MD03G1166500.v1.1

Protein kinase C conserved region 2 (CalB)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0012150
Physical Location & Seq
Reverse (-)
22242844 .. 22244211
1368 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1166500.v1.1.491

Sequence Viewer

Length: 1368 bp
ATGGAATCATTCAATCCAACAGCCGGATTCCGGTACAACCTGAATTCAAACACAATAGCCGATGAAAATAGCGTTTCCGATCAGTTTTCAGGGATTCTTGAAATCTATGCTCATCATGCTAGGAACATTCACAACATATGCATCTATGAAAACCAAGATGTGTACGCCAAATTCTCTCTTACGTACAATCCAGACGAGACGCTCACCACTAGGGTCATCAATGGAGGTGGCAAGAACCCCGAATTCAACGAAAGCTTGAGGATCAAAATTGGTCAACTGGATGCAGTCCTCAAATGCGAGATTTGGATGCTGAGCAGGGCAAGAAACTACATGGAAGACCAGCTGTTGGGATTCACTTTGGTCCCAATTTCTCAAGTGACTGGGAAGGGAAAGGTCACTCAGGATTATAGTCTCTCTTCCACTGATCTCTTCCATTCCCCTGCTGGAACTGTCAAACTAACTCTTTCTCTCCATACTTCTTTGCCTGTTAAACCCTCCACTAGTTCGTTGTCGGAATCATCCGCCAACTCTTCCATAACCTCCGAAGTTGTATTGCTTGATCGAAAAATATCTCAAGTTGCGTTAGACCCGGTTGAGTATTCGAGGATGGAATTCCCAGACATTGATGTGGCGAGTAAGAATCAACAAATGGTGACCGAGTATTTTAGTTTGGCAAGGCGTGACTGTGCTTGGAGACCTGGTCCCGAAGGTCTTGGATCGTTTCTTCATCTCGGTGCATCTCCTCAGCCTGCTGCTGATCCTGACTATGAAATGACTGTAAGTTCATGTGAGGGGATTCAGGGTGACCCTGTTTCTCCCAATGGGAGCATCCAGAACTCCGGCTTCTTAAGTTGTACCACCACTAGCTTGAGCGATGATCGAAACTCCGCTGATTCAATCGACAAGAAGAATCTTGTGGCTGGTGAATCGTCGAATTCTCTCAATGCTTCTGTCACCGCCGGAGCAGCTAATCAAGGTTCCGCTGCTTGTCCAGACACCCCAACCTCAAGAAAGGGCAGAGAAGGCAGAGACGAAAAGGAGTCTAACTTGTCAGGCAAGGAAGAGGAGAGCAACAAGGAACAGAAAGTTGGATCTGGTCAATTTGGTCAAGTATTTTCTGCTCCACTTGGGAATATAAACATGGAGGCGGAGCAATCTGCAATGCAGCAACAAATAGTGGACATGTACATGAGAAGCATGCAGCAATTCACCGAGTCTTTGGCGAAGATGAAGCTCCCGATGGATCTTGATAAAGCAGAATGTGAAGACCGCAGCGAAGTGATTCCAAACCATAGCAACAAGAGTTTAGAAGTTGATAAAAAAAAGGACGGATCGCGGGTGTTCTATGGCAGCCGTGCCTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

456

Amino Acids

49.8

Weight (kDa)

