AT5G13530

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010072
Physical Location & Seq
Forward (+)
4345202 .. 4354723
9522 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G13530.1

Sequence Viewer

Length: 4878 bp
ATGGTTGGGAGAGTCAAGGTCCCTTGTTGTTCCGTGTGCCACACGCGGTATAATGAAGATGAGAGAGTTCCTTTATTGCTTCAATGCGGCCATGGCTTCTGCAAAGATTGTCTTTCCAAGATGTTCTCTACTTCTTCCGACACAACCCTCACTTGTCCCCGGTGTCGCCACGTGTCTGTTGTTGGGAACTCTGTTCAGGGTCTCAGGAAGAACTACGCTATGCTTGCTCTCATTCACGCCGCATCCGGCGGCGCTAACTTCGATTGCGATTACACGGATGATGAGGATGACGACGATGAAGAAGATGGTAGTGATGAAGATGGAGCTCGTGCCGCTCGTGGATTTCATGCCTCAAGCTCGATTAATAGCTTGTGTGGTCCAGTGATCGAGGTCGGAGCTCACCCGGAGATGAAGCTGGTGAGGCAGATTGGGGAAGAGAGTAGTAGTGGTGGTTTTGGAGGTGTGGAGATGTGGGATGCAACTGTTGCAGGCGGTGGTGGACGGTGTAAGCACCGTGTGGCGGTGAAAAAGATGACTTTGACGGAGGACATGGATGTTGAGTGGATGCAGGGACAGCTTGAGAGCTTGCGGAGAGCATCCATGTGGTGTAGGAATGTTTGTACTTTCCATGGGGTTGTGAAGATGGATGGATCTCTTTGTCTTTTGATGGACAGGTGCTTTGGGTCTGTTCAGTCTGAGATGCAACGCAATGAAGGCAGACTTACTTTGGAGCAGATTCTAAGATATGGGGCTGATGTTGCTCGAGGGGTTGCAGAACTCCATGCAGCAGGTGTTATCTGTATGAATATAAAACCTTCCAATCTTCTTTTGGATGCAAGCGGCAATGCTGTGGTTTCTGATTATGGGCTCGCTCCCATCCTAAAGAAGCCAACATGCCAGAAAACTCGACCAGAGTTTGATTCCTCAAAAGTCACTCTATACACGGACTGTGTAACACTCAGTCCACACTACACTGCCCCAGAGGCTTGGGGACCGGTGAAAAAACTTTTTTGGGAGGATGCAAGTGGTGTATCTCCAGAATCAGATGCATGGAGTTTTGGCTGTACGTTAGTAGAGATGTGCACTGGATCCACTCCGTGGGATGGTCTTAGTAGAGAAGAAATTTTTCAAGCTGTTGTGAAAGCCCGAAAAGTACCACCACAGTATGAACGCATAGTGGGTGTGGGAATTCCTCGGGAATTATGGAAAATGATTGGTGAATGTCTTCAGTTTAAACCGTCGAAAAGACCAACCTTTAATGCAATGCTTGCTACATTTCTTCGTCATCTACAAGAGATACCACGTAGCCCTTCAGCAAGCCCTGATAATGGGATTGCCAAAATATGTGAAGTAAACATTGTACAAGCACCTCGCGCGACTAACATAGGGGTCTTTCAGGATAACCCTAACAACCTACATCGGGTTGTTTTGGAGGGAGACTTTGAGGGTGTAAGAAATATACTTGCAAAGGCTGCAGCTGGAGGCGGTGGAAGCTCTGTGCGATCTCTATTAGAGGCGCAAAACGCTGATGGTCAATCGGCTCTTCACTTAGCTTGCAGACGTGGCAGTGCTGAACTTGTGGAGGCTATACTGGAGTATGGGGAGGCTAATGTAGATATCGTGGACAAAGATGGGGATCCTCCTCTTGTCTTTGCTTTAGCAGCTGGATCTCCACAATGTGTTCACGTTCTCATTAAAAAGGGGGCTAATGTGAGGTCCAGACTGCGGGAAGGATCTGGCCCATCTGTAGCTCACGTGTGTTCGTACCATGGCCAACCTGATTGTATGCGTGAATTGCTGGTAGCTGGAGCCGATCCAAATGCAGTGGATGATGAAGGTGAAACTGTGCTTCATCGAGCTGTTGCCAAGAAATACACAGATTGTGCCATTGTTATATTGGAAAATGGGGGATCTAGATCAATGACTGTTTCAAATGCGAAGTGCCTGACACCGTTACATATGTGTGTAGCAACATGGAATGTTGCTGTTATCAAGAGATGGGTGGAAGTTTCCAGTCCAGAAGAAATTTCTCAGGCAATTAATATCCCAAGCCCAGTTGGCACTGCTCTGTGTATGGCTGCTTCTATAAGGAAAGATCATGAAAAGGAAGGTAGAGAGCTGGTTCAGATATTGCTTGCAGCTGGGGCAGATCCGACTGCGCAAGATGCTCAGCACGGGCGAACAGCGTTGCATACTGCTGCTATGGCTAACAATGTGGAACTTGTGAGAGTCATTCTAGATGCTGGTGTGAATGCGAACATCCGCAATGTTCACAATACAATTCCTCTTCATATGGCTTTGGCCAGAGGCGCAAACTCCTGTGTTAGCCTACTTCTGGAGTCTGGTTCAGACTGCAATATACAGGATGATGAAGGAGATAACGCATTTCATATAGCAGCAGATGCAGCGAAGATGATACGTGAAAACCTTGATTGGTTAATTGTCATGCTTAGAAGTCCTGATGCTGCTGTGGACGTCAGAAACCACAGTGGCAAGACAGTACGTGACTTCTTAGAAGCCCTTCCAAGAGAATGGATATCAGAGGATCTGATGGAGGCACTATTAAAAAGGGGAGTTCATCTATCACCTACAATCTATGAGGTAGGGGATTGGGTGAAATTCAAGAGAGGCATAACAACACCCTTACATGGATGGCAAGGTGCTAAGCCTAAGAGTGTTGGGTTTGTGCAAACCATTCTCGAGAAGGAGGACATGATTATCGCATTTTGTTCTGGTGAAGCTCGTGTGTTGGCAAATGAAGTTGTCAAGTTGATTCCATTAGACAGGGGCCAGCATGTAAGACTCAGAGCAGATGTGAAAGAGCCAAGGTTTGGTTGGCGTGGTCAATCACGTGATAGTGTCGGTACTGTTTTATGTGTTGATGAGGATGGGATCTTACGAGTTGGGTTTCCTGGAGCATCTCGAGGCTGGAAAGCTGATCCTGCTGAAATGGAACGTGTGGAAGAATTCAAAGTCGGGGATTGGGTCCGTATCCGTCAGAATCTGACTTCAGCCAAACATGGTTTTGGATCTGTTGTCCCCGGCAGTATGGGGATTGTTTATTGTGTACGGCCAGATAGCAGTCTCCTTGTGGAGTTAAGTTATCTTCCAAATCCCTGGCATTGTGAACCCGAGGAAGTTGAACCTGTTGCTCCCTTTAGGATTGGTGATCGGGTGTGTGTAAAGCGGTCAGTTGCCGAACCTCGATATGCTTGGGGTGGGGAGACCCATCACAGTGTTGGAAAAATTAGTGAAATAGAGAATGATGGTCTTCTTATTATTGAAATACCAAACCGACCTATACCGTGGCAGGCAGATCCTTCAGACATGGAAAAGATTGATGATTTCAAGGTTGGTGACTGGGTCAGAGTGAAGGCGTCAGTGTCTTCCCCGAAATATGGATGGGAAGACATTACTCGAAATAGTATTGGCGTTATGCACAGCTTAGATGAAGATGGTGATGTGGGTATAGCTTTCTGTTTCAGGAGTAAACCTTTTTCATGTTCTGTGACAGATGTTGAAAAGGTTACGCCCTTTCATGTGGGACAAGAGATTCATATGACACCATCAATCACCCAACCACGACTTGGATGGTCAAATGAGACTCCAGCAACTATTGGTAAAGTAATGAGAATCGACATGGACGGGACTCTGAGTGCACAGGTGACTGGTAGACAGACGTTGTGGAGGGTCTCACCTGGAGATGCAGAGCTGCTTTCAGGTTTTGAAGTTGGTGATTGGGTGCGCTCAAAACCGAGCCTGGGAAACAGACCAAGCTATGATTGGTCTAATGTAGGAAGGGAGAGTATTGCTGTCGTTCATAGCATACAGGAAACAGGTTACTTAGAATTGGCTTGTTGTTTCCGGAAAGGAAGATGGAGTACTCATTACACAGATTTAGAGAAGATTCCAGCTCTCAAAGTGGGCCAGTTTGTGCATTTCCAGAAGGGAATAACAGAACCAAGATGGGGCTGGAGAGCAGCCAAGCCCGATTCACGAGGCATCATAACCACAGTTCATGCTGATGGAGAGGTAAGGGTTGCTTTCTTTGGATTGCCTGGGTTGTGGAGAGGAGATCCAGCAGACTTGGAAGTGGAGCCTATGTTTGAGGTTGGAGAATGGGTAAGACTTAGAGAAGGTGTTTCTTGTTGGAAATCTGTTGGACCAGGAAGTGTTGGTGTAGTGCATGGAGTAGGATATGAGGGAGACGAGTGGGATGGAACCACTTCCGTTTCATTTTGTGGCGAGCAAGAAAGATGGGCGGGGCCCACTTCACACCTAGAGAAAGCAAAGAAGCTTGTGGTTGGACAAAAAACTCGGGTCAAACTGGCAGTGAAACAGCCTAGGTTTGGGTGGTCAGGTCATAGCCATGGAAGTGTAGGAACAATATCAGCCATTGATGCGGATGGTAAGCTACGGATATACACACCAGCCGGCTCTAAAACGTGGATGCTTGACCCATCAGAAGTTGAAACCATAGAAGAAGAAGAGCTCAAGATTGGGGACTGGGTTAGGGTAAAGGCGAGCATCACAACACCCACTTACCAGTGGGGAGAGGTCAATCCTTCAAGCACAGGGGTGGTTCACAGAATGGAGGATGGAGATTTATGTGTATCTTTCTGTTTCCTAGACAGGCTATGGCTATGTAAAGCCGGGGAACTGGAACGCATAAGACCATTTAGAATTGGAGACCGGGTTAAGATAAAGGATGGACTGGTTACACCTCGGTGGGGTTGGGGAATGGAGACCCATGCAAGTAAAGGACATGTTGTGGGAGTGGATGCAAATGGGAAATTGAGAATAAAATTCCTGTGGAGGGAAGGGCGGCCCTGGATTGGTGATCCTGCGGATATAGTTCTAGATGAAACTTCTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Pkinase zf-C3HC4 zf-RING_2 zf-RING_5 zf-RING_UBOX

