Rh1DG364800

amidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0017136
Physical Location & Seq
Forward (+)
59060188 .. 59061474
1287 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG364800.1

Sequence Viewer

Length: 1287 bp
ATGCTCGAGAACGTCGTCGATCGCGTCTTCACCGGCGTTTACCACGTCGAGGTTCATTTCCTCTATTACAAGGCAAACGTCGCCGTTTCGGAGCCTAGGGTTTCTTCGATCCTTGCAGATCATACTTTCAACGGTCAAAAATTGGGGATCGTTGTTCCCGAGGAGCCTGCCGATCTGATCGTTCCGATCTCCGACGACGGTGACCGAGGGTTTTGGTTCAGAATCGACGGCGAATCGGATTTCCCTTCCAAGCAAATCCAACTCCCTCGGAACACACGACGTGTCGTTCTTGAGCTCTATGTTTCGTTTCACGGCAACGACGAGTTCTGGTACTCGAATCCGCCGAATCTATACATCACGACGAACAATCTTACGACCGGACGAGGAAATGGAGCTTACAGAGAGGTTTTCGTGACGGTCGACGGCGAGTTGGTGGGATCGGAGGTGCCGTTTCCGGTGGTGTTCACCGGCGGGATCAATCCGTTGTTTTGGGAACCGGTGGTGGCCATCGGAGCTTTCAACCTTCCTTCTTATGATTTCGAATTGACGCCGTTTCTGGGGAAGCTTCTGGATAAGAAGGCTCATAGCTTTGGAATTGGAGTGACCGATGGAATTTCATATTGGCTTGTTAATGCCAACTTACACATTTGGTTGGATCATAAATCCAAGGCTGTGAAAGCTAAATCAAACTCGTTTCCTTCGCCGGCGATTGAAGTGAAGCGTAATGCTCAGTTCAAAAAGCTTGACGGGTCGTTCAGGATCAAGGCAAGGAGGAGGAGTGACTTTGTGGGGTGGGTTAAGTCGAGCTCAGGGAACTACACGACCACATTCTCGCAGAAGTACAAGTTCAGCAGCTCGGTTAGTTTTGGGAAGAAGGGGACGTACAAATTGGTGAACCAGAAGGTCAAGGCTGAGAAGGAAGTGAGGGTTGTGAGTGAGATTGGTGTGGTGGTTCTTAGAACTAGGGTTAAGGGAAGGTACCCTATTGATGTTTTTACAGCTACTCTACCGGGTAAGAAGAAAGACACCTATGTGCTCCTCACCAATGTCACGCATTCGGTGAAGGAGAGGAGCTGGCACGGGGGATATTTGAGCCGTGTTAACAACATTCAAGATTCAAGAGGGTGGATGGAGGTCAGGGATCATTCTGTTCTTTCTGGAACAGCAGACACTCGGCAGAGCTTCGGTTACAAAGATAACTTTGGCTGCTACTCGCGCACTATTGGCGCTGTTGATGGCAGCTTGATCAGGGATAATTCCAGCTTTCATTGCATATCTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

428

Amino Acids

48.01

Weight (kDa)

9.36

Isoelectric Point (pI)

