Rw1G009220

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0013785
Physical Location & Seq
Reverse (-)
20306613 .. 20308761
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G009220.1

Sequence Viewer

Length: 1617 bp
ATGTCTTCCTTCAAAGCTTACGGCAAAGTCAGTGAATCCGACCAAGCCAAGCTTGATGCTCGTCGCAAGACTTGCAGGAGAGTCGCCATCATATCCATTTCCTCCATAGTTCTTGTCTGCGTCGTAGTTGCCGCCGTGGTTGGAACCGCTACTCATAACTCCAAAAAAGGTGACGACAATGGCAAAGGTACGCTTTCCGCTTCCGTCAAAGCCGTTTGTGACGTGACATTGTACCAGGACTCGTGTTATTCTAGTCTTGGTCCTATTGTAAACTCCAGCAATTTTAAGCCTGAGGATTTGTTCAAGTTGTCAATTCAAGTGGCAATTACTGAACTGTCCAAAGCTGCTACTCAAATTTCCGATCAAGGGCTTTTCAAAGGTACTGTCACGGACAAATTGTCCGTGGCAGCTTTGAAAAACTGTGGCGAGCTTCTGAGTCTTGCCTTGGATCATCTCAACGAGTCGTTGTCCGGGGATGGTTCCTTGGTCGAAGTGGTTGAAGATCTTAAAACATGGTTGAGCTCTGCAGGAACTTACCAGCAGTCATGTGTAGATGGGTTTGAGGATGCAAAAGGAGATCTAAAAGCTACCATTGAAAAGTATCTCAAGACTTCTACTGAACTAACTAGTAACAGCCTTGCCATTGTCTCTTGGATTTCGAAAGTAGCCAACTCTGTGAAGCTAAGGCGGTTGCTGAGTCACGATGGTGCCGCAGCTAGCCATGGCATGCCGGAGTGGCTGCACCAGACAGACCGCAGGCTAATTGAAAGTTCAGATTTGCGGAATAAGGCCAACATTGTTGTGGCCAAAGATGGGAGTGGCAAGTACAAGACAGTAGGTGCTGCACTGAAGGCTGTGCCGGACAAAAGCAAGAAGAGAACAGTCATCTACGTGAAGAAGGGTGTTTATTTCGAGAATGTTCGAGTGGAGAAGTCCAAGTGGAATGTTGTGGTGGGATTCGTCGCCAGAGACATTGGAATTCGTAACACAGCCGGAGCGGCCAAGCACCAGGCGGTGGCACTAATGTCGAGTGCTGACCAGACGGTGTTCTACAGATGCCACATTGATGCTTTCCAGGACTCGCTCTACGTTCATGCCAATCGCCAATTCTACCGGGAATGCAACATTTACGGAACAGTTGATTTCATTTTCGGAAACTCAGCAGTAGTCCTCCAAAACTGTAAGGTGTTCCCCAAGGTACCAATGGTTGGCCAGCAGAACACAATCACGGCACAAGGCAAAATCGACCCCAACCAAAACACGGGAATCGCATTTCAGAACTGCACCATTTCCCCTTTTGGAGACTTGTCTTCGGTTAAGACTTACCTCGGGAGACCTTGGAAGAATTACTCCACAACAGTGTACATGCAAACATTCATGGGAAGCTTGATTCACCCAGATGGATGGTTGCCATGGGTAGGCACAACGGCACCTGACTCCATTTTTTACTCCGAATTTCAGAATTTCGGACCTGGTTCTGTGACAAAGAATAGAGTTAAGTGGAAGGGCTTGAAAACAATAACCAGCAAAGTGGCCGGCAAATTTACCGTGCACTCATTTCTTGAAGGAACCAATTGGATCTCCGATGCTAGTGTTCCCTATAAATCCAACCTCTGA

Protein Analysis

538

Amino Acids

58.68

Weight (kDa)

9.25

Isoelectric Point (pI)

29.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 68 - 215 6.5e-32 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 265 - 318 8.1e-17 Pectinesterase
