MD02G1266400.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0013785
Physical Location & Seq
Forward (+)
32053995 .. 32056082
2088 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1266400.v1.1.491

Sequence Viewer

Length: 1719 bp
ATGTCTTCCATCAAATCCTATGGCAAGATTGATGAATCCGACCAAGCCCGGCTCGACGCTCGTAGGGAAACTCGAAAGAGAGTTGCCATCATAAGCCTTTCGTCCATAGTTCTTGTCTGTGTCATTGTTGCTGCTGTGGTTGGAACCACCGTTCATAATAAATCTGGTGGGAACTCTAAAGGCTCGGCCATCTCCACTTCTGTCAAGGCTGTTTGTGATGTGACCTTGTACCAGGATTCGTGTTACAGCAGTCTTGGTCCTCTGGTTAACTCCAACAATGTTAAGCCTGAGGAGATTTTCAAGTTGGCAATTCAGGTGGCGATCAATGAATTGTCGAAAGCCGCTACTCATTTTTCGGCTGATGGTGTTCTTAATGCGACTGCCAAGGACACACAGTCTGTGGCAGCTTTGAAAGATTGTCAAGACCTTTTGAGTCTGGCATTGGATCATCTCAACGACTCATTGTCTGACACTAAGTCGTTGGTTGATGTGTTTGATGATCTCAAGACTTGGCTGAGCTCTTCAGGCACATATCAGCAGACTTGCATTGAAGGTTTTGACGATGTAAAAGGAGAACTCAAGGATAAAGTTGTCGGCTACTTGAAGAACTCCAATGAGTTAACTAGCAACAGCCTCGCCATTGTCTCTTGGATTTCCAAAGTCCTTAACTTAGTGAACCTAAGGCGACTTCTGACACACCCTGATCCCACCGATACGTACAGCCAGGGAGTACCAGAGTGGCTGCATCGGAAGGATCGCAAGCTGCTTCAGAGTTCGGATTTGAGGAAGAAGGCAGACATTGTCGTGGCAAGCAATGGCAGTAGCAAATACAAAACAATTCAGGATGCACTCAATGCTGTTCCGGATAAGAGCAAGAAGAGAACAGTCATCTACGTCAAGAAGGGGGTTTATTATGGGAATGTGAGGGTTGAGAAATCCAAGTGGAATGTTTTGGTGGTCGGTGATGGTTCGAATGCAACTATTGTAACTGCCAAACTCAACGTTGTGGATGGAACTCCCACATTTTCCACTGCCACAGTCGCCGTTTTTGGAAAGGGATTCATTGCCCGGGACATTGGATTTCGCAACACTGCAGGGCCAGCCAAGCACCAGGCAGTGGCCTTGTTGTCAACTGCTGATCAGTCAGTCTTCTATAGGTGCGACATTGATGCATTCCAGGACACTCTCTATGCTCACTCCAATCGCCAATTCTACCGCGAATGCAACATTTATGGAACAGTGGATTTCATTTTCGGGAACTCAGCAGTTGTGCTCCAAAACTGCAACATAATGCCTAAGGTTCCCATGCGAGGCCAGCAGAACACCATCACAGCTCAAGGGAAGTTTGATCCCAATCAAAATACAGGAATTTCGATTCACAATTGCAGCATATCGCCCTTTGGAGACTTGTCCTCGGTCAAAACTTTCCTCGGGAGGCCATGGAAGAATTACTCCACAACTGTGTACATGCAAACATACATGGGAAATCTCATCAGCACTGATGGATGGTTGCCATGGGTAGGCAATAGCGCTCCCGACACCGTATTTTACTCGGAATTTCAAAATTTTGGACCTGGTGCTTCCACAAAGAATAGGGTTAAATGGAAGGGGTTGAGAACCATCAACCAAAAAGCAGCCAGCAAATTTACAGTGCATTCATTTCTCCAAGGAACTAAGTGGATCTCTGATGCTGGTGTTACCTACACATCAAGCCTCTGA

Protein Analysis

573

Amino Acids

62.59

Weight (kDa)

9.19

Isoelectric Point (pI)

27.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 67 - 214 1.7e-32 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 266 - 557 4.8e-137 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10710 AT5G04960
