGTTAACTCCAACAATGTTAAGCCTGAGGAGCTTTTCAAGTTGGCTGTTCAGGTGGCTATCAATGAATTGACGAAAGCCGCTAGTCATTTTTCGGCTGGTGGGGTTCTTAATGCGACTGCCAAGGACAGAAAGTCCGTGGCAGCTTTGAAAGATTGTCAAGACCTTTTGAGTCTGGCATTGGATCATCTCAACGACACATTGTCCGATGCTAACTCGTTGGTTGATGTGTTTGATGATCTCACGACTTGGCTGAGCTCTTCAGGCACATATCAGCAGACTTGCATTGAAGGTTTTGATGAAGTAAAAGGAGAACTCAAGGATAAAGTTATCGGCTACTTGAAGAACTCCACTGAGTTAACTAGCAACAGTCTTGCCATAGTCTCTTGGATTTCCAAAATCCTTAACTCGGTGAAGCTAAGGCGACTGCTGACACACCCTGACCCCACAGATAGCTACAGCCAGGGTGTGCCAGAGTGGCTGCATCGGAAGGACCGCAAGCTGCTTCAGAGTTCGGATTTGAGGAAGAAGGCAGACATTGTCGTGGCGCGTGATGGCAGTGGCAAGTACAAAACAATTCACGATGCACTGAAGGCTGTTCCGAATAAAAGCAAGAAGAGAACAATCATCTACGTGAAGAAGGGGGTTTATTACGGGAATGTGAGAGTTGAGAAATCCAAGTGGAATGTTTTGGTGGTCGGTGATGGTTCGACTGCAACTATTGTATCTGCCAAACTCAACGTTGTGGATGGAACTCCCACATTTTCCACTGCCACATTCGCCGTTTTTGGAAGGGGATTCATTGCTCGGGACATTGGATTTCGCAACACTGCCGGACCAGCCAAGCACCAGGCAGTAGCCTTGATGTCAACCGCCGATCAGTCAGTCTTCTATAGGTGCCACATTGATGCGTTCCAGGACACTCTCTATGCTCACTCCAATCGCCAGTTCTACCGCGAATGCAACATTTATGGAACGGTTGACTTCATTTTCGGGAACTCAGCAGTTGTGCTCCAGAACTGCAACATAATGCCCAAAGTTCCCATGCCAGGCCAGCAGAACACCATCACAGCTCAAGGGAAAATTGATCCCAATCAAAATACAGGAATTTCAATTCACAATTGCAGCATATTGCCCTTCGGAGACTTGTCCAGGGTCAAAACTTACCTCGGGAGGCCATGGAAGAATCACTCCACAACTGTGTACATGCAAACATACATGGGAAGCCTCATCAACCCTAATGGATGGATGCCATGGGTGGGCACAAGCGCTCCGGATACCATATTTTACTCGGAATTTCAAAATTTTGGACCTGGTTCTTCCACAGAGAATAGAGTTAAATGGAAGGGTTTGAGATCCATCAACCAAAAAGCAGCCAGCAAATTTACAGTGCATTCATTTCTCCAAGGAACTAAGTGGATCTCTGATGCTGGTGTTACCTACAAAGCGAGCCTCTGA

Protein Analysis

573

Amino Acids

62.72

Weight (kDa)

9.4

Isoelectric Point (pI)

33.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 67 - 214 1.8e-33 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 266 - 557 4.7e-139 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10710 AT5G04960
fragaria_vesca FvH4_7g04230 FvH4_7g04920 FvH4_7g04920
malus_domestica MD02G1266400.v1.1 MD07G1053300.v1.1
prunus_persica Prupe.2G058300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0331001
rosa_laevigata RLG00000029761
rosa_roxburghii Rroxscaffold_4G00319560
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113100 Rh1BG087100 Rh1CG110000 Rh1DG118800
rosa_wichuraiana Rw1G009220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1158
