FvH4_7g24990

amidase A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0017136
Physical Location & Seq
Forward (+)
19166555 .. 19168306
1752 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24990.t1

Sequence Viewer

Length: 1752 bp
ATGTACGGCCTCCTCTTCCTCCTGCTCCTCTTCTCCACCGCCACCTCCTCCCCGGACCGCTTCACCTCCTCCTCTCTCCTCCGTCACAAATCCAAAGAATACTTCGAGCTCGGCCGTCCTCTCCCCTCCGATCTCCTCCCCCTGTCCTGCTCCCGCCACGTCCTCACCCACTCCTTCGCCAACACCATCAACGGCCCTCCCTTCTCCTCCCCCTACTCCCCTCCACCGCCGTCCTCCTCCGACTGCTCCTTCGACTGCTCTCCCCCTTGGTCCCACGTCGCCCTCGAGTTTCGCGCCAAATGCAAAGGCGAGCAGTACGACCGCATCGCCGCCCTCTGGCTCGGCGGCGTCGAGCTCCTCCGTACAAGCACCGCCGAGCCGAACAAGAAAGGAATCTTCTGGAAGGTTCGCAAGGACATAACGAAGTACTCCTCCCTCTTAACCCGCTCCGATCTAAACCTCACCATGATGCTCGAGAACGTCGTCAACCGCGTCTTCACCGGCGTCTACCACGTCGAGGTTGAGTTTCTCTATTACAAGCAATACGTCGCCGTTTCGGAGCCTAGGGTTTCCTCGATCCTCGTGGATCAAAATCTCGACGGCCGAAATTTGAGGATCGTTCCTGAGGAGCCTGCCGATTTGATCGTCCCGATCACCGACGACGGCGACCGAGGGTTCTGGTTCAGAATCGAAAGCGAAGCGGATTTCCCTTCCAGGCAAATCCGAATCCCTCGGAACACACGACGTGTCGTACTCGAGCTCTACGTCTCGTTTCACGGCGACGACGAGTTCTGGTACTCGAATCCGCCGAATCTGTACATCACGACGAACAATTTGACCACCGGAAGAGGAAACGGAGCTTACCGGGAGGTTTTCGTGACCGTCGACGGCGAGCTGGTGGGGTCGGAGGTGCCGTTTCCGGTGGTGTTCACCGGCGGGATCAATCCTCTGTTTTGGGAGCCGGTGGTGGCCATCGGAGCTTTCAACCTGCCGTCTTATGATTTCGAATTGACTCCATTTCTGGGGAAGCTTCTGGATAAGAAGCCTCATAGCTTTGGGATTGGAGTGAGTGATGGAATTTCATACTGGCTTCTAAATGCCAACTTACACATTTGGCTGGATCATAAATCCAAGACTGTGCATGCTAAATCAAGCTTCTTTCCTTTGCCGGCGATCCAGCTGAAGCGTAATGCCCAGTTCAAATTGCTGGACGGATCGTTCAACATCAAGGCGCAGAGGAGGACTGACTTTGTGGGGTGGGTTTTGTCGAGCTCAGGCAATCACACCACTATGTTTTCGCAGAAGTACAAGTTCACGAGCTCGATTAGCTTCGCGAGGAACGGGACTTACAAGATGGTGAAGCAGAAGGTGAAGGCTGATAAGGAAGTGAAGGTTGTGAGGGAGGATGGTGTGGTGGTTCTTAGGACTAGGGCGAAGAGGAGGTACCCTATTGATGTTATTACAGCCACTCTACCGGGTAAGAAGAAAAATACCTATGTGCTTGTCACCAATGTGACGCATTCGATGAAGGAGAAGAGCTTGCATGGGGGGTATCTGAGCCGCGTTAACAACTTTCAGGATTCGAGAGGGTGGATGGAGGTCAGGGATCATGATGTGCTTTCCGGAACTGCAGACACGCAGCAGAGTTTCGGATACAGAGATGAGTTTGGCTGCTACTCGCGCACTATTGGCGTTGTCGATGGCAGCCTGACGAGGGATAATTCCAGCTTCAATTGTCTGTCTACTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PNGaseA

Protein Analysis

584

Amino Acids

65.9

Weight (kDa)

9.13

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PNGaseA PF12222 49 - 383 1.3e-91 Peptide N-acetyl-beta-D-glucosaminyl asparaginase amidase A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017136)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G05480
