Rw5G031550

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0012150
Physical Location & Seq
Reverse (-)
52198036 .. 52199424
1389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G031550.1

Sequence Viewer

Length: 1266 bp
ATGGAATCATTCAGTCAAACAACTGGATTCCGGTACAACCTGAATTCAAACACAACAGGCGCCGATGATGCCGAGTTTTCAGGGATTCTTGAGATCTATGCTCATCATGCTAGGAACCTTCACAACATATGCATCTATGAAAACCAAGATGTCTATGCAAAATTCTCTCTTACTTACAATCCAGATGAGACCCTCTCAACTAGGATCATCAATGGAGGTGGCAAGAATCCAGAATTCAATGAAAACTTGAGGATGAAAATCGCTCAAATTGATGCTGTCTTACTTACAATCCAGATGAGACCCTCTCAACTAGGATCATCAATGGAGGGCCAGAACTATATGGAAGACCAGCTCTTGGGATTTACTTTGGTCCCACTTTCTCAAGTGATTGGGAAAGGAAAGCTGACTCGAGATTATAGTCTTTCTTCCACTGACCTCTTCCATTCCCCAGCTGGAACTGTCCAATTGACTCTTTCTCTCATCCCATCTTTGCCTATCAAATTGTCTGCTAACTCATCCATAACTTCAGAAGTAGTATTGCTTGATCGAAAGAATCCAGAAGAAGTAATGTTAGACCCAGTTGAGTATTCGAGGATCGAATTCCCTGATATCAATGCAGTGAGTCAGAATCAGCAAATGGTGACTCAGTACTTTGACTTGGCAAAGCACAAGCATGGTTCTCAAGGACTAGCTGCATCATTTCTTCGTCTTGGTGCATCTCCTGATCAGGACTATGAAATGATTGTGAGCTCATCGCCGCCTAATGAGAGCATCCACAACTCTGGCTTCTTAAGTTCCACCACAACAAGCTTGAGTGATGACCGGAACAACAATTCTGCTGATTCAATCGAGAAAAAGAGTCGTGTGGCTTCTGTCACCAGTGGAGTAGCTCATCAAAATTTTGCTGCTTGTCCAGACACTCCAACCTCAAGAAAGGGAATAGAAGCCTCTTCCGGAGATGAAAAGTCGAGAGATGAAGAAGGATCAAAAGTTGGTTCAGGTCAATTTGGTCGGGTGTATTCTGCTCCACATGGGAATATGAACTCAGAGGCTGAGCAATCTGCAATGCAGCAACAAATAGTCGACATGTACATGAGAAGTATGCAACAGTTTACCGAGTCTCTGGCAAACATGAAGCTCCCGATGGATCTTGATAAACCTGAATGTGAAGACCGTGGTGAAGTGATTCCGAACAACAACAACTTAGAACTTGATAAAAAGAAGGATGGATCAAGAGTCTTCTATGGTAGCAGGGCCTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

421

Amino Acids

46.5

Weight (kDa)

