MD08G1112500.v1.1

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013002
Physical Location & Seq
Reverse (-)
9940108 .. 9943040
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1112500.v1.1.491

Sequence Viewer

Length: 1155 bp
ATGTCGACGGCGGAGATGGAGCAGCTGACCTCCGGAGCCAGCAACCGCATAATCCCGATCCTCAAAACCCTAAGAGCCTTCCTCCTCTTCATCCACACCTTCTTCCTCTCCTTCCTCCTCTTCCTCCTACCCCGCCGTCGCCGCAGCGGAATCTCGGAGTCACTGCTCGATGCGCCGTCGTCGAAGAAGCGAAAGTCGGCTTTCAGGAGAGAGGAGGAGGACACCTTCAGGCGCAGAGCATTGGCTGAGGGGTATCACACCGGCGACGCCCACTGCTGGTACCGCTCCGCCACGTTCTTGTTCTTTGGTGGTCGCAACAACGCCCTCTTCTGCCGCTCATGGCTCCCGGTCTCTGATACTCCAAGGGGTATTTTGATCATCATACATGGGCTTAATGAGCACAGTGGAAGATATGCTGATTTTGCAAGGCAACTAACCTCATGCAACTTTGGGGTGTATGCAATGGACTGGATAGGTCATGGCGGCAGCGATGGATTGCATGGATACGTTCCTTCACTTGACTATGTTGTTGCTGATACTGGTGCTTTCTTGGAGAAGATTAGGTTAGAGAACCCTGGAGTACCATGCTTCCTCTTTGGTCACTCAACAGGAGGAGCTGTTGTTTTGAAGGCGGCTGCCACCCCTCAAATTGAAGGAATGCTAGAGGGTATTGTACTGACATCACCAGCTTTGCGCGTTAAGCCAGCACATCCAATTGTTGGGGCTGTGGCTCCAATTGTTTCTCTGGTCGCTCCCAAGTTCCAGTTTAAAGGTGCAAACAAGAGGGGAATTCCAGTGTCTCGGGATCCTGCACAATTGTTGGCCAAGTACTCTGATCCCCTGGTCTATACAGGTCCAATCAGGGTCCGAACAGGCTATGAGATATTGCGCATCTCCACTCAATTGACTCGGAACTTTAAGTTTGTGAATATCCCCTTCTTTGTTCTTCATGGTACTGCTGATAGAGTCACCGATCCGCTAGCCTCCCAGGACCTCTACAATGAGGCAGCATCCGAGTTCAAAGATATAAAGCTCTATGACGGTTTCTTGCACGACCTTCTCTTTGAGCCGGAGCGTGTAGAGATTGCACAGGACATAATTGACTGGATGGATAAGAAATTGAGTGCTGGCGTCGAAGATGTTAACAGCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

385

Amino Acids

42.77

Weight (kDa)

