FvH4_6g38890

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0004773
Physical Location & Seq
Forward (+)
30767641 .. 30774549
6909 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38890.t2

Sequence Viewer

Length: 1737 bp
ATGGGAGGTGTGGGGGATGAAGAACCAGCCTCAAAACGCATGAAAGTATCCCCTGGAGACTTGAGAGGCCTTTCCAACGGTTCATCTTATACAGAGCCTGTAGCTGGCTCTTCAAGAGACTTGATGGCTCGGCCCCTAACATCCCAAGGGGACGAAGAGGTTGTTGGTTCAAAAGGTGTTATTAAGAGAGTTGAGTTTGTTCGACTAATAGCAAAGGCACTATATTCTCTTGGCTATAACAAAAGTGGTGCTCGTCTGGAGGAAGAGTCCGGAATACCTTTGCACTCTCCTGTGGTAAATCTATTTATGGAGCAAATTCTCAACGGAAATTGGGATGAAAGTGTTGTCACATTGCAGAAAATTGGTCTATCAGATGAAAATATCGCTAAATCTGCTTCCTTTCTGATATTGGAGCAGAAGTTTCTTGAACTTCTAGATGAGAAAAAGGACATGGATGCATTAAAGACATTGAGGACTGAGATTGCACCTCTTTGCATAAATGCTAGCAGAGTTCATGAGCTTTCATCGTGCATAGTTTCTCCTTCATTTGTTGGGTCCCCTGGTCAAAGCACTGTGAGGGCCAAGTCTCGGCCAAAGTTATTGGAGGAATTACAGAAGCTGCTCCCCCCAACTGTTATGATACTTGAAAGAAGGTTGGAACATTTAGTTGAACAAGCCCTCGTCTTGCAGCGTGATACTTGCATCTTTCACAACTCTTTGAACGAGGACATGTCTTTGTACACCGATCATCGGTGTGGGAGAGATCAGATTCCTTCTCGAACAGTGCAGGTATTACAAGACCACAGTGATGAAGTCTGGTTTGTTCAATTTTCACACAATGGGAAGTTCTTGGCTTCATCGTCAAGTGATAAATCAGCTATTATATGGGAGGTTGATGTGAATGGCTGGGTGTCGTTAAAGCATAGGCTAGTCGGTCACCATAAACCGGTTTGCTCTGTTTCATGGAGTCCTGATGACCGTCAGCTCCTCACTTGTGGAGTGGAGGAGGTTGTTAGGCGTTGGGATGTCTTTACTGGTGAATGCCTCCATGTTTATGAAAAACCTGGCCTTGGTCTATTGTCGTGTGGATGGTTTCCAGATGGGAAATGGATATTCTCTGGTGTCACTGACAAGAGTATCTGCACGTGGGAGTTAGAAGGGAAAGAGGTAGAGTGTTGGAAAGGGCAGCGAACTTCAAAAATCTCTGATTTGGAAATAACTAGTGATGGAAAGAAAATATTAAGTACTTGCAGAGAAAATGCTATATTGCTACTTGACAGGGAAGCCAAAGTTGACATACTACTTGAAGAGGATAAACCTATAACTTCTTTTTCTCTTTCAAGCGATGATAAGTTATTGTTGGTTAATCTTTTGAACCAAGAAATTCATCTGTGGAGCATAGCAGGTGAAGTCAAGCTTGTTGCCAAGTACCAAGGTCACAAACGCACCCGATTTGTCATCAGGTCTTGTTTTGGTGGACTTGAAGAAGCTTTCATTGCTAGTGGTAGTGAGGACTCACAGGTTTATGTATGGCATAGATGCTCAGGGGAGCTAATAGAAGTATTGCCAGGGCATTCCGGAGCTGTTAATTCTGTCAGCTGGAATCCAAAAAACCCACACATGTTGGCCTCAGCTAGTGATGACCGTACAATTCGAATATGGGGACTAAATGAGTTAAAAGTAAAACACAAGGATGCACACAGCAATGGCATCCATTGTTGCAATGGGGGAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

579

Amino Acids

64.36

Weight (kDa)

5.92

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_WDR26 PF23627 53 - 96 5.5e-22 WDR26 Lis-homology motif
WD40_Prp19 PF24814 259 - 388 1.2e-13 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 259 - 420 1.1e-12 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 260 - 389 3.7e-12 WDHD1 first WD40 domain
Beta-prop_IFT122_1st PF23381 261 - 389 8.1e-09 IFT122 first beta-propeller
WD40 PF00400 261 - 296 2.9e-07 WD domain, G-beta repeat
