Rorug06G0350000

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013002
Physical Location & Seq
Forward (+)
49944930 .. 49946105
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0350000.1

Sequence Viewer

Length: 708 bp
ATGGCAGATGCGGAATCTTCACGCCCAATCGATACGGTGAATTGCGATGAGAAGAAGCTCAAGTATCTGGAATTTGTTGAAGTCGCAGCGATTTACGCCGTCCTCTGCTTCTCGAGCCTCTACGAGTACGCGAAGGAAAACGCCGGTCCTCTGAAACCGGGGGTTCAGACTGTGGAAGGCACCGTCAAAATGGTGATCGGACCGGTCTACGAGAAGTTCCAGGACCTTCCTTTCCAGCTCCTCAAATTCGTCGATCACAAGGTGGACGAGTCGCTGAGCGAGTTGCACCGTCACGTGCCGTGCCTGGTAAAGCAGGCGTCGAACATGGCTCTGTCGGTGGCGACGGAAGTTAAGCGAGCCGGGTTGGTGGACGCGGCGCGTAACATCACGGCGAGCGCTTACTCGAAATACGAGCCGATGGCGGAGGAGCTGTACTACAAGTACGAGCCGGTGGCGGAGCAGTACGCCGTCTCGGCTTGGCGGTCGCTGAATCGGCTGCCGCTGGTTCCTCAGGTGGCTCAGATTATGGTCCCCACGGCGGCCTATTGGTCTGAGAAGTACAATCAGGCGGTTTGTTTCACGGCGGAGAGAGGGTTCCCCGTGGCGGCTCACCTGCCGTTGATTCCGACGGAGAGGATTGCGAAGGCGTTTGAGGATGTCTCTCCCGCCGTTTCGACGAATGCCGGAGCCGTCGGCCTGGAACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

235

Amino Acids

26.04

Weight (kDa)

5.46

Isoelectric Point (pI)