8.78

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 120 - 358 4.9e-73 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 124 - 345 1.7e-14 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 125 - 333 9.5e-14 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013002)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11650 AT5G11650
fragaria_vesca FvH4_2g35400
malus_domestica MD08G1112500.v1.1 MD15G1091700.v1.1
prunus_persica Prupe.1G446700_v2.0.a1
pyrus_communis pycom08g09340 pycom15g08550
rosa_chinensis RchiOBHm_Chr6g0306501
rosa_laevigata RLG00000010791
rosa_multiflora Rmu_sc0002971.1_g000010
rosa_roxburghii Rroxscaffold_7G00161720
rosa_rugosa Rorug06G0350000
rosa_samantha Rh6AG462100 Rh6BG438700 Rh6CG476400 Rh6DG463100
rosa_wichuraiana Rw6G040280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 890
Acc65I GGTACC 1 cut(s) 279
AccB1I GGYRCC 1 cut(s) 279
AccB7I CCANNNNNTGG 1 cut(s) 719
AccBSI CCGCTC 2 cut(s) 285, 336
AccI GTMKAC 1 cut(s) 5
AccII CGCG 1 cut(s) 696
AccIII TCCGGA 1 cut(s) 32
AclWI GGATC 5 cut(s) 52, 800, 813, 830, 968
AcoI YGGCCR 1 cut(s) 822
AcsI RAATTY 1 cut(s) 789
AcuI CTGAAG 1 cut(s) 211
AcyI GRCGYC 2 cut(s) 267, 1131
AfaI GTAC 5 cut(s) 281, 582, 675, 830, 955
AfiI CCNNNNNNNGG 2 cut(s) 276, 719
AgsI TTSAA 3 cut(s) 628, 653, 1021
AjnI CCWGG 3 cut(s) 574, 840, 987
AluBI AGCT 4 cut(s) 25, 617, 689, 1033
AluI AGCT 4 cut(s) 25, 617, 689, 1033
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 2 cut(s) 355, 804
AlwI GGATC 5 cut(s) 52, 800, 813, 830, 968
Ama87I CYCGRG 1 cut(s) 801
Aor13HI TCCGGA 1 cut(s) 32
AoxI GGCC 1 cut(s) 822
ApeKI GCWGC 5 cut(s) 22, 144, 486, 635, 1007
ApoI RAATTY 1 cut(s) 789
ArsI GACNNNNNNTTYG 2 cut(s) 1046, 1078
Asp718I GGTACC 1 cut(s) 279
AspLEI GCGC 4 cut(s) 175, 234, 696, 891
AspS9I GGNCC 3 cut(s) 854, 865, 991
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 2 cut(s) 675, 961
AsuNHI GCTAGC 1 cut(s) 979
AvaI CYCGRG 1 cut(s) 801
AvaII GGWCC 3 cut(s) 854, 865, 991
BalI TGGCCA 1 cut(s) 824
BamHI GGATCC 1 cut(s) 805
BanI GGYRCC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 402
BbvCI CCTCAGC 1 cut(s) 246
BbvI GCAGC 5 cut(s) 34, 156, 498, 622, 1019
BccI CCATC 3 cut(s) 10, 485, 1102
BceAI ACGGC 3 cut(s) 24, 120, 160
BciT130I CCWGG 3 cut(s) 576, 842, 989
BciVI GTATCC 1 cut(s) 497
BclI TGATCA 1 cut(s) 375
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 2 cut(s) 355, 804
BfaI CTAG 2 cut(s) 662, 980
BfuI GTATCC 1 cut(s) 497
BisI GCNGC 9 cut(s) 23, 142, 145, 334, 484, 487, 633, 636, 1008
BlsI GCNGC 9 cut(s) 24, 143, 146, 335, 485, 488, 634, 637, 1009
BmcAI AGTACT 1 cut(s) 830
Bme1390I CCNGG 4 cut(s) 347, 576, 842, 989
Bme18I GGWCC 3 cut(s) 854, 865, 991
BmeT110I CYCGRG 1 cut(s) 801
BmgT120I GGNCC 3 cut(s) 854, 865, 991
BmiI GGNNCC 6 cut(s) 37, 281, 344, 732, 807, 866
BmrFI CCNGG 4 cut(s) 347, 576, 842, 989
BmsI GCATC 3 cut(s) 160, 900, 1019
BmtI GCTAGC 1 cut(s) 983
BplI GAGNNNNNCTC 2 cut(s) 66, 98
BpmI CTGGAG 1 cut(s) 597
