AT5G11650

Serine aminopeptidase, S33

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013002
Physical Location & Seq
Forward (+)
3744698 .. 3747198
2501 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G11650.1

Sequence Viewer

Length: 1173 bp
ATGGCGTCAACGTCGGCGGAAATGGACCAATTAACATCAGGCGCGAGCAATCGGATAATATTTATCTTGAGAACGCTTAGGAAGTGTCTCGTCTTCGTTCTCTCTCTCGTCCTTTCTCTTCTCCTTATTCTTCGTCTCCGTCCTCGTCGCCGTGTCTCTCCTCTTTCATCGCCTGAGGATGAGGCTGTTCCGGCTCCTTCGAGGCGATGGAGACGGAAGATGGCGTGGAAGCTGGAGGAGGAAGATACGGCTAGACGTCGTTCCTTAGCGGAAGGCGTTGAGATGGCCGGAGATGGAGAGATCTCTTGTTCGTTGTTTTACGGACGTCGTGGTAACGCTTTATTCTCTCGGTCGTGGTTGCCTATTTCCGGCGAACTTAGGGGGATTTTGATTATTATCCATGGATTGAATGAACACAGTGGAAGATATTCTCAATTCGCTAAGCAGCTAAACGCGAGTAATTTAGGTGTATACGCAATGGACTGGATTGGTCACGGTGGGAGTGATGGTTTGCATGGTTATGTTCCTTCTCTTGATTATGTTGTTTCCGACACTGAAGCTTTCTTGGAAAAGATTAGGTCTGAGAATCCGGGGGTTCCGTGTTTCCTCTTTGGTCACTCTACCGGTGGAGCTGTGGTTTTGAAGGCAGCTTCATCTCCCTCTATTGAAGATATGTTAGCCGGTATTGTCCTAACATCGCCTGCACTCCGTGTCAAACCCGCTCATCCCATTGTCGGGGCAATAGCTCCAATCTTCTCATTGCTCGCACCAAGGTTCCAATTCAAAGGAGCAAACAAGAGAGGCATTCCGGTCTCAAGAGACCCTGAAGCTCTCTTAGCCAAGTACTCTGATCCGTTAGTCTACACTGGTCCGATTAGAGTCCGAACAGGGTATGAGATTCTTCGCATAACGGCTTACTTGACCCGGAACTTCAAATCTGTTACAGTGCCTTTCTTTGTTCTCCATGGTACAGAGGACAAAGTCACTGATCCACTAGCTTCCCAAGACTTGTATAATCAGGCACCATCAGTTTTCAAAGACATCAAACTCTATGACGGTTTCTTACACGACCTTCTCTTTGAACCTGAGCGTGAGGAAGTCGGTCGTGATATCATAGATTGGATGATGAATCGGCTTGACGATGTTAACGGATCAGCAGCTAGCCTCTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

390

Amino Acids

43.27

Weight (kDa)