AccII CGCG 2 cut(s) 811, 1218
AccIII TCCGGA 1 cut(s) 1534
AciI CCGC 4 cut(s) 342, 757, 1134, 1216
AclI AACGTT 1 cut(s) 1002
AclWI GGATC 4 cut(s) 453, 1343, 1611, 1688
AcsI RAATTY 5 cut(s) 172, 1368, 1556, 1564, 1643
AcuI CTGAAG 3 cut(s) 507, 752, 872
AfaI GTAC 3 cut(s) 230, 830, 1466
AfeI AGCGCT 1 cut(s) 1531
AfiI CCNNNNNNNGG 3 cut(s) 670, 1310, 1520
AgsI TTSAA 7 cut(s) 13, 301, 412, 551, 604, 1374, 1562
AhdI GACNNNNNGTC 2 cut(s) 394, 463
AjnI CCWGG 7 cut(s) 231, 723, 1110, 1176, 1309, 1412, 1573
AjuI GAANNNNNNNTTGG 2 cut(s) 1193, 1225
AleI CACNNNNGTG 1 cut(s) 1460
AloI GAACNNNNNNTCC 2 cut(s) 135, 167
AluBI AGCT 7 cut(s) 295, 407, 519, 679, 717, 763, 1334
AluI AGCT 7 cut(s) 295, 407, 519, 679, 717, 763, 1334
Alw21I GWGCWC 2 cut(s) 521, 1275
Alw26I GTCTC 2 cut(s) 649, 1398
AlwI GGATC 4 cut(s) 453, 1343, 1611, 1688
Ama87I CYCGRG 3 cut(s) 184, 1068, 1430
Aor13HI TCCGGA 1 cut(s) 1534
Aor51HI AGCGCT 1 cut(s) 1531
AoxI GGCC 2 cut(s) 1312, 1436
ApeKI GCWGC 6 cut(s) 131, 404, 742, 763, 1386, 1634
ApoI RAATTY 5 cut(s) 172, 1368, 1556, 1564, 1643
ArsI GACNNNNNNTTYG 2 cut(s) 1235, 1267
AspLEI GCGC 2 cut(s) 811, 1532
AspS9I GGNCC 3 cut(s) 754, 1097, 1571
AsuC2I CCSGG 1 cut(s) 49
AsuHPI GGTGA 2 cut(s) 685, 974
AvaI CYCGRG 3 cut(s) 184, 1068, 1430
AvaII GGWCC 3 cut(s) 754, 1097, 1571
AxyI CCTNAGG 1 cut(s) 288
BaeGI GKGCMC 1 cut(s) 1526
BaeI ACNNNNGTAYC 2 cut(s) 969, 1002
BanI GGYRCC 1 cut(s) 1158
BanII GRGCYC 2 cut(s) 185, 521
BauI CACGAG 1 cut(s) 238
BbsI GAAGAC 1 cut(s) 1141
Bbv12I GWGCWC 2 cut(s) 521, 1275
BbvI GCAGC 6 cut(s) 118, 416, 729, 750, 1398, 1646
BccI CCATC 7 cut(s) 95, 809, 959, 1004, 1334, 1500, 1628
BceAI ACGGC 1 cut(s) 1028
BciT130I CCWGG 7 cut(s) 233, 725, 1112, 1178, 1311, 1414, 1575
BciVI GTATCC 1 cut(s) 1531
BcnI CCSGG 1 cut(s) 49
BcoDI GTCTC 2 cut(s) 649, 1398
BfaI CTAG 2 cut(s) 345, 624
BfmI CTRYAG 2 cut(s) 718, 1153
BfoI RGCGCY 1 cut(s) 1533
BfuI GTATCC 1 cut(s) 1531
BglI GCCNNNNNGGC 1 cut(s) 739
BisI GCNGC 7 cut(s) 132, 342, 405, 743, 764, 1387, 1635
BlpI GCTNAGC 1 cut(s) 515
BlsI GCNGC 7 cut(s) 133, 343, 406, 744, 765, 1388, 1636
Bme1390I CCNGG 8 cut(s) 49, 233, 725, 1112, 1178, 1311, 1414, 1575
