Rroxscaffold_4G00286150

amidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0017136
Physical Location & Seq
Reverse (-)
7304461 .. 7306232
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00286150.1

Sequence Viewer

Length: 1722 bp
ATGTACGGCCTCCTCTCTCTCCTCCTCCTCCTCCTCCGCTTCTTCGCCGCCGCAGTCTCCGCCTCCTCCACCTCCTTCCCGGACCGTCGGCCGTCCTCTGCCGTCCGATCTCCTCCCCCGTCCTGCTACCGCCGCGTCCTCACCCACTCCTTCGCCAACACCATCAACGGCCCTCCCTTCTCCGCCCCATACTCCCCTCCGCCGCCCTCCGAATCCTCCGTTTGCTCCGCCCCATGGTCCCACGTCGCCCTCGAGTTTAGCGCCAAATGCAAAGGCGAGCAGTACGACCGCATCGCCGGGCTTTGGCTCGGCGGCGTTGAGCTCCTCCGTACCAGCACCGCCGAGCCGACCAAGAAGGGAATCTTCTGGAAGGTTCGCAAGGACATCACTAAATACTCCTCCGTCCTAGCTCGCTGCGATCTCAACCTCACCATGATGCTCGAGAACGTCGTCGATCGCGTCTTCACCGGCGTTTACCACGTCGAGGTTCAATTCCTCTATTACAAGGCAAACGTAGCCGTTTCGGAGCCTAGGGTTTCTTCGATCCTTGCAGATCAAACTTTCAACGGTCAAAAATTGGGGATCGTTGTTCCTGAGGAGCCTGCCGATTTGATCGTTCCGATCTCCGACGACGGCGACAGAGGCTTTTGGTTCAGAATCGACAGCGAATCGGATCTCCCTTCCAAGCAAATCCAACTCCCTCGGAACACACGACGTGTCGTTCTTGAGCTCTACGTTTCGTTTCACGGCAACGACGAGTTCTGGTACTCGAATCCGCCGAATCTATACATCACGACGAACAATCTGACAACCGGACGAGGAAATGGAGCTTACAGAGAGCTTTTCGTGACGGTTGACGGCGAGCTGGTGGGATCGGAGGTGCCGTTTCCGGTGGTCTTCACCGGCGGGATCAATCCGTTGTTTTGGGAACCGGTGGTAGCGATCGGAGCTTTCAACCTTCCTTCTTACGATTTCGAATTGACGCCGTTTCTGGGGAAGCTTCTGGATAAGAAGGCTCATAGCTTTGGAATTGGAGTGACCGATGGAATTTCATATTGGCTTGTTAATGCCAACTTACACATTTGGTTGGATCATAAATCCAAGGCTGTGAAAGCTAAATCAAACTCGTTTCCTTCGCCGGCGATTGAAGTGAAGCGTAATGCTCAGTTCAAAAAGCTTGACGGGTCGTTCAGGATCAAGGCAAGGAGGAGGAGTGACTTTGTGGGGTGGGTTAAGTCGAGCTCAGGGAACTACACTACCACATTCTCGCAGAAGTACAAGTTCAGCAGCTCGGTTAGTTTTGGGAAGAACGGGACGTACAAATTGGTGAACCAGAAGGTCAAGGCTGAGAAGGAAGTGAGGGTTGTGAGTGAGATTGGTGTGGTGGTTCTTAGAACCAGGGTTAAGGGAAGGTACCCTATTGATGTTTTTACAGCTACTCTACCAGGGAAGAAGAAAGACACCTATGTGCTCCTCACCAATGTCACGCATTCGGTGAAGGAGAGGAGCTGGCACGGGGGATATTTGAGCCGTCTTAACAACATTCAAGATTCAAGAGGGTGGATGGAGGTCAGGGATCATTCTGTTCTTTCTGGAACAGCAGACACTCGGCAGAGTTTCGGTTACAAAGATAACTTTGGCTGCTACTCGCGCACTATTGGCGCTGTTGATGGCAGCTTGATCAGGGATAATTCCAGCCTTCATTGCATATCTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

573

Amino Acids

63.85

Weight (kDa)

9.45

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNGaseA PF12222 40 - 375 1.7e-91 Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
PNGase_A_C PF25156 396 - 453 1.1e-06 PNGase_A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05480
