AT5G10710

Cenp-O kinetochore centromere component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008371
Physical Location & Seq
Forward (+)
3378956 .. 3382958
4003 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G10710.3

Sequence Viewer

Length: 939 bp
ATGGGAGAAATGATTGTTTCAATGGACCAAGATATCCGACTGGATACAACACGAGCAAGATTGTCAAACCTTCTCAAGAGGCATCGAGAATTGTCAGACCGTCTTACTAGGGATTCTGATAAGACAATGTTAGATCGCTTAAACAAAGAATTTGAAGCTGCACGGAGATCCCAAAGTCAGGAAGTCTTCTTAGATGGGGAAGAGTGGAATGATGGTTTGTTGGCCACATTAAGGGAACGGGTGCATATGGAAGCTGACAGAAAGGCAGATAACGGTAATGCAGGTTTTTCACTAGTTTGTCATCCTGAAGAACGAATTACTTACAGAGTGGGAAATAAGGTGATATGTTGCCTAGATGGATCGAGAATTGGAATACAGTTTGAAACATCTACTGCAGGAGAAACTTACGAGGTTTACCACTGTGTGCTTGAGAGCAAGTCGTTTTTGGAGAAGATGATTGTGCTTGAGCACACAATTCCTTTCTTTTTGCCGCTAAGTGACTTGGAAAATGATCTTCTTTTTTCGAATGCTAAGAAATTCATCGATAATGTTGGGGATCTCCTGCAAGCATATGTGGACAGAAAGGAACAGGTCCGGCTTATCAAAGAGCTCTTTGGACATCAGATCAGTGAGATTTATCACAGTCTTCCTTACCACATGATTGAATTTTCTATGGATGATTGTGACTGCAAGTTCGTGGTGAGCCTTAGATATGGAGATCTTCTGTGTGAACTTCCGACAAAAGTGAGAATTCTAGTATGGCCAATGCATCATCTGTCGAAGAAACAGTGTACAAGCCCTGGAAGTCCAGCAATCCCTGTGCGTTTACCATTTGCCGAGGATGCTTTCCGGATCCAATCACTACCTGAAGCATACGCAGAGATTATGCCGAACATGCCGAATGAAATTCGGCAATTATTTCAGACTAGTCCAAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CENP-O

Protein Analysis

312

Amino Acids

36.02

Weight (kDa)

5.45

Isoelectric Point (pI)

