MD08G1128700.v1.1

Cenp-O kinetochore centromere component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0008371
Physical Location & Seq
Forward (+)
12092137 .. 12103484
11348 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1128700.v1.1.491

Sequence Viewer

Length: 618 bp
ATGAGCGTCTACAGAAGGAATTTGAAGATGCACGGGCTTCACAAACTCAAGAGAATTATTTGGAAGGTGAACGATGGACTACTGGCTACAATAAGAGAGCGGGTGACTTCTTGTTTGGAAGGCGCAAGAATTGGCAAACTCTATGAGATATCTTATGCTGGAGAAACTTGTGACTTATACCATTGTGTGCTTGAGAGTAAGTCATTTCTTGAGAAGATGACAGTTCTTGAACACACCATTCCGTACTTTTTGCCGATACGAGAAGTGGAAAATGGTCTTCTTTCCTCCAATGCTATGAAACTTATAGATTACATTGGTGAGATTTTACAGGCTTATGTGGACAGAAGGGAACAAGTTGTTGTGAAGCTTAAATATGCAGAGCTTGTTCCCGTGCTTTCAAATCATGTCAGTGTGGTAGCATGGCCAGTGTGTCAACCCAGAAAAAGCAGTCCAAATATATCATCCATGAACAAAAAGGAAAACGGAACATTGGAAGGTCCAACTACCCTCTTCTATGCAGAGGATGCCTTGCGAACTATGAGCTTAACGAAAGGTCCAGCGGGGGTGTCATTCTGTGTGCTGTGGCTTCACAACCTTACTGCAAAAGTTTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CENP-O

Protein Analysis

206

Amino Acids

23.27

Weight (kDa)