8.66

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 125 - 363 8.3e-74 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 129 - 343 5.5e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 130 - 338 4.1e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013002)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11650 AT5G11650
fragaria_vesca FvH4_2g35400
malus_domestica MD08G1112500.v1.1 MD15G1091700.v1.1
prunus_persica Prupe.1G446700_v2.0.a1
pyrus_communis pycom08g09340 pycom15g08550
rosa_chinensis RchiOBHm_Chr6g0306501
rosa_laevigata RLG00000010791
rosa_multiflora Rmu_sc0002971.1_g000010
rosa_roxburghii Rroxscaffold_7G00161720
rosa_rugosa Rorug06G0350000
rosa_samantha Rh6AG462100 Rh6BG438700 Rh6CG476400 Rh6DG463100
rosa_wichuraiana Rw6G040280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 259, 328
AccB1I GGYRCC 1 cut(s) 1021
AccBSI CCGCTC 1 cut(s) 722
AccI GTMKAC 2 cut(s) 471, 861
AccII CGCG 2 cut(s) 44, 455
AciI CCGC 3 cut(s) 17, 269, 720
AclWI GGATC 3 cut(s) 845, 983, 1159
AcoI YGGCCR 1 cut(s) 285
AcuI CTGAAG 2 cut(s) 576, 846
AcyI GRCGYC 3 cut(s) 5, 256, 325
AdeI CACNNNGTG 1 cut(s) 710
AfaI GTAC 2 cut(s) 845, 970
AfiI CCNNNNNNNGG 3 cut(s) 368, 734, 735
AgeI ACCGGT 1 cut(s) 623
AgsI TTSAA 7 cut(s) 409, 643, 668, 784, 934, 1036, 1082
AluBI AGCT 9 cut(s) 232, 448, 560, 632, 650, 746, 830, 998, 1160
AluI AGCT 9 cut(s) 232, 448, 560, 632, 650, 746, 830, 998, 1160
Alw26I GTCTC 6 cut(s) 92, 140, 160, 205, 813, 817
AlwI GGATC 3 cut(s) 845, 983, 1159
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 3 cut(s) 445, 647, 1157
ArsI GACNNNNNNTTYG 2 cut(s) 1061, 1093
AsiGI ACCGGT 1 cut(s) 623
Asp700I GAANNNNTTC 1 cut(s) 427
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 2 cut(s) 25, 869
AsuC2I CCSGG 2 cut(s) 591, 925
AsuNHI GCTAGC 1 cut(s) 1160
AvaII GGWCC 2 cut(s) 25, 869
AxyI CCTNAGG 1 cut(s) 174
BanI GGYRCC 1 cut(s) 1021
BbsI GAAGAC 1 cut(s) 85
BbvI GCAGC 3 cut(s) 457, 659, 1169
BccI CCATC 6 cut(s) 201, 214, 277, 287, 500, 1033
BceAI ACGGC 3 cut(s) 135, 264, 927
BcnI CCSGG 2 cut(s) 591, 925
BcoDI GTCTC 6 cut(s) 92, 140, 160, 205, 813, 817
BfaI CTAG 3 cut(s) 252, 995, 1161
BglII AGATCT 1 cut(s) 300
BisI GCNGC 3 cut(s) 446, 648, 1158
BlpI GCTNAGC 1 cut(s) 441
BlsI GCNGC 3 cut(s) 447, 649, 1159
BmcAI AGTACT 1 cut(s) 845
Bme1390I CCNGG 2 cut(s) 591, 925
Bme18I GGWCC 2 cut(s) 25, 869
BmgT120I GGNCC 2 cut(s) 25, 869
BmiI GGNNCC 4 cut(s) 195, 597, 776, 1023
BmrFI CCNGG 2 cut(s) 591, 925
BmtI GCTAGC 1 cut(s) 1164
BpiI GAAGAC 1 cut(s) 85
BpmI CTGGAG 1 cut(s) 254
Bpu10I CCTNAGC 3 cut(s) 77, 265, 1086
Bpu1102I GCTNAGC 1 cut(s) 441
BpuEI CTTGAG 2 cut(s) 88, 799
BpuMI CCSGG 2 cut(s) 591, 925