5.0

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12300
fragaria_vesca FvH4_3g27890 FvH4_3g27890 FvH4_3g27890
malus_domestica MD03G1166500.v1.1
prunus_persica Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1
pyrus_communis pycom03g12210
rosa_chinensis RchiOBHm_Chr5g0050621
rosa_laevigata RLG00000034712
rosa_multiflora Rmu_ssc0000350.1_g000030
rosa_roxburghii Rroxscaffold_1G00030180
rosa_rugosa Rorug05G0262100
rosa_samantha Rh5AG334400 Rh5BG343800 Rh5CG371600 Rh5DG356800
rosa_wichuraiana Rw5G031550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 59
AccB7I CCANNNNNTGG 1 cut(s) 355
AccI GTMKAC 1 cut(s) 1083
AccIII TCCGGA 1 cut(s) 953
AciI CCGC 1 cut(s) 758
AclWI GGATC 6 cut(s) 212, 322, 602, 991, 1155, 1237
AcsI RAATTY 5 cut(s) 43, 161, 233, 599, 898
AcuI CTGAAG 1 cut(s) 510
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 3 cut(s) 35, 650, 1091
AfiI CCNNNNNNNGG 1 cut(s) 355
AflII CTTAAG 1 cut(s) 790
AflIII ACRYGT 1 cut(s) 1086
AgsI TTSAA 3 cut(s) 48, 238, 846
AluBI AGCT 8 cut(s) 352, 403, 452, 692, 750, 810, 890, 1138
AluI AGCT 8 cut(s) 352, 403, 452, 692, 750, 810, 890, 1138
Alw21I GWGCWC 1 cut(s) 752
Alw26I GTCTC 3 cut(s) 182, 292, 1125
AlwI GGATC 6 cut(s) 212, 322, 602, 991, 1155, 1237
AlwNI CAGNNNCTG 1 cut(s) 1052
Ama87I CYCGRG 1 cut(s) 408
Aor13HI TCCGGA 1 cut(s) 953
AoxI GGCC 2 cut(s) 328, 1254
ApeKI GCWGC 3 cut(s) 692, 905, 1069
ApoI RAATTY 5 cut(s) 43, 161, 233, 599, 898
Asp700I GAANNNNTTC 1 cut(s) 1185
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 3 cut(s) 328, 370, 1254
AsuHPI GGTGA 3 cut(s) 652, 868, 1190
AvaI CYCGRG 1 cut(s) 408
AvaII GGWCC 1 cut(s) 370
BanI GGYRCC 1 cut(s) 59
BanII GRGCYC 1 cut(s) 752
BbsI GAAGAC 3 cut(s) 351, 1176, 1231
Bbv12I GWGCWC 1 cut(s) 752
BbvI GCAGC 3 cut(s) 679, 892, 1081
BccI CCATC 3 cut(s) 493, 1138, 1220
BclI TGATCA 1 cut(s) 724
BcoDI GTCTC 3 cut(s) 182, 292, 1125
BfaI CTAG 4 cut(s) 111, 201, 311, 689
BfoI RGCGCY 1 cut(s) 63
BfrI CTTAAG 1 cut(s) 790
BglII AGATCT 1 cut(s) 93
BisI GCNGC 4 cut(s) 693, 758, 906, 1070
BlpI GCTNAGC 1 cut(s) 1053
BlsI GCNGC 4 cut(s) 694, 759, 907, 1071
BmcAI AGTACT 1 cut(s) 650
Bme18I GGWCC 1 cut(s) 370
BmeT110I CYCGRG 1 cut(s) 408
BmgT120I GGNCC 3 cut(s) 328, 370, 1254
BmiI GGNNCC 3 cut(s) 61, 116, 372
BmrI ACTGGG 1 cut(s) 572
BmsI GCATC 6 cut(s) 58, 141, 262, 704, 725, 780
BmuI ACTGGG 1 cut(s) 572
BpiI GAAGAC 3 cut(s) 351, 1176, 1231
BplI GAGNNNNNCTC 4 cut(s) 179, 211, 289, 321
Bpu1102I GCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 6 cut(s) 110, 268, 366, 666, 832, 913
BsaBI GATNNNNATC 1 cut(s) 257
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 2 cut(s) 182, 292
BsaJI CCNNGG 1 cut(s) 1174
BsaWI WCCGGW 3 cut(s) 30, 822, 953
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse1I ACTGG 3 cut(s) 28, 578, 879
