pycom09g06670

WD repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0004773
Physical Location & Seq
Reverse (-)
4910338 .. 4914273
3936 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g06670.1

Sequence Viewer

Length: 1740 bp
ATGGGAGGTGTGGGGGATGAAGAACCAGCCACAAAACGCATGAAATTATCCGCTGGAGAATTGAGAGGACTTTCCAACGGTTCATCTTGTGCAGAGCCTGTAGCTGGCTCTTCAAGAGACTTGATGGCTCGGCCCCTACCATCTGAAGGGGACGAACAGGTTGTTGGTTCAAAAGGAGTTATTAAGAGGGTGGAATTTGTTCGACTAATAGCTAAGGCGCTATATTCTCTTGGCTATAAGAAAAGTGGTGCTCATTTGGAAGAAGAGTCTGGGATACCATTGCATTCTTCTTTCGTAAATCTCTTTATGCAGCAAATTCTGGATGGAAACTGGGATGGAAGTGTTGACACATTGCAAAAAATTGGTCTATCTGATGAAACCATTGTTAAATCAGCCTCCTTTATGATATTGGAGCAGAAGTACTTTGAACTTCTAGATGAAGAAAAAGACATGGATGCATTGAAGACATTGAGGACTGAGATTGTGCCTCTCTGCATCAATGGTAGTAGAGTTCGCGAGCTTTCTTCCTGCATAGTTTCTCCTTCATTTGGTGGGACTCCTGGTAAGAACATTGTGCGGGCCAAGTCTCGATCAAAGTTACTGGAGGACTTGCAGAAACTGCTTCCCCCAACACTTATGATACTTGACAGACGGTTGGAACATTTGGTTGAACAGGCCCTTGTCTTACAACGTGATGCTTGCATTTTTCACAACACCTTGAACGAGGAAATGTCATTGTACACCGATCATTGGTGTGGGAAAGAGCAGATTCCTTCTCAAACGTTACAGATATTACAGGCACACAATGACGAAGTCTGGTTTCTGCAATTTTCACACGATGGGAAGTACTTAGCTTCATCATCAAGTGATCAATCAGCAATCATATGGGAGGTTGATGTGAATGGTGGGGTTTCTTTAAAGCATAGACTATCTGGTCACCAGAAACCTGTTTCCTCAGTTTCATGGAGTCCTGATGACCATCAACTCCTTACTTGTGGAGTGGAGGAGGCTGTCCGGCGTTGGGATGTTTCTTCTGGTGAATGTCTCCGTGTTTATGAAAAACCTGGTGTTGGTCTGGTGTCCTGTGGATGGTTTCCAGATGGGAAATGTTTATTCTCTGGTGTCAGTGACAAGAGTATCTGCATGTGGGAGTTAGATGGGAAAGAGCTGGAGTGTTGGAAAGGCCAACGAAGTTTAAGAATCTCTGATTTGGAAATAACTAGCGATGGGAAGCAACTTATAAGTAATTGTAGAGAGAATGCTATATTACTCCTTGACAGGGAAGCCAAAGTTGAGACATTTATTGAAGAAGATCAAACTATAACATCTTTTTCTTTATCAAGAGATAATAGGTTCTTGTTGGTTAATCTTCTGAACCAAGAAATCCATCTCTGGAACATAGAAGGTGAAAACAAGCTTGTCGCTAAGTACAAAGGTCACAAACGCACCCGATTTGTTATCAGGTCTTGTTTTGGTGGACTTGAAGAAGCTTTTGTTGCGAGCGGTAGTGAGGATTCACAGGTTTACATATGGCATAGAGGCTCAGGGGAGCTCATAGAGGCATTGCCGGGTCATTCTGGAGCTGTTAATTGTGTCAGCTGGAATCCAGCAAACCCGCACATGTTCGCCTCTGCCAGTGATGACCGCACAATTCGGATATGGGGCCTGAGCGGGCAAAAGGTGAAGCACAGGGGTGCACACAGCAATGGCATCCATTTAAATTGTAATGGAGGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WD40

Protein Analysis

580

Amino Acids

64.3

Weight (kDa)

5.76

Isoelectric Point (pI)