BsaBI GATNNNNATC 1 cut(s) 395
BsaHI GRCGYC 3 cut(s) 5, 256, 325
BsaI GGTCTC 2 cut(s) 813, 817
BsaJI CCNNGG 4 cut(s) 400, 590, 770, 964
BsaWI WCCGGW 2 cut(s) 623, 808
BsaXI ACNNNNNCTCC 4 cut(s) 493, 523, 621, 651
Bsc4I CCNNNNNNNGG 3 cut(s) 368, 734, 735
Bse118I RCCGGY 2 cut(s) 623, 680
Bse1I ACTGG 2 cut(s) 488, 871
Bse21I CCTNAGG 1 cut(s) 174
Bse3DI GCAATG 2 cut(s) 483, 758
Bse8I GATNNNNATC 1 cut(s) 395
BseDI CCNNGG 4 cut(s) 400, 590, 770, 964
BseGI GGATG 3 cut(s) 184, 724, 1128
BseJI GATNNNNATC 1 cut(s) 395
BseLI CCNNNNNNNGG 3 cut(s) 368, 734, 735
BseMI GCAATG 2 cut(s) 483, 758
BseMII CTCAG 3 cut(s) 165, 573, 1077
BseNI ACTGG 2 cut(s) 488, 871
BseRI GAGGAG 2 cut(s) 150, 251
BseXI GCAGC 3 cut(s) 457, 659, 1169
BsgI GTGCAG 1 cut(s) 687
Bsh1236I CGCG 2 cut(s) 44, 455
Bsh1285I CGRYCG 2 cut(s) 353, 1105
BshFI GGCC 1 cut(s) 287
BshNI GGYRCC 1 cut(s) 1021
BshTI ACCGGT 1 cut(s) 623
BsiEI CGRYCG 2 cut(s) 353, 1105
BsiSI CCGG 8 cut(s) 191, 288, 369, 590, 624, 681, 809, 925
BslI CCNNNNNNNGG 3 cut(s) 368, 734, 735
BsmAI GTCTC 6 cut(s) 92, 140, 160, 205, 813, 817
BsmBI CGTCTC 2 cut(s) 140, 205
BsmI GAATGC 1 cut(s) 804
BsnI GGCC 1 cut(s) 287
Bso31I GGTCTC 2 cut(s) 813, 817
Bsp143I GATC 4 cut(s) 300, 850, 988, 1151
Bsp1720I GCTNAGC 1 cut(s) 441
Bsp19I CCATGG 2 cut(s) 400, 964
BspACI CCGC 3 cut(s) 17, 269, 720
BspANI GGCC 1 cut(s) 287
BspCNI CTCAG 3 cut(s) 166, 574, 1078
BspFNI CGCG 2 cut(s) 44, 455
BspLI GGNNCC 4 cut(s) 195, 597, 776, 1023
BspOI GCTAGC 1 cut(s) 1164
BspPI GGATC 3 cut(s) 845, 983, 1159
BspT107I GGYRCC 1 cut(s) 1021
BspTNI GGTCTC 2 cut(s) 813, 817
BsrBI CCGCTC 1 cut(s) 722
BsrDI GCAATG 2 cut(s) 483, 758
BsrFI RCCGGY 2 cut(s) 623, 680
BsrI ACTGG 2 cut(s) 488, 871
BssAI RCCGGY 2 cut(s) 623, 680
BssECI CCNNGG 4 cut(s) 400, 590, 770, 964
BssMI GATC 4 cut(s) 300, 850, 988, 1151
BssNAI GTATAC 1 cut(s) 472
BssNI GRCGYC 3 cut(s) 5, 256, 325
BssT1I CCWWGG 3 cut(s) 400, 770, 964
Bst1107I GTATAC 1 cut(s) 472
Bst4CI ACNGT 4 cut(s) 419, 497, 946, 1058
Bst6I CTCTTC 1 cut(s) 123
BstACI GRCGYC 3 cut(s) 5, 256, 325
BstC8I GCNNGC 4 cut(s) 46, 702, 765, 1162
BstDEI CTNAG 8 cut(s) 77, 174, 265, 377, 441, 582, 835, 1086
BstDSI CCRYGG 2 cut(s) 400, 964
BstF5I GGATG 3 cut(s) 184, 724, 1128
BstFNI CGCG 2 cut(s) 44, 455
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 4 cut(s) 303, 853, 991, 1154
BstMAI GTCTC 6 cut(s) 92, 140, 160, 205, 813, 817
BstMBI GATC 4 cut(s) 300, 850, 988, 1151
BstMCI CGRYCG 2 cut(s) 353, 1105
BstMWI GCNNNNNNNGC 2 cut(s) 191, 836
BstSCI CCNGG 2 cut(s) 589, 923
BstUI CGCG 2 cut(s) 44, 455
BstV1I GCAGC 3 cut(s) 457, 659, 1169
BstV2I GAAGAC 1 cut(s) 85
BstX2I RGATCY 1 cut(s) 300
BstYI RGATCY 1 cut(s) 300
