RLG00000029761

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0013785
Physical Location & Seq
Forward (+)
48726568 .. 48728701
2134 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000029761

Sequence Viewer

Length: 1716 bp
ATGTCTTCCTTCAAAGCTTATGGCAAAGTCAGTGAATCCGACCAAGCCAAGCTTGATGCTCGTCGCAAGACTCGCAGGAGAGTCGCCATCATATCTATTTCCTCCATAGTTCTTGTCTGCGTCGTGGTTGCCGCCGTGGTTGGAACCGCTACTCATAACTCCAAAAAAGGTGATGACAATGGCAAAGGTACGGTTTCCGCTTCCGTCAAAGCCGTTTGTGACGTGACATTGTACCAGGACTCGTGTTATTCTAGTCTTGGTCCTATTGTCAACTCCAGCAATTTTAAGCCTGAGGATTTGTTTAAGTTGTCAATTCAAGTGGCAATTACAGAACTGTCCAAAGCTGCTACTCAAATTTCTGATCAAGGGCTCTTCAAAGGTACTGTCACAGACAAATTGTCCGTGGCAGCTTTGAAAAACTGCGGCGAGCTTCTGAGTCTTGCCTTGGATCATCTCAACGAGTCGTTGTCCGGGGATGGTTCCTTGCTTGAAGTGGTTGAAGATCTTAAAACATGGTTGAGCTCTGCAGGAACTTACCAGCAGTCATGTGTAGATGGGTTTGAGGAAGCAAAAGGAGAGCTAAAGGCTACCATTGAAAAGTATCTCAAGACTTCTACTGAACTAACCAGTAACAGCCTTGCCATTGTCTCTTGGATTTCGAAAGTCGCCAACTCTGTGAAGCTAAGGCGGTTGCTGAGTCATGATGGTGCCGCGGCTAGCCATGGCATGCCGGAGTGGCTGCACCAGACGGACCGCAGGCTAATTGAAAGTTCGGATTTGAGGAAGAAGGCCAACATTGTTGTGGCCAAAGATGGGAGTGGCAAATACAAGACAATAGGTGCTGCACTGAAGGCTGTGCCGGACAAAAGCAAGAAGACAACAGTCATCTACGTGAAGAACGGTGTTTATTTTGAGAATGTTCGAGTGGAGAAATCCAAGTGGAATGTTGTGGTGGTCGGCGATGGAATGACTTCAACTATTGTGTCTGCCAGCCTTAACGTTGTGGATGGAACTCCCACGTTTTCCAGTGCAACATTCGCGGTTTTTGGGAAGGGATTCGTTGCCAGAGACATTGGATTTCGTAACACAGCCGGAGCAGCCAAGCACCAGGCGGTGGCACTAATGTCGAGTGCTGACCAGACTCTGTTCTACAGATGCCACATTGACGCGTTCCAGGACTCGCTCTACGTTCATGCCAATCGCCAATTCTACCGAGAATGCAACATTTACGGAACAGTTGATTTCATTTTCGGAAACTCAGCAGTAGTCCTCCAAAACTGTAAGGTGTTCCCCAAGGTACCAATGGTTGGCCAGCAGAACACAATCACGGCACAAGGCAAAATCGACCCAAACCAAAACACGGGAATCGCATTTCAGAACTGCACCATTTCGCCTTTTGGAGACTTGTCTTCGGTTAAGACTTACCTCGGGAGACCTTGGAAGAATTACTCCACAACAGTGTACATGCAAACATTCATGGGAAGCTTGATTCACCCAGATGGATGGTTGCCATGGGTAGGCACAACGGCACCTGACACCATTTTTTACTCCGAGTTTCAGAATTTCGGACCTGGTTCTGTGACAAAGAATAGAGTTAAGTGGAAGGGCTTAAAAACAATAACCAGCAAGGTGGCCGACAAATTTACCGTGCACTCATTTCTTCAAGGAACCAAGTGGATCTCCGATGCTAGTGTTCCCTATAAATCCAACCTCTGA

Protein Analysis

572

Amino Acids

62.1

Weight (kDa)

9.27

Isoelectric Point (pI)

28.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 68 - 215 5.9e-32 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 265 - 556 3.9e-135 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0013785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10710 AT5G04960