Bse3DI GCAATG 1 cut(s) 1071
Bse8I GATNNNNATC 1 cut(s) 257
BseAI TCCGGA 1 cut(s) 953
BseDI CCNNGG 1 cut(s) 1174
BseGI GGATG 5 cut(s) 258, 480, 515, 771, 1231
BseJI GATNNNNATC 1 cut(s) 257
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMI GCAATG 1 cut(s) 1071
BseMII CTCAG 3 cut(s) 659, 1044, 1059
BseNI ACTGG 3 cut(s) 28, 578, 879
BseXI GCAGC 3 cut(s) 679, 892, 1081
BseYI CCCAGC 1 cut(s) 448
BshFI GGCC 2 cut(s) 330, 1256
BshNI GGYRCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 752
BsiHKCI CYCGRG 1 cut(s) 408
BsiSI CCGG 3 cut(s) 31, 823, 954
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 3 cut(s) 182, 292, 1125
BsmFI GGGAC 1 cut(s) 356
BsnI GGCC 2 cut(s) 330, 1256
Bso31I GGTCTC 2 cut(s) 182, 292
BsoBI CYCGRG 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 752
Bsp13I TCCGGA 1 cut(s) 953
Bsp1407I TGTACA 1 cut(s) 1089
Bsp143I GATC 9 cut(s) 93, 204, 314, 544, 594, 724, 983, 1147, 1229
Bsp1720I GCTNAGC 1 cut(s) 1053
BspACI CCGC 1 cut(s) 758
BspANI GGCC 2 cut(s) 330, 1256
BspCNI CTCAG 3 cut(s) 658, 1045, 1058
BspEI TCCGGA 1 cut(s) 953
BspLI GGNNCC 3 cut(s) 61, 116, 372
BspPI GGATC 6 cut(s) 212, 322, 602, 991, 1155, 1237
BspT107I GGYRCC 1 cut(s) 59
BspTI CTTAAG 1 cut(s) 790
BspTNI GGTCTC 2 cut(s) 182, 292
BsrDI GCAATG 1 cut(s) 1071
BsrGI TGTACA 1 cut(s) 1089
BsrI ACTGG 3 cut(s) 28, 578, 879
BssECI CCNNGG 1 cut(s) 1174
BssMI GATC 9 cut(s) 93, 204, 314, 544, 594, 724, 983, 1147, 1229
BssNI GRCGYC 1 cut(s) 60
Bst4CI ACNGT 3 cut(s) 460, 1110, 1175
Bst6I CTCTTC 2 cut(s) 443, 955
BstACI GRCGYC 1 cut(s) 60
BstAFI CTTAAG 1 cut(s) 790
BstAUI TGTACA 1 cut(s) 1089
BstDEI CTNAG 4 cut(s) 645, 1045, 1053, 1204
BstDSI CCRYGG 1 cut(s) 1174
BstF5I GGATG 5 cut(s) 258, 480, 515, 771, 1231
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 9 cut(s) 96, 207, 317, 547, 597, 727, 986, 1150, 1232
BstMAI GTCTC 3 cut(s) 182, 292, 1125
BstMBI GATC 9 cut(s) 93, 204, 314, 544, 594, 724, 983, 1147, 1229
BstMWI GCNNNNNNNGC 2 cut(s) 68, 107
BstNSI RCATGY 1 cut(s) 1090
BstV1I GCAGC 3 cut(s) 679, 892, 1081
BstV2I GAAGAC 3 cut(s) 351, 1176, 1231
BstX2I RGATCY 2 cut(s) 93, 1147
BstXI CCANNNNNNTGG 1 cut(s) 782
BstYI RGATCY 2 cut(s) 93, 1147
BsuRI GGCC 2 cut(s) 330, 1256
BtgI CCRYGG 1 cut(s) 1174
BtgZI GCGATG 1 cut(s) 738
BtsCI GGATG 5 cut(s) 258, 480, 515, 771, 1231
BtsI GCAGTG 1 cut(s) 624
BtsIMutI CAGTG 3 cut(s) 429, 624, 886
CaiI CAGNNNCTG 1 cut(s) 1052
CfoI GCGC 1 cut(s) 62
Cfr13I GGNCC 3 cut(s) 328, 370, 1254
Csp6I GTAC 3 cut(s) 34, 649, 1090
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviAII CATG 6 cut(s) 107, 674, 1031, 1087, 1093, 1132
CviQI GTAC 3 cut(s) 34, 649, 1090
DdeI CTNAG 4 cut(s) 645, 1045, 1053, 1204
DinI GGCGCC 1 cut(s) 61
DpnI GATC 9 cut(s) 95, 206, 316, 546, 596, 726, 985, 1149, 1231
