RLG00000034712

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012150
Physical Location & Seq
Reverse (-)
52707070 .. 52708347
1278 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034712

Sequence Viewer

Length: 1278 bp
ATGGAATCATTCAGTCAAACAACTGGATTCCGGTACAACCTGAATTCAAACACAACAGGCGGCGATGATGCCGAGTTTTCAGGGATTCTTGAGATCTATGCTCATCATGCTAGGAACCTTCACAACATATGCATCTATGAAAACCAAGATGTCTACGCAAAATTCTCTCTTACTTACAATCCAGATGAGACCCTCTCAACTAGGATCATCAATGGAGGTGGCAAGAATCCAGAATTCAATGAAAACTTGAGGATGAAAGTCACTCAAATTGATGCTGTTCTCAAATGTGAGATTTGGATGCTTAGCAGGGCCAGAAACTATATGGAAGACCAGCTCTTGGGATTTACTTTGGTCCCAATTTCGCAAGTGATTGGGAAAGGAAAGCTGACTCGAGATTATAGTCTTTCTTCCACTGACCTCTTCCATTCCCCAGCTGGAACTGTCCAATTGACTCTTTCTCTCATCCCATCTTTGCCTATCAAATTGTCTGCTAACTCATCCATAACTTCAGAAGTAGTATTGCTTGATCGAAAGAATTCAGAAGAAGTAATGTTAGACCCAGTTGAGTATTCGAGGATCGAATTTCCTGATATCAATGCAGTGAGTCAGAATCAGCAAATGGTGACTCAGTACTTTGACTTGGCAAAGCACAAGCATGGTTCTGAAGGACTAGTTGCATCATTTCTTCGTCTTGGTGCATCTCCTGATCAGGACTATGAAATGATTGTGAGCTCATCGCCTAATGAGAGCATCCACAACTCTGGCTTCTTAAGTTCCACCACAACAAGCTTGAGTGATGACCGAAACAACAATTCTGCTGATTCAATCGAGAAAAAGAGTCGTGTGGCTTCTGTCAGCAGTGGAGTAGCTCATCAAAATTTTGCTGCTTGTCCAGACACTCCAACCTCAAGAAAGGGAACAGAAGCCTCTTCCGGAGATGAAAAGTCGAGTTTCTTGGGCAAGGAAGATGAAGAGGAAGAGGAGATGAAGAAGGATAAAAAAGTTGGTTCAGGTCAATTTGGTCAGGTGTTTTCTGCTCCACATGGGAATATGAACTCAGAGGCTGAGCAATCTGCAATGCAGCAACAAATAGTCGACATGTACATGAGAAGTATGCAACAGTTTACTGAGTCTCTGGCAAACATGAAGCTCCCGATGGATCTTGATAAACCTGAATGTGAAGACCGTGGTGAAGTGATTCCGAACGGTAACAACTTAGAACTTGATAAAAAGAAGGATGGATCAAGAGTCTTCTATGGTAGCAGGGCCTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

426

Amino Acids

47.2

Weight (kDa)

