FvH4_7g03470

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010072
Physical Location & Seq
Reverse (-)
3816565 .. 3829745
13181 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g03470.t4

Sequence Viewer

Length: 4899 bp
ATGAAAGTGCCGTGCTGCTCGGTCTGCCAGACGCGTTACGATGAGGAAGAGAGAGTGCCGCTGCTGCTGCAGTGCGGCCACGGCTTCTGCAAAGACTGCTTGTCAAAAATGTTCTCGGCGTGTCCTGATACCACGCTCGTCTGCCCTAGGTGCCGCCACGTCTCCGTCGTCGGCAACTCAGTTCAGGCGCTCCGCAAGAACTTTGCCGTGCTCGCGCTGATTCACTCCAGCACCTCCAACGGCGTCGTCTCGTCCTCCGCCGCCGCCGCCAATTTTGATTGCGATTATACGGACGACGAGGACGGCGATGAGGATGAGGACGACGACAATGACGAGGCCTCGCGCCGCCGATGCAGCCGCGGATCCCACGCGTCAAGCTCCGGCGGCTGCGGCCCCGTGATTGAGGTGGCGGTGCACCCGGAGTTGAGGTTTTTGAAGAGGACAGGGGAGGGGAGGCAGGCTGGGGTTGAAATGTGGACGGCGGTTATTGGAGGGAGTGGGGGGCGCTGCCGGCACCGTGTTGCCGTGAAGAAGGTGGCGGTAGTGGCGGAGGAGACTAGTATGGAGTGGGTGATGGGGCAACTAGAGAACTTGCGGAGGGCATCCATGTGGTGCAGGAATGTGTGTACATTTCATGGGGCTACGAAGAGTGAAGGCACTTTGTGCCTTGTTATGGACAAATGTTATGGCTCTGTTCAATCCGAGATGGACCGCAATGAGGGCAGACTCACTTTAGAGCAAATCTTAAGATATGGTGCTGACATTGCACGAGGAGTAGCTGAACTCCATGCTGCAGGTGTTGTTTGTATGAATTTAAAACCATCCAATTTGCTTTTGGATGCCAATGGTCATGCTGTGGTTTCTGATTATGGAGTTGCTGCAATTCTTAAAAAACCTTCCTGCCGGAAAACTCGATCAGAAATTGACACCTCAAGGGTCCATTCGTGTATGGAGTGTACAATGCTGAGTCCTCACTATGCAGCTCCAGAGGCATGGGAGCCGGTGAAGAAGTCACTGAATCCATTCTGGGATGAACCTATTGGTATCTCTGCAGAGTCAGATGCCTGGAGTTTTGGTTGTACATTGGTAGAAATGTGCACTGGTTCCATCCCATGGGCTGGTTTGAGCACAGAGGAAATTTACAAGGCGGTGGTCAAGGCTCGAAAACTGCCTCCACAATATGCAAGTGTAGTAGGTGTTGGAATACCTAGGGAATTATGGAAAATGATTGGTGAGTGCCTGCAGTACAAGGCATCAAAAAGACCATCCTTCAATTTAATGCTAGCAACATTCCTCCGCCATTTGCAGGAGATACCACGGAGCCCTCCTGCTAGTCCTGATAATGAAGTATCTAAAAGCTTGGGATCCAATGTGAAGCAACAATCGCCTCTATCCTATTCAAGAGTATTTCAGGGTGATCCCGCCCTTCTGCATAGACTTGTGTCCGAAGGGGATGTAAATGGTGTTAGAGATCTGCTTGGAAAAGCTGCAGTGGGAAGTGACAACAGTGTAATATCGTCTCTATTAGAAGCTCAAAATGCTGATGGCCAAACTGCTCTTCATTTGGCCTGCAGACGTGGTAGTGCAGAACTTGTTGATGCTATTTTGGAGTATAGAGAGGCAAATGTAGATGTGTTGGACAAAGATGGGGATCCTCCCCTTGTTTTTGCTCTAGTAGCTGGATCCCCAGAGTGTGTTCATGTTCTCATTAAAAGAGGTGCTAATGTGAGATCTCGGTTGCGGGAGGGCTTTGGTCCCTCTGTTGCTCATGTTTGTGCTTATCATGGCCAGCCTGATTGTATGCGTGAGTTACTAATGGCTGGAGCTGATCCTAATGCGGTGGATGAGGAAGGGGAATCTGTACTCCATAGAGCTATTACCAAGAAGTATACAGATTGTGCACTTGTGGTTTTAGAAAATGGGGGCTGTAGGTCAATGACTGTTCTAAATTCGGAAAAAATGACGCCACTGCACTTGTGTGTGCAAACATGGAATGTTGCTGTTGTTAGAAGATGGGTGGAAGTAGCAACACCAGAGGAGATTGCCGATGCAATTGATATACCAAGTCCAGTTGGCACTGCGCTGTGTATGGCCGCTGCTCTAAAGAAAGATCATGAAATTGAAGGAAGAGAATTGGTGCGTATTTTGCTTGCTTCTCGAGCCGATCCAACTGCTCAAGATGCCCAGAATGGACGAACAGCTTTGCATACAGCTTCAATGGCGAATGATGTTGAGTTGGTGAAGATTATACTTGATGCGGGAGTTGATGTGAACATCCGAAATGCACAAAATACAATTCCACTTCATGTAGCACTGGCCAGAGGGGCGAAGTCATGTGTTGGGCTGCTTCTATCTGCTGGAGCAAATTATAATTTGCAGGATGATGAAGGTGACAATGCTTTCCACATAGCAGCAGATGCAGCAAAAATGATACGTGAAAATCTTGAATGGCTTATTGTCATGCTTAGAAATCCTGATGCTTCTGTTGAAGCCAGAAATCACAGTGGCAAGACATTGCGTGACTTTCTGGAAGCCCTTCCTCGGGAATGGGTATCTGAAGACCTGATGGAGGCACTTGTTAATAGGGGAATTTATCTGTCTCCTACAATATTTGAAGTTGGGGATTGGATCAAATTTAAAAGAAGTATCACAAATCCAGCATATGGATGGCAAGGTGCTAAGCATAGAAGTGTTGGCTTCGTGCAAAGTGTCCCAGATAAGGACAATTTGATAGTATCGTTTTGTTCTGGAGAGGCTCATGAGGCTCGTGTCTTAGCAAATGAAGTTATAAAAGTTATTCCGTTGGACAGGGGACAGCATGTCCAGCTTAAGCCCGATGTTAAAGAGCCAAGGTTTGGGTGGCGTGGGCAATCACGTGATAGCATAGGTACTGTATTATGCGTTGATGATGATGGTATCCTACGTGTTGGGTTTCCTGGAGCCTCCAGAGGATGGAAAGCTGATCCTGCTGAAATGGAGAGAGTTGAAGAATTCAAGGTTGGCGACTGGGTTCGTATACGCCCGACTCTTACAACAGCAAAGCATGGATTAGGATCTGTTACACCTGGGAGCATTGGTATTGTGTATTGTATAAGACCAGATAGTAGTCTGTTGTTAGAGTTGAGTTATCTTCCAACCCCTTGGCATTGTGAACCAGAGGAGGTTGAGCCTGTAATTCCCTTCCGGATTGGTGACAGGGTATGTGTGAAGCGATCGGTTGCAGAACCTAGGTACGCATGGGGTGGTGAAACCCACCATAGTGTTGGAAGAATAAGTGAGATAGAGAATGATGGCCTCCTGGTTATTGAAATTCCAAACCGACCAATATCATGGCAGGCTGATCCTTCCGACATGGAGAAATTGGAAGATTTCAAGGTAGGAGACTGGGTTAGAGTCAAAGCTTCCGTACCCTCCCCCAAATACGGATGGGAGGATATTACAAGGAATAGTATTGGGATAATCCATAGCTTGGAGGAAGATGGTGATATGGGTGTTGCTTTTTGCTTCAGGAGCAAGCCTTTTTCTTGTTCTGTCACAGATGTGGAGAAGCTACCGCCATTTGAGCTGGGACAAGAAATTCATATTCTGTCATCTGTCACTCAGCCACGACTTGGATGGTCAAATGAGAGTCCGGCAACTGTTGGGAAAATAAATAGAATTGACATGGATGGAGCTTTAAATGTAAGGGTGCCTGGCAGACAGAGCTTGTGGAAAGTTTCTCCAGGAGATGCTGAACGACTCTCTGGATTTGAAGTTGGTGACTGGGTACGATCGAAACCAAGCCTGGGAACACGACCAAGTTATGACTGGAACAGTATTGGAAAAGAAAGTTTAGCAGTTGTGCATAGTGTACAAGACACAGGTTATCTTGAGTTGGCTTGCTGTTTCCGTAAAGGCAGGTGGATAACTCATTACACAGATGTTGAAAAGGTTCCATCCTTCAAAGTTGGCCAATATGTTCGGTTTCGTATTGGTTTAGTTGAACCAAGATGGGGATGGAGAGGGGCTCAACCTGATTCTAGAGGCATTATAACTAGTATTCATGCTGATGGGGAAGTGAGAGTGGCTTTCTCTGGTTTGCCAGGGTTGTGGAGAGGAGATCCTGCTGATTTTGAGATTGAGCAGATTTTTGAAGTTGGAGAGTGGGTGAAATTAGAAGATCATGCAAATATGTGGAAATCCGTTGGGCCAGGGAGTGTTGGTGTGGTGCAAGGATTAGGATATGAGGAAGATAAATGGGATGGAACCACTTTTGTTGGATTCTGCGGGGAGCAAGAGAGGTGGATTGGGCCTACTTCTGATCTTGCAAGGGCAAACAAGCTCATGGTTGGCCAGAAGGTTAGGGTTAAATTATCTGTCAAGCAGCCGCGATTTGGATGGTCAGGCCACAGCCACGCAAGTCTTGGAACTATAGCCGGGATTGATGCCGATGGAAAGCTGAGAATATACACTCCAAGTGGTTCAAAAGCTTGGATGTTGGATCCAACAGAAGTGCAACTTGTGGAGGAAGAGGAGCTTCATATTGGAGACTGGGTTAGGGTTAAACCATCAGTATCAACCCCAACCCACCAATGGGGAGAGGTGAATCGTTCGAGTGTTGGGGTGGTGCATCGTATTGAAAATGAGGAGCTTTGGGTAGCTTTTTGTTTCACAGAAAGGTTATGGCTTTGCAAGGCATTGGAAATGGAACGGGTTAGGCCCTTCAGAGTGGGGGACAAAGTGAGGATTAGAGAAGGGTTGGTGTCTCCACGGTGGGGTTGGGGAATGGAGACACATGCCAGCAAAGGAGAGGTAGTTGGGGTGGATGCAAATGGAAAGTTAAGAATTAAGTTCCGGTGGAGAGAAGGGAGACCATGGATTGGAGATCCTGCAGATGTCGCTATTGATGAGAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Pkinase zf-C3HC4 zf-RING_2 zf-RING_5

Protein Analysis

1633

Amino Acids

179.92

Weight (kDa)

5.82

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 5 - 52 9.1e-06 Ring finger domain
zf-RING_UBOX PF13445 6 - 49 3e-06 RING-type zinc-finger
PK_Tyr_Ser-Thr PF07714 144 - 428 5.4e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 172 - 428 9.1e-29 Protein kinase domain
Ank_2 PF12796 477 - 578 6.3e-13 Ankyrin repeats (3 copies)
Ank PF00023 515 - 548 6.5e-06 Ankyrin repeat
Ank_4 PF13637 590 - 634 5.5e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 590 - 670 9.3e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 709 - 794 3.3e-14 Ankyrin repeats (3 copies)
Ank PF00023 730 - 761 1.3e-06 Ankyrin repeat
Ank_4 PF13637 735 - 784 5.2e-07 Ankyrin repeats (many copies)
SH3_15 PF18346 927 - 992 9.3e-14 Mind bomb SH3 repeat domain
SH3_15 PF18346 1061 - 1118 3.3e-08 Mind bomb SH3 repeat domain
SH3_15 PF18346 1124 - 1168 5.9e-06 Mind bomb SH3 repeat domain
SH3_15 PF18346 1180 - 1242 4.1e-07 Mind bomb SH3 repeat domain
SH3_15 PF18346 1250 - 1370 3.3e-16 Mind bomb SH3 repeat domain
SH3_15 PF18346 1377 - 1498 1.2e-16 Mind bomb SH3 repeat domain
SH3_15 PF18346 1507 - 1626 1.8e-14 Mind bomb SH3 repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2370, 2789, 4031
AarI CACCTGC 2 cut(s) 785, 3888
Acc36I ACCTGC 2 cut(s) 785, 3888
AccB1I GGYRCC 3 cut(s) 150, 513, 3688
AccB7I CCANNNNNTGG 8 cut(s) 1113, 1118, 2663, 2855, 2952, 3469, 3611, 4859
AccI GTMKAC 2 cut(s) 1889, 3016
AccII CGCG 6 cut(s) 34, 215, 343, 360, 371, 4369