46.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CENP-O PF09496 104 - 292 6.1e-30 Cenp-O kinetochore centromere component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 272
AccIII TCCGGA 1 cut(s) 849
AciI CCGC 1 cut(s) 491
AclWI GGATC 5 cut(s) 162, 367, 564, 847, 860
AcoI YGGCCR 2 cut(s) 222, 761
AcsI RAATTY 5 cut(s) 149, 536, 665, 750, 906
AcuI CTGAAG 2 cut(s) 327, 888
AdeI CACNNNGTG 1 cut(s) 424
AfaI GTAC 1 cut(s) 793
AfiI CCNNNNNNNGG 2 cut(s) 178, 231
AgsI TTSAA 4 cut(s) 21, 155, 383, 665
AhlI ACTAGT 2 cut(s) 292, 926
AjnI CCWGG 1 cut(s) 799
AluBI AGCT 4 cut(s) 158, 254, 610, 936
AluI AGCT 4 cut(s) 158, 254, 610, 936
Alw21I GWGCWC 2 cut(s) 471, 612
AlwI GGATC 5 cut(s) 162, 367, 564, 847, 860
Aor13HI TCCGGA 1 cut(s) 849
AoxI GGCC 2 cut(s) 222, 761
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 5 cut(s) 149, 536, 665, 750, 906
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
AspS9I GGNCC 2 cut(s) 25, 592
AsuHPI GGTGA 2 cut(s) 352, 712
AsuII TTCGAA 1 cut(s) 524
AvaII GGWCC 2 cut(s) 25, 592
BalI TGGCCA 2 cut(s) 224, 763
BamHI GGATCC 1 cut(s) 852
BanII GRGCYC 1 cut(s) 612
BauI CACGAG 1 cut(s) 51
BbsI GAAGAC 2 cut(s) 178, 638
Bbv12I GWGCWC 2 cut(s) 471, 612
BbvI GCAGC 1 cut(s) 145
BccI CCATC 3 cut(s) 188, 206, 350
BciT130I CCWGG 1 cut(s) 801
BciVI GTATCC 1 cut(s) 37
BcuI ACTAGT 2 cut(s) 292, 926
BfaI CTAG 5 cut(s) 108, 293, 353, 755, 927
BfmI CTRYAG 1 cut(s) 393
BfuAI ACCTGC 1 cut(s) 272
BfuI GTATCC 1 cut(s) 37
BglII AGATCT 1 cut(s) 718
BisI GCNGC 2 cut(s) 159, 491
BlsI GCNGC 2 cut(s) 160, 492
Bme1390I CCNGG 1 cut(s) 801
Bme18I GGWCC 2 cut(s) 25, 592
BmgT120I GGNCC 2 cut(s) 25, 592
BmiI GGNNCC 1 cut(s) 854
BmrFI CCNGG 1 cut(s) 801
BmsI GCATC 3 cut(s) 91, 778, 832
BpiI GAAGAC 2 cut(s) 178, 638
Bpu14I TTCGAA 1 cut(s) 524
BpuEI CTTGAG 3 cut(s) 59, 449, 485
Bsa29I ATCGAT 1 cut(s) 543
BsaJI CCNNGG 2 cut(s) 799, 837
BsaWI WCCGGW 1 cut(s) 849
Bsc4I CCNNNNNNNGG 2 cut(s) 178, 231
Bse1I ACTGG 1 cut(s) 45
BseAI TCCGGA 1 cut(s) 849
BseBI CCWGG 1 cut(s) 801
BseCI ATCGAT 1 cut(s) 543
BseDI CCNNGG 2 cut(s) 799, 837
BseGI GGATG 3 cut(s) 301, 682, 847
BseLI CCNNNNNNNGG 2 cut(s) 178, 231
BseNI ACTGG 1 cut(s) 45
BseXI GCAGC 1 cut(s) 145
BsgI GTGCAG 1 cut(s) 144
BshFI GGCC 2 cut(s) 224, 763
BshVI ATCGAT 1 cut(s) 543
BsiHKAI GWGCWC 2 cut(s) 471, 612
BsiSI CCGG 2 cut(s) 595, 850
BslI CCNNNNNNNGG 2 cut(s) 178, 231
BsmI GAATGC 1 cut(s) 532
BsnI GGCC 2 cut(s) 224, 763
Bsp119I TTCGAA 1 cut(s) 524
Bsp1286I GDGCHC 2 cut(s) 471, 612
Bsp13I TCCGGA 1 cut(s) 849
Bsp1407I TGTACA 1 cut(s) 791
Bsp143I GATC 8 cut(s) 133, 167, 359, 511, 556, 624, 718, 852
BspACI CCGC 1 cut(s) 491
BspANI GGCC 2 cut(s) 224, 763
BspDI ATCGAT 1 cut(s) 543
BspEI TCCGGA 1 cut(s) 849
BspLI GGNNCC 1 cut(s) 854
BspMAI CTGCAG 1 cut(s) 397
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 5 cut(s) 162, 367, 564, 847, 860
