FvH4_3g27890

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0012150
Physical Location & Seq
Reverse (-)
20924100 .. 20926262
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g27890.t1

Sequence Viewer

Length: 1272 bp
ATGGAATCATTCAGTCAAACTGGGTTCCGGTACAACCTGGATTCGAACACAATAGGTGGTGATGATACAGAGTTTTCAGGGATTCTTGAGATCTATGCTCATCATGCTAGGAACCTTCACAACATATGCATATATGAAAAGCAAGATGTCTATGCAAAGTTCTCTCTTACTTACAATCCAGATGAGGCCCTCTCAACTAGGATCATCAATGGAGGTGGCAAGAATCCAGAATTCAATGAAAACTTGAGGATGAGGATTACTCAAATTGATGCTGTTCTCAAATGTGAGATTTGGATGCTTAGTAGGGCCAGAAGCTACATGGAGGACCAGCTCTTGGGATTCACTTTGGTTCCAATTTCGCAAGTGACTGGCAAAGGAAAGCTGACTCAAGATTATGGTCTTTCTTCCACTGATCTCTTCCATTCCCCAGCTGGCACTGTGCAACTGACTCTTTCTCTTATCCCATCTTCGCCTATCAAATCGTCTGCTAACTCATCCATAACTTCAGAAGTAGTATTGCTTGATCGAAAGATCTCAGAAGAAGTAATGTTAGACCCAGTTGAGTATACAAGGATCGAATTCCCTGACATCAATGTAGTGAGTCAGAATCAGCAAATGGTGACGGAGTACTTTGACTTGGCAAAGCACAAGCATGATTCTGAAGGACTAGTTGCCTCGTTTCTTCGTCTTGGTGCATCTCCTGATCAGGACTGTGAAATGCTCGTGAGCTCATCGCCCAATGAGAGCATACAAAACTCTGGCTTCTTAAGTTCCACCACAAGAAGCTTGAGTGATGACCGAAACAACAACAATGCTGATTCAATCGAGAAAAAGAATTGTGTGGCTTCTGTCACCAATGGAGCAGCTAATCAAAATTGTGCTGCTTGTCCAGACACTCCAACCTCAAGAAAGGGAAGAGAGGCATCTTCTGGAGATGAAAAGTTGAGTTTTCTGGGCAAGGAAGAGGAAGAGGAAGAGGAGATGAAGAAGGAACATGAAGTTGGTCCGGGCCCATTTGGTCAGGTGTATTCTGCTCCACTCGGGAATATGAACTCAGAGGCCGAGCAATCTGCAATGCAGCAACAAATAGTTGACATGTACATGAGAAGTATGCAACAGTTTACCGAGTCTCTGGCAAACATGAAGCTCCCGATGGATCTTGATAAACCCAAATGTGAAGACGGTGGTGAAGTGATTCCGAACAGCAACTTAGAACTTGACAAAAAGAAGGATGGATCAAGAGTCTTCTATGGTAGCAGGGCCTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

424

Amino Acids

46.96

Weight (kDa)

4.76

Isoelectric Point (pI)

