Rh1CG346900

amidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1C
OG0017136
Physical Location & Seq
Forward (+)
62395361 .. 62397214
1854 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1CG346900.1

Sequence Viewer

Length: 1854 bp
ATGAAATTCCCAATTTACCCGCGTTATTTTGAAGTCCCTACTCCTACTACCTCAAACACTAGCTCTCTCCGTCACTCCAACTCGGTCCAAATGTACGGCCTCCTCTCTCTCCTCCTCCTCCTCCTCCGCTTCTTCGCCGCCGCAGTCTCCGCCTCCTTCCCGGACCGTTTCACCTCCTCCTCTCTCCGGCGTCACAAATCCCAAGAATACTTCGAGCTCGGCCGTCCTCTGCCGTCCGATCTCCTCCCCCCGTCCTGCTACCGCCGCGTCCTCACCCACTCCTTCGCCAACACCATCAACGGCCCTCCCTTCTCCGCCCCATACTCCCCTCCGCCGCCCTCCGAATCCTCCGTTTGCTCCGCCCCATGGTCCCATGTCGCCCTCGAGTTTCGCGCCAAATGCAAAGGCGAGCAGTACGACCGCATCGCCGGGCTCTGGCTCGGCGGCGTTGAGCTCCTCCGTACCAGCACCGCCGAGCCGACCGAGAAGGGAATCTTCTGGAAGGTTCGCAAGGACATCACTAAATACTCCTCCGTCCTAGCTCGCTGCGATCTCAACCTCACCATGATGCTCGAGAACGTCGTCGATCGCGTCTTCACCGGCGTTTACCACGTCGAGGTTCATTTCCTCTATTACAAGGCAAACGTCGCCGTTTCGGAGCCTAGGGTTTCTTCGATCCTTGCAGATCATACTTTCAACGGTCAAAAATTGGGGATCGTTGTTCCCGAGGAGCCTGCCGATCTGATCGTTCCGATCTCCGACGACGGTGACCGAGGGTTTTGGTTCAGAATCGACGGCGAATCGGATTTCCCTTCCAAGCAAATCCAACTCCCTCGGAACACACGACGTGTCGTTCTTGAGCTCTATGTTTCGTTTCACGGCAACGACGAGTTCTGGTACTCGAATCCGCCGAATCTATACATCACGACGAACAATCTTACGACCGGACGAGGAAATGGAGCTTACAGAGAGGTTTTCGTGACGGTCGACGGCGAGTTGGTGGGATCGGAGGTGCCGTTTCCGGTGGTGTTCACCGGCGGGATCAATCCGTTGTTTTGGGAACCGGTGGTGGCCATCGGAGCTTTCAACCTTCCTTCTTATGATTTCGAATTGACGCCGTTTCTGGGGAAGCTTCTGGATAAGAAGGCTCATAGCTTTGGAATTGGAGTGACCGATGGAATTTCATATTGGCTTGTTAATGCCAACTTACACATTTGGTTGGATCATAAATCCAAGGCTGTGAAAGCTAAATCAAACTCGTTTCCTTCGCCGGCGATTGAAGTGAAGCGTAATGCTCAGTTCAAAAAGCTTGACGGGTCGTTCAGGATCAAGGCAAGGAGGAGGAGTGACTTTGTGGGGTGGGTTAAGTCGAGCTCAGGGAACTACACGACCACATTCTCGCAGAAGTACAAGTTCAGCAGCTCGGTTAGTTTTGGGAAGAAGGGGACGTACAAATTGGTGAACCAGAAGGTCAAGGCTGAGAAGGAAGTGAGGGTTGTGAGTGAGATTGGTGTGGTGGTTCTTAGAACTAGGGTTAAGGGAAGGTACCCTATTGATGTTTTTACAGCTACTCTACCGGGTAAGAAGAAAGACACCTATGTGCTCCTCACCAATGTCACGCATTCGGTGAAGGAGAGGAGCTGGCACGGGGGATATTTGAGCCGTGTTAACAACATTCAAGATTCAAGAGGGTGGATGGAGGTCAGGGATCATTCTGTTCTTTCTGGAACAGCAGACACTCGGCAGAGCTTCGGTTACAAAGATAACTTTGGCTGCTACTCGCGCACTATTGGCGCTGTTGATGGCAGCTTGATCAGGGATAATTCCAGCTTTCATTGCATATCTGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

617

Amino Acids

69.26

Weight (kDa)

9.44

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNGaseA PF12222 86 - 419 9.4e-92 Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
