Rh5CG371600

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0012150
Physical Location & Seq
Reverse (-)
47625128 .. 47626861
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG371600.1

Sequence Viewer

Length: 1287 bp
ATGGAATCATTCAGTCAAACAACTGGATTCCGGTACAACCTGAATTCAAACACAACAGGCGGCGATGATGATGCCGAGTTTTCAGGGATTCTTGAGATCTATGCTCATCATGCTAGGAACCTTCACAACATATGCATCTATGAAAACCAAGATGTCTACGCAAAATTCTCTCTTACTTACAATCCAGATGAGACCCTCTCAACTAGGATCATCAATGGAGGTGGCAAGAATCCAGAATTCAATGAAAACTTGAGGATGAAAATCACTCAAATTGATGCTGTTCTGAAATGTGAGATTTGGATGCTTAGCAGGGCCAGAAACTATATGGAAGACCAGCTCTTGGGATTTACTTTGGTCCCAATTTCGCAAGTGATTGGGAAAGGAAAGCTGACTCGAGATTATAGTCTTTCTTCCACTGACCTCTTCCATTCCCCAGCTGGAACTGTCCAATTGACTCTTTCTCTCATCCCATCTTTGCCTCCTATCAAATTGTCTGCTAACTCATCCATAACTTCAGAAGTAGTATTGCTTGATCGAAAGATTAATTCAGAAGAAGTAATGTTAGACCCAGTTGAGTATTCGAGGATCGAATTCCCTGATATCAATGCAGTGAGTCAGAATCAGCAAATGGTGACTCAGTACTTTGACTTGGCAAAGCACAAGCATGGTTCTCAAGGACTAGCTGCATCATTTCTTCGTCTTGGTGCATCTCCTGATCAGGACTATGAAATGATTGTGAGCTCATCGCCTAATGAGAGCATCCACAACTCTGGCTTCTTAAGTTCCACCACAACAAGCTTGAGTGATGACCGAAACAACAATTCTGCTGATTCAATCGAGAAAAAGAGTCGTGTGGCTTCTGTCACCAGTGGAGTAGCTCATCAAAATTTTGCTGCTTGTCCAGACACTCCAACCTCAAGAAAGGGACTAGAAGCCTCTTCCGGAGATGAAAAGTCGAGTTTCTTGGGCAAGGAAGATGAAGAGGAAGAGGAGATGAAGAAGGATCAAAAAGTTGGTTCAGGTCAATTTGGTCAGGTGTATTCTGCTCCACATGGGAATATGAACTCAGAGGCTGAGCAATCTGCAATGCAGCAACAAATAGTCGACATGTACATGAGAAGTATGCAACAGTTTACTGAGTCTCTGGCAAACATGAAGCTCCCGATGGATCTTGATAAACCTGAATGTGAAGACCGTGGTGAAGTGATTCCGAACAACAACAACTTAGAACTTGATAAAAAGAAGGAAGGATCAAGAGTCTTCTATGGTAGCAGGGCCTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

428

Amino Acids

47.63

Weight (kDa)

4.91

Isoelectric Point (pI)