Pectinesterase PF01095 319 - 523 4.4e-96 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013785)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10710 AT5G04960
fragaria_vesca FvH4_7g04230 FvH4_7g04920 FvH4_7g04920
malus_domestica MD02G1266400.v1.1 MD07G1053300.v1.1
prunus_persica Prupe.2G058300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0331001
rosa_laevigata RLG00000029761
rosa_roxburghii Rroxscaffold_4G00319560
rosa_rugosa Rorug01G0091500
rosa_samantha Rh1AG113100 Rh1BG087100 Rh1CG110000 Rh1DG118800
rosa_wichuraiana Rw1G009220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1198
AccB1I GGYRCC 3 cut(s) 707, 1198, 1429
AccB7I CCANNNNNTGG 2 cut(s) 1015, 1208
AccBSI CCGCTC 1 cut(s) 998
AciI CCGC 9 cut(s) 132, 147, 198, 688, 711, 754, 781, 998, 1013
AclWI GGATC 2 cut(s) 456, 1586
AcoI YGGCCR 4 cut(s) 804, 999, 1210, 1533
AcsI RAATTY 5 cut(s) 354, 978, 1454, 1462, 1541
AcuI CTGAAG 1 cut(s) 869
AfaI GTAC 6 cut(s) 190, 233, 382, 827, 1200, 1364
AfiI CCNNNNNNNGG 6 cut(s) 366, 813, 1015, 1208, 1261, 1418
AhdI GACNNNNNGTC 1 cut(s) 397
AhlI ACTAGT 1 cut(s) 626
AjiI CACGTC 1 cut(s) 223
AjnI CCWGG 4 cut(s) 234, 1008, 1074, 1471
AjuI GAANNNNNNNTTGG 2 cut(s) 1091, 1123
AleI CACNNNNGTG 2 cut(s) 705, 1358
Alw21I GWGCWC 2 cut(s) 524, 1554
Alw26I GTCTC 4 cut(s) 652, 963, 1296, 1327
Alw44I GTGCAC 1 cut(s) 1550
AlwI GGATC 2 cut(s) 456, 1586
Ama87I CYCGRG 1 cut(s) 1328
AoxI GGCC 5 cut(s) 789, 804, 999, 1210, 1533
ApaLI GTGCAC 1 cut(s) 1550
ApeKI GCWGC 5 cut(s) 344, 407, 713, 739, 842
ApoI RAATTY 5 cut(s) 354, 978, 1454, 1462, 1541
ArsI GACNNNNNNTTYG 2 cut(s) 369, 401
Asp718I GGTACC 1 cut(s) 1198
AspS9I GGNCC 2 cut(s) 260, 1469
AsuC2I CCSGG 2 cut(s) 472, 1115
AsuHPI GGTGA 2 cut(s) 182, 1385
AsuII TTCGAA 1 cut(s) 659
AsuNHI GCTAGC 1 cut(s) 716
AvaI CYCGRG 1 cut(s) 1328
AvaII GGWCC 2 cut(s) 260, 1469
AxyI CCTNAGG 1 cut(s) 291
BaeGI GKGCMC 1 cut(s) 1554
BalI TGGCCA 2 cut(s) 806, 1212
BanI GGYRCC 3 cut(s) 707, 1198, 1429
BanII GRGCYC 1 cut(s) 524
BauI CACGAG 1 cut(s) 241
BbsI GAAGAC 1 cut(s) 1302
Bbv12I GWGCWC 2 cut(s) 524, 1554
BbvI GCAGC 5 cut(s) 331, 419, 725, 726, 829
BccI CCATC 7 cut(s) 95, 470, 548, 698, 806, 1394, 1398
BceAI ACGGC 5 cut(s) 37, 119, 197, 1245, 1443