fragaria_vesca FvH4_7g04230 FvH4_7g04920 FvH4_7g04920
malus_domestica MD02G1266400.v1.1 MD07G1053300.v1.1
prunus_persica Prupe.2G058300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0331001
rosa_laevigata RLG00000029761
rosa_roxburghii Rroxscaffold_4G00319560
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113100 Rh1BG087100 Rh1CG110000 Rh1DG118800
rosa_wichuraiana Rw1G009220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1117
AccII CGCG 1 cut(s) 1218
AccIII TCCGGA 1 cut(s) 862
AciI CCGC 2 cut(s) 342, 1216
AclI AACGTT 1 cut(s) 1002
AclWI GGATC 5 cut(s) 453, 698, 762, 1343, 1688
AcoI YGGCCR 1 cut(s) 186
AcsI RAATTY 4 cut(s) 1368, 1556, 1564, 1643
AcuI CTGAAG 2 cut(s) 507, 752
AfaI GTAC 4 cut(s) 230, 719, 732, 1466
AfeI AGCGCT 1 cut(s) 1531
AfiI CCNNNNNNNGG 3 cut(s) 1117, 1310, 1520
AgsI TTSAA 5 cut(s) 301, 412, 551, 604, 1562
AhdI GACNNNNNGTC 3 cut(s) 394, 463, 475
AjnI CCWGG 5 cut(s) 231, 723, 1110, 1176, 1573
AjuI GAANNNNNNNTTGG 2 cut(s) 1193, 1225
AleI CACNNNNGTG 1 cut(s) 1460
AloI GAACNNNNNNTCC 2 cut(s) 135, 167
AluBI AGCT 4 cut(s) 407, 519, 763, 1334
AluI AGCT 4 cut(s) 407, 519, 763, 1334
Alw21I GWGCWC 2 cut(s) 521, 1275
Alw26I GTCTC 2 cut(s) 649, 1398
AlwI GGATC 5 cut(s) 453, 698, 762, 1343, 1688
Ama87I CYCGRG 2 cut(s) 1068, 1430
Aor13HI TCCGGA 1 cut(s) 862
Aor51HI AGCGCT 1 cut(s) 1531
AoxI GGCC 5 cut(s) 186, 1097, 1119, 1312, 1436
ApeKI GCWGC 6 cut(s) 131, 404, 742, 763, 1386, 1634
ApoI RAATTY 4 cut(s) 1368, 1556, 1564, 1643
AspLEI GCGC 1 cut(s) 1532
AspS9I GGNCC 3 cut(s) 257, 1097, 1571
AsuC2I CCSGG 3 cut(s) 49, 1069, 1070
AsuHPI GGTGA 1 cut(s) 974
AsuII TTCGAA 1 cut(s) 971
AvaI CYCGRG 2 cut(s) 1068, 1430
AvaII GGWCC 2 cut(s) 257, 1571
AxyI CCTNAGG 3 cut(s) 288, 680, 1296
BanII GRGCYC 1 cut(s) 521
BbsI GAAGAC 1 cut(s) 1141
Bbv12I GWGCWC 2 cut(s) 521, 1275
BbvI GCAGC 6 cut(s) 118, 416, 729, 750, 1398, 1646
BceAI ACGGC 1 cut(s) 1028
BcgI CGANNNNNNTGC 4 cut(s) 616, 650, 1151, 1185
BciT130I CCWGG 5 cut(s) 233, 725, 1112, 1178, 1575
BclI TGATCA 1 cut(s) 1138
BcnI CCSGG 3 cut(s) 49, 1069, 1070
BcoDI GTCTC 2 cut(s) 649, 1398
BfaI CTAG 1 cut(s) 624
BfmI CTRYAG 2 cut(s) 1092, 1153
BfoI RGCGCY 1 cut(s) 1533
