RLG00000010791

Monoglyceride

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013002
Physical Location & Seq
Forward (+)
4390967 .. 4393273
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010791

Sequence Viewer

Length: 1260 bp
ATGTTTCCGCTGCCGTTTCGCTTAGATTCTCCGACCCCTTTCGCGTTTCGCCGCCGTGCCTCACCTCCTCTCTCTCTCTCTCTCTCTCCACCGTCGATTTCCGCGGCCATGTCGACGGCGGAGATGGAGCAACTGACCTCGGGAGCGAGCAACCGCATCATCCCGCTCCTCAAGGCCCTGAGGACCTCCCTCCTCTTCGTCCACACCGTTTTCCTCTCGCTACTCCTCTTCGTCTTCCGCCGCCGCCGCCGATGCCTTTCTGAATCGCTCACGGAGTCGACGGCCAAGAAGCGGAAATCGGCGTGGAGGAGAGAGGAGGATGACACTTTCAGACGGAGAGCTTTGGCCGAGGAACTCCACACCGGAGACGGAGACTGCCGGTTCCGTCGCGACACCTTCTTGTTCTACGGTGTAAGGCAAAGCGCCTTATTCTGCCGGTCATGGTTTCCGGTCTCTGGTAATCCAAGGGCTATTTTGGTCATCATACATGGGCTGAATGAACACAGTGGAAGATATGCTGATTTTGCGAGGCAACTAACCTCATGCAACTTTGGGGTTTATGCAATGGACTGGATAGGTCATGGTGGCAGTGATGGATTGCATGGCTATGTTCCTTCACTTGACCATGTTGTTGCTGATACTGGGGATTTCTTGGAAAAGATTAGATTAGAGAACCCTGGAGCACCGTGCTTCCTCTTTGGTCACTCAACTGGAGGAGCTGTGGTTTTGAAGGCGGCAACCTATGCTAAAATTGAAGAAAAGGTGGAAGGTATAATACTGACCTCACCAGCTTTGCGTGTTAAGCCAGCACATCCAATTGTTGGGGCTGTGGCTCCACTTGTTTCTTTGGTCGCTCCCAAGTTCCAGTTTAAAGGTGCAAACAAAAGGGGAATTCCAGTATCTAGAGATCCTGCAGCACTGTTAGCCAAGTATTCCGATCCCCTGGTCTATACAGGGCCAATAAGGGTCAGAACAGGTTATGAGATATTGCGTATCTCTGCTCACTTGACGAGAAACTTTAAGTTTGTGACTGTACCCTTCTTTGTTCTCCATGGGACTGCTGATAGAGTCACGGATCCTCTGGCCTCTCAGGATCTGTACACAGAGGCAGCGTCTGAGTTCAAAGACATAAAGCTCTATGATGGTTTCTTGCACGATCTCCTGTTTGAGCCTGAACGCGAAGAGATTGCGCAGGATATAATTGACTGGATGGAGAAGCGATTAGGTGCTGGCCTTGAAGACGTTACCAACTTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00561 ko01100 ko04714 ko04723 ko04923 map00561 map01100 map04714 map04723 map04923
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

420

Amino Acids

46.77

Weight (kDa)

9.12

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 154 - 392 8e-75 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 158 - 372 9.5e-15 alpha/beta hydrolase fold
Abhydrolase_6 PF12697 159 - 366 7.4e-16 Alpha/beta hydrolase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013002)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G11650 AT5G11650
fragaria_vesca FvH4_2g35400
malus_domestica MD08G1112500.v1.1 MD15G1091700.v1.1
prunus_persica Prupe.1G446700_v2.0.a1
pyrus_communis pycom08g09340 pycom15g08550