31.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNGaseA PF12222 2 - 230 2.2e-58 Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
PNGase_A_C PF25156 251 - 308 1e-06 PNGase_A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05480
fragaria_vesca FvH4_7g24990
prunus_persica Prupe.2G255000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0370891
rosa_laevigata RLG00000027001
rosa_multiflora Rmu_co8367915.1_g000001
rosa_roxburghii Rroxscaffold_4G00286150
rosa_rugosa Rorug01G0360200
rosa_samantha Rh1AG370000 Rh1BG333600 Rh1CG346900 Rh1DG364800
rosa_wichuraiana Rw1G032560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 978
AccB1I GGYRCC 2 cut(s) 445, 978
AccI GTMKAC 1 cut(s) 420
AccII CGCG 2 cut(s) 24, 1216
AciI CCGC 2 cut(s) 341, 471
AclWI GGATC 7 cut(s) 103, 155, 445, 482, 663, 767, 1149
AcoI YGGCCR 1 cut(s) 504
AcsI RAATTY 1 cut(s) 612
AcyI GRCGYC 1 cut(s) 548
AdeI CACNNNGTG 1 cut(s) 281
AfaI GTAC 4 cut(s) 332, 842, 884, 980
AfiI CCNNNNNNNGG 2 cut(s) 49, 557
AflIII ACRYGT 1 cut(s) 280
AgeI ACCGGT 1 cut(s) 496
AgsI TTSAA 6 cut(s) 130, 520, 713, 736, 1112, 1119
AjiI CACGTC 2 cut(s) 46, 281
AjuI GAANNNNNNNTTGG 2 cut(s) 604, 636
AleI CACNNNNGTG 1 cut(s) 1031
Alw21I GWGCWC 3 cut(s) 297, 809, 1038
AlwI GGATC 7 cut(s) 103, 155, 445, 482, 663, 767, 1149
Ama87I CYCGRG 2 cut(s) 5, 158
AoxI GGCC 1 cut(s) 504
ApeKI GCWGC 4 cut(s) 852, 1206, 1239, 1280
ApoI RAATTY 1 cut(s) 612
AsiGI ACCGGT 1 cut(s) 496
Asp718I GGTACC 1 cut(s) 978
AspA2I CCTAGG 1 cut(s) 95
AspLEI GCGC 2 cut(s) 1218, 1229
AsuC2I CCSGG 1 cut(s) 1011
AsuHPI GGTGA 6 cut(s) 22, 212, 457, 904, 1033, 1072
AsuII TTCGAA 1 cut(s) 540
AvaI CYCGRG 2 cut(s) 5, 158
AvrII CCTAGG 1 cut(s) 95
BalI TGGCCA 1 cut(s) 506
BanI GGYRCC 2 cut(s) 445, 978
BanII GRGCYC 2 cut(s) 297, 809
BarI GAAGNNNNNNTAC 2 cut(s) 866, 898
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 3 cut(s) 297, 809, 1038
BbvI GCAGC 4 cut(s) 864, 1193, 1251, 1267
BccI CCATC 4 cut(s) 515, 602, 1123, 1229
BceAI ACGGC 7 cut(s) 68, 244, 328, 433, 439, 535, 1080
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BclI TGATCA 1 cut(s) 1245
BcnI CCSGG 1 cut(s) 1011
BfaI CTAG 2 cut(s) 96, 963
BfoI RGCGCY 1 cut(s) 1230
BisI GCNGC 4 cut(s) 853, 1207, 1240, 1281
BlnI CCTAGG 1 cut(s) 95
BlsI GCNGC 4 cut(s) 854, 1208, 1241, 1282
Bme1390I CCNGG 1 cut(s) 1011
BmeT110I CYCGRG 2 cut(s) 5, 158
BmgBI CACGTC 2 cut(s) 46, 281
BmiI GGNNCC 5 cut(s) 93, 165, 447, 495, 980
BmrFI CCNGG 1 cut(s) 1011
BpiI GAAGAC 1 cut(s) 19
Bpu10I CCTNAGC 1 cut(s) 808