BisI GCNGC 7 cut(s) 132, 342, 405, 743, 764, 1387, 1635
BlpI GCTNAGC 1 cut(s) 515
BlsI GCNGC 7 cut(s) 133, 343, 406, 744, 765, 1388, 1636
Bme1390I CCNGG 8 cut(s) 49, 233, 725, 1069, 1070, 1112, 1178, 1575
Bme18I GGWCC 2 cut(s) 257, 1571
BmeRI GACNNNNNGTC 3 cut(s) 394, 463, 475
BmeT110I CYCGRG 2 cut(s) 1068, 1430
BmgT120I GGNCC 3 cut(s) 257, 1097, 1571
BmiI GGNNCC 2 cut(s) 145, 1302
BmrFI CCNGG 8 cut(s) 49, 233, 725, 1069, 1070, 1112, 1178, 1575
BmsI GCATC 4 cut(s) 754, 835, 1159, 1678
BpiI GAAGAC 1 cut(s) 1141
Bpu1102I GCTNAGC 1 cut(s) 515
Bpu14I TTCGAA 1 cut(s) 971
BpuEI CTTGAG 3 cut(s) 488, 563, 1320
BpuMI CCSGG 3 cut(s) 49, 1069, 1070
BsaAI YACGTR 1 cut(s) 717
BsaBI GATNNNNATC 1 cut(s) 1353
BsaJI CCNNGG 8 cut(s) 384, 724, 1068, 1413, 1429, 1439, 1514, 1666
BsaWI WCCGGW 1 cut(s) 862
Bsc4I CCNNNNNNNGG 3 cut(s) 1117, 1310, 1520
Bse21I CCTNAGG 3 cut(s) 288, 680, 1296
Bse3DI GCAATG 2 cut(s) 820, 1062
Bse8I GATNNNNATC 1 cut(s) 1353
BseAI TCCGGA 1 cut(s) 862
BseBI CCWGG 5 cut(s) 233, 725, 1112, 1178, 1575
BseDI CCNNGG 8 cut(s) 384, 724, 1068, 1413, 1429, 1439, 1514, 1666
BseGI GGATG 3 cut(s) 850, 1015, 1511
BseJI GATNNNNATC 1 cut(s) 1353
BseLI CCNNNNNNNGG 3 cut(s) 1117, 1310, 1520
BseMI GCAATG 2 cut(s) 820, 1062
BseMII CTCAG 3 cut(s) 279, 506, 1275
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 6 cut(s) 118, 416, 729, 750, 1398, 1646
Bsh1236I CGCG 1 cut(s) 1218
BshFI GGCC 5 cut(s) 188, 1099, 1121, 1314, 1438
BsiHKAI GWGCWC 2 cut(s) 521, 1275
BsiHKCI CYCGRG 2 cut(s) 1068, 1430
BsiSI CCGG 3 cut(s) 49, 863, 1069
BslFI GGGAC 1 cut(s) 1085
BslI CCNNNNNNNGG 3 cut(s) 1117, 1310, 1520
BsmAI GTCTC 2 cut(s) 649, 1398
BsmFI GGGAC 1 cut(s) 1085
BsmI GAATGC 4 cut(s) 979, 1172, 1226, 1654
BsnI GGCC 5 cut(s) 188, 1099, 1121, 1314, 1438
BsoBI CYCGRG 2 cut(s) 1068, 1430
Bsp119I TTCGAA 1 cut(s) 971
Bsp1286I GDGCHC 2 cut(s) 521, 1275
Bsp13I TCCGGA 1 cut(s) 862
Bsp1407I TGTACA 1 cut(s) 1464
Bsp143I GATC 8 cut(s) 321, 445, 499, 703, 754, 1138, 1348, 1680
Bsp1720I GCTNAGC 1 cut(s) 515
Bsp19I CCATGG 2 cut(s) 1439, 1514
BspACI CCGC 2 cut(s) 342, 1216
BspANI GGCC 5 cut(s) 188, 1099, 1121, 1314, 1438
BspCNI CTCAG 3 cut(s) 280, 507, 1274
BspEI TCCGGA 1 cut(s) 862
BspFNI CGCG 1 cut(s) 1218
