AT5G08560

C-terminal to LisH motif.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004773
Physical Location & Seq
Reverse (-)
2770782 .. 2774714
3933 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G08560.3

Sequence Viewer

Length: 1770 bp
ATGGGAGTTGTGGAGGATACTGAACCTCCTTTGAAACGTGCTAAGAGGCTTGCTGACGAACCGAACGGCTTCTCCGCGAACTCGTCTGTTAGAGGTAGTAGTGTGAACAGCAACTCTTTGGGAGACCTGATGGCAAGGCCTCTGCCCTCCCAAGGGGATGACGAAACTATCGGCTCCAAAGGGGTCATTAGAAAATCTGAATTCGTTAGGATTATCACTAGGGCTCTTTATTCTCTTGGATATGATAAAACCGGGGCTATGTTGGAGGAAGAATCCGGTATCTCTCTGCACAATTCCACCATTAAGCTGTTTCTGCAGCAGGTTAAGGACGGCAAATGGGATCAAAGTGTTAAAACATTGCACAGAATCGGTTTCCCTGATGAGAAGGCTGTCAAGGCTGCGTCTTTCTTGCTCCTAGAGCAAAAGTTCTTGGAGTTTCTCAAAGTTGAAAAGATAGCTGATGCGCTACGCACCTTAAGGAATGAGATGGCGCCTCTTCGTATTAATACAAAGCGTGTTCATGAGCTCGCTTCCTCCCTTATATCACCTTCAAGCTTTATATCACATACCACTTCGACTCCAGGTAAAGAAAGTGTGAATTCAAGGTCTAAGGTTTTGGAGGAACTGCAAACCTTGCTTCCTGCTTCTGTTATAATCCCAGAAAAGAGGTTGGAGTGTTTAGTTGAGAATTCACTTCATATTCAGCGGGATTCTTGTGTTTTCCATAATACTTTGGATAGTGATTTGTCTTTGTATTCTGATCATCAATGCGGGAAGCACCAAATTCCTTCTCAGACTGCTCAGATCTTGGAGTCACATACCGATGAAGTTTGGTTCTTGCAATTTTCACATAATGGCAAATATTTGGCTTCATCTTCCAAGGATCAAACCGCAATTATATGGGAGATCAGCGCAGATGGGCACATTTCATTGAAGCATACACTTGTTGGCCACCACAAACCCGTGATTGCCATCTTATGGAGTCCTGATGATCGTCAAGTTCTTACATGTGGAGCAGAAGAGGTTATCAGACGCTGGGATGTTGATTCAGGAGACTGTGTTCACATGTATGAAAAAGGCGGTATCAGTCCCATTTCTTGCGGATGGTATCCTGATGGGCAGGGGATAATCGCCGGGATGACAGACCGAAGCATCTGCATGTGGGATTTAGATGGTAGAGAGAAGGAATGCTGGAAAGGTCAGAGGACTCAAAAGGTATCAGATATAGCAATGACGGATGATGGAAAGTGGCTCGTAAGTGTATGCAAAGATTCCGTGATATCACTATTTGATAGAGAAGCTACAGTTGAGAGATTAATAGAAGAGGAAGACATGATTACCTCGTTCTCGCTTTCGAATGACAACAAATATATCCTGGTAAACCTCCTCAACCAGGAAATCCGTCTCTGGAATATTGAAGGTGACCCCAAGATTGTGTCGAGATACAAAGGCCACAAGCGTTCACGATTCATCATCAGATCATGCTTTGGTGGGTATAAACAAGCTTTCATCGCTAGCGGAAGCGAGGACTCTCAGGTATATATATGGCACAGATCTACAGGGAAGTTAATAGTTGAGCTACCAGGCCATGCAGGAGCAGTTAACTGCGTGAGCTGGAGCCCGACGAATCTACACATGTTGGCTTCAGCAAGCGATGATGGAACAATAAGGATATGGGGACTTGACCGGATTAACCAACAAAACCAGAAGAAGAAGCTAGTGCAAGGAAGTAGCAGTAATGGTGTGATTCACCGATGCAATGGGAATTGA

Protein Analysis

589

Amino Acids

65.83

Weight (kDa)

6.48

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_WDR26 PF23627 55 - 97 2.4e-21 WDR26 Lis-homology motif