Protein Analysis

1625

Amino Acids

178.21

Weight (kDa)

5.83

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 9 - 56 1.1e-06 Ring finger domain
zf-C3HC4 PF00097 10 - 55 7.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 10 - 57 4.1e-06 zinc-RING finger domain
zf-RING_UBOX PF13445 10 - 53 6.6e-08 RING-type zinc-finger
PK_Tyr_Ser-Thr PF07714 146 - 424 2.1e-27 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 171 - 424 8.8e-31 Protein kinase domain
Ank_2 PF12796 490 - 573 8.2e-13 Ankyrin repeats (3 copies)
Ank PF00023 510 - 543 1.5e-06 Ankyrin repeat
Ank_2 PF12796 585 - 660 6.2e-08 Ankyrin repeats (3 copies)
Ank_4 PF13637 585 - 630 8.6e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 704 - 789 2.8e-15 Ankyrin repeats (3 copies)
Ank_5 PF13857 724 - 766 2.7e-06 Ankyrin repeats (many copies)
Ank PF00023 725 - 756 7.8e-06 Ankyrin repeat
Ank_4 PF13637 730 - 779 3.4e-06 Ankyrin repeats (many copies)
Ank_4 PF13637 763 - 800 9.7e-06 Ankyrin repeats (many copies)
SH3_15 PF18346 866 - 984 2.3e-18 Mind bomb SH3 repeat domain
SH3_15 PF18346 990 - 1047 9.4e-06 Mind bomb SH3 repeat domain
SH3_15 PF18346 1053 - 1110 1.3e-08 Mind bomb SH3 repeat domain
SH3_15 PF18346 1172 - 1234 3.8e-07 Mind bomb SH3 repeat domain
SH3_15 PF18346 1242 - 1363 3.6e-15 Mind bomb SH3 repeat domain
SH3_15 PF18346 1369 - 1490 8.3e-19 Mind bomb SH3 repeat domain
SH3_15 PF18346 1499 - 1617 2.2e-14 Mind bomb SH3 repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 779
AatII GACGTC 1 cut(s) 2478
Acc16I TGCGCA 1 cut(s) 2160
Acc36I ACCTGC 1 cut(s) 779
AccB7I CCANNNNNTGG 3 cut(s) 1098, 2831, 3587
AccBSI CCGCTC 1 cut(s) 335
AccI GTMKAC 1 cut(s) 3673
AccII CGCG 3 cut(s) 46, 1374, 1376
AccIII TCCGGA 1 cut(s) 3864
AcoI YGGCCR 4 cut(s) 88, 1771, 2301, 3071
AcsI RAATTY 6 cut(s) 1122, 1188, 2025, 2618, 2966, 4805
AcuI CTGAAG 4 cut(s) 1211, 1296, 2994, 3306
AcvI CACGTG 3 cut(s) 172, 1756, 2852
AcyI GRCGYC 2 cut(s) 2475, 3377
AdeI CACNNNGTG 3 cut(s) 517, 1098, 1679
AfaI GTAC 9 cut(s) 622, 1066, 1155, 1362, 1766, 2502, 2866, 3069, 3883
AflIII ACRYGT 4 cut(s) 171, 1755, 2956, 4765
AgeI ACCGGT 1 cut(s) 994
AjiI CACGTC 1 cut(s) 1562
AjnI CCWGG 7 cut(s) 2911, 3116, 3697, 3759, 4057, 4165, 4829
AleI CACNNNNGTG 2 cut(s) 4577, 4726
Alw21I GWGCWC 5 cut(s) 328, 400, 1085, 3661, 4494
Alw26I GTCTC 9 cut(s) 206, 1431, 3089, 3218, 3596, 3697, 4200, 4683, 4739
Alw44I GTGCAC 2 cut(s) 1081, 3657
AlwNI CAGNNNCTG 1 cut(s) 3004
Ama87I CYCGRG 6 cut(s) 762, 1194, 2699, 2922, 3131, 4317
Aor13HI TCCGGA 1 cut(s) 3864
AoxI GGCC 9 cut(s) 88, 1738, 1771, 2301, 2788, 3071, 3925, 4265, 4826