fragaria_vesca FvH4_7g24990
prunus_persica Prupe.2G255000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0370891
rosa_laevigata RLG00000027001
rosa_multiflora Rmu_co8367915.1_g000001
rosa_roxburghii Rroxscaffold_4G00286150
rosa_rugosa Rorug01G0360200
rosa_samantha Rh1AG370000 Rh1BG333600 Rh1CG346900 Rh1DG364800
rosa_wichuraiana Rw1G032560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1413
AccB1I GGYRCC 2 cut(s) 880, 1413
AccII CGCG 3 cut(s) 135, 459, 1651
AclWI GGATC 8 cut(s) 538, 590, 681, 880, 917, 1098, 1202, 1584
AcoI YGGCCR 1 cut(s) 89
AcsI RAATTY 1 cut(s) 1047
AcyI GRCGYC 1 cut(s) 983
AdeI CACNNNGTG 1 cut(s) 716
AfaI GTAC 7 cut(s) 5, 284, 331, 767, 1277, 1319, 1415
AfiI CCNNNNNNNGG 4 cut(s) 234, 303, 484, 992
AflIII ACRYGT 1 cut(s) 715
AgeI ACCGGT 1 cut(s) 931
AgsI TTSAA 7 cut(s) 491, 565, 955, 1148, 1171, 1547, 1554
AjiI CACGTC 3 cut(s) 244, 481, 716
AjnI CCWGG 2 cut(s) 1397, 1444
AjuI GAANNNNNNNTTGG 2 cut(s) 1039, 1071
AleI CACNNNNGTG 1 cut(s) 1466
Alw21I GWGCWC 4 cut(s) 324, 732, 1244, 1473
Alw26I GTCTC 1 cut(s) 61
AlwI GGATC 8 cut(s) 538, 590, 681, 880, 917, 1098, 1202, 1584
Ama87I CYCGRG 2 cut(s) 251, 440
AoxI GGCC 3 cut(s) 7, 89, 169
ApeKI GCWGC 5 cut(s) 414, 1287, 1641, 1674, 1715
ApoI RAATTY 1 cut(s) 1047
AsiGI ACCGGT 1 cut(s) 931
Asp718I GGTACC 1 cut(s) 1413
AspA2I CCTAGG 1 cut(s) 530
AspLEI GCGC 3 cut(s) 263, 1653, 1664
AspS9I GGNCC 3 cut(s) 82, 170, 237
AsuC2I CCSGG 2 cut(s) 80, 298
AsuHPI GGTGA 7 cut(s) 133, 421, 457, 892, 1339, 1468, 1507
AsuII TTCGAA 1 cut(s) 975
AvaI CYCGRG 2 cut(s) 251, 440
AvaII GGWCC 2 cut(s) 82, 237
AvrII CCTAGG 1 cut(s) 530
AxyI CCTNAGG 1 cut(s) 594
BanI GGYRCC 2 cut(s) 880, 1413
BanII GRGCYC 3 cut(s) 324, 732, 1244
BbsI GAAGAC 2 cut(s) 454, 889
Bbv12I GWGCWC 4 cut(s) 324, 732, 1244, 1473
BbvI GCAGC 5 cut(s) 401, 1299, 1628, 1686, 1702
BccI CCATC 4 cut(s) 170, 1037, 1558, 1664
BciT130I CCWGG 2 cut(s) 1399, 1446
BclI TGATCA 1 cut(s) 1680
BcnI CCSGG 2 cut(s) 80, 298
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 2 cut(s) 407, 531
BfoI RGCGCY 2 cut(s) 264, 1665
BlnI CCTAGG 1 cut(s) 530
Bme1390I CCNGG 4 cut(s) 80, 298, 1399, 1446
Bme18I GGWCC 2 cut(s) 82, 237
BmeT110I CYCGRG 2 cut(s) 251, 440