rosa_chinensis RchiOBHm_Chr6g0306501
rosa_laevigata RLG00000010791
rosa_multiflora Rmu_sc0002971.1_g000010
rosa_roxburghii Rroxscaffold_7G00161720
rosa_rugosa Rorug06G0350000
rosa_samantha Rh6AG462100 Rh6BG438700 Rh6CG476400 Rh6DG463100
rosa_wichuraiana Rw6G040280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1191
AccB7I CCANNNNNTGG 1 cut(s) 821
AccBSI CCGCTC 1 cut(s) 166
AccI GTMKAC 2 cut(s) 113, 278
AccII CGCG 4 cut(s) 44, 104, 390, 1179
AclWI GGATC 5 cut(s) 902, 932, 1070, 1083, 1101
AcoI YGGCCR 3 cut(s) 105, 282, 345
AcsI RAATTY 1 cut(s) 891
AfaI GTAC 2 cut(s) 1035, 1100
AfiI CCNNNNNNNGG 3 cut(s) 291, 455, 821
AgsI TTSAA 4 cut(s) 730, 755, 1123, 1238
AjnI CCWGG 2 cut(s) 676, 942
AluBI AGCT 4 cut(s) 341, 719, 791, 1135
AluI AGCT 4 cut(s) 341, 719, 791, 1135
Alw21I GWGCWC 1 cut(s) 685
Alw26I GTCTC 3 cut(s) 360, 366, 457
AlwI GGATC 5 cut(s) 902, 932, 1070, 1083, 1101
AlwNI CAGNNNCTG 2 cut(s) 1096, 1115
Ama87I CYCGRG 1 cut(s) 139
AoxI GGCC 7 cut(s) 105, 174, 282, 345, 956, 1083, 1231
ApeKI GCWGC 3 cut(s) 10, 914, 1109
ApoI RAATTY 1 cut(s) 891
AspLEI GCGC 2 cut(s) 425, 1192
AspS9I GGNCC 3 cut(s) 175, 183, 956
AsuHPI GGTGA 2 cut(s) 54, 777
AvaI CYCGRG 1 cut(s) 139
AvaII GGWCC 1 cut(s) 183
AxyI CCTNAGG 1 cut(s) 179
BamHI GGATCC 1 cut(s) 1075
BarI GAAGNNNNNNTAC 2 cut(s) 759, 791
BbsI GAAGAC 2 cut(s) 226, 1245
Bbv12I GWGCWC 1 cut(s) 685
BbvI GCAGC 2 cut(s) 926, 1121
BccI CCATC 4 cut(s) 118, 587, 1136, 1204
BceAI ACGGC 3 cut(s) 39, 132, 297
BcgI CGANNNNNNTGC 2 cut(s) 1169, 1203
BciT130I CCWGG 2 cut(s) 678, 944
BcoDI GTCTC 3 cut(s) 360, 366, 457
BfaI CTAG 1 cut(s) 903
BfmI CTRYAG 1 cut(s) 912
BfoI RGCGCY 1 cut(s) 426
BisI GCNGC 9 cut(s) 11, 52, 105, 241, 244, 247, 735, 915, 1110
BlsI GCNGC 9 cut(s) 12, 53, 106, 242, 245, 248, 736, 916, 1111
Bme1390I CCNGG 2 cut(s) 678, 944
Bme18I GGWCC 1 cut(s) 183
BmeT110I CYCGRG 1 cut(s) 139
BmgT120I GGNCC 3 cut(s) 175, 183, 956
BmiI GGNNCC 3 cut(s) 383, 834, 1077
BmrFI CCNGG 2 cut(s) 678, 944
BmrI ACTGGG 1 cut(s) 651
BmsI GCATC 2 cut(s) 165, 242
BmuI ACTGGG 1 cut(s) 651
BpiI GAAGAC 2 cut(s) 226, 1245
BpmI CTGGAG 2 cut(s) 699, 732
BpuEI CTTGAG 1 cut(s) 155
BsaI GGTCTC 1 cut(s) 457
BsaJI CCNNGG 7 cut(s) 102, 138, 348, 464, 676, 942, 1051
BsaWI WCCGGW 2 cut(s) 362, 448