BspLI GGNNCC 2 cut(s) 145, 1302
BspMAI CTGCAG 1 cut(s) 1096
BspPI GGATC 5 cut(s) 453, 698, 762, 1343, 1688
BspQI GCTCTTC 1 cut(s) 526
BspT104I TTCGAA 1 cut(s) 971
BsrDI GCAATG 2 cut(s) 820, 1062
BsrGI TGTACA 1 cut(s) 1464
BssECI CCNNGG 8 cut(s) 384, 724, 1068, 1413, 1429, 1439, 1514, 1666
BssMI GATC 8 cut(s) 321, 445, 499, 703, 754, 1138, 1348, 1680
BssT1I CCWWGG 4 cut(s) 384, 1439, 1514, 1666
Bst2UI CCWGG 5 cut(s) 233, 725, 1112, 1178, 1575
Bst4CI ACNGT 8 cut(s) 151, 396, 886, 1039, 1240, 1462, 1543, 1651
Bst6I CTCTTC 2 cut(s) 526, 872
BstAPI GCANNNNNTGC 1 cut(s) 854
BstAUI TGTACA 1 cut(s) 1464
BstBAI YACGTR 1 cut(s) 717
BstBI TTCGAA 1 cut(s) 971
BstC8I GCNNGC 5 cut(s) 761, 811, 1101, 1316, 1639
BstDEI CTNAG 8 cut(s) 288, 474, 515, 670, 680, 1261, 1296, 1674
BstDSI CCRYGG 2 cut(s) 1439, 1514
BstF5I GGATG 3 cut(s) 850, 1015, 1511
BstFNI CGCG 1 cut(s) 1218
BstH2I RGCGCY 1 cut(s) 1533
BstHHI GCGC 1 cut(s) 1532
BstKTI GATC 8 cut(s) 324, 448, 502, 706, 757, 1141, 1351, 1683
BstMAI GTCTC 2 cut(s) 649, 1398
BstMBI GATC 8 cut(s) 321, 445, 499, 703, 754, 1138, 1348, 1680
BstMWI GCNNNNNNNGC 6 cut(s) 525, 854, 1040, 1100, 1105, 1315
BstNI CCWGG 5 cut(s) 233, 725, 1112, 1178, 1575
BstNSI RCATGY 1 cut(s) 1471
BstSCI CCNGG 8 cut(s) 47, 231, 723, 1067, 1068, 1110, 1176, 1573
BstSFI CTRYAG 2 cut(s) 1092, 1153
BstSNI TACGTA 1 cut(s) 717
BstUI CGCG 1 cut(s) 1218
BstV1I GCAGC 6 cut(s) 118, 416, 729, 750, 1398, 1646
BstV2I GAAGAC 1 cut(s) 1141
BstX2I RGATCY 1 cut(s) 1680
BstYI RGATCY 1 cut(s) 1680
Bsu36I CCTNAGG 3 cut(s) 288, 680, 1296
BsuRI GGCC 5 cut(s) 188, 1099, 1121, 1314, 1438
BtgI CCRYGG 2 cut(s) 1439, 1514
BtsCI GGATG 3 cut(s) 850, 1015, 1511
BtsI GCAGTG 3 cut(s) 1029, 1089, 1122
BtsIMutI CAGTG 6 cut(s) 1029, 1089, 1122, 1245, 1497, 1656
Cac8I GCNNGC 5 cut(s) 761, 811, 1101, 1316, 1639
CfoI GCGC 1 cut(s) 1532
Cfr13I GGNCC 3 cut(s) 257, 1097, 1571
Cfr9I CCCGGG 1 cut(s) 1068
CseI GACGC 1 cut(s) 65
CsiI ACCWGGT 1 cut(s) 1573
Csp6I GTAC 4 cut(s) 229, 718, 731, 1465
CspCI CAANNNNNGTGG 2 cut(s) 297, 332
CviAII CATG 5 cut(s) 1306, 1440, 1468, 1480, 1515
CviQI GTAC 4 cut(s) 229, 718, 731, 1465
DdeI CTNAG 8 cut(s) 288, 474, 515, 670, 680, 1261, 1296, 1674
DpnI GATC 8 cut(s) 323, 447, 501, 705, 756, 1140, 1350, 1682