4.96

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 28 - 132 8.2e-09 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12300
fragaria_vesca FvH4_3g27890 FvH4_3g27890 FvH4_3g27890
malus_domestica MD03G1166500.v1.1
prunus_persica Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1
pyrus_communis pycom03g12210
rosa_chinensis RchiOBHm_Chr5g0050621
rosa_laevigata RLG00000034712
rosa_multiflora Rmu_ssc0000350.1_g000030
rosa_roxburghii Rroxscaffold_1G00030180
rosa_rugosa Rorug05G0262100
rosa_samantha Rh5AG334400 Rh5BG343800 Rh5CG371600 Rh5DG356800
rosa_wichuraiana Rw5G031550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 337
AccI GTMKAC 2 cut(s) 153, 1095
AccIII TCCGGA 1 cut(s) 932
AciI CCGC 1 cut(s) 60
AclWI GGATC 4 cut(s) 212, 584, 1167, 1249
AcsI RAATTY 6 cut(s) 43, 161, 233, 535, 581, 877
AcuI CTGAAG 2 cut(s) 492, 684
AfaI GTAC 3 cut(s) 35, 632, 1103
AfiI CCNNNNNNNGG 1 cut(s) 337
AflII CTTAAG 1 cut(s) 769
AflIII ACRYGT 1 cut(s) 1098
AgsI TTSAA 3 cut(s) 48, 238, 825
AhlI ACTAGT 1 cut(s) 670
AluBI AGCT 7 cut(s) 334, 385, 434, 732, 789, 869, 1150
AluI AGCT 7 cut(s) 334, 385, 434, 732, 789, 869, 1150
Alw21I GWGCWC 1 cut(s) 734
Alw26I GTCTC 2 cut(s) 182, 1137
AlwI GGATC 4 cut(s) 212, 584, 1167, 1249
AlwNI CAGNNNCTG 1 cut(s) 1064
Ama87I CYCGRG 1 cut(s) 390
Aor13HI TCCGGA 1 cut(s) 932
AoxI GGCC 2 cut(s) 309, 1266
ApeKI GCWGC 2 cut(s) 884, 1081
ApoI RAATTY 6 cut(s) 43, 161, 233, 535, 581, 877
Asp700I GAANNNNTTC 2 cut(s) 535, 1197
AspS9I GGNCC 3 cut(s) 309, 352, 1266
AsuHPI GGTGA 2 cut(s) 634, 1202
AvaI CYCGRG 1 cut(s) 390
AvaII GGWCC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 734
BbsI GAAGAC 3 cut(s) 333, 1188, 1243
Bbv12I GWGCWC 1 cut(s) 734
BbvI GCAGC 2 cut(s) 871, 1093
BccI CCATC 3 cut(s) 475, 1150, 1232
BclI TGATCA 1 cut(s) 706
BcoDI GTCTC 2 cut(s) 182, 1137
BcuI ACTAGT 1 cut(s) 670
BfaI CTAG 3 cut(s) 111, 201, 671
BfrI CTTAAG 1 cut(s) 769
BglII AGATCT 1 cut(s) 93
BisI GCNGC 3 cut(s) 61, 885, 1082
BlpI GCTNAGC 2 cut(s) 302, 1065
BlsI GCNGC 3 cut(s) 62, 886, 1083
BmcAI AGTACT 1 cut(s) 632
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 390
BmgT120I GGNCC 3 cut(s) 309, 352, 1266
BmiI GGNNCC 2 cut(s) 116, 354
BmrI ACTGGG 1 cut(s) 554
BmsI GCATC 7 cut(s) 58, 141, 262, 288, 686, 707, 759
BmuI ACTGGG 1 cut(s) 554
BpiI GAAGAC 3 cut(s) 333, 1188, 1243
BplI GAGNNNNNCTC 2 cut(s) 179, 211
Bpu1102I GCTNAGC 2 cut(s) 302, 1065
BpuEI CTTGAG 4 cut(s) 110, 268, 811, 892
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 1186
BsaWI WCCGGW 2 cut(s) 30, 932
