AT5G05480

Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0017136
Physical Location & Seq
Reverse (-)
1622172 .. 1624270
2099 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G05480.1

Sequence Viewer

Length: 1827 bp
ATGCCAGAAAATCAAAACGGCGCCGTTAAGTTCTTCTTCACACTTGCTTTTCTCACTGCTACCGCCGTTTCTCGTCCTTCCTCGCCGGATCGATTCCTTAGATCGGCTTCGACTTGTCTCCGGTCACCGGTCGAGCCTCAGGAGTACGAGGAGCTCAGGCGGCCCCTACCGTCCGATCAGCTAACGCCGTCATGCTCGCATGTCCTCTTCCGCCATTCATTCGCCAACACCATCAACAGACCTCCTTTTACCACTCCATACACTCCCCCTTCCAGCTGCATATCGCCGCCGTGGTCTTATGTTGTACTTGACCTACGCGCCGCCTCGAGTGGCGATCAGTACGATCGTATCTCCGGCTTGTGGCTCGGCGGAGTGGAGCTACTTCGCACCAGTACGGCGGAGCCGAGTCCATCCGGAATCTTCTGGAATGTTCGGAAAGACGTCTCTAGGTATTCCTCGCTCTTTATGAGATCCGATTTAAATGTCACAATGATGCTTGAGAACATAGTCAACGATGTTTACACAGGCATCTATCATATCAATGTCACCCTCATCTTCTACGAATTCAATCCTATTGCGTCAAATTTGAAGATTAGTAACAGATTAGGGTTTGTGGATTCCCAGAAAAGAGATCAAACCCCTGCGGATTTGATAATTCCGGTATGTGACGGAGGGAACAGAGGGTTCTGGTTCATGATCGAGAACCCCAGAGAAACTTACTCAAAAGGAATTCAAATTCCGTCCAACACTCGTCAGATTGTGCTGGAACTATATGTATCGTTCCATGGAAACGATGAGTTCTGGTATTCAAACCCGCCAAATTCTTATATCAGAACGAACAATCTAACAACCGGGCGTGGCAATGGTGCATACCGGGAAGTTTTTGTTAAGATAGATGGGAGATACGTGGGATCCGAGGTGCCATTTCCGGTGATCTTTACTGGTGGAATCAATCCCTTGTTCTGGGAACCAGTTGTAGCAATCGGTGCCTTCAATCTACCTTCCTATGACATGGACTTGACGCCATTTCTCGGGTTGCTTTTAGATGGAAAGTCTCATGAGTTTGCGTTTGGGGTCAACGATGGGATATCATACTGGCTTGTGGACGCTAATTTGCACCTCTGGTTAGATCACGGGTCTTCGAATGTTGAAGCTGGGTCTAGACTTTATGATAGCCCGAGTCGGCATATGATGAGACAAGAACAGCTTGAACAGCTTGATGGGTCGTTTAAGGTTGAAGCAGAGGTAAGTAGTGAATATGATGGCTGGGTTAGATCAAGTAAAGGTAATCTGACCACCATGGTCAAGAGCATGTTCAAGGTTGACAGCTTGGTGAGGTTCGAGAAAAACGGAACATACAAGAGGGTTGAGCAGAGAGTGAAGACCGAACGGATCGTGGAGGTGACTTCTGAATCGGGTAAACCGGTGAATCGAGTCGTGCACCAGCGGACATATCCTCTTACAGTGATCACTTCTACTCTTAGAGGGTTAACCAACGACAAGGATACGTATATGCTCGTGACGAATGTTTCACACGCCTTGAATGAGACACATTCAGATGGAGAGGCTTTGATCCAAGTTTATAGCAGACAAGACTCAGAGGGTTGGATGGAGGTTGAAGATCACAATGTCTTGGCTGGTGAAGCGAGCACAAAACAGAGTCTTAGCTATATCGATGAATTCAGCTGTTATTCCCGCACCATCGTTGCGGCTAATGGGGAGATTGCCCAGGACAGTTCATCTGATTCTTGTACTTCGTCTTCATTCTCAGAGCTCAACACTATTGAGCAGCTTATCATAACCAATGTTATGAAACACTTGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

608

Amino Acids

68.43

