FvH4_2g35400

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0013002
Physical Location & Seq
Forward (+)
26111851 .. 26114562
2712 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g35400.t1

Sequence Viewer

Length: 1161 bp
ATGTCGACGGCGGAGATGGAGCAACTGACCTCGGGGGCCAGCAACCGCATCATCCCGCTCCTCAAGGCTCTGCGGGCCTCTCTCTTATTCGTCCACACCGTCTTCCTCTCGCTCCTCCTCTTCGTCCTCCCCCGCCGCCGCCGCTGCCTCTCGGAGTCGCTCTCCGATTCGGCCGCCAAGAAGCGGAAATCGGCGTGGCGGAGAGAGGAGGAGGACACTTTCAGACGGCGAGCTTTGGCCGAGGGCCTTCACACCGGTGGTGACGGCGACGGAGACTGCCGGTTCCGTCGCGACACCTTCTTGTTCTACGGTGTGAGGCAGAGCGCTTTGTTCTGCCGCTCCTGGTTTCCGGTCTCCGGTAATCCAAGGGGAATTGTGATCATCATACATGGGCTGAATGAACACAGCGGAAGATATGCTGATTTCGCTAGGCAACTAACCTCATGCAACTTTGGGGTGTATGCAATGGACTGGATAGGTCATGGTGGCAGTGATGGATTGCATGGATATGTTCCTTCACTTGACCATGTTGTTGCTGATACTGGGGATTTCTTGGAGAAGATTAGATTAGAGAACCCTGGAGTACCATGCTTCCTCTTTGGTCACTCAACTGGGGGAGCTGTGGTTTTGAAGGCGTCCACCTATGCTAAAATTGAAGAAATGGTGGAAGGTATAATACTGACCTCTCCTGCTTTGCGTGTTAAGCCGGCACATCCAATTGTTGGGGCTGTGGCTCCACTTATTTCTCTGGTCGCTCCCAAGTTCCAGTTTAAAGGTGCAAACAAAAGGGGAATTCCAGTATCAAGAGATCCTGCAGCACTGGTGGCCAAGTACTCCGATCCCCTGGTCTATACAGGGCCAATAAGGGTCAGAACAGGCTACGAGATATTGCGTATCTCTGCTCACTTGATGCGAAACTTTAAGTTTGTGACTGTACCCTTCTTTGTTCTTCATGGGACTGCTGATAGAGTGACGGATCCTCTTGCCTCCCATGATCTGTATAAAGAGGCAGCGTCCGAGTTCAAAGACATCAAGCTCTATGATGGATTCTTGCACGACCTCCTCTTTGAGCCTGAACGTGAAGAGATCGCGCAGGATATAATCGATTGGATGGAGAAGCGATTAGGTGCTGTCCCTGAAGATGTTACCATCTTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

387

Amino Acids

42.97

Weight (kDa)

8.51

Isoelectric Point (pI)

41.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 121 - 359 4e-74 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 125 - 337 1.7e-12 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 126 - 339 1.5e-14 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013002)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11650 AT5G11650
fragaria_vesca FvH4_2g35400
malus_domestica MD08G1112500.v1.1 MD15G1091700.v1.1
prunus_persica Prupe.1G446700_v2.0.a1
pyrus_communis pycom08g09340 pycom15g08550
rosa_chinensis RchiOBHm_Chr6g0306501
rosa_laevigata RLG00000010791
rosa_multiflora Rmu_sc0002971.1_g000010
rosa_roxburghii Rroxscaffold_7G00161720