47.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 27 - 131 1e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12300
fragaria_vesca FvH4_3g27890 FvH4_3g27890 FvH4_3g27890
malus_domestica MD03G1166500.v1.1
prunus_persica Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1
pyrus_communis pycom03g12210
rosa_chinensis RchiOBHm_Chr5g0050621
rosa_laevigata RLG00000034712
rosa_multiflora Rmu_ssc0000350.1_g000030
rosa_roxburghii Rroxscaffold_1G00030180
rosa_rugosa Rorug05G0262100
rosa_samantha Rh5AG334400 Rh5BG343800 Rh5CG371600 Rh5DG356800
rosa_wichuraiana Rw5G031550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 334
AccI GTMKAC 1 cut(s) 566
AclWI GGATC 4 cut(s) 209, 581, 1164, 1243
AcsI RAATTY 2 cut(s) 230, 578
AcuI CTGAAG 2 cut(s) 489, 681
AfaI GTAC 3 cut(s) 32, 629, 1100
AfiI CCNNNNNNNGG 1 cut(s) 334
AflII CTTAAG 1 cut(s) 766
AflIII ACRYGT 1 cut(s) 1095
AgsI TTSAA 2 cut(s) 235, 822
AhlI ACTAGT 1 cut(s) 667
AjnI CCWGG 1 cut(s) 36
AjuI GAANNNNNNNTTGG 2 cut(s) 984, 1016
AluBI AGCT 8 cut(s) 315, 331, 382, 431, 729, 786, 866, 1147
AluI AGCT 8 cut(s) 315, 331, 382, 431, 729, 786, 866, 1147
Alw21I GWGCWC 1 cut(s) 731
Alw26I GTCTC 1 cut(s) 1134
AlwI GGATC 4 cut(s) 209, 581, 1164, 1243
Ama87I CYCGRG 1 cut(s) 1040
AoxI GGCC 5 cut(s) 186, 306, 1009, 1059, 1260
ApaI GGGCCC 1 cut(s) 1013
ApeKI GCWGC 3 cut(s) 863, 881, 1078
ApoI RAATTY 2 cut(s) 230, 578
Asp700I GAANNNNTTC 1 cut(s) 1194
AspS9I GGNCC 7 cut(s) 187, 306, 325, 1004, 1009, 1010, 1260
AsuC2I CCSGG 1 cut(s) 1008
AsuHPI GGTGA 4 cut(s) 71, 631, 844, 1199
AsuII TTCGAA 1 cut(s) 44
AvaI CYCGRG 1 cut(s) 1040
AvaII GGWCC 2 cut(s) 325, 1004
BaeGI GKGCMC 1 cut(s) 1013
BanII GRGCYC 2 cut(s) 731, 1013
BauI CACGAG 1 cut(s) 722
BbsI GAAGAC 2 cut(s) 1185, 1237
Bbv12I GWGCWC 1 cut(s) 731
BbvI GCAGC 3 cut(s) 868, 875, 1090
BccI CCATC 3 cut(s) 472, 1147, 1226
BcgI CGANNNNNNTGC 2 cut(s) 1052, 1086
BciT130I CCWGG 1 cut(s) 38
BclI TGATCA 1 cut(s) 703
BcnI CCSGG 1 cut(s) 1008
BcoDI GTCTC 1 cut(s) 1134
BcuI ACTAGT 1 cut(s) 667
BfaI CTAG 3 cut(s) 108, 198, 668
BfrI CTTAAG 1 cut(s) 766
BglII AGATCT 2 cut(s) 90, 531
BisI GCNGC 3 cut(s) 864, 882, 1079
BlsI GCNGC 3 cut(s) 865, 883, 1080
BmcAI AGTACT 1 cut(s) 629
Bme1390I CCNGG 2 cut(s) 38, 1008
Bme18I GGWCC 2 cut(s) 325, 1004
BmeT110I CYCGRG 1 cut(s) 1040
BmgT120I GGNCC 7 cut(s) 187, 306, 325, 1004, 1009, 1010, 1260
BmiI GGNNCC 4 cut(s) 26, 113, 351, 1011
BmrFI CCNGG 2 cut(s) 38, 1008
BmrI ACTGGG 2 cut(s) 30, 551
BmsI GCATC 4 cut(s) 259, 285, 704, 932
BmuI ACTGGG 2 cut(s) 30, 551
BpiI GAAGAC 2 cut(s) 1185, 1237
BplI GAGNNNNNCTC 4 cut(s) 176, 208, 244, 276
BpmI CTGGAG 1 cut(s) 951
Bpu14I TTCGAA 1 cut(s) 44
BpuEI CTTGAG 5 cut(s) 107, 265, 372, 808, 889