fragaria_vesca FvH4_7g04230 FvH4_7g04920 FvH4_7g04920
malus_domestica MD02G1266400.v1.1 MD07G1053300.v1.1
prunus_persica Prupe.2G058300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0331001
rosa_laevigata RLG00000029761
rosa_roxburghii Rroxscaffold_4G00319560
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113100 Rh1BG087100 Rh1CG110000 Rh1DG118800
rosa_wichuraiana Rw1G009220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1297
AccB1I GGYRCC 3 cut(s) 707, 1297, 1528
AccB7I CCANNNNNTGG 2 cut(s) 1114, 1307
AccII CGCG 3 cut(s) 713, 1040, 1169
AclI AACGTT 1 cut(s) 999
AclWI GGATC 2 cut(s) 456, 1685
AcoI YGGCCR 3 cut(s) 804, 1309, 1632
AcsI RAATTY 3 cut(s) 354, 1561, 1640
AcuI CTGAAG 1 cut(s) 869
AfaI GTAC 5 cut(s) 190, 233, 382, 1299, 1463
AfiI CCNNNNNNNGG 5 cut(s) 813, 1114, 1307, 1360, 1517
AflIII ACRYGT 1 cut(s) 1167
AhdI GACNNNNNGTC 1 cut(s) 397
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 4 cut(s) 234, 1107, 1173, 1570
AjuI GAANNNNNNNTTGG 2 cut(s) 1190, 1222
AleI CACNNNNGTG 1 cut(s) 1457
AluBI AGCT 9 cut(s) 17, 52, 344, 410, 430, 522, 580, 682, 1485
AluI AGCT 9 cut(s) 17, 52, 344, 410, 430, 522, 580, 682, 1485
Alw21I GWGCWC 2 cut(s) 524, 1653
Alw26I GTCTC 4 cut(s) 652, 1062, 1395, 1426
Alw44I GTGCAC 1 cut(s) 1649
AlwI GGATC 2 cut(s) 456, 1685
AlwNI CAGNNNCTG 1 cut(s) 1144
Ama87I CYCGRG 1 cut(s) 1427
AoxI GGCC 4 cut(s) 789, 804, 1309, 1632
ApaLI GTGCAC 1 cut(s) 1649
ApeKI GCWGC 5 cut(s) 344, 407, 739, 842, 1097
ApoI RAATTY 3 cut(s) 354, 1561, 1640
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
Asp700I GAANNNNTTC 2 cut(s) 970, 1055
Asp718I GGTACC 1 cut(s) 1297
AspS9I GGNCC 3 cut(s) 260, 751, 1568
AsuC2I CCSGG 1 cut(s) 472
AsuHPI GGTGA 2 cut(s) 182, 1484
AsuII TTCGAA 1 cut(s) 659
AsuNHI GCTAGC 1 cut(s) 716
AvaI CYCGRG 1 cut(s) 1427
AvaII GGWCC 3 cut(s) 260, 751, 1568
AxyI CCTNAGG 1 cut(s) 291
BaeGI GKGCMC 1 cut(s) 1653
BalI TGGCCA 2 cut(s) 806, 1311
BanI GGYRCC 3 cut(s) 707, 1297, 1528
BanII GRGCYC 2 cut(s) 372, 524
BauI CACGAG 1 cut(s) 241
BbsI GAAGAC 2 cut(s) 881, 1401
Bbv12I GWGCWC 2 cut(s) 524, 1653
BbvI GCAGC 5 cut(s) 331, 419, 726, 829, 1109
BccI CCATC 9 cut(s) 95, 470, 548, 698, 806, 956, 1001, 1493, 1497
BceAI ACGGC 4 cut(s) 119, 197, 1344, 1542
BcgI CGANNNNNNTGC 4 cut(s) 64, 98, 1107, 1141