Beta-prop_RIG_2nd PF23775 226 - 371 1.1e-06 RIG second beta-propeller
Beta-prop_Aladin PF25460 263 - 350 1.3e-08 Aladin seven-bladed propeller
WD40_CDC20-Fz PF24807 264 - 429 6.3e-18 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 264 - 480 6e-19 Prp19 WD40 domain
Beta-prop_TEP1_2nd PF25047 265 - 357 2.2e-08 TEP-1 second beta-propeller
Beta-prop_WDR3_1st PF25173 265 - 390 6.6e-19 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 266 - 362 1.3e-09 CAF1B/HIR1 beta-propeller domain
Beta-prop_EML PF23409 266 - 434 1.8e-08 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_IFT122_1st PF23381 267 - 395 2.2e-07 IFT122 first beta-propeller
WD40 PF00400 268 - 301 2.9e-07 WD domain, G-beta repeat
WD40_MABP1-WDR62_2nd PF24782 268 - 458 3.1e-09 MABP1/WDR62 second WD40 domain
EIF3I PF24805 270 - 358 2e-06 EIF3I
Beta-prop_WDR5 PF25175 270 - 559 2.9e-57 WDR5 beta-propeller domain
WD40_WDHD1_1st PF24817 272 - 338 2.1e-08 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 273 - 527 5.7e-37 THOC3 beta-propeller domain
WD40_Gbeta PF25391 279 - 516 6.6e-11 G protein beta WD-40 repeat protein
WDR55 PF24796 312 - 562 3.5e-18 WDR55
Beta-prop_WDR3_2nd PF25172 313 - 432 1.3e-10 WDR3 second beta-propeller domain
WD40_WDHD1_1st PF24817 318 - 484 7.2e-12 WDHD1 first WD40 domain
Beta-prop_EML_2 PF23414 327 - 474 6.3e-16 Echinoderm microtubule-associated protein second beta-propeller
EIF3I PF24805 366 - 458 3.3e-06 EIF3I
Beta-prop_WDR3_1st PF25173 369 - 472 6.9e-11 WDR3 first beta-propeller domain
Beta-prop_WDR36-Utp21_1st PF25171 382 - 571 2.1e-07 WDR36/Utp21 first beta-propeller
Beta-prop_EML PF23409 405 - 573 2.1e-06 Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_WDR19_1st PF23389 413 - 559 4.8e-06 WDR19 first beta-propeller
Beta-prop_WDR3_2nd PF25172 437 - 565 2.1e-08 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 437 - 563 1.4e-16 WDR3 first beta-propeller domain
WD40_MABP1-WDR62_2nd PF24782 446 - 561 4.6e-06 MABP1/WDR62 second WD40 domain
Beta-prop_EML_2 PF23414 465 - 578 5.1e-07 Echinoderm microtubule-associated protein second beta-propeller
WD40_Prp19 PF24814 500 - 564 4.1e-11 Prp19 WD40 domain
Beta-prop_HPS5 PF23756 500 - 575 6.9e-07 HPS5 beta-propeller