Bpu10I CCTNAGC 1 cut(s) 246
BpuMI CCSGG 1 cut(s) 347
BsaHI GRCGYC 2 cut(s) 267, 1131
BsaI GGTCTC 1 cut(s) 355
BsaJI CCNNGG 4 cut(s) 362, 574, 840, 987
BsaWI WCCGGW 1 cut(s) 32
BsaXI ACNNNNNCTCC 4 cut(s) 603, 606, 633, 636
Bsc4I CCNNNNNNNGG 2 cut(s) 276, 719
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 5 cut(s) 473, 544, 763, 794, 1109
Bse3DI GCAATG 1 cut(s) 468
BseAI TCCGGA 1 cut(s) 32
BseBI CCWGG 3 cut(s) 576, 842, 989
BseDI CCNNGG 4 cut(s) 362, 574, 840, 987
BseGI GGATG 4 cut(s) 90, 709, 1010, 1113
BseLI CCNNNNNNNGG 2 cut(s) 276, 719
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 5 cut(s) 473, 544, 763, 794, 1109
BseRI GAGGAG 5 cut(s) 74, 107, 227, 230, 627
BseXI GCAGC 5 cut(s) 34, 156, 498, 622, 1019
BsgI GTGCAG 1 cut(s) 795
Bsh1236I CGCG 1 cut(s) 696
BshFI GGCC 1 cut(s) 824
BshNI GGYRCC 1 cut(s) 279
BsiHKAI GWGCWC 1 cut(s) 402
BsiHKCI CYCGRG 1 cut(s) 801
BsiSI CCGG 4 cut(s) 33, 261, 347, 1070
BslI CCNNNNNNNGG 2 cut(s) 276, 719
BsmAI GTCTC 2 cut(s) 355, 804
BsmI GAATGC 1 cut(s) 663
BsnI GGCC 1 cut(s) 824
Bso31I GGTCTC 1 cut(s) 355
BsoBI CYCGRG 1 cut(s) 801
Bsp1286I GDGCHC 1 cut(s) 402
Bsp13I TCCGGA 1 cut(s) 32
Bsp143I GATC 5 cut(s) 57, 375, 805, 835, 973
BspANI GGCC 1 cut(s) 824
BspCNI CTCAG 1 cut(s) 238
BspEI TCCGGA 1 cut(s) 32
BspFNI CGCG 1 cut(s) 696
BspLI GGNNCC 6 cut(s) 37, 281, 344, 732, 807, 866
BspOI GCTAGC 1 cut(s) 983
BspPI GGATC 5 cut(s) 52, 800, 813, 830, 968
BspT107I GGYRCC 1 cut(s) 279
BspTNI GGTCTC 1 cut(s) 355
BsrBI CCGCTC 2 cut(s) 285, 336
BsrDI GCAATG 1 cut(s) 468
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 5 cut(s) 473, 544, 763, 794, 1109
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 4 cut(s) 362, 574, 840, 987
BssMI GATC 5 cut(s) 57, 375, 805, 835, 973
BssNI GRCGYC 2 cut(s) 267, 1131
BssT1I CCWWGG 1 cut(s) 362
Bst2UI CCWGG 3 cut(s) 576, 842, 989
Bst4CI ACNGT 2 cut(s) 404, 1043
Bst6I CTCTTC 3 cut(s) 92, 125, 332
BstACI GRCGYC 2 cut(s) 267, 1131
BstC8I GCNNGC 4 cut(s) 40, 705, 981, 1129
BstDEI CTNAG 2 cut(s) 71, 246
BstF5I GGATG 4 cut(s) 90, 709, 1010, 1113
BstFNI CGCG 1 cut(s) 696
BstHHI GCGC 4 cut(s) 175, 234, 696, 891
BstKTI GATC 5 cut(s) 60, 378, 808, 838, 976
BstMAI GTCTC 2 cut(s) 355, 804
BstMBI GATC 5 cut(s) 57, 375, 805, 835, 973
BstMWI GCNNNNNNNGC 6 cut(s) 141, 172, 282, 397, 422, 700
BstNI CCWGG 3 cut(s) 576, 842, 989
BstSCI CCNGG 4 cut(s) 345, 574, 840, 987
BstUI CGCG 1 cut(s) 696
BstV1I GCAGC 5 cut(s) 34, 156, 498, 622, 1019
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
BsuI GTATCC 1 cut(s) 497
BsuRI GGCC 1 cut(s) 824
BtgZI GCGATG 1 cut(s) 504
BtsCI GGATG 4 cut(s) 90, 709, 1010, 1113
BtsI GCAGTG 2 cut(s) 161, 271
BtsIMutI CAGTG 4 cut(s) 161, 271, 409, 801
Cac8I GCNNGC 4 cut(s) 40, 705, 981, 1129
CfoI GCGC 4 cut(s) 175, 234, 696, 891
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 3 cut(s) 854, 865, 991
CseI GACGC 2 cut(s) 275, 1120
Csp6I GTAC 5 cut(s) 280, 581, 674, 829, 954
CviAII CATG 7 cut(s) 339, 386, 441, 479, 500, 585, 950
