Rh5DG356800

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0012150
Physical Location & Seq
Reverse (-)
53049734 .. 53051720
1987 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG356800.1

Sequence Viewer

Length: 1281 bp
ATGGAATCATTCAGTCAAACAACTGGATTCCGGTACAACCTGAATCCAAACACAACAGGCGGCGATGATGCCGAGTTTTCAGGGATTCTTGACATCTATGCTCATCATGCTAGGAACCTTCACAACATATGCATCTATGAAAACCAAGATGTCTACGCAAAATTCTCTCTTACTTACAATCCAGATGAGACCCTCTCAACTAGGATCATCAATGGAGGTGGCAAGAATCCAGAATTCAATGAAAACTTGAGGATGAAAATCACTCAAATTGATGCTGTTCTCAAATGTGAGATTTGGATGCTTAGCAGGGCCAGAAACTATATGGAAGACCAGCTCTTGGGATTTACTTTGGTCCCAATTTCGCAAGTGATTGGGAAAGGAAAGCTGACTCGAGATTACAGTCTTTCTTCCACTGACCTCTTCCATTCCCCAGCTGGAACCGTCCAATTGACTCTCTCTCTCATCCCATCTTTGCCTATCAAATTGTCTGCTAACTCATCCATAACTTCAGAAGTAGTATTGCTTGGTCGAAAGAATTCAGAAGAAGTAATGTTAGACCCAGTTGAGTATTCGAGGATCGAATTCCCTGATATCAATGCAGTGAGTCAGAATCAGCAAATGGTGACTCAGTACTTTGACTTGGCAAAGCACAAGCATGGTTCTCAAGGACTAGCTGCATCATTTCTTCGTCTTGGTGCATCTCCTGATCAGGACTATGAAATGATTGTGAGCTCATCGCCGCCTAATGAGAGCATCCACAACTCTGGCTTCTTAAGTTCCACCACAACAAGCTTGAGTGATGACCGGAACAACAATTCTGCTGATTCAGTCGAGCAAAAGAGTCGTGTGGCTTCTGTCACCAGTGGAGTAGCTCATCAAAATTTTGCTGCTTGTCCAGACACTCCAACCTCAAGAAAGGGAATAGAAGCCTCTTCCGGAGATGAAAAGTCGAGTTTCTTGGGCAAGGAAGATGAAGAGGAAGAGGAGATGAAGAAGGATCAAAAAGTTGGTTCAGGTCAATTTGGTCAGGTGTATTCTGCTCCACATGGGAATATGAACTCAGAGGCTGAGCAATCTGCAATGCAGCAACAAATAGTCGACATGTACATGAGAAGTATGCAACAGTTTACTGAGTCTCTGGCAAACATGAAGCTCCCGATGGATCTTGATAAACCTGAATGTGAAGACCGTGGTGAAGTGATTCCGAACAACAACAACTTAGAACTTGATAAAAAGAAGGATGGATCAAGAGTCTTCTATGGTAGCAGGGCCTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

426

Amino Acids

47.31

Weight (kDa)