50.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LisH_WDR26 PF23627 53 - 96 3e-22 WDR26 Lis-homology motif
Beta-prop_RIG_2nd PF23775 220 - 366 5.3e-07 RIG second beta-propeller
WD40_Prp19 PF24814 259 - 483 1.4e-17 Prp19 WD40 domain
WD40 PF00400 260 - 296 2.5e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 260 - 350 1.6e-10 CAF1B/HIR1 beta-propeller domain
Beta-prop_WDR3_1st PF25173 260 - 385 2.7e-21 WDR3 first beta-propeller domain
Beta-prop_EML PF23409 260 - 429 3.8e-08 Echinoderm microtubule-associated protein first beta-propeller
WD40_CDC20-Fz PF24807 260 - 350 2.5e-13 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 261 - 350 6.9e-11 TEP-1 second beta-propeller
Beta-prop_IFT122_1st PF23381 261 - 389 1.7e-10 IFT122 first beta-propeller
Beta-prop_EML_2 PF23414 263 - 327 8.8e-06 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR5 PF25175 265 - 554 2.2e-55 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 268 - 518 2.3e-34 THOC3 beta-propeller domain
WD40_Gbeta PF25391 278 - 511 8.6e-11 G protein beta WD-40 repeat protein
WD40 PF00400 306 - 342 6e-06 WD domain, G-beta repeat
WDR55 PF24796 307 - 556 4e-13 WDR55
Beta-prop_WDR3_2nd PF25172 308 - 426 2.7e-06 WDR3 second beta-propeller domain
Beta-prop_TEP1_2nd PF25047 310 - 440 1.1e-06 TEP-1 second beta-propeller
Beta-prop_Aladin PF25460 314 - 424 5.1e-10 Aladin seven-bladed propeller
Beta-prop_EML_2 PF23414 324 - 474 1.3e-12 Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_2nd PF25172 345 - 555 4e-10 WDR3 second beta-propeller domain
WD40_CDC20-Fz PF24807 360 - 481 1.9e-06 CDC20/Fizzy WD40 domain
Beta-prop_TEP1_2nd PF25047 369 - 571 1.2e-07 TEP-1 second beta-propeller
Beta-prop_WDR19_1st PF23389 409 - 566 4.1e-06 WDR19 first beta-propeller
Beta-prop_WDR3_1st PF25173 427 - 561 2.7e-16 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 495 - 566 3.3e-07 CAF1B/HIR1 beta-propeller domain
EIF3I PF24805 496 - 562 1.3e-06 EIF3I
WD40_WDHD1_1st PF24817 496 - 563 5.3e-06 WDHD1 first WD40 domain
WD40_Prp19 PF24814 496 - 564 2.8e-11 Prp19 WD40 domain
WD40_CDC20-Fz PF24807 496 - 560 1.8e-09 CDC20/Fizzy WD40 domain
Beta-prop_THOC3 PF25174 498 - 562 3.9e-14 THOC3 beta-propeller domain
Beta-prop_WDR5 PF25175 515 - 569 2.2e-07 WDR5 beta-propeller domain
WD40 PF00400 517 - 554 1.6e-09 WD domain, G-beta repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1241
AasI GACNNNNNNGTC 1 cut(s) 933
AccBSI CCGCTC 2 cut(s) 1503, 1671
AccII CGCG 1 cut(s) 516
AciI CCGC 6 cut(s) 51, 577, 1503, 1616, 1645, 1671
AclI AACGTT 1 cut(s) 782
AcsI RAATTY 2 cut(s) 194, 315
AcuI CTGAAG 1 cut(s) 165