Bpu14I TTCGAA 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 1 cut(s) 1011
BsaHI GRCGYC 1 cut(s) 548
BsaJI CCNNGG 5 cut(s) 95, 159, 205, 266, 666
BsaWI WCCGGW 3 cut(s) 377, 454, 496
BsaXI ACNNNNNCTCC 4 cut(s) 434, 464, 763, 793
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 557
Bse118I RCCGGY 4 cut(s) 32, 467, 496, 703
Bse3DI GCAATG 1 cut(s) 1267
BseDI CCNNGG 5 cut(s) 95, 159, 205, 266, 666
BseGI GGATG 1 cut(s) 1134
BseLI CCNNNNNNNGG 2 cut(s) 49, 557
BseMI GCAATG 1 cut(s) 1267
BseMII CTCAG 3 cut(s) 743, 822, 903
BseRI GAGGAG 5 cut(s) 176, 787, 790, 1028, 1084
BseXI GCAGC 4 cut(s) 864, 1193, 1251, 1267
Bsh1236I CGCG 2 cut(s) 24, 1216
Bsh1285I CGRYCG 3 cut(s) 22, 378, 420
BshFI GGCC 1 cut(s) 506
BshNI GGYRCC 2 cut(s) 445, 978
BshTI ACCGGT 1 cut(s) 496
BsiEI CGRYCG 3 cut(s) 22, 378, 420
BsiHKAI GWGCWC 3 cut(s) 297, 809, 1038
BsiHKCI CYCGRG 2 cut(s) 5, 158
BsiSI CCGG 7 cut(s) 33, 378, 455, 468, 497, 704, 1010
BslFI GGGAC 1 cut(s) 892
BslI CCNNNNNNNGG 2 cut(s) 49, 557
BsmFI GGGAC 1 cut(s) 892
BsmI GAATGC 1 cut(s) 1054
BsnI GGCC 1 cut(s) 506
BsoBI CYCGRG 2 cut(s) 5, 158
Bsp119I TTCGAA 1 cut(s) 540
Bsp1286I GDGCHC 3 cut(s) 297, 809, 1038
BspACI CCGC 2 cut(s) 341, 471
BspANI GGCC 1 cut(s) 506
BspCNI CTCAG 3 cut(s) 742, 821, 904
BspFNI CGCG 2 cut(s) 24, 1216
BspLI GGNNCC 5 cut(s) 93, 165, 447, 495, 980
BspPI GGATC 7 cut(s) 103, 155, 445, 482, 663, 767, 1149
BspT104I TTCGAA 1 cut(s) 540
BspT107I GGYRCC 2 cut(s) 445, 978
BsrDI GCAATG 1 cut(s) 1267
BsrFI RCCGGY 4 cut(s) 32, 467, 496, 703
BssAI RCCGGY 4 cut(s) 32, 467, 496, 703
BssECI CCNNGG 5 cut(s) 95, 159, 205, 266, 666
BssNI GRCGYC 1 cut(s) 548
BssT1I CCWWGG 2 cut(s) 95, 666
Bst4CI ACNGT 3 cut(s) 134, 200, 418
BstACI GRCGYC 1 cut(s) 548
BstBI TTCGAA 1 cut(s) 540
BstC8I GCNNGC 3 cut(s) 168, 705, 1076
BstDEI CTNAG 4 cut(s) 729, 808, 912, 956
BstEII GGTNACC 1 cut(s) 200
BstF5I GGATG 1 cut(s) 1134
BstFNI CGCG 2 cut(s) 24, 1216
BstH2I RGCGCY 1 cut(s) 1230
BstHHI GCGC 2 cut(s) 1218, 1229
BstMCI CGRYCG 3 cut(s) 22, 378, 420
BstMWI GCNNNNNNNGC 6 cut(s) 80, 512, 677, 1215, 1224, 1269
BstPI GGTNACC 1 cut(s) 200
BstSCI CCNGG 1 cut(s) 1009
BstUI CGCG 2 cut(s) 24, 1216
BstV1I GCAGC 4 cut(s) 864, 1193, 1251, 1267
BstV2I GAAGAC 1 cut(s) 19
BsuRI GGCC 1 cut(s) 506
BtrI CACGTC 2 cut(s) 46, 281
BtsCI GGATG 1 cut(s) 1134
Cac8I GCNNGC 3 cut(s) 168, 705, 1076
CfoI GCGC 2 cut(s) 1218, 1229
Cfr10I RCCGGY 4 cut(s) 32, 467, 496, 703
CseI GACGC 2 cut(s) 13, 556
Csp6I GTAC 4 cut(s) 331, 841, 883, 979
CspAI ACCGGT 1 cut(s) 496
CviAII CATG 1 cut(s) 1284
CviQI GTAC 4 cut(s) 331, 841, 883, 979