rosa_rugosa Rorug06G0350000
rosa_samantha Rh6AG462100 Rh6BG438700 Rh6CG476400 Rh6DG463100
rosa_wichuraiana Rw6G040280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 722
AccBSI CCGCTC 2 cut(s) 58, 339
AccI GTMKAC 1 cut(s) 5
AccII CGCG 2 cut(s) 291, 1091
AclWI GGATC 4 cut(s) 803, 833, 971, 984
AcoI YGGCCR 3 cut(s) 171, 237, 825
AcsI RAATTY 1 cut(s) 792
AcuI CTGAAG 1 cut(s) 1158
AcyI GRCGYC 1 cut(s) 635
AfaI GTAC 3 cut(s) 585, 833, 936
AfeI AGCGCT 1 cut(s) 325
AfiI CCNNNNNNNGG 3 cut(s) 183, 356, 722
AgeI ACCGGT 1 cut(s) 254
AgsI TTSAA 3 cut(s) 631, 656, 1024
AjnI CCWGG 3 cut(s) 341, 577, 843
AleI CACNNNNGTG 1 cut(s) 255
AluBI AGCT 3 cut(s) 233, 620, 1036
AluI AGCT 3 cut(s) 233, 620, 1036
Alw26I GTCTC 2 cut(s) 267, 358
AlwI GGATC 4 cut(s) 803, 833, 971, 984
Ama87I CYCGRG 1 cut(s) 31
Aor51HI AGCGCT 1 cut(s) 325
AoxI GGCC 7 cut(s) 36, 75, 171, 237, 244, 825, 857
ApeKI GCWGC 3 cut(s) 144, 815, 1010
ApoI RAATTY 1 cut(s) 792
ArsI GACNNNNNNTTYG 2 cut(s) 1049, 1081
AsiGI ACCGGT 1 cut(s) 254
AspLEI GCGC 2 cut(s) 326, 1093
AspS9I GGNCC 4 cut(s) 36, 75, 244, 857
AsuHPI GGTGA 1 cut(s) 272
AvaI CYCGRG 1 cut(s) 31
BalI TGGCCA 1 cut(s) 827
BamHI GGATCC 1 cut(s) 976
BarI GAAGNNNNNNTAC 2 cut(s) 660, 692
BbsI GAAGAC 1 cut(s) 94
BbvI GCAGC 3 cut(s) 131, 827, 1022
BccI CCATC 5 cut(s) 10, 488, 1037, 1105, 1157
BceAI ACGGC 3 cut(s) 24, 242, 280
BciT130I CCWGG 3 cut(s) 343, 579, 845
BclI TGATCA 1 cut(s) 378
BcoDI GTCTC 2 cut(s) 267, 358
BfaI CTAG 1 cut(s) 429
BfmI CTRYAG 1 cut(s) 813
BfoI RGCGCY 1 cut(s) 327
BisI GCNGC 8 cut(s) 136, 139, 142, 145, 174, 337, 816, 1011
BlsI GCNGC 8 cut(s) 137, 140, 143, 146, 175, 338, 817, 1012
BmcAI AGTACT 1 cut(s) 833
Bme1390I CCNGG 3 cut(s) 343, 579, 845
BmeT110I CYCGRG 1 cut(s) 31
BmgT120I GGNCC 4 cut(s) 36, 75, 244, 857
BmiI GGNNCC 4 cut(s) 37, 284, 735, 978
BmrFI CCNGG 3 cut(s) 343, 579, 845
BmrI ACTGGG 2 cut(s) 552, 621
BmsI GCATC 2 cut(s) 57, 900
BmuI ACTGGG 2 cut(s) 552, 621
BpiI GAAGAC 1 cut(s) 94
BplI GAGNNNNNCTC 2 cut(s) 146, 178
BpmI CTGGAG 1 cut(s) 600
BpuEI CTTGAG 1 cut(s) 47
Bsa29I ATCGAT 1 cut(s) 1104
BsaHI GRCGYC 1 cut(s) 635
BsaI GGTCTC 1 cut(s) 358
BsaJI CCNNGG 5 cut(s) 30, 240, 365, 577, 843
BsaWI WCCGGW 3 cut(s) 254, 349, 356