BcgI CGANNNNNNTGC 4 cut(s) 64, 98, 1008, 1042
BciT130I CCWGG 4 cut(s) 236, 1010, 1076, 1473
BcnI CCSGG 2 cut(s) 472, 1115
BcoDI GTCTC 4 cut(s) 652, 963, 1296, 1327
BcuI ACTAGT 1 cut(s) 626
BfaI CTAG 4 cut(s) 252, 627, 717, 1590
BfmI CTRYAG 2 cut(s) 525, 1051
BglI GCCNNNNNGGC 2 cut(s) 736, 998
BglII AGATCT 2 cut(s) 502, 577
BisI GCNGC 8 cut(s) 132, 345, 408, 711, 714, 740, 843, 999
BlsI GCNGC 8 cut(s) 133, 346, 409, 712, 715, 741, 844, 1000
Bme1390I CCNGG 6 cut(s) 236, 472, 1010, 1076, 1115, 1473
Bme18I GGWCC 2 cut(s) 260, 1469
BmeRI GACNNNNNGTC 1 cut(s) 397
BmeT110I CYCGRG 1 cut(s) 1328
BmgBI CACGTC 1 cut(s) 223
BmgT120I GGNCC 2 cut(s) 260, 1469
BmiI GGNNCC 6 cut(s) 145, 481, 709, 1200, 1431, 1570
BmrFI CCNGG 6 cut(s) 236, 472, 1010, 1076, 1115, 1473
BmsI GCATC 5 cut(s) 46, 556, 1046, 1057, 1576
BmtI GCTAGC 1 cut(s) 720
BpiI GAAGAC 1 cut(s) 1302
BpmI CTGGAG 1 cut(s) 259
Bpu10I CCTNAGC 1 cut(s) 683
Bpu14I TTCGAA 1 cut(s) 659
BpuEI CTTGAG 1 cut(s) 590
BpuMI CCSGG 2 cut(s) 472, 1115
BsaAI YACGTR 1 cut(s) 892
BsaI GGTCTC 1 cut(s) 1327
Bsc4I CCNNNNNNNGG 6 cut(s) 366, 813, 1015, 1208, 1261, 1418
Bse118I RCCGGY 1 cut(s) 1535
Bse21I CCTNAGG 1 cut(s) 291
BseBI CCWGG 4 cut(s) 236, 1010, 1076, 1473
BseGI GGATG 3 cut(s) 481, 571, 1409
BseLI CCNNNNNNNGG 6 cut(s) 366, 813, 1015, 1208, 1261, 1418
BseMII CTCAG 4 cut(s) 282, 425, 686, 1173
BseSI GKGCMC 1 cut(s) 1554
BseXI GCAGC 5 cut(s) 331, 419, 725, 726, 829
BsgI GTGCAG 3 cut(s) 725, 828, 1267
BshFI GGCC 5 cut(s) 791, 806, 1001, 1212, 1535
BshNI GGYRCC 3 cut(s) 707, 1198, 1429
BsiHKAI GWGCWC 2 cut(s) 524, 1554
BsiHKCI CYCGRG 1 cut(s) 1328
BsiSI CCGG 6 cut(s) 471, 731, 860, 993, 1114, 1536
BslI CCNNNNNNNGG 6 cut(s) 366, 813, 1015, 1208, 1261, 1418
BsmAI GTCTC 4 cut(s) 652, 963, 1296, 1327
BsmI GAATGC 1 cut(s) 1124
BsnI GGCC 5 cut(s) 791, 806, 1001, 1212, 1535
Bso31I GGTCTC 1 cut(s) 1327
BsoBI CYCGRG 1 cut(s) 1328
Bsp119I TTCGAA 1 cut(s) 659
Bsp1286I GDGCHC 2 cut(s) 524, 1554
Bsp1407I TGTACA 1 cut(s) 1362
Bsp143I GATC 5 cut(s) 361, 448, 502, 577, 1578
Bsp19I CCATGG 2 cut(s) 721, 1412
BspACI CCGC 9 cut(s) 132, 147, 198, 688, 711, 754, 781, 998, 1013