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse1I ACTGG 2 cut(s) 28, 560
Bse3DI GCAATG 1 cut(s) 1083
BseAI TCCGGA 1 cut(s) 932
BseDI CCNNGG 1 cut(s) 1186
BseGI GGATG 6 cut(s) 258, 303, 462, 497, 750, 1243
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMI GCAATG 1 cut(s) 1083
BseMII CTCAG 4 cut(s) 641, 1056, 1071, 1119
BseNI ACTGG 2 cut(s) 28, 560
BseRI GAGGAG 1 cut(s) 995
BseXI GCAGC 2 cut(s) 871, 1093
BseYI CCCAGC 1 cut(s) 430
BshFI GGCC 2 cut(s) 311, 1268
BsiHKAI GWGCWC 1 cut(s) 734
BsiHKCI CYCGRG 1 cut(s) 390
BsiSI CCGG 2 cut(s) 31, 933
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 1 cut(s) 337
BsmAI GTCTC 2 cut(s) 182, 1137
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 2 cut(s) 311, 1268
Bso31I GGTCTC 1 cut(s) 182
BsoBI CYCGRG 1 cut(s) 390
Bsp1286I GDGCHC 1 cut(s) 734
Bsp13I TCCGGA 1 cut(s) 932
Bsp1407I TGTACA 1 cut(s) 1101
Bsp143I GATC 7 cut(s) 93, 204, 526, 576, 706, 1159, 1241
Bsp1720I GCTNAGC 2 cut(s) 302, 1065
BspACI CCGC 1 cut(s) 60
BspANI GGCC 2 cut(s) 311, 1268
BspCNI CTCAG 4 cut(s) 640, 1057, 1070, 1120
BspEI TCCGGA 1 cut(s) 932
BspLI GGNNCC 2 cut(s) 116, 354
BspPI GGATC 4 cut(s) 212, 584, 1167, 1249
BspTI CTTAAG 1 cut(s) 769
BspTNI GGTCTC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 1083
BsrGI TGTACA 1 cut(s) 1101
BsrI ACTGG 2 cut(s) 28, 560
BssECI CCNNGG 1 cut(s) 1186
BssMI GATC 7 cut(s) 93, 204, 526, 576, 706, 1159, 1241
Bst4CI ACNGT 4 cut(s) 442, 1122, 1187, 1208
Bst6I CTCTTC 4 cut(s) 425, 934, 966, 972
BstAFI CTTAAG 1 cut(s) 769
BstAUI TGTACA 1 cut(s) 1101
BstDEI CTNAG 6 cut(s) 302, 627, 1057, 1065, 1128, 1216
BstDSI CCRYGG 1 cut(s) 1186
BstF5I GGATG 6 cut(s) 258, 303, 462, 497, 750, 1243
BstKTI GATC 7 cut(s) 96, 207, 529, 579, 709, 1162, 1244
BstMAI GTCTC 2 cut(s) 182, 1137
BstMBI GATC 7 cut(s) 93, 204, 526, 576, 706, 1159, 1241
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNSI RCATGY 1 cut(s) 1102
BstV1I GCAGC 2 cut(s) 871, 1093
BstV2I GAAGAC 3 cut(s) 333, 1188, 1243
BstX2I RGATCY 2 cut(s) 93, 1159
BstXI CCANNNNNNTGG 1 cut(s) 761
BstYI RGATCY 2 cut(s) 93, 1159
BsuRI GGCC 2 cut(s) 311, 1268
BtgI CCRYGG 1 cut(s) 1186
BtgZI GCGATG 2 cut(s) 78, 720
BtsCI GGATG 6 cut(s) 258, 303, 462, 497, 750, 1243
BtsI GCAGTG 2 cut(s) 606, 865
BtsIMutI CAGTG 3 cut(s) 411, 606, 865
CaiI CAGNNNCTG 1 cut(s) 1064
Cfr13I GGNCC 3 cut(s) 309, 352, 1266
Csp6I GTAC 3 cut(s) 34, 631, 1102
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviAII CATG 6 cut(s) 107, 656, 1043, 1099, 1105, 1144
CviQI GTAC 3 cut(s) 34, 631, 1102
DdeI CTNAG 6 cut(s) 302, 627, 1057, 1065, 1128, 1216