BciT130I CCWGG 4 cut(s) 236, 1109, 1175, 1572
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 1 cut(s) 472
BcoDI GTCTC 4 cut(s) 652, 1062, 1395, 1426
BfaI CTAG 3 cut(s) 252, 717, 1689
BfmI CTRYAG 2 cut(s) 525, 1150
BglI GCCNNNNNGGC 1 cut(s) 736
BglII AGATCT 1 cut(s) 502
BisI GCNGC 9 cut(s) 132, 345, 408, 424, 711, 714, 740, 843, 1098
BlsI GCNGC 9 cut(s) 133, 346, 409, 425, 712, 715, 741, 844, 1099
Bme1390I CCNGG 5 cut(s) 236, 472, 1109, 1175, 1572
Bme18I GGWCC 3 cut(s) 260, 751, 1568
BmeRI GACNNNNNGTC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 1427
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 3 cut(s) 260, 751, 1568
BmiI GGNNCC 6 cut(s) 145, 481, 709, 1299, 1530, 1669
BmrFI CCNGG 5 cut(s) 236, 472, 1109, 1175, 1572
BmsI GCATC 3 cut(s) 46, 1145, 1675
BmtI GCTAGC 1 cut(s) 720
BoxI GACNNNNGTC 1 cut(s) 881
BpiI GAAGAC 2 cut(s) 881, 1401
BpmI CTGGAG 1 cut(s) 259
Bpu10I CCTNAGC 1 cut(s) 683
Bpu14I TTCGAA 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 590
BpuMI CCSGG 1 cut(s) 472
BsaAI YACGTR 1 cut(s) 892
BsaI GGTCTC 1 cut(s) 1426
Bsc4I CCNNNNNNNGG 5 cut(s) 813, 1114, 1307, 1360, 1517
Bse1I ACTGG 2 cut(s) 627, 1026
Bse21I CCTNAGG 1 cut(s) 291
BseBI CCWGG 4 cut(s) 236, 1109, 1175, 1572
BseGI GGATG 3 cut(s) 481, 1012, 1508
BseLI CCNNNNNNNGG 5 cut(s) 813, 1114, 1307, 1360, 1517
BseMII CTCAG 4 cut(s) 282, 425, 686, 1272
BseNI ACTGG 2 cut(s) 627, 1026
BseSI GKGCMC 1 cut(s) 1653
BseXI GCAGC 5 cut(s) 331, 419, 726, 829, 1109
BsgI GTGCAG 3 cut(s) 725, 828, 1366
Bsh1236I CGCG 3 cut(s) 713, 1040, 1169
BshFI GGCC 4 cut(s) 791, 806, 1311, 1634
BshNI GGYRCC 3 cut(s) 707, 1297, 1528
BsiHKAI GWGCWC 2 cut(s) 524, 1653
BsiHKCI CYCGRG 1 cut(s) 1427
BsiSI CCGG 4 cut(s) 471, 731, 860, 1092
BslI CCNNNNNNNGG 5 cut(s) 813, 1114, 1307, 1360, 1517
BsmAI GTCTC 4 cut(s) 652, 1062, 1395, 1426
BsmI GAATGC 1 cut(s) 1223
BsnI GGCC 4 cut(s) 791, 806, 1311, 1634
Bso31I GGTCTC 1 cut(s) 1426
BsoBI CYCGRG 1 cut(s) 1427
Bsp119I TTCGAA 1 cut(s) 659
Bsp1286I GDGCHC 3 cut(s) 372, 524, 1653
Bsp1407I TGTACA 1 cut(s) 1461
Bsp143I GATC 4 cut(s) 361, 448, 502, 1677
Bsp19I CCATGG 2 cut(s) 721, 1511
BspANI GGCC 4 cut(s) 791, 806, 1311, 1634
BspCNI CTCAG 4 cut(s) 283, 426, 687, 1271
BspFNI CGCG 3 cut(s) 713, 1040, 1169
BspHI TCATGA 1 cut(s) 700
BspLI GGNNCC 6 cut(s) 145, 481, 709, 1299, 1530, 1669