BstZ17I GTATAC 1 cut(s) 472
Bsu36I CCTNAGG 1 cut(s) 174
BsuRI GGCC 1 cut(s) 287
BtgI CCRYGG 2 cut(s) 400, 964
BtgZI GCGATG 3 cut(s) 153, 220, 681
BtsCI GGATG 3 cut(s) 184, 724, 1128
BtsIMutI CAGTG 5 cut(s) 424, 552, 864, 951, 984
Cac8I GCNNGC 4 cut(s) 46, 702, 765, 1162
CfoI GCGC 1 cut(s) 44
Cfr10I RCCGGY 2 cut(s) 623, 680
Cfr13I GGNCC 2 cut(s) 25, 869
Csp6I GTAC 2 cut(s) 844, 969
CspAI ACCGGT 1 cut(s) 623
CviAII CATG 3 cut(s) 401, 515, 965
CviQI GTAC 2 cut(s) 844, 969
DdeI CTNAG 8 cut(s) 77, 174, 265, 377, 441, 582, 835, 1086
DpnI GATC 4 cut(s) 302, 852, 990, 1153
DpnII GATC 4 cut(s) 300, 850, 988, 1151
DraIII CACNNNGTG 1 cut(s) 710
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 123
EarI CTCTTC 1 cut(s) 123
EciI GGCGGA 1 cut(s) 32
Eco130I CCWWGG 3 cut(s) 400, 770, 964
Eco31I GGTCTC 2 cut(s) 813, 817
Eco32I GATATC 1 cut(s) 1111
Eco47I GGWCC 2 cut(s) 25, 869
Eco57I CTGAAG 2 cut(s) 576, 846
Eco81I CCTNAGG 1 cut(s) 174
EcoRV GATATC 1 cut(s) 1111
EcoT14I CCWWGG 3 cut(s) 400, 770, 964
ErhI CCWWGG 3 cut(s) 400, 770, 964
Esp3I CGTCTC 2 cut(s) 140, 205
FaeI CATG 3 cut(s) 404, 518, 968
FatI CATG 3 cut(s) 400, 514, 964
FauI CCCGC 1 cut(s) 727
FblI GTMKAC 2 cut(s) 471, 861
Fnu4HI GCNGC 3 cut(s) 446, 648, 1158
FokI GGATG 3 cut(s) 191, 711, 1135
Fsp4HI GCNGC 3 cut(s) 446, 648, 1158
FspBI CTAG 3 cut(s) 252, 995, 1161
GlaI GCGC 1 cut(s) 43
GluI GCNGC 3 cut(s) 446, 648, 1158
GsuI CTGGAG 1 cut(s) 254
HaeIII GGCC 1 cut(s) 287
HapII CCGG 8 cut(s) 191, 288, 369, 590, 624, 681, 809, 925
HhaI GCGC 1 cut(s) 44
Hin1I GRCGYC 3 cut(s) 5, 256, 325
Hin1II CATG 3 cut(s) 404, 518, 968
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HincII GTYRAC 2 cut(s) 9, 1147
HindII GTYRAC 2 cut(s) 9, 1147
HindIII AAGCTT 1 cut(s) 558
HinfI GANTC 4 cut(s) 586, 879, 898, 1129
HpaI GTTAAC 1 cut(s) 1147
HpaII CCGG 8 cut(s) 191, 288, 369, 590, 624, 681, 809, 925
Hpy166II GTNNAC 4 cut(s) 9, 472, 862, 1147
Hpy188I TCNGA 6 cut(s) 54, 550, 583, 850, 873, 884
Hpy188III TCNNGA 4 cut(s) 67, 533, 816, 1106
Hpy8I GTNNAC 4 cut(s) 9, 472, 862, 1147
Hpy99I CGWCG 4 cut(s) 16, 150, 261, 330
HpyAV CCTTC 5 cut(s) 207, 266, 537, 637, 1082
HpyCH4III ACNGT 4 cut(s) 419, 497, 946, 1058
HpyCH4IV ACGT 3 cut(s) 11, 256, 325
HpyCH4V TGCA 2 cut(s) 514, 704
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 836
HpyF3I CTNAG 8 cut(s) 77, 174, 265, 377, 441, 582, 835, 1086
HpySE526I ACGT 3 cut(s) 11, 256, 325
Hsp92I GRCGYC 3 cut(s) 5, 256, 325
Hsp92II CATG 3 cut(s) 404, 518, 968
HspAI GCGC 1 cut(s) 42
KspAI GTTAAC 1 cut(s) 1147
Kzo9I GATC 4 cut(s) 300, 850, 988, 1151
LmnI GCTCC 4 cut(s) 199, 629, 751, 788
Lsp1109I GCAGC 3 cut(s) 457, 659, 1169
MaeI CTAG 3 cut(s) 252, 995, 1161
