Prupe.4G267000_v2.0.a1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0012150
Physical Location & Seq
Reverse (-)
20066778 .. 20069037
2260 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G267000.3

Sequence Viewer

Length: 1374 bp
ATGGAATCATTCAATCAGACAACTGGGTTCCGGTACAACCTGAATCCGAACACAATAGGTGATGTAAATGGTGATTCTGATCACTTTTCAGGGATTCTTGAAATCTATGCTCATCATGCTAGGAACATTCACAACATATGCATCTATGAAAATCAAGATGTCTATGCAAAATTTTCTCTTACTTACAATCCAGATGAGACCCTCTCAACTAGGATCATCAATGGAGGTGGCAAGAACCCTGAATTCAACGAAAACTTGAGGATGAAAATCACTCAACTTGATGCTGTCCTCAAATGTGAGATTTGGATGCTTAGCAGGGCCAGAAACTACATGGAAGACCAGCTTTTGGGATTCACTTTGGTCCCAATTTCACATGTGATTGGCAAAGGAAAGGTAACTCAAGATTATAGTCTGTCTTCCACTGATCTCTTCCATTCCCCAGCTGGAACTGTCAAATTGAGTCTTTCTCTCAACACATCTTTGCCTATTAAACCCTCCACCAGTTCGTTATCCGAATCATCTGCTAACTCATCCATAACTTCAGAAGTTGTGTTGCTTGATCGAAAAGTATCAGAAGTGGTATTAGACCCGGTTGAGTATTCGAGGATCGAATTCCCGGATATCGATGTAGTAAATGAGAGTCAGCAAATGGTGACTGAGTATTTTAATTTGACAAGGCATGGCTGTTCTTGGAGACCAGGTTCCCAAGGACTTGGCTCGTTTCTTCATCTCGGTGCATCTCCTCAGCCTGCTGCTGATCGTGACTATGAAATGACTGTGAGTTCAAATGTGGGAATCCAGGGTGAACCTGTTTCTCCTAATGGGAGTGGGAGCATCCACAACTCTGGCTTCTTAAGTTCCACCACAACAAGCTTGAGTGATGATCGAAACTCCGCTGATTCAATCGAGAAAAAGAATCGTGTGACTGCTGAGTCATCAAATTCTATCAACACTTGTGTCACCACAGGAGCAGCTAATCAAGGTTCTGCTGCTTGTCCAGACACTCCAACGTCAAGGAAGGGAAGAGAAGTTGTAGACACAAAGGATGCAAATTTCTCAGGAAAGGAAGACGAAAGCAGCAAGGAAAAGACGGTTGGTTCTGGTCAATACGGTCAGGTGTTTTCAGCTCCACTCGGGAACATCAACATGGAGGCTGCCGAGCAGTCTGCAATGCAGCAACAAATAGTGGACATGTACATGAGAAGCATGCAACAGTTTACTGAGTCTTTGGCAAAGATGAAGCTCCCAATGGATCTTGATAAAGCAGAATGTGAAGACCGTGGCGAAGTGATTCCTAACCATAACAACAATTTAGAACTTGATAAGAAGAAGGATGGATCGCGGGTGTTCTATGGTAGCCGAGCCTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

458

Amino Acids

50.18

Weight (kDa)

5.08

Isoelectric Point (pI)

40.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12300
fragaria_vesca FvH4_3g27890 FvH4_3g27890 FvH4_3g27890
malus_domestica MD03G1166500.v1.1
prunus_persica Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1
pyrus_communis pycom03g12210
rosa_chinensis RchiOBHm_Chr5g0050621
rosa_laevigata RLG00000034712
rosa_multiflora Rmu_ssc0000350.1_g000030
rosa_roxburghii Rroxscaffold_1G00030180
rosa_rugosa Rorug05G0262100
