Prupe.2G255000_v2.0.a1

amidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0017136
Physical Location & Seq
Forward (+)
26771217 .. 26772962
1746 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G255000.1

Sequence Viewer

Length: 1746 bp
ATGTACGGCGTACTCCTCCTGCTTCTCCTGTTCTTCACAAACCCAGCCTCACCTTCCTCCTTCCCAGATCGTTTTACCAAATCCCTATCTGCCAATCTTCTGAAACACAAGTCCCAAGAATACCTCGAGCTCGGCCATCCCCTACCGTTCGATCAGCTGACCCCATCCTGCTCCCACCGAGTCCTCAGGCACTCCTTCGCCAACACCATGAATAGCCCTCCCTTCTCCACCGGCTACTCCCCGCCGTCCGATTGCCCATCTCCCTGGTCCCACATCGGCCTCGAGTTTCGCGCCAAATGCAAAGGCGAGCAGTACGACCGGATAGTCGGCTTGTGGCTTGGCGGCGCGGAGCTCCTCCGCACCAGCACGGCCGAGCCGACAGAAAAAGGAATCTTCTGGAAGGTTCGAAAGGACATCACCAGATACTCCTCTCTCCTCGCGCGCTGCCGCCTCAACCTCACGATGATGCTCGAGAACGTCGTTGACCACGTCTACACCGGCGTTTACCACGTCGAAGTCCATTTCCTTTATTACAACCGAAACGTCGCCGTTACGCTTAACCCCGTTATGAGGATTGCTTCGATAATCCCATATCAAAATTTGGGAACTGTTGCTTACGAACCGGCCGATTTGATCTTACCGATATCCGATAACGGAGATAAAGGATGCTGGTTCCGAATCGAGAGCGAATCGGATTTCCACTCCAAGGAAATCCGGATTCCACGGAACACACGACGACTCGTACTGGAGCTGTACGTGTCGTTTCACGGGAACGACGAGTTTTGGTACTCCAACCCGCCAAATTTATACATCACAACGAACAACTTAGCGACCGGGCGCGGCAATGGAGCTTACAGAGAGGTTTTCGTGACAGTCGACGGCGAAATGGTGGGTTCGGAAGTTCCATTTCCAGTGGTGTTCACCGGTGGCATCAACCCTCTGTTTTGGGAACCGGTGGTGGCCATCGGAGCCTTCGACCTTCCTTCGTACGATTTGGAATTGACGCCATTTCTGGAGAAGCTTCTAGATGGCAAAGCTCATAGCTTTGGAATTGGAGTGGCTGATGGGATTTCATATTGGCTTGTGGATGCCAATTTGCACATTTGGTTGGACCATCAATCAAAAAAGGTGAAGGCCAAATCAAGCGTTTTGCCTCTGCCTCCTGCTATTGAAGTGAATCGAGGTACCCAGTTTAAACAGCTTGATGGGGTTTTCAAGATCAGAGCAGAGAGAAGCAGTGAGTTTGTGGGATGGGTTAAGTCCAGTTCAGGCAACTACACCACCACATTCTCCCAGCGGTACAATTTCAAGAGCTCGATTCGATTCGCAAAGAACGGGACTTATAAATTGGTGAAGCAGAAAGTCAAGGCCAAAAGGGAAGTGAAGGTTGAGAATGAGAAAGGTGCGGTGGTGCTTAGGACCAGAGTTAGGAGGAGGTACCCTATCAATGTCATCACTGCTACTTTACCTGGGTCTCACAAGGACACTTATGTGTTAGTCACAAACATGTCGCATTCCATGAAGGAGAAGAGCTTGCATGGAGACTATTCGAGTCAGGTTTATAACAGTCAGGATTCGAGAGGGTGGATGGAGGTGAAGGATCACTCTGTTCTCTCTGGAACTGCAAATACTGAGCAGAGTTTTCGTTACAGTGGTGAGTTTGGTTGTTATTCAAGAACTGTTGGTACAAGTGATGGCAACCTCATCAGGGATAACTCCACCTTCCGTTGTTTGTCTGTCTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

582

Amino Acids

65.63

Weight (kDa)

9.08

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05480
fragaria_vesca FvH4_7g24990