40.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
REF PF05755 16 - 218 1.7e-85 Rubber elongation factor protein (REF)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013002)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11650 AT5G11650
fragaria_vesca FvH4_2g35400
malus_domestica MD08G1112500.v1.1 MD15G1091700.v1.1
prunus_persica Prupe.1G446700_v2.0.a1
pyrus_communis pycom08g09340 pycom15g08550
rosa_chinensis RchiOBHm_Chr6g0306501
rosa_laevigata RLG00000010791
rosa_multiflora Rmu_sc0002971.1_g000010
rosa_roxburghii Rroxscaffold_7G00161720
rosa_rugosa Rorug06G0350000
rosa_samantha Rh6AG462100 Rh6BG438700 Rh6CG476400 Rh6DG463100
rosa_wichuraiana Rw6G040280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 621
Acc36I ACCTGC 1 cut(s) 621
AccB1I GGYRCC 1 cut(s) 179
AccI GTMKAC 1 cut(s) 207
AccII CGCG 3 cut(s) 131, 374, 379
AcsI RAATTY 2 cut(s) 71, 245
AcvI CACGTG 1 cut(s) 295
AcyI GRCGYC 1 cut(s) 317
AdeI CACNNNGTG 1 cut(s) 262
AfaI GTAC 5 cut(s) 128, 434, 443, 464, 560
AfeI AGCGCT 1 cut(s) 397
AfiI CCNNNNNNNGG 2 cut(s) 538, 604
AgeI ACCGGT 1 cut(s) 202
AgsI TTSAA 1 cut(s) 80
AjnI CCWGG 3 cut(s) 219, 303, 696
AloI GAACNNNNNNTCC 2 cut(s) 578, 610
AluBI AGCT 3 cut(s) 58, 238, 430
AluI AGCT 3 cut(s) 58, 238, 430
Alw26I GTCTC 2 cut(s) 475, 664
Ama87I CYCGRG 1 cut(s) 112
Aor51HI AGCGCT 1 cut(s) 397
AoxI GGCC 2 cut(s) 540, 694
ApeKI GCWGC 2 cut(s) 86, 496
ApoI RAATTY 2 cut(s) 71, 245
AsiGI ACCGGT 1 cut(s) 202
AspLEI GCGC 2 cut(s) 379, 398
AspS9I GGNCC 4 cut(s) 146, 200, 223, 529
AsuC2I CCSGG 2 cut(s) 159, 361
AsuHPI GGTGA 3 cut(s) 49, 205, 602
AvaI CYCGRG 1 cut(s) 112
AvaII GGWCC 4 cut(s) 146, 200, 223, 529
AxyI CCTNAGG 1 cut(s) 510
BanI GGYRCC 1 cut(s) 179
BbrPI CACGTG 1 cut(s) 295
BbvI GCAGC 2 cut(s) 98, 483
BccI CCATC 1 cut(s) 412
BceAI ACGGC 9 cut(s) 83, 283, 405, 452, 552, 597, 601, 653, 674
BciT130I CCWGG 3 cut(s) 221, 305, 698
BcnI CCSGG 2 cut(s) 159, 361
BcoDI GTCTC 2 cut(s) 475, 664
BfoI RGCGCY 1 cut(s) 399
BfuAI ACCTGC 1 cut(s) 621
BglI GCCNNNNNGGC 1 cut(s) 473
BisI GCNGC 6 cut(s) 87, 375, 497, 500, 540, 606
BlpI GCTNAGC 1 cut(s) 275
BlsI GCNGC 6 cut(s) 88, 376, 498, 501, 541, 607
Bme1390I CCNGG 5 cut(s) 159, 221, 305, 361, 698
Bme18I GGWCC 4 cut(s) 146, 200, 223, 529
BmeT110I CYCGRG 1 cut(s) 112
BmgT120I GGNCC 4 cut(s) 146, 200, 223, 529
BmiI GGNNCC 5 cut(s) 181, 507, 531, 596, 688
BmrFI CCNGG 5 cut(s) 159, 221, 305, 361, 698
BplI GAGNNNNNCTC 2 cut(s) 644, 676
Bpu1102I GCTNAGC 1 cut(s) 275
BpuEI CTTGAG 1 cut(s) 44
BpuMI CCSGG 2 cut(s) 159, 361
Bsa29I ATCGAT 1 cut(s) 30
BsaAI YACGTR 1 cut(s) 295
BsaHI GRCGYC 1 cut(s) 317
BsaJI CCNNGG 3 cut(s) 158, 534, 600
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 4 cut(s) 416, 446, 578, 608
Bsc4I CCNNNNNNNGG 2 cut(s) 538, 604
Bse118I RCCGGY 3 cut(s) 143, 202, 448
Bse21I CCTNAGG 1 cut(s) 510
BseBI CCWGG 3 cut(s) 221, 305, 698
BseCI ATCGAT 1 cut(s) 30
BseDI CCNNGG 3 cut(s) 158, 534, 600
BseGI GGATG 1 cut(s) 661
BseLI CCNNNNNNNGG 2 cut(s) 538, 604
BseMII CTCAG 4 cut(s) 266, 524, 533, 543
BseRI GAGGAG 2 cut(s) 230, 440
BseXI GCAGC 2 cut(s) 98, 483
Bsh1236I CGCG 3 cut(s) 131, 374, 379
Bsh1285I CGRYCG 1 cut(s) 485
BshFI GGCC 2 cut(s) 542, 696
BshNI GGYRCC 1 cut(s) 179
BshTI ACCGGT 1 cut(s) 202