Beta-prop_WDR3_1st PF25173 261 - 384 2.3e-17 WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd PF25047 261 - 350 1.8e-08 TEP-1 second beta-propeller
Beta-prop_EML PF23409 261 - 428 6.2e-06 Echinoderm microtubule-associated protein first beta-propeller
WD40_Gbeta PF25391 265 - 386 1.9e-06 G protein beta WD-40 repeat protein
Beta-prop_WDR5 PF25175 265 - 527 1e-45 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 268 - 425 5.8e-26 THOC3 beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 302 - 391 3.8e-06 CAF1B/HIR1 beta-propeller domain
WD40_Gbeta PF25391 303 - 511 5.6e-07 G protein beta WD-40 repeat protein
WDR55 PF24796 310 - 558 4.1e-12 WDR55
Beta-prop_Aladin PF25460 313 - 423 8e-10 Aladin seven-bladed propeller
Beta-prop_EML_2 PF23414 320 - 478 3.2e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st PF25173 428 - 559 4.9e-16 WDR3 first beta-propeller domain
Beta-prop_WDR3_2nd PF25172 436 - 568 9.5e-08 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 494 - 565 2.9e-06 TEP-1 second beta-propeller
Beta-prop_CAF1B_HIR1 PF24105 495 - 565 1.1e-07 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 495 - 560 7.5e-07 EIF3I
WD40_WDHD1_1st PF24817 496 - 564 2.4e-07 WDHD1 first WD40 domain
WD40_CDC20-Fz PF24807 496 - 560 8.1e-11 CDC20/Fizzy WD40 domain
WD40_Prp19 PF24814 496 - 567 3.8e-11 Prp19 WD40 domain
Beta-prop_THOC3 PF25174 498 - 561 1.9e-14 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 510 - 575 5.9e-11 WDR5 beta-propeller domain
WD40 PF00400 516 - 554 9.5e-10 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1394
Acc36I ACCTGC 2 cut(s) 778, 1394
AccIII TCCGGA 2 cut(s) 269, 1577
AcoI YGGCCR 1 cut(s) 590
AcsI RAATTY 2 cut(s) 315, 1383
AcvI CACGTG 1 cut(s) 1146
AfaI GTAC 4 cut(s) 740, 1246, 1430, 1648
AfiI CCNNNNNNNGG 5 cut(s) 104, 588, 750, 946, 1070
AflIII ACRYGT 2 cut(s) 729, 1620
AgeI ACCGGT 1 cut(s) 946
AhlI ACTAGT 1 cut(s) 1220
AjnI CCWGG 4 cut(s) 52, 559, 1063, 1567
AjuI GAANNNNNNNTTGG 2 cut(s) 147, 179
Alw21I GWGCWC 1 cut(s) 253
Alw26I GTCTC 3 cut(s) 51, 111, 591
AlwNI CAGNNNCTG 2 cut(s) 98, 619
Aor13HI TCCGGA 2 cut(s) 269, 1577
AoxI GGCC 6 cut(s) 67, 131, 579, 590, 1066, 1626
ApeKI GCWGC 3 cut(s) 619, 688, 1186
ApoI RAATTY 2 cut(s) 315, 1383
AsiGI ACCGGT 1 cut(s) 946
AspS9I GGNCC 3 cut(s) 132, 555, 579
AsuHPI GGTGA 3 cut(s) 929, 1049, 1418
AsuII TTCGAA 1 cut(s) 1654
AsuNHI GCTAGC 1 cut(s) 503
AvaII GGWCC 1 cut(s) 555
BaeI ACNNNNGTAYC 2 cut(s) 1120, 1153
BbrPI CACGTG 1 cut(s) 1146