BsaXI ACNNNNNCTCC 4 cut(s) 705, 708, 735, 738
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 455, 821
Bse118I RCCGGY 2 cut(s) 378, 435
Bse1I ACTGG 6 cut(s) 575, 646, 715, 865, 896, 1211
Bse21I CCTNAGG 1 cut(s) 179
Bse3DI GCAATG 1 cut(s) 570
BseBI CCWGG 2 cut(s) 678, 944
BseDI CCNNGG 7 cut(s) 102, 138, 348, 464, 676, 942, 1051
BseGI GGATG 4 cut(s) 159, 325, 811, 1215
BseLI CCNNNNNNNGG 3 cut(s) 291, 455, 821
BseMI GCAATG 1 cut(s) 570
BseMII CTCAG 3 cut(s) 170, 1103, 1107
BseNI ACTGG 6 cut(s) 575, 646, 715, 865, 896, 1211
BseRI GAGGAG 7 cut(s) 57, 158, 182, 215, 322, 329, 729
BseXI GCAGC 2 cut(s) 926, 1121
Bsh1236I CGCG 4 cut(s) 44, 104, 390, 1179
BshFI GGCC 7 cut(s) 107, 176, 284, 347, 958, 1085, 1233
BsiHKAI GWGCWC 1 cut(s) 685
BsiHKCI CYCGRG 1 cut(s) 139
BsiSI CCGG 4 cut(s) 363, 379, 436, 449
BslFI GGGAC 1 cut(s) 1069
BslI CCNNNNNNNGG 3 cut(s) 291, 455, 821
BsmAI GTCTC 3 cut(s) 360, 366, 457
BsmBI CGTCTC 1 cut(s) 360
BsmFI GGGAC 1 cut(s) 1069
BsnI GGCC 7 cut(s) 107, 176, 284, 347, 958, 1085, 1233
Bso31I GGTCTC 1 cut(s) 457
BsoBI CYCGRG 1 cut(s) 139
Bsp1286I GDGCHC 1 cut(s) 685
Bsp1407I TGTACA 1 cut(s) 1098
Bsp143I GATC 5 cut(s) 907, 937, 1075, 1093, 1156
Bsp19I CCATGG 1 cut(s) 1051
Bsp68I TCGCGA 1 cut(s) 390
BspANI GGCC 7 cut(s) 107, 176, 284, 347, 958, 1085, 1233
BspCNI CTCAG 3 cut(s) 171, 1102, 1108
BspFNI CGCG 4 cut(s) 44, 104, 390, 1179
BspLI GGNNCC 3 cut(s) 383, 834, 1077
BspMAI CTGCAG 1 cut(s) 916
BspPI GGATC 5 cut(s) 902, 932, 1070, 1083, 1101
BspTNI GGTCTC 1 cut(s) 457
BsrBI CCGCTC 1 cut(s) 166
BsrDI GCAATG 1 cut(s) 570
BsrFI RCCGGY 2 cut(s) 378, 435
BsrGI TGTACA 1 cut(s) 1098
BsrI ACTGG 6 cut(s) 575, 646, 715, 865, 896, 1211
BssAI RCCGGY 2 cut(s) 378, 435
BssECI CCNNGG 7 cut(s) 102, 138, 348, 464, 676, 942, 1051
BssMI GATC 5 cut(s) 907, 937, 1075, 1093, 1156
BssT1I CCWWGG 2 cut(s) 464, 1051
Bst2UI CCWGG 2 cut(s) 678, 944
Bst4CI ACNGT 7 cut(s) 93, 208, 410, 506, 687, 921, 1033
Bst6I CTCTTC 3 cut(s) 200, 233, 1176
BstAPI GCANNNNNTGC 1 cut(s) 743
BstAUI TGTACA 1 cut(s) 1098
BstC8I GCNNGC 3 cut(s) 148, 807, 1231
BstDEI CTNAG 4 cut(s) 22, 179, 1089, 1116
BstDSI CCRYGG 2 cut(s) 102, 1051
BstF5I GGATG 4 cut(s) 159, 325, 811, 1215
BstFNI CGCG 4 cut(s) 44, 104, 390, 1179