Weight (kDa)

5.37

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNGaseA PF12222 59 - 390 1.1e-96 Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05480
fragaria_vesca FvH4_7g24990
prunus_persica Prupe.2G255000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0370891
rosa_laevigata RLG00000027001
rosa_multiflora Rmu_co8367915.1_g000001
rosa_roxburghii Rroxscaffold_4G00286150
rosa_rugosa Rorug01G0360200
rosa_samantha Rh1AG370000 Rh1BG333600 Rh1CG346900 Rh1DG364800
rosa_wichuraiana Rw1G032560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 444
AccB1I GGYRCC 3 cut(s) 20, 919, 986
AccII CGCG 1 cut(s) 318
AccIII TCCGGA 1 cut(s) 413
AclWI GGATC 6 cut(s) 96, 465, 906, 919, 1400, 1567
AcsI RAATTY 6 cut(s) 563, 583, 729, 735, 820, 1679
AcyI GRCGYC 3 cut(s) 21, 441, 1022
AfaI GTAC 5 cut(s) 146, 306, 341, 394, 1753
AfiI CCNNNNNNNGG 5 cut(s) 103, 127, 360, 963, 1031
AgeI ACCGGT 2 cut(s) 127, 1423
AjnI CCWGG 1 cut(s) 1728
Alw21I GWGCWC 4 cut(s) 156, 1443, 1652, 1776
Alw26I GTCTC 5 cut(s) 122, 448, 1059, 1189, 1541
Alw44I GTGCAC 1 cut(s) 1439
AlwI GGATC 6 cut(s) 96, 465, 906, 919, 1400, 1567
Ama87I CYCGRG 3 cut(s) 325, 1031, 1177
Aor13HI TCCGGA 1 cut(s) 413
AoxI GGCC 1 cut(s) 161
ApaLI GTGCAC 1 cut(s) 1439
ApeKI GCWGC 2 cut(s) 276, 1789
ApoI RAATTY 6 cut(s) 563, 583, 729, 735, 820, 1679
AsiGI ACCGGT 2 cut(s) 127, 1423
AspLEI GCGC 2 cut(s) 23, 320
AspS9I GGNCC 1 cut(s) 162
AsuC2I CCSGG 2 cut(s) 853, 875
AsuHPI GGTGA 7 cut(s) 117, 538, 943, 1345, 1414, 1438, 1652
AsuII TTCGAA 1 cut(s) 1142
AvaI CYCGRG 3 cut(s) 325, 1031, 1177
AxyI CCTNAGG 1 cut(s) 138
BaeGI GKGCMC 1 cut(s) 1443
BaeI ACNNNNGTAYC 2 cut(s) 331, 364
BamHI GGATCC 1 cut(s) 911
BanI GGYRCC 3 cut(s) 20, 919, 986
BanII GRGCYC 2 cut(s) 156, 1776
BarI GAAGNNNNNNTAC 2 cut(s) 1744, 1776
BauI CACGAG 1 cut(s) 1517
BbsI GAAGAC 3 cut(s) 1131, 1388, 1752
Bbv12I GWGCWC 4 cut(s) 156, 1443, 1652, 1776
BbvI GCAGC 2 cut(s) 263, 1801
BceAI ACGGC 6 cut(s) 8, 34, 50, 172, 274, 411
BciT130I CCWGG 1 cut(s) 1730
BciVI GTATCC 1 cut(s) 1498
BclI TGATCA 1 cut(s) 1467
BcnI CCSGG 2 cut(s) 853, 875
BcoDI GTCTC 5 cut(s) 122, 448, 1059, 1189, 1541