BsaXI ACNNNNNCTCC 2 cut(s) 609, 639
Bsc4I CCNNNNNNNGG 3 cut(s) 183, 356, 722
Bse118I RCCGGY 3 cut(s) 254, 279, 706
Bse1I ACTGG 6 cut(s) 476, 547, 616, 766, 797, 825
Bse3DI GCAATG 1 cut(s) 471
BseBI CCWGG 3 cut(s) 343, 579, 845
BseCI ATCGAT 1 cut(s) 1104
BseDI CCNNGG 5 cut(s) 30, 240, 365, 577, 843
BseGI GGATG 3 cut(s) 51, 712, 1116
BseLI CCNNNNNNNGG 3 cut(s) 183, 356, 722
BseMI GCAATG 1 cut(s) 471
BseNI ACTGG 6 cut(s) 476, 547, 616, 766, 797, 825
BseRI GAGGAG 6 cut(s) 50, 104, 107, 221, 224, 1052
BseX3I CGGCCG 1 cut(s) 171
BseXI GCAGC 3 cut(s) 131, 827, 1022
Bsh1236I CGCG 2 cut(s) 291, 1091
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 7 cut(s) 38, 77, 173, 239, 246, 827, 859
BshTI ACCGGT 1 cut(s) 254
BshVI ATCGAT 1 cut(s) 1104
BsiEI CGRYCG 1 cut(s) 174
BsiHKCI CYCGRG 1 cut(s) 31
BsiSI CCGG 5 cut(s) 255, 280, 350, 357, 707
BslFI GGGAC 2 cut(s) 970, 1118
BslI CCNNNNNNNGG 3 cut(s) 183, 356, 722
BsmAI GTCTC 2 cut(s) 267, 358
BsmFI GGGAC 2 cut(s) 970, 1118
BsnI GGCC 7 cut(s) 38, 77, 173, 239, 246, 827, 859
Bso31I GGTCTC 1 cut(s) 358
BsoBI CYCGRG 1 cut(s) 31
Bsp143I GATC 6 cut(s) 378, 808, 838, 976, 994, 1086
Bsp68I TCGCGA 1 cut(s) 291
BspANI GGCC 7 cut(s) 38, 77, 173, 239, 246, 827, 859
BspDI ATCGAT 1 cut(s) 1104
BspFNI CGCG 2 cut(s) 291, 1091
BspLI GGNNCC 4 cut(s) 37, 284, 735, 978
BspMAI CTGCAG 1 cut(s) 817
BspPI GGATC 4 cut(s) 803, 833, 971, 984
BspTNI GGTCTC 1 cut(s) 358
BsrBI CCGCTC 2 cut(s) 58, 339
BsrDI GCAATG 1 cut(s) 471
BsrFI RCCGGY 3 cut(s) 254, 279, 706
BsrI ACTGG 6 cut(s) 476, 547, 616, 766, 797, 825
BssAI RCCGGY 3 cut(s) 254, 279, 706
BssECI CCNNGG 5 cut(s) 30, 240, 365, 577, 843
BssMI GATC 6 cut(s) 378, 808, 838, 976, 994, 1086
BssNI GRCGYC 1 cut(s) 635
BssT1I CCWWGG 1 cut(s) 365
Bst2UI CCWGG 3 cut(s) 343, 579, 845
Bst4CI ACNGT 3 cut(s) 100, 311, 934
Bst6I CTCTTC 2 cut(s) 125, 1077
BstACI GRCGYC 1 cut(s) 635
BstC8I GCNNGC 4 cut(s) 40, 75, 231, 708
BstF5I GGATG 3 cut(s) 51, 712, 1116
BstFNI CGCG 2 cut(s) 291, 1091
BstH2I RGCGCY 1 cut(s) 327
BstHHI GCGC 2 cut(s) 326, 1093
BstKTI GATC 6 cut(s) 381, 811, 841, 979, 997, 1089
BstMAI GTCTC 2 cut(s) 267, 358
BstMBI GATC 6 cut(s) 378, 808, 838, 976, 994, 1086