rosa_samantha Rh5AG334400 Rh5BG343800 Rh5CG371600 Rh5DG356800
rosa_wichuraiana Rw5G031550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 931
AccB7I CCANNNNNTGG 1 cut(s) 346
AccI GTMKAC 1 cut(s) 1035
AccII CGCG 1 cut(s) 1342
AciI CCGC 2 cut(s) 894, 1342
AclWI GGATC 4 cut(s) 221, 614, 1260, 1345
AcsI RAATTY 5 cut(s) 170, 242, 611, 940, 1051
AcuI CTGAAG 1 cut(s) 525
AfaI GTAC 2 cut(s) 35, 1196
AfiI CCNNNNNNNGG 1 cut(s) 346
AflII CTTAAG 1 cut(s) 853
AflIII ACRYGT 2 cut(s) 373, 1191
AgsI TTSAA 5 cut(s) 13, 101, 247, 786, 903
AjnI CCWGG 2 cut(s) 697, 798
AjuI GAANNNNNNNTTGG 2 cut(s) 1077, 1109
AloI GAACNNNNNNTCC 2 cut(s) 685, 717
AluBI AGCT 6 cut(s) 343, 443, 873, 974, 1127, 1243
AluI AGCT 6 cut(s) 343, 443, 873, 974, 1127, 1243
Alw26I GTCTC 2 cut(s) 191, 688
AlwI GGATC 4 cut(s) 221, 614, 1260, 1345
Ama87I CYCGRG 1 cut(s) 1133
AoxI GGCC 1 cut(s) 318
ApeKI GCWGC 6 cut(s) 752, 971, 989, 1077, 1154, 1174
ApoI RAATTY 5 cut(s) 170, 242, 611, 940, 1051
Asp700I GAANNNNTTC 1 cut(s) 1290
AspS9I GGNCC 2 cut(s) 318, 361
AsuC2I CCSGG 2 cut(s) 590, 617
AsuHPI GGTGA 5 cut(s) 71, 83, 664, 815, 952
AvaI CYCGRG 1 cut(s) 1133
AvaII GGWCC 1 cut(s) 361
BbsI GAAGAC 4 cut(s) 342, 408, 1074, 1281
BbvCI CCTCAGC 1 cut(s) 744
BbvI GCAGC 6 cut(s) 739, 976, 983, 1089, 1141, 1186
BccI CCATC 1 cut(s) 1328
BcgI CGANNNNNNTGC 4 cut(s) 503, 537, 1148, 1182
BciT130I CCWGG 2 cut(s) 699, 800
BclI TGATCA 1 cut(s) 79
BcnI CCSGG 2 cut(s) 590, 617
BcoDI GTCTC 2 cut(s) 191, 688
BfaI CTAG 2 cut(s) 120, 210
BfrI CTTAAG 1 cut(s) 853
BisI GCNGC 6 cut(s) 753, 972, 990, 1078, 1155, 1175
BlpI GCTNAGC 1 cut(s) 311
BlsI GCNGC 6 cut(s) 754, 973, 991, 1079, 1156, 1176
Bme1390I CCNGG 4 cut(s) 590, 617, 699, 800
Bme18I GGWCC 1 cut(s) 361
BmeT110I CYCGRG 1 cut(s) 1133
BmgT120I GGNCC 2 cut(s) 318, 361
BmiI GGNNCC 3 cut(s) 29, 363, 703
BmrFI CCNGG 4 cut(s) 590, 617, 699, 800
BmrI ACTGGG 1 cut(s) 33
BmsI GCATC 6 cut(s) 150, 271, 297, 746, 843, 1036
BmuI ACTGGG 1 cut(s) 33
BpiI GAAGAC 4 cut(s) 342, 408, 1074, 1281
BplI GAGNNNNNCTC 4 cut(s) 188, 220, 451, 483
Bpu10I CCTNAGC 1 cut(s) 744
Bpu1102I GCTNAGC 1 cut(s) 311
BpuEI CTTGAG 3 cut(s) 277, 384, 895
BpuMI CCSGG 2 cut(s) 590, 617
Bsa29I ATCGAT 1 cut(s) 624