BmgBI CACGTC 3 cut(s) 244, 481, 716
BmgT120I GGNCC 3 cut(s) 82, 170, 237
BmiI GGNNCC 6 cut(s) 239, 528, 600, 882, 930, 1415
BmrFI CCNGG 4 cut(s) 80, 298, 1399, 1446
BmsI GCATC 2 cut(s) 300, 426
BpiI GAAGAC 2 cut(s) 454, 889
Bpu10I CCTNAGC 1 cut(s) 1243
Bpu14I TTCGAA 1 cut(s) 975
BpuEI CTTGAG 1 cut(s) 746
BpuMI CCSGG 2 cut(s) 80, 298
BsaHI GRCGYC 1 cut(s) 983
BsaJI CCNNGG 6 cut(s) 233, 530, 701, 1101, 1398, 1445
BsaWI WCCGGW 3 cut(s) 812, 889, 931
BsaXI ACNNNNNCTCC 4 cut(s) 869, 899, 1198, 1228
Bsc4I CCNNNNNNNGG 4 cut(s) 234, 303, 484, 992
Bse118I RCCGGY 4 cut(s) 467, 902, 931, 1138
Bse21I CCTNAGG 1 cut(s) 594
Bse3DI GCAATG 1 cut(s) 1702
BseBI CCWGG 2 cut(s) 1399, 1446
BseDI CCNNGG 6 cut(s) 233, 530, 701, 1101, 1398, 1445
BseGI GGATG 1 cut(s) 1569
BseLI CCNNNNNNNGG 4 cut(s) 234, 303, 484, 992
BseMI GCAATG 1 cut(s) 1702
BseMII CTCAG 4 cut(s) 585, 1178, 1257, 1338
BseX3I CGGCCG 1 cut(s) 89
BseXI GCAGC 5 cut(s) 401, 1299, 1628, 1686, 1702
Bsh1236I CGCG 3 cut(s) 135, 459, 1651
Bsh1285I CGRYCG 4 cut(s) 92, 289, 457, 945
BshFI GGCC 3 cut(s) 9, 91, 171
BshNI GGYRCC 2 cut(s) 880, 1413
BshTI ACCGGT 1 cut(s) 931
BsiEI CGRYCG 4 cut(s) 92, 289, 457, 945
BsiHKAI GWGCWC 4 cut(s) 324, 732, 1244, 1473
BsiHKCI CYCGRG 2 cut(s) 251, 440
BsiSI CCGG 8 cut(s) 80, 297, 468, 813, 890, 903, 932, 1139
BslFI GGGAC 2 cut(s) 223, 1327
BslI CCNNNNNNNGG 4 cut(s) 234, 303, 484, 992
BsmAI GTCTC 1 cut(s) 61
BsmFI GGGAC 2 cut(s) 223, 1327
BsmI GAATGC 1 cut(s) 1489
BsnI GGCC 3 cut(s) 9, 91, 171
BsoBI CYCGRG 2 cut(s) 251, 440
Bsp119I TTCGAA 1 cut(s) 975
Bsp1286I GDGCHC 4 cut(s) 324, 732, 1244, 1473
Bsp19I CCATGG 1 cut(s) 233
BspANI GGCC 3 cut(s) 9, 91, 171
BspCNI CTCAG 4 cut(s) 586, 1177, 1256, 1339
BspFNI CGCG 3 cut(s) 135, 459, 1651
BspLI GGNNCC 6 cut(s) 239, 528, 600, 882, 930, 1415
BspPI GGATC 8 cut(s) 538, 590, 681, 880, 917, 1098, 1202, 1584
BspT104I TTCGAA 1 cut(s) 975
BspT107I GGYRCC 2 cut(s) 880, 1413
BsrDI GCAATG 1 cut(s) 1702
BsrFI RCCGGY 4 cut(s) 467, 902, 931, 1138
BssAI RCCGGY 4 cut(s) 467, 902, 931, 1138
BssECI CCNNGG 6 cut(s) 233, 530, 701, 1101, 1398, 1445
BssNI GRCGYC 1 cut(s) 983
BssT1I CCWWGG 3 cut(s) 233, 530, 1101
Bst2UI CCWGG 2 cut(s) 1399, 1446
Bst4CI ACNGT 3 cut(s) 86, 569, 853
BstACI GRCGYC 1 cut(s) 983
BstBI TTCGAA 1 cut(s) 975