BspANI GGCC 5 cut(s) 791, 806, 1001, 1212, 1535
BspCNI CTCAG 4 cut(s) 283, 426, 687, 1172
BspLI GGNNCC 6 cut(s) 145, 481, 709, 1200, 1431, 1570
BspMAI CTGCAG 1 cut(s) 529
BspOI GCTAGC 1 cut(s) 720
BspPI GGATC 2 cut(s) 456, 1586
BspT104I TTCGAA 1 cut(s) 659
BspT107I GGYRCC 3 cut(s) 707, 1198, 1429
BspTNI GGTCTC 1 cut(s) 1327
BsrBI CCGCTC 1 cut(s) 998
BsrFI RCCGGY 1 cut(s) 1535
BsrGI TGTACA 1 cut(s) 1362
BssAI RCCGGY 1 cut(s) 1535
BssMI GATC 5 cut(s) 361, 448, 502, 577, 1578
BssSI CACGAG 1 cut(s) 241
BssT1I CCWWGG 6 cut(s) 444, 483, 721, 1194, 1337, 1412
Bst2BI CACGAG 1 cut(s) 241
Bst2UI CCWGG 4 cut(s) 236, 1010, 1076, 1473
Bst6I CTCTTC 1 cut(s) 869
BstAPI GCANNNNNTGC 1 cut(s) 72
BstAUI TGTACA 1 cut(s) 1362
BstBAI YACGTR 1 cut(s) 892
BstBI TTCGAA 1 cut(s) 659
BstC8I GCNNGC 6 cut(s) 428, 718, 728, 758, 1214, 1537
BstDEI CTNAG 5 cut(s) 291, 434, 683, 695, 1159
BstDSI CCRYGG 4 cut(s) 135, 402, 721, 1412
BstF5I GGATG 3 cut(s) 481, 571, 1409
BstKTI GATC 5 cut(s) 364, 451, 505, 580, 1581
BstMAI GTCTC 4 cut(s) 652, 963, 1296, 1327
BstMBI GATC 5 cut(s) 361, 448, 502, 577, 1578
BstMWI GCNNNNNNNGC 4 cut(s) 72, 736, 851, 998
BstNI CCWGG 4 cut(s) 236, 1010, 1076, 1473
BstNSI RCATGY 2 cut(s) 730, 1369
BstSCI CCNGG 6 cut(s) 234, 470, 1008, 1074, 1113, 1471
BstSFI CTRYAG 2 cut(s) 525, 1051
BstSLI GKGCMC 1 cut(s) 1554
BstV1I GCAGC 5 cut(s) 331, 419, 725, 726, 829
BstV2I GAAGAC 1 cut(s) 1302
BstX2I RGATCY 3 cut(s) 502, 577, 1578
BstXI CCANNNNNNTGG 2 cut(s) 1404, 1531
BstYI RGATCY 3 cut(s) 502, 577, 1578
Bsu36I CCTNAGG 1 cut(s) 291
BsuRI GGCC 5 cut(s) 791, 806, 1001, 1212, 1535
BtgI CCRYGG 4 cut(s) 135, 402, 721, 1412
BtrI CACGTC 1 cut(s) 223
BtsCI GGATG 3 cut(s) 481, 571, 1409
BtsIMutI CAGTG 3 cut(s) 37, 845, 1365
Cac8I GCNNGC 6 cut(s) 428, 718, 728, 758, 1214, 1537
Cfr10I RCCGGY 1 cut(s) 1535
Cfr13I GGNCC 2 cut(s) 260, 1469
CseI GACGC 1 cut(s) 109
CsiI ACCWGGT 1 cut(s) 1471
Csp6I GTAC 6 cut(s) 189, 232, 381, 826, 1199, 1363
CspCI CAANNNNNGTGG 2 cut(s) 300, 335
CviAII CATG 8 cut(s) 513, 546, 722, 727, 1094, 1366, 1378, 1413
CviQI GTAC 6 cut(s) 189, 232, 381, 826, 1199, 1363
DdeI CTNAG 5 cut(s) 291, 434, 683, 695, 1159
