RchiOBHm_Chr6g0306501

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013002
Physical Location & Seq
Reverse (-)
65176637 .. 65179311
2675 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27552

Sequence Viewer

Length: 1152 bp
ATGTCGACGGCGGAGATGGAGCAACTGACCTCAGGAGCGAGCAACCGCATCATCCCGCTCCTCAAGGCCCTGAGGACCTCCCTCCTCTTCGTCCACACCGTCTTCCTCTCGCTGCTCCTCTTCGTCTTCCGACGCCGCCGCCACTGCCTGTCGGAATCGCTCACGGAGTCGACGGCCAAGAAGCGGAAATCGGCGTGGAGGAGAGAGGAGGAGGACACTTTCAGACGGAGAGCTTTGGCCGAGGAACTCCACACCGGAGACGGAGACTGCCGGTTCCGTCGCGACACCTTCTTGTTCTACGGTGTAAGGCAAAGCGCCTTATTCTGCCGCTCATGGTTTCCGGTCTCCGGTAATCCAAGGGCTATTTTGGTCATCATACATGGGCTGAATGAACACAGTGGAAGATATGCTGATTTTGCGAGGCAACTAACCTCATGCAACTTTGGGGTTTATGCAATGGACTGGATAGGTCATGGTGGCAGTGATGGATTGCATGGCTATGTTCCTTCACTTGACCATGTTGTTGCTGATACTGGGGATTTCTTGGAAAAGATTAGATTAGAGAACCCTGGAGCACCGTGCTTTCTCTTTGGTCACTCAACTGGAGGAGCTGTGGTTTTGAAGGCGGCAACCTATGCTAAAATTGAAGAAAAGGTGGAAGGTATAATACTGACCTCACCAGCTTTGCGTGTTAAGCCTGCACATCCAATTGTTGGGGCTGTGGCTCCACTTGTTTCTTTGGTCGCTCCCAAGTTCCAGTTTAAAGGTGCAAACAAAAGGGGAATTCCAGTATCTAGAGATCCTGCAGCACTGGTAGCCAAGTATTCCGATCCCCTGGTCTATACAGGGCCAATAAGGGTCAGAACAGGCTATGAGATATTGCGTATCTCTGCTCACTTGACGAGAAACTTTAAGTTTGTGACTGTACCCTTCTTTGTTCTTCATGGGACTGCTGATAGAGTCACGGATCCTCTGGCCTCTCAGGATCTGTACACAGAGGCAGCGTCTGAGTTCAAAGACATAAAGCTCTATGATGGTTTCTTGCACGATCTCCTGTTTGAGCCTGAACGCGAAGAGATTGCGCAGGATATAATTGACTGGATGGAGAAGCGATTAGGTGCTGGCCTTGAAGACGTTACCAACTTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

383

Amino Acids

42.84

Weight (kDa)