Bbv12I GWGCWC 1 cut(s) 253
BbvCI CCTCAGC 1 cut(s) 1630
BbvI GCAGC 3 cut(s) 606, 700, 1198
BccI CCATC 4 cut(s) 118, 1083, 1094, 1220
BciT130I CCWGG 4 cut(s) 54, 561, 1065, 1569
BciVI GTATCC 1 cut(s) 58
BcoDI GTCTC 3 cut(s) 51, 111, 591
BcuI ACTAGT 1 cut(s) 1220
BfaI CTAG 6 cut(s) 434, 504, 929, 1221, 1500, 1635
BfmI CTRYAG 1 cut(s) 99
BfuAI ACCTGC 2 cut(s) 778, 1394
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 3 cut(s) 620, 689, 1187
BlsI GCNGC 3 cut(s) 621, 690, 1188
BmcAI AGTACT 1 cut(s) 1246
Bme1390I CCNGG 4 cut(s) 54, 561, 1065, 1569
Bme18I GGWCC 1 cut(s) 555
BmgT120I GGNCC 3 cut(s) 132, 555, 579
BmiI GGNNCC 3 cut(s) 134, 556, 557
BmrFI CCNGG 4 cut(s) 54, 561, 1065, 1569
BmsI GCATC 5 cut(s) 445, 711, 1529, 1684, 1719
BmtI GCTAGC 1 cut(s) 507
BpmI CTGGAG 2 cut(s) 75, 278
Bpu10I CCTNAGC 2 cut(s) 1543, 1630
Bpu14I TTCGAA 1 cut(s) 1654
BpuEI CTTGAG 1 cut(s) 82
BsaAI YACGTR 1 cut(s) 1146
BsaJI CCNNGG 6 cut(s) 52, 145, 559, 1069, 1432, 1568
BsaWI WCCGGW 3 cut(s) 269, 946, 1577
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 404, 434
Bsc4I CCNNNNNNNGG 5 cut(s) 104, 588, 750, 946, 1070
Bse118I RCCGGY 1 cut(s) 946
Bse1I ACTGG 1 cut(s) 1039
Bse3DI GCAATG 4 cut(s) 350, 1494, 1711, 1729
BseAI TCCGGA 2 cut(s) 269, 1577
BseBI CCWGG 4 cut(s) 54, 561, 1065, 1569
BseDI CCNNGG 6 cut(s) 52, 145, 559, 1069, 1432, 1568
BseGI GGATG 8 cut(s) 22, 140, 340, 460, 1030, 1094, 1699, 1710
BseLI CCNNNNNNNGG 5 cut(s) 104, 588, 750, 946, 1070
BseMI GCAATG 4 cut(s) 350, 1494, 1711, 1729
BseMII CTCAG 3 cut(s) 468, 1557, 1644
BseNI ACTGG 1 cut(s) 1039
BseRI GAGGAG 2 cut(s) 977, 1019
BseXI GCAGC 3 cut(s) 606, 700, 1198
BseYI CCCAGC 1 cut(s) 906
BsgI GTGCAG 2 cut(s) 806, 1126
BshFI GGCC 6 cut(s) 69, 133, 581, 592, 1068, 1628
BshTI ACCGGT 1 cut(s) 946
BsiHKAI GWGCWC 1 cut(s) 253
BsiSI CCGG 3 cut(s) 270, 947, 1578
BslFI GGGAC 3 cut(s) 164, 541, 1677
BslI CCNNNNNNNGG 5 cut(s) 104, 588, 750, 946, 1070
BsmAI GTCTC 3 cut(s) 51, 111, 591
BsmFI GGGAC 3 cut(s) 164, 541, 1677
BsmI GAATGC 2 cut(s) 1046, 1573
BsnI GGCC 6 cut(s) 69, 133, 581, 592, 1068, 1628
Bsp119I TTCGAA 1 cut(s) 1654
Bsp1286I GDGCHC 1 cut(s) 253
Bsp13I TCCGGA 2 cut(s) 269, 1577
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 2 cut(s) 745, 763
BspANI GGCC 6 cut(s) 69, 133, 581, 592, 1068, 1628
BspCNI CTCAG 3 cut(s) 469, 1556, 1643
BspEI TCCGGA 2 cut(s) 269, 1577
BspHI TCATGA 1 cut(s) 514
BspLI GGNNCC 3 cut(s) 134, 556, 557
BspMI ACCTGC 2 cut(s) 778, 1394
BspOI GCTAGC 1 cut(s) 507
BspQI GCTCTTC 1 cut(s) 115