BstH2I RGCGCY 1 cut(s) 426
BstHHI GCGC 2 cut(s) 425, 1192
BstKTI GATC 5 cut(s) 910, 940, 1078, 1096, 1159
BstMAI GTCTC 3 cut(s) 360, 366, 457
BstMBI GATC 5 cut(s) 907, 937, 1075, 1093, 1156
BstMWI GCNNNNNNNGC 6 cut(s) 246, 252, 524, 743, 802, 923
BstNI CCWGG 2 cut(s) 678, 944
BstSCI CCNGG 2 cut(s) 676, 942
BstSFI CTRYAG 1 cut(s) 912
BstUI CGCG 4 cut(s) 44, 104, 390, 1179
BstV1I GCAGC 2 cut(s) 926, 1121
BstV2I GAAGAC 2 cut(s) 226, 1245
BstX2I RGATCY 3 cut(s) 907, 1075, 1093
BstYI RGATCY 3 cut(s) 907, 1075, 1093
Bsu36I CCTNAGG 1 cut(s) 179
BsuRI GGCC 7 cut(s) 107, 176, 284, 347, 958, 1085, 1233
BtgI CCRYGG 2 cut(s) 102, 1051
BtsCI GGATG 4 cut(s) 159, 325, 811, 1215
BtsI GCAGTG 1 cut(s) 595
BtsIMutI CAGTG 3 cut(s) 511, 595, 917
BtuMI TCGCGA 1 cut(s) 390
Cac8I GCNNGC 3 cut(s) 148, 807, 1231
CaiI CAGNNNCTG 2 cut(s) 1096, 1115
CfoI GCGC 2 cut(s) 425, 1192
Cfr10I RCCGGY 2 cut(s) 378, 435
Cfr13I GGNCC 3 cut(s) 175, 183, 956
Cfr42I CCGCGG 1 cut(s) 105
CseI GACGC 1 cut(s) 1101
Csp6I GTAC 2 cut(s) 1034, 1099
CviAII CATG 8 cut(s) 109, 441, 488, 543, 581, 602, 626, 1052
CviQI GTAC 2 cut(s) 1034, 1099
DdeI CTNAG 4 cut(s) 22, 179, 1089, 1116
DpnI GATC 5 cut(s) 909, 939, 1077, 1095, 1158
DpnII GATC 5 cut(s) 907, 937, 1075, 1093, 1156
DraI TTTAAA 1 cut(s) 871
EaeI YGGCCR 3 cut(s) 105, 282, 345
Eam1104I CTCTTC 3 cut(s) 200, 233, 1176
EarI CTCTTC 3 cut(s) 200, 233, 1176
EciI GGCGGA 2 cut(s) 134, 227
Eco130I CCWWGG 2 cut(s) 464, 1051
Eco31I GGTCTC 1 cut(s) 457
Eco47I GGWCC 1 cut(s) 183
Eco81I CCTNAGG 1 cut(s) 179
Eco88I CYCGRG 1 cut(s) 139
EcoO109I RGGNCCY 2 cut(s) 175, 183
EcoRI GAATTC 1 cut(s) 891
EcoRII CCWGG 2 cut(s) 676, 942
EcoT14I CCWWGG 2 cut(s) 464, 1051
ErhI CCWWGG 2 cut(s) 464, 1051
Esp3I CGTCTC 1 cut(s) 360
FaeI CATG 8 cut(s) 112, 444, 491, 546, 584, 605, 629, 1055
FaqI GGGAC 1 cut(s) 1069
FatI CATG 8 cut(s) 108, 440, 487, 542, 580, 601, 625, 1051
FauI CCCGC 1 cut(s) 171
FblI GTMKAC 2 cut(s) 113, 278
Fnu4HI GCNGC 9 cut(s) 11, 52, 105, 241, 244, 247, 735, 915, 1110
FokI GGATG 4 cut(s) 146, 332, 798, 1222
Fsp4HI GCNGC 9 cut(s) 11, 52, 105, 241, 244, 247, 735, 915, 1110
FspBI CTAG 1 cut(s) 903
FspI TGCGCA 1 cut(s) 1191
GlaI GCGC 2 cut(s) 424, 1191
GluI GCNGC 9 cut(s) 11, 52, 105, 241, 244, 247, 735, 915, 1110