prunus_persica Prupe.2G255000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0370891
rosa_laevigata RLG00000027001
rosa_multiflora Rmu_co8367915.1_g000001
rosa_roxburghii Rroxscaffold_4G00286150
rosa_rugosa Rorug01G0360200
rosa_samantha Rh1AG370000 Rh1BG333600 Rh1CG346900 Rh1DG364800
rosa_wichuraiana Rw1G032560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1344, 1563
AasI GACNNNNNNGTC 1 cut(s) 323
Acc65I GGTACC 2 cut(s) 1184, 1437
AccB1I GGYRCC 2 cut(s) 1184, 1437
AccI GTMKAC 2 cut(s) 492, 876
AccII CGCG 5 cut(s) 291, 347, 440, 442, 840
AccIII TCCGGA 1 cut(s) 714
AciI CCGC 9 cut(s) 242, 342, 347, 358, 448, 797, 840, 1297, 1406
AclWI GGATC 1 cut(s) 1608
AcoI YGGCCR 4 cut(s) 133, 369, 624, 960
AcsI RAATTY 2 cut(s) 598, 802
AcyI GRCGYC 1 cut(s) 1004
AfiI CCNNNNNNNGG 4 cut(s) 570, 706, 1428, 1708
AflIII ACRYGT 2 cut(s) 756, 1506
AgeI ACCGGT 2 cut(s) 923, 952
AgsI TTSAA 4 cut(s) 1172, 1216, 1309, 1674
AjiI CACGTC 2 cut(s) 490, 511
AjnI CCWGG 2 cut(s) 263, 1468
AleI CACNNNNGTG 1 cut(s) 1490
Alw21I GWGCWC 3 cut(s) 132, 354, 1316
Alw26I GTCTC 2 cut(s) 1479, 1536
AlwI GGATC 1 cut(s) 1608
Ama87I CYCGRG 3 cut(s) 125, 281, 470
Aor13HI TCCGGA 1 cut(s) 714
AoxI GGCC 7 cut(s) 133, 277, 369, 624, 960, 1134, 1368
ApeKI GCWGC 1 cut(s) 444
ApoI RAATTY 2 cut(s) 598, 802
AsiGI ACCGGT 2 cut(s) 923, 952
Asp718I GGTACC 2 cut(s) 1184, 1437
AspLEI GCGC 5 cut(s) 293, 347, 442, 444, 840
AspS9I GGNCC 3 cut(s) 267, 1111, 1419
AsuC2I CCSGG 1 cut(s) 835
AsuHPI GGTGA 7 cut(s) 42, 409, 913, 1141, 1363, 1606, 1667
AsuII TTCGAA 1 cut(s) 406
AvaI CYCGRG 3 cut(s) 125, 281, 470
AvaII GGWCC 3 cut(s) 267, 1111, 1419
AxyI CCTNAGG 1 cut(s) 185
BalI TGGCCA 1 cut(s) 962
BanI GGYRCC 2 cut(s) 1184, 1437
BanII GRGCYC 3 cut(s) 132, 354, 1316
Bbv12I GWGCWC 3 cut(s) 132, 354, 1316
BbvI GCAGC 1 cut(s) 431
BceAI ACGGC 5 cut(s) 22, 229, 384, 533, 895
BcgI CGANNNNNNTGC 2 cut(s) 1625, 1659
BciT130I CCWGG 2 cut(s) 265, 1470
BcnI CCSGG 1 cut(s) 835
BcoDI GTCTC 2 cut(s) 1479, 1536
BfaI CTAG 1 cut(s) 1025
BisI GCNGC 4 cut(s) 343, 445, 448, 841
BlsI GCNGC 4 cut(s) 344, 446, 449, 842
Bme1390I CCNGG 3 cut(s) 265, 835, 1470
Bme18I GGWCC 3 cut(s) 267, 1111, 1419
BmeT110I CYCGRG 3 cut(s) 125, 281, 470
BmgBI CACGTC 2 cut(s) 490, 511
BmgT120I GGNCC 3 cut(s) 267, 1111, 1419
BmiI GGNNCC 6 cut(s) 269, 674, 951, 970, 1186, 1439
BmrFI CCNGG 3 cut(s) 265, 835, 1470
BmrI ACTGGG 1 cut(s) 1183
BmsI GCATC 4 cut(s) 456, 656, 939, 1078
BmuI ACTGGG 1 cut(s) 1183
BpmI CTGGAG 2 cut(s) 767, 1034
Bpu10I CCTNAGC 1 cut(s) 1415
Bpu14I TTCGAA 1 cut(s) 406
BpuMI CCSGG 1 cut(s) 835
BsaAI YACGTR 1 cut(s) 757
BsaHI GRCGYC 1 cut(s) 1004
BsaI GGTCTC 1 cut(s) 1479
BsaJI CCNNGG 4 cut(s) 263, 705, 722, 1469
BsaWI WCCGGW 4 cut(s) 318, 714, 923, 952
Bsc4I CCNNNNNNNGG 4 cut(s) 570, 706, 1428, 1708
Bse118I RCCGGY 5 cut(s) 230, 497, 622, 923, 952
Bse1I ACTGG 4 cut(s) 750, 911, 1189, 1263
Bse21I CCTNAGG 1 cut(s) 185
Bse3DI GCAATG 1 cut(s) 850
BseAI TCCGGA 1 cut(s) 714
BseBI CCWGG 2 cut(s) 265, 1470
BseDI CCNNGG 4 cut(s) 263, 705, 722, 1469
BseGI GGATG 6 cut(s) 136, 164, 671, 1093, 1256, 1593
BseLI CCNNNNNNNGG 4 cut(s) 570, 706, 1428, 1708
BseMI GCAATG 1 cut(s) 850
BseMII CTCAG 2 cut(s) 199, 1623
BseNI ACTGG 4 cut(s) 750, 911, 1189, 1263
BsePI GCGCGC 1 cut(s) 440
BseRI GAGGAG 5 cut(s) 5, 344, 418, 425, 1447
BseX3I CGGCCG 2 cut(s) 369, 624
BseXI GCAGC 1 cut(s) 431
BseYI CCCAGC 2 cut(s) 43, 1293
Bsh1236I CGCG 5 cut(s) 291, 347, 440, 442, 840
Bsh1285I CGRYCG 4 cut(s) 319, 372, 627, 834
BshFI GGCC 7 cut(s) 135, 279, 371, 626, 962, 1136, 1370
BshNI GGYRCC 2 cut(s) 1184, 1437
BshTI ACCGGT 2 cut(s) 923, 952
BsiEI CGRYCG 4 cut(s) 319, 372, 627, 834
BsiHKAI GWGCWC 3 cut(s) 132, 354, 1316
BsiHKCI CYCGRG 3 cut(s) 125, 281, 470
BsiSI CCGG 8 cut(s) 231, 319, 498, 623, 715, 834, 924, 953
BsiWI CGTACG 1 cut(s) 987
BslFI GGGAC 3 cut(s) 97, 253, 1351
BslI CCNNNNNNNGG 4 cut(s) 570, 706, 1428, 1708
BsmAI GTCTC 2 cut(s) 1479, 1536
BsmFI GGGAC 3 cut(s) 97, 253, 1351
BsmI GAATGC 1 cut(s) 1513
BsnI GGCC 7 cut(s) 135, 279, 371, 626, 962, 1136, 1370
Bso31I GGTCTC 1 cut(s) 1479
BsoBI CYCGRG 3 cut(s) 125, 281, 470
Bsp119I TTCGAA 1 cut(s) 406
Bsp1286I GDGCHC 3 cut(s) 132, 354, 1316
Bsp13I TCCGGA 1 cut(s) 714
Bsp143I GATC 5 cut(s) 67, 151, 633, 1218, 1600
BspACI CCGC 9 cut(s) 242, 342, 347, 358, 448, 797, 840, 1297, 1406
BspANI GGCC 7 cut(s) 135, 279, 371, 626, 962, 1136, 1370
BspCNI CTCAG 2 cut(s) 198, 1624
BspEI TCCGGA 1 cut(s) 714
BspFNI CGCG 5 cut(s) 291, 347, 440, 442, 840
BspHI TCATGA 1 cut(s) 1742