ApaI GGGCCC 1 cut(s) 4269
ApaLI GTGCAC 2 cut(s) 1081, 3657
ApoI RAATTY 6 cut(s) 1122, 1188, 2025, 2618, 2966, 4805
ArsI GACNNNNNNTTYG 2 cut(s) 662, 694
AseI ATTAAT 2 cut(s) 363, 2040
AsiGI ACCGGT 1 cut(s) 994
Asp700I GAANNNNTTC 4 cut(s) 1125, 1224, 2520, 4225
AspA2I CCTAGG 1 cut(s) 4343
AspLEI GCGC 6 cut(s) 254, 1376, 1519, 2161, 2312, 3747
AsuC2I CCSGG 5 cut(s) 160, 404, 3042, 4654, 4694
AvaI CYCGRG 6 cut(s) 762, 1194, 2699, 2922, 3131, 4317
AvaII GGWCC 6 cut(s) 19, 377, 992, 1716, 2986, 4163
AvrII CCTAGG 1 cut(s) 4343
BaeGI GKGCMC 3 cut(s) 1085, 3661, 4269
BalI TGGCCA 2 cut(s) 1773, 2303
BamHI GGATCC 2 cut(s) 1088, 1636
BanII GRGCYC 5 cut(s) 328, 400, 870, 4269, 4494
BarI GAAGNNNNNNTAC 4 cut(s) 2065, 2097, 3865, 3897
BauI CACGAG 4 cut(s) 327, 336, 2742, 3996
BbrPI CACGTG 3 cut(s) 172, 1756, 2852
BbsI GAAGAC 4 cut(s) 1217, 3263, 3378, 3414
Bbv12I GWGCWC 5 cut(s) 328, 400, 1085, 3661, 4494
BceAI ACGGC 1 cut(s) 3086
BcgI CGANNNNNNTGC 2 cut(s) 1802, 1836
BciT130I CCWGG 7 cut(s) 2913, 3118, 3699, 3761, 4059, 4167, 4831
BciVI GTATCC 1 cut(s) 3002
BcnI CCSGG 5 cut(s) 160, 404, 3042, 4654, 4694
BcoDI GTCTC 9 cut(s) 206, 1431, 3089, 3218, 3596, 3697, 4200, 4683, 4739
BfaI CTAG 6 cut(s) 1914, 2237, 4280, 4344, 4628, 4859
BfmI CTRYAG 2 cut(s) 1473, 1746
BfoI RGCGCY 1 cut(s) 255
BfuAI ACCTGC 1 cut(s) 779
BfuI GTATCC 1 cut(s) 3002
BglI GCCNNNNNGGC 2 cut(s) 983, 2058
BlnI CCTAGG 1 cut(s) 4343
BlpI GCTNAGC 2 cut(s) 2169, 2664
BmcAI AGTACT 1 cut(s) 3883
Bme18I GGWCC 6 cut(s) 19, 377, 992, 1716, 2986, 4163
BmeT110I CYCGRG 6 cut(s) 762, 1194, 2699, 2922, 3131, 4317
BmgBI CACGTC 1 cut(s) 1562
BmrI ACTGGG 3 cut(s) 2048, 3370, 4516
BmuI ACTGGG 3 cut(s) 2048, 3370, 4516
BpiI GAAGAC 4 cut(s) 1217, 3263, 3378, 3414
BpmI CTGGAG 9 cut(s) 1020, 1500, 1613, 1827, 2357, 2934, 3591, 3720, 3994
Bpu1102I GCTNAGC 2 cut(s) 2169, 2664
BpuEI CTTGAG 3 cut(s) 337, 599, 4478
BpuMI CCSGG 5 cut(s) 160, 404, 3042, 4654, 4694
BsaAI YACGTR 6 cut(s) 172, 1304, 1756, 2420, 2504, 2852
BsaBI GATNNNNATC 2 cut(s) 1635, 1915
BsaHI GRCGYC 2 cut(s) 2475, 3377
BsaI GGTCTC 5 cut(s) 206, 3218, 3697, 4683, 4739
BsaWI WCCGGW 2 cut(s) 994, 3864
BsaXI ACNNNNNCTCC 6 cut(s) 4088, 4107, 4118, 4137, 4680, 4710
Bse118I RCCGGY 2 cut(s) 994, 4433
Bse3DI GCAATG 4 cut(s) 715, 850, 1269, 2272
Bse8I GATNNNNATC 2 cut(s) 1635, 1915
BseAI TCCGGA 1 cut(s) 3864
BseBI CCWGG 7 cut(s) 2913, 3118, 3699, 3761, 4059, 4167, 4831
BseJI GATNNNNATC 2 cut(s) 1635, 1915
BseMI GCAATG 4 cut(s) 715, 850, 1269, 2272
BseMII CTCAG 7 cut(s) 217, 687, 973, 2045, 2183, 2818, 3644
BseRI GAGGAG 2 cut(s) 1632, 4086