BsaBI GATNNNNATC 2 cut(s) 78, 266
BsaI GGTCTC 2 cut(s) 191, 688
BsaJI CCNNGG 3 cut(s) 706, 799, 1279
BsaWI WCCGGW 1 cut(s) 30
BsaXI ACNNNNNCTCC 2 cut(s) 685, 715
Bsc4I CCNNNNNNNGG 1 cut(s) 346
Bse1I ACTGG 2 cut(s) 28, 501
Bse3DI GCAATG 1 cut(s) 1176
Bse8I GATNNNNATC 2 cut(s) 78, 266
BseBI CCWGG 2 cut(s) 699, 800
BseCI ATCGAT 1 cut(s) 624
BseDI CCNNGG 3 cut(s) 706, 799, 1279
BseGI GGATG 6 cut(s) 267, 312, 530, 834, 1051, 1339
BseJI GATNNNNATC 2 cut(s) 78, 266
BseLI CCNNNNNNNGG 1 cut(s) 346
BseMI GCAATG 1 cut(s) 1176
BseMII CTCAG 5 cut(s) 648, 758, 921, 1071, 1212
BseNI ACTGG 2 cut(s) 28, 501
BseRI GAGGAG 1 cut(s) 732
BseXI GCAGC 6 cut(s) 739, 976, 983, 1089, 1141, 1186
BseYI CCCAGC 1 cut(s) 439
Bsh1236I CGCG 1 cut(s) 1342
BshFI GGCC 1 cut(s) 320
BshVI ATCGAT 1 cut(s) 624
BsiHKCI CYCGRG 1 cut(s) 1133
BsiSI CCGG 3 cut(s) 31, 590, 617
BslFI GGGAC 1 cut(s) 347
BslI CCNNNNNNNGG 1 cut(s) 346
BsmAI GTCTC 2 cut(s) 191, 688
BsmFI GGGAC 1 cut(s) 347
BsnI GGCC 1 cut(s) 320
Bso31I GGTCTC 2 cut(s) 191, 688
BsoBI CYCGRG 1 cut(s) 1133
Bsp1407I TGTACA 1 cut(s) 1194
Bsp143I GATC 9 cut(s) 79, 213, 424, 559, 606, 757, 883, 1252, 1337
Bsp1720I GCTNAGC 1 cut(s) 311
BspACI CCGC 2 cut(s) 894, 1342
BspANI GGCC 1 cut(s) 320
BspCNI CTCAG 5 cut(s) 649, 757, 922, 1070, 1213
BspDI ATCGAT 1 cut(s) 624
BspFNI CGCG 1 cut(s) 1342
BspLI GGNNCC 3 cut(s) 29, 363, 703
BspPI GGATC 4 cut(s) 221, 614, 1260, 1345
BspTI CTTAAG 1 cut(s) 853
BspTNI GGTCTC 2 cut(s) 191, 688
BsrDI GCAATG 1 cut(s) 1176
BsrGI TGTACA 1 cut(s) 1194
BsrI ACTGG 2 cut(s) 28, 501
BssECI CCNNGG 3 cut(s) 706, 799, 1279
BssMI GATC 9 cut(s) 79, 213, 424, 559, 606, 757, 883, 1252, 1337
BssT1I CCWWGG 1 cut(s) 706
Bst2UI CCWGG 2 cut(s) 699, 800
Bst4CI ACNGT 6 cut(s) 451, 778, 1093, 1112, 1215, 1280
Bst6I CTCTTC 2 cut(s) 434, 1018
BstAFI CTTAAG 1 cut(s) 853
BstAUI TGTACA 1 cut(s) 1194
BstC8I GCNNGC 2 cut(s) 750, 1208
BstDEI CTNAG 6 cut(s) 311, 657, 744, 930, 1057, 1221
BstDSI CCRYGG 1 cut(s) 1279
BstF5I GGATG 6 cut(s) 267, 312, 530, 834, 1051, 1339
BstFNI CGCG 1 cut(s) 1342
BstKTI GATC 9 cut(s) 82, 216, 427, 562, 609, 760, 886, 1255, 1340
BstMAI GTCTC 2 cut(s) 191, 688
BstMBI GATC 9 cut(s) 79, 213, 424, 559, 606, 757, 883, 1252, 1337