BspLI GGNNCC 6 cut(s) 269, 674, 951, 970, 1186, 1439
BspPI GGATC 1 cut(s) 1608
BspQI GCTCTTC 1 cut(s) 1523
BspT104I TTCGAA 1 cut(s) 406
BspT107I GGYRCC 2 cut(s) 1184, 1437
BspTNI GGTCTC 1 cut(s) 1479
BsrDI GCAATG 1 cut(s) 850
BsrFI RCCGGY 5 cut(s) 230, 497, 622, 923, 952
BsrI ACTGG 4 cut(s) 750, 911, 1189, 1263
BssAI RCCGGY 5 cut(s) 230, 497, 622, 923, 952
BssECI CCNNGG 4 cut(s) 263, 705, 722, 1469
BssHII GCGCGC 1 cut(s) 440
BssMI GATC 5 cut(s) 67, 151, 633, 1218, 1600
BssNI GRCGYC 1 cut(s) 1004
BssT1I CCWWGG 1 cut(s) 705
Bst2UI CCWGG 2 cut(s) 265, 1470
Bst4CI ACNGT 6 cut(s) 147, 610, 874, 1568, 1652, 1681
Bst6I CTCTTC 1 cut(s) 1523
BstACI GRCGYC 1 cut(s) 1004
BstBAI YACGTR 1 cut(s) 757
BstBI TTCGAA 1 cut(s) 406
BstC8I GCNNGC 3 cut(s) 308, 442, 1535
BstDEI CTNAG 4 cut(s) 185, 826, 1415, 1632
BstDSI CCRYGG 1 cut(s) 722
BstF5I GGATG 6 cut(s) 136, 164, 671, 1093, 1256, 1593
BstFNI CGCG 5 cut(s) 291, 347, 440, 442, 840
BstHHI GCGC 5 cut(s) 293, 347, 442, 444, 840
BstKTI GATC 5 cut(s) 70, 154, 636, 1221, 1603
BstMAI GTCTC 2 cut(s) 1479, 1536
BstMBI GATC 5 cut(s) 67, 151, 633, 1218, 1600
BstMCI CGRYCG 4 cut(s) 319, 372, 627, 834
BstMWI GCNNNNNNNGC 2 cut(s) 297, 968
BstNI CCWGG 2 cut(s) 265, 1470
BstNSI RCATGY 1 cut(s) 1510
BstSCI CCNGG 3 cut(s) 263, 833, 1468
BstUI CGCG 5 cut(s) 291, 347, 440, 442, 840
BstV1I GCAGC 1 cut(s) 431
BstXI CCANNNNNNTGG 1 cut(s) 264
BstZI CGGCCG 2 cut(s) 369, 624
Bsu36I CCTNAGG 1 cut(s) 185
BsuRI GGCC 7 cut(s) 135, 279, 371, 626, 962, 1136, 1370
BtgI CCRYGG 1 cut(s) 722
BtrI CACGTC 2 cut(s) 490, 511
BtsCI GGATG 6 cut(s) 136, 164, 671, 1093, 1256, 1593
BtsI GCAGTG 2 cut(s) 1243, 1455
BtsIMutI CAGTG 4 cut(s) 918, 1243, 1455, 1657
Cac8I GCNNGC 3 cut(s) 308, 442, 1535
CciI TCATGA 1 cut(s) 1742
CfoI GCGC 5 cut(s) 293, 347, 442, 444, 840
Cfr10I RCCGGY 5 cut(s) 230, 497, 622, 923, 952
Cfr13I GGNCC 3 cut(s) 267, 1111, 1419
CseI GACGC 1 cut(s) 1012
CspAI ACCGGT 2 cut(s) 923, 952
CviAII CATG 5 cut(s) 208, 1507, 1519, 1538, 1743
DdeI CTNAG 4 cut(s) 185, 826, 1415, 1632
DpnI GATC 5 cut(s) 69, 153, 635, 1220, 1602
DpnII GATC 5 cut(s) 67, 151, 633, 1218, 1600
DraI TTTAAA 1 cut(s) 1195
DrdI GACNNNNNNGTC 1 cut(s) 323
DseDI GACNNNNNNGTC 1 cut(s) 323
EaeI YGGCCR 4 cut(s) 133, 369, 624, 960