BseSI GKGCMC 3 cut(s) 1085, 3661, 4269
BseYI CCCAGC 1 cut(s) 2141
Bsh1236I CGCG 3 cut(s) 46, 1374, 1376
BshFI GGCC 9 cut(s) 90, 1740, 1773, 2303, 2790, 3073, 3927, 4267, 4828
BshTI ACCGGT 1 cut(s) 994
BsiHKAI GWGCWC 5 cut(s) 328, 400, 1085, 3661, 4494
BsiHKCI CYCGRG 6 cut(s) 762, 1194, 2699, 2922, 3131, 4317
BsiSI CCGG 9 cut(s) 160, 246, 404, 995, 3042, 3865, 4434, 4653, 4693
BslFI GGGAC 8 cut(s) 5, 141, 585, 1005, 3023, 3558, 3661, 4517
BsmAI GTCTC 9 cut(s) 206, 1431, 3089, 3218, 3596, 3697, 4200, 4683, 4739
BsmBI CGTCTC 1 cut(s) 4200
BsmFI GGGAC 8 cut(s) 5, 141, 585, 1005, 3023, 3558, 3661, 4517
BsmI GAATGC 1 cut(s) 2257
BsnI GGCC 9 cut(s) 90, 1740, 1773, 2303, 2790, 3073, 3927, 4267, 4828
Bso31I GGTCTC 5 cut(s) 206, 3218, 3697, 4683, 4739
BsoBI CYCGRG 6 cut(s) 762, 1194, 2699, 2922, 3131, 4317
Bsp120I GGGCCC 1 cut(s) 4265
Bsp1286I GDGCHC 7 cut(s) 328, 400, 870, 1085, 3661, 4269, 4494
Bsp13I TCCGGA 1 cut(s) 3864
Bsp1407I TGTACA 1 cut(s) 1360
Bsp1720I GCTNAGC 2 cut(s) 2169, 2664
Bsp19I CCATGG 4 cut(s) 91, 628, 1768, 4369
BspANI GGCC 9 cut(s) 90, 1740, 1773, 2303, 2790, 3073, 3927, 4267, 4828
BspCNI CTCAG 7 cut(s) 216, 688, 972, 2044, 2182, 2817, 3645
BspEI TCCGGA 1 cut(s) 3864
BspFNI CGCG 3 cut(s) 46, 1374, 1376
BspHI TCATGA 1 cut(s) 2098
BspMAI CTGCAG 1 cut(s) 1477
BspMI ACCTGC 1 cut(s) 779
BspQI GCTCTTC 2 cut(s) 1548, 4482
BspTNI GGTCTC 5 cut(s) 206, 3218, 3697, 4683, 4739
BsrBI CCGCTC 1 cut(s) 335
BsrDI GCAATG 4 cut(s) 715, 850, 1269, 2272
BsrFI RCCGGY 2 cut(s) 994, 4433
BsrGI TGTACA 1 cut(s) 1360
BssAI RCCGGY 2 cut(s) 994, 4433
BssNI GRCGYC 2 cut(s) 2475, 3377
BssSI CACGAG 4 cut(s) 327, 336, 2742, 3996
BssT1I CCWWGG 6 cut(s) 91, 628, 1768, 2825, 4343, 4369
Bst2BI CACGAG 4 cut(s) 327, 336, 2742, 3996
Bst2UI CCWGG 7 cut(s) 2913, 3118, 3699, 3761, 4059, 4167, 4831
Bst6I CTCTTC 4 cut(s) 429, 1548, 2292, 4482
BstACI GRCGYC 2 cut(s) 2475, 3377
BstAPI GCANNNNNTGC 4 cut(s) 485, 1268, 1472, 2402
BstAUI TGTACA 1 cut(s) 1360
BstBAI YACGTR 6 cut(s) 172, 1304, 1756, 2420, 2504, 2852
BstDSI CCRYGG 6 cut(s) 91, 628, 1097, 1768, 3305, 4369
BstFNI CGCG 3 cut(s) 46, 1374, 1376
BstH2I RGCGCY 1 cut(s) 255
BstHHI GCGC 6 cut(s) 254, 1376, 1519, 2161, 2312, 3747
BstMAI GTCTC 9 cut(s) 206, 1431, 3089, 3218, 3596, 3697, 4200, 4683, 4739
BstNI CCWGG 7 cut(s) 2913, 3118, 3699, 3761, 4059, 4167, 4831
BstNSI RCATGY 3 cut(s) 897, 2798, 4769
BstSFI CTRYAG 2 cut(s) 1473, 1746
BstSLI GKGCMC 3 cut(s) 1085, 3661, 4269
BstUI CGCG 3 cut(s) 46, 1374, 1376
BstV2I GAAGAC 4 cut(s) 1217, 3263, 3378, 3414
BstXI CCANNNNNNTGG 3 cut(s) 987, 2532, 3117
BsuI GTATCC 1 cut(s) 3002