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 6 cut(s) 74, 141, 144, 425, 703, 824
BstNI CCWGG 3 cut(s) 343, 579, 845
BstSCI CCNGG 3 cut(s) 341, 577, 843
BstSFI CTRYAG 1 cut(s) 813
BstUI CGCG 2 cut(s) 291, 1091
BstV1I GCAGC 3 cut(s) 131, 827, 1022
BstV2I GAAGAC 1 cut(s) 94
BstX2I RGATCY 2 cut(s) 808, 976
BstYI RGATCY 2 cut(s) 808, 976
BstZI CGGCCG 1 cut(s) 171
Bsu15I ATCGAT 1 cut(s) 1104
BsuRI GGCC 7 cut(s) 38, 77, 173, 239, 246, 827, 859
BsuTUI ATCGAT 1 cut(s) 1104
BtsCI GGATG 3 cut(s) 51, 712, 1116
BtsI GCAGTG 1 cut(s) 496
BtsIMutI CAGTG 2 cut(s) 496, 818
BtuMI TCGCGA 1 cut(s) 291
Cac8I GCNNGC 4 cut(s) 40, 75, 231, 708
CfoI GCGC 2 cut(s) 326, 1093
Cfr10I RCCGGY 3 cut(s) 254, 279, 706
Cfr13I GGNCC 4 cut(s) 36, 75, 244, 857
ClaI ATCGAT 1 cut(s) 1104
CseI GACGC 2 cut(s) 624, 1002
Csp6I GTAC 3 cut(s) 584, 832, 935
CspAI ACCGGT 1 cut(s) 254
CviAII CATG 8 cut(s) 389, 444, 482, 503, 527, 588, 953, 992
CviQI GTAC 3 cut(s) 584, 832, 935
DpnI GATC 6 cut(s) 380, 810, 840, 978, 996, 1088
DpnII GATC 6 cut(s) 378, 808, 838, 976, 994, 1086
DraI TTTAAA 1 cut(s) 772
EaeI YGGCCR 3 cut(s) 171, 237, 825
EagI CGGCCG 1 cut(s) 171
Eam1104I CTCTTC 2 cut(s) 125, 1077
EarI CTCTTC 2 cut(s) 125, 1077
EciI GGCGGA 2 cut(s) 26, 214
EclXI CGGCCG 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 365
Eco31I GGTCTC 1 cut(s) 358
Eco47III AGCGCT 1 cut(s) 325
Eco52I CGGCCG 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 1158
Eco88I CYCGRG 1 cut(s) 31
EcoO109I RGGNCCY 1 cut(s) 244
EcoRI GAATTC 1 cut(s) 792
EcoRII CCWGG 3 cut(s) 341, 577, 843
EcoT14I CCWWGG 1 cut(s) 365
ErhI CCWWGG 1 cut(s) 365
FaeI CATG 8 cut(s) 392, 447, 485, 506, 530, 591, 956, 995
FaqI GGGAC 2 cut(s) 970, 1118
FatI CATG 8 cut(s) 388, 443, 481, 502, 526, 587, 952, 991
FauI CCCGC 3 cut(s) 63, 66, 140
FbaI TGATCA 1 cut(s) 378
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 8 cut(s) 136, 139, 142, 145, 174, 337, 816, 1011
FokI GGATG 3 cut(s) 38, 699, 1123
Fsp4HI GCNGC 8 cut(s) 136, 139, 142, 145, 174, 337, 816, 1011
FspBI CTAG 1 cut(s) 429
GlaI GCGC 2 cut(s) 325, 1092
GluI GCNGC 8 cut(s) 136, 139, 142, 145, 174, 337, 816, 1011
GsuI CTGGAG 1 cut(s) 600
HaeII RGCGCY 1 cut(s) 327