DpnII GATC 8 cut(s) 321, 445, 499, 703, 754, 1138, 1348, 1680
DriI GACNNNNNGTC 3 cut(s) 394, 463, 475
EaeI YGGCCR 1 cut(s) 186
Eam1104I CTCTTC 2 cut(s) 526, 872
Eam1105I GACNNNNNGTC 3 cut(s) 394, 463, 475
EarI CTCTTC 2 cut(s) 526, 872
Ecl136II GAGCTC 1 cut(s) 519
Eco105I TACGTA 1 cut(s) 717
Eco130I CCWWGG 4 cut(s) 384, 1439, 1514, 1666
Eco24I GRGCYC 1 cut(s) 521
Eco47I GGWCC 2 cut(s) 257, 1571
Eco47III AGCGCT 1 cut(s) 1531
Eco53kI GAGCTC 1 cut(s) 519
Eco57I CTGAAG 2 cut(s) 507, 752
Eco81I CCTNAGG 3 cut(s) 288, 680, 1296
Eco88I CYCGRG 2 cut(s) 1068, 1430
EcoICRI GAGCTC 1 cut(s) 519
EcoRII CCWGG 5 cut(s) 231, 723, 1110, 1176, 1573
EcoT14I CCWWGG 4 cut(s) 384, 1439, 1514, 1666
EcoT22I ATGCAT 1 cut(s) 1174
EcoT38I GRGCYC 1 cut(s) 521
ErhI CCWWGG 4 cut(s) 384, 1439, 1514, 1666
FaeI CATG 5 cut(s) 1309, 1443, 1471, 1483, 1518
FaqI GGGAC 1 cut(s) 1085
FatI CATG 5 cut(s) 1305, 1439, 1467, 1479, 1514
FbaI TGATCA 1 cut(s) 1138
Fnu4HI GCNGC 7 cut(s) 132, 342, 405, 743, 764, 1387, 1635
FokI GGATG 3 cut(s) 857, 1022, 1518
FriOI GRGCYC 1 cut(s) 521
Fsp4HI GCNGC 7 cut(s) 132, 342, 405, 743, 764, 1387, 1635
FspBI CTAG 1 cut(s) 624
GlaI GCGC 1 cut(s) 1531
GluI GCNGC 7 cut(s) 132, 342, 405, 743, 764, 1387, 1635
HaeII RGCGCY 1 cut(s) 1533
HaeIII GGCC 5 cut(s) 188, 1099, 1121, 1314, 1438
HapII CCGG 3 cut(s) 49, 863, 1069
HgaI GACGC 1 cut(s) 65
HhaI GCGC 1 cut(s) 1532
Hin1II CATG 5 cut(s) 1309, 1443, 1471, 1483, 1518
Hin6I GCGC 1 cut(s) 1530
HinP1I GCGC 1 cut(s) 1530
HincII GTYRAC 3 cut(s) 268, 621, 1131
HindII GTYRAC 3 cut(s) 268, 621, 1131
HinfI GANTC 6 cut(s) 35, 236, 433, 458, 1059, 1375
HpaI GTTAAC 2 cut(s) 268, 621
HpaII CCGG 3 cut(s) 49, 863, 1069
HphI GGTGA 1 cut(s) 974
Hpy166II GTNNAC 5 cut(s) 268, 621, 676, 1131, 1465
Hpy188I TCNGA 9 cut(s) 40, 469, 693, 750, 771, 778, 1555, 1687, 1718
Hpy188III TCNNGA 8 cut(s) 422, 505, 842, 863, 898, 1255, 1432, 1535
Hpy8I GTNNAC 5 cut(s) 268, 621, 676, 1131, 1465
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 5 cut(s) 545, 745, 784, 895, 1600
HpyCH4III ACNGT 8 cut(s) 151, 396, 886, 1039, 1240, 1462, 1543, 1651
HpyCH4IV ACGT 3 cut(s) 716, 894, 1002
HpyF10VI GCNNNNNNNGC 6 cut(s) 525, 854, 1040, 1100, 1105, 1315