8.51

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 118 - 356 5e-75 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 122 - 337 6.2e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 123 - 330 6.3e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013002)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11650 AT5G11650
fragaria_vesca FvH4_2g35400
malus_domestica MD08G1112500.v1.1 MD15G1091700.v1.1
prunus_persica Prupe.1G446700_v2.0.a1
pyrus_communis pycom08g09340 pycom15g08550
rosa_chinensis RchiOBHm_Chr6g0306501
rosa_laevigata RLG00000010791
rosa_multiflora Rmu_sc0002971.1_g000010
rosa_roxburghii Rroxscaffold_7G00161720
rosa_rugosa Rorug06G0350000
rosa_samantha Rh6AG462100 Rh6BG438700 Rh6CG476400 Rh6DG463100
rosa_wichuraiana Rw6G040280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1083
AccB7I CCANNNNNTGG 1 cut(s) 713
AccBSI CCGCTC 2 cut(s) 58, 330
AccI GTMKAC 2 cut(s) 5, 170
AccII CGCG 2 cut(s) 282, 1071
AciI CCGC 8 cut(s) 11, 46, 56, 136, 139, 184, 328, 626
AclWI GGATC 5 cut(s) 794, 824, 962, 975, 993
AcoI YGGCCR 2 cut(s) 174, 237
AcsI RAATTY 1 cut(s) 783
AcyI GRCGYC 1 cut(s) 133
AfaI GTAC 2 cut(s) 927, 992
AfiI CCNNNNNNNGG 3 cut(s) 183, 347, 713
AgsI TTSAA 4 cut(s) 622, 647, 1015, 1130
AjnI CCWGG 2 cut(s) 568, 834
AluBI AGCT 4 cut(s) 233, 611, 683, 1027
AluI AGCT 4 cut(s) 233, 611, 683, 1027
Alw21I GWGCWC 1 cut(s) 577
Alw26I GTCTC 3 cut(s) 252, 258, 349
AlwI GGATC 5 cut(s) 794, 824, 962, 975, 993
AlwNI CAGNNNCTG 2 cut(s) 988, 1007
AoxI GGCC 6 cut(s) 66, 174, 237, 848, 975, 1123
ApeKI GCWGC 3 cut(s) 112, 806, 1001
ApoI RAATTY 1 cut(s) 783
AspLEI GCGC 2 cut(s) 317, 1084
AspS9I GGNCC 3 cut(s) 67, 75, 848
AsuHPI GGTGA 1 cut(s) 669
AvaII GGWCC 1 cut(s) 75
AxyI CCTNAGG 2 cut(s) 31, 71
BamHI GGATCC 1 cut(s) 967
BarI GAAGNNNNNNTAC 2 cut(s) 651, 683
BbsI GAAGAC 3 cut(s) 94, 118, 1137
Bbv12I GWGCWC 1 cut(s) 577
BbvI GCAGC 3 cut(s) 99, 818, 1013
BccI CCATC 4 cut(s) 10, 479, 1028, 1096
BceAI ACGGC 2 cut(s) 24, 189
BcgI CGANNNNNNTGC 2 cut(s) 1061, 1095
BciT130I CCWGG 2 cut(s) 570, 836
BcoDI GTCTC 3 cut(s) 252, 258, 349
BfaI CTAG 1 cut(s) 795
BfmI CTRYAG 1 cut(s) 804
BfoI RGCGCY 1 cut(s) 318
BisI GCNGC 7 cut(s) 113, 136, 139, 328, 627, 807, 1002
BlsI GCNGC 7 cut(s) 114, 137, 140, 329, 628, 808, 1003
Bme1390I CCNGG 2 cut(s) 570, 836
Bme18I GGWCC 1 cut(s) 75
BmgT120I GGNCC 3 cut(s) 67, 75, 848
BmiI GGNNCC 3 cut(s) 275, 726, 969
BmrFI CCNGG 2 cut(s) 570, 836
BmrI ACTGGG 1 cut(s) 543
BmsI GCATC 1 cut(s) 57
BmuI ACTGGG 1 cut(s) 543
BpiI GAAGAC 3 cut(s) 94, 118, 1137
BpmI CTGGAG 2 cut(s) 591, 624
BpuEI CTTGAG 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 133
BsaI GGTCTC 1 cut(s) 349
BsaJI CCNNGG 4 cut(s) 240, 356, 568, 834
BsaWI WCCGGW 3 cut(s) 254, 340, 347
BsaXI ACNNNNNCTCC 4 cut(s) 597, 600, 627, 630
Bsc4I CCNNNNNNNGG 3 cut(s) 183, 347, 713