PNGase_A_C PF25156 440 - 497 1.7e-06 PNGase_A C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05480
fragaria_vesca FvH4_7g24990
prunus_persica Prupe.2G255000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0370891
rosa_laevigata RLG00000027001
rosa_multiflora Rmu_co8367915.1_g000001
rosa_roxburghii Rroxscaffold_4G00286150
rosa_rugosa Rorug01G0360200
rosa_samantha Rh1AG370000 Rh1BG333600 Rh1CG346900 Rh1DG364800
rosa_wichuraiana Rw1G032560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1545
AccB1I GGYRCC 2 cut(s) 1012, 1545
AccI GTMKAC 1 cut(s) 987
AccII CGCG 5 cut(s) 22, 267, 393, 591, 1783
AclWI GGATC 7 cut(s) 670, 722, 1012, 1049, 1230, 1334, 1716
AcoI YGGCCR 2 cut(s) 220, 1071
AcsI RAATTY 2 cut(s) 5, 1179
AcyI GRCGYC 2 cut(s) 190, 1115
AdeI CACNNNGTG 1 cut(s) 848
AfaI GTAC 7 cut(s) 95, 416, 463, 899, 1409, 1451, 1547
AfiI CCNNNNNNNGG 5 cut(s) 186, 366, 435, 616, 1124
AflIII ACRYGT 1 cut(s) 847
AgeI ACCGGT 1 cut(s) 1063
AgsI TTSAA 7 cut(s) 32, 697, 1087, 1280, 1303, 1679, 1686
AjiI CACGTC 2 cut(s) 613, 848
AjuI GAANNNNNNNTTGG 2 cut(s) 1171, 1203
AleI CACNNNNGTG 1 cut(s) 1598
Alw21I GWGCWC 5 cut(s) 219, 456, 864, 1376, 1605
Alw26I GTCTC 1 cut(s) 151
AlwI GGATC 7 cut(s) 670, 722, 1012, 1049, 1230, 1334, 1716
Ama87I CYCGRG 3 cut(s) 383, 572, 725
AoxI GGCC 4 cut(s) 97, 220, 301, 1071
ApeKI GCWGC 5 cut(s) 546, 1419, 1773, 1806, 1847
ApoI RAATTY 2 cut(s) 5, 1179
AsiGI ACCGGT 1 cut(s) 1063
Asp718I GGTACC 1 cut(s) 1545
AspA2I CCTAGG 1 cut(s) 662
AspLEI GCGC 3 cut(s) 395, 1785, 1796
AspS9I GGNCC 4 cut(s) 85, 163, 302, 369
AsuC2I CCSGG 3 cut(s) 161, 430, 1578
AsuHPI GGTGA 9 cut(s) 163, 265, 553, 589, 779, 1024, 1471, 1600, 1639
AsuII TTCGAA 1 cut(s) 1107
AvaI CYCGRG 3 cut(s) 383, 572, 725
AvaII GGWCC 3 cut(s) 85, 163, 369
AvrII CCTAGG 1 cut(s) 662
BalI TGGCCA 1 cut(s) 1073
BanI GGYRCC 2 cut(s) 1012, 1545
BanII GRGCYC 5 cut(s) 219, 435, 456, 864, 1376
BarI GAAGNNNNNNTAC 2 cut(s) 1433, 1465
BbsI GAAGAC 1 cut(s) 586
Bbv12I GWGCWC 5 cut(s) 219, 456, 864, 1376, 1605
BbvI GCAGC 5 cut(s) 533, 1431, 1760, 1818, 1834
BccI CCATC 5 cut(s) 302, 1082, 1169, 1690, 1796
BcgI CGANNNNNNTGC 2 cut(s) 716, 750
BclI TGATCA 1 cut(s) 1812
BcnI CCSGG 3 cut(s) 161, 430, 1578
BcoDI GTCTC 1 cut(s) 151
BfaI CTAG 4 cut(s) 60, 539, 663, 1530
BfoI RGCGCY 1 cut(s) 1797
BlnI CCTAGG 1 cut(s) 662
Bme1390I CCNGG 3 cut(s) 161, 430, 1578
Bme18I GGWCC 3 cut(s) 85, 163, 369