Bme18I GGWCC 3 cut(s) 754, 1097, 1571
BmeRI GACNNNNNGTC 2 cut(s) 394, 463
BmeT110I CYCGRG 3 cut(s) 184, 1068, 1430
BmgT120I GGNCC 3 cut(s) 754, 1097, 1571
BmiI GGNNCC 2 cut(s) 145, 1160
BmrFI CCNGG 8 cut(s) 49, 233, 725, 1112, 1178, 1311, 1414, 1575
BmsI GCATC 7 cut(s) 198, 460, 754, 835, 1159, 1500, 1678
BoxI GACNNNNGTC 1 cut(s) 60
BpiI GAAGAC 1 cut(s) 1141
BpmI CTGGAG 1 cut(s) 1259
Bpu10I CCTNAGC 1 cut(s) 680
Bpu1102I GCTNAGC 1 cut(s) 515
BpuEI CTTGAG 2 cut(s) 563, 1320
BpuMI CCSGG 1 cut(s) 49
BsaAI YACGTR 1 cut(s) 895
BsaBI GATNNNNATC 1 cut(s) 1353
BsaJI CCNNGG 9 cut(s) 105, 384, 399, 724, 1413, 1429, 1439, 1514, 1666
BsaWI WCCGGW 1 cut(s) 1534
BsaXI ACNNNNNCTCC 2 cut(s) 1516, 1546
Bsc4I CCNNNNNNNGG 3 cut(s) 670, 1310, 1520
Bse1I ACTGG 1 cut(s) 1207
Bse21I CCTNAGG 1 cut(s) 288
Bse3DI GCAATG 1 cut(s) 1062
Bse8I GATNNNNATC 1 cut(s) 1353
BseAI TCCGGA 1 cut(s) 1534
BseBI CCWGG 7 cut(s) 233, 725, 1112, 1178, 1311, 1414, 1575
BseDI CCNNGG 9 cut(s) 105, 384, 399, 724, 1413, 1429, 1439, 1514, 1666
BseGI GGATG 4 cut(s) 101, 1015, 1511, 1515
BseJI GATNNNNATC 1 cut(s) 1353
BseLI CCNNNNNNNGG 3 cut(s) 670, 1310, 1520
BseMI GCAATG 1 cut(s) 1062
BseMII CTCAG 4 cut(s) 279, 506, 606, 1275
BseNI ACTGG 1 cut(s) 1207
BseRI GAGGAG 1 cut(s) 305
BseSI GKGCMC 1 cut(s) 1526
BseXI GCAGC 6 cut(s) 118, 416, 729, 750, 1398, 1646
Bsh1236I CGCG 2 cut(s) 811, 1218
BshFI GGCC 2 cut(s) 1314, 1438
BshNI GGYRCC 1 cut(s) 1158
BsiHKAI GWGCWC 2 cut(s) 521, 1275
BsiHKCI CYCGRG 3 cut(s) 184, 1068, 1430
BsiSI CCGG 3 cut(s) 49, 1095, 1535
BslFI GGGAC 1 cut(s) 1085
BslI CCNNNNNNNGG 3 cut(s) 670, 1310, 1520
BsmAI GTCTC 2 cut(s) 649, 1398
BsmFI GGGAC 1 cut(s) 1085
BsmI GAATGC 2 cut(s) 1226, 1654
BsnI GGCC 2 cut(s) 1314, 1438
BsoBI CYCGRG 3 cut(s) 184, 1068, 1430
Bsp1286I GDGCHC 4 cut(s) 185, 521, 1275, 1526
Bsp13I TCCGGA 1 cut(s) 1534
Bsp1407I TGTACA 1 cut(s) 1464
Bsp143I GATC 6 cut(s) 445, 499, 1138, 1348, 1616, 1680
Bsp1720I GCTNAGC 1 cut(s) 515
Bsp19I CCATGG 3 cut(s) 105, 1439, 1514
BspACI CCGC 4 cut(s) 342, 757, 1134, 1216
BspANI GGCC 2 cut(s) 1314, 1438
BspCNI CTCAG 4 cut(s) 280, 507, 607, 1274
BspEI TCCGGA 1 cut(s) 1534
BspFNI CGCG 2 cut(s) 811, 1218