DpnI GATC 7 cut(s) 95, 206, 528, 578, 708, 1161, 1243
DpnII GATC 7 cut(s) 93, 204, 526, 576, 706, 1159, 1241
Eam1104I CTCTTC 4 cut(s) 425, 934, 966, 972
EarI CTCTTC 4 cut(s) 425, 934, 966, 972
Ecl136II GAGCTC 1 cut(s) 732
Eco24I GRGCYC 1 cut(s) 734
Eco31I GGTCTC 1 cut(s) 182
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 732
Eco57I CTGAAG 2 cut(s) 492, 684
Eco88I CYCGRG 1 cut(s) 390
EcoICRI GAGCTC 1 cut(s) 732
EcoO109I RGGNCCY 1 cut(s) 1266
EcoRI GAATTC 3 cut(s) 43, 233, 535
EcoRV GATATC 1 cut(s) 592
EcoT22I ATGCAT 1 cut(s) 134
EcoT38I GRGCYC 1 cut(s) 734
FaeI CATG 6 cut(s) 110, 659, 1046, 1102, 1108, 1147
FaqI GGGAC 1 cut(s) 338
FatI CATG 6 cut(s) 106, 655, 1042, 1098, 1104, 1143
FauNDI CATATG 1 cut(s) 128
FbaI TGATCA 1 cut(s) 706
FblI GTMKAC 2 cut(s) 153, 1095
Fnu4HI GCNGC 3 cut(s) 61, 885, 1082
FokI GGATG 6 cut(s) 265, 310, 449, 484, 737, 1250
FriOI GRGCYC 1 cut(s) 734
Fsp4HI GCNGC 3 cut(s) 61, 885, 1082
FspBI CTAG 3 cut(s) 111, 201, 671
GluI GCNGC 3 cut(s) 61, 885, 1082
GsaI CCCAGC 1 cut(s) 434
HaeIII GGCC 2 cut(s) 311, 1268
HapII CCGG 2 cut(s) 31, 933
Hin1II CATG 6 cut(s) 110, 659, 1046, 1102, 1108, 1147
HincII GTYRAC 1 cut(s) 1096
HindII GTYRAC 1 cut(s) 1096
HindIII AAGCTT 1 cut(s) 787
HpaII CCGG 2 cut(s) 31, 933
HphI GGTGA 2 cut(s) 634, 1202
Hpy166II GTNNAC 3 cut(s) 154, 1096, 1125
Hpy188I TCNGA 7 cut(s) 511, 541, 609, 664, 1060, 1203, 1277
Hpy8I GTNNAC 3 cut(s) 154, 1096, 1125
HpyAV CCTTC 4 cut(s) 128, 659, 985, 1228
HpyCH4III ACNGT 4 cut(s) 442, 1122, 1187, 1208
HpyCH4V TGCA 7 cut(s) 132, 599, 677, 698, 1076, 1081, 1117
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 6 cut(s) 302, 627, 1057, 1065, 1128, 1216
Hsp92II CATG 6 cut(s) 110, 659, 1046, 1102, 1108, 1147
Kpn2I TCCGGA 1 cut(s) 932
Ksp22I TGATCA 1 cut(s) 706
Kzo9I GATC 7 cut(s) 93, 204, 526, 576, 706, 1159, 1241
LmnI GCTCC 2 cut(s) 1042, 1155
Lsp1109I GCAGC 2 cut(s) 871, 1093
LweI GCATC 7 cut(s) 58, 141, 262, 288, 686, 707, 759
MaeI CTAG 3 cut(s) 111, 201, 671
MaeIII GTNAC 3 cut(s) 259, 622, 1208
MalI GATC 7 cut(s) 95, 206, 528, 578, 708, 1161, 1243
MboI GATC 7 cut(s) 93, 204, 526, 576, 706, 1159, 1241
MfeI CAATTG 1 cut(s) 446
MflI RGATCY 2 cut(s) 93, 1159
MhlI GDGCHC 1 cut(s) 734
MlyI GAGTC 7 cut(s) 382, 445, 613, 619, 847, 1139, 1257
MmeI TCCRAC 1 cut(s) 926
Mph1103I ATGCAT 1 cut(s) 134
MroI TCCGGA 1 cut(s) 932
MroXI GAANNNNTTC 2 cut(s) 535, 1197
MseI TTAA 1 cut(s) 770
MslI CAYNNNNRTG 2 cut(s) 654, 1103
MspA1I CMGCKG 1 cut(s) 434
MspCI CTTAAG 1 cut(s) 769
MspI CCGG 2 cut(s) 31, 933
MunI CAATTG 1 cut(s) 446
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeI CATATG 1 cut(s) 128