HpyF3I CTNAG 8 cut(s) 288, 474, 515, 670, 680, 1261, 1296, 1674
HpySE526I ACGT 3 cut(s) 716, 894, 1002
Hsp92II CATG 5 cut(s) 1309, 1443, 1471, 1483, 1518
HspAI GCGC 1 cut(s) 1530
Kpn2I TCCGGA 1 cut(s) 862
Ksp22I TGATCA 1 cut(s) 1138
KspAI GTTAAC 2 cut(s) 268, 621
Kzo9I GATC 8 cut(s) 321, 445, 499, 703, 754, 1138, 1348, 1680
LguI GCTCTTC 1 cut(s) 526
LmnI GCTCC 2 cut(s) 1278, 1537
Lsp1109I GCAGC 6 cut(s) 118, 416, 729, 750, 1398, 1646
LweI GCATC 4 cut(s) 754, 835, 1159, 1678
MabI ACCWGGT 1 cut(s) 1573
MaeI CTAG 1 cut(s) 624
MaeII ACGT 3 cut(s) 716, 894, 1002
MaeIII GTNAC 4 cut(s) 220, 242, 985, 1696
MalI GATC 8 cut(s) 323, 447, 501, 705, 756, 1140, 1350, 1682
MboI GATC 8 cut(s) 321, 445, 499, 703, 754, 1138, 1348, 1680
MboII GAAGA 6 cut(s) 513, 616, 799, 889, 1141, 1456
MfeI CAATTG 1 cut(s) 1381
MflI RGATCY 1 cut(s) 1680
MhlI GDGCHC 2 cut(s) 521, 1275
MlyI GAGTC 2 cut(s) 442, 452
MmeI TCCRAC 3 cut(s) 63, 121, 297
MnlI CCTC 9 cut(s) 270, 283, 644, 777, 918, 1304, 1423, 1428, 1439
Mph1103I ATGCAT 1 cut(s) 1174
MroI TCCGGA 1 cut(s) 862
MseI TTAA 6 cut(s) 267, 282, 372, 620, 666, 1599
MslI CAYNNNNRTG 2 cut(s) 803, 1460
MspI CCGG 3 cut(s) 49, 863, 1069
MspR9I CCNGG 8 cut(s) 49, 233, 725, 1069, 1070, 1112, 1178, 1575
MunI CAATTG 1 cut(s) 1381
Mva1269I GAATGC 4 cut(s) 979, 1172, 1226, 1654
MvaI CCWGG 5 cut(s) 233, 725, 1112, 1178, 1575
MvnI CGCG 1 cut(s) 1218
MwoI GCNNNNNNNGC 6 cut(s) 525, 854, 1040, 1100, 1105, 1315
NciI CCSGG 3 cut(s) 49, 1069, 1070
NcoI CCATGG 2 cut(s) 1439, 1514
NdeII GATC 8 cut(s) 321, 445, 499, 703, 754, 1138, 1348, 1680
NlaIII CATG 5 cut(s) 1309, 1443, 1471, 1483, 1518
NlaIV GGNNCC 2 cut(s) 145, 1302
NmeAIII GCCGAG 1 cut(s) 164
NmuCI GTSAC 1 cut(s) 220
NsiI ATGCAT 1 cut(s) 1174
NspI RCATGY 1 cut(s) 1471
NspV TTCGAA 1 cut(s) 971
OliI CACNNNNGTG 1 cut(s) 1460
PciSI GCTCTTC 1 cut(s) 526
PctI GAATGC 4 cut(s) 979, 1172, 1226, 1654
PfeI GAWTC 4 cut(s) 35, 236, 1059, 1375
PflFI GACNNNGTC 1 cut(s) 800
PflMI CCANNNNNTGG 1 cut(s) 1117
PfoI TCCNGGA 1 cut(s) 1176
PkrI GCNGC 7 cut(s) 133, 343, 406, 744, 765, 1388, 1636
PleI GAGTC 2 cut(s) 441, 452
PpsI GAGTC 2 cut(s) 441, 452
Ppu21I YACGTR 1 cut(s) 717
Psp124BI GAGCTC 1 cut(s) 521
Psp1406I AACGTT 1 cut(s) 1002