DdeI CTNAG 4 cut(s) 729, 808, 912, 956
DraIII CACNNNGTG 1 cut(s) 281
EaeI YGGCCR 1 cut(s) 504
EciI GGCGGA 1 cut(s) 330
Ecl136II GAGCTC 2 cut(s) 295, 807
Eco130I CCWWGG 2 cut(s) 95, 666
Eco24I GRGCYC 2 cut(s) 297, 809
Eco53kI GAGCTC 2 cut(s) 295, 807
Eco88I CYCGRG 2 cut(s) 5, 158
Eco91I GGTNACC 1 cut(s) 200
EcoICRI GAGCTC 2 cut(s) 295, 807
EcoO65I GGTNACC 1 cut(s) 200
EcoT14I CCWWGG 2 cut(s) 95, 666
EcoT38I GRGCYC 2 cut(s) 297, 809
ErhI CCWWGG 2 cut(s) 95, 666
FaeI CATG 1 cut(s) 1287
FaqI GGGAC 1 cut(s) 892
FatI CATG 1 cut(s) 1283
FauI CCCGC 1 cut(s) 464
FbaI TGATCA 1 cut(s) 1245
FblI GTMKAC 1 cut(s) 420
Fnu4HI GCNGC 4 cut(s) 853, 1207, 1240, 1281
FokI GGATG 1 cut(s) 1141
FriOI GRGCYC 2 cut(s) 297, 809
Fsp4HI GCNGC 4 cut(s) 853, 1207, 1240, 1281
FspBI CTAG 2 cut(s) 96, 963
GlaI GCGC 2 cut(s) 1217, 1228
GluI GCNGC 4 cut(s) 853, 1207, 1240, 1281
HaeII RGCGCY 1 cut(s) 1230
HaeIII GGCC 1 cut(s) 506
HapII CCGG 7 cut(s) 33, 378, 455, 468, 497, 704, 1010
HgaI GACGC 2 cut(s) 13, 556
HhaI GCGC 2 cut(s) 1218, 1229
Hin1I GRCGYC 1 cut(s) 548
Hin1II CATG 1 cut(s) 1287
Hin6I GCGC 2 cut(s) 1216, 1227
HinP1I GCGC 2 cut(s) 1216, 1227
HincII GTYRAC 2 cut(s) 421, 1102
HindII GTYRAC 2 cut(s) 421, 1102
HindIII AAGCTT 2 cut(s) 563, 740
HinfI GANTC 5 cut(s) 222, 233, 337, 346, 1115
HpaI GTTAAC 1 cut(s) 1102
HpaII CCGG 7 cut(s) 33, 378, 455, 468, 497, 704, 1010
HphI GGTGA 6 cut(s) 22, 212, 457, 904, 1033, 1072
Hpy166II GTNNAC 5 cut(s) 40, 421, 465, 895, 1102
Hpy188I TCNGA 9 cut(s) 91, 177, 186, 193, 221, 238, 270, 442, 512
Hpy8I GTNNAC 5 cut(s) 40, 421, 465, 895, 1102
HpyCH4III ACNGT 3 cut(s) 134, 200, 418
HpyCH4IV ACGT 5 cut(s) 12, 45, 78, 280, 881
HpyCH4V TGCA 3 cut(s) 116, 1272, 1283
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 512, 677, 1215, 1224, 1269
HpyF3I CTNAG 4 cut(s) 729, 808, 912, 956
HpySE526I ACGT 5 cut(s) 12, 45, 78, 280, 881
Hsp92I GRCGYC 1 cut(s) 548
Hsp92II CATG 1 cut(s) 1287
HspAI GCGC 2 cut(s) 1216, 1227
KpnI GGTACC 1 cut(s) 982
KroI GCCGGC 1 cut(s) 703
KroNI GCCGGC 1 cut(s) 705
Ksp22I TGATCA 1 cut(s) 1245
KspAI GTTAAC 1 cut(s) 1102
LmnI GCTCC 6 cut(s) 91, 163, 392, 512, 1041, 1071
Lsp1109I GCAGC 4 cut(s) 864, 1193, 1251, 1267
MaeI CTAG 2 cut(s) 96, 963
MaeII ACGT 5 cut(s) 12, 45, 78, 280, 881
MaeIII GTNAC 6 cut(s) 200, 412, 601, 779, 1048, 1187
MboII GAAGA 4 cut(s) 19, 96, 883, 1030
MhlI GDGCHC 3 cut(s) 297, 809, 1038
MlsI TGGCCA 1 cut(s) 506
MluCI AATT 6 cut(s) 140, 542, 594, 612, 887, 1255
MluNI TGGCCA 1 cut(s) 506
MmeI TCCRAC 3 cut(s) 216, 283, 633