4.94

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 29 - 132 2.8e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12300
fragaria_vesca FvH4_3g27890 FvH4_3g27890 FvH4_3g27890
malus_domestica MD03G1166500.v1.1
prunus_persica Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1
pyrus_communis pycom03g12210
rosa_chinensis RchiOBHm_Chr5g0050621
rosa_laevigata RLG00000034712
rosa_multiflora Rmu_ssc0000350.1_g000030
rosa_roxburghii Rroxscaffold_1G00030180
rosa_rugosa Rorug05G0262100
rosa_samantha Rh5AG334400 Rh5BG343800 Rh5CG371600 Rh5DG356800
rosa_wichuraiana Rw5G031550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 337
AccI GTMKAC 2 cut(s) 153, 1098
AccIII TCCGGA 1 cut(s) 935
AciI CCGC 2 cut(s) 60, 740
AclWI GGATC 5 cut(s) 212, 584, 1005, 1170, 1252
AcsI RAATTY 5 cut(s) 161, 233, 535, 581, 880
AcuI CTGAAG 1 cut(s) 492
AfaI GTAC 3 cut(s) 35, 632, 1106
AfiI CCNNNNNNNGG 1 cut(s) 337
AflII CTTAAG 1 cut(s) 772
AflIII ACRYGT 1 cut(s) 1101
AgsI TTSAA 1 cut(s) 238
AluBI AGCT 8 cut(s) 334, 385, 434, 674, 732, 792, 872, 1153
AluI AGCT 8 cut(s) 334, 385, 434, 674, 732, 792, 872, 1153
Alw21I GWGCWC 1 cut(s) 734
Alw26I GTCTC 2 cut(s) 182, 1140
AlwI GGATC 5 cut(s) 212, 584, 1005, 1170, 1252
AlwNI CAGNNNCTG 1 cut(s) 1067
Ama87I CYCGRG 1 cut(s) 390
Aor13HI TCCGGA 1 cut(s) 935
AoxI GGCC 2 cut(s) 309, 1269
ApeKI GCWGC 3 cut(s) 674, 887, 1084
ApoI RAATTY 5 cut(s) 161, 233, 535, 581, 880
Asp700I GAANNNNTTC 2 cut(s) 535, 1200
AspS9I GGNCC 3 cut(s) 309, 352, 1269
AsuHPI GGTGA 3 cut(s) 634, 850, 1205
AvaI CYCGRG 1 cut(s) 390
AvaII GGWCC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 734
BbsI GAAGAC 3 cut(s) 333, 1191, 1246
Bbv12I GWGCWC 1 cut(s) 734
BbvI GCAGC 3 cut(s) 661, 874, 1096
BccI CCATC 3 cut(s) 475, 1153, 1235
BcgI CGANNNNNNTGC 2 cut(s) 824, 858
BclI TGATCA 1 cut(s) 706
BcoDI GTCTC 2 cut(s) 182, 1140
BfaI CTAG 3 cut(s) 111, 201, 671
BfrI CTTAAG 1 cut(s) 772
BisI GCNGC 5 cut(s) 61, 675, 740, 888, 1085
BlpI GCTNAGC 2 cut(s) 302, 1068
BlsI GCNGC 5 cut(s) 62, 676, 741, 889, 1086
BmcAI AGTACT 1 cut(s) 632
Bme18I GGWCC 1 cut(s) 352
BmeT110I CYCGRG 1 cut(s) 390
BmgT120I GGNCC 3 cut(s) 309, 352, 1269
BmiI GGNNCC 3 cut(s) 116, 354, 439
BmrI ACTGGG 1 cut(s) 554
BmsI GCATC 7 cut(s) 58, 141, 262, 288, 686, 707, 762
BmuI ACTGGG 1 cut(s) 554
BpiI GAAGAC 3 cut(s) 333, 1191, 1246
BplI GAGNNNNNCTC 2 cut(s) 179, 211
Bpu1102I GCTNAGC 2 cut(s) 302, 1068
BpuEI CTTGAG 4 cut(s) 268, 648, 814, 895
BsaBI GATNNNNATC 1 cut(s) 257
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 1189
BsaWI WCCGGW 3 cut(s) 30, 804, 935