Psp6I CCWGG 5 cut(s) 231, 723, 1110, 1176, 1573
PspGI CCWGG 5 cut(s) 231, 723, 1110, 1176, 1573
PspN4I GGNNCC 2 cut(s) 145, 1302
PspPI GGNCC 3 cut(s) 257, 1097, 1571
PstI CTGCAG 1 cut(s) 1096
PsuI RGATCY 1 cut(s) 1680
PsyI GACNNNGTC 1 cut(s) 800
RsaI GTAC 4 cut(s) 230, 719, 732, 1466
RsaNI GTAC 4 cut(s) 229, 718, 731, 1465
RseI CAYNNNNRTG 2 cut(s) 803, 1460
SacI GAGCTC 1 cut(s) 521
SapI GCTCTTC 1 cut(s) 526
SaqAI TTAA 6 cut(s) 267, 282, 372, 620, 666, 1599
SatI GCNGC 7 cut(s) 132, 342, 405, 743, 764, 1387, 1635
Sau3AI GATC 8 cut(s) 321, 445, 499, 703, 754, 1138, 1348, 1680
Sau96I GGNCC 3 cut(s) 257, 1097, 1571
SchI GAGTC 2 cut(s) 442, 452
ScrFI CCNGG 8 cut(s) 49, 233, 725, 1069, 1070, 1112, 1178, 1575
SduI GDGCHC 2 cut(s) 521, 1275
SexAI ACCWGGT 1 cut(s) 1573
SfaNI GCATC 4 cut(s) 754, 835, 1159, 1678
SfcI CTRYAG 2 cut(s) 1092, 1153
SfuI TTCGAA 1 cut(s) 971
SinI GGWCC 2 cut(s) 257, 1571
SmaI CCCGGG 1 cut(s) 1070
SmiMI CAYNNNNRTG 2 cut(s) 803, 1460
SmlI CTYRAG 3 cut(s) 503, 578, 1335
SmoI CTYRAG 3 cut(s) 503, 578, 1335
SnaBI TACGTA 1 cut(s) 717
SsiI CCGC 2 cut(s) 342, 1216
SspMI CTAG 1 cut(s) 624
SstI GAGCTC 1 cut(s) 521
StyD4I CCNGG 8 cut(s) 47, 231, 723, 1067, 1068, 1110, 1176, 1573
StyI CCWWGG 4 cut(s) 384, 1439, 1514, 1666
TaaI ACNGT 8 cut(s) 151, 396, 886, 1039, 1240, 1462, 1543, 1651
TaiI ACGT 3 cut(s) 719, 897, 1005
TaqI TCGA 5 cut(s) 54, 73, 335, 971, 1373
TaqII GACCGA 1 cut(s) 1405
TatI WGTACW 1 cut(s) 1464
TauI GCSGC 1 cut(s) 344
TfiI GAWTC 4 cut(s) 35, 236, 1059, 1375
Tru1I TTAA 6 cut(s) 267, 282, 372, 620, 666, 1599
Tru9I TTAA 6 cut(s) 267, 282, 372, 620, 666, 1599
TscAI CASTG 6 cut(s) 1036, 1096, 1122, 1245, 1504, 1656
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 6 cut(s) 131, 404, 742, 763, 1386, 1634
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 6 cut(s) 48, 143, 342, 1051, 1237, 1647
TspMI CCCGGG 1 cut(s) 1068
TspRI CASTG 6 cut(s) 1036, 1096, 1122, 1245, 1504, 1656
Tth111I GACNNNGTC 1 cut(s) 800
Van91I CCANNNNNTGG 1 cut(s) 1117
VpaK11BI GGWCC 2 cut(s) 257, 1571
XapI RAATTY 4 cut(s) 1368, 1556, 1564, 1643
XceI RCATGY 1 cut(s) 1471
XmaI CCCGGG 1 cut(s) 1068
XspI CTAG 1 cut(s) 624
Zsp2I ATGCAT 1 cut(s) 1174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.