Beta-prop_TEP1_2nd PF25047 501 - 577 3.5e-06 TEP-1 second beta-propeller
WD40_WDHD1_1st PF24817 501 - 582 1.3e-09 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 501 - 563 3.7e-10 CDC20/Fizzy WD40 domain
Beta-prop_CAF1B_HIR1 PF24105 503 - 567 6.4e-08 CAF1B/HIR1 beta-propeller domain
Beta-prop_THOC3 PF25174 503 - 567 1.9e-14 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 515 - 563 7e-09 WDR5 beta-propeller domain
WD40 PF00400 521 - 559 3.8e-10 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 653
Acc36I ACCTGC 1 cut(s) 310
AccB1I GGYRCC 1 cut(s) 490
AccB7I CCANNNNNTGG 1 cut(s) 978
AccII CGCG 1 cut(s) 77
AciI CCGC 7 cut(s) 75, 706, 771, 891, 1080, 1101, 1518
AclWI GGATC 2 cut(s) 348, 891
AcoI YGGCCR 1 cut(s) 949
AcsI RAATTY 4 cut(s) 200, 598, 688, 783
AcuI CTGAAG 1 cut(s) 1629
AcyI GRCGYC 1 cut(s) 491
AfiI CCNNNNNNNGG 4 cut(s) 152, 153, 978, 1393
AflII CTTAAG 1 cut(s) 475
AflIII ACRYGT 3 cut(s) 1007, 1065, 1635
AgsI TTSAA 6 cut(s) 34, 449, 552, 603, 934, 1418
AjnI CCWGG 4 cut(s) 580, 1374, 1392, 1582
AloI GAACNNNNNNTCC 4 cut(s) 56, 88, 1044, 1076
AluBI AGCT 9 cut(s) 307, 458, 526, 555, 1301, 1505, 1579, 1614, 1717
AluI AGCT 9 cut(s) 307, 458, 526, 555, 1301, 1505, 1579, 1614, 1717
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 3 cut(s) 117, 1047, 1409
AlwI GGATC 2 cut(s) 348, 891
AlwNI CAGNNNCTG 1 cut(s) 1035
AoxI GGCC 4 cut(s) 137, 949, 1450, 1585
ApeKI GCWGC 2 cut(s) 316, 398
ApoI RAATTY 4 cut(s) 200, 598, 688, 783
AseI ATTAAT 2 cut(s) 504, 1316
Asp700I GAANNNNTTC 1 cut(s) 68
AspLEI GCGC 3 cut(s) 466, 493, 914
AsuC2I CCSGG 2 cut(s) 253, 1135
AsuHPI GGTGA 3 cut(s) 537, 1433, 1742
AsuII TTCGAA 1 cut(s) 1355
AsuNHI GCTAGC 1 cut(s) 1514
BaeGI GKGCMC 1 cut(s) 924
BalI TGGCCA 1 cut(s) 951
BanI GGYRCC 1 cut(s) 490
BanII GRGCYC 3 cut(s) 226, 528, 1622
BbsI GAAGAC 1 cut(s) 1335
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 2 cut(s) 328, 385
BccI CCATC 9 cut(s) 124, 481, 911, 980, 1098, 1109, 1166, 1235, 1652
BceAI ACGGC 2 cut(s) 82, 346
BcgI CGANNNNNNTGC 2 cut(s) 1137, 1171
BciT130I CCWGG 4 cut(s) 582, 1376, 1394, 1584
BciVI GTATCC 2 cut(s) 10, 1119
BclI TGATCA 1 cut(s) 760
BcnI CCSGG 2 cut(s) 253, 1135
BcoDI GTCTC 3 cut(s) 117, 1047, 1409
