MD15G1108100.v1.1

Cenp-O kinetochore centromere component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008371
Physical Location & Seq
Forward (+)
7543039 .. 7548658
5620 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1108100.v1.1.491

Sequence Viewer

Length: 1062 bp
ATGGATTACAGCGACGACGCAGAAGAGCATTTCAGGCGGAGATCGAGGAAGATGGCGTATCTCGGAGACCCATCTCGCAGGTTTGAATCGCTGAGCGGACGTTCCTATGCCCCAGGAGAAATGGGTTATATGCAAGAGGAAGATATTTCGTTAGACGCCATACGAGCTAGATGGACAAATGTTCTCAAAAGGCATAGCGAGTTAGCGGAGCGTCTTTCTAGGGATGCCGACAAGATAATTTATGAGCGTCTACAGAAGGAATTTGAAGCTGCACGGGCTATACAAACTCAAGAGACTTATTTGGATGGTGAACAATGGAACGATGGACTACTAGCAACAATAAGAGAGCGGGTTCACATGGAGGCTGAAAGAAAGGCAATGGAGGGAGACGTAAACATGTTGCCTGGTCAAGAAAAAATTACGTATAAAATCGGAAATAAGGTGATTTGCTGTTTGGAAGGGGCAAGAATTGGCATACTGTATGAGACATCTTATGCAGGAGAACCTTGTGACCTATACCATTGTGTGCTTGAGAGCAAGTCATTTCTTGAGAAGATGACAGTCCTCGAACACACCATTCCGTACTTCTTGCCAATACGAGAAGCAGAAAATGATCTTCTTTCCTCCAATGCTATGAAATTTATAGATTACATTGGAGAGATGTTACAGGCTTATGTGGACAGAAGGGAACAGGTTAGGCTTGTCAAGGAGTTATATGGGAATCAGATCAAAGAAATTCATTTTACCCTTCCCTACAGCTTAATCGTCTTTTCAAAACTTGATTTTGATTGCAAGGTGGTTGTGAAGCTTAAATATGCAGAGCTTGTTTCTGTGCTTCCAACTCATGTCAGTGTGGTAGCATGGCCAGTGTGTCAACCCAGAAAAAGCAGTCTAAATATATCATCCATGAACAAAAAGGAAAACGGAACATTGGAAGGTCCAACCACCCTCTTCTATGCAGAGGATGCTTTGCGAAATAAGAGCTTACCCGAAGCATACGCAGAAATAGTGTTGAATTTGCCCCAAACTCTTCGGGAAATATGCGAGGGGAACTCTTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CENP-O

Protein Analysis

354

Amino Acids

40.65

Weight (kDa)