fragaria_vesca FvH4_7g24990
prunus_persica Prupe.2G255000_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0370891
rosa_laevigata RLG00000027001
rosa_multiflora Rmu_co8367915.1_g000001
rosa_roxburghii Rroxscaffold_4G00286150
rosa_rugosa Rorug01G0360200
rosa_samantha Rh1AG370000 Rh1BG333600 Rh1CG346900 Rh1DG364800
rosa_wichuraiana Rw1G032560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 996
Acc65I GGTACC 1 cut(s) 1443
AccB1I GGYRCC 2 cut(s) 910, 1443
AccBSI CCGCTC 1 cut(s) 447
AccI GTMKAC 3 cut(s) 507, 885, 1742
AccII CGCG 5 cut(s) 294, 492, 1334, 1563, 1681
AccIII TCCGGA 1 cut(s) 1622
AclWI GGATC 8 cut(s) 571, 594, 623, 947, 1128, 1168, 1222, 1614
AcoI YGGCCR 3 cut(s) 112, 601, 969
AcsI RAATTY 2 cut(s) 607, 1077
AcuI CTGAAG 1 cut(s) 1202
AcyI GRCGYC 2 cut(s) 348, 504
AdeI CACNNNGTG 1 cut(s) 746
AfaI GTAC 9 cut(s) 5, 317, 364, 428, 753, 797, 818, 1307, 1445
AfiI CCNNNNNNNGG 4 cut(s) 336, 517, 1022, 1714
AflIII ACRYGT 1 cut(s) 745
AgsI TTSAA 4 cut(s) 985, 1201, 1222, 1732
AjiI CACGTC 4 cut(s) 160, 277, 514, 746
AjnI CCWGG 1 cut(s) 713
AjuI GAANNNNNNNTTGG 2 cut(s) 86, 118
AleI CACNNNNGTG 1 cut(s) 1511
AloI GAACNNNNNNTCC 2 cut(s) 1202, 1234
Alw21I GWGCWC 5 cut(s) 111, 357, 762, 1274, 1322
Alw26I GTCTC 1 cut(s) 772
AlwI GGATC 8 cut(s) 571, 594, 623, 947, 1128, 1168, 1222, 1614
Ama87I CYCGRG 3 cut(s) 284, 473, 755
Aor13HI TCCGGA 1 cut(s) 1622
AoxI GGCC 5 cut(s) 7, 112, 193, 601, 969
ApeKI GCWGC 3 cut(s) 1639, 1671, 1704
ApoI RAATTY 2 cut(s) 607, 1077
ArsI GACNNNNNNTTYG 4 cut(s) 233, 265, 817, 849
Asp718I GGTACC 1 cut(s) 1443
AspA2I CCTAGG 1 cut(s) 563
AspLEI GCGC 3 cut(s) 296, 1234, 1683
AspS9I GGNCC 3 cut(s) 55, 194, 270
AsuC2I CCSGG 3 cut(s) 53, 866, 1476
AsuHPI GGTGA 9 cut(s) 55, 157, 454, 490, 646, 922, 1369, 1381, 1498
AsuII TTCGAA 1 cut(s) 1005
AvaI CYCGRG 3 cut(s) 284, 473, 755
AvaII GGWCC 2 cut(s) 55, 270
AvrII CCTAGG 1 cut(s) 563
AxyI CCTNAGG 1 cut(s) 624
BalI TGGCCA 1 cut(s) 971
BanI GGYRCC 2 cut(s) 910, 1443
BanII GRGCYC 5 cut(s) 111, 357, 762, 1274, 1322
BarI GAAGNNNNNNTAC 2 cut(s) 1427, 1459
BauI CACGAG 2 cut(s) 581, 1315
BbsI GAAGAC 1 cut(s) 487
Bbv12I GWGCWC 5 cut(s) 111, 357, 762, 1274, 1322
