Rmu_co8367915.1_g000001

amidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8367915.1
OG0017136
Physical Location & Seq
Forward (+)
1 .. 944
944 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8367915.1_g000001.1.cds

Sequence Viewer

Length: 944 bp
tatacatcacgacgaacaatctgacaaccggacgaggaaatggagcttacagagagcttttcgtgacggtcgacggccagttggtgggatcggaggtgccgtttccggtggtgttcaccggcgggatcaatccgttgttttgggaaccggtggtggccatcggagctttcaaccttccttcttatgatttcgaattgacgccgtttctggggaagcttctggataagaaggctcatagctttggaattggagtgaccgatggaatttcatattggctcgttaatgccaacttacacatttggttggatcataaatccaaggctgtgaaagctaaatcaaactcgtttccttcgccggcggttgaagtgaagcgtaatgctcagttcaaaaagcttgacgggtcgttcaggatcaaggcaaggaggaggagtgactttgtggggtgggttaagtcgagctcagggaactacactaccacattctcgcagaagtacaagttcagcagctcggttagttttgggaagaacgggacgtacaaattggtgaaccagaggatcaaggctgagaaggaagtgagggttgtgagtgagattggtgtggtggttcttagaattagggttaagggaaggtaccctattgatgttattacagctactctaccaggtaagaagaaagacacctatgtgctcctcaccaatgtcacgcattcggtgaaggagaggagctggcacgggggatatttgagccgtgttaacaacattcaagattcaagagggtggatggaggtcagggatcattctgttctttctggaacagcagacactcagcagagcttcggttacaaagataactttggctgctactcgcgcactattggcgctgttgatggcagcttgatcagcgataattccagctttcattgcatatctgctgcacgattctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

313

Amino Acids

34.8

Weight (kDa)

9.81

Isoelectric Point (pI)