42.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 29 - 133 1.3e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12300
fragaria_vesca FvH4_3g27890 FvH4_3g27890 FvH4_3g27890
malus_domestica MD03G1166500.v1.1
prunus_persica Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1
pyrus_communis pycom03g12210
rosa_chinensis RchiOBHm_Chr5g0050621
rosa_laevigata RLG00000034712
rosa_multiflora Rmu_ssc0000350.1_g000030
rosa_roxburghii Rroxscaffold_1G00030180
rosa_rugosa Rorug05G0262100
rosa_samantha Rh5AG334400 Rh5BG343800 Rh5CG371600 Rh5DG356800
rosa_wichuraiana Rw5G031550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 340
AccI GTMKAC 2 cut(s) 156, 1104
AccIII TCCGGA 1 cut(s) 941
AciI CCGC 1 cut(s) 60
AclWI GGATC 5 cut(s) 215, 593, 1011, 1176, 1258
AcsI RAATTY 5 cut(s) 43, 164, 236, 590, 886
AcuI CTGAAG 1 cut(s) 498
AfaI GTAC 3 cut(s) 35, 641, 1112
AfiI CCNNNNNNNGG 1 cut(s) 340
AflII CTTAAG 1 cut(s) 778
AflIII ACRYGT 1 cut(s) 1107
AgsI TTSAA 3 cut(s) 48, 241, 834
AluBI AGCT 8 cut(s) 337, 388, 437, 683, 741, 798, 878, 1159
AluI AGCT 8 cut(s) 337, 388, 437, 683, 741, 798, 878, 1159
Alw21I GWGCWC 1 cut(s) 743
Alw26I GTCTC 2 cut(s) 185, 1146
AlwI GGATC 5 cut(s) 215, 593, 1011, 1176, 1258
AlwNI CAGNNNCTG 1 cut(s) 1073
Ama87I CYCGRG 1 cut(s) 393
Aor13HI TCCGGA 1 cut(s) 941
AoxI GGCC 2 cut(s) 312, 1275
ApeKI GCWGC 3 cut(s) 683, 893, 1090
ApoI RAATTY 5 cut(s) 43, 164, 236, 590, 886
AseI ATTAAT 1 cut(s) 543
Asp700I GAANNNNTTC 1 cut(s) 1206
AspS9I GGNCC 3 cut(s) 312, 355, 1275
AsuHPI GGTGA 3 cut(s) 643, 856, 1211
AvaI CYCGRG 1 cut(s) 393
AvaII GGWCC 1 cut(s) 355
BanII GRGCYC 1 cut(s) 743
BbsI GAAGAC 3 cut(s) 336, 1197, 1252
Bbv12I GWGCWC 1 cut(s) 743
BbvI GCAGC 3 cut(s) 670, 880, 1102
BccI CCATC 2 cut(s) 478, 1159
BcgI CGANNNNNNTGC 2 cut(s) 53, 87
BclI TGATCA 1 cut(s) 715
BcoDI GTCTC 2 cut(s) 185, 1146
BfaI CTAG 4 cut(s) 114, 204, 680, 929
BfrI CTTAAG 1 cut(s) 778
BglII AGATCT 1 cut(s) 96
BisI GCNGC 4 cut(s) 61, 684, 894, 1091
BlpI GCTNAGC 2 cut(s) 305, 1074
BlsI GCNGC 4 cut(s) 62, 685, 895, 1092
BmcAI AGTACT 1 cut(s) 641
Bme18I GGWCC 1 cut(s) 355
BmeT110I CYCGRG 1 cut(s) 393
BmgT120I GGNCC 3 cut(s) 312, 355, 1275
BmiI GGNNCC 2 cut(s) 119, 357
BmrI ACTGGG 1 cut(s) 563
BmsI GCATC 7 cut(s) 61, 144, 265, 291, 695, 716, 768
BmuI ACTGGG 1 cut(s) 563
BpiI GAAGAC 3 cut(s) 336, 1197, 1252
BplI GAGNNNNNCTC 2 cut(s) 182, 214
Bpu1102I GCTNAGC 2 cut(s) 305, 1074
BpuEI CTTGAG 5 cut(s) 113, 271, 657, 820, 901
BsaBI GATNNNNATC 1 cut(s) 260
BsaI GGTCTC 1 cut(s) 185
BsaJI CCNNGG 1 cut(s) 1195
BsaWI WCCGGW 2 cut(s) 30, 941
Bsc4I CCNNNNNNNGG 1 cut(s) 340
Bse1I ACTGG 3 cut(s) 28, 569, 867
