Rroxscaffold_7G00163320

Cenp-O kinetochore centromere component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0008371
Physical Location & Seq
Forward (+)
5602273 .. 5607811
5539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00163320.1

Sequence Viewer

Length: 987 bp
ATGGATTACAGCGACACAGACGATTCGCTGCGCCGGAGGTCCAGGAAAATCGCTTATCTCGGCGAATCCGCTCGCAGGCAATTTGAATCGCCAAGTGGACGTTCCTATGGATTAATGGGAGAATTGAACTATATGCAGGAGGAAGATATTCGGCTAGATGCGACACGATCTAGATTGTCGAATGCTTTGAAGAGGCATGGTGATTTAGCCGAGCGTATGTCTAGGGATTCTGACAAGACAATTTACGAGCGTCTACATAAGGAATTTGAAGCTGCACGGGCTTTGCAAACTCAAGAGATTTATTTAGATGGCGAACAATGGAATGATGGACTGCTAGCCACAATAAGAGAACGGGTTCACATGGAGGTAGACAGAAAGGCGATGGCGAGAGATGTAAGCACGTTGCACAGTCCTCATTTCCAAGAGAGAATCACTTACAAAGTTGGGGATAAGGTGATATGTTGTTTGGAAGGAGCAAGAATTGGCATACTGTATGAGACATCTTATGCGGGAGAACCTTGTGATATATACCATTTAGTACTTGAGAGCAAGTCATTTCTAGAGAAGATGACCGTCCTTGAACACACCATTCCATTTTTTTTGCCACTAAGAGAAGCGGAAAATGATCTTCTTTCCTCCAATGCTATGAAATTTATAGATCATGTTGGAGAGCTTTTGCAGGCTTATGTGGACAGAAAGGAACAGGTTCGGCTCGTCAAGGAGTTATATGGGAACCAGATTAAAGAAATTCACTGTAGCCTGACCTACCATTTGATTTTGTTTGCAATTGCTGATTTTGGCTGCAAGGTAATTGTTAAGCTTAAATATGCAGATCTTGTTTCCGTACTTCCAACTAATGTCAGTGTGGTAGCATGGCCCATTCAAATCCGGAGATTCAGTCTAAATACATCAACCATGAATACACTGGAAGGTCCAACTTGTCTCCTCTATGCAGAGGATGCCTTGCAGAACTATGAGCTTACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CENP-O

Protein Analysis

328

Amino Acids

37.85

Weight (kDa)

5.75

Isoelectric Point (pI)