BfaI CTAG 2 cut(s) 447, 1161
BfoI RGCGCY 1 cut(s) 24
BfuI GTATCC 1 cut(s) 1498
BisI GCNGC 6 cut(s) 161, 277, 287, 321, 1710, 1790
BlsI GCNGC 6 cut(s) 162, 278, 288, 322, 1711, 1791
Bme1390I CCNGG 3 cut(s) 853, 875, 1730
BmeT110I CYCGRG 3 cut(s) 325, 1031, 1177
BmgT120I GGNCC 1 cut(s) 162
BmiI GGNNCC 7 cut(s) 22, 164, 402, 913, 921, 969, 988
BmrFI CCNGG 3 cut(s) 853, 875, 1730
BmsI GCATC 2 cut(s) 483, 537
BpiI GAAGAC 3 cut(s) 1131, 1388, 1752
Bpu10I CCTNAGC 1 cut(s) 155
Bpu14I TTCGAA 1 cut(s) 1142
BpuEI CTTGAG 1 cut(s) 518
BpuMI CCSGG 2 cut(s) 853, 875
Bsa29I ATCGAT 2 cut(s) 91, 1674
BsaAI YACGTR 2 cut(s) 907, 1509
BsaHI GRCGYC 3 cut(s) 21, 441, 1022
BsaJI CCNNGG 5 cut(s) 290, 784, 915, 1299, 1728
BsaWI WCCGGW 6 cut(s) 120, 127, 413, 658, 928, 1423
Bsc4I CCNNNNNNNGG 5 cut(s) 103, 127, 360, 963, 1031
Bse118I RCCGGY 2 cut(s) 127, 1423
Bse1I ACTGG 4 cut(s) 390, 946, 971, 1100
Bse21I CCTNAGG 1 cut(s) 138
Bse3DI GCAATG 1 cut(s) 868
BseAI TCCGGA 1 cut(s) 413
BseBI CCWGG 1 cut(s) 1730
BseCI ATCGAT 2 cut(s) 91, 1674
BseDI CCNNGG 5 cut(s) 290, 784, 915, 1299, 1728
BseGI GGATG 2 cut(s) 410, 1614
BseLI CCNNNNNNNGG 5 cut(s) 103, 127, 360, 963, 1031
BseMI GCAATG 1 cut(s) 868
BseMII CTCAG 4 cut(s) 152, 169, 1611, 1782
BseNI ACTGG 4 cut(s) 390, 946, 971, 1100
BseRI GAGGAG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 1443
BseXI GCAGC 2 cut(s) 263, 1801
BseYI CCCAGC 2 cut(s) 1154, 1266
Bsh1236I CGCG 1 cut(s) 318
Bsh1285I CGRYCG 2 cut(s) 132, 346
BshFI GGCC 1 cut(s) 163
BshNI GGYRCC 3 cut(s) 20, 919, 986
BshTI ACCGGT 2 cut(s) 127, 1423
BshVI ATCGAT 2 cut(s) 91, 1674
BsiEI CGRYCG 2 cut(s) 132, 346
BsiHKAI GWGCWC 4 cut(s) 156, 1443, 1652, 1776
BsiHKCI CYCGRG 3 cut(s) 325, 1031, 1177
BslI CCNNNNNNNGG 5 cut(s) 103, 127, 360, 963, 1031
BsmAI GTCTC 5 cut(s) 122, 448, 1059, 1189, 1541
BsmBI CGTCTC 1 cut(s) 448
BsnI GGCC 1 cut(s) 163
BsoBI CYCGRG 3 cut(s) 325, 1031, 1177
Bsp119I TTCGAA 1 cut(s) 1142
Bsp1286I GDGCHC 4 cut(s) 156, 1443, 1652, 1776
Bsp13I TCCGGA 1 cut(s) 413
Bsp19I CCATGG 2 cut(s) 784, 1299
BspANI GGCC 1 cut(s) 163
BspCNI CTCAG 4 cut(s) 151, 168, 1610, 1781
BspDI ATCGAT 2 cut(s) 91, 1674
BspEI TCCGGA 1 cut(s) 413