MaeII ACGT 3 cut(s) 11, 256, 325
MaeIII GTNAC 5 cut(s) 332, 491, 614, 940, 982
MalI GATC 4 cut(s) 302, 852, 990, 1153
MbiI CCGCTC 1 cut(s) 722
MboI GATC 4 cut(s) 300, 850, 988, 1151
MboII GAAGA 9 cut(s) 85, 110, 122, 229, 254, 435, 680, 745, 893
MflI RGATCY 1 cut(s) 300
MluCI AATT 4 cut(s) 29, 434, 460, 779
MlyI GAGTC 1 cut(s) 888
MmeI TCCRAC 1 cut(s) 573
MroXI GAANNNNTTC 1 cut(s) 427
MseI TTAA 2 cut(s) 32, 1146
MslI CAYNNNNRTG 1 cut(s) 519
MspI CCGG 8 cut(s) 191, 288, 369, 590, 624, 681, 809, 925
MspR9I CCNGG 2 cut(s) 591, 925
Mva1269I GAATGC 1 cut(s) 804
MvnI CGCG 2 cut(s) 44, 455
MwoI GCNNNNNNNGC 2 cut(s) 191, 836
NciI CCSGG 2 cut(s) 591, 925
NcoI CCATGG 2 cut(s) 400, 964
NdeII GATC 4 cut(s) 300, 850, 988, 1151
NheI GCTAGC 1 cut(s) 1160
NlaIII CATG 3 cut(s) 404, 518, 968
NlaIV GGNNCC 4 cut(s) 195, 597, 776, 1023
NmuCI GTSAC 3 cut(s) 491, 614, 982
PctI GAATGC 1 cut(s) 804
PdmI GAANNNNTTC 1 cut(s) 427
PfeI GAWTC 3 cut(s) 586, 898, 1129
PflFI GACNNNGTC 1 cut(s) 980
PinAI ACCGGT 1 cut(s) 623
PkrI GCNGC 3 cut(s) 447, 649, 1159
PleI GAGTC 1 cut(s) 887
PpsI GAGTC 1 cut(s) 887
PspN4I GGNNCC 4 cut(s) 195, 597, 776, 1023
PspPI GGNCC 2 cut(s) 25, 869
PsuI RGATCY 1 cut(s) 300
PsyI GACNNNGTC 1 cut(s) 980
RsaI GTAC 2 cut(s) 845, 970
RsaNI GTAC 2 cut(s) 844, 969
RseI CAYNNNNRTG 1 cut(s) 519
SaqAI TTAA 2 cut(s) 32, 1146
SatI GCNGC 3 cut(s) 446, 648, 1158
Sau3AI GATC 4 cut(s) 300, 850, 988, 1151
Sau96I GGNCC 2 cut(s) 25, 869
ScaI AGTACT 1 cut(s) 845
SchI GAGTC 1 cut(s) 888
ScrFI CCNGG 2 cut(s) 591, 925
SinI GGWCC 2 cut(s) 25, 869
SmiMI CAYNNNNRTG 1 cut(s) 519
SmlI CTYRAG 2 cut(s) 67, 814
SmoI CTYRAG 2 cut(s) 67, 814
Sse9I AATT 4 cut(s) 29, 434, 460, 779
SsiI CCGC 3 cut(s) 17, 269, 720
SspI AATATT 1 cut(s) 60
SspMI CTAG 3 cut(s) 252, 995, 1161
StyD4I CCNGG 2 cut(s) 589, 923
StyI CCWWGG 3 cut(s) 400, 770, 964
TaaI ACNGT 4 cut(s) 419, 497, 946, 1058
TaiI ACGT 3 cut(s) 14, 259, 328
TaqI TCGA 1 cut(s) 200
TaqII GACCGA 2 cut(s) 339, 1091
TasI AATT 4 cut(s) 29, 434, 460, 779
TatI WGTACW 1 cut(s) 843
TfiI GAWTC 3 cut(s) 586, 898, 1129
Tru1I TTAA 2 cut(s) 32, 1146
Tru9I TTAA 2 cut(s) 32, 1146
TscAI CASTG 5 cut(s) 424, 559, 871, 951, 991
TseFI GTSAC 3 cut(s) 491, 614, 982
TseI GCWGC 3 cut(s) 445, 647, 1157
Tsp45I GTSAC 3 cut(s) 491, 614, 982
TspDTI ATGAA 4 cut(s) 156, 426, 642, 1142
TspGWI ACGGA 7 cut(s) 128, 229, 336, 588, 698, 843, 1164
TspRI CASTG 5 cut(s) 424, 559, 871, 951, 991
Tth111I GACNNNGTC 1 cut(s) 980
VpaK11BI GGWCC 2 cut(s) 25, 869
XmiI GTMKAC 2 cut(s) 471, 861
XmnI GAANNNNTTC 1 cut(s) 427
XspI CTAG 3 cut(s) 252, 995, 1161
ZraI GACGTC 2 cut(s) 257, 326
ZrmI AGTACT 1 cut(s) 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.