BspLI GGNNCC 2 cut(s) 145, 1160
BspPI GGATC 4 cut(s) 453, 1343, 1611, 1688
BspQI GCTCTTC 1 cut(s) 526
BspT107I GGYRCC 1 cut(s) 1158
BsrDI GCAATG 1 cut(s) 1062
BsrGI TGTACA 1 cut(s) 1464
BsrI ACTGG 1 cut(s) 1207
BssECI CCNNGG 9 cut(s) 105, 384, 399, 724, 1413, 1429, 1439, 1514, 1666
BssMI GATC 6 cut(s) 445, 499, 1138, 1348, 1616, 1680
BssSI CACGAG 1 cut(s) 238
BssT1I CCWWGG 5 cut(s) 105, 384, 1439, 1514, 1666
Bst2BI CACGAG 1 cut(s) 238
Bst2UI CCWGG 7 cut(s) 233, 725, 1112, 1178, 1311, 1414, 1575
Bst4CI ACNGT 5 cut(s) 151, 632, 1240, 1462, 1651
Bst6I CTCTTC 2 cut(s) 526, 872
BstAUI TGTACA 1 cut(s) 1464
BstBAI YACGTR 1 cut(s) 895
BstC8I GCNNGC 5 cut(s) 45, 761, 1316, 1639, 1711
BstDEI CTNAG 8 cut(s) 177, 254, 288, 515, 615, 680, 1261, 1674
BstDSI CCRYGG 4 cut(s) 105, 399, 1439, 1514
BstF5I GGATG 4 cut(s) 101, 1015, 1511, 1515
BstFNI CGCG 2 cut(s) 811, 1218
BstH2I RGCGCY 1 cut(s) 1533
BstHHI GCGC 2 cut(s) 811, 1532
BstKTI GATC 6 cut(s) 448, 502, 1141, 1351, 1619, 1683
BstMAI GTCTC 2 cut(s) 649, 1398
BstMBI GATC 6 cut(s) 445, 499, 1138, 1348, 1616, 1680
BstMWI GCNNNNNNNGC 8 cut(s) 49, 292, 525, 739, 854, 1040, 1100, 1315
BstNI CCWGG 7 cut(s) 233, 725, 1112, 1178, 1311, 1414, 1575
BstNSI RCATGY 1 cut(s) 1471
BstPAI GACNNNNGTC 1 cut(s) 60
BstSCI CCNGG 8 cut(s) 47, 231, 723, 1110, 1176, 1309, 1412, 1573
BstSFI CTRYAG 2 cut(s) 718, 1153
BstSLI GKGCMC 1 cut(s) 1526
BstUI CGCG 2 cut(s) 811, 1218
BstV1I GCAGC 6 cut(s) 118, 416, 729, 750, 1398, 1646
BstV2I GAAGAC 1 cut(s) 1141
BstX2I RGATCY 2 cut(s) 1616, 1680
BstYI RGATCY 2 cut(s) 1616, 1680
Bsu36I CCTNAGG 1 cut(s) 288
BsuI GTATCC 1 cut(s) 1531
BsuRI GGCC 2 cut(s) 1314, 1438
BtgI CCRYGG 4 cut(s) 105, 399, 1439, 1514
BtgZI GCGATG 1 cut(s) 231
BtsCI GGATG 4 cut(s) 101, 1015, 1511, 1515
BtsI GCAGTG 3 cut(s) 826, 1029, 1089
BtsIMutI CAGTG 6 cut(s) 612, 826, 848, 1029, 1089, 1656
Cac8I GCNNGC 5 cut(s) 45, 761, 1316, 1639, 1711
CfoI GCGC 2 cut(s) 811, 1532
Cfr13I GGNCC 3 cut(s) 754, 1097, 1571
CseI GACGC 1 cut(s) 65
CsiI ACCWGGT 1 cut(s) 1573
Csp6I GTAC 3 cut(s) 229, 829, 1465
CviAII CATG 6 cut(s) 106, 1306, 1440, 1468, 1480, 1515
CviQI GTAC 3 cut(s) 229, 829, 1465