Bsc4I CCNNNNNNNGG 1 cut(s) 337
Bse1I ACTGG 3 cut(s) 28, 560, 861
Bse3DI GCAATG 1 cut(s) 1086
Bse8I GATNNNNATC 1 cut(s) 257
BseAI TCCGGA 1 cut(s) 935
BseDI CCNNGG 1 cut(s) 1189
BseGI GGATG 6 cut(s) 258, 303, 462, 497, 753, 1246
BseJI GATNNNNATC 1 cut(s) 257
BseLI CCNNNNNNNGG 1 cut(s) 337
BseMI GCAATG 1 cut(s) 1086
BseMII CTCAG 4 cut(s) 641, 1059, 1074, 1122
BseNI ACTGG 3 cut(s) 28, 560, 861
BseRI GAGGAG 1 cut(s) 998
BseXI GCAGC 3 cut(s) 661, 874, 1096
BseYI CCCAGC 1 cut(s) 430
BshFI GGCC 2 cut(s) 311, 1271
BsiHKAI GWGCWC 1 cut(s) 734
BsiHKCI CYCGRG 1 cut(s) 390
BsiSI CCGG 3 cut(s) 31, 805, 936
BslFI GGGAC 1 cut(s) 338
BslI CCNNNNNNNGG 1 cut(s) 337
BsmAI GTCTC 2 cut(s) 182, 1140
BsmFI GGGAC 1 cut(s) 338
BsnI GGCC 2 cut(s) 311, 1271
Bso31I GGTCTC 1 cut(s) 182
BsoBI CYCGRG 1 cut(s) 390
Bsp1286I GDGCHC 1 cut(s) 734
Bsp13I TCCGGA 1 cut(s) 935
Bsp1407I TGTACA 1 cut(s) 1104
Bsp143I GATC 6 cut(s) 204, 576, 706, 997, 1162, 1244
Bsp1720I GCTNAGC 2 cut(s) 302, 1068
BspACI CCGC 2 cut(s) 60, 740
BspANI GGCC 2 cut(s) 311, 1271
BspCNI CTCAG 4 cut(s) 640, 1060, 1073, 1123
BspEI TCCGGA 1 cut(s) 935
BspLI GGNNCC 3 cut(s) 116, 354, 439
BspPI GGATC 5 cut(s) 212, 584, 1005, 1170, 1252
BspTI CTTAAG 1 cut(s) 772
BspTNI GGTCTC 1 cut(s) 182
BsrDI GCAATG 1 cut(s) 1086
BsrGI TGTACA 1 cut(s) 1104
BsrI ACTGG 3 cut(s) 28, 560, 861
BssECI CCNNGG 1 cut(s) 1189
BssMI GATC 6 cut(s) 204, 576, 706, 997, 1162, 1244
Bst4CI ACNGT 4 cut(s) 401, 442, 1125, 1190
Bst6I CTCTTC 4 cut(s) 425, 937, 969, 975
BstAFI CTTAAG 1 cut(s) 772
BstAUI TGTACA 1 cut(s) 1104
BstDEI CTNAG 6 cut(s) 302, 627, 1060, 1068, 1131, 1219
BstDSI CCRYGG 1 cut(s) 1189
BstF5I GGATG 6 cut(s) 258, 303, 462, 497, 753, 1246
BstKTI GATC 6 cut(s) 207, 579, 709, 1000, 1165, 1247
BstMAI GTCTC 2 cut(s) 182, 1140
BstMBI GATC 6 cut(s) 204, 576, 706, 997, 1162, 1244
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstNSI RCATGY 1 cut(s) 1105
BstV1I GCAGC 3 cut(s) 661, 874, 1096
BstV2I GAAGAC 3 cut(s) 333, 1191, 1246
BstX2I RGATCY 1 cut(s) 1162
BstXI CCANNNNNNTGG 1 cut(s) 764
BstYI RGATCY 1 cut(s) 1162
BsuRI GGCC 2 cut(s) 311, 1271
BtgI CCRYGG 1 cut(s) 1189
BtgZI GCGATG 2 cut(s) 78, 720
BtsCI GGATG 6 cut(s) 258, 303, 462, 497, 753, 1246
BtsI GCAGTG 1 cut(s) 606
BtsIMutI CAGTG 3 cut(s) 411, 606, 868
CaiI CAGNNNCTG 1 cut(s) 1067
Cfr13I GGNCC 3 cut(s) 309, 352, 1269
Csp6I GTAC 3 cut(s) 34, 631, 1105
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviAII CATG 6 cut(s) 107, 656, 1046, 1102, 1108, 1147
CviQI GTAC 3 cut(s) 34, 631, 1105
DdeI CTNAG 6 cut(s) 302, 627, 1060, 1068, 1131, 1219
