AT5G12300

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012150
Physical Location & Seq
Reverse (-)
3978281 .. 3980021
1741 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G12300.1

Sequence Viewer

Length: 1125 bp
ATGGAATCTCTGAAACAAGCTTCTTCTGCTTCTGGGTTCTGCTACCACCAAGGCTTCTCAGGTGTTCTCCAAGTGTATGTTCACAACGCAAGAAACATCAACAACATCTGCATCTACGATAACCAAGACGTCTACGCCAAATTCTCTCTCACATACAATCCTGACGATACCATCTCCACCAGAATCATCCATAGAGCTGGCAAAAACCCTGAATTCAACCAGAAGCTTATGATCGATGTAACTCAGATCGATGCTCATGCTGCAGTTCTCAAATGTGAGATCTGGATGATGAGCAGAGCCAGACATTACATGGAAGATCAGCTTCTTGGTTTCGCTCTTGTCCCAATCTCAGACATCATAGGTCAAGACAGTGTAACTCAAGACTACAGTCTCTCCTCCACTGATCTCTTCCATTCCCCTGCTGGTACCGTGAAGCTCACGCTTTCCATTGTTAACCCTTCTTCTACTTCTTCATCTAATCCCAAGATTAACACCACTTCGATTTCTTCCGAGGTCGTGTTGCTTGACCCTCAAGTCTCTGAAACAGTAGATTACACGAGGATTGAGTTCCCTGACATCAATGTTGCTAACGAGAACAAACAGATGGTTACAGAGTACTTCAATGAATCCGGAACCGGTTCATTTCTCTGTCTCGGCTCAACACATGGACCAGAAACGGACATAACAATGGTGTGCTTGGAAGAAAAAGAGCTTTATGGGAATGGAAGCTTCATGGCTTCTTCTTCAACAACAACAAGCTTAAGCGACGAAAAGAACACAGCAGATTCAAACGAGAAAGAGAATCGAGAGATAACAGAAGTATCAAGAAGAAGAAGCAAAGAAGGTGAAGAAGAAGAGGAAGAGAATAAGATGAATGAAGAAACAACAATGCAGAAACAGATAGCAGAGATGTATATGAGAAGTATGCAACAGTTCACTGAGTCTTTGGCAAAGATGAAACTTCCCATGGATCTTCATAATAAACCACACGAGGAAGATCACAGTAACAATAACAACAACACTGCAACACAGATTCAGAATCAGAATAATAATGCTAATAATAATGGTATGGAGAAGAAAAAAGAAGGGTCTCGTGTGTTTTACGGTAGCAGGGCATTCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

374

Amino Acids

42.1

Weight (kDa)

4.92

Isoelectric Point (pI)