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 2 cut(s) 699, 800
BstNSI RCATGY 3 cut(s) 377, 1195, 1210
BstSCI CCNGG 4 cut(s) 588, 615, 697, 798
BstUI CGCG 1 cut(s) 1342
BstV1I GCAGC 6 cut(s) 739, 976, 983, 1089, 1141, 1186
BstV2I GAAGAC 4 cut(s) 342, 408, 1074, 1281
BstX2I RGATCY 1 cut(s) 1252
BstXI CCANNNNNNTGG 2 cut(s) 713, 845
BstYI RGATCY 1 cut(s) 1252
Bsu15I ATCGAT 1 cut(s) 624
BsuRI GGCC 1 cut(s) 320
BsuTUI ATCGAT 1 cut(s) 624
BtgI CCRYGG 1 cut(s) 1279
BtsCI GGATG 6 cut(s) 267, 312, 530, 834, 1051, 1339
BtsIMutI CAGTG 1 cut(s) 420
Cac8I GCNNGC 2 cut(s) 750, 1208
Cfr13I GGNCC 2 cut(s) 318, 361
ClaI ATCGAT 1 cut(s) 624
CsiI ACCWGGT 1 cut(s) 697
Csp6I GTAC 2 cut(s) 34, 1195
CspCI CAANNNNNGTGG 2 cut(s) 208, 243
CviAII CATG 8 cut(s) 116, 331, 374, 680, 1147, 1192, 1198, 1207
CviQI GTAC 2 cut(s) 34, 1195
DdeI CTNAG 6 cut(s) 311, 657, 744, 930, 1057, 1221
DpnI GATC 9 cut(s) 81, 215, 426, 561, 608, 759, 885, 1254, 1339
DpnII GATC 9 cut(s) 79, 213, 424, 559, 606, 757, 883, 1252, 1337
DrdI GACNNNNNNGTC 1 cut(s) 931
DseDI GACNNNNNNGTC 1 cut(s) 931
Eam1104I CTCTTC 2 cut(s) 434, 1018
EarI CTCTTC 2 cut(s) 434, 1018
Eco130I CCWWGG 1 cut(s) 706
Eco31I GGTCTC 2 cut(s) 191, 688
Eco32I GATATC 1 cut(s) 622
Eco47I GGWCC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 525
Eco88I CYCGRG 1 cut(s) 1133
EcoRI GAATTC 2 cut(s) 242, 611
EcoRII CCWGG 2 cut(s) 697, 798
EcoRV GATATC 1 cut(s) 622
EcoT14I CCWWGG 1 cut(s) 706
EcoT22I ATGCAT 1 cut(s) 143
ErhI CCWWGG 1 cut(s) 706
FaeI CATG 8 cut(s) 119, 334, 377, 683, 1150, 1195, 1201, 1210
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FaqI GGGAC 1 cut(s) 347
FatI CATG 8 cut(s) 115, 330, 373, 679, 1146, 1191, 1197, 1206
FauI CCCGC 1 cut(s) 1335
FauNDI CATATG 1 cut(s) 137
FbaI TGATCA 1 cut(s) 79
FblI GTMKAC 1 cut(s) 1035
Fnu4HI GCNGC 6 cut(s) 753, 972, 990, 1078, 1155, 1175
FokI GGATG 6 cut(s) 274, 319, 517, 821, 1058, 1346
Fsp4HI GCNGC 6 cut(s) 753, 972, 990, 1078, 1155, 1175
FspBI CTAG 2 cut(s) 120, 210
GluI GCNGC 6 cut(s) 753, 972, 990, 1078, 1155, 1175
GsaI CCCAGC 1 cut(s) 443
HaeIII GGCC 1 cut(s) 320
HapII CCGG 3 cut(s) 31, 590, 617
Hin1II CATG 8 cut(s) 119, 334, 377, 683, 1150, 1195, 1201, 1210