BbvI GCAGC 3 cut(s) 1651, 1658, 1716
BccI CCATC 7 cut(s) 194, 980, 1067, 1348, 1400, 1588, 1694
BciT130I CCWGG 1 cut(s) 715
BciVI GTATCC 1 cut(s) 1646
BcnI CCSGG 3 cut(s) 53, 866, 1476
BcoDI GTCTC 1 cut(s) 772
BfaI CTAG 2 cut(s) 564, 1428
BfmI CTRYAG 1 cut(s) 1629
BfuAI ACCTGC 1 cut(s) 996
BfuI GTATCC 1 cut(s) 1646
BisI GCNGC 6 cut(s) 330, 346, 1561, 1640, 1672, 1705
BlnI CCTAGG 1 cut(s) 563
BlsI GCNGC 6 cut(s) 331, 347, 1562, 1641, 1673, 1706
BmcAI AGTACT 1 cut(s) 428
Bme1390I CCNGG 4 cut(s) 53, 715, 866, 1476
Bme18I GGWCC 2 cut(s) 55, 270
BmeT110I CYCGRG 3 cut(s) 284, 473, 755
BmgBI CACGTC 4 cut(s) 160, 277, 514, 746
BmgT120I GGNCC 3 cut(s) 55, 194, 270
BmiI GGNNCC 6 cut(s) 272, 561, 630, 912, 960, 1445
BmrFI CCNGG 4 cut(s) 53, 715, 866, 1476
BmrI ACTGGG 1 cut(s) 1189
BmsI GCATC 2 cut(s) 333, 459
BmuI ACTGGG 1 cut(s) 1189
BpiI GAAGAC 1 cut(s) 487
Bpu10I CCTNAGC 1 cut(s) 1273
Bpu14I TTCGAA 1 cut(s) 1005
BpuMI CCSGG 3 cut(s) 53, 866, 1476
BsaBI GATNNNNATC 1 cut(s) 591
BsaHI GRCGYC 2 cut(s) 348, 504
BsaJI CCNNGG 5 cut(s) 51, 266, 563, 670, 731
BsaWI WCCGGW 3 cut(s) 842, 919, 1622
BsaXI ACNNNNNCTCC 2 cut(s) 899, 929
Bsc4I CCNNNNNNNGG 4 cut(s) 336, 517, 1022, 1714
Bse118I RCCGGY 4 cut(s) 500, 932, 961, 1168
Bse1I ACTGG 2 cut(s) 1091, 1195
Bse21I CCTNAGG 1 cut(s) 624
Bse8I GATNNNNATC 1 cut(s) 591
BseAI TCCGGA 1 cut(s) 1622
BseBI CCWGG 1 cut(s) 715
BseDI CCNNGG 5 cut(s) 51, 266, 563, 670, 731
BseGI GGATG 2 cut(s) 1411, 1599
BseJI GATNNNNATC 1 cut(s) 591
BseLI CCNNNNNNNGG 4 cut(s) 336, 517, 1022, 1714
BseMII CTCAG 3 cut(s) 615, 1287, 1547
BseNI ACTGG 2 cut(s) 1091, 1195
BseX3I CGGCCG 2 cut(s) 112, 601
BseXI GCAGC 3 cut(s) 1651, 1658, 1716
Bsh1236I CGCG 5 cut(s) 294, 492, 1334, 1563, 1681
Bsh1285I CGRYCG 4 cut(s) 115, 322, 604, 670
BshFI GGCC 5 cut(s) 9, 114, 195, 603, 971
BshNI GGYRCC 2 cut(s) 910, 1443
BsiEI CGRYCG 4 cut(s) 115, 322, 604, 670
BsiHKAI GWGCWC 5 cut(s) 111, 357, 762, 1274, 1322
BsiHKCI CYCGRG 3 cut(s) 284, 473, 755
BslFI GGGAC 3 cut(s) 256, 632, 1357
BslI CCNNNNNNNGG 4 cut(s) 336, 517, 1022, 1714
BsmAI GTCTC 1 cut(s) 772
BsmBI CGTCTC 1 cut(s) 772
BsmFI GGGAC 3 cut(s) 256, 632, 1357
BsmI GAATGC 1 cut(s) 1519
BsnI GGCC 5 cut(s) 9, 114, 195, 603, 971
BsoBI CYCGRG 3 cut(s) 284, 473, 755
Bsp119I TTCGAA 1 cut(s) 1005