CviQI GTAC 5 cut(s) 280, 581, 674, 829, 954
DdeI CTNAG 2 cut(s) 71, 246
DpnI GATC 5 cut(s) 59, 377, 807, 837, 975
DpnII GATC 5 cut(s) 57, 375, 805, 835, 973
DraI TTTAAA 1 cut(s) 769
EaeI YGGCCR 1 cut(s) 822
Eam1104I CTCTTC 3 cut(s) 92, 125, 332
EarI CTCTTC 3 cut(s) 92, 125, 332
EciI GGCGGA 2 cut(s) 26, 277
Eco130I CCWWGG 1 cut(s) 362
Eco31I GGTCTC 1 cut(s) 355
Eco47I GGWCC 3 cut(s) 854, 865, 991
Eco57I CTGAAG 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 801
EcoO109I RGGNCCY 1 cut(s) 991
EcoRI GAATTC 1 cut(s) 789
EcoRII CCWGG 3 cut(s) 574, 840, 987
EcoT14I CCWWGG 1 cut(s) 362
ErhI CCWWGG 1 cut(s) 362
FaeI CATG 7 cut(s) 342, 389, 444, 482, 503, 588, 953
FatI CATG 7 cut(s) 338, 385, 440, 478, 499, 584, 949
FauI CCCGC 1 cut(s) 140
FbaI TGATCA 1 cut(s) 375
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 9 cut(s) 23, 142, 145, 334, 484, 487, 633, 636, 1008
FokI GGATG 4 cut(s) 77, 696, 997, 1120
Fsp4HI GCNGC 9 cut(s) 23, 142, 145, 334, 484, 487, 633, 636, 1008
FspBI CTAG 2 cut(s) 662, 980
FspI TGCGCA 1 cut(s) 890
GlaI GCGC 4 cut(s) 174, 233, 695, 890
GluI GCNGC 9 cut(s) 23, 142, 145, 334, 484, 487, 633, 636, 1008
GsuI CTGGAG 1 cut(s) 597
HaeIII GGCC 1 cut(s) 824
HapII CCGG 4 cut(s) 33, 261, 347, 1070
HgaI GACGC 2 cut(s) 275, 1120
HhaI GCGC 4 cut(s) 175, 234, 696, 891
Hin1I GRCGYC 2 cut(s) 267, 1131
Hin1II CATG 7 cut(s) 342, 389, 444, 482, 503, 588, 953
Hin6I GCGC 4 cut(s) 173, 232, 694, 889
HinP1I GCGC 4 cut(s) 173, 232, 694, 889
HincII GTYRAC 2 cut(s) 6, 1144
HindII GTYRAC 2 cut(s) 6, 1144
HinfI GANTC 4 cut(s) 150, 158, 907, 966
HpaI GTTAAC 1 cut(s) 1144
HpaII CCGG 4 cut(s) 33, 261, 347, 1070
HphI GGTGA 2 cut(s) 675, 961
Hpy166II GTNNAC 2 cut(s) 6, 1144
Hpy188I TCNGA 6 cut(s) 157, 355, 835, 869, 912, 1015
Hpy188III TCNNGA 4 cut(s) 33, 55, 205, 803
Hpy8I GTNNAC 2 cut(s) 6, 1144
Hpy99I CGWCG 6 cut(s) 10, 141, 181, 184, 269, 1136
HpyAV CCTTC 9 cut(s) 88, 109, 121, 235, 522, 622, 647, 946, 1067
HpyCH4III ACNGT 2 cut(s) 404, 1043
HpyCH4IV ACGT 2 cut(s) 293, 507
HpyCH4V TGCA 8 cut(s) 425, 444, 461, 499, 776, 812, 1051, 1088
HpyF10VI GCNNNNNNNGC 6 cut(s) 141, 172, 282, 397, 422, 700
HpyF3I CTNAG 2 cut(s) 71, 246
HpySE526I ACGT 2 cut(s) 293, 507
Hsp92I GRCGYC 2 cut(s) 267, 1131
Hsp92II CATG 7 cut(s) 342, 389, 444, 482, 503, 588, 953
HspAI GCGC 4 cut(s) 173, 232, 694, 889
Kpn2I TCCGGA 1 cut(s) 32
KpnI GGTACC 1 cut(s) 283
Ksp22I TGATCA 1 cut(s) 375
KspAI GTTAAC 1 cut(s) 1144
Kzo9I GATC 5 cut(s) 57, 375, 805, 835, 973
LmnI GCTCC 8 cut(s) 19, 35, 290, 348, 614, 736, 757, 1072
Lsp1109I GCAGC 5 cut(s) 34, 156, 498, 622, 1019
LweI GCATC 3 cut(s) 160, 900, 1019
MaeI CTAG 2 cut(s) 662, 980
MaeII ACGT 2 cut(s) 293, 507
MaeIII GTNAC 3 cut(s) 159, 599, 967
MalI GATC 5 cut(s) 59, 377, 807, 837, 975
MbiI CCGCTC 2 cut(s) 285, 336
MboI GATC 5 cut(s) 57, 375, 805, 835, 973
MboII GAAGA 9 cut(s) 79, 94, 112, 196, 319, 420, 568, 938, 1148
MfeI CAATTG 4 cut(s) 714, 735, 815, 902