HindIII AAGCTT 1 cut(s) 871
HpaII CCGG 3 cut(s) 31, 590, 617
HphI GGTGA 5 cut(s) 71, 83, 664, 815, 952
Hpy166II GTNNAC 4 cut(s) 806, 1036, 1189, 1218
Hpy188I TCNGA 7 cut(s) 18, 48, 79, 514, 544, 574, 1373
Hpy8I GTNNAC 4 cut(s) 806, 1036, 1189, 1218
HpyAV CCTTC 2 cut(s) 1012, 1324
HpyCH4III ACNGT 6 cut(s) 451, 778, 1093, 1112, 1215, 1280
HpyCH4IV ACGT 1 cut(s) 1010
HpyCH4V TGCA 7 cut(s) 141, 167, 737, 1049, 1169, 1174, 1210
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 6 cut(s) 311, 657, 744, 930, 1057, 1221
HpySE526I ACGT 1 cut(s) 1010
Hsp92II CATG 8 cut(s) 119, 334, 377, 683, 1150, 1195, 1201, 1210
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 9 cut(s) 79, 213, 424, 559, 606, 757, 883, 1252, 1337
LmnI GCTCC 4 cut(s) 831, 968, 1132, 1248
Lsp1109I GCAGC 6 cut(s) 739, 976, 983, 1089, 1141, 1186
LweI GCATC 6 cut(s) 150, 271, 297, 746, 843, 1036
MabI ACCWGGT 1 cut(s) 697
MaeI CTAG 2 cut(s) 120, 210
MaeII ACGT 1 cut(s) 1010
MaeIII GTNAC 5 cut(s) 394, 652, 761, 922, 958
MalI GATC 9 cut(s) 81, 215, 426, 561, 608, 759, 885, 1254, 1339
MboI GATC 9 cut(s) 79, 213, 424, 559, 606, 757, 883, 1252, 1337
MboII GAAGA 9 cut(s) 347, 408, 421, 716, 1035, 1079, 1286, 1339, 1360
MflI RGATCY 1 cut(s) 1252
MluCI AATT 9 cut(s) 170, 242, 366, 455, 611, 667, 940, 1051, 1309
MlyI GAGTC 4 cut(s) 469, 649, 941, 1232
MmeI TCCRAC 1 cut(s) 1031
MnlI CCTC 8 cut(s) 212, 218, 252, 299, 505, 597, 753, 1144
Mph1103I ATGCAT 1 cut(s) 143
MroXI GAANNNNTTC 1 cut(s) 1290
MseI TTAA 3 cut(s) 489, 666, 854
MslI CAYNNNNRTG 3 cut(s) 732, 1145, 1196
MspA1I CMGCKG 2 cut(s) 443, 896
MspCI CTTAAG 1 cut(s) 853
MspI CCGG 3 cut(s) 31, 590, 617
MspR9I CCNGG 4 cut(s) 590, 617, 699, 800
MvaI CCWGG 2 cut(s) 699, 800
MvnI CGCG 1 cut(s) 1342
MwoI GCNNNNNNNGC 1 cut(s) 116
NciI CCSGG 2 cut(s) 590, 617
NdeI CATATG 1 cut(s) 137
NdeII GATC 9 cut(s) 79, 213, 424, 559, 606, 757, 883, 1252, 1337
NlaIII CATG 8 cut(s) 119, 334, 377, 683, 1150, 1195, 1201, 1210
NlaIV GGNNCC 3 cut(s) 29, 363, 703
NmeAIII GCCGAG 1 cut(s) 1183
NmuCI GTSAC 4 cut(s) 652, 761, 922, 958
NsiI ATGCAT 1 cut(s) 143
NspI RCATGY 3 cut(s) 377, 1195, 1210
PaeI GCATGC 1 cut(s) 1210