AccIII TCCGGA 1 cut(s) 3183
AcoI YGGCCR 7 cut(s) 76, 1546, 1786, 2091, 2316, 3949, 4330
AcsI RAATTY 8 cut(s) 811, 1137, 1948, 2589, 2633, 2990, 3309, 3576
AcuI CTGAAG 3 cut(s) 2577, 3490, 4687
AcvI CACGTG 1 cut(s) 2876
AcyI GRCGYC 2 cut(s) 243, 1964
AdeI CACNNNGTG 1 cut(s) 663
AflII CTTAAG 2 cut(s) 745, 2828
AflIII ACRYGT 3 cut(s) 32, 369, 2923
AhdI GACNNNNNGTC 2 cut(s) 100, 2819
AhlI ACTAGT 2 cut(s) 557, 4034
AjiI CACGTC 2 cut(s) 160, 1577
AjnI CCWGG 9 cut(s) 1064, 2935, 3064, 3297, 3691, 3721, 3783, 4081, 4189
AjuI GAANNNNNNNTTGG 4 cut(s) 1542, 1574, 2982, 3014
AleI CACNNNNGTG 3 cut(s) 1977, 3258, 3539
Alw21I GWGCWC 5 cut(s) 213, 417, 1100, 1130, 1903
Alw44I GTGCAC 3 cut(s) 413, 1096, 1899
Ama87I CYCGRG 2 cut(s) 2157, 2541
Aor13HI TCCGGA 1 cut(s) 3183
ApaLI GTGCAC 3 cut(s) 413, 1096, 1899
ApoI RAATTY 8 cut(s) 811, 1137, 1948, 2589, 2633, 2990, 3309, 3576
Asp700I GAANNNNTTC 3 cut(s) 1022, 2535, 3930
AspA2I CCTAGG 3 cut(s) 146, 1208, 3227
AspLEI GCGC 5 cut(s) 190, 217, 345, 507, 2083
AspS9I GGNCC 7 cut(s) 392, 709, 937, 1754, 4187, 4289, 4698
AsuC2I CCSGG 2 cut(s) 419, 4417
AsuNHI GCTAGC 1 cut(s) 1282
AvaI CYCGRG 2 cut(s) 2157, 2541
AvaII GGWCC 3 cut(s) 709, 937, 1754
AvrII CCTAGG 3 cut(s) 146, 1208, 3227
BaeGI GKGCMC 3 cut(s) 417, 1100, 1903
BalI TGGCCA 5 cut(s) 1548, 1788, 2318, 3951, 4332
BamHI GGATCC 5 cut(s) 362, 1364, 1651, 1682, 4480
BanI GGYRCC 3 cut(s) 150, 513, 3688
BanII GRGCYC 2 cut(s) 1325, 4009
BauI CACGAG 2 cut(s) 768, 2766
BbrPI CACGTG 1 cut(s) 2876
BbsI GAAGAC 1 cut(s) 2565
Bbv12I GWGCWC 5 cut(s) 213, 417, 1100, 1130, 1903
BceAI ACGGC 6 cut(s) 97, 191, 256, 319, 495, 509
BcgI CGANNNNNNTGC 2 cut(s) 2153, 2187
BciT130I CCWGG 9 cut(s) 1066, 2937, 3066, 3299, 3693, 3723, 3785, 4083, 4191
BciVI GTATCC 1 cut(s) 2927
BcnI CCSGG 2 cut(s) 419, 4417
BcuI ACTAGT 2 cut(s) 557, 4034
BfmI CTRYAG 9 cut(s) 68, 792, 1050, 1241, 1488, 1570, 1927, 4410, 4869
BfoI RGCGCY 2 cut(s) 191, 508
BfrI CTTAAG 2 cut(s) 745, 2828
BfuAI ACCTGC 2 cut(s) 785, 3888
BfuI GTATCC 1 cut(s) 2927
BglI GCCNNNNNGGC 1 cut(s) 2324
BglII AGATCT 2 cut(s) 1471, 1730
BlnI CCTAGG 3 cut(s) 146, 1208, 3227
BlpI GCTNAGC 1 cut(s) 2679
Bme18I GGWCC 3 cut(s) 709, 937, 1754
BmeRI GACNNNNNGTC 2 cut(s) 100, 2819
BmeT110I CYCGRG 2 cut(s) 2157, 2541