AfaI GTAC 4 cut(s) 422, 740, 848, 1430
AfiI CCNNNNNNNGG 8 cut(s) 104, 146, 548, 750, 1021, 1070, 1089, 1279
AflIII ACRYGT 1 cut(s) 1620
AgsI TTSAA 8 cut(s) 114, 171, 428, 463, 671, 721, 1307, 1484
AjnI CCWGG 2 cut(s) 559, 1063
AjuI GAANNNNNNNTTGG 2 cut(s) 147, 179
AleI CACNNNNGTG 1 cut(s) 1692
Alw21I GWGCWC 3 cut(s) 253, 1554, 1699
Alw26I GTCTC 4 cut(s) 111, 591, 1049, 1289
Alw44I GTGCAC 1 cut(s) 1695
AlwNI CAGNNNCTG 2 cut(s) 98, 619
AoxI GGCC 5 cut(s) 131, 579, 675, 1183, 1663
ApaLI GTGCAC 1 cut(s) 1695
ApeKI GCWGC 1 cut(s) 310
ApoI RAATTY 2 cut(s) 194, 315
ArsI GACNNNNNNTTYG 2 cut(s) 349, 381
Asp700I GAANNNNTTC 1 cut(s) 198
AspLEI GCGC 1 cut(s) 220
AspS9I GGNCC 4 cut(s) 132, 579, 676, 1663
AsuC2I CCSGG 1 cut(s) 1569
AsuHPI GGTGA 4 cut(s) 929, 1049, 1418, 1693
BaeGI GKGCMC 1 cut(s) 1699
BaeI ACNNNNGTAYC 2 cut(s) 1120, 1153
BanII GRGCYC 1 cut(s) 1554
BbsI GAAGAC 1 cut(s) 470
Bbv12I GWGCWC 3 cut(s) 253, 1554, 1699
BbvI GCAGC 1 cut(s) 322
BciT130I CCWGG 2 cut(s) 561, 1065
BciVI GTATCC 1 cut(s) 267
BclI TGATCA 1 cut(s) 868
BcnI CCSGG 1 cut(s) 1569
BcoDI GTCTC 4 cut(s) 111, 591, 1049, 1289
BfaI CTAG 2 cut(s) 434, 1221
BfmI CTRYAG 1 cut(s) 99
BfoI RGCGCY 1 cut(s) 221
BfuI GTATCC 1 cut(s) 267
BisI GCNGC 1 cut(s) 311
BlsI GCNGC 1 cut(s) 312
BmcAI AGTACT 2 cut(s) 422, 848
Bme1390I CCNGG 3 cut(s) 561, 1065, 1569
BmgT120I GGNCC 4 cut(s) 132, 579, 676, 1663
BmiI GGNNCC 2 cut(s) 134, 1664
BmrFI CCNGG 3 cut(s) 561, 1065, 1569
BmrI ACTGGG 1 cut(s) 340
BmsI GCATC 4 cut(s) 445, 504, 685, 1719
BmuI ACTGGG 1 cut(s) 340
BpiI GAAGAC 1 cut(s) 470
BpmI CTGGAG 4 cut(s) 75, 623, 1190, 1599
Bpu10I CCTNAGC 3 cut(s) 213, 1543, 1667
BpuMI CCSGG 1 cut(s) 1569
BsaBI GATNNNNATC 1 cut(s) 978
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 8 cut(s) 104, 146, 548, 750, 1021, 1070, 1089, 1279
Bse1I ACTGG 3 cut(s) 335, 606, 1635
Bse3DI GCAATG 4 cut(s) 278, 350, 1562, 1711
Bse8I GATNNNNATC 1 cut(s) 978
BseBI CCWGG 2 cut(s) 561, 1065
BseGI GGATG 7 cut(s) 22, 328, 340, 460, 1030, 1094, 1710
BseJI GATNNNNATC 1 cut(s) 978
BseLI CCNNNNNNNGG 8 cut(s) 104, 146, 548, 750, 1021, 1070, 1089, 1279
BseMI GCAATG 4 cut(s) 278, 350, 1562, 1711
BseMII CTCAG 4 cut(s) 468, 969, 1557, 1658
BseNI ACTGG 3 cut(s) 335, 606, 1635
BseRI GAGGAG 1 cut(s) 1019
BseSI GKGCMC 1 cut(s) 1699
BseXI GCAGC 1 cut(s) 322
BsgI GTGCAG 1 cut(s) 111
Bsh1236I CGCG 1 cut(s) 516
BshFI GGCC 5 cut(s) 133, 581, 677, 1185, 1665
BsiHKAI GWGCWC 3 cut(s) 253, 1554, 1699
BsiSI CCGG 2 cut(s) 1015, 1568
BslFI GGGAC 2 cut(s) 164, 568
BslI CCNNNNNNNGG 8 cut(s) 104, 146, 548, 750, 1021, 1070, 1089, 1279