PciI ACATGT 2 cut(s) 373, 1191
PdmI GAANNNNTTC 1 cut(s) 1290
PflMI CCANNNNNTGG 1 cut(s) 346
PfoI TCCNGGA 1 cut(s) 615
PkrI GCNGC 6 cut(s) 754, 973, 991, 1079, 1156, 1176
PleI GAGTC 4 cut(s) 468, 648, 940, 1231
PpsI GAGTC 4 cut(s) 468, 648, 940, 1231
PscI ACATGT 2 cut(s) 373, 1191
Psp6I CCWGG 2 cut(s) 697, 798
PspFI CCCAGC 1 cut(s) 439
PspGI CCWGG 2 cut(s) 697, 798
PspN4I GGNNCC 3 cut(s) 29, 363, 703
PspPI GGNCC 2 cut(s) 318, 361
PsuI RGATCY 1 cut(s) 1252
PvuII CAGCTG 1 cut(s) 443
RsaI GTAC 2 cut(s) 35, 1196
RsaNI GTAC 2 cut(s) 34, 1195
RseI CAYNNNNRTG 3 cut(s) 732, 1145, 1196
SaqAI TTAA 3 cut(s) 489, 666, 854
SatI GCNGC 6 cut(s) 753, 972, 990, 1078, 1155, 1175
Sau3AI GATC 9 cut(s) 79, 213, 424, 559, 606, 757, 883, 1252, 1337
Sau96I GGNCC 2 cut(s) 318, 361
SchI GAGTC 4 cut(s) 469, 649, 941, 1232
ScrFI CCNGG 4 cut(s) 590, 617, 699, 800
SexAI ACCWGGT 1 cut(s) 697
SfaNI GCATC 6 cut(s) 150, 271, 297, 746, 843, 1036
SinI GGWCC 1 cut(s) 361
SmiMI CAYNNNNRTG 3 cut(s) 732, 1145, 1196
SmlI CTYRAG 4 cut(s) 256, 399, 853, 874
SmoI CTYRAG 4 cut(s) 256, 399, 853, 874
SphI GCATGC 1 cut(s) 1210
Sse9I AATT 9 cut(s) 170, 242, 366, 455, 611, 667, 940, 1051, 1309
SsiI CCGC 2 cut(s) 894, 1342
SspMI CTAG 2 cut(s) 120, 210
StyD4I CCNGG 4 cut(s) 588, 615, 697, 798
StyI CCWWGG 1 cut(s) 706
TaaI ACNGT 6 cut(s) 451, 778, 1093, 1112, 1215, 1280
TaiI ACGT 1 cut(s) 1013
TaqI TCGA 6 cut(s) 562, 602, 609, 624, 886, 906
TasI AATT 9 cut(s) 170, 242, 366, 455, 611, 667, 940, 1051, 1309
TatI WGTACW 1 cut(s) 1194
Tru1I TTAA 3 cut(s) 489, 666, 854
Tru9I TTAA 3 cut(s) 489, 666, 854
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 4 cut(s) 652, 761, 922, 958
TseI GCWGC 6 cut(s) 752, 971, 989, 1077, 1154, 1174
Tsp45I GTSAC 4 cut(s) 652, 761, 922, 958
TspDTI ATGAA 5 cut(s) 162, 278, 716, 783, 1253
TspRI CASTG 1 cut(s) 427
Van91I CCANNNNNTGG 1 cut(s) 346
Vha464I CTTAAG 1 cut(s) 853
VpaK11BI GGWCC 1 cut(s) 361
XapI RAATTY 5 cut(s) 170, 242, 611, 940, 1051
XceI RCATGY 3 cut(s) 377, 1195, 1210
XcmI CCANNNNNNNNNTGG 2 cut(s) 328, 440
XmiI GTMKAC 1 cut(s) 1035
XmnI GAANNNNTTC 1 cut(s) 1290
XspI CTAG 2 cut(s) 120, 210
Zsp2I ATGCAT 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.