BfaI CTAG 4 cut(s) 219, 416, 1515, 1718
BfmI CTRYAG 3 cut(s) 314, 1302, 1557
BfoI RGCGCY 1 cut(s) 494
BfrI CTTAAG 1 cut(s) 475
BfuAI ACCTGC 1 cut(s) 310
BfuI GTATCC 2 cut(s) 10, 1119
BglII AGATCT 2 cut(s) 804, 1553
BisI GCNGC 2 cut(s) 317, 399
BlsI GCNGC 2 cut(s) 318, 400
Bme1390I CCNGG 6 cut(s) 253, 582, 1135, 1376, 1394, 1584
BmiI GGNNCC 3 cut(s) 175, 492, 1619
BmrFI CCNGG 6 cut(s) 253, 582, 1135, 1376, 1394, 1584
BmsI GCATC 3 cut(s) 451, 1161, 1745
BmtI GCTAGC 1 cut(s) 1518
BpiI GAAGAC 1 cut(s) 1335
BpmI CTGGAG 2 cut(s) 564, 1636
Bpu14I TTCGAA 1 cut(s) 1355
BpuMI CCSGG 2 cut(s) 253, 1135
BsaBI GATNNNNATC 1 cut(s) 971
BsaHI GRCGYC 1 cut(s) 491
BsaI GGTCTC 1 cut(s) 117
BsaJI CCNNGG 3 cut(s) 151, 252, 879
BsaWI WCCGGW 2 cut(s) 275, 1686
BsaXI ACNNNNNCTCC 8 cut(s) 10, 40, 56, 86, 562, 592, 1044, 1074
Bsc4I CCNNNNNNNGG 4 cut(s) 152, 153, 978, 1393
Bse3DI GCAATG 3 cut(s) 356, 1236, 1765
Bse8I GATNNNNATC 1 cut(s) 971
BseBI CCWGG 4 cut(s) 582, 1376, 1394, 1584
BseDI CCNNGG 3 cut(s) 151, 252, 879
BseGI GGATG 5 cut(s) 163, 1045, 1109, 1143, 1243
BseJI GATNNNNATC 1 cut(s) 971
BseLI CCNNNNNNNGG 4 cut(s) 152, 153, 978, 1393
BseMI GCAATG 3 cut(s) 356, 1236, 1765
BseMII CTCAG 3 cut(s) 806, 815, 1547
BseRI GAGGAG 1 cut(s) 1376
BseSI GKGCMC 1 cut(s) 924
BseXI GCAGC 2 cut(s) 328, 385
BseYI CCCAGC 1 cut(s) 1035
BsgI GTGCAG 1 cut(s) 272
Bsh1236I CGCG 1 cut(s) 77
BshFI GGCC 4 cut(s) 139, 951, 1452, 1587
BshNI GGYRCC 1 cut(s) 490
BsiHKAI GWGCWC 1 cut(s) 528
BsiSI CCGG 4 cut(s) 252, 276, 1134, 1687
BslFI GGGAC 2 cut(s) 1074, 1692
BslI CCNNNNNNNGG 4 cut(s) 152, 153, 978, 1393
BsmAI GTCTC 3 cut(s) 117, 1047, 1409
BsmBI CGTCTC 1 cut(s) 1409
BsmFI GGGAC 2 cut(s) 1074, 1692
BsmI GAATGC 1 cut(s) 1193
BsnI GGCC 4 cut(s) 139, 951, 1452, 1587
Bso31I GGTCTC 1 cut(s) 117
Bsp119I TTCGAA 1 cut(s) 1355
Bsp1286I GDGCHC 4 cut(s) 226, 528, 924, 1622
Bsp143I GATC 8 cut(s) 340, 760, 804, 883, 906, 991, 1478, 1553
BspACI CCGC 7 cut(s) 75, 706, 771, 891, 1080, 1101, 1518
BspANI GGCC 4 cut(s) 139, 951, 1452, 1587
BspCNI CTCAG 3 cut(s) 805, 814, 1546
BspFNI CGCG 1 cut(s) 77
BspHI TCATGA 1 cut(s) 520
BspLI GGNNCC 3 cut(s) 175, 492, 1619
BspMAI CTGCAG 1 cut(s) 318
BspMI ACCTGC 1 cut(s) 310
BspOI GCTAGC 1 cut(s) 1518