Bse3DI GCAATG 1 cut(s) 1092
Bse8I GATNNNNATC 1 cut(s) 260
BseAI TCCGGA 1 cut(s) 941
BseDI CCNNGG 1 cut(s) 1195
BseGI GGATG 5 cut(s) 261, 306, 465, 503, 759
BseJI GATNNNNATC 1 cut(s) 260
BseLI CCNNNNNNNGG 1 cut(s) 340
BseMI GCAATG 1 cut(s) 1092
BseMII CTCAG 4 cut(s) 650, 1065, 1080, 1128
BseNI ACTGG 3 cut(s) 28, 569, 867
BseRI GAGGAG 1 cut(s) 1004
BseXI GCAGC 3 cut(s) 670, 880, 1102
BseYI CCCAGC 1 cut(s) 433
BshFI GGCC 2 cut(s) 314, 1277
BsiHKAI GWGCWC 1 cut(s) 743
BsiHKCI CYCGRG 1 cut(s) 393
BsiSI CCGG 2 cut(s) 31, 942
BslFI GGGAC 2 cut(s) 341, 939
BslI CCNNNNNNNGG 1 cut(s) 340
BsmAI GTCTC 2 cut(s) 185, 1146
BsmFI GGGAC 2 cut(s) 341, 939
BsnI GGCC 2 cut(s) 314, 1277
Bso31I GGTCTC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 393
Bsp1286I GDGCHC 1 cut(s) 743
Bsp13I TCCGGA 1 cut(s) 941
Bsp1407I TGTACA 1 cut(s) 1110
Bsp143I GATC 8 cut(s) 96, 207, 532, 585, 715, 1003, 1168, 1250
Bsp1720I GCTNAGC 2 cut(s) 305, 1074
BspACI CCGC 1 cut(s) 60
BspANI GGCC 2 cut(s) 314, 1277
BspCNI CTCAG 4 cut(s) 649, 1066, 1079, 1129
BspEI TCCGGA 1 cut(s) 941
BspLI GGNNCC 2 cut(s) 119, 357
BspPI GGATC 5 cut(s) 215, 593, 1011, 1176, 1258
BspTI CTTAAG 1 cut(s) 778
BspTNI GGTCTC 1 cut(s) 185
BsrDI GCAATG 1 cut(s) 1092
BsrGI TGTACA 1 cut(s) 1110
BsrI ACTGG 3 cut(s) 28, 569, 867
BssECI CCNNGG 1 cut(s) 1195
BssMI GATC 8 cut(s) 96, 207, 532, 585, 715, 1003, 1168, 1250
Bst4CI ACNGT 3 cut(s) 445, 1131, 1196
Bst6I CTCTTC 4 cut(s) 428, 943, 975, 981
BstAFI CTTAAG 1 cut(s) 778
BstAUI TGTACA 1 cut(s) 1110
BstDEI CTNAG 6 cut(s) 305, 636, 1066, 1074, 1137, 1225
BstDSI CCRYGG 1 cut(s) 1195
BstF5I GGATG 5 cut(s) 261, 306, 465, 503, 759
BstKTI GATC 8 cut(s) 99, 210, 535, 588, 718, 1006, 1171, 1253
BstMAI GTCTC 2 cut(s) 185, 1146
BstMBI GATC 8 cut(s) 96, 207, 532, 585, 715, 1003, 1168, 1250
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstNSI RCATGY 1 cut(s) 1111
BstV1I GCAGC 3 cut(s) 670, 880, 1102
BstV2I GAAGAC 3 cut(s) 336, 1197, 1252
BstX2I RGATCY 2 cut(s) 96, 1168
BstXI CCANNNNNNTGG 1 cut(s) 770
BstYI RGATCY 2 cut(s) 96, 1168
BsuRI GGCC 2 cut(s) 314, 1277
BtgI CCRYGG 1 cut(s) 1195
BtgZI GCGATG 2 cut(s) 78, 729
BtsCI GGATG 5 cut(s) 261, 306, 465, 503, 759
BtsI GCAGTG 1 cut(s) 615
BtsIMutI CAGTG 3 cut(s) 414, 615, 874
CaiI CAGNNNCTG 1 cut(s) 1073
Cfr13I GGNCC 3 cut(s) 312, 355, 1275
Csp6I GTAC 3 cut(s) 34, 640, 1111
CspCI CAANNNNNGTGG 2 cut(s) 202, 237
CviAII CATG 6 cut(s) 110, 665, 1052, 1108, 1114, 1153
CviQI GTAC 3 cut(s) 34, 640, 1111
DdeI CTNAG 6 cut(s) 305, 636, 1066, 1074, 1137, 1225
DpnI GATC 8 cut(s) 98, 209, 534, 587, 717, 1005, 1170, 1252