BpuMI CCSGG 1 cut(s) 1008
BsaWI WCCGGW 1 cut(s) 27
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse1I ACTGG 3 cut(s) 25, 373, 557
Bse3DI GCAATG 1 cut(s) 1080
BseBI CCWGG 1 cut(s) 38
BseGI GGATG 4 cut(s) 255, 300, 494, 1237
BseLI CCNNNNNNNGG 1 cut(s) 334
BseMI GCAATG 1 cut(s) 1080
BseMII CTCAG 2 cut(s) 549, 1068
BseNI ACTGG 3 cut(s) 25, 373, 557
BseRI GAGGAG 1 cut(s) 992
BseSI GKGCMC 1 cut(s) 1013
BseXI GCAGC 3 cut(s) 868, 875, 1090
BseYI CCCAGC 1 cut(s) 427
BshFI GGCC 5 cut(s) 188, 308, 1011, 1061, 1262
BsiHKAI GWGCWC 1 cut(s) 731
BsiHKCI CYCGRG 1 cut(s) 1040
BsiSI CCGG 2 cut(s) 28, 1007
BslI CCNNNNNNNGG 1 cut(s) 334
BsmAI GTCTC 1 cut(s) 1134
BsnI GGCC 5 cut(s) 188, 308, 1011, 1061, 1262
BsoBI CYCGRG 1 cut(s) 1040
Bsp119I TTCGAA 1 cut(s) 44
Bsp120I GGGCCC 1 cut(s) 1009
Bsp1286I GDGCHC 2 cut(s) 731, 1013
Bsp1407I TGTACA 1 cut(s) 1098
Bsp143I GATC 9 cut(s) 90, 201, 412, 523, 531, 573, 703, 1156, 1235
BspANI GGCC 5 cut(s) 188, 308, 1011, 1061, 1262
BspCNI CTCAG 2 cut(s) 548, 1067
BspLI GGNNCC 4 cut(s) 26, 113, 351, 1011
BspPI GGATC 4 cut(s) 209, 581, 1164, 1243
BspT104I TTCGAA 1 cut(s) 44
BspTI CTTAAG 1 cut(s) 766
BsrDI GCAATG 1 cut(s) 1080
BsrGI TGTACA 1 cut(s) 1098
BsrI ACTGG 3 cut(s) 25, 373, 557
BssMI GATC 9 cut(s) 90, 201, 412, 523, 531, 573, 703, 1156, 1235
BssNAI GTATAC 1 cut(s) 567
BssSI CACGAG 1 cut(s) 722
Bst1107I GTATAC 1 cut(s) 567
Bst2BI CACGAG 1 cut(s) 722
Bst2UI CCWGG 1 cut(s) 38
Bst4CI ACNGT 4 cut(s) 439, 713, 1119, 1184
Bst6I CTCTTC 5 cut(s) 422, 910, 957, 963, 969
BstAFI CTTAAG 1 cut(s) 766
BstAUI TGTACA 1 cut(s) 1098
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 1 cut(s) 433
BstDEI CTNAG 4 cut(s) 299, 535, 1054, 1210
BstF5I GGATG 4 cut(s) 255, 300, 494, 1237
BstKTI GATC 9 cut(s) 93, 204, 415, 526, 534, 576, 706, 1159, 1238
BstMAI GTCTC 1 cut(s) 1134
BstMBI GATC 9 cut(s) 90, 201, 412, 523, 531, 573, 703, 1156, 1235
BstMWI GCNNNNNNNGC 1 cut(s) 104
BstNI CCWGG 1 cut(s) 38
BstNSI RCATGY 1 cut(s) 1099
BstSCI CCNGG 2 cut(s) 36, 1006
BstSLI GKGCMC 1 cut(s) 1013
BstV1I GCAGC 3 cut(s) 868, 875, 1090
BstV2I GAAGAC 2 cut(s) 1185, 1237
BstX2I RGATCY 3 cut(s) 90, 531, 1156
BstYI RGATCY 3 cut(s) 90, 531, 1156
BstZ17I GTATAC 1 cut(s) 567
BsuRI GGCC 5 cut(s) 188, 308, 1011, 1061, 1262
BtgZI GCGATG 1 cut(s) 717
BtsCI GGATG 4 cut(s) 255, 300, 494, 1237
BtsIMutI CAGTG 2 cut(s) 408, 435
Cac8I GCNNGC 1 cut(s) 433
Cfr13I GGNCC 7 cut(s) 187, 306, 325, 1004, 1009, 1010, 1260
Csp6I GTAC 3 cut(s) 31, 628, 1099
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviAII CATG 7 cut(s) 104, 319, 653, 995, 1096, 1102, 1141
CviQI GTAC 3 cut(s) 31, 628, 1099
DdeI CTNAG 4 cut(s) 299, 535, 1054, 1210