GsuI CTGGAG 2 cut(s) 699, 732
HaeII RGCGCY 1 cut(s) 426
HaeIII GGCC 7 cut(s) 107, 176, 284, 347, 958, 1085, 1233
HapII CCGG 4 cut(s) 363, 379, 436, 449
HgaI GACGC 1 cut(s) 1101
HhaI GCGC 2 cut(s) 425, 1192
Hin1II CATG 8 cut(s) 112, 444, 491, 546, 584, 605, 629, 1055
Hin6I GCGC 2 cut(s) 423, 1190
HinP1I GCGC 2 cut(s) 423, 1190
HincII GTYRAC 2 cut(s) 114, 279
HindII GTYRAC 2 cut(s) 114, 279
HinfI GANTC 4 cut(s) 26, 263, 275, 1068
HpaII CCGG 4 cut(s) 363, 379, 436, 449
HphI GGTGA 2 cut(s) 54, 777
Hpy166II GTNNAC 4 cut(s) 114, 202, 279, 1101
Hpy188I TCNGA 6 cut(s) 33, 262, 332, 937, 971, 1117
Hpy188III TCNNGA 4 cut(s) 141, 389, 903, 1091
Hpy8I GTNNAC 4 cut(s) 114, 202, 279, 1101
Hpy99I CGWCG 4 cut(s) 97, 118, 283, 390
HpyAV CCTTC 5 cut(s) 406, 624, 724, 761, 1048
HpyCH4III ACNGT 7 cut(s) 93, 208, 410, 506, 687, 921, 1033
HpyCH4IV ACGT 1 cut(s) 1242
HpyCH4V TGCA 7 cut(s) 546, 563, 601, 878, 914, 1153, 1256
HpyF10VI GCNNNNNNNGC 6 cut(s) 246, 252, 524, 743, 802, 923
HpyF3I CTNAG 4 cut(s) 22, 179, 1089, 1116
HpySE526I ACGT 1 cut(s) 1242
Hsp92II CATG 8 cut(s) 112, 444, 491, 546, 584, 605, 629, 1055
HspAI GCGC 2 cut(s) 423, 1190
KspI CCGCGG 1 cut(s) 105
Kzo9I GATC 5 cut(s) 907, 937, 1075, 1093, 1156
LmnI GCTCC 7 cut(s) 127, 143, 171, 680, 716, 838, 859
Lsp1109I GCAGC 2 cut(s) 926, 1121
LweI GCATC 2 cut(s) 165, 242
MaeI CTAG 1 cut(s) 903
MaeII ACGT 1 cut(s) 1242
MaeIII GTNAC 4 cut(s) 701, 1027, 1069, 1243
MalI GATC 5 cut(s) 909, 939, 1077, 1095, 1158
MbiI CCGCTC 1 cut(s) 166
MboI GATC 5 cut(s) 907, 937, 1075, 1093, 1156
MboII GAAGA 7 cut(s) 187, 220, 226, 522, 767, 1193, 1250
MfeI CAATTG 1 cut(s) 816
MflI RGATCY 3 cut(s) 907, 1075, 1093
MhlI GDGCHC 1 cut(s) 685
MluCI AATT 4 cut(s) 750, 816, 891, 1200
MlyI GAGTC 2 cut(s) 284, 1077
MmeI TCCRAC 1 cut(s) 56
MseI TTAA 3 cut(s) 801, 870, 1020
MslI CAYNNNNRTG 1 cut(s) 606
MspA1I CMGCKG 2 cut(s) 10, 104
MspI CCGG 4 cut(s) 363, 379, 436, 449
MspR9I CCNGG 2 cut(s) 678, 944
MunI CAATTG 1 cut(s) 816
MvaI CCWGG 2 cut(s) 678, 944
MvnI CGCG 4 cut(s) 44, 104, 390, 1179
MwoI GCNNNNNNNGC 6 cut(s) 246, 252, 524, 743, 802, 923
NcoI CCATGG 1 cut(s) 1051
NdeII GATC 5 cut(s) 907, 937, 1075, 1093, 1156
NlaIII CATG 8 cut(s) 112, 444, 491, 546, 584, 605, 629, 1055
NlaIV GGNNCC 3 cut(s) 383, 834, 1077
