RLG00000025225

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0004773
Physical Location & Seq
Reverse (-)
43107189 .. 43108699
1511 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025225

Sequence Viewer

Length: 990 bp
ATGAGAGTTGAGTTTGTCCGACTAACAGCAAAGGCCCTATATATTCTCTTGGCTATAACAAAAGTACGTGGTGCTCCTCTGGAGGAAGAATCTGGAATACCCTTGCACTCTCCTGTGGTGAATCTATTTATGGAGCAAATTCTCAACGGCAATTGGGATGAAAGTGTCGTCACATTGCCCGGTCAAAACACTGTGAGGGCCAAGTCTCGGCCGAAGCAATTGGAGGAATTGCAAAAGCTGCTCCCACGAATTCCTTCTCAAACAGTGCAGGTTTGCTTTGTTTCATGGAGTCCTAATGACCGTCAGCTCCTCACTTGTGGAATGGAGGTGGTTGTCAGGCGTTGGGATGTCTCTACTGGTGAATGCCTCCATGTTTATGAAAAACCTGGCCTTGGTCTACTGTCATGTGGATGGTTTCCAGATCGGAAATGGATATTCTCTGGCGTCACTGAAAAGAGTAACTGCACGTGGGAGTTAGAAGGGAAAGAGCTGGAGTGTTGGAAAGTGCAGCGAACTTCAAAAATCTCTGATTTGGAAATAACTAGTGATGGAAAGAAAATATTAAGTACTTGTAGAGAAAATGTTATATTGCTACTTGACATGGAAGCCAAAGTTGACATCCTGCTTGGAGAAGATAAACCTATAACTTCGTTTTCTTTATCAATCGATGATAAGTTGTTCGTTAATCTTCTGAACCAAGAAATTCATCTCTGGAGCATAGCAGGTGATGTCAAGCTTGTTGCCAAGTACCAAGGTCACAAACGCACCCAATTTGTCATCAGGGGAATTCATAGTCATTGCCAGGACATTCTGGAGCTGTTAATCACACAGTTGGCCTCAGCTAGTGACGACCGAACAATTCGGATATGGGGACTAAATGAGATAAAAGTGAAACACAAGGATGCACACAGCAATGGCACATCCATTGTTGCAATGGGGGAAGTTGAAAAGGACTTGGAAAGAAAAGAGCTGGTTATAATTTCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

330

Amino Acids

37.29

Weight (kDa)