Bsp1286I GDGCHC 5 cut(s) 111, 357, 762, 1274, 1322
Bsp13I TCCGGA 1 cut(s) 1622
Bsp1407I TGTACA 1 cut(s) 816
Bsp68I TCGCGA 1 cut(s) 1334
BspANI GGCC 5 cut(s) 9, 114, 195, 603, 971
BspCNI CTCAG 3 cut(s) 616, 1286, 1548
BspEI TCCGGA 1 cut(s) 1622
BspFNI CGCG 5 cut(s) 294, 492, 1334, 1563, 1681
BspHI TCATGA 1 cut(s) 1609
BspLI GGNNCC 6 cut(s) 272, 561, 630, 912, 960, 1445
BspMAI CTGCAG 1 cut(s) 1633
BspMI ACCTGC 1 cut(s) 996
BspPI GGATC 8 cut(s) 571, 594, 623, 947, 1128, 1168, 1222, 1614
BspQI GCTCTTC 1 cut(s) 1529
BspT104I TTCGAA 1 cut(s) 1005
BspT107I GGYRCC 2 cut(s) 910, 1443
BsrBI CCGCTC 1 cut(s) 447
BsrFI RCCGGY 4 cut(s) 500, 932, 961, 1168
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 2 cut(s) 1091, 1195
BssAI RCCGGY 4 cut(s) 500, 932, 961, 1168
BssECI CCNNGG 5 cut(s) 51, 266, 563, 670, 731
BssNI GRCGYC 2 cut(s) 348, 504
BssSI CACGAG 2 cut(s) 581, 1315
BssT1I CCWWGG 2 cut(s) 266, 563
Bst2BI CACGAG 2 cut(s) 581, 1315
Bst2UI CCWGG 1 cut(s) 715
Bst4CI ACNGT 2 cut(s) 883, 1138
Bst6I CTCTTC 5 cut(s) 20, 35, 841, 1430, 1529
BstACI GRCGYC 2 cut(s) 348, 504
BstAUI TGTACA 1 cut(s) 816
BstBI TTCGAA 1 cut(s) 1005
BstC8I GCNNGC 6 cut(s) 311, 633, 893, 1143, 1170, 1541
BstDEI CTNAG 4 cut(s) 624, 1273, 1421, 1556
BstF5I GGATG 2 cut(s) 1411, 1599
BstFNI CGCG 5 cut(s) 294, 492, 1334, 1563, 1681
BstHHI GCGC 3 cut(s) 296, 1234, 1683
BstMAI GTCTC 1 cut(s) 772
BstMCI CGRYCG 4 cut(s) 115, 322, 604, 670
BstMWI GCNNNNNNNGC 5 cut(s) 300, 977, 1326, 1680, 1689
BstNI CCWGG 1 cut(s) 715
BstNSI RCATGY 1 cut(s) 1145
BstSCI CCNGG 4 cut(s) 51, 713, 864, 1474
BstSFI CTRYAG 1 cut(s) 1629
BstUI CGCG 5 cut(s) 294, 492, 1334, 1563, 1681
BstV1I GCAGC 3 cut(s) 1651, 1658, 1716
BstV2I GAAGAC 1 cut(s) 487
BstZI CGGCCG 2 cut(s) 112, 601
Bsu36I CCTNAGG 1 cut(s) 624
BsuI GTATCC 1 cut(s) 1646
BsuRI GGCC 5 cut(s) 9, 114, 195, 603, 971
BtgZI GCGATG 1 cut(s) 310
BtrI CACGTC 4 cut(s) 160, 277, 514, 746
BtsCI GGATG 2 cut(s) 1411, 1599
BtuMI TCGCGA 1 cut(s) 1334
BveI ACCTGC 1 cut(s) 996
Cac8I GCNNGC 6 cut(s) 311, 633, 893, 1143, 1170, 1541
CciI TCATGA 1 cut(s) 1609
CfoI GCGC 3 cut(s) 296, 1234, 1683
Cfr10I RCCGGY 4 cut(s) 500, 932, 961, 1168
Cfr13I GGNCC 3 cut(s) 55, 194, 270
CpoI CGGWCCG 1 cut(s) 55
CseI GACGC 4 cut(s) 337, 481, 493, 1525
Csp6I GTAC 9 cut(s) 4, 316, 363, 427, 752, 796, 817, 1306, 1444