53.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 21 - 130 1.1e-09 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12300
fragaria_vesca FvH4_3g27890 FvH4_3g27890 FvH4_3g27890
malus_domestica MD03G1166500.v1.1
prunus_persica Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1
pyrus_communis pycom03g12210
rosa_chinensis RchiOBHm_Chr5g0050621
rosa_laevigata RLG00000034712
rosa_multiflora Rmu_ssc0000350.1_g000030
rosa_roxburghii Rroxscaffold_1G00030180
rosa_rugosa Rorug05G0262100
rosa_samantha Rh5AG334400 Rh5BG343800 Rh5CG371600 Rh5DG356800
rosa_wichuraiana Rw5G031550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 533
AatII GACGTC 1 cut(s) 132
Acc65I GGTACC 1 cut(s) 425
AccB1I GGYRCC 1 cut(s) 425
AccI GTMKAC 1 cut(s) 132
AccIII TCCGGA 1 cut(s) 629
AclWI GGATC 1 cut(s) 978
AcsI RAATTY 2 cut(s) 140, 212
AcyI GRCGYC 1 cut(s) 129
AfaI GTAC 2 cut(s) 427, 617
AflII CTTAAG 1 cut(s) 760
AgeI ACCGGT 1 cut(s) 635
AgsI TTSAA 4 cut(s) 217, 622, 747, 789
AjuI GAANNNNNNNTTGG 2 cut(s) 63, 95
AluBI AGCT 8 cut(s) 20, 197, 226, 322, 436, 712, 729, 759
AluI AGCT 8 cut(s) 20, 197, 226, 322, 436, 712, 729, 759
Alw26I GTCTC 4 cut(s) 395, 541, 656, 1095
AlwI GGATC 1 cut(s) 978
Aor13HI TCCGGA 1 cut(s) 629
ApeKI GCWGC 1 cut(s) 260
ApoI RAATTY 2 cut(s) 140, 212
AsiGI ACCGGT 1 cut(s) 635
Asp700I GAANNNNTTC 1 cut(s) 637
Asp718I GGTACC 1 cut(s) 425
AspS9I GGNCC 1 cut(s) 668
AsuHPI GGTGA 1 cut(s) 857
AvaII GGWCC 1 cut(s) 668
BaeI ACNNNNGTAYC 2 cut(s) 804, 837
BanI GGYRCC 1 cut(s) 425
BauI CACGAG 3 cut(s) 556, 989, 1092
BbvI GCAGC 1 cut(s) 247
BccI CCATC 2 cut(s) 179, 598
BcgI CGANNNNNNTGC 2 cut(s) 239, 273
BcoDI GTCTC 4 cut(s) 395, 541, 656, 1095
BfmI CTRYAG 2 cut(s) 261, 385
BfrI CTTAAG 1 cut(s) 760
BglII AGATCT 1 cut(s) 279
BisI GCNGC 1 cut(s) 261
BlsI GCNGC 1 cut(s) 262
BmcAI AGTACT 1 cut(s) 617
Bme18I GGWCC 1 cut(s) 668
BmgT120I GGNCC 1 cut(s) 668
BmiI GGNNCC 2 cut(s) 427, 634
BmsI GCATC 2 cut(s) 120, 241
BoxI GACNNNNGTC 1 cut(s) 387
BpuEI CTTGAG 2 cut(s) 363, 516
Bsa29I ATCGAT 2 cut(s) 234, 249
BsaHI GRCGYC 1 cut(s) 129
BsaI GGTCTC 1 cut(s) 1095
BsaJI CCNNGG 3 cut(s) 49, 510, 966
BsaWI WCCGGW 2 cut(s) 629, 635
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bse118I RCCGGY 1 cut(s) 635
BseAI TCCGGA 1 cut(s) 629
BseCI ATCGAT 2 cut(s) 234, 249
BseDI CCNNGG 3 cut(s) 49, 510, 966
BseGI GGATG 2 cut(s) 186, 291
BseMII CTCAG 4 cut(s) 72, 257, 363, 930
BseRI GAGGAG 1 cut(s) 385
BseXI GCAGC 1 cut(s) 247
BshNI GGYRCC 1 cut(s) 425
BshTI ACCGGT 1 cut(s) 635
BshVI ATCGAT 2 cut(s) 234, 249
BsiSI CCGG 2 cut(s) 630, 636
BslFI GGGAC 1 cut(s) 326
BsmAI GTCTC 4 cut(s) 395, 541, 656, 1095
BsmFI GGGAC 1 cut(s) 326
BsmI GAATGC 1 cut(s) 1115
Bso31I GGTCTC 1 cut(s) 1095
Bsp13I TCCGGA 1 cut(s) 629
Bsp143I GATC 7 cut(s) 231, 246, 279, 316, 403, 970, 997
Bsp19I CCATGG 1 cut(s) 966
BspCNI CTCAG 4 cut(s) 71, 256, 362, 931
BspDI ATCGAT 2 cut(s) 234, 249
BspEI TCCGGA 1 cut(s) 629