BspT104I TTCGAA 1 cut(s) 1654
BsrDI GCAATG 4 cut(s) 350, 1494, 1711, 1729
BsrFI RCCGGY 1 cut(s) 946
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 1 cut(s) 1039
BssAI RCCGGY 1 cut(s) 946
BssECI CCNNGG 6 cut(s) 52, 145, 559, 1069, 1432, 1568
BssMI GATC 2 cut(s) 745, 763
BssT1I CCWWGG 3 cut(s) 145, 1069, 1432
Bst2UI CCWGG 4 cut(s) 54, 561, 1065, 1569
Bst4CI ACNGT 7 cut(s) 80, 574, 634, 784, 806, 980, 1646
Bst6I CTCTTC 4 cut(s) 115, 150, 258, 1302
BstAUI TGTACA 1 cut(s) 738
BstBAI YACGTR 1 cut(s) 1146
BstBI TTCGAA 1 cut(s) 1654
BstC8I GCNNGC 2 cut(s) 106, 505
BstDEI CTNAG 3 cut(s) 477, 1543, 1630
BstEII GGTNACC 1 cut(s) 935
BstF5I GGATG 8 cut(s) 22, 140, 340, 460, 1030, 1094, 1699, 1710
BstKTI GATC 2 cut(s) 748, 766
BstMAI GTCTC 3 cut(s) 51, 111, 591
BstMBI GATC 2 cut(s) 745, 763
BstMWI GCNNNNNNNGC 2 cut(s) 392, 1496
BstNI CCWGG 4 cut(s) 54, 561, 1065, 1569
BstNSI RCATGY 2 cut(s) 733, 1624
BstPI GGTNACC 1 cut(s) 935
BstSCI CCNGG 4 cut(s) 52, 559, 1063, 1567
BstSFI CTRYAG 1 cut(s) 99
BstV1I GCAGC 3 cut(s) 606, 700, 1198
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 6 cut(s) 69, 133, 581, 592, 1068, 1628
BtgZI GCGATG 1 cut(s) 1359
BtsCI GGATG 8 cut(s) 22, 140, 340, 460, 1030, 1094, 1699, 1710
BtsIMutI CAGTG 4 cut(s) 570, 789, 811, 1125
BveI ACCTGC 2 cut(s) 778, 1394
Cac8I GCNNGC 2 cut(s) 106, 505
CaiI CAGNNNCTG 2 cut(s) 98, 619
CciI TCATGA 1 cut(s) 514
Cfr10I RCCGGY 1 cut(s) 946
Cfr13I GGNCC 3 cut(s) 132, 555, 579
Csp6I GTAC 4 cut(s) 739, 1245, 1429, 1647
CspAI ACCGGT 1 cut(s) 946
CspCI CAANNNNNGTGG 2 cut(s) 1605, 1640
CviAII CATG 7 cut(s) 40, 451, 515, 730, 963, 1049, 1621
CviQI GTAC 4 cut(s) 739, 1245, 1429, 1647
DdeI CTNAG 3 cut(s) 477, 1543, 1630
DpnI GATC 2 cut(s) 747, 765
DpnII GATC 2 cut(s) 745, 763
EaeI YGGCCR 1 cut(s) 590
Eam1104I CTCTTC 4 cut(s) 115, 150, 258, 1302
EarI CTCTTC 4 cut(s) 115, 150, 258, 1302
Eco130I CCWWGG 3 cut(s) 145, 1069, 1432
Eco147I AGGCCT 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 555
Eco72I CACGTG 1 cut(s) 1146
Eco91I GGTNACC 1 cut(s) 935
EcoO109I RGGNCCY 1 cut(s) 555
EcoO65I GGTNACC 1 cut(s) 935
EcoRII CCWGG 4 cut(s) 52, 559, 1063, 1567
EcoT14I CCWWGG 3 cut(s) 145, 1069, 1432
EcoT22I ATGCAT 1 cut(s) 460
ErhI CCWWGG 3 cut(s) 145, 1069, 1432
FaeI CATG 7 cut(s) 43, 454, 518, 733, 966, 1052, 1624
FalI AAGNNNNNCTT 2 cut(s) 1401, 1433
FaqI GGGAC 3 cut(s) 164, 541, 1677
FatI CATG 7 cut(s) 39, 450, 514, 729, 962, 1048, 1620
Fnu4HI GCNGC 3 cut(s) 620, 689, 1187
FokI GGATG 8 cut(s) 29, 127, 347, 467, 1037, 1101, 1697, 1706