5.41

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CENP-O PF09496 153 - 343 1.3e-20 Cenp-O kinetochore centromere component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 69
AccBSI CCGCTC 2 cut(s) 96, 349
AccI GTMKAC 1 cut(s) 250
AciI CCGC 4 cut(s) 37, 96, 206, 349
AcoI YGGCCR 1 cut(s) 863
AcsI RAATTY 4 cut(s) 260, 638, 735, 1015
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 1 cut(s) 584
AflIII ACRYGT 1 cut(s) 396
AgsI TTSAA 4 cut(s) 86, 266, 774, 1015
AjnI CCWGG 2 cut(s) 112, 403
AluBI AGCT 6 cut(s) 167, 269, 759, 808, 823, 984
AluI AGCT 6 cut(s) 167, 269, 759, 808, 823, 984
Alw26I GTCTC 4 cut(s) 60, 287, 381, 479
AoxI GGCC 1 cut(s) 863
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 4 cut(s) 260, 638, 735, 1015
AspS9I GGNCC 1 cut(s) 938
AsuHPI GGTGA 2 cut(s) 320, 454
AvaII GGWCC 1 cut(s) 938
BalI TGGCCA 1 cut(s) 865
BbvI GCAGC 1 cut(s) 256
BccI CCATC 5 cut(s) 46, 79, 165, 299, 317
BciT130I CCWGG 2 cut(s) 114, 405
BcoDI GTCTC 4 cut(s) 60, 287, 381, 479
BfaI CTAG 3 cut(s) 168, 219, 332
BfmI CTRYAG 2 cut(s) 251, 754
BfuAI ACCTGC 1 cut(s) 69
BisI GCNGC 1 cut(s) 270
BlpI GCTNAGC 1 cut(s) 92
BlsI GCNGC 1 cut(s) 271
Bme1390I CCNGG 2 cut(s) 114, 405
Bme18I GGWCC 1 cut(s) 938
BmgT120I GGNCC 1 cut(s) 938
BmrFI CCNGG 2 cut(s) 114, 405
BmsI GCATC 2 cut(s) 214, 955
BplI GAGNNNNNCTC 1 cut(s) 1037
Bpu1102I GCTNAGC 1 cut(s) 92
BpuEI CTTGAG 3 cut(s) 273, 551, 569
BsaAI YACGTR 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 156
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 866
Bse3DI GCAATG 1 cut(s) 384
BseBI CCWGG 2 cut(s) 114, 405
BseDI CCNNGG 1 cut(s) 112
BseGI GGATG 4 cut(s) 229, 310, 902, 970
BseMI GCAATG 1 cut(s) 384
BseMII CTCAG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 866
BseXI GCAGC 1 cut(s) 256
BsgI GTGCAG 1 cut(s) 255
BshFI GGCC 1 cut(s) 865
BsmAI GTCTC 4 cut(s) 60, 287, 381, 479
BsmBI CGTCTC 1 cut(s) 381
BsnI GGCC 1 cut(s) 865
Bso31I GGTCTC 1 cut(s) 60
Bsp143I GATC 3 cut(s) 41, 613, 726
Bsp1720I GCTNAGC 1 cut(s) 92
BspACI CCGC 4 cut(s) 37, 96, 206, 349
BspANI GGCC 1 cut(s) 865
BspCNI CTCAG 1 cut(s) 84
BspMI ACCTGC 1 cut(s) 69
BspQI GCTCTTC 1 cut(s) 18
BspTNI GGTCTC 1 cut(s) 60
BsrBI CCGCTC 2 cut(s) 96, 349
BsrDI GCAATG 1 cut(s) 384
BsrI ACTGG 1 cut(s) 866
BssECI CCNNGG 1 cut(s) 112
BssMI GATC 3 cut(s) 41, 613, 726
BssNI GRCGYC 1 cut(s) 156
Bst2UI CCWGG 2 cut(s) 114, 405
Bst4CI ACNGT 2 cut(s) 480, 562
Bst6I CTCTTC 3 cut(s) 18, 956, 1035
BstACI GRCGYC 1 cut(s) 156
BstAPI GCANNNNNTGC 1 cut(s) 965
BstBAI YACGTR 1 cut(s) 423
BstDEI CTNAG 1 cut(s) 92
BstF5I GGATG 4 cut(s) 229, 310, 902, 970
BstKTI GATC 3 cut(s) 44, 616, 729
BstMAI GTCTC 4 cut(s) 60, 287, 381, 479
BstMBI GATC 3 cut(s) 41, 613, 726
BstMWI GCNNNNNNNGC 4 cut(s) 34, 164, 275, 965
BstNI CCWGG 2 cut(s) 114, 405
BstNSI RCATGY 1 cut(s) 400
BstSCI CCNGG 2 cut(s) 112, 403
BstSFI CTRYAG 2 cut(s) 251, 754
BstSNI TACGTA 1 cut(s) 423
BstV1I GCAGC 1 cut(s) 256
BsuRI GGCC 1 cut(s) 865
BtsCI GGATG 4 cut(s) 229, 310, 902, 970
BtsIMutI CAGTG 2 cut(s) 856, 873
BveI ACCTGC 1 cut(s) 69
Cfr13I GGNCC 1 cut(s) 938
CseI GACGC 4 cut(s) 26, 164, 200, 236
Csp6I GTAC 1 cut(s) 583
CviAII CATG 5 cut(s) 358, 397, 845, 861, 907
CviQI GTAC 1 cut(s) 583
DdeI CTNAG 1 cut(s) 92
DpnI GATC 3 cut(s) 43, 615, 728
DpnII GATC 3 cut(s) 41, 613, 726
EaeI YGGCCR 1 cut(s) 863
Eam1104I CTCTTC 3 cut(s) 18, 956, 1035
EarI CTCTTC 3 cut(s) 18, 956, 1035
EciI GGCGGA 1 cut(s) 52
Eco105I TACGTA 1 cut(s) 423
Eco31I GGTCTC 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 938
EcoRII CCWGG 2 cut(s) 112, 403
Esp3I CGTCTC 1 cut(s) 381
FaeI CATG 5 cut(s) 361, 400, 848, 864, 910