4.96

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 31 - 137 2.2e-08 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012150)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G12300
fragaria_vesca FvH4_3g27890 FvH4_3g27890 FvH4_3g27890
malus_domestica MD03G1166500.v1.1
prunus_persica Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1 Prupe.4G267000_v2.0.a1
pyrus_communis pycom03g12210
rosa_chinensis RchiOBHm_Chr5g0050621
rosa_laevigata RLG00000034712
rosa_multiflora Rmu_ssc0000350.1_g000030
rosa_roxburghii Rroxscaffold_1G00030180
rosa_rugosa Rorug05G0262100
rosa_samantha Rh5AG334400 Rh5BG343800 Rh5CG371600 Rh5DG356800
rosa_wichuraiana Rw5G031550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 346
AccII CGCG 1 cut(s) 1336
AciI CCGC 7 cut(s) 522, 888, 957, 981, 1148, 1270, 1336
AclWI GGATC 6 cut(s) 269, 724, 752, 1099, 1251, 1339
AcsI RAATTY 5 cut(s) 43, 170, 242, 611, 934
AfaI GTAC 5 cut(s) 35, 164, 185, 856, 1187
AfiI CCNNNNNNNGG 3 cut(s) 23, 30, 346
AflII CTTAAG 1 cut(s) 847
AflIII ACRYGT 1 cut(s) 1182
AgsI TTSAA 5 cut(s) 13, 48, 101, 247, 897
AhlI ACTAGT 1 cut(s) 500
AjnI CCWGG 1 cut(s) 697
AjuI GAANNNNNNNTTGG 2 cut(s) 1071, 1103
AluBI AGCT 5 cut(s) 255, 343, 867, 968, 1234
AluI AGCT 5 cut(s) 255, 343, 867, 968, 1234
Alw26I GTCTC 4 cut(s) 191, 416, 688, 1023
AlwI GGATC 6 cut(s) 269, 724, 752, 1099, 1251, 1339
ApeKI GCWGC 7 cut(s) 752, 965, 983, 1165, 1201, 1272, 1350
ApoI RAATTY 5 cut(s) 43, 170, 242, 611, 934
Asp700I GAANNNNTTC 1 cut(s) 1281
AspS9I GGNCC 2 cut(s) 361, 701
AsuC2I CCSGG 1 cut(s) 590
AsuHPI GGTGA 6 cut(s) 196, 664, 815, 935, 946, 1201
AvaII GGWCC 2 cut(s) 361, 701
BbsI GAAGAC 2 cut(s) 342, 1272
BbvCI CCTCAGC 1 cut(s) 744
BbvI GCAGC 7 cut(s) 739, 970, 977, 1177, 1213, 1284, 1362
BccI CCATC 2 cut(s) 601, 1234
BceAI ACGGC 1 cut(s) 1338
BciT130I CCWGG 1 cut(s) 699
BcnI CCSGG 1 cut(s) 590
BcoDI GTCTC 4 cut(s) 191, 416, 688, 1023
BcuI ACTAGT 1 cut(s) 500
BfaI CTAG 4 cut(s) 120, 210, 501, 864
BfrI CTTAAG 1 cut(s) 847
BisI GCNGC 7 cut(s) 753, 966, 984, 1166, 1202, 1273, 1351
BlpI GCTNAGC 1 cut(s) 311
BlsI GCNGC 7 cut(s) 754, 967, 985, 1167, 1203, 1274, 1352
Bme1390I CCNGG 2 cut(s) 590, 699
Bme18I GGWCC 2 cut(s) 361, 701
BmgT120I GGNCC 2 cut(s) 361, 701
BmiI GGNNCC 3 cut(s) 363, 703, 979
BmrFI CCNGG 2 cut(s) 590, 699
BmrI ACTGGG 1 cut(s) 390
BmsI GCATC 5 cut(s) 150, 271, 297, 746, 837
BmuI ACTGGG 1 cut(s) 390
BpiI GAAGAC 2 cut(s) 342, 1272
Bpu10I CCTNAGC 1 cut(s) 744
Bpu1102I GCTNAGC 1 cut(s) 311