42.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CENP-O PF09496 145 - 279 1.2e-21 Cenp-O kinetochore centromere component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 71
AccI GTMKAC 2 cut(s) 253, 369
AccIII TCCGGA 1 cut(s) 888
AciI CCGC 3 cut(s) 69, 509, 617
AcsI RAATTY 3 cut(s) 263, 650, 747
AfaI GTAC 2 cut(s) 540, 846
AfiI CCNNNNNNNGG 1 cut(s) 75
AgsI TTSAA 6 cut(s) 86, 127, 190, 269, 581, 884
AjnI CCWGG 1 cut(s) 41
AjuI GAANNNNNNNTTGG 2 cut(s) 85, 117
AluBI AGCT 4 cut(s) 272, 673, 820, 979
AluI AGCT 4 cut(s) 272, 673, 820, 979
Alw26I GTCTC 2 cut(s) 491, 947
Aor13HI TCCGGA 1 cut(s) 888
AoxI GGCC 1 cut(s) 875
ApeKI GCWGC 3 cut(s) 28, 272, 801
ApoI RAATTY 3 cut(s) 263, 650, 747
ArsI GACNNNNNNTTYG 2 cut(s) 754, 786
AseI ATTAAT 1 cut(s) 113
Asp700I GAANNNNTTC 3 cut(s) 147, 354, 705
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 3 cut(s) 39, 876, 932
AsuHPI GGTGA 2 cut(s) 212, 466
AsuNHI GCTAGC 1 cut(s) 334
AvaII GGWCC 2 cut(s) 39, 932
BbvI GCAGC 3 cut(s) 15, 259, 788
BccI CCATC 3 cut(s) 302, 320, 376
BciT130I CCWGG 1 cut(s) 43
BcoDI GTCTC 2 cut(s) 491, 947
BfaI CTAG 5 cut(s) 155, 171, 222, 335, 560
BfmI CTRYAG 1 cut(s) 754
BglII AGATCT 1 cut(s) 832
BisI GCNGC 3 cut(s) 29, 273, 802
BlsI GCNGC 3 cut(s) 30, 274, 803
BmcAI AGTACT 1 cut(s) 540
Bme1390I CCNGG 1 cut(s) 43
Bme18I GGWCC 2 cut(s) 39, 932
BmgT120I GGNCC 3 cut(s) 39, 876, 932
BmiI GGNNCC 1 cut(s) 734
BmrFI CCNGG 1 cut(s) 43
BmsI GCATC 2 cut(s) 148, 949
BmtI GCTAGC 1 cut(s) 338
BpuEI CTTGAG 2 cut(s) 276, 563
BsaWI WCCGGW 1 cut(s) 888
BsaXI ACNNNNNCTCC 2 cut(s) 883, 913
Bsc4I CCNNNNNNNGG 1 cut(s) 75
Bse1I ACTGG 1 cut(s) 930
BseAI TCCGGA 1 cut(s) 888
BseBI CCWGG 1 cut(s) 43
BseGI GGATG 1 cut(s) 964
BseLI CCNNNNNNNGG 1 cut(s) 75
BseNI ACTGG 1 cut(s) 930
BseRI GAGGAG 1 cut(s) 935
BseXI GCAGC 3 cut(s) 15, 259, 788
BsgI GTGCAG 1 cut(s) 258
BshFI GGCC 1 cut(s) 877
BsiSI CCGG 2 cut(s) 34, 889
BslI CCNNNNNNNGG 1 cut(s) 75
BsmAI GTCTC 2 cut(s) 491, 947
BsmI GAATGC 1 cut(s) 187
BsnI GGCC 1 cut(s) 877
Bsp13I TCCGGA 1 cut(s) 888
Bsp143I GATC 4 cut(s) 167, 625, 658, 832
BspACI CCGC 3 cut(s) 69, 509, 617
BspANI GGCC 1 cut(s) 877
BspEI TCCGGA 1 cut(s) 888
BspLI GGNNCC 1 cut(s) 734
BspOI GCTAGC 1 cut(s) 338
BsrBI CCGCTC 1 cut(s) 71
BsrI ACTGG 1 cut(s) 930
BssMI GATC 4 cut(s) 167, 625, 658, 832
Bst2UI CCWGG 1 cut(s) 43
Bst4CI ACNGT 4 cut(s) 410, 492, 574, 755
Bst6I CTCTTC 1 cut(s) 185
BstAPI GCANNNNNTGC 1 cut(s) 959