BspMAI CTGCAG 1 cut(s) 529
BspOI GCTAGC 1 cut(s) 720
BspPI GGATC 2 cut(s) 456, 1685
BspQI GCTCTTC 1 cut(s) 377
BspT104I TTCGAA 1 cut(s) 659
BspT107I GGYRCC 3 cut(s) 707, 1297, 1528
BspTNI GGTCTC 1 cut(s) 1426
BsrGI TGTACA 1 cut(s) 1461
BsrI ACTGG 2 cut(s) 627, 1026
BssMI GATC 4 cut(s) 361, 448, 502, 1677
BssSI CACGAG 1 cut(s) 241
BssT1I CCWWGG 5 cut(s) 444, 721, 1293, 1436, 1511
Bst2BI CACGAG 1 cut(s) 241
Bst2UI CCWGG 4 cut(s) 236, 1109, 1175, 1572
Bst4CI ACNGT 9 cut(s) 193, 336, 385, 883, 902, 1237, 1280, 1459, 1648
Bst6I CTCTTC 1 cut(s) 377
BstAUI TGTACA 1 cut(s) 1461
BstBAI YACGTR 1 cut(s) 892
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 6 cut(s) 428, 718, 728, 758, 991, 1313
BstDEI CTNAG 5 cut(s) 291, 434, 683, 695, 1258
BstDSI CCRYGG 5 cut(s) 135, 402, 711, 721, 1511
BstF5I GGATG 3 cut(s) 481, 1012, 1508
BstFNI CGCG 3 cut(s) 713, 1040, 1169
BstKTI GATC 4 cut(s) 364, 451, 505, 1680
BstMAI GTCTC 4 cut(s) 652, 1062, 1395, 1426
BstMBI GATC 4 cut(s) 361, 448, 502, 1677
BstMWI GCNNNNNNNGC 5 cut(s) 72, 736, 851, 1037, 1097
BstNI CCWGG 4 cut(s) 236, 1109, 1175, 1572
BstNSI RCATGY 2 cut(s) 730, 1468
BstPAI GACNNNNGTC 1 cut(s) 881
BstSCI CCNGG 5 cut(s) 234, 470, 1107, 1173, 1570
BstSFI CTRYAG 2 cut(s) 525, 1150
BstSLI GKGCMC 1 cut(s) 1653
BstUI CGCG 3 cut(s) 713, 1040, 1169
BstV1I GCAGC 5 cut(s) 331, 419, 726, 829, 1109
BstV2I GAAGAC 2 cut(s) 881, 1401
BstX2I RGATCY 2 cut(s) 502, 1677
BstXI CCANNNNNNTGG 2 cut(s) 1503, 1630
BstYI RGATCY 2 cut(s) 502, 1677
Bsu36I CCTNAGG 1 cut(s) 291
BsuRI GGCC 4 cut(s) 791, 806, 1311, 1634
BtgI CCRYGG 5 cut(s) 135, 402, 711, 721, 1511
BtgZI GCGATG 1 cut(s) 975
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 3 cut(s) 481, 1012, 1508
BtsIMutI CAGTG 4 cut(s) 37, 845, 1033, 1464
Cac8I GCNNGC 6 cut(s) 428, 718, 728, 758, 991, 1313
CaiI CAGNNNCTG 1 cut(s) 1144
CciI TCATGA 1 cut(s) 700
Cfr13I GGNCC 3 cut(s) 260, 751, 1568
Cfr42I CCGCGG 1 cut(s) 714
CpoI CGGWCCG 1 cut(s) 751
CseI GACGC 2 cut(s) 109, 1175
CsiI ACCWGGT 1 cut(s) 1570
Csp6I GTAC 5 cut(s) 189, 232, 381, 1298, 1462
CspCI CAANNNNNGTGG 2 cut(s) 300, 335
CspI CGGWCCG 1 cut(s) 751
CviAII CATG 9 cut(s) 513, 546, 701, 722, 727, 1193, 1465, 1477, 1512
CviQI GTAC 5 cut(s) 189, 232, 381, 1298, 1462
DdeI CTNAG 5 cut(s) 291, 434, 683, 695, 1258