BmeT110I CYCGRG 3 cut(s) 383, 572, 725
BmgBI CACGTC 2 cut(s) 613, 848
BmgT120I GGNCC 4 cut(s) 85, 163, 302, 369
BmiI GGNNCC 6 cut(s) 371, 660, 732, 1014, 1062, 1547
BmrFI CCNGG 3 cut(s) 161, 430, 1578
BmsI GCATC 2 cut(s) 432, 558
BpiI GAAGAC 1 cut(s) 586
Bpu10I CCTNAGC 1 cut(s) 1375
Bpu14I TTCGAA 1 cut(s) 1107
BpuEI CTTGAG 1 cut(s) 878
BpuMI CCSGG 3 cut(s) 161, 430, 1578
BsaHI GRCGYC 2 cut(s) 190, 1115
BsaJI CCNNGG 6 cut(s) 365, 662, 726, 772, 833, 1233
BsaWI WCCGGW 3 cut(s) 944, 1021, 1063
BsaXI ACNNNNNCTCC 4 cut(s) 1001, 1031, 1330, 1360
Bsc4I CCNNNNNNNGG 5 cut(s) 186, 366, 435, 616, 1124
Bse118I RCCGGY 4 cut(s) 599, 1034, 1063, 1270
Bse3DI GCAATG 1 cut(s) 1834
BseDI CCNNGG 6 cut(s) 365, 662, 726, 772, 833, 1233
BseGI GGATG 1 cut(s) 1701
BseLI CCNNNNNNNGG 5 cut(s) 186, 366, 435, 616, 1124
BseMI GCAATG 1 cut(s) 1834
BseMII CTCAG 3 cut(s) 1310, 1389, 1470
BseX3I CGGCCG 1 cut(s) 220
BseXI GCAGC 5 cut(s) 533, 1431, 1760, 1818, 1834
Bsh1236I CGCG 5 cut(s) 22, 267, 393, 591, 1783
Bsh1285I CGRYCG 6 cut(s) 223, 421, 483, 589, 945, 987
BshFI GGCC 4 cut(s) 99, 222, 303, 1073
BshNI GGYRCC 2 cut(s) 1012, 1545
BshTI ACCGGT 1 cut(s) 1063
BsiEI CGRYCG 6 cut(s) 223, 421, 483, 589, 945, 987
BsiHKAI GWGCWC 5 cut(s) 219, 456, 864, 1376, 1605
BsiHKCI CYCGRG 3 cut(s) 383, 572, 725
BslFI GGGAC 3 cut(s) 20, 355, 1459
BslI CCNNNNNNNGG 5 cut(s) 186, 366, 435, 616, 1124
BsmAI GTCTC 1 cut(s) 151
BsmFI GGGAC 3 cut(s) 20, 355, 1459
BsmI GAATGC 1 cut(s) 1621
BsnI GGCC 4 cut(s) 99, 222, 303, 1073
BsoBI CYCGRG 3 cut(s) 383, 572, 725
Bsp119I TTCGAA 1 cut(s) 1107
Bsp1286I GDGCHC 6 cut(s) 219, 435, 456, 864, 1376, 1605
Bsp19I CCATGG 1 cut(s) 365
BspANI GGCC 4 cut(s) 99, 222, 303, 1073
BspCNI CTCAG 3 cut(s) 1309, 1388, 1471
BspFNI CGCG 5 cut(s) 22, 267, 393, 591, 1783
BspLI GGNNCC 6 cut(s) 371, 660, 732, 1014, 1062, 1547
BspPI GGATC 7 cut(s) 670, 722, 1012, 1049, 1230, 1334, 1716
BspT104I TTCGAA 1 cut(s) 1107
BspT107I GGYRCC 2 cut(s) 1012, 1545
BsrDI GCAATG 1 cut(s) 1834
BsrFI RCCGGY 4 cut(s) 599, 1034, 1063, 1270
BssAI RCCGGY 4 cut(s) 599, 1034, 1063, 1270
BssECI CCNNGG 6 cut(s) 365, 662, 726, 772, 833, 1233
BssNI GRCGYC 2 cut(s) 190, 1115
BssT1I CCWWGG 3 cut(s) 365, 662, 1233
Bst4CI ACNGT 4 cut(s) 167, 701, 767, 985
BstACI GRCGYC 2 cut(s) 190, 1115
BstBI TTCGAA 1 cut(s) 1107
BstC8I GCNNGC 5 cut(s) 410, 544, 735, 1272, 1643
BstDEI CTNAG 4 cut(s) 1296, 1375, 1479, 1523