Bse118I RCCGGY 1 cut(s) 270
Bse1I ACTGG 7 cut(s) 467, 538, 607, 757, 788, 816, 1103
Bse21I CCTNAGG 2 cut(s) 31, 71
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 2 cut(s) 570, 836
BseDI CCNNGG 4 cut(s) 240, 356, 568, 834
BseGI GGATG 3 cut(s) 51, 703, 1107
BseLI CCNNNNNNNGG 3 cut(s) 183, 347, 713
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 4 cut(s) 45, 62, 995, 999
BseNI ACTGG 7 cut(s) 467, 538, 607, 757, 788, 816, 1103
BseRI GAGGAG 7 cut(s) 50, 74, 107, 214, 221, 224, 621
BseXI GCAGC 3 cut(s) 99, 818, 1013
BsgI GTGCAG 1 cut(s) 684
Bsh1236I CGCG 2 cut(s) 282, 1071
BshFI GGCC 6 cut(s) 68, 176, 239, 850, 977, 1125
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 4 cut(s) 255, 271, 341, 348
BslFI GGGAC 1 cut(s) 961
BslI CCNNNNNNNGG 3 cut(s) 183, 347, 713
BsmAI GTCTC 3 cut(s) 252, 258, 349
BsmBI CGTCTC 1 cut(s) 252
BsmFI GGGAC 1 cut(s) 961
BsnI GGCC 6 cut(s) 68, 176, 239, 850, 977, 1125
Bso31I GGTCTC 1 cut(s) 349
Bsp1286I GDGCHC 1 cut(s) 577
Bsp1407I TGTACA 1 cut(s) 990
Bsp143I GATC 5 cut(s) 799, 829, 967, 985, 1048
Bsp68I TCGCGA 1 cut(s) 282
BspACI CCGC 8 cut(s) 11, 46, 56, 136, 139, 184, 328, 626
BspANI GGCC 6 cut(s) 68, 176, 239, 850, 977, 1125
BspCNI CTCAG 4 cut(s) 44, 63, 994, 1000
BspFNI CGCG 2 cut(s) 282, 1071
BspLI GGNNCC 3 cut(s) 275, 726, 969
BspMAI CTGCAG 1 cut(s) 808
BspPI GGATC 5 cut(s) 794, 824, 962, 975, 993
BspTNI GGTCTC 1 cut(s) 349
BsrBI CCGCTC 2 cut(s) 58, 330
BsrDI GCAATG 1 cut(s) 462
BsrFI RCCGGY 1 cut(s) 270
BsrGI TGTACA 1 cut(s) 990
BsrI ACTGG 7 cut(s) 467, 538, 607, 757, 788, 816, 1103
BssAI RCCGGY 1 cut(s) 270
BssECI CCNNGG 4 cut(s) 240, 356, 568, 834
BssMI GATC 5 cut(s) 799, 829, 967, 985, 1048
BssNI GRCGYC 1 cut(s) 133
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 2 cut(s) 570, 836
Bst4CI ACNGT 5 cut(s) 100, 302, 398, 579, 925
Bst6I CTCTTC 3 cut(s) 92, 125, 1068
BstACI GRCGYC 1 cut(s) 133
BstAPI GCANNNNNTGC 1 cut(s) 635
BstAUI TGTACA 1 cut(s) 990
BstC8I GCNNGC 3 cut(s) 40, 699, 1123
BstDEI CTNAG 4 cut(s) 31, 71, 981, 1008
BstF5I GGATG 3 cut(s) 51, 703, 1107
BstFNI CGCG 2 cut(s) 282, 1071
BstH2I RGCGCY 1 cut(s) 318
BstHHI GCGC 2 cut(s) 317, 1084
BstKTI GATC 5 cut(s) 802, 832, 970, 988, 1051
BstMAI GTCTC 3 cut(s) 252, 258, 349
BstMBI GATC 5 cut(s) 799, 829, 967, 985, 1048
BstMWI GCNNNNNNNGC 5 cut(s) 144, 416, 635, 694, 815
BstNI CCWGG 2 cut(s) 570, 836
BstSCI CCNGG 2 cut(s) 568, 834
BstSFI CTRYAG 1 cut(s) 804
BstUI CGCG 2 cut(s) 282, 1071
BstV1I GCAGC 3 cut(s) 99, 818, 1013
BstV2I GAAGAC 3 cut(s) 94, 118, 1137
BstX2I RGATCY 3 cut(s) 799, 967, 985
BstYI RGATCY 3 cut(s) 799, 967, 985
Bsu36I CCTNAGG 2 cut(s) 31, 71
BsuRI GGCC 6 cut(s) 68, 176, 239, 850, 977, 1125
BtsCI GGATG 3 cut(s) 51, 703, 1107
BtsI GCAGTG 2 cut(s) 142, 487
BtsIMutI CAGTG 4 cut(s) 142, 403, 487, 809
BtuMI TCGCGA 1 cut(s) 282