BpuEI CTTGAG 5 cut(s) 277, 357, 558, 889, 991
BpuMI CCSGG 1 cut(s) 590
BsaAI YACGTR 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 688
BsaWI WCCGGW 1 cut(s) 30
BsaXI ACNNNNNCTCC 2 cut(s) 685, 715
Bsc4I CCNNNNNNNGG 3 cut(s) 23, 30, 346
Bse1I ACTGG 2 cut(s) 282, 385
Bse3DI GCAATG 1 cut(s) 1167
BseBI CCWGG 1 cut(s) 699
BseGI GGATG 5 cut(s) 286, 312, 518, 612, 828
BseLI CCNNNNNNNGG 3 cut(s) 23, 30, 346
BseMI GCAATG 1 cut(s) 1167
BseMII CTCAG 3 cut(s) 302, 413, 758
BseNI ACTGG 2 cut(s) 282, 385
BseRI GAGGAG 2 cut(s) 732, 1079
BseXI GCAGC 7 cut(s) 739, 970, 977, 1177, 1213, 1284, 1362
Bsh1236I CGCG 1 cut(s) 1336
BsiSI CCGG 5 cut(s) 24, 31, 590, 840, 960
BslFI GGGAC 2 cut(s) 347, 687
BslI CCNNNNNNNGG 3 cut(s) 23, 30, 346
BsmAI GTCTC 4 cut(s) 191, 416, 688, 1023
BsmBI CGTCTC 2 cut(s) 191, 1023
BsmFI GGGAC 2 cut(s) 347, 687
Bso31I GGTCTC 1 cut(s) 688
Bsp1407I TGTACA 1 cut(s) 1185
Bsp1720I GCTNAGC 1 cut(s) 311
BspACI CCGC 7 cut(s) 522, 888, 957, 981, 1148, 1270, 1336
BspCNI CTCAG 3 cut(s) 303, 412, 757
BspFNI CGCG 1 cut(s) 1336
BspLI GGNNCC 3 cut(s) 363, 703, 979
BspPI GGATC 6 cut(s) 269, 724, 752, 1099, 1251, 1339
BspTI CTTAAG 1 cut(s) 847
BspTNI GGTCTC 1 cut(s) 688
BsrDI GCAATG 1 cut(s) 1167
BsrGI TGTACA 1 cut(s) 1185
BsrI ACTGG 2 cut(s) 282, 385
Bst2UI CCWGG 1 cut(s) 699
Bst4CI ACNGT 3 cut(s) 451, 686, 778
Bst6I CTCTTC 4 cut(s) 421, 434, 535, 1056
BstAFI CTTAAG 1 cut(s) 847
BstAUI TGTACA 1 cut(s) 1185
BstBAI YACGTR 1 cut(s) 183
BstC8I GCNNGC 2 cut(s) 750, 1199
BstDEI CTNAG 3 cut(s) 311, 399, 744
BstEII GGTNACC 2 cut(s) 652, 803
BstF5I GGATG 5 cut(s) 286, 312, 518, 612, 828
BstFNI CGCG 1 cut(s) 1336
BstMAI GTCTC 4 cut(s) 191, 416, 688, 1023
BstMWI GCNNNNNNNGC 3 cut(s) 116, 965, 1023
BstNI CCWGG 1 cut(s) 699
BstNSI RCATGY 2 cut(s) 1186, 1201
BstPI GGTNACC 2 cut(s) 652, 803
BstSCI CCNGG 2 cut(s) 588, 697
BstSNI TACGTA 1 cut(s) 183
BstUI CGCG 1 cut(s) 1336
BstV1I GCAGC 7 cut(s) 739, 970, 977, 1177, 1213, 1284, 1362
BstV2I GAAGAC 2 cut(s) 342, 1272
BstX2I RGATCY 2 cut(s) 1091, 1243
BstYI RGATCY 2 cut(s) 1091, 1243
BtgZI GCGATG 1 cut(s) 888
BtsCI GGATG 5 cut(s) 286, 312, 518, 612, 828
BtsIMutI CAGTG 1 cut(s) 420
Cac8I GCNNGC 2 cut(s) 750, 1199
Cfr13I GGNCC 2 cut(s) 361, 701
CseI GACGC 1 cut(s) 208
CsiI ACCWGGT 1 cut(s) 697
Csp6I GTAC 5 cut(s) 34, 163, 184, 855, 1186
CspCI CAANNNNNGTGG 2 cut(s) 208, 243
CviAII CATG 7 cut(s) 116, 331, 786, 1141, 1183, 1189, 1198
CviQI GTAC 5 cut(s) 34, 163, 184, 855, 1186
DdeI CTNAG 3 cut(s) 311, 399, 744