EagI CGGCCG 2 cut(s) 369, 624
Eam1104I CTCTTC 1 cut(s) 1523
EarI CTCTTC 1 cut(s) 1523
Ecl136II GAGCTC 3 cut(s) 130, 352, 1314
EclXI CGGCCG 2 cut(s) 369, 624
Eco130I CCWWGG 1 cut(s) 705
Eco24I GRGCYC 3 cut(s) 132, 354, 1316
Eco31I GGTCTC 1 cut(s) 1479
Eco32I GATATC 1 cut(s) 645
Eco47I GGWCC 3 cut(s) 267, 1111, 1419
Eco52I CGGCCG 2 cut(s) 369, 624
Eco53kI GAGCTC 3 cut(s) 130, 352, 1314
Eco81I CCTNAGG 1 cut(s) 185
Eco88I CYCGRG 3 cut(s) 125, 281, 470
EcoICRI GAGCTC 3 cut(s) 130, 352, 1314
EcoRII CCWGG 2 cut(s) 263, 1468
EcoRV GATATC 1 cut(s) 645
EcoT14I CCWWGG 1 cut(s) 705
EcoT38I GRGCYC 3 cut(s) 132, 354, 1316
ErhI CCWWGG 1 cut(s) 705
FaeI CATG 5 cut(s) 211, 1510, 1522, 1541, 1746
FaqI GGGAC 3 cut(s) 97, 253, 1351
FatI CATG 5 cut(s) 207, 1506, 1518, 1537, 1742
FauI CCCGC 2 cut(s) 249, 804
FblI GTMKAC 2 cut(s) 492, 876
Fnu4HI GCNGC 4 cut(s) 343, 445, 448, 841
FokI GGATG 6 cut(s) 123, 151, 678, 1100, 1263, 1600
FriOI GRGCYC 3 cut(s) 132, 354, 1316
Fsp4HI GCNGC 4 cut(s) 343, 445, 448, 841
FspBI CTAG 1 cut(s) 1025
GlaI GCGC 5 cut(s) 292, 346, 441, 443, 839
GluI GCNGC 4 cut(s) 343, 445, 448, 841
GsaI CCCAGC 2 cut(s) 47, 1297
GsuI CTGGAG 2 cut(s) 767, 1034
HaeIII GGCC 7 cut(s) 135, 279, 371, 626, 962, 1136, 1370
HapII CCGG 8 cut(s) 231, 319, 498, 623, 715, 834, 924, 953
HgaI GACGC 1 cut(s) 1012
HhaI GCGC 5 cut(s) 293, 347, 442, 444, 840
Hin1I GRCGYC 1 cut(s) 1004
Hin1II CATG 5 cut(s) 211, 1510, 1522, 1541, 1746
Hin6I GCGC 5 cut(s) 291, 345, 440, 442, 838
HinP1I GCGC 5 cut(s) 291, 345, 440, 442, 838
HincII GTYRAC 2 cut(s) 484, 877
HindII GTYRAC 2 cut(s) 484, 877
HindIII AAGCTT 1 cut(s) 1019
HpaII CCGG 8 cut(s) 231, 319, 498, 623, 715, 834, 924, 953
HphI GGTGA 7 cut(s) 42, 409, 913, 1141, 1363, 1606, 1667
Hpy166II GTNNAC 5 cut(s) 484, 493, 505, 877, 921
Hpy188I TCNGA 8 cut(s) 102, 250, 649, 677, 694, 898, 968, 1223
Hpy8I GTNNAC 5 cut(s) 484, 493, 505, 877, 921
Hpy99I CGWCG 6 cut(s) 482, 515, 548, 738, 779, 881
HpyCH4III ACNGT 6 cut(s) 147, 610, 874, 1568, 1652, 1681
HpyCH4IV ACGT 5 cut(s) 477, 489, 510, 543, 756
HpyCH4V TGCA 4 cut(s) 300, 1099, 1537, 1625
HpyF10VI GCNNNNNNNGC 2 cut(s) 297, 968
HpyF3I CTNAG 4 cut(s) 185, 826, 1415, 1632
HpySE526I ACGT 5 cut(s) 477, 489, 510, 543, 756