DpnI GATC 4 cut(s) 363, 450, 504, 1679
DpnII GATC 4 cut(s) 361, 448, 502, 1677
DriI GACNNNNNGTC 1 cut(s) 397
EaeI YGGCCR 3 cut(s) 804, 1309, 1632
Eam1104I CTCTTC 1 cut(s) 377
Eam1105I GACNNNNNGTC 1 cut(s) 397
EarI CTCTTC 1 cut(s) 377
Ecl136II GAGCTC 1 cut(s) 522
Eco130I CCWWGG 5 cut(s) 444, 721, 1293, 1436, 1511
Eco24I GRGCYC 2 cut(s) 372, 524
Eco31I GGTCTC 1 cut(s) 1426
Eco47I GGWCC 3 cut(s) 260, 751, 1568
Eco53kI GAGCTC 1 cut(s) 522
Eco57I CTGAAG 1 cut(s) 869
Eco81I CCTNAGG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 1427
EcoICRI GAGCTC 1 cut(s) 522
EcoRII CCWGG 4 cut(s) 234, 1107, 1173, 1570
EcoT14I CCWWGG 5 cut(s) 444, 721, 1293, 1436, 1511
EcoT38I GRGCYC 2 cut(s) 372, 524
ErhI CCWWGG 5 cut(s) 444, 721, 1293, 1436, 1511
FaeI CATG 9 cut(s) 516, 549, 704, 725, 730, 1196, 1468, 1480, 1515
FalI AAGNNNNNCTT 2 cut(s) 36, 68
FatI CATG 9 cut(s) 512, 545, 700, 721, 726, 1192, 1464, 1476, 1511
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 9 cut(s) 132, 345, 408, 424, 711, 714, 740, 843, 1098
FokI GGATG 3 cut(s) 488, 1019, 1515
FriOI GRGCYC 2 cut(s) 372, 524
Fsp4HI GCNGC 9 cut(s) 132, 345, 408, 424, 711, 714, 740, 843, 1098
FspBI CTAG 3 cut(s) 252, 717, 1689
GluI GCNGC 9 cut(s) 132, 345, 408, 424, 711, 714, 740, 843, 1098
GsuI CTGGAG 1 cut(s) 259
HaeIII GGCC 4 cut(s) 791, 806, 1311, 1634
HapII CCGG 4 cut(s) 471, 731, 860, 1092
HgaI GACGC 2 cut(s) 109, 1175
Hin1II CATG 9 cut(s) 516, 549, 704, 725, 730, 1196, 1468, 1480, 1515
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 3 cut(s) 15, 50, 1483
HpaII CCGG 4 cut(s) 471, 731, 860, 1092
HphI GGTGA 2 cut(s) 182, 1484
Hpy166II GTNNAC 3 cut(s) 271, 1462, 1651
Hpy188III TCNNGA 3 cut(s) 607, 701, 1429
Hpy8I GTNNAC 3 cut(s) 271, 1462, 1651
Hpy99I CGWCG 2 cut(s) 66, 125
HpyAV CCTTC 5 cut(s) 19, 781, 844, 1045, 1597
HpyCH4III ACNGT 9 cut(s) 193, 336, 385, 883, 902, 1237, 1280, 1459, 1648
HpyCH4IV ACGT 5 cut(s) 222, 891, 999, 1019, 1188
HpyCH4V TGCA 8 cut(s) 527, 742, 845, 1031, 1221, 1383, 1468, 1651
HpyF10VI GCNNNNNNNGC 5 cut(s) 72, 736, 851, 1037, 1097
HpyF3I CTNAG 5 cut(s) 291, 434, 683, 695, 1258
HpySE526I ACGT 5 cut(s) 222, 891, 999, 1019, 1188
Hsp92II CATG 9 cut(s) 516, 549, 704, 725, 730, 1196, 1468, 1480, 1515
KpnI GGTACC 1 cut(s) 1301
Ksp22I TGATCA 1 cut(s) 361
KspI CCGCGG 1 cut(s) 714