BspPI GGATC 2 cut(s) 348, 891
BspT104I TTCGAA 1 cut(s) 1355
BspT107I GGYRCC 1 cut(s) 490
BspTI CTTAAG 1 cut(s) 475
BspTNI GGTCTC 1 cut(s) 117
BsrDI GCAATG 3 cut(s) 356, 1236, 1765
BssECI CCNNGG 3 cut(s) 151, 252, 879
BssMI GATC 8 cut(s) 340, 760, 804, 883, 906, 991, 1478, 1553
BssNI GRCGYC 1 cut(s) 491
BssT1I CCWWGG 2 cut(s) 151, 879
Bst2UI CCWGG 4 cut(s) 582, 1376, 1394, 1584
Bst4CI ACNGT 2 cut(s) 1058, 1306
Bst6I CTCTTC 3 cut(s) 501, 1014, 1317
BstACI GRCGYC 1 cut(s) 491
BstAFI CTTAAG 1 cut(s) 475
BstAPI GCANNNNNTGC 1 cut(s) 634
BstBI TTCGAA 1 cut(s) 1355
BstC8I GCNNGC 4 cut(s) 51, 528, 1516, 1651
BstDEI CTNAG 5 cut(s) 42, 609, 792, 801, 1533
BstEII GGTNACC 1 cut(s) 1421
BstENI CCTNNNNNAGG 1 cut(s) 1391
BstF5I GGATG 5 cut(s) 163, 1045, 1109, 1143, 1243
BstFNI CGCG 1 cut(s) 77
BstH2I RGCGCY 1 cut(s) 494
BstHHI GCGC 3 cut(s) 466, 493, 914
BstKTI GATC 8 cut(s) 343, 763, 807, 886, 909, 994, 1481, 1556
BstMAI GTCTC 3 cut(s) 117, 1047, 1409
BstMBI GATC 8 cut(s) 340, 760, 804, 883, 906, 991, 1478, 1553
BstMWI GCNNNNNNNGC 5 cut(s) 313, 395, 418, 634, 1511
BstNI CCWGG 4 cut(s) 582, 1376, 1394, 1584
BstNSI RCATGY 4 cut(s) 1011, 1069, 1162, 1639
BstPI GGTNACC 1 cut(s) 1421
BstSCI CCNGG 6 cut(s) 251, 580, 1133, 1374, 1392, 1582
BstSFI CTRYAG 3 cut(s) 314, 1302, 1557
BstSLI GKGCMC 1 cut(s) 924
BstUI CGCG 1 cut(s) 77
BstV1I GCAGC 2 cut(s) 328, 385
BstV2I GAAGAC 1 cut(s) 1335
BstX2I RGATCY 2 cut(s) 804, 1553
BstYI RGATCY 2 cut(s) 804, 1553
BsuI GTATCC 2 cut(s) 10, 1119
BsuRI GGCC 4 cut(s) 139, 951, 1452, 1587
BtgZI GCGATG 2 cut(s) 1495, 1668
BtsCI GGATG 5 cut(s) 163, 1045, 1109, 1143, 1243
BveI ACCTGC 1 cut(s) 310
Cac8I GCNNGC 4 cut(s) 51, 528, 1516, 1651
CaiI CAGNNNCTG 1 cut(s) 1035
CciI TCATGA 1 cut(s) 520
CfoI GCGC 3 cut(s) 466, 493, 914
CseI GACGC 2 cut(s) 390, 1041
CviAII CATG 8 cut(s) 521, 1008, 1066, 1159, 1333, 1482, 1589, 1636
DdeI CTNAG 5 cut(s) 42, 609, 792, 801, 1533
DinI GGCGCC 1 cut(s) 492
DpnI GATC 8 cut(s) 342, 762, 806, 885, 908, 993, 1480, 1555
DpnII GATC 8 cut(s) 340, 760, 804, 883, 906, 991, 1478, 1553
EaeI YGGCCR 1 cut(s) 949
Eam1104I CTCTTC 3 cut(s) 501, 1014, 1317
EarI CTCTTC 3 cut(s) 501, 1014, 1317
Ecl136II GAGCTC 1 cut(s) 526
Eco130I CCWWGG 2 cut(s) 151, 879