Cac8I GCNNGC 3 cut(s) 40, 699, 1123
CaiI CAGNNNCTG 2 cut(s) 988, 1007
CfoI GCGC 2 cut(s) 317, 1084
Cfr10I RCCGGY 1 cut(s) 270
Cfr13I GGNCC 3 cut(s) 67, 75, 848
CseI GACGC 2 cut(s) 141, 993
Csp6I GTAC 2 cut(s) 926, 991
CviAII CATG 7 cut(s) 333, 380, 435, 473, 494, 518, 944
CviQI GTAC 2 cut(s) 926, 991
DdeI CTNAG 4 cut(s) 31, 71, 981, 1008
DpnI GATC 5 cut(s) 801, 831, 969, 987, 1050
DpnII GATC 5 cut(s) 799, 829, 967, 985, 1048
DraI TTTAAA 1 cut(s) 763
EaeI YGGCCR 2 cut(s) 174, 237
Eam1104I CTCTTC 3 cut(s) 92, 125, 1068
EarI CTCTTC 3 cut(s) 92, 125, 1068
EciI GGCGGA 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 349
Eco47I GGWCC 1 cut(s) 75
Eco81I CCTNAGG 2 cut(s) 31, 71
EcoO109I RGGNCCY 2 cut(s) 67, 75
EcoRI GAATTC 1 cut(s) 783
EcoRII CCWGG 2 cut(s) 568, 834
EcoT14I CCWWGG 1 cut(s) 356
ErhI CCWWGG 1 cut(s) 356
Esp3I CGTCTC 1 cut(s) 252
FaeI CATG 7 cut(s) 336, 383, 438, 476, 497, 521, 947
FaqI GGGAC 1 cut(s) 961
FatI CATG 7 cut(s) 332, 379, 434, 472, 493, 517, 943
FauI CCCGC 1 cut(s) 63
FblI GTMKAC 2 cut(s) 5, 170
Fnu4HI GCNGC 7 cut(s) 113, 136, 139, 328, 627, 807, 1002
FokI GGATG 3 cut(s) 38, 690, 1114
Fsp4HI GCNGC 7 cut(s) 113, 136, 139, 328, 627, 807, 1002
FspBI CTAG 1 cut(s) 795
FspI TGCGCA 1 cut(s) 1083
GlaI GCGC 2 cut(s) 316, 1083
GluI GCNGC 7 cut(s) 113, 136, 139, 328, 627, 807, 1002
GsuI CTGGAG 2 cut(s) 591, 624
HaeII RGCGCY 1 cut(s) 318
HaeIII GGCC 6 cut(s) 68, 176, 239, 850, 977, 1125
HapII CCGG 4 cut(s) 255, 271, 341, 348
HgaI GACGC 2 cut(s) 141, 993
HhaI GCGC 2 cut(s) 317, 1084
Hin1I GRCGYC 1 cut(s) 133
Hin1II CATG 7 cut(s) 336, 383, 438, 476, 497, 521, 947
Hin6I GCGC 2 cut(s) 315, 1082
HinP1I GCGC 2 cut(s) 315, 1082
HincII GTYRAC 2 cut(s) 6, 171
HindII GTYRAC 2 cut(s) 6, 171
HinfI GANTC 3 cut(s) 155, 167, 960
HpaII CCGG 4 cut(s) 255, 271, 341, 348
HphI GGTGA 1 cut(s) 669
Hpy166II GTNNAC 4 cut(s) 6, 94, 171, 993
Hpy188I TCNGA 6 cut(s) 131, 154, 224, 829, 863, 1009
Hpy188III TCNNGA 4 cut(s) 33, 281, 795, 983
Hpy8I GTNNAC 4 cut(s) 6, 94, 171, 993
Hpy99I CGWCG 4 cut(s) 10, 135, 175, 282
HpyAV CCTTC 5 cut(s) 298, 516, 616, 653, 940
HpyCH4III ACNGT 5 cut(s) 100, 302, 398, 579, 925
HpyCH4IV ACGT 1 cut(s) 1134
HpyCH4V TGCA 8 cut(s) 438, 455, 493, 701, 770, 806, 1045, 1148
HpyF10VI GCNNNNNNNGC 5 cut(s) 144, 416, 635, 694, 815
HpyF3I CTNAG 4 cut(s) 31, 71, 981, 1008
HpySE526I ACGT 1 cut(s) 1134
Hsp92I GRCGYC 1 cut(s) 133
Hsp92II CATG 7 cut(s) 336, 383, 438, 476, 497, 521, 947
HspAI GCGC 2 cut(s) 315, 1082
Kzo9I GATC 5 cut(s) 799, 829, 967, 985, 1048
LmnI GCTCC 8 cut(s) 19, 35, 63, 120, 572, 608, 730, 751
Lsp1109I GCAGC 3 cut(s) 99, 818, 1013
LweI GCATC 1 cut(s) 57
MaeI CTAG 1 cut(s) 795
MaeII ACGT 1 cut(s) 1134
MaeIII GTNAC 4 cut(s) 593, 919, 961, 1135
MalI GATC 5 cut(s) 801, 831, 969, 987, 1050
MbiI CCGCTC 2 cut(s) 58, 330
MboI GATC 5 cut(s) 799, 829, 967, 985, 1048