BspFNI CGCG 1 cut(s) 318
BspHI TCATGA 2 cut(s) 693, 1057
BspLI GGNNCC 7 cut(s) 22, 164, 402, 913, 921, 969, 988
BspPI GGATC 6 cut(s) 96, 465, 906, 919, 1400, 1567
BspT104I TTCGAA 1 cut(s) 1142
BspT107I GGYRCC 3 cut(s) 20, 919, 986
BsrDI GCAATG 1 cut(s) 868
BsrFI RCCGGY 2 cut(s) 127, 1423
BsrI ACTGG 4 cut(s) 390, 946, 971, 1100
BssAI RCCGGY 2 cut(s) 127, 1423
BssECI CCNNGG 5 cut(s) 290, 784, 915, 1299, 1728
BssNI GRCGYC 3 cut(s) 21, 441, 1022
BssSI CACGAG 1 cut(s) 1517
BssT1I CCWWGG 2 cut(s) 784, 1299
Bst2BI CACGAG 1 cut(s) 1517
Bst2UI CCWGG 1 cut(s) 1730
Bst4CI ACNGT 3 cut(s) 171, 1465, 1736
Bst6I CTCTTC 1 cut(s) 212
BstACI GRCGYC 3 cut(s) 21, 441, 1022
BstAPI GCANNNNNTGC 1 cut(s) 986
BstBAI YACGTR 2 cut(s) 907, 1509
BstBI TTCGAA 1 cut(s) 1142
BstC8I GCNNGC 2 cut(s) 197, 1648
BstDEI CTNAG 7 cut(s) 98, 138, 155, 1481, 1597, 1664, 1768
BstDSI CCRYGG 3 cut(s) 290, 784, 1299
BstEII GGTNACC 1 cut(s) 123
BstF5I GGATG 2 cut(s) 410, 1614
BstFNI CGCG 1 cut(s) 318
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 2 cut(s) 23, 320
BstMAI GTCTC 5 cut(s) 122, 448, 1059, 1189, 1541
BstMCI CGRYCG 2 cut(s) 132, 346
BstMWI GCNNNNNNNGC 4 cut(s) 160, 986, 1213, 1643
BstNI CCWGG 1 cut(s) 1730
BstNSI RCATGY 2 cut(s) 203, 1315
BstPI GGTNACC 1 cut(s) 123
BstSCI CCNGG 3 cut(s) 851, 873, 1728
BstSLI GKGCMC 1 cut(s) 1443
BstSNI TACGTA 1 cut(s) 1509
BstUI CGCG 1 cut(s) 318
BstV1I GCAGC 2 cut(s) 263, 1801
BstV2I GAAGAC 3 cut(s) 1131, 1388, 1752
BstX2I RGATCY 2 cut(s) 470, 911
BstYI RGATCY 2 cut(s) 470, 911
Bsu15I ATCGAT 2 cut(s) 91, 1674
Bsu36I CCTNAGG 1 cut(s) 138
BsuI GTATCC 1 cut(s) 1498
BsuRI GGCC 1 cut(s) 163
BsuTUI ATCGAT 2 cut(s) 91, 1674
BtgI CCRYGG 3 cut(s) 290, 784, 1299
BtsCI GGATG 2 cut(s) 410, 1614
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 54, 1470
Cac8I GCNNGC 2 cut(s) 197, 1648
CciI TCATGA 2 cut(s) 693, 1057
CfoI GCGC 2 cut(s) 23, 320
Cfr10I RCCGGY 2 cut(s) 127, 1423
Cfr13I GGNCC 1 cut(s) 162
ClaI ATCGAT 2 cut(s) 91, 1674
CseI GACGC 3 cut(s) 567, 1030, 1115
Csp6I GTAC 5 cut(s) 145, 305, 340, 393, 1752
CspAI ACCGGT 2 cut(s) 127, 1423
CviAII CATG 8 cut(s) 192, 200, 694, 785, 1012, 1058, 1300, 1312