Eco147I AGGCCT 1 cut(s) 139
Eco24I GRGCYC 3 cut(s) 226, 528, 1622
Eco31I GGTCTC 1 cut(s) 117
Eco32I GATATC 1 cut(s) 1281
Eco53kI GAGCTC 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 1629
Eco91I GGTNACC 1 cut(s) 1421
EcoICRI GAGCTC 1 cut(s) 526
EcoNI CCTNNNNNAGG 1 cut(s) 1391
EcoO65I GGTNACC 1 cut(s) 1421
EcoRI GAATTC 3 cut(s) 200, 598, 688
EcoRII CCWGG 4 cut(s) 580, 1374, 1392, 1582
EcoRV GATATC 1 cut(s) 1281
EcoT14I CCWWGG 2 cut(s) 151, 879
EcoT38I GRGCYC 3 cut(s) 226, 528, 1622
EgeI GGCGCC 1 cut(s) 492
EheI GGCGCC 1 cut(s) 492
ErhI CCWWGG 2 cut(s) 151, 879
Esp3I CGTCTC 1 cut(s) 1409
FaeI CATG 8 cut(s) 524, 1011, 1069, 1162, 1336, 1485, 1592, 1639
FaqI GGGAC 2 cut(s) 1074, 1692
FatI CATG 8 cut(s) 520, 1007, 1065, 1158, 1332, 1481, 1588, 1635
FauI CCCGC 2 cut(s) 699, 764
FbaI TGATCA 1 cut(s) 760
Fnu4HI GCNGC 2 cut(s) 317, 399
FokI GGATG 5 cut(s) 170, 1052, 1116, 1150, 1250
FriOI GRGCYC 3 cut(s) 226, 528, 1622
Fsp4HI GCNGC 2 cut(s) 317, 399
FspBI CTAG 4 cut(s) 219, 416, 1515, 1718
GlaI GCGC 3 cut(s) 465, 492, 913
GluI GCNGC 2 cut(s) 317, 399
GsaI CCCAGC 1 cut(s) 1039
GsuI CTGGAG 2 cut(s) 564, 1636
HaeII RGCGCY 1 cut(s) 494
HaeIII GGCC 4 cut(s) 139, 951, 1452, 1587
HapII CCGG 4 cut(s) 252, 276, 1134, 1687
HgaI GACGC 2 cut(s) 390, 1041
HhaI GCGC 3 cut(s) 466, 493, 914
Hin1I GRCGYC 1 cut(s) 491
Hin1II CATG 8 cut(s) 524, 1011, 1069, 1162, 1336, 1485, 1592, 1639
Hin6I GCGC 3 cut(s) 464, 491, 912
HinP1I GCGC 3 cut(s) 464, 491, 912
HincII GTYRAC 1 cut(s) 1603
HindII GTYRAC 1 cut(s) 1603
HindIII AAGCTT 2 cut(s) 553, 1503
HpaI GTTAAC 1 cut(s) 1603
HpaII CCGG 4 cut(s) 252, 276, 1134, 1687
HphI GGTGA 3 cut(s) 537, 1433, 1742
Hpy166II GTNNAC 5 cut(s) 106, 1063, 1381, 1463, 1603
Hpy188I TCNGA 8 cut(s) 199, 760, 795, 804, 1031, 1203, 1222, 1478
Hpy188III TCNNGA 7 cut(s) 521, 986, 1050, 1112, 1408, 1440, 1464
Hpy8I GTNNAC 5 cut(s) 106, 1063, 1381, 1463, 1603
Hpy99I CGWCG 1 cut(s) 1627
HpyAV CCTTC 5 cut(s) 379, 558, 798, 1177, 1412
HpyCH4III ACNGT 2 cut(s) 1058, 1306
HpyCH4IV ACGT 1 cut(s) 37
HpyF10VI GCNNNNNNNGC 5 cut(s) 313, 395, 418, 634, 1511
HpyF3I CTNAG 5 cut(s) 42, 609, 792, 801, 1533
HpySE526I ACGT 1 cut(s) 37
Hsp92I GRCGYC 1 cut(s) 491