DpnII GATC 8 cut(s) 96, 207, 532, 585, 715, 1003, 1168, 1250
Eam1104I CTCTTC 4 cut(s) 428, 943, 975, 981
EarI CTCTTC 4 cut(s) 428, 943, 975, 981
Ecl136II GAGCTC 1 cut(s) 741
Eco24I GRGCYC 1 cut(s) 743
Eco31I GGTCTC 1 cut(s) 185
Eco32I GATATC 1 cut(s) 601
Eco47I GGWCC 1 cut(s) 355
Eco53kI GAGCTC 1 cut(s) 741
Eco57I CTGAAG 1 cut(s) 498
Eco88I CYCGRG 1 cut(s) 393
EcoICRI GAGCTC 1 cut(s) 741
EcoO109I RGGNCCY 1 cut(s) 1275
EcoRI GAATTC 3 cut(s) 43, 236, 590
EcoRV GATATC 1 cut(s) 601
EcoT22I ATGCAT 1 cut(s) 137
EcoT38I GRGCYC 1 cut(s) 743
FaeI CATG 6 cut(s) 113, 668, 1055, 1111, 1117, 1156
FaqI GGGAC 2 cut(s) 341, 939
FatI CATG 6 cut(s) 109, 664, 1051, 1107, 1113, 1152
FauNDI CATATG 1 cut(s) 131
FbaI TGATCA 1 cut(s) 715
FblI GTMKAC 2 cut(s) 156, 1104
Fnu4HI GCNGC 4 cut(s) 61, 684, 894, 1091
FokI GGATG 5 cut(s) 268, 313, 452, 490, 746
FriOI GRGCYC 1 cut(s) 743
Fsp4HI GCNGC 4 cut(s) 61, 684, 894, 1091
FspBI CTAG 4 cut(s) 114, 204, 680, 929
GluI GCNGC 4 cut(s) 61, 684, 894, 1091
GsaI CCCAGC 1 cut(s) 437
HaeIII GGCC 2 cut(s) 314, 1277
HapII CCGG 2 cut(s) 31, 942
Hin1II CATG 6 cut(s) 113, 668, 1055, 1111, 1117, 1156
HincII GTYRAC 1 cut(s) 1105
HindII GTYRAC 1 cut(s) 1105
HindIII AAGCTT 1 cut(s) 796
HpaII CCGG 2 cut(s) 31, 942
HphI GGTGA 3 cut(s) 643, 856, 1211
Hpy166II GTNNAC 3 cut(s) 157, 1105, 1134
Hpy188I TCNGA 7 cut(s) 285, 517, 550, 618, 1069, 1212, 1286
Hpy8I GTNNAC 3 cut(s) 157, 1105, 1134
HpyAV CCTTC 4 cut(s) 131, 994, 1237, 1241
HpyCH4III ACNGT 3 cut(s) 445, 1131, 1196
HpyCH4V TGCA 7 cut(s) 135, 608, 686, 707, 1085, 1090, 1126
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
HpyF3I CTNAG 6 cut(s) 305, 636, 1066, 1074, 1137, 1225
Hsp92II CATG 6 cut(s) 113, 668, 1055, 1111, 1117, 1156
Kpn2I TCCGGA 1 cut(s) 941
Ksp22I TGATCA 1 cut(s) 715
Kzo9I GATC 8 cut(s) 96, 207, 532, 585, 715, 1003, 1168, 1250
LmnI GCTCC 2 cut(s) 1051, 1164
Lsp1109I GCAGC 3 cut(s) 670, 880, 1102
LweI GCATC 7 cut(s) 61, 144, 265, 291, 695, 716, 768
MaeI CTAG 4 cut(s) 114, 204, 680, 929
MaeIII GTNAC 2 cut(s) 631, 862
MalI GATC 8 cut(s) 98, 209, 534, 587, 717, 1005, 1170, 1252
MboI GATC 8 cut(s) 96, 207, 532, 585, 715, 1003, 1168, 1250
MfeI CAATTG 1 cut(s) 449
MflI RGATCY 2 cut(s) 96, 1168
MhlI GDGCHC 1 cut(s) 743
MlyI GAGTC 7 cut(s) 385, 448, 622, 628, 856, 1148, 1266
MmeI TCCRAC 1 cut(s) 935
Mph1103I ATGCAT 1 cut(s) 137
MroI TCCGGA 1 cut(s) 941
MroXI GAANNNNTTC 1 cut(s) 1206
MseI TTAA 2 cut(s) 543, 779
MslI CAYNNNNRTG 2 cut(s) 663, 1112
MspA1I CMGCKG 1 cut(s) 437
MspCI CTTAAG 1 cut(s) 778
MspI CCGG 2 cut(s) 31, 942
MunI CAATTG 1 cut(s) 449
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeI CATATG 1 cut(s) 131