CviQI GTAC 5 cut(s) 145, 305, 340, 393, 1752
DdeI CTNAG 7 cut(s) 98, 138, 155, 1481, 1597, 1664, 1768
DinI GGCGCC 1 cut(s) 22
DraI TTTAAA 1 cut(s) 480
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
EciI GGCGGA 3 cut(s) 200, 384, 413
Ecl136II GAGCTC 2 cut(s) 154, 1774
Eco105I TACGTA 1 cut(s) 1509
Eco130I CCWWGG 2 cut(s) 784, 1299
Eco24I GRGCYC 2 cut(s) 156, 1776
Eco32I GATATC 1 cut(s) 1089
Eco53kI GAGCTC 2 cut(s) 154, 1774
Eco81I CCTNAGG 1 cut(s) 138
Eco88I CYCGRG 3 cut(s) 325, 1031, 1177
Eco91I GGTNACC 1 cut(s) 123
EcoICRI GAGCTC 2 cut(s) 154, 1774
EcoO65I GGTNACC 1 cut(s) 123
EcoRI GAATTC 3 cut(s) 563, 729, 1679
EcoRII CCWGG 1 cut(s) 1728
EcoRV GATATC 1 cut(s) 1089
EcoT14I CCWWGG 2 cut(s) 784, 1299
EcoT38I GRGCYC 2 cut(s) 156, 1776
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
ErhI CCWWGG 2 cut(s) 784, 1299
Esp3I CGTCTC 1 cut(s) 448
FaeI CATG 8 cut(s) 195, 203, 697, 788, 1015, 1061, 1303, 1315
FalI AAGNNNNNCTT 4 cut(s) 20, 52, 1191, 1223
FatI CATG 8 cut(s) 191, 199, 693, 784, 1011, 1057, 1299, 1311
FauI CCCGC 2 cut(s) 822, 1703
FauNDI CATATG 1 cut(s) 1188
FbaI TGATCA 1 cut(s) 1467
Fnu4HI GCNGC 6 cut(s) 161, 277, 287, 321, 1710, 1790
FokI GGATG 2 cut(s) 397, 1621
FriOI GRGCYC 2 cut(s) 156, 1776
Fsp4HI GCNGC 6 cut(s) 161, 277, 287, 321, 1710, 1790
FspBI CTAG 2 cut(s) 447, 1161
GlaI GCGC 2 cut(s) 22, 319
GluI GCNGC 6 cut(s) 161, 277, 287, 321, 1710, 1790
GsaI CCCAGC 2 cut(s) 1158, 1270
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 1 cut(s) 163
HgaI GACGC 3 cut(s) 567, 1030, 1115
HhaI GCGC 2 cut(s) 23, 320
Hin1I GRCGYC 3 cut(s) 21, 441, 1022
Hin1II CATG 8 cut(s) 195, 203, 697, 788, 1015, 1061, 1303, 1315
Hin6I GCGC 2 cut(s) 21, 318
HinP1I GCGC 2 cut(s) 21, 318
HincII GTYRAC 4 cut(s) 511, 1078, 1324, 1491
HindII GTYRAC 4 cut(s) 511, 1078, 1324, 1491
HpaI GTTAAC 1 cut(s) 1491
HphI GGTGA 7 cut(s) 117, 538, 943, 1345, 1414, 1438, 1652
Hpy166II GTNNAC 8 cut(s) 511, 520, 1078, 1105, 1324, 1421, 1441, 1491
Hpy8I GTNNAC 8 cut(s) 511, 520, 1078, 1105, 1324, 1421, 1441, 1491
HpyAV CCTTC 4 cut(s) 87, 279, 1000, 1011
HpyCH4III ACNGT 3 cut(s) 171, 1465, 1736