DdeI CTNAG 8 cut(s) 177, 254, 288, 515, 615, 680, 1261, 1674
DpnI GATC 6 cut(s) 447, 501, 1140, 1350, 1618, 1682
DpnII GATC 6 cut(s) 445, 499, 1138, 1348, 1616, 1680
DriI GACNNNNNGTC 2 cut(s) 394, 463
Eam1104I CTCTTC 2 cut(s) 526, 872
Eam1105I GACNNNNNGTC 2 cut(s) 394, 463
EarI CTCTTC 2 cut(s) 526, 872
Ecl136II GAGCTC 1 cut(s) 519
Eco130I CCWWGG 5 cut(s) 105, 384, 1439, 1514, 1666
Eco24I GRGCYC 2 cut(s) 185, 521
Eco47I GGWCC 3 cut(s) 754, 1097, 1571
Eco47III AGCGCT 1 cut(s) 1531
Eco53kI GAGCTC 1 cut(s) 519
Eco57I CTGAAG 3 cut(s) 507, 752, 872
Eco81I CCTNAGG 1 cut(s) 288
Eco88I CYCGRG 3 cut(s) 184, 1068, 1430
EcoICRI GAGCTC 1 cut(s) 519
EcoRI GAATTC 1 cut(s) 172
EcoRII CCWGG 7 cut(s) 231, 723, 1110, 1176, 1309, 1412, 1573
EcoT14I CCWWGG 5 cut(s) 105, 384, 1439, 1514, 1666
EcoT38I GRGCYC 2 cut(s) 185, 521
ErhI CCWWGG 5 cut(s) 105, 384, 1439, 1514, 1666
FaeI CATG 6 cut(s) 109, 1309, 1443, 1471, 1483, 1518
FaqI GGGAC 1 cut(s) 1085
FatI CATG 6 cut(s) 105, 1305, 1439, 1467, 1479, 1514
Fnu4HI GCNGC 7 cut(s) 132, 342, 405, 743, 764, 1387, 1635
FokI GGATG 4 cut(s) 88, 1022, 1518, 1522
FriOI GRGCYC 2 cut(s) 185, 521
Fsp4HI GCNGC 7 cut(s) 132, 342, 405, 743, 764, 1387, 1635
FspBI CTAG 2 cut(s) 345, 624
GlaI GCGC 2 cut(s) 810, 1531
GluI GCNGC 7 cut(s) 132, 342, 405, 743, 764, 1387, 1635
GsuI CTGGAG 1 cut(s) 1259
HaeII RGCGCY 1 cut(s) 1533
HaeIII GGCC 2 cut(s) 1314, 1438
HapII CCGG 3 cut(s) 49, 1095, 1535
HgaI GACGC 1 cut(s) 65
HhaI GCGC 2 cut(s) 811, 1532
Hin1II CATG 6 cut(s) 109, 1309, 1443, 1471, 1483, 1518
Hin6I GCGC 2 cut(s) 809, 1530
HinP1I GCGC 2 cut(s) 809, 1530
HincII GTYRAC 4 cut(s) 268, 621, 1131, 1243
HindII GTYRAC 4 cut(s) 268, 621, 1131, 1243
HinfI GANTC 5 cut(s) 35, 236, 433, 1059, 1447
HpaI GTTAAC 2 cut(s) 268, 621
HpaII CCGG 3 cut(s) 49, 1095, 1535
HphI GGTGA 2 cut(s) 685, 974
Hpy166II GTNNAC 5 cut(s) 268, 621, 1131, 1243, 1465
Hpy188III TCNNGA 8 cut(s) 422, 505, 842, 1070, 1255, 1276, 1432, 1535
Hpy8I GTNNAC 5 cut(s) 268, 621, 1131, 1243, 1465
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 9 cut(s) 19, 545, 745, 784, 847, 895, 1047, 1408, 1600
HpyCH4III ACNGT 5 cut(s) 151, 632, 1240, 1462, 1651
HpyCH4IV ACGT 2 cut(s) 894, 1002