Hsp92II CATG 8 cut(s) 524, 1011, 1069, 1162, 1336, 1485, 1592, 1639
HspAI GCGC 3 cut(s) 464, 491, 912
KasI GGCGCC 1 cut(s) 490
Ksp22I TGATCA 1 cut(s) 760
KspAI GTTAAC 1 cut(s) 1603
Kzo9I GATC 8 cut(s) 340, 760, 804, 883, 906, 991, 1478, 1553
LmnI GCTCC 5 cut(s) 179, 417, 1013, 1595, 1617
Lsp1109I GCAGC 2 cut(s) 328, 385
LweI GCATC 3 cut(s) 451, 1161, 1745
MaeI CTAG 4 cut(s) 219, 416, 1515, 1718
MaeII ACGT 1 cut(s) 37
MaeIII GTNAC 2 cut(s) 813, 1421
MalI GATC 8 cut(s) 342, 762, 806, 885, 908, 993, 1480, 1555
MboI GATC 8 cut(s) 340, 760, 804, 883, 906, 991, 1478, 1553
MboII GAAGA 8 cut(s) 281, 488, 867, 1031, 1334, 1340, 1720, 1723
MflI RGATCY 2 cut(s) 804, 1553
MhlI GDGCHC 4 cut(s) 226, 528, 924, 1622
MlsI TGGCCA 1 cut(s) 951
MluCI AATT 8 cut(s) 200, 292, 598, 688, 783, 842, 894, 1765
MluNI TGGCCA 1 cut(s) 951
Mly113I GGCGCC 1 cut(s) 491
MlyI GAGTC 5 cut(s) 571, 821, 991, 1201, 1523
MmeI TCCRAC 2 cut(s) 243, 651
Mox20I TGGCCA 1 cut(s) 951
MroXI GAANNNNTTC 1 cut(s) 68
MscI TGGCCA 1 cut(s) 951
MseI TTAA 9 cut(s) 303, 324, 351, 476, 504, 1316, 1568, 1602, 1692
MslI CAYNNNNRTG 3 cut(s) 822, 1068, 1157
Msp20I TGGCCA 1 cut(s) 951
MspA1I CMGCKG 1 cut(s) 706
MspCI CTTAAG 1 cut(s) 475
MspI CCGG 4 cut(s) 252, 276, 1134, 1687
MspR9I CCNGG 6 cut(s) 253, 582, 1135, 1376, 1394, 1584
Mva1269I GAATGC 1 cut(s) 1193
MvaI CCWGG 4 cut(s) 582, 1376, 1394, 1584
MvnI CGCG 1 cut(s) 77
MwoI GCNNNNNNNGC 5 cut(s) 313, 395, 418, 634, 1511
NarI GGCGCC 1 cut(s) 491
NciI CCSGG 2 cut(s) 253, 1135
NdeII GATC 8 cut(s) 340, 760, 804, 883, 906, 991, 1478, 1553
NheI GCTAGC 1 cut(s) 1514
NlaIII CATG 8 cut(s) 524, 1011, 1069, 1162, 1336, 1485, 1592, 1639
NlaIV GGNNCC 3 cut(s) 175, 492, 1619
NmuCI GTSAC 2 cut(s) 813, 1421
NspI RCATGY 4 cut(s) 1011, 1069, 1162, 1639
NspV TTCGAA 1 cut(s) 1355
PagI TCATGA 1 cut(s) 520
PceI AGGCCT 1 cut(s) 139
PciI ACATGT 3 cut(s) 1007, 1065, 1635
PctI GAATGC 1 cut(s) 1193
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 8 cut(s) 272, 366, 710, 1046, 1271, 1467, 1627, 1747
PflMI CCANNNNNTGG 1 cut(s) 978
PkrI GCNGC 2 cut(s) 318, 400
PleI GAGTC 5 cut(s) 571, 820, 990, 1201, 1523
PluTI GGCGCC 1 cut(s) 494
PpsI GAGTC 5 cut(s) 571, 820, 990, 1201, 1523
PscI ACATGT 3 cut(s) 1007, 1065, 1635
PshBI ATTAAT 2 cut(s) 504, 1316