HpyCH4IV ACGT 3 cut(s) 441, 906, 1508
HpyCH4V TGCA 4 cut(s) 279, 869, 1117, 1441
HpyF10VI GCNNNNNNNGC 4 cut(s) 160, 986, 1213, 1643
HpyF3I CTNAG 7 cut(s) 98, 138, 155, 1481, 1597, 1664, 1768
HpySE526I ACGT 3 cut(s) 441, 906, 1508
Hsp92I GRCGYC 3 cut(s) 21, 441, 1022
Hsp92II CATG 8 cut(s) 195, 203, 697, 788, 1015, 1061, 1303, 1315
HspAI GCGC 2 cut(s) 21, 318
KasI GGCGCC 1 cut(s) 20
Kpn2I TCCGGA 1 cut(s) 413
Ksp22I TGATCA 1 cut(s) 1467
KspAI GTTAAC 1 cut(s) 1491
LmnI GCTCC 3 cut(s) 151, 376, 400
Lsp1109I GCAGC 2 cut(s) 263, 1801
LweI GCATC 2 cut(s) 483, 537
MaeI CTAG 2 cut(s) 447, 1161
MaeII ACGT 3 cut(s) 441, 906, 1508
MaeIII GTNAC 7 cut(s) 123, 484, 544, 596, 665, 1402, 1519
MflI RGATCY 2 cut(s) 470, 911
MhlI GDGCHC 4 cut(s) 156, 1443, 1652, 1776
MluCI AATT 8 cut(s) 563, 583, 654, 729, 735, 820, 1111, 1679
Mly113I GGCGCC 1 cut(s) 21
MlyI GAGTC 5 cut(s) 415, 1189, 1443, 1589, 1669
MmeI TCCRAC 2 cut(s) 768, 1586
MroI TCCGGA 1 cut(s) 413
MseI TTAA 5 cut(s) 27, 479, 888, 1230, 1490
MslI CAYNNNNRTG 3 cut(s) 491, 540, 1557
MspA1I CMGCKG 3 cut(s) 276, 1447, 1686
MspR9I CCNGG 3 cut(s) 853, 875, 1730
MvaI CCWGG 1 cut(s) 1730
MvnI CGCG 1 cut(s) 318
MwoI GCNNNNNNNGC 4 cut(s) 160, 986, 1213, 1643
NarI GGCGCC 1 cut(s) 21
NciI CCSGG 2 cut(s) 853, 875
NcoI CCATGG 2 cut(s) 784, 1299
NdeI CATATG 1 cut(s) 1188
NlaIII CATG 8 cut(s) 195, 203, 697, 788, 1015, 1061, 1303, 1315
NlaIV GGNNCC 7 cut(s) 22, 164, 402, 913, 921, 969, 988
NmeAIII GCCGAG 2 cut(s) 345, 429
NmuCI GTSAC 6 cut(s) 123, 484, 544, 665, 1402, 1519
NspI RCATGY 2 cut(s) 203, 1315
NspV TTCGAA 1 cut(s) 1142
PaeR7I CTCGAG 1 cut(s) 325
PagI TCATGA 2 cut(s) 693, 1057
PcsI WCGNNNNNNNCGW 2 cut(s) 401, 912
PfeI GAWTC 7 cut(s) 93, 417, 617, 948, 1412, 1429, 1745
PinAI ACCGGT 2 cut(s) 127, 1423
PkrI GCNGC 6 cut(s) 162, 278, 288, 322, 1711, 1791
Ple19I CGATCG 1 cut(s) 346
PleI GAGTC 5 cut(s) 414, 1188, 1442, 1589, 1668
PluTI GGCGCC 1 cut(s) 24
PpsI GAGTC 5 cut(s) 414, 1188, 1442, 1589, 1668
Ppu21I YACGTR 2 cut(s) 907, 1509
Psp124BI GAGCTC 2 cut(s) 156, 1776
Psp6I CCWGG 1 cut(s) 1728
PspEI GGTNACC 1 cut(s) 123
PspFI CCCAGC 2 cut(s) 1154, 1266
PspGI CCWGG 1 cut(s) 1728