DpnII GATC 9 cut(s) 93, 204, 314, 544, 594, 724, 983, 1147, 1229
Eam1104I CTCTTC 2 cut(s) 443, 955
EarI CTCTTC 2 cut(s) 443, 955
Ecl136II GAGCTC 1 cut(s) 750
Eco24I GRGCYC 1 cut(s) 752
Eco31I GGTCTC 2 cut(s) 182, 292
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 750
Eco57I CTGAAG 1 cut(s) 510
Eco88I CYCGRG 1 cut(s) 408
EcoICRI GAGCTC 1 cut(s) 750
EcoO109I RGGNCCY 1 cut(s) 1254
EcoRI GAATTC 3 cut(s) 43, 233, 599
EcoRV GATATC 1 cut(s) 610
EcoT22I ATGCAT 1 cut(s) 134
EcoT38I GRGCYC 1 cut(s) 752
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
FaeI CATG 6 cut(s) 110, 677, 1034, 1090, 1096, 1135
FaqI GGGAC 1 cut(s) 356
FatI CATG 6 cut(s) 106, 673, 1030, 1086, 1092, 1131
FauNDI CATATG 1 cut(s) 128
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 1 cut(s) 1083
Fnu4HI GCNGC 4 cut(s) 693, 758, 906, 1070
FokI GGATG 5 cut(s) 265, 467, 502, 758, 1238
FriOI GRGCYC 1 cut(s) 752
Fsp4HI GCNGC 4 cut(s) 693, 758, 906, 1070
FspBI CTAG 4 cut(s) 111, 201, 311, 689
GlaI GCGC 1 cut(s) 61
GluI GCNGC 4 cut(s) 693, 758, 906, 1070
GsaI CCCAGC 1 cut(s) 452
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 2 cut(s) 330, 1256
HapII CCGG 3 cut(s) 31, 823, 954
HhaI GCGC 1 cut(s) 62
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 6 cut(s) 110, 677, 1034, 1090, 1096, 1135
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HincII GTYRAC 1 cut(s) 1084
HindII GTYRAC 1 cut(s) 1084
HindIII AAGCTT 1 cut(s) 808
HpaII CCGG 3 cut(s) 31, 823, 954
HphI GGTGA 3 cut(s) 652, 868, 1190
Hpy166II GTNNAC 2 cut(s) 1084, 1113
Hpy188I TCNGA 5 cut(s) 529, 627, 1048, 1191, 1265
Hpy8I GTNNAC 2 cut(s) 1084, 1113
HpyAV CCTTC 3 cut(s) 128, 974, 1216
HpyCH4III ACNGT 3 cut(s) 460, 1110, 1175
HpyCH4V TGCA 8 cut(s) 132, 158, 617, 695, 716, 1064, 1069, 1105
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 107
HpyF3I CTNAG 4 cut(s) 645, 1045, 1053, 1204
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 6 cut(s) 110, 677, 1034, 1090, 1096, 1135
HspAI GCGC 1 cut(s) 60
KasI GGCGCC 1 cut(s) 59
Kpn2I TCCGGA 1 cut(s) 953
Ksp22I TGATCA 1 cut(s) 724
Kzo9I GATC 9 cut(s) 93, 204, 314, 544, 594, 724, 983, 1147, 1229
LmnI GCTCC 2 cut(s) 1030, 1143
Lsp1109I GCAGC 3 cut(s) 679, 892, 1081
LweI GCATC 6 cut(s) 58, 141, 262, 704, 725, 780
MaeI CTAG 4 cut(s) 111, 201, 311, 689
MaeIII GTNAC 2 cut(s) 640, 874
MalI GATC 9 cut(s) 95, 206, 316, 546, 596, 726, 985, 1149, 1231
MboI GATC 9 cut(s) 93, 204, 314, 544, 594, 724, 983, 1147, 1229
MfeI CAATTG 1 cut(s) 464
MflI RGATCY 2 cut(s) 93, 1147
MhlI GDGCHC 1 cut(s) 752
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 7 cut(s) 400, 463, 631, 637, 868, 1127, 1245
MmeI TCCRAC 1 cut(s) 947
Mph1103I ATGCAT 1 cut(s) 134
MroI TCCGGA 1 cut(s) 953
MroXI GAANNNNTTC 1 cut(s) 1185
MseI TTAA 1 cut(s) 791
MslI CAYNNNNRTG 2 cut(s) 672, 1091
MspA1I CMGCKG 1 cut(s) 452