NmeAIII GCCGAG 1 cut(s) 373
NmuCI GTSAC 3 cut(s) 701, 1027, 1069
NruI TCGCGA 1 cut(s) 390
NsbI TGCGCA 1 cut(s) 1191
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 2 cut(s) 26, 263
PflMI CCANNNNNTGG 1 cut(s) 821
PkrI GCNGC 9 cut(s) 12, 53, 106, 242, 245, 248, 736, 916, 1111
PleI GAGTC 2 cut(s) 283, 1076
PpsI GAGTC 2 cut(s) 283, 1076
PpuMI RGGWCCY 1 cut(s) 183
Psp5II RGGWCCY 1 cut(s) 183
Psp6I CCWGG 2 cut(s) 676, 942
PspGI CCWGG 2 cut(s) 676, 942
PspN4I GGNNCC 3 cut(s) 383, 834, 1077
PspPI GGNCC 3 cut(s) 175, 183, 956
PspPPI RGGWCCY 1 cut(s) 183
PstI CTGCAG 1 cut(s) 916
PstNI CAGNNNCTG 2 cut(s) 1096, 1115
PsuI RGATCY 3 cut(s) 907, 1075, 1093
RruI TCGCGA 1 cut(s) 390
RsaI GTAC 2 cut(s) 1035, 1100
RsaNI GTAC 2 cut(s) 1034, 1099
RseI CAYNNNNRTG 1 cut(s) 606
SacII CCGCGG 1 cut(s) 105
SalI GTCGAC 2 cut(s) 112, 277
SaqAI TTAA 3 cut(s) 801, 870, 1020
SatI GCNGC 9 cut(s) 11, 52, 105, 241, 244, 247, 735, 915, 1110
Sau3AI GATC 5 cut(s) 907, 937, 1075, 1093, 1156
Sau96I GGNCC 3 cut(s) 175, 183, 956
SchI GAGTC 2 cut(s) 284, 1077
ScrFI CCNGG 2 cut(s) 678, 944
SduI GDGCHC 1 cut(s) 685
SfaNI GCATC 2 cut(s) 165, 242
SfcI CTRYAG 1 cut(s) 912
Sfr303I CCGCGG 1 cut(s) 105
SgrBI CCGCGG 1 cut(s) 105
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 606
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse9I AATT 4 cut(s) 750, 816, 891, 1200
SspMI CTAG 1 cut(s) 903
StyD4I CCNGG 2 cut(s) 676, 942
StyI CCWWGG 2 cut(s) 464, 1051
TaaI ACNGT 7 cut(s) 93, 208, 410, 506, 687, 921, 1033
TaiI ACGT 1 cut(s) 1245
TaqI TCGA 3 cut(s) 95, 113, 278
TasI AATT 4 cut(s) 750, 816, 891, 1200
TatI WGTACW 1 cut(s) 1098
TauI GCSGC 6 cut(s) 54, 107, 243, 246, 249, 737
TfiI GAWTC 2 cut(s) 26, 263
Tru1I TTAA 3 cut(s) 801, 870, 1020
Tru9I TTAA 3 cut(s) 801, 870, 1020
TscAI CASTG 3 cut(s) 511, 595, 924
TseFI GTSAC 3 cut(s) 701, 1027, 1069
TseI GCWGC 3 cut(s) 10, 914, 1109
Tsp45I GTSAC 3 cut(s) 701, 1027, 1069
TspDTI ATGAA 1 cut(s) 513
TspGWI ACGGA 5 cut(s) 287, 349, 374, 384, 1088
TspRI CASTG 3 cut(s) 511, 595, 924
Van91I CCANNNNNTGG 1 cut(s) 821
VpaK11BI GGWCC 1 cut(s) 183
XapI RAATTY 1 cut(s) 891
XbaI TCTAGA 1 cut(s) 902
XmiI GTMKAC 2 cut(s) 113, 278
XspI CTAG 1 cut(s) 903
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.