NdeII GATC 8 cut(s) 96, 207, 532, 585, 715, 1003, 1168, 1250
NlaIII CATG 6 cut(s) 113, 668, 1055, 1111, 1117, 1156
NlaIV GGNNCC 2 cut(s) 119, 357
NmeAIII GCCGAG 1 cut(s) 100
NmuCI GTSAC 2 cut(s) 631, 862
NsiI ATGCAT 1 cut(s) 137
NspI RCATGY 1 cut(s) 1111
PaeR7I CTCGAG 1 cut(s) 393
PciI ACATGT 1 cut(s) 1107
PdmI GAANNNNTTC 1 cut(s) 1206
PfeI GAWTC 7 cut(s) 5, 27, 88, 229, 619, 830, 1207
PflMI CCANNNNNTGG 1 cut(s) 340
PkrI GCNGC 4 cut(s) 62, 685, 895, 1092
PleI GAGTC 7 cut(s) 385, 448, 621, 628, 855, 1147, 1265
PpsI GAGTC 7 cut(s) 385, 448, 621, 628, 855, 1147, 1265
PscI ACATGT 1 cut(s) 1107
PshBI ATTAAT 1 cut(s) 543
Psp124BI GAGCTC 1 cut(s) 743
PspFI CCCAGC 1 cut(s) 433
PspN4I GGNNCC 2 cut(s) 119, 357
PspPI GGNCC 3 cut(s) 312, 355, 1275
PstNI CAGNNNCTG 1 cut(s) 1073
PsuI RGATCY 2 cut(s) 96, 1168
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 3 cut(s) 35, 641, 1112
RsaNI GTAC 3 cut(s) 34, 640, 1111
RseI CAYNNNNRTG 2 cut(s) 663, 1112
SacI GAGCTC 1 cut(s) 743
SalI GTCGAC 1 cut(s) 1103
SaqAI TTAA 2 cut(s) 543, 779
SatI GCNGC 4 cut(s) 61, 684, 894, 1091
Sau3AI GATC 8 cut(s) 96, 207, 532, 585, 715, 1003, 1168, 1250
Sau96I GGNCC 3 cut(s) 312, 355, 1275
ScaI AGTACT 1 cut(s) 641
SchI GAGTC 7 cut(s) 385, 448, 622, 628, 856, 1148, 1266
SduI GDGCHC 1 cut(s) 743
SfaNI GCATC 7 cut(s) 61, 144, 265, 291, 695, 716, 768
Sfr274I CTCGAG 1 cut(s) 393
SinI GGWCC 1 cut(s) 355
SlaI CTCGAG 1 cut(s) 393
SmiMI CAYNNNNRTG 2 cut(s) 663, 1112
SmlI CTYRAG 7 cut(s) 92, 250, 393, 672, 778, 799, 916
SmoI CTYRAG 7 cut(s) 92, 250, 393, 672, 778, 799, 916
SsiI CCGC 1 cut(s) 60
SspMI CTAG 4 cut(s) 114, 204, 680, 929
SstI GAGCTC 1 cut(s) 743
TaaI ACNGT 3 cut(s) 445, 1131, 1196
TaqI TCGA 7 cut(s) 394, 535, 581, 588, 837, 956, 1104
TaqII GACCGA 1 cut(s) 825
TatI WGTACW 2 cut(s) 639, 1110
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 7 cut(s) 5, 27, 88, 229, 619, 830, 1207
Tru1I TTAA 2 cut(s) 543, 779
Tru9I TTAA 2 cut(s) 543, 779
TscAI CASTG 3 cut(s) 421, 615, 874
TseFI GTSAC 2 cut(s) 631, 862
TseI GCWGC 3 cut(s) 683, 893, 1090
Tsp45I GTSAC 2 cut(s) 631, 862
TspDTI ATGAA 9 cut(s) 156, 258, 272, 741, 963, 993, 1010, 1076, 1169
TspRI CASTG 3 cut(s) 421, 615, 874
Van91I CCANNNNNTGG 1 cut(s) 340
Vha464I CTTAAG 1 cut(s) 778
VpaK11BI GGWCC 1 cut(s) 355
VspI ATTAAT 1 cut(s) 543
XapI RAATTY 5 cut(s) 43, 164, 236, 590, 886
XceI RCATGY 1 cut(s) 1111
XcmI CCANNNNNNNNNTGG 2 cut(s) 322, 434
XhoI CTCGAG 1 cut(s) 393
XmiI GTMKAC 2 cut(s) 156, 1104
XmnI GAANNNNTTC 1 cut(s) 1206
XspI CTAG 4 cut(s) 114, 204, 680, 929
ZrmI AGTACT 1 cut(s) 641
Zsp2I ATGCAT 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.