CspI CGGWCCG 1 cut(s) 55
CviAII CATG 5 cut(s) 466, 1142, 1544, 1610, 1749
CviQI GTAC 9 cut(s) 4, 316, 363, 427, 752, 796, 817, 1306, 1444
DdeI CTNAG 4 cut(s) 624, 1273, 1421, 1556
DraIII CACNNNGTG 1 cut(s) 746
EaeI YGGCCR 3 cut(s) 112, 601, 969
EagI CGGCCG 2 cut(s) 112, 601
Eam1104I CTCTTC 5 cut(s) 20, 35, 841, 1430, 1529
EarI CTCTTC 5 cut(s) 20, 35, 841, 1430, 1529
EciI GGCGGA 1 cut(s) 795
Ecl136II GAGCTC 5 cut(s) 109, 355, 760, 1272, 1320
EclXI CGGCCG 2 cut(s) 112, 601
Eco130I CCWWGG 2 cut(s) 266, 563
Eco24I GRGCYC 5 cut(s) 111, 357, 762, 1274, 1322
Eco47I GGWCC 2 cut(s) 55, 270
Eco52I CGGCCG 2 cut(s) 112, 601
Eco53kI GAGCTC 5 cut(s) 109, 355, 760, 1272, 1320
Eco57I CTGAAG 1 cut(s) 1202
Eco81I CCTNAGG 1 cut(s) 624
Eco88I CYCGRG 3 cut(s) 284, 473, 755
EcoICRI GAGCTC 5 cut(s) 109, 355, 760, 1272, 1320
EcoRII CCWGG 1 cut(s) 713
EcoT14I CCWWGG 2 cut(s) 266, 563
EcoT38I GRGCYC 5 cut(s) 111, 357, 762, 1274, 1322
ErhI CCWWGG 2 cut(s) 266, 563
Esp3I CGTCTC 1 cut(s) 772
FaeI CATG 5 cut(s) 469, 1145, 1547, 1613, 1752
FaqI GGGAC 3 cut(s) 256, 632, 1357
FatI CATG 5 cut(s) 465, 1141, 1543, 1609, 1748
FauI CCCGC 3 cut(s) 161, 452, 929
FblI GTMKAC 3 cut(s) 507, 885, 1742
Fnu4HI GCNGC 6 cut(s) 330, 346, 1561, 1640, 1672, 1705
FokI GGATG 2 cut(s) 1418, 1606
FriOI GRGCYC 5 cut(s) 111, 357, 762, 1274, 1322
Fsp4HI GCNGC 6 cut(s) 330, 346, 1561, 1640, 1672, 1705
FspBI CTAG 2 cut(s) 564, 1428
GlaI GCGC 3 cut(s) 295, 1233, 1682
GluI GCNGC 6 cut(s) 330, 346, 1561, 1640, 1672, 1705
HaeIII GGCC 5 cut(s) 9, 114, 195, 603, 971
HgaI GACGC 4 cut(s) 337, 481, 493, 1525
HhaI GCGC 3 cut(s) 296, 1234, 1683
Hin1I GRCGYC 2 cut(s) 348, 504
Hin1II CATG 5 cut(s) 469, 1145, 1547, 1613, 1752
Hin6I GCGC 3 cut(s) 294, 1232, 1681
HinP1I GCGC 3 cut(s) 294, 1232, 1681
HincII GTYRAC 3 cut(s) 487, 886, 1567
HindII GTYRAC 3 cut(s) 487, 886, 1567
HindIII AAGCTT 2 cut(s) 1028, 1153
HinfI GANTC 7 cut(s) 393, 687, 726, 802, 811, 1012, 1580
HpaI GTTAAC 1 cut(s) 1567
HphI GGTGA 9 cut(s) 55, 157, 454, 490, 646, 922, 1369, 1381, 1498
Hpy166II GTNNAC 7 cut(s) 487, 508, 886, 930, 1314, 1567, 1743
Hpy8I GTNNAC 7 cut(s) 487, 508, 886, 930, 1314, 1567, 1743
HpyAV CCTTC 9 cut(s) 184, 211, 259, 397, 720, 1360, 1366, 1384, 1522
HpyCH4III ACNGT 2 cut(s) 883, 1138