30.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05480
fragaria_vesca FvH4_7g24990
prunus_persica Prupe.2G255000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0370891
rosa_laevigata RLG00000027001
rosa_multiflora Rmu_co8367915.1_g000001
rosa_roxburghii Rroxscaffold_4G00286150
rosa_rugosa Rorug01G0360200
rosa_samantha Rh1AG370000 Rh1BG333600 Rh1CG346900 Rh1DG364800
rosa_wichuraiana Rw1G032560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 629
AccB1I GGYRCC 2 cut(s) 96, 629
AccB7I CCANNNNNTGG 1 cut(s) 84
AccI GTMKAC 1 cut(s) 71
AccII CGCG 1 cut(s) 867
AciI CCGC 2 cut(s) 122, 358
AclWI GGATC 6 cut(s) 96, 133, 314, 418, 562, 800
AcoI YGGCCR 2 cut(s) 75, 155
AcsI RAATTY 1 cut(s) 263
AcyI GRCGYC 1 cut(s) 199
AfaI GTAC 3 cut(s) 493, 535, 631
AfiI CCNNNNNNNGG 2 cut(s) 84, 208
AgeI ACCGGT 1 cut(s) 147
AgsI TTSAA 5 cut(s) 171, 364, 387, 763, 770
AjnI CCWGG 1 cut(s) 660
AjuI GAANNNNNNNTTGG 2 cut(s) 255, 287
AleI CACNNNNGTG 1 cut(s) 682
Alw21I GWGCWC 2 cut(s) 460, 689
AlwI GGATC 6 cut(s) 96, 133, 314, 418, 562, 800
AoxI GGCC 2 cut(s) 75, 155
ApeKI GCWGC 4 cut(s) 503, 857, 890, 931
ApoI RAATTY 1 cut(s) 263
AsiGI ACCGGT 1 cut(s) 147
Asp718I GGTACC 1 cut(s) 629
AspLEI GCGC 2 cut(s) 869, 880
AsuHPI GGTGA 4 cut(s) 108, 555, 684, 723
AsuII TTCGAA 1 cut(s) 191
BalI TGGCCA 1 cut(s) 157
BanI GGYRCC 2 cut(s) 96, 629
BanII GRGCYC 1 cut(s) 460
Bbv12I GWGCWC 2 cut(s) 460, 689
BbvI GCAGC 4 cut(s) 515, 844, 902, 918
BccI CCATC 4 cut(s) 166, 253, 774, 880
BceAI ACGGC 4 cut(s) 84, 90, 186, 731
BciT130I CCWGG 1 cut(s) 662
BclI TGATCA 1 cut(s) 896
BfoI RGCGCY 1 cut(s) 881
BisI GCNGC 4 cut(s) 504, 858, 891, 932
BlsI GCNGC 4 cut(s) 505, 859, 892, 933
Bme1390I CCNGG 1 cut(s) 662
BmiI GGNNCC 3 cut(s) 98, 146, 631
BmrFI CCNGG 1 cut(s) 662
Bpu10I CCTNAGC 1 cut(s) 459
Bpu14I TTCGAA 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 199
BsaJI CCNNGG 1 cut(s) 317
BsaWI WCCGGW 3 cut(s) 28, 105, 147
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 414, 444
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 208
Bse118I RCCGGY 3 cut(s) 118, 147, 354
Bse1I ACTGG 1 cut(s) 78
Bse3DI GCAATG 1 cut(s) 918
BseBI CCWGG 1 cut(s) 662
BseDI CCNNGG 1 cut(s) 317
BseGI GGATG 1 cut(s) 785
BseLI CCNNNNNNNGG 2 cut(s) 84, 208
BseMI GCAATG 1 cut(s) 918
BseMII CTCAG 4 cut(s) 394, 473, 554, 838
BseNI ACTGG 1 cut(s) 78
BseRI GAGGAG 4 cut(s) 438, 441, 679, 735
BseXI GCAGC 4 cut(s) 515, 844, 902, 918
BsgI GTGCAG 1 cut(s) 917
Bsh1236I CGCG 1 cut(s) 867
Bsh1285I CGRYCG 1 cut(s) 71
BshFI GGCC 2 cut(s) 77, 157
BshNI GGYRCC 2 cut(s) 96, 629
BshTI ACCGGT 1 cut(s) 147
BsiEI CGRYCG 1 cut(s) 71
BsiHKAI GWGCWC 2 cut(s) 460, 689
BsiSI CCGG 5 cut(s) 29, 106, 119, 148, 355
BslFI GGGAC 1 cut(s) 543
BslI CCNNNNNNNGG 2 cut(s) 84, 208
BsmFI GGGAC 1 cut(s) 543
BsmI GAATGC 1 cut(s) 705
BsnI GGCC 2 cut(s) 77, 157
Bsp119I TTCGAA 1 cut(s) 191
Bsp1286I GDGCHC 2 cut(s) 460, 689
Bsp143I GATC 7 cut(s) 88, 125, 306, 410, 554, 792, 896
BspACI CCGC 2 cut(s) 122, 358
BspANI GGCC 2 cut(s) 77, 157