HaeIII GGCC 7 cut(s) 38, 77, 173, 239, 246, 827, 859
HapII CCGG 5 cut(s) 255, 280, 350, 357, 707
HgaI GACGC 2 cut(s) 624, 1002
HhaI GCGC 2 cut(s) 326, 1093
Hin1I GRCGYC 1 cut(s) 635
Hin1II CATG 8 cut(s) 392, 447, 485, 506, 530, 591, 956, 995
Hin6I GCGC 2 cut(s) 324, 1091
HinP1I GCGC 2 cut(s) 324, 1091
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 3 cut(s) 155, 167, 1047
HpaII CCGG 5 cut(s) 255, 280, 350, 357, 707
HphI GGTGA 1 cut(s) 272
Hpy166II GTNNAC 3 cut(s) 6, 94, 639
Hpy188I TCNGA 6 cut(s) 154, 166, 224, 838, 872, 1018
Hpy188III TCNNGA 2 cut(s) 290, 804
Hpy8I GTNNAC 3 cut(s) 6, 94, 639
Hpy99I CGWCG 3 cut(s) 10, 272, 291
HpyAV CCTTC 6 cut(s) 257, 307, 525, 625, 662, 949
HpyCH4III ACNGT 3 cut(s) 100, 311, 934
HpyCH4IV ACGT 1 cut(s) 1078
HpyCH4V TGCA 7 cut(s) 447, 464, 502, 779, 815, 1054, 1157
HpyF10VI GCNNNNNNNGC 6 cut(s) 74, 141, 144, 425, 703, 824
HpySE526I ACGT 1 cut(s) 1078
Hsp92I GRCGYC 1 cut(s) 635
Hsp92II CATG 8 cut(s) 392, 447, 485, 506, 530, 591, 956, 995
HspAI GCGC 2 cut(s) 324, 1091
KroI GCCGGC 1 cut(s) 706
KroNI GCCGGC 1 cut(s) 708
Ksp22I TGATCA 1 cut(s) 378
Kzo9I GATC 6 cut(s) 378, 808, 838, 976, 994, 1086
LmnI GCTCC 7 cut(s) 19, 63, 117, 344, 617, 739, 760
Lsp1109I GCAGC 3 cut(s) 131, 827, 1022
LweI GCATC 2 cut(s) 57, 900
MaeI CTAG 1 cut(s) 429
MaeII ACGT 1 cut(s) 1078
MaeIII GTNAC 5 cut(s) 260, 602, 928, 970, 1144
MalI GATC 6 cut(s) 380, 810, 840, 978, 996, 1088
MbiI CCGCTC 2 cut(s) 58, 339
MboI GATC 6 cut(s) 378, 808, 838, 976, 994, 1086
MboII GAAGA 8 cut(s) 94, 112, 423, 571, 668, 941, 1094, 1151
MfeI CAATTG 1 cut(s) 717
MflI RGATCY 2 cut(s) 808, 976
MlsI TGGCCA 1 cut(s) 827
MluCI AATT 4 cut(s) 372, 651, 717, 792
MluNI TGGCCA 1 cut(s) 827
MlyI GAGTC 1 cut(s) 164
Mox20I TGGCCA 1 cut(s) 827
MroNI GCCGGC 1 cut(s) 706
MscI TGGCCA 1 cut(s) 827
MseI TTAA 3 cut(s) 702, 771, 921
MslI CAYNNNNRTG 2 cut(s) 255, 507
Msp20I TGGCCA 1 cut(s) 827
MspA1I CMGCKG 2 cut(s) 144, 408
MspI CCGG 5 cut(s) 255, 280, 350, 357, 707
MspR9I CCNGG 3 cut(s) 343, 579, 845
MunI CAATTG 1 cut(s) 717
MvaI CCWGG 3 cut(s) 343, 579, 845
MvnI CGCG 2 cut(s) 291, 1091
MwoI GCNNNNNNNGC 6 cut(s) 74, 141, 144, 425, 703, 824
NaeI GCCGGC 1 cut(s) 708