PsiI TTATAA 1 cut(s) 653
Psp124BI GAGCTC 1 cut(s) 528
Psp6I CCWGG 4 cut(s) 580, 1374, 1392, 1582
PspEI GGTNACC 1 cut(s) 1421
PspFI CCCAGC 1 cut(s) 1035
PspGI CCWGG 4 cut(s) 580, 1374, 1392, 1582
PspN4I GGNNCC 3 cut(s) 175, 492, 1619
PstI CTGCAG 1 cut(s) 318
PstNI CAGNNNCTG 1 cut(s) 1035
PsuI RGATCY 2 cut(s) 804, 1553
RseI CAYNNNNRTG 3 cut(s) 822, 1068, 1157
SacI GAGCTC 1 cut(s) 528
SaqAI TTAA 9 cut(s) 303, 324, 351, 476, 504, 1316, 1568, 1602, 1692
SatI GCNGC 2 cut(s) 317, 399
Sau3AI GATC 8 cut(s) 340, 760, 804, 883, 906, 991, 1478, 1553
SchI GAGTC 5 cut(s) 571, 821, 991, 1201, 1523
ScrFI CCNGG 6 cut(s) 253, 582, 1135, 1376, 1394, 1584
SduI GDGCHC 4 cut(s) 226, 528, 924, 1622
SfaNI GCATC 3 cut(s) 451, 1161, 1745
SfcI CTRYAG 3 cut(s) 314, 1302, 1557
SfoI GGCGCC 1 cut(s) 492
SfuI TTCGAA 1 cut(s) 1355
SmiMI CAYNNNNRTG 3 cut(s) 822, 1068, 1157
SmlI CTYRAG 1 cut(s) 475
SmoI CTYRAG 1 cut(s) 475
Sse9I AATT 8 cut(s) 200, 292, 598, 688, 783, 842, 894, 1765
SseBI AGGCCT 1 cut(s) 139
SsiI CCGC 7 cut(s) 75, 706, 771, 891, 1080, 1101, 1518
SspDI GGCGCC 1 cut(s) 490
SspI AATATT 2 cut(s) 863, 1414
SspMI CTAG 4 cut(s) 219, 416, 1515, 1718
SstI GAGCTC 1 cut(s) 528
StuI AGGCCT 1 cut(s) 139
StyD4I CCNGG 6 cut(s) 251, 580, 1133, 1374, 1392, 1582
StyI CCWWGG 2 cut(s) 151, 879
TaaI ACNGT 2 cut(s) 1058, 1306
TaiI ACGT 1 cut(s) 40
TaqI TCGA 3 cut(s) 575, 1355, 1439
TaqII GACCGA 1 cut(s) 1161
TasI AATT 8 cut(s) 200, 292, 598, 688, 783, 842, 894, 1765
TfiI GAWTC 8 cut(s) 272, 366, 710, 1046, 1271, 1467, 1627, 1747
Tru1I TTAA 9 cut(s) 303, 324, 351, 476, 504, 1316, 1568, 1602, 1692
Tru9I TTAA 9 cut(s) 303, 324, 351, 476, 504, 1316, 1568, 1602, 1692
TseFI GTSAC 2 cut(s) 813, 1421
TseI GCWGC 2 cut(s) 316, 398
Tsp45I GTSAC 2 cut(s) 813, 1421
TspDTI ATGAA 8 cut(s) 509, 686, 840, 861, 918, 1086, 1459, 1498
TspGWI ACGGA 3 cut(s) 1250, 1264, 1391
Van91I CCANNNNNTGG 1 cut(s) 978
Vha464I CTTAAG 1 cut(s) 475
VspI ATTAAT 2 cut(s) 504, 1316
XagI CCTNNNNNAGG 1 cut(s) 1391
XapI RAATTY 4 cut(s) 200, 598, 688, 783
XceI RCATGY 4 cut(s) 1011, 1069, 1162, 1639
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 4 cut(s) 219, 416, 1515, 1718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.