DpnI GATC 5 cut(s) 363, 450, 504, 579, 1580
DpnII GATC 5 cut(s) 361, 448, 502, 577, 1578
DriI GACNNNNNGTC 1 cut(s) 397
EaeI YGGCCR 4 cut(s) 804, 999, 1210, 1533
Eam1104I CTCTTC 1 cut(s) 869
Eam1105I GACNNNNNGTC 1 cut(s) 397
EarI CTCTTC 1 cut(s) 869
Ecl136II GAGCTC 1 cut(s) 522
Eco130I CCWWGG 6 cut(s) 444, 483, 721, 1194, 1337, 1412
Eco24I GRGCYC 1 cut(s) 524
Eco31I GGTCTC 1 cut(s) 1327
Eco47I GGWCC 2 cut(s) 260, 1469
Eco53kI GAGCTC 1 cut(s) 522
Eco57I CTGAAG 1 cut(s) 869
Eco81I CCTNAGG 1 cut(s) 291
Eco88I CYCGRG 1 cut(s) 1328
EcoICRI GAGCTC 1 cut(s) 522
EcoRI GAATTC 1 cut(s) 978
EcoRII CCWGG 4 cut(s) 234, 1008, 1074, 1471
EcoT14I CCWWGG 6 cut(s) 444, 483, 721, 1194, 1337, 1412
EcoT38I GRGCYC 1 cut(s) 524
ErhI CCWWGG 6 cut(s) 444, 483, 721, 1194, 1337, 1412
FaeI CATG 8 cut(s) 516, 549, 725, 730, 1097, 1369, 1381, 1416
FalI AAGNNNNNCTT 4 cut(s) 36, 68, 177, 209
FatI CATG 8 cut(s) 512, 545, 721, 726, 1093, 1365, 1377, 1412
Fnu4HI GCNGC 8 cut(s) 132, 345, 408, 711, 714, 740, 843, 999
FokI GGATG 3 cut(s) 488, 578, 1416
FriOI GRGCYC 1 cut(s) 524
Fsp4HI GCNGC 8 cut(s) 132, 345, 408, 711, 714, 740, 843, 999
FspBI CTAG 4 cut(s) 252, 627, 717, 1590
GluI GCNGC 8 cut(s) 132, 345, 408, 711, 714, 740, 843, 999
GsuI CTGGAG 1 cut(s) 259
HaeIII GGCC 5 cut(s) 791, 806, 1001, 1212, 1535
HapII CCGG 6 cut(s) 471, 731, 860, 993, 1114, 1536
HgaI GACGC 1 cut(s) 109
Hin1II CATG 8 cut(s) 516, 549, 725, 730, 1097, 1369, 1381, 1416
HindIII AAGCTT 3 cut(s) 15, 50, 1384
HpaII CCGG 6 cut(s) 471, 731, 860, 993, 1114, 1536
HphI GGTGA 2 cut(s) 182, 1385
Hpy166II GTNNAC 3 cut(s) 271, 1363, 1552
Hpy188III TCNNGA 5 cut(s) 607, 701, 913, 1330, 1562
Hpy8I GTNNAC 3 cut(s) 271, 1363, 1552
Hpy99I CGWCG 3 cut(s) 66, 125, 965
HpyAV CCTTC 5 cut(s) 19, 844, 892, 1498, 1559
HpyCH4IV ACGT 3 cut(s) 222, 891, 1089
HpyCH4V TGCA 9 cut(s) 75, 527, 569, 742, 845, 1122, 1284, 1369, 1552
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 736, 851, 998
HpyF3I CTNAG 5 cut(s) 291, 434, 683, 695, 1159
HpySE526I ACGT 3 cut(s) 222, 891, 1089
Hsp92II CATG 8 cut(s) 516, 549, 725, 730, 1097, 1369, 1381, 1416
KpnI GGTACC 1 cut(s) 1202
KroI GCCGGC 1 cut(s) 1535