Hsp92I GRCGYC 1 cut(s) 1004
Hsp92II CATG 5 cut(s) 211, 1510, 1522, 1541, 1746
HspAI GCGC 5 cut(s) 291, 345, 440, 442, 838
Kpn2I TCCGGA 1 cut(s) 714
KpnI GGTACC 2 cut(s) 1188, 1441
Kzo9I GATC 5 cut(s) 67, 151, 633, 1218, 1600
LguI GCTCTTC 1 cut(s) 1523
LmnI GCTCC 6 cut(s) 176, 349, 357, 748, 848, 968
Lsp1109I GCAGC 1 cut(s) 431
LweI GCATC 4 cut(s) 456, 656, 939, 1078
MaeI CTAG 1 cut(s) 1025
MaeII ACGT 5 cut(s) 477, 489, 510, 543, 756
MaeIII GTNAC 4 cut(s) 550, 868, 1498, 1646
MalI GATC 5 cut(s) 69, 153, 635, 1220, 1602
MboI GATC 5 cut(s) 67, 151, 633, 1218, 1600
MboII GAAGA 4 cut(s) 25, 89, 385, 1540
MhlI GDGCHC 3 cut(s) 132, 354, 1316
MlsI TGGCCA 1 cut(s) 962
MluCI AATT 7 cut(s) 598, 802, 998, 1050, 1093, 1303, 1346
MluNI TGGCCA 1 cut(s) 962
MlyI GAGTC 3 cut(s) 189, 732, 1561
MmeI TCCRAC 2 cut(s) 816, 1089
Mox20I TGGCCA 1 cut(s) 962
MroI TCCGGA 1 cut(s) 714
MscI TGGCCA 1 cut(s) 962
MseI TTAA 3 cut(s) 558, 1194, 1257
MslI CAYNNNNRTG 3 cut(s) 464, 1490, 1505
Msp20I TGGCCA 1 cut(s) 962
MspA1I CMGCKG 2 cut(s) 157, 1297
MspI CCGG 8 cut(s) 231, 319, 498, 623, 715, 834, 924, 953
MspR9I CCNGG 3 cut(s) 265, 835, 1470
MssI GTTTAAAC 1 cut(s) 1195
Mva1269I GAATGC 1 cut(s) 1513
MvaI CCWGG 2 cut(s) 265, 1470
MvnI CGCG 5 cut(s) 291, 347, 440, 442, 840
MwoI GCNNNNNNNGC 2 cut(s) 297, 968
NciI CCSGG 1 cut(s) 835
NdeII GATC 5 cut(s) 67, 151, 633, 1218, 1600
NlaIII CATG 5 cut(s) 211, 1510, 1522, 1541, 1746
NlaIV GGNNCC 6 cut(s) 269, 674, 951, 970, 1186, 1439
NmeAIII GCCGAG 2 cut(s) 111, 397
NmuCI GTSAC 2 cut(s) 868, 1498
NspI RCATGY 1 cut(s) 1510
NspV TTCGAA 1 cut(s) 406
OliI CACNNNNGTG 1 cut(s) 1490
PaeR7I CTCGAG 3 cut(s) 125, 281, 470
PagI TCATGA 1 cut(s) 1742
PauI GCGCGC 1 cut(s) 440
PciI ACATGT 1 cut(s) 1506
PciSI GCTCTTC 1 cut(s) 1523
PcsI WCGNNNNNNNCGW 8 cut(s) 374, 477, 486, 624, 730, 774, 873, 972
PctI GAATGC 1 cut(s) 1513
PfeI GAWTC 8 cut(s) 390, 678, 689, 718, 1177, 1318, 1323, 1574
Pfl23II CGTACG 1 cut(s) 987
PflFI GACNNNGTC 1 cut(s) 488
PinAI ACCGGT 2 cut(s) 923, 952
PkrI GCNGC 4 cut(s) 344, 446, 449, 842
PleI GAGTC 3 cut(s) 188, 732, 1560
PmeI GTTTAAAC 1 cut(s) 1195
PpsI GAGTC 3 cut(s) 188, 732, 1560
Ppu21I YACGTR 1 cut(s) 757
PscI ACATGT 1 cut(s) 1506
PsiI TTATAA 2 cut(s) 1344, 1563