BsuRI GGCC 9 cut(s) 90, 1740, 1773, 2303, 2790, 3073, 3927, 4267, 4828
BtgI CCRYGG 6 cut(s) 91, 628, 1097, 1768, 3305, 4369
BtrI CACGTC 1 cut(s) 1562
BtsI GCAGTG 5 cut(s) 972, 1573, 1830, 2061, 4338
BveI ACCTGC 1 cut(s) 779
CaiI CAGNNNCTG 1 cut(s) 3004
CciI TCATGA 1 cut(s) 2098
CfoI GCGC 6 cut(s) 254, 1376, 1519, 2161, 2312, 3747
Cfr10I RCCGGY 2 cut(s) 994, 4433
CseI GACGC 1 cut(s) 3366
Csp6I GTAC 9 cut(s) 621, 1065, 1154, 1361, 1765, 2501, 2865, 3068, 3882
CspAI ACCGGT 1 cut(s) 994
CspCI CAANNNNNGTGG 2 cut(s) 1806, 1841
CviQI GTAC 9 cut(s) 621, 1065, 1154, 1361, 1765, 2501, 2865, 3068, 3882
DraI TTTAAA 1 cut(s) 1234
DraIII CACNNNGTG 3 cut(s) 517, 1098, 1679
EaeI YGGCCR 4 cut(s) 88, 1771, 2301, 3071
Eam1104I CTCTTC 4 cut(s) 429, 1548, 2292, 4482
EarI CTCTTC 4 cut(s) 429, 1548, 2292, 4482
Ecl136II GAGCTC 3 cut(s) 326, 398, 4492
Eco130I CCWWGG 6 cut(s) 91, 628, 1768, 2825, 4343, 4369
Eco24I GRGCYC 5 cut(s) 328, 400, 870, 4269, 4494
Eco31I GGTCTC 5 cut(s) 206, 3218, 3697, 4683, 4739
Eco32I GATATC 2 cut(s) 1618, 2538
Eco47I GGWCC 6 cut(s) 19, 377, 992, 1716, 2986, 4163
Eco53kI GAGCTC 3 cut(s) 326, 398, 4492
Eco57I CTGAAG 4 cut(s) 1211, 1296, 2994, 3306
Eco72I CACGTG 3 cut(s) 172, 1756, 2852
Eco88I CYCGRG 6 cut(s) 762, 1194, 2699, 2922, 3131, 4317
EcoICRI GAGCTC 3 cut(s) 326, 398, 4492
EcoO109I RGGNCCY 2 cut(s) 19, 4265
EcoRI GAATTC 2 cut(s) 1188, 2966
EcoRII CCWGG 7 cut(s) 2911, 3116, 3697, 3759, 4057, 4165, 4829
EcoRV GATATC 2 cut(s) 1618, 2538
EcoT14I CCWWGG 6 cut(s) 91, 628, 1768, 2825, 4343, 4369
EcoT22I ATGCAT 1 cut(s) 1051
EcoT38I GRGCYC 5 cut(s) 328, 400, 870, 4269, 4494
ErhI CCWWGG 6 cut(s) 91, 628, 1768, 2825, 4343, 4369
Esp3I CGTCTC 1 cut(s) 4200
FalI AAGNNNNNCTT 6 cut(s) 96, 128, 705, 737, 4027, 4059
FaqI GGGAC 8 cut(s) 5, 141, 585, 1005, 3023, 3558, 3661, 4517
FauI CCCGC 2 cut(s) 1719, 4255
FauNDI CATATG 3 cut(s) 1959, 2292, 3558
FblI GTMKAC 1 cut(s) 3673
FriOI GRGCYC 5 cut(s) 328, 400, 870, 4269, 4494
FspBI CTAG 6 cut(s) 1914, 2237, 4280, 4344, 4628, 4859
FspI TGCGCA 1 cut(s) 2160
GlaI GCGC 6 cut(s) 253, 1375, 1518, 2160, 2311, 3746
GsaI CCCAGC 1 cut(s) 2145
GsuI CTGGAG 9 cut(s) 1020, 1500, 1613, 1827, 2357, 2934, 3591, 3720, 3994
HaeII RGCGCY 1 cut(s) 255
HaeIII GGCC 9 cut(s) 90, 1740, 1773, 2303, 2790, 3073, 3927, 4267, 4828
HapII CCGG 9 cut(s) 160, 246, 404, 995, 3042, 3865, 4434, 4653, 4693
HgaI GACGC 1 cut(s) 3366
HhaI GCGC 6 cut(s) 254, 1376, 1519, 2161, 2312, 3747
Hin1I GRCGYC 2 cut(s) 2475, 3377
Hin6I GCGC 6 cut(s) 252, 1374, 1517, 2159, 2310, 3745
HinP1I GCGC 6 cut(s) 252, 1374, 1517, 2159, 2310, 3745