DpnI GATC 6 cut(s) 206, 578, 708, 999, 1164, 1246
DpnII GATC 6 cut(s) 204, 576, 706, 997, 1162, 1244
Eam1104I CTCTTC 4 cut(s) 425, 937, 969, 975
EarI CTCTTC 4 cut(s) 425, 937, 969, 975
Ecl136II GAGCTC 1 cut(s) 732
Eco24I GRGCYC 1 cut(s) 734
Eco31I GGTCTC 1 cut(s) 182
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 352
Eco53kI GAGCTC 1 cut(s) 732
Eco57I CTGAAG 1 cut(s) 492
Eco88I CYCGRG 1 cut(s) 390
EcoICRI GAGCTC 1 cut(s) 732
EcoO109I RGGNCCY 1 cut(s) 1269
EcoRI GAATTC 3 cut(s) 233, 535, 581
EcoRV GATATC 1 cut(s) 592
EcoT22I ATGCAT 1 cut(s) 134
EcoT38I GRGCYC 1 cut(s) 734
FaeI CATG 6 cut(s) 110, 659, 1049, 1105, 1111, 1150
FaqI GGGAC 1 cut(s) 338
FatI CATG 6 cut(s) 106, 655, 1045, 1101, 1107, 1146
FauNDI CATATG 1 cut(s) 128
FbaI TGATCA 1 cut(s) 706
FblI GTMKAC 2 cut(s) 153, 1098
Fnu4HI GCNGC 5 cut(s) 61, 675, 740, 888, 1085
FokI GGATG 6 cut(s) 265, 310, 449, 484, 740, 1253
FriOI GRGCYC 1 cut(s) 734
Fsp4HI GCNGC 5 cut(s) 61, 675, 740, 888, 1085
FspBI CTAG 3 cut(s) 111, 201, 671
GluI GCNGC 5 cut(s) 61, 675, 740, 888, 1085
GsaI CCCAGC 1 cut(s) 434
HaeIII GGCC 2 cut(s) 311, 1271
HapII CCGG 3 cut(s) 31, 805, 936
Hin1II CATG 6 cut(s) 110, 659, 1049, 1105, 1111, 1150
HincII GTYRAC 1 cut(s) 1099
HindII GTYRAC 1 cut(s) 1099
HindIII AAGCTT 1 cut(s) 790
HpaII CCGG 3 cut(s) 31, 805, 936
HphI GGTGA 3 cut(s) 634, 850, 1205
Hpy166II GTNNAC 3 cut(s) 154, 1099, 1128
Hpy188I TCNGA 6 cut(s) 511, 541, 609, 1063, 1206, 1280
Hpy8I GTNNAC 3 cut(s) 154, 1099, 1128
HpyAV CCTTC 3 cut(s) 128, 988, 1231
HpyCH4III ACNGT 4 cut(s) 401, 442, 1125, 1190
HpyCH4V TGCA 7 cut(s) 132, 599, 677, 698, 1079, 1084, 1120
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 6 cut(s) 302, 627, 1060, 1068, 1131, 1219
Hsp92II CATG 6 cut(s) 110, 659, 1049, 1105, 1111, 1150
Kpn2I TCCGGA 1 cut(s) 935
Ksp22I TGATCA 1 cut(s) 706
Kzo9I GATC 6 cut(s) 204, 576, 706, 997, 1162, 1244
LmnI GCTCC 2 cut(s) 1045, 1158
Lsp1109I GCAGC 3 cut(s) 661, 874, 1096
LweI GCATC 7 cut(s) 58, 141, 262, 288, 686, 707, 762
MaeI CTAG 3 cut(s) 111, 201, 671
MaeIII GTNAC 2 cut(s) 622, 856
MalI GATC 6 cut(s) 206, 578, 708, 999, 1164, 1246
MboI GATC 6 cut(s) 204, 576, 706, 997, 1162, 1244
MfeI CAATTG 1 cut(s) 446
MflI RGATCY 1 cut(s) 1162
MhlI GDGCHC 1 cut(s) 734
MlyI GAGTC 7 cut(s) 382, 445, 613, 619, 850, 1142, 1260
MmeI TCCRAC 1 cut(s) 929
Mph1103I ATGCAT 1 cut(s) 134
MroI TCCGGA 1 cut(s) 935
MroXI GAANNNNTTC 2 cut(s) 535, 1200
MseI TTAA 1 cut(s) 773
MslI CAYNNNNRTG 2 cut(s) 654, 1106
MspA1I CMGCKG 1 cut(s) 434
MspCI CTTAAG 1 cut(s) 772
MspI CCGG 3 cut(s) 31, 805, 936
MunI CAATTG 1 cut(s) 446