BspT104I TTCGAA 1 cut(s) 524
BsrGI TGTACA 1 cut(s) 791
BsrI ACTGG 1 cut(s) 45
BssECI CCNNGG 2 cut(s) 799, 837
BssMI GATC 8 cut(s) 133, 167, 359, 511, 556, 624, 718, 852
BssSI CACGAG 1 cut(s) 51
Bst2BI CACGAG 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 801
Bst4CI ACNGT 6 cut(s) 101, 275, 378, 422, 644, 789
Bst6I CTCTTC 1 cut(s) 195
BstAUI TGTACA 1 cut(s) 791
BstBI TTCGAA 1 cut(s) 524
BstC8I GCNNGC 1 cut(s) 567
BstDEI CTNAG 4 cut(s) 190, 494, 531, 707
BstF5I GGATG 3 cut(s) 301, 682, 847
BstKTI GATC 8 cut(s) 136, 170, 362, 514, 559, 627, 721, 855
BstMBI GATC 8 cut(s) 133, 167, 359, 511, 556, 624, 718, 852
BstMWI GCNNNNNNNGC 2 cut(s) 842, 895
BstNI CCWGG 1 cut(s) 801
BstNSI RCATGY 1 cut(s) 898
BstSCI CCNGG 1 cut(s) 799
BstSFI CTRYAG 1 cut(s) 393
BstV1I GCAGC 1 cut(s) 145
BstV2I GAAGAC 2 cut(s) 178, 638
BstX2I RGATCY 4 cut(s) 167, 556, 718, 852
BstYI RGATCY 4 cut(s) 167, 556, 718, 852
Bsu15I ATCGAT 1 cut(s) 543
BsuI GTATCC 1 cut(s) 37
BsuRI GGCC 2 cut(s) 224, 763
BsuTUI ATCGAT 1 cut(s) 543
BtsCI GGATG 3 cut(s) 301, 682, 847
BtsIMutI CAGTG 3 cut(s) 418, 634, 794
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 1 cut(s) 567
Cfr13I GGNCC 2 cut(s) 25, 592
ClaI ATCGAT 1 cut(s) 543
Csp6I GTAC 1 cut(s) 792
CviAII CATG 2 cut(s) 658, 895
CviJI RGCY 9 cut(s) 158, 224, 254, 598, 610, 705, 763, 798, 936
CviKI_1 RGCY 9 cut(s) 158, 224, 254, 598, 610, 705, 763, 798, 936
CviQI GTAC 1 cut(s) 792
DdeI CTNAG 4 cut(s) 190, 494, 531, 707
DpnI GATC 8 cut(s) 135, 169, 361, 513, 558, 626, 720, 854
DpnII GATC 8 cut(s) 133, 167, 359, 511, 556, 624, 718, 852
DraIII CACNNNGTG 1 cut(s) 424
EaeI YGGCCR 2 cut(s) 222, 761
Eam1104I CTCTTC 1 cut(s) 195
EarI CTCTTC 1 cut(s) 195
Ecl136II GAGCTC 1 cut(s) 610
Eco24I GRGCYC 1 cut(s) 612
Eco32I GATATC 1 cut(s) 34
Eco47I GGWCC 2 cut(s) 25, 592
Eco53kI GAGCTC 1 cut(s) 610
Eco57I CTGAAG 2 cut(s) 327, 888
EcoICRI GAGCTC 1 cut(s) 610
EcoRI GAATTC 1 cut(s) 750
EcoRII CCWGG 1 cut(s) 799
EcoRV GATATC 1 cut(s) 34
EcoT22I ATGCAT 1 cut(s) 771
EcoT38I GRGCYC 1 cut(s) 612
FaeI CATG 2 cut(s) 661, 898
FatI CATG 2 cut(s) 657, 894
FauNDI CATATG 2 cut(s) 246, 571
Fnu4HI GCNGC 2 cut(s) 159, 491
FokI GGATG 3 cut(s) 288, 689, 854
FriOI GRGCYC 1 cut(s) 612
Fsp4HI GCNGC 2 cut(s) 159, 491
FspBI CTAG 5 cut(s) 108, 293, 353, 755, 927
GluI GCNGC 2 cut(s) 159, 491
HaeIII GGCC 2 cut(s) 224, 763
HapII CCGG 2 cut(s) 595, 850
Hin1II CATG 2 cut(s) 661, 898
HinfI GANTC 1 cut(s) 113
HpaII CCGG 2 cut(s) 595, 850
HphI GGTGA 2 cut(s) 352, 712
Hpy166II GTNNAC 5 cut(s) 415, 577, 731, 792, 827
Hpy188I TCNGA 6 cut(s) 38, 97, 118, 624, 738, 924
Hpy188III TCNNGA 6 cut(s) 76, 86, 179, 305, 363, 850
Hpy8I GTNNAC 5 cut(s) 415, 577, 731, 792, 827
HpyAV CCTTC 1 cut(s) 80
HpyCH4III ACNGT 6 cut(s) 101, 275, 378, 422, 644, 789
HpyCH4V TGCA 7 cut(s) 161, 244, 281, 395, 565, 690, 769
HpyF10VI GCNNNNNNNGC 2 cut(s) 842, 895