BstDSI CCRYGG 1 cut(s) 365
BstEII GGTNACC 1 cut(s) 767
BstF5I GGATG 1 cut(s) 1701
BstFNI CGCG 5 cut(s) 22, 267, 393, 591, 1783
BstH2I RGCGCY 1 cut(s) 1797
BstHHI GCGC 3 cut(s) 395, 1785, 1796
BstMAI GTCTC 1 cut(s) 151
BstMCI CGRYCG 6 cut(s) 223, 421, 483, 589, 945, 987
BstMWI GCNNNNNNNGC 9 cut(s) 149, 264, 399, 647, 1079, 1244, 1782, 1791, 1836
BstPI GGTNACC 1 cut(s) 767
BstSCI CCNGG 3 cut(s) 159, 428, 1576
BstUI CGCG 5 cut(s) 22, 267, 393, 591, 1783
BstV1I GCAGC 5 cut(s) 533, 1431, 1760, 1818, 1834
BstV2I GAAGAC 1 cut(s) 586
BstZI CGGCCG 1 cut(s) 220
BsuRI GGCC 4 cut(s) 99, 222, 303, 1073
BtgI CCRYGG 1 cut(s) 365
BtgZI GCGATG 1 cut(s) 409
BtrI CACGTC 2 cut(s) 613, 848
BtsCI GGATG 1 cut(s) 1701
Cac8I GCNNGC 5 cut(s) 410, 544, 735, 1272, 1643
CfoI GCGC 3 cut(s) 395, 1785, 1796
Cfr10I RCCGGY 4 cut(s) 599, 1034, 1063, 1270
Cfr13I GGNCC 4 cut(s) 85, 163, 302, 369
CpoI CGGWCCG 1 cut(s) 163
CseI GACGC 4 cut(s) 179, 256, 580, 1123
Csp6I GTAC 7 cut(s) 94, 415, 462, 898, 1408, 1450, 1546
CspAI ACCGGT 1 cut(s) 1063
CspI CGGWCCG 1 cut(s) 163
CviAII CATG 4 cut(s) 366, 374, 565, 1851
CviQI GTAC 7 cut(s) 94, 415, 462, 898, 1408, 1450, 1546
DdeI CTNAG 4 cut(s) 1296, 1375, 1479, 1523
DraIII CACNNNGTG 1 cut(s) 848
EaeI YGGCCR 2 cut(s) 220, 1071
EagI CGGCCG 1 cut(s) 220
EciI GGCGGA 5 cut(s) 139, 304, 321, 349, 897
Ecl136II GAGCTC 4 cut(s) 217, 454, 862, 1374
EclXI CGGCCG 1 cut(s) 220
Eco130I CCWWGG 3 cut(s) 365, 662, 1233
Eco24I GRGCYC 5 cut(s) 219, 435, 456, 864, 1376
Eco47I GGWCC 3 cut(s) 85, 163, 369
Eco52I CGGCCG 1 cut(s) 220
Eco53kI GAGCTC 4 cut(s) 217, 454, 862, 1374
Eco88I CYCGRG 3 cut(s) 383, 572, 725
Eco91I GGTNACC 1 cut(s) 767
EcoICRI GAGCTC 4 cut(s) 217, 454, 862, 1374
EcoO65I GGTNACC 1 cut(s) 767
EcoT14I CCWWGG 3 cut(s) 365, 662, 1233
EcoT38I GRGCYC 5 cut(s) 219, 435, 456, 864, 1376
ErhI CCWWGG 3 cut(s) 365, 662, 1233
FaeI CATG 4 cut(s) 369, 377, 568, 1854
FalI AAGNNNNNCTT 2 cut(s) 479, 511
FaqI GGGAC 3 cut(s) 20, 355, 1459
FatI CATG 4 cut(s) 365, 373, 564, 1850
FauI CCCGC 2 cut(s) 27, 1031
FbaI TGATCA 1 cut(s) 1812
FblI GTMKAC 1 cut(s) 987
FokI GGATG 1 cut(s) 1708
FriOI GRGCYC 5 cut(s) 219, 435, 456, 864, 1376
FspBI CTAG 4 cut(s) 60, 539, 663, 1530
GlaI GCGC 3 cut(s) 394, 1784, 1795
HaeII RGCGCY 1 cut(s) 1797
HaeIII GGCC 4 cut(s) 99, 222, 303, 1073
HgaI GACGC 4 cut(s) 179, 256, 580, 1123
HhaI GCGC 3 cut(s) 395, 1785, 1796