BstC8I GCNNGC 4 cut(s) 73, 77, 336, 681
BstDEI CTNAG 1 cut(s) 608
BstF5I GGATG 1 cut(s) 964
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 4 cut(s) 170, 628, 661, 835
BstMAI GTCTC 2 cut(s) 491, 947
BstMBI GATC 4 cut(s) 167, 625, 658, 832
BstMWI GCNNNNNNNGC 2 cut(s) 278, 959
BstNI CCWGG 1 cut(s) 43
BstSCI CCNGG 1 cut(s) 41
BstSFI CTRYAG 1 cut(s) 754
BstV1I GCAGC 3 cut(s) 15, 259, 788
BstX2I RGATCY 1 cut(s) 832
BstYI RGATCY 1 cut(s) 832
BsuRI GGCC 1 cut(s) 877
BtgZI GCGATG 1 cut(s) 395
BtsCI GGATG 1 cut(s) 964
BtsIMutI CAGTG 3 cut(s) 751, 868, 923
Cac8I GCNNGC 4 cut(s) 73, 77, 336, 681
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 3 cut(s) 39, 876, 932
CseI GACGC 1 cut(s) 239
Csp6I GTAC 2 cut(s) 539, 845
CviAII CATG 5 cut(s) 197, 361, 662, 873, 916
CviQI GTAC 2 cut(s) 539, 845
DdeI CTNAG 1 cut(s) 608
DpnI GATC 4 cut(s) 169, 627, 660, 834
DpnII GATC 4 cut(s) 167, 625, 658, 832
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Eco47I GGWCC 2 cut(s) 39, 932
EcoRII CCWGG 1 cut(s) 41
FaeI CATG 5 cut(s) 200, 364, 665, 876, 919
FatI CATG 5 cut(s) 196, 360, 661, 872, 915
FauI CCCGC 1 cut(s) 502
FblI GTMKAC 2 cut(s) 253, 369
Fnu4HI GCNGC 3 cut(s) 29, 273, 802
FokI GGATG 1 cut(s) 971
Fsp4HI GCNGC 3 cut(s) 29, 273, 802
FspBI CTAG 5 cut(s) 155, 171, 222, 335, 560
GlaI GCGC 1 cut(s) 32
GluI GCNGC 3 cut(s) 29, 273, 802
HaeIII GGCC 1 cut(s) 877
HapII CCGG 2 cut(s) 34, 889
HgaI GACGC 1 cut(s) 239
HhaI GCGC 1 cut(s) 33
Hin1II CATG 5 cut(s) 200, 364, 665, 876, 919
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HindIII AAGCTT 1 cut(s) 818
HinfI GANTC 6 cut(s) 23, 65, 86, 227, 429, 894
HpaII CCGG 2 cut(s) 34, 889
HphI GGTGA 2 cut(s) 212, 466
Hpy166II GTNNAC 5 cut(s) 98, 254, 358, 370, 691
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 4 cut(s) 171, 293, 560, 889
Hpy8I GTNNAC 5 cut(s) 98, 254, 358, 370, 691
HpyAV CCTTC 2 cut(s) 464, 923
HpyCH4III ACNGT 4 cut(s) 410, 492, 574, 755
HpyCH4IV ACGT 2 cut(s) 100, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 278, 959
HpyF3I CTNAG 1 cut(s) 608
HpySE526I ACGT 2 cut(s) 100, 401
Hsp92II CATG 5 cut(s) 200, 364, 665, 876, 919
HspAI GCGC 1 cut(s) 31
Kpn2I TCCGGA 1 cut(s) 888
Kzo9I GATC 4 cut(s) 167, 625, 658, 832
LmnI GCTCC 1 cut(s) 473
Lsp1109I GCAGC 3 cut(s) 15, 259, 788
LweI GCATC 2 cut(s) 148, 949
MaeI CTAG 5 cut(s) 155, 171, 222, 335, 560
MaeII ACGT 2 cut(s) 100, 401
MalI GATC 4 cut(s) 169, 627, 660, 834
MbiI CCGCTC 1 cut(s) 71