BmgBI CACGTC 2 cut(s) 160, 1577
BmgT120I GGNCC 7 cut(s) 392, 709, 937, 1754, 4187, 4289, 4698
BmrI ACTGGG 4 cut(s) 3016, 3394, 3772, 4540
BmtI GCTAGC 1 cut(s) 1286
BmuI ACTGGG 4 cut(s) 3016, 3394, 3772, 4540
BoxI GACNNNNGTC 1 cut(s) 1441
BpiI GAAGAC 1 cut(s) 2565
BplI GAGNNNNNCTC 2 cut(s) 3991, 4023
BpmI CTGGAG 9 cut(s) 211, 969, 1087, 1842, 2379, 2769, 2929, 2958, 3705
Bpu1102I GCTNAGC 1 cut(s) 2679
BpuEI CTTGAG 3 cut(s) 916, 2160, 3890
BpuMI CCSGG 2 cut(s) 419, 4417
BsaAI YACGTR 3 cut(s) 2435, 2876, 2924
BsaBI GATNNNNATC 2 cut(s) 1650, 3052
BsaHI GRCGYC 2 cut(s) 243, 1964
BsaI GGTCTC 1 cut(s) 4843
BsaWI WCCGGW 2 cut(s) 3183, 4833
Bse118I RCCGGY 2 cut(s) 510, 1000
Bse1I ACTGG 8 cut(s) 1105, 2069, 2319, 3011, 3389, 3767, 3812, 4535
Bse3DI GCAATG 3 cut(s) 721, 762, 2513
Bse8I GATNNNNATC 2 cut(s) 1650, 3052
BseAI TCCGGA 1 cut(s) 3183
BseBI CCWGG 9 cut(s) 1066, 2937, 3066, 3299, 3693, 3723, 3785, 4083, 4191
BseJI GATNNNNATC 2 cut(s) 1650, 3052
BseMI GCAATG 3 cut(s) 721, 762, 2513
BseMII CTCAG 4 cut(s) 192, 956, 3614, 4430
BseNI ACTGG 8 cut(s) 1105, 2069, 2319, 3011, 3389, 3767, 3812, 4535
BseRI GAGGAG 7 cut(s) 566, 786, 2051, 3173, 4110, 4526, 4640
BseSI GKGCMC 3 cut(s) 417, 1100, 1903
BseYI CCCAGC 2 cut(s) 461, 3565
BsgI GTGCAG 3 cut(s) 634, 1605, 1955
Bsh1236I CGCG 6 cut(s) 34, 215, 343, 360, 371, 4369
Bsh1285I CGRYCG 2 cut(s) 3215, 3773
BshNI GGYRCC 3 cut(s) 150, 513, 3688
BsiEI CGRYCG 2 cut(s) 3215, 3773
BsiHKAI GWGCWC 5 cut(s) 213, 417, 1100, 1130, 1903
BsiHKCI CYCGRG 2 cut(s) 2157, 2541
BsiSI CCGG 9 cut(s) 381, 419, 511, 904, 1001, 3184, 3632, 4416, 4834
BslFI GGGAC 5 cut(s) 1740, 2696, 2826, 3582, 4727
BsmBI CGTCTC 3 cut(s) 166, 253, 1524
BsmFI GGGAC 5 cut(s) 1740, 2696, 2826, 3582, 4727
Bso31I GGTCTC 1 cut(s) 4843
BsoBI CYCGRG 2 cut(s) 2157, 2541
Bsp1286I GDGCHC 7 cut(s) 213, 417, 1100, 1130, 1325, 1903, 4009
Bsp13I TCCGGA 1 cut(s) 3183
Bsp1407I TGTACA 4 cut(s) 626, 956, 1079, 3850
Bsp1720I GCTNAGC 1 cut(s) 2679
Bsp19I CCATGG 2 cut(s) 1112, 4853
BspCNI CTCAG 4 cut(s) 191, 957, 3613, 4431
BspEI TCCGGA 1 cut(s) 3183
BspFNI CGCG 6 cut(s) 34, 215, 343, 360, 371, 4369
BspHI TCATGA 2 cut(s) 2113, 2758
BspMAI CTGCAG 7 cut(s) 72, 796, 1054, 1245, 1492, 1574, 4873
BspMI ACCTGC 2 cut(s) 785, 3888
BspOI GCTAGC 1 cut(s) 1286