KroNI GCCGGC 1 cut(s) 1537
Kzo9I GATC 5 cut(s) 361, 448, 502, 577, 1578
LmnI GCTCC 1 cut(s) 995
Lsp1109I GCAGC 5 cut(s) 331, 419, 725, 726, 829
LweI GCATC 5 cut(s) 46, 556, 1046, 1057, 1576
MabI ACCWGGT 1 cut(s) 1471
MaeI CTAG 4 cut(s) 252, 627, 717, 1590
MaeII ACGT 3 cut(s) 222, 891, 1089
MaeIII GTNAC 8 cut(s) 170, 218, 223, 385, 629, 698, 983, 1480
MalI GATC 5 cut(s) 363, 450, 504, 579, 1580
MbiI CCGCTC 1 cut(s) 998
MboI GATC 5 cut(s) 361, 448, 502, 577, 1578
MboII GAAGA 5 cut(s) 512, 886, 907, 1302, 1354
MfeI CAATTG 1 cut(s) 1573
MflI RGATCY 3 cut(s) 502, 577, 1578
MhlI GDGCHC 2 cut(s) 524, 1554
MlsI TGGCCA 2 cut(s) 806, 1212
MluNI TGGCCA 2 cut(s) 806, 1212
MlyI GAGTC 7 cut(s) 90, 233, 445, 470, 706, 1073, 1430
MmeI TCCRAC 2 cut(s) 63, 121
MnlI CCTC 5 cut(s) 112, 286, 556, 1181, 1337
Mox20I TGGCCA 2 cut(s) 806, 1212
MroNI GCCGGC 1 cut(s) 1535
MscI TGGCCA 2 cut(s) 806, 1212
MseI TTAA 4 cut(s) 285, 507, 1317, 1497
MslI CAYNNNNRTG 6 cut(s) 705, 800, 890, 1065, 1358, 1398
Msp20I TGGCCA 2 cut(s) 806, 1212
MspI CCGG 6 cut(s) 471, 731, 860, 993, 1114, 1536
MspR9I CCNGG 6 cut(s) 236, 472, 1010, 1076, 1115, 1473
MunI CAATTG 1 cut(s) 1573
Mva1269I GAATGC 1 cut(s) 1124
MvaI CCWGG 4 cut(s) 236, 1010, 1076, 1473
MwoI GCNNNNNNNGC 4 cut(s) 72, 736, 851, 998
NaeI GCCGGC 1 cut(s) 1537
NciI CCSGG 2 cut(s) 472, 1115
NcoI CCATGG 2 cut(s) 721, 1412
NdeII GATC 5 cut(s) 361, 448, 502, 577, 1578
NgoMIV GCCGGC 1 cut(s) 1535
NheI GCTAGC 1 cut(s) 716
NlaIII CATG 8 cut(s) 516, 549, 725, 730, 1097, 1369, 1381, 1416
NlaIV GGNNCC 6 cut(s) 145, 481, 709, 1200, 1431, 1570
NmuCI GTSAC 6 cut(s) 170, 218, 223, 385, 698, 1480
NspI RCATGY 2 cut(s) 730, 1369
NspV TTCGAA 1 cut(s) 659
OliI CACNNNNGTG 2 cut(s) 705, 1358
PaeI GCATGC 1 cut(s) 730
PctI GAATGC 1 cut(s) 1124
PdiI GCCGGC 1 cut(s) 1537
PfeI GAWTC 4 cut(s) 35, 957, 1266, 1390
PflMI CCANNNNNTGG 2 cut(s) 1015, 1208
PfoI TCCNGGA 1 cut(s) 1074
PkrI GCNGC 8 cut(s) 133, 346, 409, 712, 715, 741, 844, 1000
PleI GAGTC 7 cut(s) 89, 233, 444, 469, 705, 1073, 1430
PpsI GAGTC 7 cut(s) 89, 233, 444, 469, 705, 1073, 1430
Ppu21I YACGTR 1 cut(s) 892
Psp124BI GAGCTC 1 cut(s) 524
Psp6I CCWGG 4 cut(s) 234, 1008, 1074, 1471