BsmAI GTCTC 4 cut(s) 111, 591, 1049, 1289
BsmFI GGGAC 2 cut(s) 164, 568
BsmI GAATGC 2 cut(s) 283, 1264
BsnI GGCC 5 cut(s) 133, 581, 677, 1185, 1665
Bsp1286I GDGCHC 3 cut(s) 253, 1554, 1699
Bsp1407I TGTACA 1 cut(s) 738
Bsp143I GATC 4 cut(s) 590, 745, 868, 1312
Bsp68I TCGCGA 1 cut(s) 516
BspACI CCGC 6 cut(s) 51, 577, 1503, 1616, 1645, 1671
BspANI GGCC 5 cut(s) 133, 581, 677, 1185, 1665
BspCNI CTCAG 4 cut(s) 469, 968, 1556, 1659
BspFNI CGCG 1 cut(s) 516
BspLI GGNNCC 2 cut(s) 134, 1664
BspQI GCTCTTC 1 cut(s) 115
BsrBI CCGCTC 2 cut(s) 1503, 1671
BsrDI GCAATG 4 cut(s) 278, 350, 1562, 1711
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 3 cut(s) 335, 606, 1635
BssMI GATC 4 cut(s) 590, 745, 868, 1312
Bst2UI CCWGG 2 cut(s) 561, 1065
Bst4CI ACNGT 2 cut(s) 80, 654
Bst6I CTCTTC 2 cut(s) 115, 258
BstAPI GCANNNNNTGC 1 cut(s) 619
BstAUI TGTACA 1 cut(s) 738
BstC8I GCNNGC 6 cut(s) 106, 518, 579, 700, 1501, 1673
BstDEI CTNAG 7 cut(s) 213, 477, 850, 955, 1425, 1543, 1667
BstEII GGTNACC 1 cut(s) 935
BstF5I GGATG 7 cut(s) 22, 328, 340, 460, 1030, 1094, 1710
BstFNI CGCG 1 cut(s) 516
BstH2I RGCGCY 1 cut(s) 221
BstHHI GCGC 1 cut(s) 220
BstKTI GATC 4 cut(s) 593, 748, 871, 1315
BstMAI GTCTC 4 cut(s) 111, 591, 1049, 1289
BstMBI GATC 4 cut(s) 590, 745, 868, 1312
BstMWI GCNNNNNNNGC 2 cut(s) 619, 1496
BstNI CCWGG 2 cut(s) 561, 1065
BstNSI RCATGY 2 cut(s) 1147, 1624
BstPI GGTNACC 1 cut(s) 935
BstSCI CCNGG 3 cut(s) 559, 1063, 1567
BstSFI CTRYAG 1 cut(s) 99
BstSLI GKGCMC 1 cut(s) 1699
BstUI CGCG 1 cut(s) 516
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 1 cut(s) 470
BsuI GTATCC 1 cut(s) 267
BsuRI GGCC 5 cut(s) 133, 581, 677, 1185, 1665
BtgZI GCGATG 1 cut(s) 1239
BtsCI GGATG 7 cut(s) 22, 328, 340, 460, 1030, 1094, 1710
BtsIMutI CAGTG 2 cut(s) 1132, 1642
BtuMI TCGCGA 1 cut(s) 516
Cac8I GCNNGC 6 cut(s) 106, 518, 579, 700, 1501, 1673
CaiI CAGNNNCTG 2 cut(s) 98, 619
CfoI GCGC 1 cut(s) 220
Cfr13I GGNCC 4 cut(s) 132, 579, 676, 1663
CsiI ACCWGGT 1 cut(s) 1063
Csp6I GTAC 4 cut(s) 421, 739, 847, 1429
CviAII CATG 6 cut(s) 40, 451, 963, 1144, 1621, 1737
CviQI GTAC 4 cut(s) 421, 739, 847, 1429
DdeI CTNAG 7 cut(s) 213, 477, 850, 955, 1425, 1543, 1667
DpnI GATC 4 cut(s) 592, 747, 870, 1314
DpnII GATC 4 cut(s) 590, 745, 868, 1312
DraI TTTAAA 2 cut(s) 918, 1719
DrdI GACNNNNNNGTC 1 cut(s) 933
DseDI GACNNNNNNGTC 1 cut(s) 933
Eam1104I CTCTTC 2 cut(s) 115, 258
EarI CTCTTC 2 cut(s) 115, 258
Ecl136II GAGCTC 1 cut(s) 1552
Eco24I GRGCYC 1 cut(s) 1554
Eco53kI GAGCTC 1 cut(s) 1552
Eco57I CTGAAG 1 cut(s) 165
Eco91I GGTNACC 1 cut(s) 935
EcoICRI GAGCTC 1 cut(s) 1552
EcoO109I RGGNCCY 2 cut(s) 676, 1663