BspQI GCTCTTC 1 cut(s) 1563
BspT107I GGYRCC 3 cut(s) 150, 513, 3688
BspTI CTTAAG 2 cut(s) 745, 2828
BspTNI GGTCTC 1 cut(s) 4843
BsrDI GCAATG 3 cut(s) 721, 762, 2513
BsrFI RCCGGY 2 cut(s) 510, 1000
BsrGI TGTACA 4 cut(s) 626, 956, 1079, 3850
BsrI ACTGG 8 cut(s) 1105, 2069, 2319, 3011, 3389, 3767, 3812, 4535
BssAI RCCGGY 2 cut(s) 510, 1000
BssNAI GTATAC 2 cut(s) 1890, 3017
BssNI GRCGYC 2 cut(s) 243, 1964
BssSI CACGAG 2 cut(s) 768, 2766
BssT1I CCWWGG 7 cut(s) 146, 1112, 1208, 2849, 3140, 3227, 4853
Bst1107I GTATAC 2 cut(s) 1890, 3017
Bst2BI CACGAG 2 cut(s) 768, 2766
Bst2UI CCWGG 9 cut(s) 1066, 2937, 3066, 3299, 3693, 3723, 3785, 4083, 4191
Bst4CI ACNGT 8 cut(s) 518, 1508, 1942, 2504, 2893, 3640, 3815, 4752
Bst6I CTCTTC 6 cut(s) 42, 431, 641, 1563, 2122, 4503
BstACI GRCGYC 2 cut(s) 243, 1964
BstAFI CTTAAG 2 cut(s) 745, 2828
BstAPI GCANNNNNTGC 3 cut(s) 96, 663, 2417
BstAUI TGTACA 4 cut(s) 626, 956, 1079, 3850
BstBAI YACGTR 3 cut(s) 2435, 2876, 2924
BstDEI CTNAG 7 cut(s) 178, 965, 2465, 2679, 2773, 3600, 4439
BstDSI CCRYGG 6 cut(s) 79, 358, 1112, 1316, 4748, 4853
BstENI CCTNNNNNAGG 1 cut(s) 2567
BstFNI CGCG 6 cut(s) 34, 215, 343, 360, 371, 4369
BstH2I RGCGCY 2 cut(s) 191, 508
BstHHI GCGC 5 cut(s) 190, 217, 345, 507, 2083
BstMCI CGRYCG 2 cut(s) 3215, 3773
BstNI CCWGG 9 cut(s) 1066, 2937, 3066, 3299, 3693, 3723, 3785, 4083, 4191
BstNSI RCATGY 2 cut(s) 2822, 4778
BstPAI GACNNNNGTC 1 cut(s) 1441
BstSFI CTRYAG 9 cut(s) 68, 792, 1050, 1241, 1488, 1570, 1927, 4410, 4869
BstSLI GKGCMC 3 cut(s) 417, 1100, 1903
BstUI CGCG 6 cut(s) 34, 215, 343, 360, 371, 4369
BstV2I GAAGAC 1 cut(s) 2565
BstXI CCANNNNNNTGG 5 cut(s) 993, 3141, 3263, 3330, 4089
BstZ17I GTATAC 2 cut(s) 1890, 3017
BsuI GTATCC 1 cut(s) 2927
BtgI CCRYGG 6 cut(s) 79, 358, 1112, 1316, 4748, 4853
BtgZI GCGATG 1 cut(s) 321
BtrI CACGTC 2 cut(s) 160, 1577
BtsI GCAGTG 4 cut(s) 77, 1497, 1967, 2076
BtsIMutI CAGTG 9 cut(s) 77, 1013, 1098, 1497, 1513, 1967, 2076, 2312, 2509
BveI ACCTGC 2 cut(s) 785, 3888
CciI TCATGA 2 cut(s) 2113, 2758
CfoI GCGC 5 cut(s) 190, 217, 345, 507, 2083
Cfr10I RCCGGY 2 cut(s) 510, 1000
Cfr13I GGNCC 7 cut(s) 392, 709, 937, 1754, 4187, 4289, 4698
Cfr42I CCGCGG 1 cut(s) 361
CseI GACGC 4 cut(s) 40, 232, 360, 1972