9.15

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CENP-O PF09496 44 - 120 6.1e-13 Cenp-O kinetochore centromere component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 100
AccI GTMKAC 1 cut(s) 9
AciI CCGC 2 cut(s) 100, 560
AcoI YGGCCR 1 cut(s) 422
AcsI RAATTY 1 cut(s) 19
AfaI GTAC 1 cut(s) 245
AgsI TTSAA 4 cut(s) 25, 230, 399, 613
AluBI AGCT 3 cut(s) 367, 382, 543
AluI AGCT 3 cut(s) 367, 382, 543
AoxI GGCC 1 cut(s) 422
ApoI RAATTY 1 cut(s) 19
ArsI GACNNNNNNTTYG 2 cut(s) 97, 129
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 497, 554
AsuHPI GGTGA 3 cut(s) 79, 115, 329
AvaII GGWCC 2 cut(s) 497, 554
BalI TGGCCA 1 cut(s) 424
BbsI GAAGAC 1 cut(s) 269
BccI CCATC 1 cut(s) 68
BfmI CTRYAG 1 cut(s) 10
Bme18I GGWCC 2 cut(s) 497, 554
BmgT120I GGNCC 2 cut(s) 497, 554
BmsI GCATC 2 cut(s) 18, 514
BpiI GAAGAC 1 cut(s) 269
BpmI CTGGAG 1 cut(s) 180
BpuEI CTTGAG 3 cut(s) 32, 212, 230
Bse1I ACTGG 2 cut(s) 87, 425
BseGI GGATG 2 cut(s) 461, 529
BseNI ACTGG 2 cut(s) 87, 425
BshFI GGCC 1 cut(s) 424
BsnI GGCC 1 cut(s) 424
BspACI CCGC 2 cut(s) 100, 560
BspANI GGCC 1 cut(s) 424
BsrBI CCGCTC 1 cut(s) 100
BsrI ACTGG 2 cut(s) 87, 425
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 524
BstF5I GGATG 2 cut(s) 461, 529
BstHHI GCGC 1 cut(s) 125
BstMWI GCNNNNNNNGC 1 cut(s) 524
BstSFI CTRYAG 1 cut(s) 10
BstV2I GAAGAC 1 cut(s) 269
BsuRI GGCC 1 cut(s) 424
BtsCI GGATG 2 cut(s) 461, 529
BtsIMutI CAGTG 2 cut(s) 415, 432
CfoI GCGC 1 cut(s) 125
Cfr13I GGNCC 2 cut(s) 497, 554
Csp6I GTAC 1 cut(s) 244
CviAII CATG 3 cut(s) 404, 420, 466
CviJI RGCY 8 cut(s) 37, 86, 332, 367, 382, 424, 543, 586
CviKI_1 RGCY 8 cut(s) 37, 86, 332, 367, 382, 424, 543, 586
CviQI GTAC 1 cut(s) 244
EaeI YGGCCR 1 cut(s) 422
Eam1104I CTCTTC 1 cut(s) 515
EarI CTCTTC 1 cut(s) 515
Eco32I GATATC 1 cut(s) 150
Eco47I GGWCC 2 cut(s) 497, 554
EcoRV GATATC 1 cut(s) 150
FaeI CATG 3 cut(s) 407, 423, 469
FatI CATG 3 cut(s) 403, 419, 465
FauI CCCGC 2 cut(s) 93, 553
FblI GTMKAC 1 cut(s) 9
FokI GGATG 2 cut(s) 448, 536
GlaI GCGC 1 cut(s) 124
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 1 cut(s) 424
HhaI GCGC 1 cut(s) 125
Hin1II CATG 3 cut(s) 407, 423, 469
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HincII GTYRAC 1 cut(s) 434
HindII GTYRAC 1 cut(s) 434
HindIII AAGCTT 1 cut(s) 365
HphI GGTGA 3 cut(s) 79, 115, 329
Hpy166II GTNNAC 4 cut(s) 10, 70, 340, 434
Hpy188III TCNNGA 3 cut(s) 49, 209, 227
Hpy8I GTNNAC 4 cut(s) 10, 70, 340, 434
HpyAV CCTTC 5 cut(s) 9, 58, 113, 339, 488
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4V TGCA 4 cut(s) 31, 377, 518, 602
HpyF10VI GCNNNNNNNGC 1 cut(s) 524
Hsp92II CATG 3 cut(s) 407, 423, 469
HspAI GCGC 1 cut(s) 123
LpnPI CCDG 6 cut(s) 68, 144, 314, 438, 451, 570
LweI GCATC 2 cut(s) 18, 514
MaeIII GTNAC 2 cut(s) 103, 170
MbiI CCGCTC 1 cut(s) 100
MboII GAAGA 4 cut(s) 37, 226, 269, 502
MlsI TGGCCA 1 cut(s) 424
MluCI AATT 3 cut(s) 19, 54, 129
MluNI TGGCCA 1 cut(s) 424
MmeI TCCRAC 1 cut(s) 524
MnlI CCTC 3 cut(s) 295, 514, 518
Mox20I TGGCCA 1 cut(s) 424
MscI TGGCCA 1 cut(s) 424
MseI TTAA 2 cut(s) 369, 545
MslI CAYNNNNRTG 1 cut(s) 408
Msp20I TGGCCA 1 cut(s) 424
MspA1I CMGCKG 1 cut(s) 560
MwoI GCNNNNNNNGC 1 cut(s) 524
NlaIII CATG 3 cut(s) 407, 423, 469
NmuCI GTSAC 2 cut(s) 103, 170
PspPI GGNCC 2 cut(s) 497, 554
RsaI GTAC 1 cut(s) 245
RsaNI GTAC 1 cut(s) 244
RseI CAYNNNNRTG 1 cut(s) 408
SaqAI TTAA 2 cut(s) 369, 545
Sau96I GGNCC 2 cut(s) 497, 554
SetI ASST 7 cut(s) 69, 369, 384, 499, 545, 556, 597
SfaNI GCATC 2 cut(s) 18, 514
SfcI CTRYAG 1 cut(s) 10
SinI GGWCC 2 cut(s) 497, 554
SmiMI CAYNNNNRTG 1 cut(s) 408
SmlI CTYRAG 3 cut(s) 47, 191, 209
SmoI CTYRAG 3 cut(s) 47, 191, 209
Sse9I AATT 3 cut(s) 19, 54, 129
SsiI CCGC 2 cut(s) 100, 560
TaaI ACNGT 1 cut(s) 223
TasI AATT 3 cut(s) 19, 54, 129
Tru1I TTAA 2 cut(s) 369, 545
Tru9I TTAA 2 cut(s) 369, 545
TscAI CASTG 2 cut(s) 415, 432
TseFI GTSAC 2 cut(s) 103, 170
Tsp45I GTSAC 2 cut(s) 103, 170
TspDTI ATGAA 2 cut(s) 311, 482
TspGWI ACGGA 2 cut(s) 231, 498
TspRI CASTG 2 cut(s) 415, 432
VpaK11BI GGWCC 2 cut(s) 497, 554
XapI RAATTY 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.