NdeII GATC 7 cut(s) 93, 204, 526, 576, 706, 1159, 1241
NlaIII CATG 6 cut(s) 110, 659, 1046, 1102, 1108, 1147
NlaIV GGNNCC 2 cut(s) 116, 354
NmeAIII GCCGAG 1 cut(s) 97
NmuCI GTSAC 2 cut(s) 259, 622
NsiI ATGCAT 1 cut(s) 134
NspI RCATGY 1 cut(s) 1102
PaeR7I CTCGAG 1 cut(s) 390
PciI ACATGT 1 cut(s) 1098
PdmI GAANNNNTTC 2 cut(s) 535, 1197
PfeI GAWTC 7 cut(s) 5, 27, 85, 226, 610, 821, 1198
PflMI CCANNNNNTGG 1 cut(s) 337
PkrI GCNGC 3 cut(s) 62, 886, 1083
PleI GAGTC 7 cut(s) 382, 445, 612, 619, 846, 1138, 1256
PpsI GAGTC 7 cut(s) 382, 445, 612, 619, 846, 1138, 1256
PscI ACATGT 1 cut(s) 1098
Psp124BI GAGCTC 1 cut(s) 734
PspFI CCCAGC 1 cut(s) 430
PspN4I GGNNCC 2 cut(s) 116, 354
PspPI GGNCC 3 cut(s) 309, 352, 1266
PstNI CAGNNNCTG 1 cut(s) 1064
PsuI RGATCY 2 cut(s) 93, 1159
PvuII CAGCTG 1 cut(s) 434
RsaI GTAC 3 cut(s) 35, 632, 1103
RsaNI GTAC 3 cut(s) 34, 631, 1102
RseI CAYNNNNRTG 2 cut(s) 654, 1103
SacI GAGCTC 1 cut(s) 734
SalI GTCGAC 1 cut(s) 1094
SaqAI TTAA 1 cut(s) 770
SatI GCNGC 3 cut(s) 61, 885, 1082
Sau3AI GATC 7 cut(s) 93, 204, 526, 576, 706, 1159, 1241
Sau96I GGNCC 3 cut(s) 309, 352, 1266
ScaI AGTACT 1 cut(s) 632
SchI GAGTC 7 cut(s) 382, 445, 613, 619, 847, 1139, 1257
SduI GDGCHC 1 cut(s) 734
SfaNI GCATC 7 cut(s) 58, 141, 262, 288, 686, 707, 759
Sfr274I CTCGAG 1 cut(s) 390
SinI GGWCC 1 cut(s) 352
SlaI CTCGAG 1 cut(s) 390
SmiMI CAYNNNNRTG 2 cut(s) 654, 1103
SmlI CTYRAG 6 cut(s) 89, 247, 390, 769, 790, 907
SmoI CTYRAG 6 cut(s) 89, 247, 390, 769, 790, 907
SpeI ACTAGT 1 cut(s) 670
SsiI CCGC 1 cut(s) 60
SspMI CTAG 3 cut(s) 111, 201, 671
SstI GAGCTC 1 cut(s) 734
TaaI ACNGT 4 cut(s) 442, 1122, 1187, 1208
TaqI TCGA 7 cut(s) 391, 529, 572, 579, 828, 947, 1095
TaqII GACCGA 1 cut(s) 816
TatI WGTACW 2 cut(s) 630, 1101
TauI GCSGC 1 cut(s) 63
TfiI GAWTC 7 cut(s) 5, 27, 85, 226, 610, 821, 1198
Tru1I TTAA 1 cut(s) 770
Tru9I TTAA 1 cut(s) 770
TscAI CASTG 3 cut(s) 418, 606, 865
TseFI GTSAC 2 cut(s) 259, 622
TseI GCWGC 2 cut(s) 884, 1081
Tsp45I GTSAC 2 cut(s) 259, 622
TspDTI ATGAA 9 cut(s) 153, 255, 269, 732, 954, 984, 1001, 1067, 1160
TspRI CASTG 3 cut(s) 418, 606, 865
Van91I CCANNNNNTGG 1 cut(s) 337
Vha464I CTTAAG 1 cut(s) 769
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 6 cut(s) 43, 161, 233, 535, 581, 877
XceI RCATGY 1 cut(s) 1102
XcmI CCANNNNNNNNNTGG 2 cut(s) 319, 431
XhoI CTCGAG 1 cut(s) 390
XmiI GTMKAC 2 cut(s) 153, 1095
XmnI GAANNNNTTC 2 cut(s) 535, 1197
XspI CTAG 3 cut(s) 111, 201, 671
ZrmI AGTACT 1 cut(s) 632
Zsp2I ATGCAT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.