BstC8I GCNNGC 6 cut(s) 278, 412, 603, 863, 1140, 1511
BstDEI CTNAG 5 cut(s) 594, 1164, 1243, 1347, 1391
BstDSI CCRYGG 1 cut(s) 233
BstF5I GGATG 1 cut(s) 1569
BstFNI CGCG 3 cut(s) 135, 459, 1651
BstH2I RGCGCY 2 cut(s) 264, 1665
BstHHI GCGC 3 cut(s) 263, 1653, 1664
BstMAI GTCTC 1 cut(s) 61
BstMCI CGRYCG 4 cut(s) 92, 289, 457, 945
BstNI CCWGG 2 cut(s) 1399, 1446
BstSCI CCNGG 4 cut(s) 78, 296, 1397, 1444
BstUI CGCG 3 cut(s) 135, 459, 1651
BstV1I GCAGC 5 cut(s) 401, 1299, 1628, 1686, 1702
BstV2I GAAGAC 2 cut(s) 454, 889
BstX2I RGATCY 1 cut(s) 673
BstYI RGATCY 1 cut(s) 673
BstZI CGGCCG 1 cut(s) 89
Bsu36I CCTNAGG 1 cut(s) 594
BsuRI GGCC 3 cut(s) 9, 91, 171
BtgI CCRYGG 1 cut(s) 233
BtgZI GCGATG 1 cut(s) 277
BtrI CACGTC 3 cut(s) 244, 481, 716
BtsCI GGATG 1 cut(s) 1569
Cac8I GCNNGC 6 cut(s) 278, 412, 603, 863, 1140, 1511
CfoI GCGC 3 cut(s) 263, 1653, 1664
Cfr10I RCCGGY 4 cut(s) 467, 902, 931, 1138
Cfr13I GGNCC 3 cut(s) 82, 170, 237
CpoI CGGWCCG 1 cut(s) 82
CseI GACGC 3 cut(s) 124, 448, 991
Csp6I GTAC 7 cut(s) 4, 283, 330, 766, 1276, 1318, 1414
CspAI ACCGGT 1 cut(s) 931
CspI CGGWCCG 1 cut(s) 82
CviAII CATG 3 cut(s) 234, 433, 1719
CviQI GTAC 7 cut(s) 4, 283, 330, 766, 1276, 1318, 1414
DdeI CTNAG 5 cut(s) 594, 1164, 1243, 1347, 1391
DraIII CACNNNGTG 1 cut(s) 716
EaeI YGGCCR 1 cut(s) 89
EagI CGGCCG 1 cut(s) 89
EciI GGCGGA 5 cut(s) 49, 172, 189, 217, 765
Ecl136II GAGCTC 3 cut(s) 322, 730, 1242
EclXI CGGCCG 1 cut(s) 89
Eco130I CCWWGG 3 cut(s) 233, 530, 1101
Eco24I GRGCYC 3 cut(s) 324, 732, 1244
Eco47I GGWCC 2 cut(s) 82, 237
Eco52I CGGCCG 1 cut(s) 89
Eco53kI GAGCTC 3 cut(s) 322, 730, 1242
Eco81I CCTNAGG 1 cut(s) 594
Eco88I CYCGRG 2 cut(s) 251, 440
EcoICRI GAGCTC 3 cut(s) 322, 730, 1242
EcoRII CCWGG 2 cut(s) 1397, 1444
EcoT14I CCWWGG 3 cut(s) 233, 530, 1101
EcoT38I GRGCYC 3 cut(s) 324, 732, 1244
ErhI CCWWGG 3 cut(s) 233, 530, 1101
FaeI CATG 3 cut(s) 237, 436, 1722
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 2 cut(s) 223, 1327
FatI CATG 3 cut(s) 233, 432, 1718
FauI CCCGC 1 cut(s) 899
FbaI TGATCA 1 cut(s) 1680
FokI GGATG 1 cut(s) 1576
FriOI GRGCYC 3 cut(s) 324, 732, 1244
FspBI CTAG 2 cut(s) 407, 531
GlaI GCGC 3 cut(s) 262, 1652, 1663
HaeII RGCGCY 2 cut(s) 264, 1665
HaeIII GGCC 3 cut(s) 9, 91, 171