NdeII GATC 6 cut(s) 378, 808, 838, 976, 994, 1086
NgoMIV GCCGGC 1 cut(s) 706
NlaIII CATG 8 cut(s) 392, 447, 485, 506, 530, 591, 956, 995
NlaIV GGNNCC 4 cut(s) 37, 284, 735, 978
NmeAIII GCCGAG 1 cut(s) 265
NmuCI GTSAC 4 cut(s) 260, 602, 928, 970
NruI TCGCGA 1 cut(s) 291
OliI CACNNNNGTG 1 cut(s) 255
PcsI WCGNNNNNNNCGW 1 cut(s) 96
PdiI GCCGGC 1 cut(s) 708
PfeI GAWTC 2 cut(s) 167, 1047
PflMI CCANNNNNTGG 1 cut(s) 722
PinAI ACCGGT 1 cut(s) 254
PkrI GCNGC 8 cut(s) 137, 140, 143, 146, 175, 338, 817, 1012
PleI GAGTC 1 cut(s) 163
PpsI GAGTC 1 cut(s) 163
Psp6I CCWGG 3 cut(s) 341, 577, 843
PspGI CCWGG 3 cut(s) 341, 577, 843
PspN4I GGNNCC 4 cut(s) 37, 284, 735, 978
PspPI GGNCC 4 cut(s) 36, 75, 244, 857
PstI CTGCAG 1 cut(s) 817
PsuI RGATCY 2 cut(s) 808, 976
RruI TCGCGA 1 cut(s) 291
RsaI GTAC 3 cut(s) 585, 833, 936
RsaNI GTAC 3 cut(s) 584, 832, 935
RseI CAYNNNNRTG 2 cut(s) 255, 507
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 3 cut(s) 702, 771, 921
SatI GCNGC 8 cut(s) 136, 139, 142, 145, 174, 337, 816, 1011
Sau3AI GATC 6 cut(s) 378, 808, 838, 976, 994, 1086
Sau96I GGNCC 4 cut(s) 36, 75, 244, 857
ScaI AGTACT 1 cut(s) 833
SchI GAGTC 1 cut(s) 164
ScrFI CCNGG 3 cut(s) 343, 579, 845
SfaNI GCATC 2 cut(s) 57, 900
SfcI CTRYAG 1 cut(s) 813
SgrAI CRCCGGYG 1 cut(s) 254
SmiMI CAYNNNNRTG 2 cut(s) 255, 507
SmlI CTYRAG 1 cut(s) 62
SmoI CTYRAG 1 cut(s) 62
Sse9I AATT 4 cut(s) 372, 651, 717, 792
SspMI CTAG 1 cut(s) 429
StyD4I CCNGG 3 cut(s) 341, 577, 843
StyI CCWWGG 1 cut(s) 365
TaaI ACNGT 3 cut(s) 100, 311, 934
TaiI ACGT 1 cut(s) 1081
TaqI TCGA 2 cut(s) 5, 1104
TasI AATT 4 cut(s) 372, 651, 717, 792
TatI WGTACW 1 cut(s) 831
TauI GCSGC 5 cut(s) 138, 141, 144, 176, 339
TfiI GAWTC 2 cut(s) 167, 1047
Tru1I TTAA 3 cut(s) 702, 771, 921
Tru9I TTAA 3 cut(s) 702, 771, 921
TscAI CASTG 2 cut(s) 496, 825
TseFI GTSAC 4 cut(s) 260, 602, 928, 970
TseI GCWGC 3 cut(s) 144, 815, 1010
Tsp45I GTSAC 4 cut(s) 260, 602, 928, 970
TspDTI ATGAA 2 cut(s) 414, 941
TspGWI ACGGA 3 cut(s) 275, 285, 989
TspRI CASTG 2 cut(s) 496, 825
Van91I CCANNNNNTGG 1 cut(s) 722
XapI RAATTY 1 cut(s) 792
XmiI GTMKAC 1 cut(s) 5
XspI CTAG 1 cut(s) 429
ZrmI AGTACT 1 cut(s) 833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.