DpnI GATC 9 cut(s) 92, 203, 414, 525, 533, 575, 705, 1158, 1237
DpnII GATC 9 cut(s) 90, 201, 412, 523, 531, 573, 703, 1156, 1235
Eam1104I CTCTTC 5 cut(s) 422, 910, 957, 963, 969
EarI CTCTTC 5 cut(s) 422, 910, 957, 963, 969
Ecl136II GAGCTC 1 cut(s) 729
Eco24I GRGCYC 2 cut(s) 731, 1013
Eco47I GGWCC 2 cut(s) 325, 1004
Eco53kI GAGCTC 1 cut(s) 729
Eco57I CTGAAG 2 cut(s) 489, 681
Eco88I CYCGRG 1 cut(s) 1040
EcoICRI GAGCTC 1 cut(s) 729
EcoO109I RGGNCCY 2 cut(s) 187, 1260
EcoRI GAATTC 2 cut(s) 230, 578
EcoRII CCWGG 1 cut(s) 36
EcoT22I ATGCAT 1 cut(s) 131
EcoT38I GRGCYC 2 cut(s) 731, 1013
FaeI CATG 7 cut(s) 107, 322, 656, 998, 1099, 1105, 1144
FatI CATG 7 cut(s) 103, 318, 652, 994, 1095, 1101, 1140
FauNDI CATATG 1 cut(s) 125
FbaI TGATCA 1 cut(s) 703
FblI GTMKAC 1 cut(s) 566
Fnu4HI GCNGC 3 cut(s) 864, 882, 1079
FokI GGATG 4 cut(s) 262, 307, 481, 1244
FriOI GRGCYC 2 cut(s) 731, 1013
Fsp4HI GCNGC 3 cut(s) 864, 882, 1079
FspBI CTAG 3 cut(s) 108, 198, 668
GluI GCNGC 3 cut(s) 864, 882, 1079
GsaI CCCAGC 1 cut(s) 431
GsuI CTGGAG 1 cut(s) 951
HaeIII GGCC 5 cut(s) 188, 308, 1011, 1061, 1262
HapII CCGG 2 cut(s) 28, 1007
Hin1II CATG 7 cut(s) 107, 322, 656, 998, 1099, 1105, 1144
HincII GTYRAC 1 cut(s) 1093
HindII GTYRAC 1 cut(s) 1093
HindIII AAGCTT 1 cut(s) 784
HpaII CCGG 2 cut(s) 28, 1007
HphI GGTGA 4 cut(s) 71, 631, 844, 1199
Hpy166II GTNNAC 3 cut(s) 567, 1093, 1122
Hpy188I TCNGA 7 cut(s) 508, 538, 606, 661, 1057, 1200, 1271
Hpy8I GTNNAC 3 cut(s) 567, 1093, 1122
HpyAV CCTTC 4 cut(s) 125, 656, 982, 1222
HpyCH4III ACNGT 4 cut(s) 439, 713, 1119, 1184
HpyCH4V TGCA 7 cut(s) 129, 155, 442, 695, 1073, 1078, 1114
HpyF10VI GCNNNNNNNGC 1 cut(s) 104
HpyF3I CTNAG 4 cut(s) 299, 535, 1054, 1210
Hsp92II CATG 7 cut(s) 107, 322, 656, 998, 1099, 1105, 1144
Ksp22I TGATCA 1 cut(s) 703
Kzo9I GATC 9 cut(s) 90, 201, 412, 523, 531, 573, 703, 1156, 1235
LmnI GCTCC 3 cut(s) 860, 1039, 1152
Lsp1109I GCAGC 3 cut(s) 868, 875, 1090
LweI GCATC 4 cut(s) 259, 285, 704, 932
MaeI CTAG 3 cut(s) 108, 198, 668
MaeIII GTNAC 3 cut(s) 364, 619, 850
MalI GATC 9 cut(s) 92, 203, 414, 525, 533, 575, 705, 1158, 1237
MboI GATC 9 cut(s) 90, 201, 412, 523, 531, 573, 703, 1156, 1235
MflI RGATCY 3 cut(s) 90, 531, 1156
MhlI GDGCHC 2 cut(s) 731, 1013
MluCI AATT 6 cut(s) 230, 264, 354, 578, 835, 874
MlyI GAGTC 5 cut(s) 379, 442, 610, 1136, 1251
MmeI TCCRAC 1 cut(s) 923
Mph1103I ATGCAT 1 cut(s) 131
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 1 cut(s) 767
MslI CAYNNNNRTG 2 cut(s) 651, 1100
MspA1I CMGCKG 1 cut(s) 431
MspCI CTTAAG 1 cut(s) 766
MspI CCGG 2 cut(s) 28, 1007
MspR9I CCNGG 2 cut(s) 38, 1008
MvaI CCWGG 1 cut(s) 38
MwoI GCNNNNNNNGC 1 cut(s) 104
NciI CCSGG 1 cut(s) 1008
NdeI CATATG 1 cut(s) 125