6.28

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_THOC3 PF25174 86 - 160 4.5e-06 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 87 - 264 4.7e-15 WDR5 beta-propeller domain
Beta-prop_Aladin PF25460 89 - 201 1.5e-07 Aladin seven-bladed propeller
WD40_Prp19 PF24814 94 - 299 4.1e-08 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 94 - 305 2.2e-06 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 96 - 242 1.5e-08 Echinoderm microtubule-associated protein second beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 977
AarI CACCTGC 1 cut(s) 713
Acc36I ACCTGC 2 cut(s) 259, 713
AccI GTMKAC 1 cut(s) 397
AcoI YGGCCR 1 cut(s) 209
AcsI RAATTY 4 cut(s) 138, 249, 702, 786
AcvI CACGTG 1 cut(s) 468
AcyI GRCGYC 1 cut(s) 444
AfaI GTAC 3 cut(s) 66, 568, 749
AfiI CCNNNNNNNGG 2 cut(s) 207, 392
AgsI TTSAA 2 cut(s) 519, 947
AhlI ACTAGT 1 cut(s) 542
AjnI CCWGG 2 cut(s) 385, 801
AluBI AGCT 7 cut(s) 238, 307, 490, 736, 817, 842, 970
AluI AGCT 7 cut(s) 238, 307, 490, 736, 817, 842, 970
Alw21I GWGCWC 1 cut(s) 76
Alw26I GTCTC 2 cut(s) 210, 355
AoxI GGCC 5 cut(s) 33, 198, 209, 388, 834
ApeKI GCWGC 2 cut(s) 238, 508
ApoI RAATTY 4 cut(s) 138, 249, 702, 786
Asp700I GAANNNNTTC 1 cut(s) 253
AspS9I GGNCC 2 cut(s) 34, 198
AsuC2I CCSGG 1 cut(s) 180
AsuHPI GGTGA 3 cut(s) 130, 371, 737
BbrPI CACGTG 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 76
BbvCI CCTCAGC 1 cut(s) 838
BbvI GCAGC 2 cut(s) 225, 520
BccI CCATC 2 cut(s) 405, 542
BceAI ACGGC 1 cut(s) 163
BciT130I CCWGG 2 cut(s) 387, 803
BcnI CCSGG 1 cut(s) 180
BcoDI GTCTC 2 cut(s) 210, 355
BcuI ACTAGT 1 cut(s) 542
BfaI CTAG 2 cut(s) 543, 843
BfuAI ACCTGC 2 cut(s) 259, 713
BisI GCNGC 2 cut(s) 239, 509
BlsI GCNGC 2 cut(s) 240, 510
BmcAI AGTACT 1 cut(s) 568
Bme1390I CCNGG 3 cut(s) 180, 387, 803
BmgT120I GGNCC 2 cut(s) 34, 198
BmrFI CCNGG 3 cut(s) 180, 387, 803
BmsI GCATC 1 cut(s) 892
BpmI CTGGAG 4 cut(s) 101, 512, 733, 833
Bpu10I CCTNAGC 1 cut(s) 838
BpuMI CCSGG 1 cut(s) 180
Bsa29I ATCGAT 1 cut(s) 666
BsaAI YACGTR 2 cut(s) 68, 468
BsaHI GRCGYC 1 cut(s) 444
BsaJI CCNNGG 2 cut(s) 391, 751
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 392
Bse1I ACTGG 1 cut(s) 361
Bse3DI GCAATG 4 cut(s) 173, 796, 919, 939
BseBI CCWGG 2 cut(s) 387, 803
BseCI ATCGAT 1 cut(s) 666
BseDI CCNNGG 2 cut(s) 391, 751
BseGI GGATG 6 cut(s) 163, 352, 416, 618, 907, 920
BseLI CCNNNNNNNGG 2 cut(s) 207, 392
BseMI GCAATG 4 cut(s) 173, 796, 919, 939
BseMII CTCAG 1 cut(s) 852
BseNI ACTGG 1 cut(s) 361
BseRI GAGGAG 2 cut(s) 66, 299
BseX3I CGGCCG 1 cut(s) 209
BseXI GCAGC 2 cut(s) 225, 520
BsgI GTGCAG 3 cut(s) 287, 448, 527
Bsh1285I CGRYCG 2 cut(s) 212, 853
BshFI GGCC 5 cut(s) 35, 200, 211, 390, 836
BshVI ATCGAT 1 cut(s) 666
BsiEI CGRYCG 2 cut(s) 212, 853
BsiHKAI GWGCWC 1 cut(s) 76
BsiSI CCGG 1 cut(s) 180
BslFI GGGAC 1 cut(s) 885