HapII CCGG 8 cut(s) 80, 297, 468, 813, 890, 903, 932, 1139
HgaI GACGC 3 cut(s) 124, 448, 991
HhaI GCGC 3 cut(s) 263, 1653, 1664
Hin1I GRCGYC 1 cut(s) 983
Hin1II CATG 3 cut(s) 237, 436, 1722
Hin6I GCGC 3 cut(s) 261, 1651, 1662
HinP1I GCGC 3 cut(s) 261, 1651, 1662
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 2 cut(s) 998, 1175
HinfI GANTC 7 cut(s) 212, 360, 657, 668, 772, 781, 1550
HpaII CCGG 8 cut(s) 80, 297, 468, 813, 890, 903, 932, 1139
HphI GGTGA 7 cut(s) 133, 421, 457, 892, 1339, 1468, 1507
Hpy166II GTNNAC 3 cut(s) 475, 856, 1330
Hpy8I GTNNAC 3 cut(s) 475, 856, 1330
HpyCH4III ACNGT 3 cut(s) 86, 569, 853
HpyCH4IV ACGT 7 cut(s) 243, 447, 480, 513, 715, 735, 1316
HpyCH4V TGCA 4 cut(s) 270, 551, 1707, 1718
HpyF3I CTNAG 5 cut(s) 594, 1164, 1243, 1347, 1391
HpySE526I ACGT 7 cut(s) 243, 447, 480, 513, 715, 735, 1316
Hsp92I GRCGYC 1 cut(s) 983
Hsp92II CATG 3 cut(s) 237, 436, 1722
HspAI GCGC 3 cut(s) 261, 1651, 1662
KpnI GGTACC 1 cut(s) 1417
KroI GCCGGC 1 cut(s) 1138
KroNI GCCGGC 1 cut(s) 1140
Ksp22I TGATCA 1 cut(s) 1680
LmnI GCTCC 8 cut(s) 230, 327, 526, 598, 827, 947, 1476, 1506
Lsp1109I GCAGC 5 cut(s) 401, 1299, 1628, 1686, 1702
LweI GCATC 2 cut(s) 300, 426
MaeI CTAG 2 cut(s) 407, 531
MaeII ACGT 7 cut(s) 243, 447, 480, 513, 715, 735, 1316
MaeIII GTNAC 5 cut(s) 847, 1036, 1214, 1483, 1622
MboII GAAGA 8 cut(s) 34, 355, 454, 531, 889, 1318, 1462, 1465
MflI RGATCY 1 cut(s) 673
MhlI GDGCHC 4 cut(s) 324, 732, 1244, 1473
MluCI AATT 7 cut(s) 491, 575, 977, 1029, 1047, 1322, 1690
MmeI TCCRAC 3 cut(s) 651, 718, 1068
MreI CGCCGGCG 1 cut(s) 1138
MroNI GCCGGC 1 cut(s) 1138
MseI TTAA 4 cut(s) 1065, 1233, 1404, 1536
MslI CAYNNNNRTG 2 cut(s) 434, 1466
MspI CCGG 8 cut(s) 80, 297, 468, 813, 890, 903, 932, 1139
MspR9I CCNGG 4 cut(s) 80, 298, 1399, 1446
Mva1269I GAATGC 1 cut(s) 1489
MvaI CCWGG 2 cut(s) 1399, 1446
MvnI CGCG 3 cut(s) 135, 459, 1651
NaeI GCCGGC 1 cut(s) 1140
NciI CCSGG 2 cut(s) 80, 298
NcoI CCATGG 1 cut(s) 233
NgoMIV GCCGGC 1 cut(s) 1138
NlaIII CATG 3 cut(s) 237, 436, 1722
NlaIV GGNNCC 6 cut(s) 239, 528, 600, 882, 930, 1415
NmeAIII GCCGAG 3 cut(s) 288, 367, 1588
NmuCI GTSAC 4 cut(s) 847, 1036, 1214, 1483
NspV TTCGAA 1 cut(s) 975
OliI CACNNNNGTG 1 cut(s) 1466
PaeR7I CTCGAG 2 cut(s) 251, 440
PcsI WCGNNNNNNNCGW 7 cut(s) 249, 447, 456, 709, 753, 776, 881