HpyCH4IV ACGT 7 cut(s) 159, 276, 480, 513, 546, 745, 765
HpyCH4V TGCA 5 cut(s) 303, 1141, 1543, 1631, 1748
HpyF10VI GCNNNNNNNGC 5 cut(s) 300, 977, 1326, 1680, 1689
HpyF3I CTNAG 4 cut(s) 624, 1273, 1421, 1556
HpySE526I ACGT 7 cut(s) 159, 276, 480, 513, 546, 745, 765
Hsp92I GRCGYC 2 cut(s) 348, 504
Hsp92II CATG 5 cut(s) 469, 1145, 1547, 1613, 1752
HspAI GCGC 3 cut(s) 294, 1232, 1681
Kpn2I TCCGGA 1 cut(s) 1622
KpnI GGTACC 1 cut(s) 1447
KroI GCCGGC 1 cut(s) 1168
KroNI GCCGGC 1 cut(s) 1170
KspAI GTTAAC 1 cut(s) 1567
LguI GCTCTTC 1 cut(s) 1529
Lsp1109I GCAGC 3 cut(s) 1651, 1658, 1716
LweI GCATC 2 cut(s) 333, 459
MaeI CTAG 2 cut(s) 564, 1428
MaeII ACGT 7 cut(s) 159, 276, 480, 513, 546, 745, 765
MaeIII GTNAC 4 cut(s) 83, 877, 1504, 1513
MbiI CCGCTC 1 cut(s) 447
MboII GAAGA 8 cut(s) 7, 22, 388, 487, 858, 1447, 1495, 1546
MfeI CAATTG 1 cut(s) 1732
MhlI GDGCHC 5 cut(s) 111, 357, 762, 1274, 1322
MlsI TGGCCA 1 cut(s) 971
MluCI AATT 7 cut(s) 607, 832, 1007, 1077, 1202, 1720, 1732
MluNI TGGCCA 1 cut(s) 971
MlyI GAGTC 1 cut(s) 1006
MmeI TCCRAC 2 cut(s) 264, 885
Mox20I TGGCCA 1 cut(s) 971
MroI TCCGGA 1 cut(s) 1622
MroNI GCCGGC 1 cut(s) 1168
MscI TGGCCA 1 cut(s) 971
MseI TTAA 2 cut(s) 440, 1566
MslI CAYNNNNRTG 3 cut(s) 467, 1289, 1511
Msp20I TGGCCA 1 cut(s) 971
MspA1I CMGCKG 1 cut(s) 1180
MspR9I CCNGG 4 cut(s) 53, 715, 866, 1476
MunI CAATTG 1 cut(s) 1732
Mva1269I GAATGC 1 cut(s) 1519
MvaI CCWGG 1 cut(s) 715
MvnI CGCG 5 cut(s) 294, 492, 1334, 1563, 1681
MwoI GCNNNNNNNGC 5 cut(s) 300, 977, 1326, 1680, 1689
NaeI GCCGGC 1 cut(s) 1170
NciI CCSGG 3 cut(s) 53, 866, 1476
NgoMIV GCCGGC 1 cut(s) 1168
NlaIII CATG 5 cut(s) 469, 1145, 1547, 1613, 1752
NlaIV GGNNCC 6 cut(s) 272, 561, 630, 912, 960, 1445
NmeAIII GCCGAG 3 cut(s) 90, 321, 400
NmuCI GTSAC 4 cut(s) 83, 877, 1504, 1513
NruI TCGCGA 1 cut(s) 1334
NspI RCATGY 1 cut(s) 1145
NspV TTCGAA 1 cut(s) 1005
OliI CACNNNNGTG 1 cut(s) 1511
PaeI GCATGC 1 cut(s) 1145
PaeR7I CTCGAG 3 cut(s) 284, 473, 755
PagI TCATGA 1 cut(s) 1609
PciSI GCTCTTC 1 cut(s) 1529
PctI GAATGC 1 cut(s) 1519
PdiI GCCGGC 1 cut(s) 1170
PfeI GAWTC 6 cut(s) 393, 687, 726, 802, 811, 1580
PkrI GCNGC 6 cut(s) 331, 347, 1562, 1641, 1673, 1706
PleI GAGTC 1 cut(s) 1006
PpsI GAGTC 1 cut(s) 1006
Psp124BI GAGCTC 5 cut(s) 111, 357, 762, 1274, 1322