BslI CCNNNNNNNGG 2 cut(s) 207, 392
BsmAI GTCTC 2 cut(s) 210, 355
BsmFI GGGAC 1 cut(s) 885
BsmI GAATGC 1 cut(s) 368
BsnI GGCC 5 cut(s) 35, 200, 211, 390, 836
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 1 cut(s) 421
BspANI GGCC 5 cut(s) 35, 200, 211, 390, 836
BspCNI CTCAG 1 cut(s) 851
BspDI ATCGAT 1 cut(s) 666
BspMI ACCTGC 2 cut(s) 259, 713
BsrDI GCAATG 4 cut(s) 173, 796, 919, 939
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 2 cut(s) 391, 751
BssMI GATC 1 cut(s) 421
BssNI GRCGYC 1 cut(s) 444
BssT1I CCWWGG 2 cut(s) 391, 751
Bst2UI CCWGG 2 cut(s) 387, 803
Bst4CI ACNGT 5 cut(s) 193, 265, 302, 402, 831
BstACI GRCGYC 1 cut(s) 444
BstAPI GCANNNNNTGC 1 cut(s) 238
BstBAI YACGTR 2 cut(s) 68, 468
BstDEI CTNAG 1 cut(s) 838
BstF5I GGATG 6 cut(s) 163, 352, 416, 618, 907, 920
BstKTI GATC 1 cut(s) 424
BstMAI GTCTC 2 cut(s) 210, 355
BstMBI GATC 1 cut(s) 421
BstMCI CGRYCG 2 cut(s) 212, 853
BstMWI GCNNNNNNNGC 1 cut(s) 238
BstNI CCWGG 2 cut(s) 387, 803
BstSCI CCNGG 3 cut(s) 178, 385, 801
BstV1I GCAGC 2 cut(s) 225, 520
BstZI CGGCCG 1 cut(s) 209
Bsu15I ATCGAT 1 cut(s) 666
BsuRI GGCC 5 cut(s) 35, 200, 211, 390, 836
BsuTUI ATCGAT 1 cut(s) 666
BtsCI GGATG 6 cut(s) 163, 352, 416, 618, 907, 920
BtsIMutI CAGTG 3 cut(s) 189, 270, 447
BveI ACCTGC 2 cut(s) 259, 713
Cfr13I GGNCC 2 cut(s) 34, 198
ClaI ATCGAT 1 cut(s) 666
CseI GACGC 1 cut(s) 433
Csp6I GTAC 3 cut(s) 65, 567, 748
CviAII CATG 4 cut(s) 285, 371, 405, 601
CviQI GTAC 3 cut(s) 65, 567, 748
DdeI CTNAG 1 cut(s) 838
DpnI GATC 1 cut(s) 423
DpnII GATC 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 209
EagI CGGCCG 1 cut(s) 209
EclXI CGGCCG 1 cut(s) 209
Eco130I CCWWGG 2 cut(s) 391, 751
Eco52I CGGCCG 1 cut(s) 209
Eco72I CACGTG 1 cut(s) 468
EcoO109I RGGNCCY 1 cut(s) 34
EcoRI GAATTC 2 cut(s) 249, 786
EcoRII CCWGG 2 cut(s) 385, 801
EcoT14I CCWWGG 2 cut(s) 391, 751
ErhI CCWWGG 2 cut(s) 391, 751
FaeI CATG 4 cut(s) 288, 374, 408, 604
FaqI GGGAC 1 cut(s) 885
FatI CATG 4 cut(s) 284, 370, 404, 600
FblI GTMKAC 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 239, 509
FokI GGATG 6 cut(s) 170, 359, 423, 605, 907, 914
Fsp4HI GCNGC 2 cut(s) 239, 509
FspBI CTAG 2 cut(s) 543, 843
GluI GCNGC 2 cut(s) 239, 509
GsuI CTGGAG 4 cut(s) 101, 512, 733, 833
HaeIII GGCC 5 cut(s) 35, 200, 211, 390, 836
HapII CCGG 1 cut(s) 180
HgaI GACGC 1 cut(s) 433
Hin1I GRCGYC 1 cut(s) 444
Hin1II CATG 4 cut(s) 288, 374, 408, 604
HincII GTYRAC 1 cut(s) 616
HindII GTYRAC 1 cut(s) 616
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 3 cut(s) 89, 121, 289
HpaII CCGG 1 cut(s) 180
HphI GGTGA 3 cut(s) 130, 371, 737
Hpy166II GTNNAC 2 cut(s) 398, 616
Hpy188I TCNGA 6 cut(s) 20, 426, 529, 693, 864, 989
Hpy188III TCNNGA 5 cut(s) 80, 93, 419, 712, 812
Hpy8I GTNNAC 2 cut(s) 398, 616
HpyAV CCTTC 2 cut(s) 264, 473
HpyCH4III ACNGT 5 cut(s) 193, 265, 302, 402, 831