BspCNI CTCAG 4 cut(s) 393, 472, 555, 837
BspFNI CGCG 1 cut(s) 867
BspLI GGNNCC 3 cut(s) 98, 146, 631
BspPI GGATC 6 cut(s) 96, 133, 314, 418, 562, 800
BspT104I TTCGAA 1 cut(s) 191
BspT107I GGYRCC 2 cut(s) 96, 629
BsrDI GCAATG 1 cut(s) 918
BsrFI RCCGGY 3 cut(s) 118, 147, 354
BsrI ACTGG 1 cut(s) 78
BssAI RCCGGY 3 cut(s) 118, 147, 354
BssECI CCNNGG 1 cut(s) 317
BssMI GATC 7 cut(s) 88, 125, 306, 410, 554, 792, 896
BssNI GRCGYC 1 cut(s) 199
BssT1I CCWWGG 1 cut(s) 317
Bst2UI CCWGG 1 cut(s) 662
Bst4CI ACNGT 1 cut(s) 69
BstACI GRCGYC 1 cut(s) 199
BstBI TTCGAA 1 cut(s) 191
BstC8I GCNNGC 2 cut(s) 356, 727
BstDEI CTNAG 5 cut(s) 380, 459, 563, 607, 824
BstF5I GGATG 1 cut(s) 785
BstFNI CGCG 1 cut(s) 867
BstH2I RGCGCY 1 cut(s) 881
BstHHI GCGC 2 cut(s) 869, 880
BstKTI GATC 7 cut(s) 91, 128, 309, 413, 557, 795, 899
BstMBI GATC 7 cut(s) 88, 125, 306, 410, 554, 792, 896
BstMCI CGRYCG 1 cut(s) 71
BstMWI GCNNNNNNNGC 6 cut(s) 163, 328, 866, 875, 899, 920
BstNI CCWGG 1 cut(s) 662
BstSCI CCNGG 1 cut(s) 660
BstUI CGCG 1 cut(s) 867
BstV1I GCAGC 4 cut(s) 515, 844, 902, 918
BsuRI GGCC 2 cut(s) 77, 157
BtsCI GGATG 1 cut(s) 785
Cac8I GCNNGC 2 cut(s) 356, 727
CfoI GCGC 2 cut(s) 869, 880
Cfr10I RCCGGY 3 cut(s) 118, 147, 354
CseI GACGC 1 cut(s) 207
CsiI ACCWGGT 1 cut(s) 660
Csp6I GTAC 3 cut(s) 492, 534, 630
CspAI ACCGGT 1 cut(s) 147
CviQI GTAC 3 cut(s) 492, 534, 630
DdeI CTNAG 5 cut(s) 380, 459, 563, 607, 824
DpnI GATC 7 cut(s) 90, 127, 308, 412, 556, 794, 898
DpnII GATC 7 cut(s) 88, 125, 306, 410, 554, 792, 896
EaeI YGGCCR 2 cut(s) 75, 155
Ecl136II GAGCTC 1 cut(s) 458
Eco130I CCWWGG 1 cut(s) 317
Eco24I GRGCYC 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 458
EcoICRI GAGCTC 1 cut(s) 458
EcoRII CCWGG 1 cut(s) 660
EcoT14I CCWWGG 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 317
FaiI YATR 7 cut(s) 3, 185, 236, 270, 311, 683, 925
FaqI GGGAC 1 cut(s) 543
FauI CCCGC 1 cut(s) 115
FbaI TGATCA 1 cut(s) 896
FblI GTMKAC 1 cut(s) 71
Fnu4HI GCNGC 4 cut(s) 504, 858, 891, 932
FokI GGATG 1 cut(s) 792
FriOI GRGCYC 1 cut(s) 460
Fsp4HI GCNGC 4 cut(s) 504, 858, 891, 932
GlaI GCGC 2 cut(s) 868, 879
GluI GCNGC 4 cut(s) 504, 858, 891, 932
HaeII RGCGCY 1 cut(s) 881
HaeIII GGCC 2 cut(s) 77, 157
HapII CCGG 5 cut(s) 29, 106, 119, 148, 355
HgaI GACGC 1 cut(s) 207
HhaI GCGC 2 cut(s) 869, 880
Hin1I GRCGYC 1 cut(s) 199
Hin6I GCGC 2 cut(s) 867, 878
HinP1I GCGC 2 cut(s) 867, 878
HincII GTYRAC 2 cut(s) 72, 753
HindII GTYRAC 2 cut(s) 72, 753
HindIII AAGCTT 2 cut(s) 214, 391
HinfI GANTC 2 cut(s) 766, 938
HpaI GTTAAC 1 cut(s) 753
HpaII CCGG 5 cut(s) 29, 106, 119, 148, 355
HphI GGTGA 4 cut(s) 108, 555, 684, 723
Hpy166II GTNNAC 4 cut(s) 72, 116, 546, 753
Hpy188I TCNGA 4 cut(s) 23, 93, 163, 943
Hpy188III TCNNGA 7 cut(s) 9, 63, 220, 408, 763, 770, 809
Hpy8I GTNNAC 4 cut(s) 72, 116, 546, 753
Hpy99I CGWCG 2 cut(s) 15, 76
HpyAV CCTTC 7 cut(s) 184, 188, 222, 359, 561, 620, 708
HpyCH4III ACNGT 1 cut(s) 69
HpyCH4IV ACGT 1 cut(s) 532
HpyCH4V TGCA 2 cut(s) 923, 934