Eam1104I CTCTTC 4 cut(s) 421, 434, 535, 1056
EarI CTCTTC 4 cut(s) 421, 434, 535, 1056
EciI GGCGGA 2 cut(s) 511, 1163
Eco105I TACGTA 1 cut(s) 183
Eco31I GGTCTC 1 cut(s) 688
Eco47I GGWCC 2 cut(s) 361, 701
Eco91I GGTNACC 2 cut(s) 652, 803
EcoO65I GGTNACC 2 cut(s) 652, 803
EcoRI GAATTC 4 cut(s) 43, 242, 611, 934
EcoRII CCWGG 1 cut(s) 697
EcoT22I ATGCAT 1 cut(s) 143
Esp3I CGTCTC 2 cut(s) 191, 1023
FaeI CATG 7 cut(s) 119, 334, 789, 1144, 1186, 1192, 1201
FaqI GGGAC 2 cut(s) 347, 687
FatI CATG 7 cut(s) 115, 330, 785, 1140, 1182, 1188, 1197
FauI CCCGC 1 cut(s) 1329
FauNDI CATATG 1 cut(s) 137
Fnu4HI GCNGC 7 cut(s) 753, 966, 984, 1166, 1202, 1273, 1351
FokI GGATG 5 cut(s) 293, 319, 505, 619, 815
Fsp4HI GCNGC 7 cut(s) 753, 966, 984, 1166, 1202, 1273, 1351
FspBI CTAG 4 cut(s) 120, 210, 501, 864
GluI GCNGC 7 cut(s) 753, 966, 984, 1166, 1202, 1273, 1351
HapII CCGG 5 cut(s) 24, 31, 590, 840, 960
HgaI GACGC 1 cut(s) 208
Hin1II CATG 7 cut(s) 119, 334, 789, 1144, 1186, 1192, 1201
HincII GTYRAC 1 cut(s) 275
HindII GTYRAC 1 cut(s) 275
HindIII AAGCTT 1 cut(s) 253
HpaII CCGG 5 cut(s) 24, 31, 590, 840, 960
HphI GGTGA 6 cut(s) 196, 664, 815, 935, 946, 1201
Hpy166II GTNNAC 3 cut(s) 163, 275, 1180
Hpy188I TCNGA 4 cut(s) 79, 514, 544, 1367
Hpy8I GTNNAC 3 cut(s) 163, 275, 1180
Hpy99I CGWCG 1 cut(s) 934
HpyAV CCTTC 3 cut(s) 379, 701, 1016
HpyCH4III ACNGT 3 cut(s) 451, 686, 778
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 6 cut(s) 141, 284, 737, 1160, 1165, 1201
HpyF10VI GCNNNNNNNGC 3 cut(s) 116, 965, 1023
HpyF3I CTNAG 3 cut(s) 311, 399, 744
HpySE526I ACGT 1 cut(s) 182
Hsp92II CATG 7 cut(s) 119, 334, 789, 1144, 1186, 1192, 1201
LmnI GCTCC 5 cut(s) 825, 962, 1126, 1150, 1239
Lsp1109I GCAGC 7 cut(s) 739, 970, 977, 1177, 1213, 1284, 1362
LweI GCATC 5 cut(s) 150, 271, 297, 746, 837
MabI ACCWGGT 1 cut(s) 697
MaeI CTAG 4 cut(s) 120, 210, 501, 864
MaeII ACGT 1 cut(s) 182
MaeIII GTNAC 6 cut(s) 376, 394, 652, 680, 803, 952
MflI RGATCY 2 cut(s) 1091, 1243
MluCI AATT 9 cut(s) 43, 170, 242, 267, 366, 611, 934, 1100, 1205
MlyI GAGTC 2 cut(s) 1049, 1223
MmeI TCCRAC 3 cut(s) 41, 492, 1069
Mph1103I ATGCAT 1 cut(s) 143
MroXI GAANNNNTTC 1 cut(s) 1281
MseI TTAA 2 cut(s) 489, 848
MslI CAYNNNNRTG 3 cut(s) 626, 732, 1187
MspA1I CMGCKG 3 cut(s) 343, 890, 983
MspCI CTTAAG 1 cut(s) 847
MspI CCGG 5 cut(s) 24, 31, 590, 840, 960
MspR9I CCNGG 2 cut(s) 590, 699
MvaI CCWGG 1 cut(s) 699
MvnI CGCG 1 cut(s) 1336
MwoI GCNNNNNNNGC 3 cut(s) 116, 965, 1023
NciI CCSGG 1 cut(s) 590
NdeI CATATG 1 cut(s) 137