HpyF3I CTNAG 4 cut(s) 190, 494, 531, 707
Hsp92II CATG 2 cut(s) 661, 898
Kpn2I TCCGGA 1 cut(s) 849
Kzo9I GATC 8 cut(s) 133, 167, 359, 511, 556, 624, 718, 852
Lsp1109I GCAGC 1 cut(s) 145
LweI GCATC 3 cut(s) 91, 778, 832
MaeI CTAG 5 cut(s) 108, 293, 353, 755, 927
MaeIII GTNAC 2 cut(s) 497, 683
MalI GATC 8 cut(s) 135, 169, 361, 513, 558, 626, 720, 854
MboI GATC 8 cut(s) 133, 167, 359, 511, 556, 624, 718, 852
MboII GAAGA 8 cut(s) 178, 212, 320, 463, 506, 638, 713, 793
MflI RGATCY 4 cut(s) 167, 556, 718, 852
MhlI GDGCHC 2 cut(s) 471, 612
MlsI TGGCCA 2 cut(s) 224, 763
MluNI TGGCCA 2 cut(s) 224, 763
MmeI TCCRAC 2 cut(s) 61, 761
MnlI CCTC 3 cut(s) 72, 403, 832
Mox20I TGGCCA 2 cut(s) 224, 763
Mph1103I ATGCAT 1 cut(s) 771
MroI TCCGGA 1 cut(s) 849
MscI TGGCCA 2 cut(s) 224, 763
MseI TTAA 2 cut(s) 140, 230
Msp20I TGGCCA 2 cut(s) 224, 763
MspI CCGG 2 cut(s) 595, 850
MspR9I CCNGG 1 cut(s) 801
Mva1269I GAATGC 1 cut(s) 532
MvaI CCWGG 1 cut(s) 801
MwoI GCNNNNNNNGC 2 cut(s) 842, 895
NdeI CATATG 2 cut(s) 246, 571
NdeII GATC 8 cut(s) 133, 167, 359, 511, 556, 624, 718, 852
NlaIII CATG 2 cut(s) 661, 898
NlaIV GGNNCC 1 cut(s) 854
NmeAIII GCCGAG 1 cut(s) 862
NmuCI GTSAC 2 cut(s) 497, 683
NsiI ATGCAT 1 cut(s) 771
NspI RCATGY 1 cut(s) 898
NspV TTCGAA 1 cut(s) 524
PctI GAATGC 1 cut(s) 532
PfeI GAWTC 1 cut(s) 113
PkrI GCNGC 2 cut(s) 160, 492
Psp124BI GAGCTC 1 cut(s) 612
Psp6I CCWGG 1 cut(s) 799
PspGI CCWGG 1 cut(s) 799
PspN4I GGNNCC 1 cut(s) 854
PspPI GGNCC 2 cut(s) 25, 592
PstI CTGCAG 1 cut(s) 397
PsuI RGATCY 4 cut(s) 167, 556, 718, 852
RsaI GTAC 1 cut(s) 793
RsaNI GTAC 1 cut(s) 792
SacI GAGCTC 1 cut(s) 612
SaqAI TTAA 2 cut(s) 140, 230
SatI GCNGC 2 cut(s) 159, 491
Sau3AI GATC 8 cut(s) 133, 167, 359, 511, 556, 624, 718, 852
Sau96I GGNCC 2 cut(s) 25, 592
ScrFI CCNGG 1 cut(s) 801
SduI GDGCHC 2 cut(s) 471, 612
SfaNI GCATC 3 cut(s) 91, 778, 832
SfcI CTRYAG 1 cut(s) 393
SfuI TTCGAA 1 cut(s) 524
SinI GGWCC 2 cut(s) 25, 592
SmlI CTYRAG 3 cut(s) 74, 428, 464
SmoI CTYRAG 3 cut(s) 74, 428, 464
SpeI ACTAGT 2 cut(s) 292, 926
SsiI CCGC 1 cut(s) 491
SspMI CTAG 5 cut(s) 108, 293, 353, 755, 927
SstI GAGCTC 1 cut(s) 612
StyD4I CCNGG 1 cut(s) 799
TaaI ACNGT 6 cut(s) 101, 275, 378, 422, 644, 789
TaqI TCGA 5 cut(s) 85, 362, 524, 543, 779
TatI WGTACW 1 cut(s) 791
TauI GCSGC 1 cut(s) 493
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 2 cut(s) 140, 230
Tru9I TTAA 2 cut(s) 140, 230
TscAI CASTG 3 cut(s) 425, 634, 794
TseFI GTSAC 2 cut(s) 497, 683
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 2 cut(s) 497, 683
TspDTI ATGAA 2 cut(s) 529, 918
TspGWI ACGGA 1 cut(s) 178
TspRI CASTG 3 cut(s) 425, 634, 794
VpaK11BI GGWCC 2 cut(s) 25, 592
XapI RAATTY 5 cut(s) 149, 536, 665, 750, 906
XceI RCATGY 1 cut(s) 898
XspI CTAG 5 cut(s) 108, 293, 353, 755, 927
Zsp2I ATGCAT 1 cut(s) 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.