HindIII AAGCTT 1 cut(s) 4295
HpaII CCGG 9 cut(s) 160, 246, 404, 995, 3042, 3865, 4434, 4653, 4693
Hpy99I CGWCG 2 cut(s) 296, 1243
Hsp92I GRCGYC 2 cut(s) 2475, 3377
HspAI GCGC 6 cut(s) 252, 1374, 1517, 2159, 2310, 3745
Kpn2I TCCGGA 1 cut(s) 3864
KroI GCCGGC 1 cut(s) 4433
KroNI GCCGGC 1 cut(s) 4435
LguI GCTCTTC 2 cut(s) 1548, 4482
LmnI GCTCC 8 cut(s) 323, 395, 730, 877, 1808, 2915, 3157, 4096
MaeI CTAG 6 cut(s) 1914, 2237, 4280, 4344, 4628, 4859
MbiI CCGCTC 1 cut(s) 335
MhlI GDGCHC 7 cut(s) 328, 400, 870, 1085, 3661, 4269, 4494
MlsI TGGCCA 2 cut(s) 1773, 2303
MluNI TGGCCA 2 cut(s) 1773, 2303
MlyI GAGTC 6 cut(s) 21, 2238, 2348, 2796, 3598, 3643
MmeI TCCRAC 8 cut(s) 162, 373, 2177, 3220, 4093, 4130, 4141, 4285
Mox20I TGGCCA 2 cut(s) 1773, 2303
Mph1103I ATGCAT 1 cut(s) 1051
MroI TCCGGA 1 cut(s) 3864
MroNI GCCGGC 1 cut(s) 4433
MroXI GAANNNNTTC 4 cut(s) 1125, 1224, 2520, 4225
MscI TGGCCA 2 cut(s) 1773, 2303
MseI TTAA 9 cut(s) 363, 1233, 1257, 1695, 2040, 2438, 2564, 3098, 4698
MslI CAYNNNNRTG 8 cut(s) 601, 1962, 3234, 4023, 4368, 4374, 4577, 4726
Msp20I TGGCCA 2 cut(s) 1773, 2303
MspA1I CMGCKG 3 cut(s) 1478, 1664, 2141
MspI CCGG 9 cut(s) 160, 246, 404, 995, 3042, 3865, 4434, 4653, 4693
MssI GTTTAAAC 1 cut(s) 1234
Mva1269I GAATGC 1 cut(s) 2257
MvaI CCWGG 7 cut(s) 2913, 3118, 3699, 3761, 4059, 4167, 4831
MvnI CGCG 3 cut(s) 46, 1374, 1376
NaeI GCCGGC 1 cut(s) 4435
NciI CCSGG 5 cut(s) 160, 404, 3042, 4654, 4694
NcoI CCATGG 4 cut(s) 91, 628, 1768, 4369
NdeI CATATG 3 cut(s) 1959, 2292, 3558
NgoMIV GCCGGC 1 cut(s) 4433
NmuCI GTSAC 5 cut(s) 931, 2504, 3356, 3508, 3664
NsbI TGCGCA 1 cut(s) 2160
NsiI ATGCAT 1 cut(s) 1051
NspI RCATGY 3 cut(s) 897, 2798, 4769
OliI CACNNNNGTG 2 cut(s) 4577, 4726
PaeR7I CTCGAG 3 cut(s) 762, 2699, 2922
PagI TCATGA 1 cut(s) 2098
PaqCI CACCTGC 1 cut(s) 779
PciI ACATGT 1 cut(s) 4765
PciSI GCTCTTC 2 cut(s) 1548, 4482
PcsI WCGNNNNNNNCGW 1 cut(s) 334
PctI GAATGC 1 cut(s) 2257
PdiI GCCGGC 1 cut(s) 4435
PdmI GAANNNNTTC 4 cut(s) 1125, 1224, 2520, 4225
PfeI GAWTC 9 cut(s) 736, 920, 1040, 2773, 3001, 3553, 3633, 3907, 3992
PflFI GACNNNGTC 1 cut(s) 3362
PflMI CCANNNNNTGG 3 cut(s) 1098, 2831, 3587
PfoI TCCNGGA 1 cut(s) 2911
PinAI ACCGGT 1 cut(s) 994
PleI GAGTC 6 cut(s) 20, 2237, 2347, 2796, 3598, 3643
PmaCI CACGTG 3 cut(s) 172, 1756, 2852
PmeI GTTTAAAC 1 cut(s) 1234
PmlI CACGTG 3 cut(s) 172, 1756, 2852
PpsI GAGTC 6 cut(s) 20, 2237, 2347, 2796, 3598, 3643
Ppu21I YACGTR 6 cut(s) 172, 1304, 1756, 2420, 2504, 2852
PpuMI RGGWCCY 1 cut(s) 19
PscI ACATGT 1 cut(s) 4765
PshBI ATTAAT 2 cut(s) 363, 2040
Psp124BI GAGCTC 3 cut(s) 328, 400, 4494