BshVI ATCGAT 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 485
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 6 cut(s) 144, 158, 203, 360, 449, 684
BslFI GGGAC 1 cut(s) 515
BslI CCNNNNNNNGG 2 cut(s) 538, 604
BsmAI GTCTC 2 cut(s) 475, 664
BsmBI CGTCTC 1 cut(s) 475
BsmFI GGGAC 1 cut(s) 515
BsmI GAATGC 1 cut(s) 685
BsnI GGCC 2 cut(s) 542, 696
BsoBI CYCGRG 1 cut(s) 112
Bsp143I GATC 2 cut(s) 195, 253
Bsp1720I GCTNAGC 1 cut(s) 275
BspANI GGCC 2 cut(s) 542, 696
BspCNI CTCAG 4 cut(s) 267, 523, 532, 544
BspDI ATCGAT 1 cut(s) 30
BspFNI CGCG 3 cut(s) 131, 374, 379
BspLI GGNNCC 5 cut(s) 181, 507, 531, 596, 688
BspMI ACCTGC 1 cut(s) 621
BspT107I GGYRCC 1 cut(s) 179
BsrFI RCCGGY 3 cut(s) 143, 202, 448
BssAI RCCGGY 3 cut(s) 143, 202, 448
BssECI CCNNGG 3 cut(s) 158, 534, 600
BssMI GATC 2 cut(s) 195, 253
BssNI GRCGYC 1 cut(s) 317
Bst2UI CCWGG 3 cut(s) 221, 305, 698
Bst4CI ACNGT 4 cut(s) 37, 172, 184, 290
BstACI GRCGYC 1 cut(s) 317
BstBAI YACGTR 1 cut(s) 295
BstC8I GCNNGC 3 cut(s) 315, 357, 394
BstDEI CTNAG 4 cut(s) 275, 510, 519, 552
BstDSI CCRYGG 2 cut(s) 534, 600
BstF5I GGATG 1 cut(s) 661
BstFNI CGCG 3 cut(s) 131, 374, 379
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 2 cut(s) 379, 398
BstKTI GATC 2 cut(s) 198, 256
BstMAI GTCTC 2 cut(s) 475, 664
BstMBI GATC 2 cut(s) 195, 253
BstMCI CGRYCG 1 cut(s) 485
BstMWI GCNNNNNNNGC 4 cut(s) 95, 114, 473, 493
BstNI CCWGG 3 cut(s) 221, 305, 698
BstSCI CCNGG 5 cut(s) 157, 219, 303, 359, 696
BstUI CGCG 3 cut(s) 131, 374, 379
BstV1I GCAGC 2 cut(s) 98, 483
Bsu15I ATCGAT 1 cut(s) 30
Bsu36I CCTNAGG 1 cut(s) 510
BsuRI GGCC 2 cut(s) 542, 696
BsuTUI ATCGAT 1 cut(s) 30
BtgI CCRYGG 2 cut(s) 534, 600
BtgZI GCGATG 1 cut(s) 60
BtsCI GGATG 1 cut(s) 661
BtsIMutI CAGTG 1 cut(s) 703
BveI ACCTGC 1 cut(s) 621
Cac8I GCNNGC 3 cut(s) 315, 357, 394
CfoI GCGC 2 cut(s) 379, 398
Cfr10I RCCGGY 3 cut(s) 143, 202, 448
Cfr13I GGNCC 4 cut(s) 146, 200, 223, 529
ClaI ATCGAT 1 cut(s) 30
CpoI CGGWCCG 1 cut(s) 200
CseI GACGC 2 cut(s) 306, 380
Csp6I GTAC 5 cut(s) 127, 433, 442, 463, 559
CspAI ACCGGT 1 cut(s) 202
CspI CGGWCCG 1 cut(s) 200
CviAII CATG 1 cut(s) 325
CviQI GTAC 5 cut(s) 127, 433, 442, 463, 559
DdeI CTNAG 4 cut(s) 275, 510, 519, 552
DpnI GATC 2 cut(s) 197, 255
DpnII GATC 2 cut(s) 195, 253
DraIII CACNNNGTG 1 cut(s) 262
EciI GGCGGA 3 cut(s) 437, 470, 599
Eco47I GGWCC 4 cut(s) 146, 200, 223, 529
Eco47III AGCGCT 1 cut(s) 397
Eco72I CACGTG 1 cut(s) 295
Eco81I CCTNAGG 1 cut(s) 510
Eco88I CYCGRG 1 cut(s) 112
EcoO109I RGGNCCY 1 cut(s) 223
EcoRII CCWGG 3 cut(s) 219, 303, 696
Esp3I CGTCTC 1 cut(s) 475
FaeI CATG 1 cut(s) 328
FaiI YATR 2 cut(s) 326, 527
FaqI GGGAC 1 cut(s) 515
FatI CATG 1 cut(s) 324
FauI CCCGC 1 cut(s) 673
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 6 cut(s) 87, 375, 497, 500, 540, 606
FokI GGATG 1 cut(s) 668
Fsp4HI GCNGC 6 cut(s) 87, 375, 497, 500, 540, 606
GlaI GCGC 2 cut(s) 378, 397
GluI GCNGC 6 cut(s) 87, 375, 497, 500, 540, 606
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 2 cut(s) 542, 696
HapII CCGG 6 cut(s) 144, 158, 203, 360, 449, 684
HgaI GACGC 2 cut(s) 306, 380
HhaI GCGC 2 cut(s) 379, 398
Hin1I GRCGYC 1 cut(s) 317
Hin1II CATG 1 cut(s) 328
Hin6I GCGC 2 cut(s) 377, 396
HinP1I GCGC 2 cut(s) 377, 396
HinfI GANTC 4 cut(s) 14, 269, 490, 622
HpaII CCGG 6 cut(s) 144, 158, 203, 360, 449, 684