MspCI CTTAAG 1 cut(s) 790
MspI CCGG 3 cut(s) 31, 823, 954
MunI CAATTG 1 cut(s) 464
MwoI GCNNNNNNNGC 2 cut(s) 68, 107
NarI GGCGCC 1 cut(s) 60
NdeI CATATG 1 cut(s) 128
NdeII GATC 9 cut(s) 93, 204, 314, 544, 594, 724, 983, 1147, 1229
NlaIII CATG 6 cut(s) 110, 677, 1034, 1090, 1096, 1135
NlaIV GGNNCC 3 cut(s) 61, 116, 372
NmeAIII GCCGAG 1 cut(s) 97
NmuCI GTSAC 2 cut(s) 640, 874
NsiI ATGCAT 1 cut(s) 134
NspI RCATGY 1 cut(s) 1090
PaeR7I CTCGAG 1 cut(s) 408
PciI ACATGT 1 cut(s) 1086
PdmI GAANNNNTTC 1 cut(s) 1185
PfeI GAWTC 8 cut(s) 5, 27, 85, 226, 553, 628, 842, 1186
PflMI CCANNNNNTGG 1 cut(s) 355
PkrI GCNGC 4 cut(s) 694, 759, 907, 1071
PleI GAGTC 7 cut(s) 400, 463, 630, 637, 867, 1126, 1244
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 7 cut(s) 400, 463, 630, 637, 867, 1126, 1244
PscI ACATGT 1 cut(s) 1086
Psp124BI GAGCTC 1 cut(s) 752
PspFI CCCAGC 1 cut(s) 448
PspN4I GGNNCC 3 cut(s) 61, 116, 372
PspPI GGNCC 3 cut(s) 328, 370, 1254
PstNI CAGNNNCTG 1 cut(s) 1052
PsuI RGATCY 2 cut(s) 93, 1147
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 3 cut(s) 35, 650, 1091
RsaNI GTAC 3 cut(s) 34, 649, 1090
RseI CAYNNNNRTG 2 cut(s) 672, 1091
SacI GAGCTC 1 cut(s) 752
SalI GTCGAC 1 cut(s) 1082
SaqAI TTAA 1 cut(s) 791
SatI GCNGC 4 cut(s) 693, 758, 906, 1070
Sau3AI GATC 9 cut(s) 93, 204, 314, 544, 594, 724, 983, 1147, 1229
Sau96I GGNCC 3 cut(s) 328, 370, 1254
ScaI AGTACT 1 cut(s) 650
SchI GAGTC 7 cut(s) 400, 463, 631, 637, 868, 1127, 1245
SduI GDGCHC 1 cut(s) 752
SfaNI GCATC 6 cut(s) 58, 141, 262, 704, 725, 780
SfoI GGCGCC 1 cut(s) 61
Sfr274I CTCGAG 1 cut(s) 408
SinI GGWCC 1 cut(s) 370
SlaI CTCGAG 1 cut(s) 408
SmiMI CAYNNNNRTG 2 cut(s) 672, 1091
SmlI CTYRAG 8 cut(s) 89, 247, 381, 408, 681, 790, 811, 928
SmoI CTYRAG 8 cut(s) 89, 247, 381, 408, 681, 790, 811, 928
SsiI CCGC 1 cut(s) 758
SspDI GGCGCC 1 cut(s) 59
SspMI CTAG 4 cut(s) 111, 201, 311, 689
SstI GAGCTC 1 cut(s) 752
TaaI ACNGT 3 cut(s) 460, 1110, 1175
TaqI TCGA 7 cut(s) 409, 547, 590, 597, 849, 968, 1083
TatI WGTACW 2 cut(s) 648, 1089
TauI GCSGC 1 cut(s) 760
TfiI GAWTC 8 cut(s) 5, 27, 85, 226, 553, 628, 842, 1186
Tru1I TTAA 1 cut(s) 791
Tru9I TTAA 1 cut(s) 791
TscAI CASTG 3 cut(s) 436, 624, 886
TseFI GTSAC 2 cut(s) 640, 874
TseI GCWGC 3 cut(s) 692, 905, 1069
Tsp45I GTSAC 2 cut(s) 640, 874
TspDTI ATGAA 8 cut(s) 153, 255, 269, 750, 975, 990, 1055, 1148
TspRI CASTG 3 cut(s) 436, 624, 886
Van91I CCANNNNNTGG 1 cut(s) 355
Vha464I CTTAAG 1 cut(s) 790
VpaK11BI GGWCC 1 cut(s) 370
XapI RAATTY 5 cut(s) 43, 161, 233, 599, 898
XceI RCATGY 1 cut(s) 1090
XcmI CCANNNNNNNNNTGG 1 cut(s) 449
XhoI CTCGAG 1 cut(s) 408
XmiI GTMKAC 1 cut(s) 1083
XmnI GAANNNNTTC 1 cut(s) 1185
XspI CTAG 4 cut(s) 111, 201, 311, 689
ZrmI AGTACT 1 cut(s) 650
Zsp2I ATGCAT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.