Psp124BI GAGCTC 3 cut(s) 132, 354, 1316
Psp6I CCWGG 2 cut(s) 263, 1468
PspFI CCCAGC 2 cut(s) 43, 1293
PspGI CCWGG 2 cut(s) 263, 1468
PspLI CGTACG 1 cut(s) 987
PspN4I GGNNCC 6 cut(s) 269, 674, 951, 970, 1186, 1439
PspPI GGNCC 3 cut(s) 267, 1111, 1419
PspXI VCTCGAGB 2 cut(s) 125, 281
PsrI GAACNNNNNNTAC 4 cut(s) 1612, 1644, 1669, 1701
PsyI GACNNNGTC 1 cut(s) 488
PteI GCGCGC 1 cut(s) 440
PvuII CAGCTG 1 cut(s) 157
RseI CAYNNNNRTG 3 cut(s) 464, 1490, 1505
SacI GAGCTC 3 cut(s) 132, 354, 1316
SalI GTCGAC 1 cut(s) 875
SapI GCTCTTC 1 cut(s) 1523
SaqAI TTAA 3 cut(s) 558, 1194, 1257
SatI GCNGC 4 cut(s) 343, 445, 448, 841
Sau3AI GATC 5 cut(s) 67, 151, 633, 1218, 1600
Sau96I GGNCC 3 cut(s) 267, 1111, 1419
SchI GAGTC 3 cut(s) 189, 732, 1561
ScrFI CCNGG 3 cut(s) 265, 835, 1470
SduI GDGCHC 3 cut(s) 132, 354, 1316
SfaNI GCATC 4 cut(s) 456, 656, 939, 1078
Sfr274I CTCGAG 3 cut(s) 125, 281, 470
SfuI TTCGAA 1 cut(s) 406
SgrAI CRCCGGYG 2 cut(s) 497, 923
SinI GGWCC 3 cut(s) 267, 1111, 1419
SlaI CTCGAG 3 cut(s) 125, 281, 470
SmiMI CAYNNNNRTG 3 cut(s) 464, 1490, 1505
SmlI CTYRAG 3 cut(s) 125, 281, 470
SmoI CTYRAG 3 cut(s) 125, 281, 470
Sse9I AATT 7 cut(s) 598, 802, 998, 1050, 1093, 1303, 1346
SsiI CCGC 9 cut(s) 242, 342, 347, 358, 448, 797, 840, 1297, 1406
SspMI CTAG 1 cut(s) 1025
SstI GAGCTC 3 cut(s) 132, 354, 1316
StyD4I CCNGG 3 cut(s) 263, 833, 1468
StyI CCWWGG 1 cut(s) 705
TaaI ACNGT 6 cut(s) 147, 610, 874, 1568, 1652, 1681
TaiI ACGT 5 cut(s) 480, 492, 513, 546, 759
TasI AATT 7 cut(s) 598, 802, 998, 1050, 1093, 1303, 1346
TauI GCSGC 3 cut(s) 345, 450, 843
TfiI GAWTC 8 cut(s) 390, 678, 689, 718, 1177, 1318, 1323, 1574
Tru1I TTAA 3 cut(s) 558, 1194, 1257
Tru9I TTAA 3 cut(s) 558, 1194, 1257
TscAI CASTG 4 cut(s) 918, 1243, 1462, 1657
TseFI GTSAC 2 cut(s) 868, 1498
TseI GCWGC 1 cut(s) 444
Tsp45I GTSAC 2 cut(s) 868, 1498
TspDTI ATGAA 3 cut(s) 224, 1062, 1535
TspGWI ACGGA 3 cut(s) 669, 739, 1715
TspRI CASTG 4 cut(s) 918, 1243, 1462, 1657
Tth111I GACNNNGTC 1 cut(s) 488
VpaK11BI GGWCC 3 cut(s) 267, 1111, 1419
XapI RAATTY 2 cut(s) 598, 802
XbaI TCTAGA 1 cut(s) 1024
XceI RCATGY 1 cut(s) 1510
XhoI CTCGAG 3 cut(s) 125, 281, 470
XmiI GTMKAC 2 cut(s) 492, 876
XspI CTAG 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.