HphI GGTGA 3 cut(s) 49, 205, 602
Hpy166II GTNNAC 3 cut(s) 208, 265, 370
Hpy188I TCNGA 6 cut(s) 153, 168, 200, 522, 553, 627
Hpy188III TCNNGA 2 cut(s) 68, 112
Hpy8I GTNNAC 3 cut(s) 208, 265, 370
Hpy99I CGWCG 6 cut(s) 254, 322, 346, 631, 679, 695
HpyAV CCTTC 4 cut(s) 127, 170, 236, 637
HpyCH4III ACNGT 4 cut(s) 37, 172, 184, 290
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 1 cut(s) 286
HpyF10VI GCNNNNNNNGC 4 cut(s) 95, 114, 473, 493
HpyF3I CTNAG 4 cut(s) 275, 510, 519, 552
HpySE526I ACGT 1 cut(s) 294
Hsp92I GRCGYC 1 cut(s) 317
Hsp92II CATG 1 cut(s) 328
HspAI GCGC 2 cut(s) 377, 396
Kzo9I GATC 2 cut(s) 195, 253
LmnI GCTCC 4 cut(s) 243, 427, 457, 686
Lsp1109I GCAGC 2 cut(s) 98, 483
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 2 cut(s) 290, 380
MalI GATC 2 cut(s) 197, 255
MboI GATC 2 cut(s) 195, 253
MboII GAAGA 2 cut(s) 9, 64
MluCI AATT 3 cut(s) 40, 71, 245
MlyI GAGTC 1 cut(s) 278
MmeI TCCRAC 1 cut(s) 650
MnlI CCTC 9 cut(s) 113, 128, 159, 251, 418, 519, 584, 627, 646
MseI TTAA 1 cut(s) 351
MspA1I CMGCKG 1 cut(s) 502
MspI CCGG 6 cut(s) 144, 158, 203, 360, 449, 684
MspR9I CCNGG 5 cut(s) 159, 221, 305, 361, 698
Mva1269I GAATGC 1 cut(s) 685
MvaI CCWGG 3 cut(s) 221, 305, 698
MvnI CGCG 3 cut(s) 131, 374, 379
MwoI GCNNNNNNNGC 4 cut(s) 95, 114, 473, 493
NciI CCSGG 2 cut(s) 159, 361
NdeII GATC 2 cut(s) 195, 253
NlaIII CATG 1 cut(s) 328
NlaIV GGNNCC 5 cut(s) 181, 507, 531, 596, 688
NmeAIII GCCGAG 1 cut(s) 452
NmuCI GTSAC 1 cut(s) 290
PaeR7I CTCGAG 1 cut(s) 112
PaqCI CACCTGC 1 cut(s) 621
PctI GAATGC 1 cut(s) 685
PfeI GAWTC 3 cut(s) 14, 490, 622
PfoI TCCNGGA 1 cut(s) 219
PinAI ACCGGT 1 cut(s) 202
PkrI GCNGC 6 cut(s) 88, 376, 498, 501, 541, 607
PleI GAGTC 1 cut(s) 277
PmaCI CACGTG 1 cut(s) 295
PmlI CACGTG 1 cut(s) 295
PpsI GAGTC 1 cut(s) 277
Ppu21I YACGTR 1 cut(s) 295
PpuMI RGGWCCY 1 cut(s) 223
Psp5II RGGWCCY 1 cut(s) 223
Psp6I CCWGG 3 cut(s) 219, 303, 696
PspCI CACGTG 1 cut(s) 295
PspGI CCWGG 3 cut(s) 219, 303, 696
PspN4I GGNNCC 5 cut(s) 181, 507, 531, 596, 688
PspPI GGNCC 4 cut(s) 146, 200, 223, 529
PspPPI RGGWCCY 1 cut(s) 223
RsaI GTAC 5 cut(s) 128, 434, 443, 464, 560
RsaNI GTAC 5 cut(s) 127, 433, 442, 463, 559
Rsr2I CGGWCCG 1 cut(s) 200
RsrII CGGWCCG 1 cut(s) 200
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 6 cut(s) 87, 375, 497, 500, 540, 606
Sau3AI GATC 2 cut(s) 195, 253
Sau96I GGNCC 4 cut(s) 146, 200, 223, 529
SchI GAGTC 1 cut(s) 278
ScrFI CCNGG 5 cut(s) 159, 221, 305, 361, 698
SetI ASST 8 cut(s) 60, 228, 240, 264, 297, 432, 516, 615
Sfr274I CTCGAG 1 cut(s) 112
SinI GGWCC 4 cut(s) 146, 200, 223, 529
SlaI CTCGAG 1 cut(s) 112
SmlI CTYRAG 2 cut(s) 59, 112
SmoI CTYRAG 2 cut(s) 59, 112
Sse9I AATT 3 cut(s) 40, 71, 245
StyD4I CCNGG 5 cut(s) 157, 219, 303, 359, 696
TaaI ACNGT 4 cut(s) 37, 172, 184, 290
TaiI ACGT 1 cut(s) 297
TaqI TCGA 6 cut(s) 30, 113, 252, 320, 404, 674
TasI AATT 3 cut(s) 40, 71, 245
TatI WGTACW 2 cut(s) 432, 558
TauI GCSGC 4 cut(s) 377, 502, 542, 608
TfiI GAWTC 3 cut(s) 14, 490, 622
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 2 cut(s) 86, 496
Tsp45I GTSAC 1 cut(s) 290
TspGWI ACGGA 2 cut(s) 359, 644
VpaK11BI GGWCC 4 cut(s) 146, 200, 223, 529
XapI RAATTY 2 cut(s) 71, 245
XhoI CTCGAG 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.