Mox20I TGGCCA 1 cut(s) 506
MreI CGCCGGCG 1 cut(s) 703
MroNI GCCGGC 1 cut(s) 703
MscI TGGCCA 1 cut(s) 506
MseI TTAA 4 cut(s) 630, 798, 969, 1101
MslI CAYNNNNRTG 1 cut(s) 1031
Msp20I TGGCCA 1 cut(s) 506
MspI CCGG 7 cut(s) 33, 378, 455, 468, 497, 704, 1010
MspR9I CCNGG 1 cut(s) 1011
Mva1269I GAATGC 1 cut(s) 1054
MvnI CGCG 2 cut(s) 24, 1216
MwoI GCNNNNNNNGC 6 cut(s) 80, 512, 677, 1215, 1224, 1269
NaeI GCCGGC 1 cut(s) 705
NciI CCSGG 1 cut(s) 1011
NgoMIV GCCGGC 1 cut(s) 703
NlaIII CATG 1 cut(s) 1287
NlaIV GGNNCC 5 cut(s) 93, 165, 447, 495, 980
NmeAIII GCCGAG 1 cut(s) 1153
NmuCI GTSAC 5 cut(s) 200, 412, 601, 779, 1048
NspV TTCGAA 1 cut(s) 540
OliI CACNNNNGTG 1 cut(s) 1031
PaeR7I CTCGAG 1 cut(s) 5
PcsI WCGNNNNNNNCGW 8 cut(s) 12, 21, 156, 274, 318, 341, 417, 446
PctI GAATGC 1 cut(s) 1054
PdiI GCCGGC 1 cut(s) 705
PfeI GAWTC 5 cut(s) 222, 233, 337, 346, 1115
PinAI ACCGGT 1 cut(s) 496
PkrI GCNGC 4 cut(s) 854, 1208, 1241, 1282
Ple19I CGATCG 1 cut(s) 22
Psp124BI GAGCTC 2 cut(s) 297, 809
PspEI GGTNACC 1 cut(s) 200
PspN4I GGNNCC 5 cut(s) 93, 165, 447, 495, 980
PvuI CGATCG 1 cut(s) 22
RsaI GTAC 4 cut(s) 332, 842, 884, 980
RsaNI GTAC 4 cut(s) 331, 841, 883, 979
RseI CAYNNNNRTG 1 cut(s) 1031
SacI GAGCTC 2 cut(s) 297, 809
SalI GTCGAC 1 cut(s) 419
SaqAI TTAA 4 cut(s) 630, 798, 969, 1101
SatI GCNGC 4 cut(s) 853, 1207, 1240, 1281
ScrFI CCNGG 1 cut(s) 1011
SduI GDGCHC 3 cut(s) 297, 809, 1038
Sfr274I CTCGAG 1 cut(s) 5
SfuI TTCGAA 1 cut(s) 540
SgrAI CRCCGGYG 3 cut(s) 32, 467, 703
SlaI CTCGAG 1 cut(s) 5
SmiMI CAYNNNNRTG 1 cut(s) 1031
SmlI CTYRAG 2 cut(s) 5, 290
SmoI CTYRAG 2 cut(s) 5, 290
Sse9I AATT 6 cut(s) 140, 542, 594, 612, 887, 1255
SsiI CCGC 2 cut(s) 341, 471
SspMI CTAG 2 cut(s) 96, 963
SstI GAGCTC 2 cut(s) 297, 809
StyD4I CCNGG 1 cut(s) 1009
StyI CCWWGG 2 cut(s) 95, 666
TaaI ACNGT 3 cut(s) 134, 200, 418
TaiI ACGT 5 cut(s) 15, 48, 81, 283, 884
TaqI TCGA 9 cut(s) 6, 18, 48, 107, 225, 335, 420, 540, 803
TaqII GACCGA 2 cut(s) 219, 620
TasI AATT 6 cut(s) 140, 542, 594, 612, 887, 1255
TatI WGTACW 1 cut(s) 840
TfiI GAWTC 5 cut(s) 222, 233, 337, 346, 1115
Tru1I TTAA 4 cut(s) 630, 798, 969, 1101
Tru9I TTAA 4 cut(s) 630, 798, 969, 1101
TseFI GTSAC 5 cut(s) 200, 412, 601, 779, 1048
TseI GCWGC 4 cut(s) 852, 1206, 1239, 1280
Tsp45I GTSAC 5 cut(s) 200, 412, 601, 779, 1048
TspDTI ATGAA 3 cut(s) 44, 606, 1256
TspGWI ACGGA 1 cut(s) 471
XapI RAATTY 1 cut(s) 612
XhoI CTCGAG 1 cut(s) 5
XmaJI CCTAGG 1 cut(s) 95
XmiI GTMKAC 1 cut(s) 420
XspI CTAG 2 cut(s) 96, 963
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.