Psp6I CCWGG 1 cut(s) 713
PspGI CCWGG 1 cut(s) 713
PspN4I GGNNCC 6 cut(s) 272, 561, 630, 912, 960, 1445
PspPI GGNCC 3 cut(s) 55, 194, 270
PspXI VCTCGAGB 2 cut(s) 284, 755
PsrI GAACNNNNNNTAC 2 cut(s) 1331, 1363
PstI CTGCAG 1 cut(s) 1633
PvuII CAGCTG 1 cut(s) 1180
RruI TCGCGA 1 cut(s) 1334
RsaI GTAC 9 cut(s) 5, 317, 364, 428, 753, 797, 818, 1307, 1445
RsaNI GTAC 9 cut(s) 4, 316, 363, 427, 752, 796, 817, 1306, 1444
RseI CAYNNNNRTG 3 cut(s) 467, 1289, 1511
Rsr2I CGGWCCG 1 cut(s) 55
RsrII CGGWCCG 1 cut(s) 55
SacI GAGCTC 5 cut(s) 111, 357, 762, 1274, 1322
SalI GTCGAC 1 cut(s) 884
SapI GCTCTTC 1 cut(s) 1529
SaqAI TTAA 2 cut(s) 440, 1566
SatI GCNGC 6 cut(s) 330, 346, 1561, 1640, 1672, 1705
Sau96I GGNCC 3 cut(s) 55, 194, 270
ScaI AGTACT 1 cut(s) 428
SchI GAGTC 1 cut(s) 1006
ScrFI CCNGG 4 cut(s) 53, 715, 866, 1476
SduI GDGCHC 5 cut(s) 111, 357, 762, 1274, 1322
SfaNI GCATC 2 cut(s) 333, 459
SfcI CTRYAG 1 cut(s) 1629
Sfr274I CTCGAG 3 cut(s) 284, 473, 755
SfuI TTCGAA 1 cut(s) 1005
SgrAI CRCCGGYG 2 cut(s) 500, 932
SgrDI CGTCGACG 1 cut(s) 884
SinI GGWCC 2 cut(s) 55, 270
SlaI CTCGAG 3 cut(s) 284, 473, 755
SmiMI CAYNNNNRTG 3 cut(s) 467, 1289, 1511
SmlI CTYRAG 3 cut(s) 284, 473, 755
SmoI CTYRAG 3 cut(s) 284, 473, 755
SphI GCATGC 1 cut(s) 1145
Sse9I AATT 7 cut(s) 607, 832, 1007, 1077, 1202, 1720, 1732
SspMI CTAG 2 cut(s) 564, 1428
SstI GAGCTC 5 cut(s) 111, 357, 762, 1274, 1322
StyD4I CCNGG 4 cut(s) 51, 713, 864, 1474
StyI CCWWGG 2 cut(s) 266, 563
TaaI ACNGT 2 cut(s) 883, 1138
TaiI ACGT 7 cut(s) 162, 279, 483, 516, 549, 748, 768
TaqII GACCGA 1 cut(s) 684
TasI AATT 7 cut(s) 607, 832, 1007, 1077, 1202, 1720, 1732
TatI WGTACW 3 cut(s) 426, 816, 1305
TauI GCSGC 3 cut(s) 332, 348, 1563
TfiI GAWTC 6 cut(s) 393, 687, 726, 802, 811, 1580
Tru1I TTAA 2 cut(s) 440, 1566
Tru9I TTAA 2 cut(s) 440, 1566
TseFI GTSAC 4 cut(s) 83, 877, 1504, 1513
TseI GCWGC 3 cut(s) 1639, 1671, 1704
Tsp45I GTSAC 4 cut(s) 83, 877, 1504, 1513
TspDTI ATGAA 2 cut(s) 1071, 1541
TspGWI ACGGA 4 cut(s) 71, 350, 870, 1227
VpaK11BI GGWCC 2 cut(s) 55, 270
XapI RAATTY 2 cut(s) 607, 1077
XceI RCATGY 1 cut(s) 1145
XhoI CTCGAG 3 cut(s) 284, 473, 755
XmaJI CCTAGG 1 cut(s) 563
XmiI GTMKAC 3 cut(s) 507, 885, 1742
XspI CTAG 2 cut(s) 564, 1428
ZrmI AGTACT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.