EcoO65I GGTNACC 1 cut(s) 935
EcoRII CCWGG 2 cut(s) 559, 1063
EcoT22I ATGCAT 1 cut(s) 460
EcoT38I GRGCYC 1 cut(s) 1554
FaeI CATG 6 cut(s) 43, 454, 966, 1147, 1624, 1740
FaqI GGGAC 2 cut(s) 164, 568
FatI CATG 6 cut(s) 39, 450, 962, 1143, 1620, 1736
FauI CCCGC 3 cut(s) 570, 1623, 1664
FauNDI CATATG 2 cut(s) 884, 1529
FbaI TGATCA 1 cut(s) 868
Fnu4HI GCNGC 1 cut(s) 311
FokI GGATG 7 cut(s) 29, 335, 347, 467, 1037, 1101, 1697
FriOI GRGCYC 1 cut(s) 1554
Fsp4HI GCNGC 1 cut(s) 311
FspBI CTAG 2 cut(s) 434, 1221
GlaI GCGC 1 cut(s) 219
GluI GCNGC 1 cut(s) 311
GsuI CTGGAG 4 cut(s) 75, 623, 1190, 1599
HaeII RGCGCY 1 cut(s) 221
HaeIII GGCC 5 cut(s) 133, 581, 677, 1185, 1665
HapII CCGG 2 cut(s) 1015, 1568
HhaI GCGC 1 cut(s) 220
Hin1II CATG 6 cut(s) 43, 454, 966, 1147, 1624, 1740
Hin6I GCGC 1 cut(s) 218
HinP1I GCGC 1 cut(s) 218
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 2 cut(s) 1415, 1488
HinfI GANTC 7 cut(s) 266, 556, 769, 967, 1200, 1514, 1603
HpaII CCGG 2 cut(s) 1015, 1568
HphI GGTGA 4 cut(s) 929, 1049, 1418, 1693
Hpy166II GTNNAC 5 cut(s) 346, 741, 1478, 1525, 1697
Hpy188I TCNGA 5 cut(s) 145, 373, 1207, 1374, 1656
Hpy8I GTNNAC 5 cut(s) 346, 741, 1478, 1525, 1697
HpyAV CCTTC 4 cut(s) 140, 552, 783, 1397
HpyCH4III ACNGT 2 cut(s) 80, 654
HpyCH4IV ACGT 2 cut(s) 691, 782
HpyF10VI GCNNNNNNNGC 2 cut(s) 619, 1496
HpyF3I CTNAG 7 cut(s) 213, 477, 850, 955, 1425, 1543, 1667
HpySE526I ACGT 2 cut(s) 691, 782
Hsp92II CATG 6 cut(s) 43, 454, 966, 1147, 1624, 1740
HspAI GCGC 1 cut(s) 218
Ksp22I TGATCA 1 cut(s) 868
Kzo9I GATC 4 cut(s) 590, 745, 868, 1312
LguI GCTCTTC 1 cut(s) 115
LmnI GCTCC 3 cut(s) 412, 1549, 1580
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 4 cut(s) 445, 504, 685, 1719
MabI ACCWGGT 1 cut(s) 1063
MaeI CTAG 2 cut(s) 434, 1221
MaeII ACGT 2 cut(s) 691, 782
MaeIII GTNAC 5 cut(s) 597, 783, 935, 1127, 1436
MalI GATC 4 cut(s) 592, 747, 870, 1314
MbiI CCGCTC 2 cut(s) 1503, 1671
MboI GATC 4 cut(s) 590, 745, 868, 1312
MhlI GDGCHC 3 cut(s) 253, 1554, 1699
MlyI GAGTC 3 cut(s) 275, 550, 976
MmeI TCCRAC 3 cut(s) 99, 636, 1157
Mph1103I ATGCAT 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 198
MseI TTAA 7 cut(s) 183, 387, 917, 1196, 1365, 1587, 1718
MslI CAYNNNNRTG 3 cut(s) 753, 1692, 1704
MspA1I CMGCKG 2 cut(s) 53, 1599
MspI CCGG 2 cut(s) 1015, 1568
MspR9I CCNGG 3 cut(s) 561, 1065, 1569
Mva1269I GAATGC 2 cut(s) 283, 1264
MvaI CCWGG 2 cut(s) 561, 1065
MvnI CGCG 1 cut(s) 516
MwoI GCNNNNNNNGC 2 cut(s) 619, 1496
NciI CCSGG 1 cut(s) 1569
NdeI CATATG 2 cut(s) 884, 1529
NdeII GATC 4 cut(s) 590, 745, 868, 1312
NlaIII CATG 6 cut(s) 43, 454, 966, 1147, 1624, 1740