PspN4I GGNNCC 7 cut(s) 22, 164, 402, 913, 921, 969, 988
PspPI GGNCC 1 cut(s) 162
PspXI VCTCGAGB 1 cut(s) 325
PsuI RGATCY 2 cut(s) 470, 911
PvuI CGATCG 1 cut(s) 346
PvuII CAGCTG 2 cut(s) 276, 1686
RsaI GTAC 5 cut(s) 146, 306, 341, 394, 1753
RsaNI GTAC 5 cut(s) 145, 305, 340, 393, 1752
RseI CAYNNNNRTG 3 cut(s) 491, 540, 1557
SacI GAGCTC 2 cut(s) 156, 1776
SaqAI TTAA 5 cut(s) 27, 479, 888, 1230, 1490
SatI GCNGC 6 cut(s) 161, 277, 287, 321, 1710, 1790
Sau96I GGNCC 1 cut(s) 162
SchI GAGTC 5 cut(s) 415, 1189, 1443, 1589, 1669
ScrFI CCNGG 3 cut(s) 853, 875, 1730
SduI GDGCHC 4 cut(s) 156, 1443, 1652, 1776
SfaNI GCATC 2 cut(s) 483, 537
SfoI GGCGCC 1 cut(s) 22
Sfr274I CTCGAG 1 cut(s) 325
SfuI TTCGAA 1 cut(s) 1142
SlaI CTCGAG 1 cut(s) 325
SmiI ATTTAAAT 1 cut(s) 480
SmiMI CAYNNNNRTG 3 cut(s) 491, 540, 1557
SmlI CTYRAG 2 cut(s) 325, 497
SmoI CTYRAG 2 cut(s) 325, 497
SnaBI TACGTA 1 cut(s) 1509
Sse9I AATT 8 cut(s) 563, 583, 654, 729, 735, 820, 1111, 1679
SspDI GGCGCC 1 cut(s) 20
SspMI CTAG 2 cut(s) 447, 1161
SstI GAGCTC 2 cut(s) 156, 1776
StyD4I CCNGG 3 cut(s) 851, 873, 1728
StyI CCWWGG 2 cut(s) 784, 1299
SwaI ATTTAAAT 1 cut(s) 480
TaaI ACNGT 3 cut(s) 171, 1465, 1736
TaiI ACGT 3 cut(s) 444, 909, 1511
TaqI TCGA 9 cut(s) 91, 110, 132, 326, 699, 1142, 1341, 1432, 1674
TaqII GACCGA 1 cut(s) 1400
TasI AATT 8 cut(s) 563, 583, 654, 729, 735, 820, 1111, 1679
TatI WGTACW 2 cut(s) 304, 1751
TauI GCSGC 4 cut(s) 163, 289, 323, 1712
TfiI GAWTC 7 cut(s) 93, 417, 617, 948, 1412, 1429, 1745
Tru1I TTAA 5 cut(s) 27, 479, 888, 1230, 1490
Tru9I TTAA 5 cut(s) 27, 479, 888, 1230, 1490
TscAI CASTG 2 cut(s) 61, 1470
TseFI GTSAC 6 cut(s) 123, 484, 544, 665, 1402, 1519
TseI GCWGC 2 cut(s) 276, 1789
Tsp45I GTSAC 6 cut(s) 123, 484, 544, 665, 1402, 1519
TspDTI ATGAA 6 cut(s) 207, 682, 1692, 1728, 1752, 1826
TspGWI ACGGA 4 cut(s) 684, 729, 1365, 1405
TspRI CASTG 2 cut(s) 61, 1470
VneI GTGCAC 1 cut(s) 1439
XapI RAATTY 6 cut(s) 563, 583, 729, 735, 820, 1679
XbaI TCTAGA 1 cut(s) 1160
XceI RCATGY 2 cut(s) 203, 1315
XhoI CTCGAG 1 cut(s) 325
XspI CTAG 2 cut(s) 447, 1161
ZraI GACGTC 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.