Psp5II RGGWCCY 1 cut(s) 19
Psp6I CCWGG 7 cut(s) 2911, 3116, 3697, 3759, 4057, 4165, 4829
PspCI CACGTG 3 cut(s) 172, 1756, 2852
PspFI CCCAGC 1 cut(s) 2141
PspGI CCWGG 7 cut(s) 2911, 3116, 3697, 3759, 4057, 4165, 4829
PspOMI GGGCCC 1 cut(s) 4265
PspPPI RGGWCCY 1 cut(s) 19
PspXI VCTCGAGB 1 cut(s) 762
PstI CTGCAG 1 cut(s) 1477
PstNI CAGNNNCTG 1 cut(s) 3004
PsyI GACNNNGTC 1 cut(s) 3362
PvuII CAGCTG 3 cut(s) 1478, 1664, 2141
RsaI GTAC 9 cut(s) 622, 1066, 1155, 1362, 1766, 2502, 2866, 3069, 3883
RsaNI GTAC 9 cut(s) 621, 1065, 1154, 1361, 1765, 2501, 2865, 3068, 3882
RseI CAYNNNNRTG 8 cut(s) 601, 1962, 3234, 4023, 4368, 4374, 4577, 4726
SacI GAGCTC 3 cut(s) 328, 400, 4494
SapI GCTCTTC 2 cut(s) 1548, 4482
SaqAI TTAA 9 cut(s) 363, 1233, 1257, 1695, 2040, 2438, 2564, 3098, 4698
ScaI AGTACT 1 cut(s) 3883
SchI GAGTC 6 cut(s) 21, 2238, 2348, 2796, 3598, 3643
SduI GDGCHC 7 cut(s) 328, 400, 870, 1085, 3661, 4269, 4494
SfcI CTRYAG 2 cut(s) 1473, 1746
Sfr274I CTCGAG 3 cut(s) 762, 2699, 2922
SinI GGWCC 6 cut(s) 19, 377, 992, 1716, 2986, 4163
SlaI CTCGAG 3 cut(s) 762, 2699, 2922
SmiMI CAYNNNNRTG 8 cut(s) 601, 1962, 3234, 4023, 4368, 4374, 4577, 4726
SmlI CTYRAG 6 cut(s) 352, 578, 762, 2699, 2922, 4493
SmoI CTYRAG 6 cut(s) 352, 578, 762, 2699, 2922, 4493
SspMI CTAG 6 cut(s) 1914, 2237, 4280, 4344, 4628, 4859
SstI GAGCTC 3 cut(s) 328, 400, 4494
StyI CCWWGG 6 cut(s) 91, 628, 1768, 2825, 4343, 4369
TatI WGTACW 3 cut(s) 620, 1360, 3881
TauI GCSGC 6 cut(s) 90, 242, 252, 335, 843, 4828
TfiI GAWTC 9 cut(s) 736, 920, 1040, 2773, 3001, 3553, 3633, 3907, 3992
Tru1I TTAA 9 cut(s) 363, 1233, 1257, 1695, 2040, 2438, 2564, 3098, 4698
Tru9I TTAA 9 cut(s) 363, 1233, 1257, 1695, 2040, 2438, 2564, 3098, 4698
TseFI GTSAC 5 cut(s) 931, 2504, 3356, 3508, 3664
Tsp45I GTSAC 5 cut(s) 931, 2504, 3356, 3508, 3664
TspGWI ACGGA 9 cut(s) 22, 290, 557, 959, 1086, 2978, 2984, 4219, 4432
Tth111I GACNNNGTC 1 cut(s) 3362
Van91I CCANNNNNTGG 3 cut(s) 1098, 2831, 3587
VneI GTGCAC 2 cut(s) 1081, 3657
VpaK11BI GGWCC 6 cut(s) 19, 377, 992, 1716, 2986, 4163
VspI ATTAAT 2 cut(s) 363, 2040
XapI RAATTY 6 cut(s) 1122, 1188, 2025, 2618, 2966, 4805
XbaI TCTAGA 3 cut(s) 1913, 2236, 4858
XceI RCATGY 3 cut(s) 897, 2798, 4769
XcmI CCANNNNNNNNNTGG 8 cut(s) 826, 1894, 2832, 3236, 3584, 3588, 3780, 3969
XhoI CTCGAG 3 cut(s) 762, 2699, 2922
XmaJI CCTAGG 1 cut(s) 4343
XmiI GTMKAC 1 cut(s) 3673
XmnI GAANNNNTTC 4 cut(s) 1125, 1224, 2520, 4225
XspI CTAG 6 cut(s) 1914, 2237, 4280, 4344, 4628, 4859
ZraI GACGTC 1 cut(s) 2476
ZrmI AGTACT 1 cut(s) 3883
Zsp2I ATGCAT 1 cut(s) 1051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.