FatI CATG 5 cut(s) 357, 396, 844, 860, 906
FauI CCCGC 1 cut(s) 342
FblI GTMKAC 1 cut(s) 250
Fnu4HI GCNGC 1 cut(s) 270
FokI GGATG 4 cut(s) 236, 317, 889, 977
Fsp4HI GCNGC 1 cut(s) 270
FspBI CTAG 3 cut(s) 168, 219, 332
GluI GCNGC 1 cut(s) 270
HaeIII GGCC 1 cut(s) 865
HgaI GACGC 4 cut(s) 26, 164, 200, 236
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 5 cut(s) 361, 400, 848, 864, 910
HincII GTYRAC 1 cut(s) 875
HindII GTYRAC 1 cut(s) 875
HindIII AAGCTT 1 cut(s) 806
HinfI GANTC 2 cut(s) 86, 721
HphI GGTGA 2 cut(s) 320, 454
Hpy166II GTNNAC 6 cut(s) 251, 311, 355, 394, 679, 875
Hpy188I TCNGA 3 cut(s) 65, 434, 726
Hpy188III TCNNGA 4 cut(s) 290, 410, 548, 1034
Hpy8I GTNNAC 6 cut(s) 251, 311, 355, 394, 679, 875
Hpy99I CGWCG 2 cut(s) 17, 20
HpyAV CCTTC 5 cut(s) 250, 452, 678, 758, 929
HpyCH4III ACNGT 2 cut(s) 480, 562
HpyCH4IV ACGT 3 cut(s) 100, 390, 422
HpyCH4V TGCA 6 cut(s) 133, 272, 497, 792, 818, 959
HpyF10VI GCNNNNNNNGC 4 cut(s) 34, 164, 275, 965
HpyF3I CTNAG 1 cut(s) 92
HpySE526I ACGT 3 cut(s) 100, 390, 422
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 5 cut(s) 361, 400, 848, 864, 910
Kzo9I GATC 3 cut(s) 41, 613, 726
LguI GCTCTTC 1 cut(s) 18
LmnI GCTCC 1 cut(s) 208
Lsp1109I GCAGC 1 cut(s) 256
LweI GCATC 2 cut(s) 214, 955
MaeI CTAG 3 cut(s) 168, 219, 332
MaeII ACGT 3 cut(s) 100, 390, 422
MaeIII GTNAC 2 cut(s) 509, 663
MalI GATC 3 cut(s) 43, 615, 728
MbiI CCGCTC 2 cut(s) 96, 349
MboI GATC 3 cut(s) 41, 613, 726
MboII GAAGA 8 cut(s) 35, 61, 152, 565, 608, 943, 1022, 1047
MlsI TGGCCA 1 cut(s) 865
MluCI AATT 7 cut(s) 237, 260, 417, 468, 638, 735, 1015
MluNI TGGCCA 1 cut(s) 865
MmeI TCCRAC 2 cut(s) 863, 965
MnlI CCTC 9 cut(s) 39, 130, 355, 376, 575, 634, 955, 959, 1039
Mox20I TGGCCA 1 cut(s) 865
MscI TGGCCA 1 cut(s) 865
MseI TTAA 2 cut(s) 761, 810
MslI CAYNNNNRTG 1 cut(s) 849
Msp20I TGGCCA 1 cut(s) 865
MspR9I CCNGG 2 cut(s) 114, 405
MvaI CCWGG 2 cut(s) 114, 405
MwoI GCNNNNNNNGC 4 cut(s) 34, 164, 275, 965
NdeII GATC 3 cut(s) 41, 613, 726
NlaIII CATG 5 cut(s) 361, 400, 848, 864, 910
NmuCI GTSAC 1 cut(s) 509
NspI RCATGY 1 cut(s) 400
PciI ACATGT 1 cut(s) 396
PciSI GCTCTTC 1 cut(s) 18
PfeI GAWTC 2 cut(s) 86, 721
PkrI GCNGC 1 cut(s) 271
Ppu21I YACGTR 1 cut(s) 423
PscI ACATGT 1 cut(s) 396
Psp6I CCWGG 2 cut(s) 112, 403
PspGI CCWGG 2 cut(s) 112, 403
PspPI GGNCC 1 cut(s) 938
RsaI GTAC 1 cut(s) 584
RsaNI GTAC 1 cut(s) 583
RseI CAYNNNNRTG 1 cut(s) 849
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 2 cut(s) 761, 810
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 3 cut(s) 41, 613, 726
Sau96I GGNCC 1 cut(s) 938
ScrFI CCNGG 2 cut(s) 114, 405
SfaNI GCATC 2 cut(s) 214, 955
SfcI CTRYAG 2 cut(s) 251, 754
SinI GGWCC 1 cut(s) 938
SmiMI CAYNNNNRTG 1 cut(s) 849
SmlI CTYRAG 3 cut(s) 288, 530, 548
SmoI CTYRAG 3 cut(s) 288, 530, 548
SnaBI TACGTA 1 cut(s) 423
Sse9I AATT 7 cut(s) 237, 260, 417, 468, 638, 735, 1015
SsiI CCGC 4 cut(s) 37, 96, 206, 349
SspMI CTAG 3 cut(s) 168, 219, 332
StyD4I CCNGG 2 cut(s) 112, 403
TaaI ACNGT 2 cut(s) 480, 562
TaiI ACGT 3 cut(s) 103, 393, 425
TaqI TCGA 2 cut(s) 44, 567
TasI AATT 7 cut(s) 237, 260, 417, 468, 638, 735, 1015
TfiI GAWTC 2 cut(s) 86, 721
Tru1I TTAA 2 cut(s) 761, 810
Tru9I TTAA 2 cut(s) 761, 810
TscAI CASTG 2 cut(s) 856, 873
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 1 cut(s) 269
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 3 cut(s) 650, 728, 923
TspGWI ACGGA 2 cut(s) 570, 939
TspRI CASTG 2 cut(s) 856, 873
VpaK11BI GGWCC 1 cut(s) 938
XapI RAATTY 4 cut(s) 260, 638, 735, 1015
XceI RCATGY 1 cut(s) 400
XmiI GTMKAC 1 cut(s) 250
XspI CTAG 3 cut(s) 168, 219, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.