PspGI CCWGG 4 cut(s) 234, 1008, 1074, 1471
PspN4I GGNNCC 6 cut(s) 145, 481, 709, 1200, 1431, 1570
PspPI GGNCC 2 cut(s) 260, 1469
PstI CTGCAG 1 cut(s) 529
PsuI RGATCY 3 cut(s) 502, 577, 1578
RsaI GTAC 6 cut(s) 190, 233, 382, 827, 1200, 1364
RsaNI GTAC 6 cut(s) 189, 232, 381, 826, 1199, 1363
RseI CAYNNNNRTG 6 cut(s) 705, 800, 890, 1065, 1358, 1398
SacI GAGCTC 1 cut(s) 524
SaqAI TTAA 4 cut(s) 285, 507, 1317, 1497
SatI GCNGC 8 cut(s) 132, 345, 408, 711, 714, 740, 843, 999
Sau3AI GATC 5 cut(s) 361, 448, 502, 577, 1578
Sau96I GGNCC 2 cut(s) 260, 1469
SchI GAGTC 7 cut(s) 90, 233, 445, 470, 706, 1073, 1430
ScrFI CCNGG 6 cut(s) 236, 472, 1010, 1076, 1115, 1473
SduI GDGCHC 2 cut(s) 524, 1554
SexAI ACCWGGT 1 cut(s) 1471
SfaNI GCATC 5 cut(s) 46, 556, 1046, 1057, 1576
SfcI CTRYAG 2 cut(s) 525, 1051
SfuI TTCGAA 1 cut(s) 659
SinI GGWCC 2 cut(s) 260, 1469
SmiMI CAYNNNNRTG 6 cut(s) 705, 800, 890, 1065, 1358, 1398
SmlI CTYRAG 1 cut(s) 605
SmoI CTYRAG 1 cut(s) 605
SpeI ACTAGT 1 cut(s) 626
SphI GCATGC 1 cut(s) 730
SsiI CCGC 9 cut(s) 132, 147, 198, 688, 711, 754, 781, 998, 1013
SspMI CTAG 4 cut(s) 252, 627, 717, 1590
SstI GAGCTC 1 cut(s) 524
StyD4I CCNGG 6 cut(s) 234, 470, 1008, 1074, 1113, 1471
StyI CCWWGG 6 cut(s) 444, 483, 721, 1194, 1337, 1412
TaiI ACGT 3 cut(s) 225, 894, 1092
TaqI TCGA 6 cut(s) 489, 659, 912, 922, 1028, 1245
TatI WGTACW 2 cut(s) 825, 1362
TauI GCSGC 3 cut(s) 134, 713, 1001
TfiI GAWTC 4 cut(s) 35, 957, 1266, 1390
Tru1I TTAA 4 cut(s) 285, 507, 1317, 1497
Tru9I TTAA 4 cut(s) 285, 507, 1317, 1497
TscAI CASTG 3 cut(s) 37, 852, 1365
TseFI GTSAC 6 cut(s) 170, 218, 223, 385, 698, 1480
TseI GCWGC 5 cut(s) 344, 407, 713, 739, 842
Tsp45I GTSAC 6 cut(s) 170, 218, 223, 385, 698, 1480
TspDTI ATGAA 3 cut(s) 1082, 1135, 1366
TspGWI ACGGA 4 cut(s) 193, 391, 404, 1146
TspRI CASTG 3 cut(s) 37, 852, 1365
Van91I CCANNNNNTGG 2 cut(s) 1015, 1208
VneI GTGCAC 1 cut(s) 1550
VpaK11BI GGWCC 2 cut(s) 260, 1469
XapI RAATTY 5 cut(s) 354, 978, 1454, 1462, 1541
XceI RCATGY 2 cut(s) 730, 1369
XcmI CCANNNNNNNNNTGG 2 cut(s) 799, 1201
XspI CTAG 4 cut(s) 252, 627, 717, 1590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.