MboI GATC 4 cut(s) 167, 625, 658, 832
MboII GAAGA 4 cut(s) 155, 202, 577, 620
MfeI CAATTG 1 cut(s) 786
MflI RGATCY 1 cut(s) 832
MluCI AATT 9 cut(s) 80, 122, 240, 263, 480, 650, 747, 786, 810
MmeI TCCRAC 3 cut(s) 646, 875, 959
MnlI CCTC 8 cut(s) 30, 133, 186, 358, 423, 646, 949, 956
MroI TCCGGA 1 cut(s) 888
MroXI GAANNNNTTC 3 cut(s) 147, 354, 705
MseI TTAA 4 cut(s) 113, 741, 816, 822
MspI CCGG 2 cut(s) 34, 889
MspR9I CCNGG 1 cut(s) 43
MunI CAATTG 1 cut(s) 786
Mva1269I GAATGC 1 cut(s) 187
MvaI CCWGG 1 cut(s) 43
MwoI GCNNNNNNNGC 2 cut(s) 278, 959
NdeII GATC 4 cut(s) 167, 625, 658, 832
NheI GCTAGC 1 cut(s) 334
NlaIII CATG 5 cut(s) 200, 364, 665, 876, 919
NlaIV GGNNCC 1 cut(s) 734
NmeAIII GCCGAG 2 cut(s) 39, 235
PctI GAATGC 1 cut(s) 187
PdmI GAANNNNTTC 3 cut(s) 147, 354, 705
PfeI GAWTC 6 cut(s) 23, 65, 86, 227, 429, 894
PfoI TCCNGGA 1 cut(s) 41
PkrI GCNGC 3 cut(s) 30, 274, 803
PshBI ATTAAT 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 41
PspGI CCWGG 1 cut(s) 41
PspN4I GGNNCC 1 cut(s) 734
PspPI GGNCC 3 cut(s) 39, 876, 932
PsuI RGATCY 1 cut(s) 832
RsaI GTAC 2 cut(s) 540, 846
RsaNI GTAC 2 cut(s) 539, 845
SaqAI TTAA 4 cut(s) 113, 741, 816, 822
SatI GCNGC 3 cut(s) 29, 273, 802
Sau3AI GATC 4 cut(s) 167, 625, 658, 832
Sau96I GGNCC 3 cut(s) 39, 876, 932
ScaI AGTACT 1 cut(s) 540
ScrFI CCNGG 1 cut(s) 43
SfaNI GCATC 2 cut(s) 148, 949
SfcI CTRYAG 1 cut(s) 754
SinI GGWCC 2 cut(s) 39, 932
SmlI CTYRAG 2 cut(s) 291, 542
SmoI CTYRAG 2 cut(s) 291, 542
Sse9I AATT 9 cut(s) 80, 122, 240, 263, 480, 650, 747, 786, 810
SsiI CCGC 3 cut(s) 69, 509, 617
SspMI CTAG 5 cut(s) 155, 171, 222, 335, 560
StyD4I CCNGG 1 cut(s) 41
TaaI ACNGT 4 cut(s) 410, 492, 574, 755
TaiI ACGT 2 cut(s) 103, 404
TaqI TCGA 1 cut(s) 179
TasI AATT 9 cut(s) 80, 122, 240, 263, 480, 650, 747, 786, 810
TatI WGTACW 1 cut(s) 538
TfiI GAWTC 6 cut(s) 23, 65, 86, 227, 429, 894
Tru1I TTAA 4 cut(s) 113, 741, 816, 822
Tru9I TTAA 4 cut(s) 113, 741, 816, 822
TscAI CASTG 3 cut(s) 758, 868, 930
TseI GCWGC 3 cut(s) 28, 272, 801
TspDTI ATGAA 2 cut(s) 662, 932
TspGWI ACGGA 1 cut(s) 832
TspRI CASTG 3 cut(s) 758, 868, 930
VpaK11BI GGWCC 2 cut(s) 39, 932
VspI ATTAAT 1 cut(s) 113
XapI RAATTY 3 cut(s) 263, 650, 747
XbaI TCTAGA 2 cut(s) 170, 559
XcmI CCANNNNNNNNNTGG 1 cut(s) 922
XmiI GTMKAC 2 cut(s) 253, 369
XmnI GAANNNNTTC 3 cut(s) 147, 354, 705
XspI CTAG 5 cut(s) 155, 171, 222, 335, 560
ZrmI AGTACT 1 cut(s) 540
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.