HpyCH4V TGCA 9 cut(s) 546, 745, 848, 977, 1224, 1284, 1386, 1471, 1654
HpyF10VI GCNNNNNNNGC 8 cut(s) 49, 292, 525, 739, 854, 1040, 1100, 1315
HpyF3I CTNAG 8 cut(s) 177, 254, 288, 515, 615, 680, 1261, 1674
HpySE526I ACGT 2 cut(s) 894, 1002
Hsp92II CATG 6 cut(s) 109, 1309, 1443, 1471, 1483, 1518
HspAI GCGC 2 cut(s) 809, 1530
Kpn2I TCCGGA 1 cut(s) 1534
KspAI GTTAAC 2 cut(s) 268, 621
Kzo9I GATC 6 cut(s) 445, 499, 1138, 1348, 1616, 1680
LguI GCTCTTC 1 cut(s) 526
LmnI GCTCC 3 cut(s) 292, 1278, 1537
Lsp1109I GCAGC 6 cut(s) 118, 416, 729, 750, 1398, 1646
LweI GCATC 7 cut(s) 198, 460, 754, 835, 1159, 1500, 1678
MabI ACCWGGT 1 cut(s) 1573
MaeI CTAG 2 cut(s) 345, 624
MaeII ACGT 2 cut(s) 894, 1002
MaeIII GTNAC 3 cut(s) 220, 242, 1696
MalI GATC 6 cut(s) 447, 501, 1140, 1350, 1618, 1682
MboI GATC 6 cut(s) 445, 499, 1138, 1348, 1616, 1680
MboII GAAGA 8 cut(s) 513, 616, 799, 889, 910, 1141, 1456, 1572
MfeI CAATTG 1 cut(s) 1381
MflI RGATCY 2 cut(s) 1616, 1680
MhlI GDGCHC 4 cut(s) 185, 521, 1275, 1526
MlyI GAGTC 2 cut(s) 230, 442
MmeI TCCRAC 3 cut(s) 44, 121, 297
MnlI CCTC 6 cut(s) 270, 283, 777, 1428, 1439, 1499
MroI TCCGGA 1 cut(s) 1534
MseI TTAA 6 cut(s) 267, 282, 372, 620, 666, 1599
MslI CAYNNNNRTG 4 cut(s) 803, 893, 1167, 1460
MspI CCGG 3 cut(s) 49, 1095, 1535
MspR9I CCNGG 8 cut(s) 49, 233, 725, 1112, 1178, 1311, 1414, 1575
MunI CAATTG 1 cut(s) 1381
Mva1269I GAATGC 2 cut(s) 1226, 1654
MvaI CCWGG 7 cut(s) 233, 725, 1112, 1178, 1311, 1414, 1575
MvnI CGCG 2 cut(s) 811, 1218
MwoI GCNNNNNNNGC 8 cut(s) 49, 292, 525, 739, 854, 1040, 1100, 1315
NciI CCSGG 1 cut(s) 49
NcoI CCATGG 3 cut(s) 105, 1439, 1514
NdeII GATC 6 cut(s) 445, 499, 1138, 1348, 1616, 1680
NlaIII CATG 6 cut(s) 109, 1309, 1443, 1471, 1483, 1518
NlaIV GGNNCC 2 cut(s) 145, 1160
NmuCI GTSAC 1 cut(s) 220
NspI RCATGY 1 cut(s) 1471
OliI CACNNNNGTG 1 cut(s) 1460
PciSI GCTCTTC 1 cut(s) 526
PcsI WCGNNNNNNNCGW 1 cut(s) 70
PctI GAATGC 2 cut(s) 1226, 1654
PfeI GAWTC 3 cut(s) 35, 1059, 1447
PflFI GACNNNGTC 1 cut(s) 800
PfoI TCCNGGA 1 cut(s) 1176
PkrI GCNGC 7 cut(s) 133, 343, 406, 744, 765, 1388, 1636
PleI GAGTC 2 cut(s) 230, 441
PpsI GAGTC 2 cut(s) 230, 441
Ppu21I YACGTR 1 cut(s) 895
PshAI GACNNNNGTC 1 cut(s) 60