HpyF10VI GCNNNNNNNGC 6 cut(s) 163, 328, 866, 875, 899, 920
HpyF3I CTNAG 5 cut(s) 380, 459, 563, 607, 824
HpySE526I ACGT 1 cut(s) 532
Hsp92I GRCGYC 1 cut(s) 199
HspAI GCGC 2 cut(s) 867, 878
KpnI GGTACC 1 cut(s) 633
KroI GCCGGC 1 cut(s) 354
KroNI GCCGGC 1 cut(s) 356
Ksp22I TGATCA 1 cut(s) 896
KspAI GTTAAC 1 cut(s) 753
Kzo9I GATC 7 cut(s) 88, 125, 306, 410, 554, 792, 896
LmnI GCTCC 4 cut(s) 43, 163, 692, 722
Lsp1109I GCAGC 4 cut(s) 515, 844, 902, 918
MabI ACCWGGT 1 cut(s) 660
MaeII ACGT 1 cut(s) 532
MaeIII GTNAC 5 cut(s) 63, 252, 430, 699, 838
MalI GATC 7 cut(s) 90, 127, 308, 412, 556, 794, 898
MboI GATC 7 cut(s) 88, 125, 306, 410, 554, 792, 896
MboII GAAGA 2 cut(s) 534, 681
MhlI GDGCHC 2 cut(s) 460, 689
MlsI TGGCCA 1 cut(s) 157
MluCI AATT 6 cut(s) 193, 245, 263, 538, 611, 906
MluNI TGGCCA 1 cut(s) 157
MmeI TCCRAC 1 cut(s) 284
Mox20I TGGCCA 1 cut(s) 157
MreI CGCCGGCG 1 cut(s) 354
MroNI GCCGGC 1 cut(s) 354
MscI TGGCCA 1 cut(s) 157
MseI TTAA 4 cut(s) 281, 449, 620, 752
MslI CAYNNNNRTG 1 cut(s) 682
Msp20I TGGCCA 1 cut(s) 157
MspI CCGG 5 cut(s) 29, 106, 119, 148, 355
MspR9I CCNGG 1 cut(s) 662
Mva1269I GAATGC 1 cut(s) 705
MvaI CCWGG 1 cut(s) 662
MvnI CGCG 1 cut(s) 867
MwoI GCNNNNNNNGC 6 cut(s) 163, 328, 866, 875, 899, 920
NaeI GCCGGC 1 cut(s) 356
NdeII GATC 7 cut(s) 88, 125, 306, 410, 554, 792, 896
NgoMIV GCCGGC 1 cut(s) 354
NlaIV GGNNCC 3 cut(s) 98, 146, 631
NmuCI GTSAC 4 cut(s) 63, 252, 430, 699
NspV TTCGAA 1 cut(s) 191
OliI CACNNNNGTG 1 cut(s) 682
PcsI WCGNNNNNNNCGW 2 cut(s) 68, 97
PctI GAATGC 1 cut(s) 705
PdiI GCCGGC 1 cut(s) 356
PfeI GAWTC 2 cut(s) 766, 938
PflMI CCANNNNNTGG 1 cut(s) 84
PinAI ACCGGT 1 cut(s) 147
PkrI GCNGC 4 cut(s) 505, 859, 892, 933
Psp124BI GAGCTC 1 cut(s) 460
Psp6I CCWGG 1 cut(s) 660
PspGI CCWGG 1 cut(s) 660
PspN4I GGNNCC 3 cut(s) 98, 146, 631
PsrI GAACNNNNNNTAC 2 cut(s) 517, 549
RsaI GTAC 3 cut(s) 493, 535, 631
RsaNI GTAC 3 cut(s) 492, 534, 630
RseI CAYNNNNRTG 1 cut(s) 682
SacI GAGCTC 1 cut(s) 460
SalI GTCGAC 1 cut(s) 70
SaqAI TTAA 4 cut(s) 281, 449, 620, 752
SatI GCNGC 4 cut(s) 504, 858, 891, 932
Sau3AI GATC 7 cut(s) 88, 125, 306, 410, 554, 792, 896
ScrFI CCNGG 1 cut(s) 662
SduI GDGCHC 2 cut(s) 460, 689
SexAI ACCWGGT 1 cut(s) 660
SfuI TTCGAA 1 cut(s) 191
SgrAI CRCCGGYG 2 cut(s) 118, 354
SmiMI CAYNNNNRTG 1 cut(s) 682
Sse9I AATT 6 cut(s) 193, 245, 263, 538, 611, 906
SsiI CCGC 2 cut(s) 122, 358
SstI GAGCTC 1 cut(s) 460
StyD4I CCNGG 1 cut(s) 660
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 1 cut(s) 69
TaiI ACGT 1 cut(s) 535
TaqI TCGA 3 cut(s) 71, 191, 454
TaqII GACCGA 1 cut(s) 271
TasI AATT 6 cut(s) 193, 245, 263, 538, 611, 906
TatI WGTACW 1 cut(s) 491
TfiI GAWTC 2 cut(s) 766, 938
Tru1I TTAA 4 cut(s) 281, 449, 620, 752
Tru9I TTAA 4 cut(s) 281, 449, 620, 752
TseFI GTSAC 4 cut(s) 63, 252, 430, 699
TseI GCWGC 4 cut(s) 503, 857, 890, 931
Tsp45I GTSAC 4 cut(s) 63, 252, 430, 699
TspDTI ATGAA 2 cut(s) 257, 907
TspGWI ACGGA 1 cut(s) 122
Van91I CCANNNNNTGG 1 cut(s) 84
XapI RAATTY 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.