NlaIII CATG 7 cut(s) 119, 334, 789, 1144, 1186, 1192, 1201
NlaIV GGNNCC 3 cut(s) 363, 703, 979
NmuCI GTSAC 6 cut(s) 376, 394, 652, 680, 803, 952
NsiI ATGCAT 1 cut(s) 143
NspI RCATGY 2 cut(s) 1186, 1201
PaeI GCATGC 1 cut(s) 1201
PciI ACATGT 1 cut(s) 1182
PdmI GAANNNNTTC 1 cut(s) 1281
PflFI GACNNNGTC 1 cut(s) 699
PflMI CCANNNNNTGG 1 cut(s) 346
PkrI GCNGC 7 cut(s) 754, 967, 985, 1167, 1203, 1274, 1352
PleI GAGTC 2 cut(s) 1048, 1222
PpsI GAGTC 2 cut(s) 1048, 1222
Ppu21I YACGTR 1 cut(s) 183
PscI ACATGT 1 cut(s) 1182
Psp6I CCWGG 1 cut(s) 697
PspEI GGTNACC 2 cut(s) 652, 803
PspGI CCWGG 1 cut(s) 697
PspN4I GGNNCC 3 cut(s) 363, 703, 979
PspPI GGNCC 2 cut(s) 361, 701
PsuI RGATCY 2 cut(s) 1091, 1243
PsyI GACNNNGTC 1 cut(s) 699
PvuII CAGCTG 1 cut(s) 343
RsaI GTAC 5 cut(s) 35, 164, 185, 856, 1187
RsaNI GTAC 5 cut(s) 34, 163, 184, 855, 1186
RseI CAYNNNNRTG 3 cut(s) 626, 732, 1187
SaqAI TTAA 2 cut(s) 489, 848
SatI GCNGC 7 cut(s) 753, 966, 984, 1166, 1202, 1273, 1351
Sau96I GGNCC 2 cut(s) 361, 701
SchI GAGTC 2 cut(s) 1049, 1223
ScrFI CCNGG 2 cut(s) 590, 699
SexAI ACCWGGT 1 cut(s) 697
SfaNI GCATC 5 cut(s) 150, 271, 297, 746, 837
SinI GGWCC 2 cut(s) 361, 701
SmiMI CAYNNNNRTG 3 cut(s) 626, 732, 1187
SmlI CTYRAG 6 cut(s) 256, 372, 573, 847, 868, 1006
SmoI CTYRAG 6 cut(s) 256, 372, 573, 847, 868, 1006
SnaBI TACGTA 1 cut(s) 183
SpeI ACTAGT 1 cut(s) 500
SphI GCATGC 1 cut(s) 1201
Sse9I AATT 9 cut(s) 43, 170, 242, 267, 366, 611, 934, 1100, 1205
SsiI CCGC 7 cut(s) 522, 888, 957, 981, 1148, 1270, 1336
SspMI CTAG 4 cut(s) 120, 210, 501, 864
StyD4I CCNGG 2 cut(s) 588, 697
TaaI ACNGT 3 cut(s) 451, 686, 778
TaiI ACGT 1 cut(s) 185
TaqI TCGA 5 cut(s) 562, 602, 880, 900, 932
TaqII GACCGA 1 cut(s) 671
TasI AATT 9 cut(s) 43, 170, 242, 267, 366, 611, 934, 1100, 1205
TatI WGTACW 1 cut(s) 1185
Tru1I TTAA 2 cut(s) 489, 848
Tru9I TTAA 2 cut(s) 489, 848
TscAI CASTG 1 cut(s) 427
TseFI GTSAC 6 cut(s) 376, 394, 652, 680, 803, 952
TseI GCWGC 7 cut(s) 752, 965, 983, 1165, 1201, 1272, 1350
Tsp45I GTSAC 6 cut(s) 376, 394, 652, 680, 803, 952
TspDTI ATGAA 6 cut(s) 78, 162, 716, 774, 783, 1244
TspGWI ACGGA 1 cut(s) 1344
TspRI CASTG 1 cut(s) 427
Tth111I GACNNNGTC 1 cut(s) 699
Van91I CCANNNNNTGG 1 cut(s) 346
Vha464I CTTAAG 1 cut(s) 847
VpaK11BI GGWCC 2 cut(s) 361, 701
XapI RAATTY 5 cut(s) 43, 170, 242, 611, 934
XceI RCATGY 2 cut(s) 1186, 1201
XcmI CCANNNNNNNNNTGG 1 cut(s) 440
XmnI GAANNNNTTC 1 cut(s) 1281
XspI CTAG 4 cut(s) 120, 210, 501, 864
Zsp2I ATGCAT 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.