NdeII GATC 9 cut(s) 90, 201, 412, 523, 531, 573, 703, 1156, 1235
NlaIII CATG 7 cut(s) 107, 322, 656, 998, 1099, 1105, 1144
NlaIV GGNNCC 4 cut(s) 26, 113, 351, 1011
NmeAIII GCCGAG 1 cut(s) 1087
NmuCI GTSAC 3 cut(s) 364, 619, 850
NsiI ATGCAT 1 cut(s) 131
NspI RCATGY 1 cut(s) 1099
NspV TTCGAA 1 cut(s) 44
PciI ACATGT 1 cut(s) 1095
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 9 cut(s) 5, 41, 82, 223, 339, 607, 656, 818, 1195
PflMI CCANNNNNTGG 1 cut(s) 334
PkrI GCNGC 3 cut(s) 865, 883, 1080
PleI GAGTC 5 cut(s) 379, 442, 609, 1135, 1250
PpsI GAGTC 5 cut(s) 379, 442, 609, 1135, 1250
PscI ACATGT 1 cut(s) 1095
Psp124BI GAGCTC 1 cut(s) 731
Psp6I CCWGG 1 cut(s) 36
PspFI CCCAGC 1 cut(s) 427
PspGI CCWGG 1 cut(s) 36
PspN4I GGNNCC 4 cut(s) 26, 113, 351, 1011
PspOMI GGGCCC 1 cut(s) 1009
PspPI GGNCC 7 cut(s) 187, 306, 325, 1004, 1009, 1010, 1260
PsuI RGATCY 3 cut(s) 90, 531, 1156
PvuII CAGCTG 1 cut(s) 431
RsaI GTAC 3 cut(s) 32, 629, 1100
RsaNI GTAC 3 cut(s) 31, 628, 1099
RseI CAYNNNNRTG 2 cut(s) 651, 1100
SacI GAGCTC 1 cut(s) 731
SaqAI TTAA 1 cut(s) 767
SatI GCNGC 3 cut(s) 864, 882, 1079
Sau3AI GATC 9 cut(s) 90, 201, 412, 523, 531, 573, 703, 1156, 1235
Sau96I GGNCC 7 cut(s) 187, 306, 325, 1004, 1009, 1010, 1260
ScaI AGTACT 1 cut(s) 629
SchI GAGTC 5 cut(s) 379, 442, 610, 1136, 1251
ScrFI CCNGG 2 cut(s) 38, 1008
SduI GDGCHC 2 cut(s) 731, 1013
SfaNI GCATC 4 cut(s) 259, 285, 704, 932
SfuI TTCGAA 1 cut(s) 44
SinI GGWCC 2 cut(s) 325, 1004
SmiMI CAYNNNNRTG 2 cut(s) 651, 1100
SmlI CTYRAG 6 cut(s) 86, 244, 387, 766, 787, 904
SmoI CTYRAG 6 cut(s) 86, 244, 387, 766, 787, 904
SpeI ACTAGT 1 cut(s) 667
Sse9I AATT 6 cut(s) 230, 264, 354, 578, 835, 874
SspMI CTAG 3 cut(s) 108, 198, 668
SstI GAGCTC 1 cut(s) 731
StyD4I CCNGG 2 cut(s) 36, 1006
TaaI ACNGT 4 cut(s) 439, 713, 1119, 1184
TaqI TCGA 4 cut(s) 44, 526, 576, 825
TaqII GACCGA 1 cut(s) 813
TasI AATT 6 cut(s) 230, 264, 354, 578, 835, 874
TatI WGTACW 2 cut(s) 627, 1098
TfiI GAWTC 9 cut(s) 5, 41, 82, 223, 339, 607, 656, 818, 1195
Tru1I TTAA 1 cut(s) 767
Tru9I TTAA 1 cut(s) 767
TscAI CASTG 2 cut(s) 415, 442
TseFI GTSAC 3 cut(s) 364, 619, 850
TseI GCWGC 3 cut(s) 863, 881, 1078
Tsp45I GTSAC 3 cut(s) 364, 619, 850
TspDTI ATGAA 7 cut(s) 150, 252, 951, 998, 1011, 1064, 1157
TspGWI ACGGA 1 cut(s) 638
TspRI CASTG 2 cut(s) 415, 442
Van91I CCANNNNNTGG 1 cut(s) 334
Vha464I CTTAAG 1 cut(s) 766
VpaK11BI GGWCC 2 cut(s) 325, 1004
XapI RAATTY 2 cut(s) 230, 578
XceI RCATGY 1 cut(s) 1099
XcmI CCANNNNNNNNNTGG 2 cut(s) 316, 428
XmiI GTMKAC 1 cut(s) 566
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 3 cut(s) 108, 198, 668
ZrmI AGTACT 1 cut(s) 629
Zsp2I ATGCAT 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.