Kzo9I GATC 4 cut(s) 361, 448, 502, 1677
LguI GCTCTTC 1 cut(s) 377
LmnI GCTCC 1 cut(s) 1094
Lsp1109I GCAGC 5 cut(s) 331, 419, 726, 829, 1109
LweI GCATC 3 cut(s) 46, 1145, 1675
MabI ACCWGGT 1 cut(s) 1570
MaeI CTAG 3 cut(s) 252, 717, 1689
MaeII ACGT 5 cut(s) 222, 891, 999, 1019, 1188
MaeIII GTNAC 6 cut(s) 218, 223, 385, 629, 1082, 1579
MalI GATC 4 cut(s) 363, 450, 504, 1679
MboI GATC 4 cut(s) 361, 448, 502, 1677
MboII GAAGA 8 cut(s) 364, 512, 796, 886, 907, 1401, 1453, 1652
MflI RGATCY 2 cut(s) 502, 1677
MhlI GDGCHC 3 cut(s) 372, 524, 1653
MlsI TGGCCA 2 cut(s) 806, 1311
MluI ACGCGT 1 cut(s) 1167
MluNI TGGCCA 2 cut(s) 806, 1311
MlyI GAGTC 8 cut(s) 64, 90, 233, 445, 470, 706, 1135, 1172
MmeI TCCRAC 2 cut(s) 63, 121
MnlI CCTC 6 cut(s) 112, 286, 556, 774, 1280, 1436
Mox20I TGGCCA 2 cut(s) 806, 1311
MroXI GAANNNNTTC 2 cut(s) 970, 1055
MscI TGGCCA 2 cut(s) 806, 1311
MseI TTAA 7 cut(s) 285, 303, 507, 996, 1416, 1596, 1610
MslI CAYNNNNRTG 5 cut(s) 705, 800, 890, 1457, 1497
Msp20I TGGCCA 2 cut(s) 806, 1311
MspA1I CMGCKG 1 cut(s) 713
MspI CCGG 4 cut(s) 471, 731, 860, 1092
MspR9I CCNGG 5 cut(s) 236, 472, 1109, 1175, 1572
Mva1269I GAATGC 1 cut(s) 1223
MvaI CCWGG 4 cut(s) 236, 1109, 1175, 1572
MvnI CGCG 3 cut(s) 713, 1040, 1169
MwoI GCNNNNNNNGC 5 cut(s) 72, 736, 851, 1037, 1097
NciI CCSGG 1 cut(s) 472
NcoI CCATGG 2 cut(s) 721, 1511
NdeII GATC 4 cut(s) 361, 448, 502, 1677
NheI GCTAGC 1 cut(s) 716
NlaIII CATG 9 cut(s) 516, 549, 704, 725, 730, 1196, 1468, 1480, 1515
NlaIV GGNNCC 6 cut(s) 145, 481, 709, 1299, 1530, 1669
NmuCI GTSAC 4 cut(s) 218, 223, 385, 1579
NspI RCATGY 2 cut(s) 730, 1468
NspV TTCGAA 1 cut(s) 659
OliI CACNNNNGTG 1 cut(s) 1457
PaeI GCATGC 1 cut(s) 730
PagI TCATGA 1 cut(s) 700
PciSI GCTCTTC 1 cut(s) 377
PctI GAATGC 1 cut(s) 1223
PdmI GAANNNNTTC 2 cut(s) 970, 1055
PfeI GAWTC 4 cut(s) 35, 1056, 1365, 1489
PflMI CCANNNNNTGG 2 cut(s) 1114, 1307
PfoI TCCNGGA 1 cut(s) 1173
PkrI GCNGC 9 cut(s) 133, 346, 409, 425, 712, 715, 741, 844, 1099
PleI GAGTC 8 cut(s) 64, 89, 233, 444, 469, 705, 1135, 1172
PpsI GAGTC 8 cut(s) 64, 89, 233, 444, 469, 705, 1135, 1172
Ppu21I YACGTR 1 cut(s) 892
PshAI GACNNNNGTC 1 cut(s) 881
Psp124BI GAGCTC 1 cut(s) 524
Psp1406I AACGTT 1 cut(s) 999
Psp6I CCWGG 4 cut(s) 234, 1107, 1173, 1570
PspGI CCWGG 4 cut(s) 234, 1107, 1173, 1570