BspLI GGNNCC 2 cut(s) 427, 634
BspMAI CTGCAG 1 cut(s) 265
BspPI GGATC 1 cut(s) 978
BspT107I GGYRCC 1 cut(s) 425
BspTI CTTAAG 1 cut(s) 760
BspTNI GGTCTC 1 cut(s) 1095
BsrFI RCCGGY 1 cut(s) 635
BssAI RCCGGY 1 cut(s) 635
BssECI CCNNGG 3 cut(s) 49, 510, 966
BssMI GATC 7 cut(s) 231, 246, 279, 316, 403, 970, 997
BssNI GRCGYC 1 cut(s) 129
BssSI CACGAG 3 cut(s) 556, 989, 1092
BssT1I CCWWGG 2 cut(s) 49, 966
Bst2BI CACGAG 3 cut(s) 556, 989, 1092
Bst4CI ACNGT 7 cut(s) 371, 389, 430, 547, 933, 1004, 1106
Bst6I CTCTTC 3 cut(s) 413, 849, 855
BstACI GRCGYC 1 cut(s) 129
BstAFI CTTAAG 1 cut(s) 760
BstC8I GCNNGC 1 cut(s) 199
BstDEI CTNAG 4 cut(s) 58, 243, 349, 939
BstDSI CCRYGG 1 cut(s) 966
BstF5I GGATG 2 cut(s) 186, 291
BstKTI GATC 7 cut(s) 234, 249, 282, 319, 406, 973, 1000
BstMAI GTCTC 4 cut(s) 395, 541, 656, 1095
BstMBI GATC 7 cut(s) 231, 246, 279, 316, 403, 970, 997
BstMWI GCNNNNNNNGC 2 cut(s) 26, 260
BstPAI GACNNNNGTC 1 cut(s) 387
BstSFI CTRYAG 2 cut(s) 261, 385
BstV1I GCAGC 1 cut(s) 247
BstX2I RGATCY 2 cut(s) 279, 970
BstXI CCANNNNNNTGG 1 cut(s) 197
BstYI RGATCY 2 cut(s) 279, 970
Bsu15I ATCGAT 2 cut(s) 234, 249
BsuTUI ATCGAT 2 cut(s) 234, 249
BtgI CCRYGG 1 cut(s) 966
BtsCI GGATG 2 cut(s) 186, 291
BtsI GCAGTG 1 cut(s) 1020
BtsIMutI CAGTG 4 cut(s) 376, 399, 936, 1020
Cac8I GCNNGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 635
Cfr13I GGNCC 1 cut(s) 668
ClaI ATCGAT 2 cut(s) 234, 249
Csp6I GTAC 2 cut(s) 426, 616
CspAI ACCGGT 1 cut(s) 635
CviAII CATG 5 cut(s) 257, 310, 665, 733, 967
CviQI GTAC 2 cut(s) 426, 616
DdeI CTNAG 4 cut(s) 58, 243, 349, 939
DpnI GATC 7 cut(s) 233, 248, 281, 318, 405, 972, 999
DpnII GATC 7 cut(s) 231, 246, 279, 316, 403, 970, 997
DrdI GACNNNNNNGTC 1 cut(s) 533
DseDI GACNNNNNNGTC 1 cut(s) 533
Eam1104I CTCTTC 3 cut(s) 413, 849, 855
EarI CTCTTC 3 cut(s) 413, 849, 855
Eco130I CCWWGG 2 cut(s) 49, 966
Eco31I GGTCTC 1 cut(s) 1095
Eco47I GGWCC 1 cut(s) 668
EcoRI GAATTC 1 cut(s) 212
EcoT14I CCWWGG 2 cut(s) 49, 966
ErhI CCWWGG 2 cut(s) 49, 966
FaeI CATG 5 cut(s) 260, 313, 668, 736, 970
FalI AAGNNNNNCTT 2 cut(s) 306, 338
FaqI GGGAC 1 cut(s) 326
FatI CATG 5 cut(s) 256, 309, 664, 732, 966
FblI GTMKAC 1 cut(s) 132
Fnu4HI GCNGC 1 cut(s) 261
FokI GGATG 2 cut(s) 173, 298
Fsp4HI GCNGC 1 cut(s) 261
GluI GCNGC 1 cut(s) 261
HapII CCGG 2 cut(s) 630, 636
Hin1I GRCGYC 1 cut(s) 129
Hin1II CATG 5 cut(s) 260, 313, 668, 736, 970
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 4 cut(s) 18, 224, 727, 757
HinfI GANTC 8 cut(s) 5, 183, 626, 785, 802, 941, 1033, 1039
HpaI GTTAAC 1 cut(s) 454
HpaII CCGG 2 cut(s) 630, 636
HphI GGTGA 1 cut(s) 857
Hpy166II GTNNAC 4 cut(s) 82, 133, 454, 936
Hpy188I TCNGA 7 cut(s) 12, 246, 352, 511, 541, 1038, 1044
Hpy188III TCNNGA 7 cut(s) 161, 283, 365, 380, 630, 806, 825
Hpy8I GTNNAC 4 cut(s) 82, 133, 454, 936
Hpy99I CGWCG 1 cut(s) 770
HpyAV CCTTC 3 cut(s) 468, 836, 1079
HpyCH4III ACNGT 7 cut(s) 371, 389, 430, 547, 933, 1004, 1106