Hin1I GRCGYC 2 cut(s) 190, 1115
Hin1II CATG 4 cut(s) 369, 377, 568, 1854
Hin6I GCGC 3 cut(s) 393, 1783, 1794
HinP1I GCGC 3 cut(s) 393, 1783, 1794
HincII GTYRAC 2 cut(s) 988, 1669
HindII GTYRAC 2 cut(s) 988, 1669
HindIII AAGCTT 2 cut(s) 1130, 1307
HinfI GANTC 7 cut(s) 344, 492, 789, 800, 904, 913, 1682
HpaI GTTAAC 1 cut(s) 1669
HphI GGTGA 9 cut(s) 163, 265, 553, 589, 779, 1024, 1471, 1600, 1639
Hpy166II GTNNAC 5 cut(s) 607, 988, 1032, 1462, 1669
Hpy8I GTNNAC 5 cut(s) 607, 988, 1032, 1462, 1669
HpyCH4III ACNGT 4 cut(s) 167, 701, 767, 985
HpyCH4IV ACGT 5 cut(s) 579, 612, 645, 847, 1448
HpyCH4V TGCA 4 cut(s) 402, 683, 1839, 1850
HpyF10VI GCNNNNNNNGC 9 cut(s) 149, 264, 399, 647, 1079, 1244, 1782, 1791, 1836
HpyF3I CTNAG 4 cut(s) 1296, 1375, 1479, 1523
HpySE526I ACGT 5 cut(s) 579, 612, 645, 847, 1448
Hsp92I GRCGYC 2 cut(s) 190, 1115
Hsp92II CATG 4 cut(s) 369, 377, 568, 1854
HspAI GCGC 3 cut(s) 393, 1783, 1794
KpnI GGTACC 1 cut(s) 1549
KroI GCCGGC 1 cut(s) 1270
KroNI GCCGGC 1 cut(s) 1272
Ksp22I TGATCA 1 cut(s) 1812
KspAI GTTAAC 1 cut(s) 1669
LmnI GCTCC 8 cut(s) 362, 459, 658, 730, 959, 1079, 1608, 1638
Lsp1109I GCAGC 5 cut(s) 533, 1431, 1760, 1818, 1834
LweI GCATC 2 cut(s) 432, 558
MaeI CTAG 4 cut(s) 60, 539, 663, 1530
MaeII ACGT 5 cut(s) 579, 612, 645, 847, 1448
MaeIII GTNAC 8 cut(s) 71, 191, 767, 979, 1168, 1346, 1615, 1754
MboII GAAGA 6 cut(s) 124, 487, 586, 663, 1450, 1597
MhlI GDGCHC 6 cut(s) 219, 435, 456, 864, 1376, 1605
MlsI TGGCCA 1 cut(s) 1073
MluCI AATT 8 cut(s) 5, 12, 707, 1109, 1161, 1179, 1454, 1822
MluNI TGGCCA 1 cut(s) 1073
MmeI TCCRAC 4 cut(s) 102, 783, 850, 1200
Mox20I TGGCCA 1 cut(s) 1073
MreI CGCCGGCG 1 cut(s) 1270
MroNI GCCGGC 1 cut(s) 1270
MscI TGGCCA 1 cut(s) 1073
MseI TTAA 4 cut(s) 1197, 1365, 1536, 1668
MslI CAYNNNNRTG 2 cut(s) 566, 1598
Msp20I TGGCCA 1 cut(s) 1073
MspR9I CCNGG 3 cut(s) 161, 430, 1578
Mva1269I GAATGC 1 cut(s) 1621
MvnI CGCG 5 cut(s) 22, 267, 393, 591, 1783
MwoI GCNNNNNNNGC 9 cut(s) 149, 264, 399, 647, 1079, 1244, 1782, 1791, 1836
NaeI GCCGGC 1 cut(s) 1272
NciI CCSGG 3 cut(s) 161, 430, 1578
NcoI CCATGG 1 cut(s) 365
NgoMIV GCCGGC 1 cut(s) 1270
NlaIII CATG 4 cut(s) 369, 377, 568, 1854
NlaIV GGNNCC 6 cut(s) 371, 660, 732, 1014, 1062, 1547
NmeAIII GCCGAG 4 cut(s) 198, 420, 499, 1720
NmuCI GTSAC 7 cut(s) 71, 191, 767, 979, 1168, 1346, 1615
NspV TTCGAA 1 cut(s) 1107