Fsp4HI GCNGC 3 cut(s) 620, 689, 1187
FspBI CTAG 6 cut(s) 434, 504, 929, 1221, 1500, 1635
GluI GCNGC 3 cut(s) 620, 689, 1187
GsaI CCCAGC 1 cut(s) 910
GsuI CTGGAG 2 cut(s) 75, 278
HaeIII GGCC 6 cut(s) 69, 133, 581, 592, 1068, 1628
HapII CCGG 3 cut(s) 270, 947, 1578
Hin1II CATG 7 cut(s) 43, 454, 518, 733, 966, 1052, 1624
HincII GTYRAC 1 cut(s) 1294
HindII GTYRAC 1 cut(s) 1294
HindIII AAGCTT 2 cut(s) 1415, 1488
HinfI GANTC 5 cut(s) 266, 769, 967, 1514, 1603
HpaII CCGG 3 cut(s) 270, 947, 1578
HphI GGTGA 3 cut(s) 929, 1049, 1418
Hpy166II GTNNAC 3 cut(s) 741, 1294, 1478
Hpy188I TCNGA 4 cut(s) 373, 405, 768, 1207
Hpy8I GTNNAC 3 cut(s) 741, 1294, 1478
HpyAV CCTTC 4 cut(s) 552, 645, 783, 1151
HpyCH4III ACNGT 7 cut(s) 80, 574, 634, 784, 806, 980, 1646
HpyCH4IV ACGT 1 cut(s) 1145
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 1496
HpyF3I CTNAG 3 cut(s) 477, 1543, 1630
HpySE526I ACGT 1 cut(s) 1145
Hsp92II CATG 7 cut(s) 43, 454, 518, 733, 966, 1052, 1624
KflI GGGWCCC 1 cut(s) 555
Kpn2I TCCGGA 2 cut(s) 269, 1577
Kzo9I GATC 2 cut(s) 745, 763
LguI GCTCTTC 1 cut(s) 115
LmnI GCTCC 7 cut(s) 310, 412, 627, 990, 1395, 1549, 1580
Lsp1109I GCAGC 3 cut(s) 606, 700, 1198
LweI GCATC 5 cut(s) 445, 711, 1529, 1684, 1719
MaeI CTAG 6 cut(s) 434, 504, 929, 1221, 1500, 1635
MaeII ACGT 1 cut(s) 1145
MaeIII GTNAC 4 cut(s) 346, 935, 1123, 1436
MalI GATC 2 cut(s) 747, 765
MboI GATC 2 cut(s) 745, 763
MboII GAAGA 6 cut(s) 32, 102, 167, 275, 1319, 1496
MhlI GDGCHC 1 cut(s) 253
MluCI AATT 8 cut(s) 315, 328, 360, 608, 827, 1383, 1588, 1650
MlyI GAGTC 3 cut(s) 275, 976, 1508
MmeI TCCRAC 3 cut(s) 99, 636, 1157
Mph1103I ATGCAT 1 cut(s) 460
MroI TCCGGA 2 cut(s) 269, 1577
MseI TTAA 7 cut(s) 183, 461, 917, 1241, 1365, 1587, 1676
MslI CAYNNNNRTG 5 cut(s) 753, 807, 1053, 1692, 1704
MspA1I CMGCKG 1 cut(s) 1599
MspI CCGG 3 cut(s) 270, 947, 1578
MspR9I CCNGG 4 cut(s) 54, 561, 1065, 1569
Mva1269I GAATGC 2 cut(s) 1046, 1573
MvaI CCWGG 4 cut(s) 54, 561, 1065, 1569
MwoI GCNNNNNNNGC 2 cut(s) 392, 1496
NdeII GATC 2 cut(s) 745, 763
NheI GCTAGC 1 cut(s) 503
NlaIII CATG 7 cut(s) 43, 454, 518, 733, 966, 1052, 1624
NlaIV GGNNCC 3 cut(s) 134, 556, 557
NmeAIII GCCGAG 2 cut(s) 109, 568
NmuCI GTSAC 4 cut(s) 346, 935, 1123, 1436
NsiI ATGCAT 1 cut(s) 460
NspI RCATGY 2 cut(s) 733, 1624
NspV TTCGAA 1 cut(s) 1654
PagI TCATGA 1 cut(s) 514
PaqCI CACCTGC 1 cut(s) 1394
PceI AGGCCT 1 cut(s) 69
PciI ACATGT 2 cut(s) 729, 1620
PciSI GCTCTTC 1 cut(s) 115
PctI GAATGC 2 cut(s) 1046, 1573
PfeI GAWTC 2 cut(s) 769, 1603