PspN4I GGNNCC 6 cut(s) 145, 481, 709, 1299, 1530, 1669
PspPI GGNCC 3 cut(s) 260, 751, 1568
PstI CTGCAG 1 cut(s) 529
PstNI CAGNNNCTG 1 cut(s) 1144
PsuI RGATCY 2 cut(s) 502, 1677
RsaI GTAC 5 cut(s) 190, 233, 382, 1299, 1463
RsaNI GTAC 5 cut(s) 189, 232, 381, 1298, 1462
RseI CAYNNNNRTG 5 cut(s) 705, 800, 890, 1457, 1497
Rsr2I CGGWCCG 1 cut(s) 751
RsrII CGGWCCG 1 cut(s) 751
SacI GAGCTC 1 cut(s) 524
SacII CCGCGG 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 377
SaqAI TTAA 7 cut(s) 285, 303, 507, 996, 1416, 1596, 1610
SatI GCNGC 9 cut(s) 132, 345, 408, 424, 711, 714, 740, 843, 1098
Sau3AI GATC 4 cut(s) 361, 448, 502, 1677
Sau96I GGNCC 3 cut(s) 260, 751, 1568
SchI GAGTC 8 cut(s) 64, 90, 233, 445, 470, 706, 1135, 1172
ScrFI CCNGG 5 cut(s) 236, 472, 1109, 1175, 1572
SduI GDGCHC 3 cut(s) 372, 524, 1653
SexAI ACCWGGT 1 cut(s) 1570
SfaNI GCATC 3 cut(s) 46, 1145, 1675
SfcI CTRYAG 2 cut(s) 525, 1150
Sfr303I CCGCGG 1 cut(s) 714
SfuI TTCGAA 1 cut(s) 659
SgrBI CCGCGG 1 cut(s) 714
SinI GGWCC 3 cut(s) 260, 751, 1568
SmiMI CAYNNNNRTG 5 cut(s) 705, 800, 890, 1457, 1497
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
SphI GCATGC 1 cut(s) 730
SspMI CTAG 3 cut(s) 252, 717, 1689
SstI GAGCTC 1 cut(s) 524
StyD4I CCNGG 5 cut(s) 234, 470, 1107, 1173, 1570
StyI CCWWGG 5 cut(s) 444, 721, 1293, 1436, 1511
TaaI ACNGT 9 cut(s) 193, 336, 385, 883, 902, 1237, 1280, 1459, 1648
TaiI ACGT 5 cut(s) 225, 894, 1002, 1022, 1191
TaqI TCGA 4 cut(s) 659, 922, 1127, 1344
TatI WGTACW 1 cut(s) 1461
TauI GCSGC 4 cut(s) 134, 426, 713, 716
TfiI GAWTC 4 cut(s) 35, 1056, 1365, 1489
Tru1I TTAA 7 cut(s) 285, 303, 507, 996, 1416, 1596, 1610
Tru9I TTAA 7 cut(s) 285, 303, 507, 996, 1416, 1596, 1610
TscAI CASTG 4 cut(s) 37, 852, 1033, 1464
TseFI GTSAC 4 cut(s) 218, 223, 385, 1579
TseI GCWGC 5 cut(s) 344, 407, 739, 842, 1097
Tsp45I GTSAC 4 cut(s) 218, 223, 385, 1579
TspDTI ATGAA 3 cut(s) 1181, 1234, 1465
TspGWI ACGGA 4 cut(s) 193, 391, 764, 1245
TspRI CASTG 4 cut(s) 37, 852, 1033, 1464
Van91I CCANNNNNTGG 2 cut(s) 1114, 1307
VneI GTGCAC 1 cut(s) 1649
VpaK11BI GGWCC 3 cut(s) 260, 751, 1568
XapI RAATTY 3 cut(s) 354, 1561, 1640
XceI RCATGY 2 cut(s) 730, 1468
XcmI CCANNNNNNNNNTGG 2 cut(s) 799, 1300
XmnI GAANNNNTTC 2 cut(s) 970, 1055
XspI CTAG 3 cut(s) 252, 717, 1689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.