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeI CATATG 1 cut(s) 128
NdeII GATC 6 cut(s) 204, 576, 706, 997, 1162, 1244
NlaIII CATG 6 cut(s) 110, 659, 1049, 1105, 1111, 1150
NlaIV GGNNCC 3 cut(s) 116, 354, 439
NmeAIII GCCGAG 1 cut(s) 97
NmuCI GTSAC 2 cut(s) 622, 856
NsiI ATGCAT 1 cut(s) 134
NspI RCATGY 1 cut(s) 1105
PaeR7I CTCGAG 1 cut(s) 390
PciI ACATGT 1 cut(s) 1101
PdmI GAANNNNTTC 2 cut(s) 535, 1200
PfeI GAWTC 8 cut(s) 5, 27, 43, 85, 226, 610, 824, 1201
PflMI CCANNNNNTGG 1 cut(s) 337
PkrI GCNGC 5 cut(s) 62, 676, 741, 889, 1086
PleI GAGTC 7 cut(s) 382, 445, 612, 619, 849, 1141, 1259
PpsI GAGTC 7 cut(s) 382, 445, 612, 619, 849, 1141, 1259
PscI ACATGT 1 cut(s) 1101
Psp124BI GAGCTC 1 cut(s) 734
PspFI CCCAGC 1 cut(s) 430
PspN4I GGNNCC 3 cut(s) 116, 354, 439
PspPI GGNCC 3 cut(s) 309, 352, 1269
PstNI CAGNNNCTG 1 cut(s) 1067
PsuI RGATCY 1 cut(s) 1162
PvuII CAGCTG 1 cut(s) 434
RsaI GTAC 3 cut(s) 35, 632, 1106
RsaNI GTAC 3 cut(s) 34, 631, 1105
RseI CAYNNNNRTG 2 cut(s) 654, 1106
SacI GAGCTC 1 cut(s) 734
SalI GTCGAC 1 cut(s) 1097
SaqAI TTAA 1 cut(s) 773
SatI GCNGC 5 cut(s) 61, 675, 740, 888, 1085
Sau3AI GATC 6 cut(s) 204, 576, 706, 997, 1162, 1244
Sau96I GGNCC 3 cut(s) 309, 352, 1269
ScaI AGTACT 1 cut(s) 632
SchI GAGTC 7 cut(s) 382, 445, 613, 619, 850, 1142, 1260
SduI GDGCHC 1 cut(s) 734
SfaNI GCATC 7 cut(s) 58, 141, 262, 288, 686, 707, 762
Sfr274I CTCGAG 1 cut(s) 390
SinI GGWCC 1 cut(s) 352
SlaI CTCGAG 1 cut(s) 390
SmiMI CAYNNNNRTG 2 cut(s) 654, 1106
SmlI CTYRAG 6 cut(s) 247, 390, 663, 772, 793, 910
SmoI CTYRAG 6 cut(s) 247, 390, 663, 772, 793, 910
SsiI CCGC 2 cut(s) 60, 740
SspMI CTAG 3 cut(s) 111, 201, 671
SstI GAGCTC 1 cut(s) 734
TaaI ACNGT 4 cut(s) 401, 442, 1125, 1190
TaqI TCGA 7 cut(s) 391, 529, 572, 579, 831, 950, 1098
TatI WGTACW 2 cut(s) 630, 1104
TauI GCSGC 2 cut(s) 63, 742
TfiI GAWTC 8 cut(s) 5, 27, 43, 85, 226, 610, 824, 1201
Tru1I TTAA 1 cut(s) 773
Tru9I TTAA 1 cut(s) 773
TscAI CASTG 3 cut(s) 418, 606, 868
TseFI GTSAC 2 cut(s) 622, 856
TseI GCWGC 3 cut(s) 674, 887, 1084
Tsp45I GTSAC 2 cut(s) 622, 856
TspDTI ATGAA 9 cut(s) 153, 255, 269, 732, 957, 987, 1004, 1070, 1163
TspRI CASTG 3 cut(s) 418, 606, 868
Van91I CCANNNNNTGG 1 cut(s) 337
Vha464I CTTAAG 1 cut(s) 772
VpaK11BI GGWCC 1 cut(s) 352
XapI RAATTY 5 cut(s) 161, 233, 535, 581, 880
XceI RCATGY 1 cut(s) 1105
XcmI CCANNNNNNNNNTGG 2 cut(s) 319, 431
XhoI CTCGAG 1 cut(s) 390
XmiI GTMKAC 2 cut(s) 153, 1098
XmnI GAANNNNTTC 2 cut(s) 535, 1200
XspI CTAG 3 cut(s) 111, 201, 671
ZrmI AGTACT 1 cut(s) 632
Zsp2I ATGCAT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.