OliI CACNNNNGTG 1 cut(s) 1598
PaeR7I CTCGAG 2 cut(s) 383, 572
PcsI WCGNNNNNNNCGW 8 cut(s) 579, 588, 723, 841, 885, 908, 984, 1013
PctI GAATGC 1 cut(s) 1621
PdiI GCCGGC 1 cut(s) 1272
PfeI GAWTC 7 cut(s) 344, 492, 789, 800, 904, 913, 1682
PfoI TCCNGGA 1 cut(s) 159
PinAI ACCGGT 1 cut(s) 1063
Ple19I CGATCG 1 cut(s) 589
Psp124BI GAGCTC 4 cut(s) 219, 456, 864, 1376
PspEI GGTNACC 1 cut(s) 767
PspN4I GGNNCC 6 cut(s) 371, 660, 732, 1014, 1062, 1547
PspPI GGNCC 4 cut(s) 85, 163, 302, 369
PspXI VCTCGAGB 1 cut(s) 383
PvuI CGATCG 1 cut(s) 589
RsaI GTAC 7 cut(s) 95, 416, 463, 899, 1409, 1451, 1547
RsaNI GTAC 7 cut(s) 94, 415, 462, 898, 1408, 1450, 1546
RseI CAYNNNNRTG 2 cut(s) 566, 1598
Rsr2I CGGWCCG 1 cut(s) 163
RsrII CGGWCCG 1 cut(s) 163
SacI GAGCTC 4 cut(s) 219, 456, 864, 1376
SalI GTCGAC 1 cut(s) 986
SaqAI TTAA 4 cut(s) 1197, 1365, 1536, 1668
Sau96I GGNCC 4 cut(s) 85, 163, 302, 369
ScrFI CCNGG 3 cut(s) 161, 430, 1578
SduI GDGCHC 6 cut(s) 219, 435, 456, 864, 1376, 1605
SfaNI GCATC 2 cut(s) 432, 558
Sfr274I CTCGAG 2 cut(s) 383, 572
SfuI TTCGAA 1 cut(s) 1107
SgrAI CRCCGGYG 3 cut(s) 599, 1034, 1270
SinI GGWCC 3 cut(s) 85, 163, 369
SlaI CTCGAG 2 cut(s) 383, 572
SmiMI CAYNNNNRTG 2 cut(s) 566, 1598
SmlI CTYRAG 3 cut(s) 383, 572, 857
SmoI CTYRAG 3 cut(s) 383, 572, 857
Sse9I AATT 8 cut(s) 5, 12, 707, 1109, 1161, 1179, 1454, 1822
SspMI CTAG 4 cut(s) 60, 539, 663, 1530
SstI GAGCTC 4 cut(s) 219, 456, 864, 1376
StyD4I CCNGG 3 cut(s) 159, 428, 1576
StyI CCWWGG 3 cut(s) 365, 662, 1233
TaaI ACNGT 4 cut(s) 167, 701, 767, 985
TaiI ACGT 5 cut(s) 582, 615, 648, 850, 1451
TaqII GACCGA 4 cut(s) 73, 497, 786, 1187
TasI AATT 8 cut(s) 5, 12, 707, 1109, 1161, 1179, 1454, 1822
TatI WGTACW 1 cut(s) 1407
TauI GCSGC 5 cut(s) 140, 143, 267, 337, 447
TfiI GAWTC 7 cut(s) 344, 492, 789, 800, 904, 913, 1682
Tru1I TTAA 4 cut(s) 1197, 1365, 1536, 1668
Tru9I TTAA 4 cut(s) 1197, 1365, 1536, 1668
TseFI GTSAC 7 cut(s) 71, 191, 767, 979, 1168, 1346, 1615
TseI GCWGC 5 cut(s) 546, 1419, 1773, 1806, 1847
Tsp45I GTSAC 7 cut(s) 71, 191, 767, 979, 1168, 1346, 1615
TspDTI ATGAA 4 cut(s) 17, 611, 1173, 1823
TspGWI ACGGA 5 cut(s) 59, 340, 449, 523, 1038
VpaK11BI GGWCC 3 cut(s) 85, 163, 369
XapI RAATTY 2 cut(s) 5, 1179
XhoI CTCGAG 2 cut(s) 383, 572
XmaJI CCTAGG 1 cut(s) 662
XmiI GTMKAC 1 cut(s) 987
XspI CTAG 4 cut(s) 60, 539, 663, 1530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.