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 1 cut(s) 402
MlsI TGGCCA 1 cut(s) 824
MluCI AATT 8 cut(s) 648, 714, 735, 789, 815, 902, 1098, 1118
MluNI TGGCCA 1 cut(s) 824
MlyI GAGTC 3 cut(s) 167, 901, 975
Mox20I TGGCCA 1 cut(s) 824
MroI TCCGGA 1 cut(s) 32
MscI TGGCCA 1 cut(s) 824
MseI TTAA 5 cut(s) 393, 699, 768, 918, 1143
Msp20I TGGCCA 1 cut(s) 824
MspA1I CMGCKG 2 cut(s) 25, 147
MspI CCGG 4 cut(s) 33, 261, 347, 1070
MspR9I CCNGG 4 cut(s) 347, 576, 842, 989
MunI CAATTG 4 cut(s) 714, 735, 815, 902
Mva1269I GAATGC 1 cut(s) 663
MvaI CCWGG 3 cut(s) 576, 842, 989
MvnI CGCG 1 cut(s) 696
MwoI GCNNNNNNNGC 6 cut(s) 141, 172, 282, 397, 422, 700
NciI CCSGG 1 cut(s) 347
NdeII GATC 5 cut(s) 57, 375, 805, 835, 973
NheI GCTAGC 1 cut(s) 979
NlaIII CATG 7 cut(s) 342, 389, 444, 482, 503, 588, 953
NlaIV GGNNCC 6 cut(s) 37, 281, 344, 732, 807, 866
NmuCI GTSAC 3 cut(s) 159, 599, 967
NsbI TGCGCA 1 cut(s) 890
PctI GAATGC 1 cut(s) 663
PfeI GAWTC 1 cut(s) 150
PflMI CCANNNNNTGG 1 cut(s) 719
PkrI GCNGC 9 cut(s) 24, 143, 146, 335, 485, 488, 634, 637, 1009
PleI GAGTC 3 cut(s) 166, 901, 974
PpsI GAGTC 3 cut(s) 166, 901, 974
PpuMI RGGWCCY 1 cut(s) 991
Psp5II RGGWCCY 1 cut(s) 991
Psp6I CCWGG 3 cut(s) 574, 840, 987
PspGI CCWGG 3 cut(s) 574, 840, 987
PspN4I GGNNCC 6 cut(s) 37, 281, 344, 732, 807, 866
PspPI GGNCC 3 cut(s) 854, 865, 991
PspPPI RGGWCCY 1 cut(s) 991
PsuI RGATCY 1 cut(s) 805
PvuII CAGCTG 1 cut(s) 25
RsaI GTAC 5 cut(s) 281, 582, 675, 830, 955
RsaNI GTAC 5 cut(s) 280, 581, 674, 829, 954
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 5 cut(s) 393, 699, 768, 918, 1143
SatI GCNGC 9 cut(s) 23, 142, 145, 334, 484, 487, 633, 636, 1008
Sau3AI GATC 5 cut(s) 57, 375, 805, 835, 973
Sau96I GGNCC 3 cut(s) 854, 865, 991
ScaI AGTACT 1 cut(s) 830
SchI GAGTC 3 cut(s) 167, 901, 975
ScrFI CCNGG 4 cut(s) 347, 576, 842, 989
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 3 cut(s) 160, 900, 1019
SgrAI CRCCGGYG 1 cut(s) 260
SinI GGWCC 3 cut(s) 854, 865, 991
Sse9I AATT 8 cut(s) 648, 714, 735, 789, 815, 902, 1098, 1118
SspMI CTAG 2 cut(s) 662, 980
StyD4I CCNGG 4 cut(s) 345, 574, 840, 987
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 2 cut(s) 404, 1043
TaiI ACGT 2 cut(s) 296, 510
TaqI TCGA 4 cut(s) 5, 168, 182, 1134
TasI AATT 8 cut(s) 648, 714, 735, 789, 815, 902, 1098, 1118
TatI WGTACW 2 cut(s) 673, 828
TauI GCSGC 4 cut(s) 144, 336, 486, 635
TfiI GAWTC 1 cut(s) 150
Tru1I TTAA 5 cut(s) 393, 699, 768, 918, 1143
Tru9I TTAA 5 cut(s) 393, 699, 768, 918, 1143
TscAI CASTG 4 cut(s) 168, 278, 409, 801
TseFI GTSAC 3 cut(s) 159, 599, 967
TseI GCWGC 5 cut(s) 22, 144, 486, 635, 1007
Tsp45I GTSAC 3 cut(s) 159, 599, 967
TspDTI ATGAA 2 cut(s) 79, 938
TspRI CASTG 4 cut(s) 168, 278, 409, 801
Van91I CCANNNNNTGG 1 cut(s) 719
VpaK11BI GGWCC 3 cut(s) 854, 865, 991
XapI RAATTY 1 cut(s) 789
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 2 cut(s) 662, 980
ZrmI AGTACT 1 cut(s) 830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.