PctI GAATGC 1 cut(s) 1489
PdiI GCCGGC 1 cut(s) 1140
PfeI GAWTC 7 cut(s) 212, 360, 657, 668, 772, 781, 1550
PfoI TCCNGGA 1 cut(s) 78
PinAI ACCGGT 1 cut(s) 931
Ple19I CGATCG 2 cut(s) 457, 945
Psp124BI GAGCTC 3 cut(s) 324, 732, 1244
Psp6I CCWGG 2 cut(s) 1397, 1444
PspGI CCWGG 2 cut(s) 1397, 1444
PspN4I GGNNCC 6 cut(s) 239, 528, 600, 882, 930, 1415
PspPI GGNCC 3 cut(s) 82, 170, 237
PspXI VCTCGAGB 1 cut(s) 251
PsrI GAACNNNNNNTAC 2 cut(s) 1301, 1333
PsuI RGATCY 1 cut(s) 673
PvuI CGATCG 2 cut(s) 457, 945
RsaI GTAC 7 cut(s) 5, 284, 331, 767, 1277, 1319, 1415
RsaNI GTAC 7 cut(s) 4, 283, 330, 766, 1276, 1318, 1414
RseI CAYNNNNRTG 2 cut(s) 434, 1466
Rsr2I CGGWCCG 1 cut(s) 82
RsrII CGGWCCG 1 cut(s) 82
SacI GAGCTC 3 cut(s) 324, 732, 1244
SaqAI TTAA 4 cut(s) 1065, 1233, 1404, 1536
Sau96I GGNCC 3 cut(s) 82, 170, 237
ScrFI CCNGG 4 cut(s) 80, 298, 1399, 1446
SduI GDGCHC 4 cut(s) 324, 732, 1244, 1473
SfaNI GCATC 2 cut(s) 300, 426
Sfr274I CTCGAG 2 cut(s) 251, 440
SfuI TTCGAA 1 cut(s) 975
SgrAI CRCCGGYG 3 cut(s) 467, 902, 1138
SinI GGWCC 2 cut(s) 82, 237
SlaI CTCGAG 2 cut(s) 251, 440
SmiMI CAYNNNNRTG 2 cut(s) 434, 1466
SmlI CTYRAG 3 cut(s) 251, 440, 725
SmoI CTYRAG 3 cut(s) 251, 440, 725
Sse9I AATT 7 cut(s) 491, 575, 977, 1029, 1047, 1322, 1690
SspMI CTAG 2 cut(s) 407, 531
SstI GAGCTC 3 cut(s) 324, 732, 1244
StyD4I CCNGG 4 cut(s) 78, 296, 1397, 1444
StyI CCWWGG 3 cut(s) 233, 530, 1101
TaaI ACNGT 3 cut(s) 86, 569, 853
TaiI ACGT 7 cut(s) 246, 450, 483, 516, 718, 738, 1319
TaqI TCGA 9 cut(s) 252, 441, 453, 483, 542, 660, 770, 975, 1238
TaqII GACCGA 1 cut(s) 1055
TasI AATT 7 cut(s) 491, 575, 977, 1029, 1047, 1322, 1690
TatI WGTACW 1 cut(s) 1275
TauI GCSGC 5 cut(s) 50, 53, 135, 205, 315
TfiI GAWTC 7 cut(s) 212, 360, 657, 668, 772, 781, 1550
Tru1I TTAA 4 cut(s) 1065, 1233, 1404, 1536
Tru9I TTAA 4 cut(s) 1065, 1233, 1404, 1536
TseFI GTSAC 4 cut(s) 847, 1036, 1214, 1483
TseI GCWGC 5 cut(s) 414, 1287, 1641, 1674, 1715
Tsp45I GTSAC 4 cut(s) 847, 1036, 1214, 1483
TspDTI ATGAA 2 cut(s) 1041, 1691
TspGWI ACGGA 4 cut(s) 208, 317, 391, 906
VpaK11BI GGWCC 2 cut(s) 82, 237
XapI RAATTY 1 cut(s) 1047
XhoI CTCGAG 2 cut(s) 251, 440
XmaJI CCTAGG 1 cut(s) 530
XspI CTAG 2 cut(s) 407, 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.