NlaIV GGNNCC 2 cut(s) 134, 1664
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 3 cut(s) 935, 1127, 1436
NruI TCGCGA 1 cut(s) 516
NsiI ATGCAT 1 cut(s) 460
NspI RCATGY 2 cut(s) 1147, 1624
OliI CACNNNNGTG 1 cut(s) 1692
PciI ACATGT 1 cut(s) 1620
PciSI GCTCTTC 1 cut(s) 115
PctI GAATGC 2 cut(s) 283, 1264
PdmI GAANNNNTTC 1 cut(s) 198
PfeI GAWTC 4 cut(s) 769, 1200, 1514, 1603
PflFI GACNNNGTC 1 cut(s) 812
PkrI GCNGC 1 cut(s) 312
PleI GAGTC 3 cut(s) 274, 550, 975
PpsI GAGTC 3 cut(s) 274, 550, 975
PscI ACATGT 1 cut(s) 1620
PsiI TTATAA 1 cut(s) 1241
Psp124BI GAGCTC 1 cut(s) 1554
Psp1406I AACGTT 1 cut(s) 782
Psp6I CCWGG 2 cut(s) 559, 1063
PspEI GGTNACC 1 cut(s) 935
PspGI CCWGG 2 cut(s) 559, 1063
PspN4I GGNNCC 2 cut(s) 134, 1664
PspPI GGNCC 4 cut(s) 132, 579, 676, 1663
PstNI CAGNNNCTG 2 cut(s) 98, 619
PsyI GACNNNGTC 1 cut(s) 812
PvuII CAGCTG 1 cut(s) 1599
RruI TCGCGA 1 cut(s) 516
RsaI GTAC 4 cut(s) 422, 740, 848, 1430
RsaNI GTAC 4 cut(s) 421, 739, 847, 1429
RseI CAYNNNNRTG 3 cut(s) 753, 1692, 1704
SacI GAGCTC 1 cut(s) 1554
SapI GCTCTTC 1 cut(s) 115
SaqAI TTAA 7 cut(s) 183, 387, 917, 1196, 1365, 1587, 1718
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 4 cut(s) 590, 745, 868, 1312
Sau96I GGNCC 4 cut(s) 132, 579, 676, 1663
ScaI AGTACT 2 cut(s) 422, 848
SchI GAGTC 3 cut(s) 275, 550, 976
ScrFI CCNGG 3 cut(s) 561, 1065, 1569
SduI GDGCHC 3 cut(s) 253, 1554, 1699
SexAI ACCWGGT 1 cut(s) 1063
SfaNI GCATC 4 cut(s) 445, 504, 685, 1719
SfcI CTRYAG 1 cut(s) 99
SmiI ATTTAAAT 1 cut(s) 1719
SmiMI CAYNNNNRTG 3 cut(s) 753, 1692, 1704
SsiI CCGC 6 cut(s) 51, 577, 1503, 1616, 1645, 1671
SspMI CTAG 2 cut(s) 434, 1221
SstI GAGCTC 1 cut(s) 1554
StyD4I CCNGG 3 cut(s) 559, 1063, 1567
SwaI ATTTAAAT 1 cut(s) 1719
TaaI ACNGT 2 cut(s) 80, 654
TaiI ACGT 2 cut(s) 694, 785
TaqI TCGA 2 cut(s) 202, 589
TatI WGTACW 4 cut(s) 420, 738, 846, 1428
TfiI GAWTC 4 cut(s) 769, 1200, 1514, 1603
Tru1I TTAA 7 cut(s) 183, 387, 917, 1196, 1365, 1587, 1718
Tru9I TTAA 7 cut(s) 183, 387, 917, 1196, 1365, 1587, 1718
TscAI CASTG 2 cut(s) 1132, 1642
TseFI GTSAC 3 cut(s) 935, 1127, 1436
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 3 cut(s) 935, 1127, 1436
TspDTI ATGAA 9 cut(s) 33, 56, 72, 390, 453, 534, 846, 951, 1071
TspGWI ACGGA 1 cut(s) 1037
TspRI CASTG 2 cut(s) 1132, 1642
Tth111I GACNNNGTC 1 cut(s) 812
VneI GTGCAC 1 cut(s) 1695
XapI RAATTY 2 cut(s) 194, 315
XbaI TCTAGA 1 cut(s) 433
XceI RCATGY 2 cut(s) 1147, 1624
XmnI GAANNNNTTC 1 cut(s) 198
XspI CTAG 2 cut(s) 434, 1221
ZrmI AGTACT 2 cut(s) 422, 848
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.