HpyCH4IV ACGT 1 cut(s) 129
HpyCH4V TGCA 5 cut(s) 111, 263, 892, 928, 1025
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 260
HpyF3I CTNAG 4 cut(s) 58, 243, 349, 939
HpySE526I ACGT 1 cut(s) 129
Hsp92I GRCGYC 1 cut(s) 129
Hsp92II CATG 5 cut(s) 260, 313, 668, 736, 970
Kpn2I TCCGGA 1 cut(s) 629
KpnI GGTACC 1 cut(s) 429
KspAI GTTAAC 1 cut(s) 454
Kzo9I GATC 7 cut(s) 231, 246, 279, 316, 403, 970, 997
Lsp1109I GCAGC 1 cut(s) 247
LweI GCATC 2 cut(s) 120, 241
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 4 cut(s) 238, 373, 607, 1004
MalI GATC 7 cut(s) 233, 248, 281, 318, 405, 972, 999
MboI GATC 7 cut(s) 231, 246, 279, 316, 403, 970, 997
MflI RGATCY 2 cut(s) 279, 970
MluCI AATT 2 cut(s) 140, 212
MlyI GAGTC 1 cut(s) 950
MnlI CCTC 6 cut(s) 406, 505, 540, 552, 850, 985
MroI TCCGGA 1 cut(s) 629
MroXI GAANNNNTTC 1 cut(s) 637
MseI TTAA 4 cut(s) 453, 489, 761, 1123
MslI CAYNNNNRTG 1 cut(s) 686
MspCI CTTAAG 1 cut(s) 760
MspI CCGG 2 cut(s) 630, 636
Mva1269I GAATGC 1 cut(s) 1115
MwoI GCNNNNNNNGC 2 cut(s) 26, 260
NcoI CCATGG 1 cut(s) 966
NdeII GATC 7 cut(s) 231, 246, 279, 316, 403, 970, 997
NlaIII CATG 5 cut(s) 260, 313, 668, 736, 970
NlaIV GGNNCC 2 cut(s) 427, 634
NmeAIII GCCGAG 1 cut(s) 633
PctI GAATGC 1 cut(s) 1115
PdmI GAANNNNTTC 1 cut(s) 637
PfeI GAWTC 7 cut(s) 5, 183, 626, 785, 802, 1033, 1039
PinAI ACCGGT 1 cut(s) 635
PkrI GCNGC 1 cut(s) 262
PleI GAGTC 1 cut(s) 949
PpsI GAGTC 1 cut(s) 949
PshAI GACNNNNGTC 1 cut(s) 387
PspN4I GGNNCC 2 cut(s) 427, 634
PspPI GGNCC 1 cut(s) 668
PstI CTGCAG 1 cut(s) 265
PsuI RGATCY 2 cut(s) 279, 970
RsaI GTAC 2 cut(s) 427, 617
RsaNI GTAC 2 cut(s) 426, 616
RseI CAYNNNNRTG 1 cut(s) 686
SaqAI TTAA 4 cut(s) 453, 489, 761, 1123
SatI GCNGC 1 cut(s) 261
Sau3AI GATC 7 cut(s) 231, 246, 279, 316, 403, 970, 997
Sau96I GGNCC 1 cut(s) 668
ScaI AGTACT 1 cut(s) 617
SchI GAGTC 1 cut(s) 950
SfaNI GCATC 2 cut(s) 120, 241
SfcI CTRYAG 2 cut(s) 261, 385
SinI GGWCC 1 cut(s) 668
SmiMI CAYNNNNRTG 1 cut(s) 686
SmlI CTYRAG 3 cut(s) 378, 531, 760
SmoI CTYRAG 3 cut(s) 378, 531, 760
Sse9I AATT 2 cut(s) 140, 212
StyI CCWWGG 2 cut(s) 49, 966
TaaI ACNGT 7 cut(s) 371, 389, 430, 547, 933, 1004, 1106
TaiI ACGT 1 cut(s) 132
TaqI TCGA 4 cut(s) 234, 249, 500, 805
TasI AATT 2 cut(s) 140, 212
TatI WGTACW 1 cut(s) 615
TfiI GAWTC 7 cut(s) 5, 183, 626, 785, 802, 1033, 1039
Tru1I TTAA 4 cut(s) 453, 489, 761, 1123
Tru9I TTAA 4 cut(s) 453, 489, 761, 1123
TscAI CASTG 4 cut(s) 376, 406, 943, 1027
TseI GCWGC 1 cut(s) 260
TspDTI ATGAA 8 cut(s) 462, 630, 639, 721, 887, 891, 965, 971
TspGWI ACGGA 1 cut(s) 692
TspRI CASTG 4 cut(s) 376, 406, 943, 1027
Vha464I CTTAAG 1 cut(s) 760
VpaK11BI GGWCC 1 cut(s) 668
XapI RAATTY 2 cut(s) 140, 212
XcmI CCANNNNNNNNNTGG 2 cut(s) 307, 419
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 637
ZraI GACGTC 1 cut(s) 130
ZrmI AGTACT 1 cut(s) 617
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.