MboII GAAGA 9 cut(s) 79, 94, 112, 118, 414, 659, 932, 1085, 1142
MfeI CAATTG 1 cut(s) 708
MflI RGATCY 3 cut(s) 799, 967, 985
MhlI GDGCHC 1 cut(s) 577
MluCI AATT 4 cut(s) 642, 708, 783, 1092
MlyI GAGTC 2 cut(s) 176, 969
MmeI TCCRAC 2 cut(s) 132, 154
MseI TTAA 3 cut(s) 693, 762, 912
MslI CAYNNNNRTG 1 cut(s) 498
MspI CCGG 4 cut(s) 255, 271, 341, 348
MspR9I CCNGG 2 cut(s) 570, 836
MunI CAATTG 1 cut(s) 708
MvaI CCWGG 2 cut(s) 570, 836
MvnI CGCG 2 cut(s) 282, 1071
MwoI GCNNNNNNNGC 5 cut(s) 144, 416, 635, 694, 815
NdeII GATC 5 cut(s) 799, 829, 967, 985, 1048
NlaIII CATG 7 cut(s) 336, 383, 438, 476, 497, 521, 947
NlaIV GGNNCC 3 cut(s) 275, 726, 969
NmeAIII GCCGAG 1 cut(s) 265
NmuCI GTSAC 3 cut(s) 593, 919, 961
NruI TCGCGA 1 cut(s) 282
NsbI TGCGCA 1 cut(s) 1083
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PfeI GAWTC 1 cut(s) 155
PflMI CCANNNNNTGG 1 cut(s) 713
PkrI GCNGC 7 cut(s) 114, 137, 140, 329, 628, 808, 1003
PleI GAGTC 2 cut(s) 175, 968
PpsI GAGTC 2 cut(s) 175, 968
PpuMI RGGWCCY 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 75
Psp6I CCWGG 2 cut(s) 568, 834
PspGI CCWGG 2 cut(s) 568, 834
PspN4I GGNNCC 3 cut(s) 275, 726, 969
PspPI GGNCC 3 cut(s) 67, 75, 848
PspPPI RGGWCCY 1 cut(s) 75
PstI CTGCAG 1 cut(s) 808
PstNI CAGNNNCTG 2 cut(s) 988, 1007
PsuI RGATCY 3 cut(s) 799, 967, 985
RruI TCGCGA 1 cut(s) 282
RsaI GTAC 2 cut(s) 927, 992
RsaNI GTAC 2 cut(s) 926, 991
RseI CAYNNNNRTG 1 cut(s) 498
SalI GTCGAC 2 cut(s) 4, 169
SaqAI TTAA 3 cut(s) 693, 762, 912
SatI GCNGC 7 cut(s) 113, 136, 139, 328, 627, 807, 1002
Sau3AI GATC 5 cut(s) 799, 829, 967, 985, 1048
Sau96I GGNCC 3 cut(s) 67, 75, 848
SchI GAGTC 2 cut(s) 176, 969
ScrFI CCNGG 2 cut(s) 570, 836
SduI GDGCHC 1 cut(s) 577
SfaNI GCATC 1 cut(s) 57
SfcI CTRYAG 1 cut(s) 804
SinI GGWCC 1 cut(s) 75
SmiMI CAYNNNNRTG 1 cut(s) 498
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 4 cut(s) 642, 708, 783, 1092
SsiI CCGC 8 cut(s) 11, 46, 56, 136, 139, 184, 328, 626
SspMI CTAG 1 cut(s) 795
StyD4I CCNGG 2 cut(s) 568, 834
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 5 cut(s) 100, 302, 398, 579, 925
TaiI ACGT 1 cut(s) 1137
TaqI TCGA 2 cut(s) 5, 170
TasI AATT 4 cut(s) 642, 708, 783, 1092
TatI WGTACW 1 cut(s) 990
TauI GCSGC 4 cut(s) 138, 141, 330, 629
TfiI GAWTC 1 cut(s) 155
Tru1I TTAA 3 cut(s) 693, 762, 912
Tru9I TTAA 3 cut(s) 693, 762, 912
TscAI CASTG 4 cut(s) 149, 403, 487, 816
TseFI GTSAC 3 cut(s) 593, 919, 961
TseI GCWGC 3 cut(s) 112, 806, 1001
Tsp45I GTSAC 3 cut(s) 593, 919, 961
TspDTI ATGAA 2 cut(s) 405, 932
TspGWI ACGGA 5 cut(s) 179, 241, 266, 276, 980
TspRI CASTG 4 cut(s) 149, 403, 487, 816
Van91I CCANNNNNTGG 1 cut(s) 713
VpaK11BI GGWCC 1 cut(s) 75
XapI RAATTY 1 cut(s) 783
XbaI TCTAGA 1 cut(s) 794
XmiI GTMKAC 2 cut(s) 5, 170
XspI CTAG 1 cut(s) 795
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.