PinAI ACCGGT 1 cut(s) 946
PkrI GCNGC 3 cut(s) 621, 690, 1188
PleI GAGTC 3 cut(s) 274, 975, 1508
PmaCI CACGTG 1 cut(s) 1146
PmlI CACGTG 1 cut(s) 1146
PpsI GAGTC 3 cut(s) 274, 975, 1508
Ppu21I YACGTR 1 cut(s) 1146
PpuMI RGGWCCY 1 cut(s) 555
PscI ACATGT 2 cut(s) 729, 1620
Psp5II RGGWCCY 1 cut(s) 555
Psp6I CCWGG 4 cut(s) 52, 559, 1063, 1567
PspCI CACGTG 1 cut(s) 1146
PspEI GGTNACC 1 cut(s) 935
PspFI CCCAGC 1 cut(s) 906
PspGI CCWGG 4 cut(s) 52, 559, 1063, 1567
PspN4I GGNNCC 3 cut(s) 134, 556, 557
PspPI GGNCC 3 cut(s) 132, 555, 579
PspPPI RGGWCCY 1 cut(s) 555
PstNI CAGNNNCTG 2 cut(s) 98, 619
PvuII CAGCTG 1 cut(s) 1599
RsaI GTAC 4 cut(s) 740, 1246, 1430, 1648
RsaNI GTAC 4 cut(s) 739, 1245, 1429, 1647
RseI CAYNNNNRTG 5 cut(s) 753, 807, 1053, 1692, 1704
SapI GCTCTTC 1 cut(s) 115
SaqAI TTAA 7 cut(s) 183, 461, 917, 1241, 1365, 1587, 1676
SatI GCNGC 3 cut(s) 620, 689, 1187
Sau3AI GATC 2 cut(s) 745, 763
Sau96I GGNCC 3 cut(s) 132, 555, 579
ScaI AGTACT 1 cut(s) 1246
SchI GAGTC 3 cut(s) 275, 976, 1508
ScrFI CCNGG 4 cut(s) 54, 561, 1065, 1569
SduI GDGCHC 1 cut(s) 253
SfaNI GCATC 5 cut(s) 445, 711, 1529, 1684, 1719
SfcI CTRYAG 1 cut(s) 99
SfuI TTCGAA 1 cut(s) 1654
SinI GGWCC 1 cut(s) 555
SmiMI CAYNNNNRTG 5 cut(s) 753, 807, 1053, 1692, 1704
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
SpeI ACTAGT 1 cut(s) 1220
Sse9I AATT 8 cut(s) 315, 328, 360, 608, 827, 1383, 1588, 1650
SseBI AGGCCT 1 cut(s) 69
SspI AATATT 1 cut(s) 1239
SspMI CTAG 6 cut(s) 434, 504, 929, 1221, 1500, 1635
StuI AGGCCT 1 cut(s) 69
StyD4I CCNGG 4 cut(s) 52, 559, 1063, 1567
StyI CCWWGG 3 cut(s) 145, 1069, 1432
TaaI ACNGT 7 cut(s) 80, 574, 634, 784, 806, 980, 1646
TaiI ACGT 1 cut(s) 1148
TaqI TCGA 3 cut(s) 202, 778, 1654
TaqII GACCGA 1 cut(s) 923
TasI AATT 8 cut(s) 315, 328, 360, 608, 827, 1383, 1588, 1650
TatI WGTACW 2 cut(s) 738, 1244
TfiI GAWTC 2 cut(s) 769, 1603
Tru1I TTAA 7 cut(s) 183, 461, 917, 1241, 1365, 1587, 1676
Tru9I TTAA 7 cut(s) 183, 461, 917, 1241, 1365, 1587, 1676
TscAI CASTG 4 cut(s) 577, 789, 811, 1132
TseFI GTSAC 4 cut(s) 346, 935, 1123, 1436
TseI GCWGC 3 cut(s) 619, 688, 1186
Tsp45I GTSAC 4 cut(s) 346, 935, 1123, 1436
TspGWI ACGGA 1 cut(s) 339
TspRI CASTG 4 cut(s) 577, 789, 811, 1132
VpaK11BI GGWCC 1 cut(s) 555
XapI RAATTY 2 cut(s) 315, 1383
XbaI TCTAGA 1 cut(s) 433
XceI RCATGY 2 cut(s) 733, 1624
XcmI CCANNNNNNNNNTGG 2 cut(s) 1104, 1721
XspI CTAG 6 cut(s) 434, 504, 929, 1221, 1500, 1635
ZrmI AGTACT 1 cut(s) 1246
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.