HpyCH4IV ACGT 2 cut(s) 67, 467
HpyCH4V TGCA 7 cut(s) 106, 232, 268, 465, 508, 905, 932
HpyF10VI GCNNNNNNNGC 1 cut(s) 238
HpyF3I CTNAG 1 cut(s) 838
HpySE526I ACGT 2 cut(s) 67, 467
Hsp92I GRCGYC 1 cut(s) 444
Hsp92II CATG 4 cut(s) 288, 374, 408, 604
Kzo9I GATC 1 cut(s) 421
LmnI GCTCC 6 cut(s) 79, 133, 246, 312, 714, 814
Lsp1109I GCAGC 2 cut(s) 225, 520
LweI GCATC 1 cut(s) 892
MaeI CTAG 2 cut(s) 543, 843
MaeII ACGT 2 cut(s) 67, 467
MaeIII GTNAC 5 cut(s) 169, 445, 458, 755, 845
MalI GATC 1 cut(s) 423
MboI GATC 1 cut(s) 421
MboII GAAGA 3 cut(s) 98, 644, 680
MfeI CAATTG 2 cut(s) 151, 218
MhlI GDGCHC 1 cut(s) 76
MlyI GAGTC 1 cut(s) 298
MmeI TCCRAC 2 cut(s) 43, 479
MnlI CCTC 8 cut(s) 76, 87, 189, 217, 319, 320, 377, 847
MroXI GAANNNNTTC 1 cut(s) 253
MseI TTAA 3 cut(s) 563, 684, 821
MslI CAYNNNNRTG 4 cut(s) 375, 409, 900, 912
MspI CCGG 1 cut(s) 180
MspR9I CCNGG 3 cut(s) 180, 387, 803
MunI CAATTG 2 cut(s) 151, 218
Mva1269I GAATGC 1 cut(s) 368
MvaI CCWGG 2 cut(s) 387, 803
MwoI GCNNNNNNNGC 1 cut(s) 238
NciI CCSGG 1 cut(s) 180
NdeII GATC 1 cut(s) 421
NlaIII CATG 4 cut(s) 288, 374, 408, 604
NmeAIII GCCGAG 1 cut(s) 187
NmuCI GTSAC 4 cut(s) 169, 445, 755, 845
PaqCI CACCTGC 1 cut(s) 713
PctI GAATGC 1 cut(s) 368
PdmI GAANNNNTTC 1 cut(s) 253
PfeI GAWTC 2 cut(s) 89, 121
PkrI GCNGC 2 cut(s) 240, 510
PleI GAGTC 1 cut(s) 297
PmaCI CACGTG 1 cut(s) 468
PmlI CACGTG 1 cut(s) 468
PpsI GAGTC 1 cut(s) 297
Ppu21I YACGTR 2 cut(s) 68, 468
PsiI TTATAA 1 cut(s) 977
Psp6I CCWGG 2 cut(s) 385, 801
PspCI CACGTG 1 cut(s) 468
PspGI CCWGG 2 cut(s) 385, 801
PspPI GGNCC 2 cut(s) 34, 198
RsaI GTAC 3 cut(s) 66, 568, 749
RsaNI GTAC 3 cut(s) 65, 567, 748
RseI CAYNNNNRTG 4 cut(s) 375, 409, 900, 912
SaqAI TTAA 3 cut(s) 563, 684, 821
SatI GCNGC 2 cut(s) 239, 509
Sau3AI GATC 1 cut(s) 421
Sau96I GGNCC 2 cut(s) 34, 198
ScaI AGTACT 1 cut(s) 568
SchI GAGTC 1 cut(s) 298
ScrFI CCNGG 3 cut(s) 180, 387, 803
SduI GDGCHC 1 cut(s) 76
SfaNI GCATC 1 cut(s) 892
SmiMI CAYNNNNRTG 4 cut(s) 375, 409, 900, 912
SpeI ACTAGT 1 cut(s) 542
SspI AATATT 1 cut(s) 561
SspMI CTAG 2 cut(s) 543, 843
StyD4I CCNGG 3 cut(s) 178, 385, 801
StyI CCWWGG 2 cut(s) 391, 751
TaaI ACNGT 5 cut(s) 193, 265, 302, 402, 831
TaiI ACGT 2 cut(s) 70, 470
TaqI TCGA 1 cut(s) 666
TaqII GACCGA 1 cut(s) 867
TatI WGTACW 1 cut(s) 566
TfiI GAWTC 2 cut(s) 89, 121
Tru1I TTAA 3 cut(s) 563, 684, 821
Tru9I TTAA 3 cut(s) 563, 684, 821
TscAI CASTG 3 cut(s) 196, 270, 454
TseFI GTSAC 4 cut(s) 169, 445, 755, 845
TseI GCWGC 2 cut(s) 238, 508
Tsp45I GTSAC 4 cut(s) 169, 445, 755, 845
TspDTI ATGAA 5 cut(s) 174, 273, 393, 695, 779
TspRI CASTG 3 cut(s) 196, 270, 454
XapI RAATTY 4 cut(s) 138, 249, 702, 786
XcmI CCANNNNNNNNNTGG 2 cut(s) 426, 931
XmiI GTMKAC 1 cut(s) 397
XmnI GAANNNNTTC 1 cut(s) 253
XspI CTAG 2 cut(s) 543, 843
ZrmI AGTACT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.