Psp124BI GAGCTC 1 cut(s) 521
Psp1406I AACGTT 1 cut(s) 1002
Psp6I CCWGG 7 cut(s) 231, 723, 1110, 1176, 1309, 1412, 1573
PspGI CCWGG 7 cut(s) 231, 723, 1110, 1176, 1309, 1412, 1573
PspN4I GGNNCC 2 cut(s) 145, 1160
PspPI GGNCC 3 cut(s) 754, 1097, 1571
PsuI RGATCY 2 cut(s) 1616, 1680
PsyI GACNNNGTC 1 cut(s) 800
RsaI GTAC 3 cut(s) 230, 830, 1466
RsaNI GTAC 3 cut(s) 229, 829, 1465
RseI CAYNNNNRTG 4 cut(s) 803, 893, 1167, 1460
SacI GAGCTC 1 cut(s) 521
SapI GCTCTTC 1 cut(s) 526
SaqAI TTAA 6 cut(s) 267, 282, 372, 620, 666, 1599
SatI GCNGC 7 cut(s) 132, 342, 405, 743, 764, 1387, 1635
Sau3AI GATC 6 cut(s) 445, 499, 1138, 1348, 1616, 1680
Sau96I GGNCC 3 cut(s) 754, 1097, 1571
SchI GAGTC 2 cut(s) 230, 442
ScrFI CCNGG 8 cut(s) 49, 233, 725, 1112, 1178, 1311, 1414, 1575
SduI GDGCHC 4 cut(s) 185, 521, 1275, 1526
SexAI ACCWGGT 1 cut(s) 1573
SfaNI GCATC 7 cut(s) 198, 460, 754, 835, 1159, 1500, 1678
SfcI CTRYAG 2 cut(s) 718, 1153
SinI GGWCC 3 cut(s) 754, 1097, 1571
SmiMI CAYNNNNRTG 4 cut(s) 803, 893, 1167, 1460
SmlI CTYRAG 2 cut(s) 578, 1335
SmoI CTYRAG 2 cut(s) 578, 1335
SsiI CCGC 4 cut(s) 342, 757, 1134, 1216
SspMI CTAG 2 cut(s) 345, 624
SstI GAGCTC 1 cut(s) 521
StyD4I CCNGG 8 cut(s) 47, 231, 723, 1110, 1176, 1309, 1412, 1573
StyI CCWWGG 5 cut(s) 105, 384, 1439, 1514, 1666
TaaI ACNGT 5 cut(s) 151, 632, 1240, 1462, 1651
TaiI ACGT 2 cut(s) 897, 1005
TaqI TCGA 3 cut(s) 54, 73, 971
TatI WGTACW 2 cut(s) 828, 1464
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 3 cut(s) 35, 1059, 1447
Tru1I TTAA 6 cut(s) 267, 282, 372, 620, 666, 1599
Tru9I TTAA 6 cut(s) 267, 282, 372, 620, 666, 1599
TscAI CASTG 6 cut(s) 619, 826, 855, 1036, 1096, 1656
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 6 cut(s) 131, 404, 742, 763, 1386, 1634
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 7 cut(s) 48, 143, 342, 576, 1051, 1237, 1647
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 6 cut(s) 619, 826, 855, 1036, 1096, 1656
Tth111I GACNNNGTC 1 cut(s) 800
VpaK11BI GGWCC 3 cut(s) 754, 1097, 1571
XapI RAATTY 5 cut(s) 172, 1368, 1556, 1564, 1643
XceI RCATGY 1 cut(s) 1471
XspI CTAG 2 cut(s) 345, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.