CspCI CAANNNNNGTGG 6 cut(s) 1956, 1991, 4237, 4262, 4272, 4297
DdeI CTNAG 7 cut(s) 178, 965, 2465, 2679, 2773, 3600, 4439
DraI TTTAAA 3 cut(s) 816, 2638, 3678
DraIII CACNNNGTG 1 cut(s) 663
DriI GACNNNNNGTC 2 cut(s) 100, 2819
EaeI YGGCCR 7 cut(s) 76, 1546, 1786, 2091, 2316, 3949, 4330
Eam1104I CTCTTC 6 cut(s) 42, 431, 641, 1563, 2122, 4503
Eam1105I GACNNNNNGTC 2 cut(s) 100, 2819
EarI CTCTTC 6 cut(s) 42, 431, 641, 1563, 2122, 4503
EciI GGCGGA 3 cut(s) 247, 563, 1286
Eco130I CCWWGG 7 cut(s) 146, 1112, 1208, 2849, 3140, 3227, 4853
Eco147I AGGCCT 1 cut(s) 338
Eco24I GRGCYC 2 cut(s) 1325, 4009
Eco31I GGTCTC 1 cut(s) 4843
Eco47I GGWCC 3 cut(s) 709, 937, 1754
Eco57I CTGAAG 3 cut(s) 2577, 3490, 4687
Eco72I CACGTG 1 cut(s) 2876
Eco88I CYCGRG 2 cut(s) 2157, 2541
EcoNI CCTNNNNNAGG 1 cut(s) 2567
EcoO109I RGGNCCY 1 cut(s) 4698
EcoRI GAATTC 1 cut(s) 2990
EcoRII CCWGG 9 cut(s) 1064, 2935, 3064, 3297, 3691, 3721, 3783, 4081, 4189
EcoT14I CCWWGG 7 cut(s) 146, 1112, 1208, 2849, 3140, 3227, 4853
EcoT38I GRGCYC 2 cut(s) 1325, 4009
ErhI CCWWGG 7 cut(s) 146, 1112, 1208, 2849, 3140, 3227, 4853
Esp3I CGTCTC 3 cut(s) 166, 253, 1524
FalI AAGNNNNNCTT 4 cut(s) 4482, 4514, 4500, 4532
FaqI GGGAC 5 cut(s) 1740, 2696, 2826, 3582, 4727
FauI CCCGC 4 cut(s) 1429, 1734, 2251, 4259
FauNDI CATATG 1 cut(s) 2662
FblI GTMKAC 2 cut(s) 1889, 3016
FriOI GRGCYC 2 cut(s) 1325, 4009
GlaI GCGC 5 cut(s) 189, 216, 344, 506, 2082
GsaI CCCAGC 2 cut(s) 465, 3569
GsuI CTGGAG 9 cut(s) 211, 969, 1087, 1842, 2379, 2769, 2929, 2958, 3705
HaeII RGCGCY 2 cut(s) 191, 508
HapII CCGG 9 cut(s) 381, 419, 511, 904, 1001, 3184, 3632, 4416, 4834
HgaI GACGC 4 cut(s) 40, 232, 360, 1972
HhaI GCGC 5 cut(s) 190, 217, 345, 507, 2083
Hin1I GRCGYC 2 cut(s) 243, 1964
Hin6I GCGC 5 cut(s) 188, 215, 343, 505, 2081
HinP1I GCGC 5 cut(s) 188, 215, 343, 505, 2081
HindIII AAGCTT 3 cut(s) 1357, 3399, 4467
HpaII CCGG 9 cut(s) 381, 419, 511, 904, 1001, 3184, 3632, 4416, 4834
Hpy99I CGWCG 5 cut(s) 170, 173, 248, 299, 326
HpyCH4III ACNGT 8 cut(s) 518, 1508, 1942, 2504, 2893, 3640, 3815, 4752
HpyCH4IV ACGT 5 cut(s) 159, 1576, 2434, 2875, 2923
HpyF3I CTNAG 7 cut(s) 178, 965, 2465, 2679, 2773, 3600, 4439
HpySE526I ACGT 5 cut(s) 159, 1576, 2434, 2875, 2923
Hsp92I GRCGYC 2 cut(s) 243, 1964
HspAI GCGC 5 cut(s) 188, 215, 343, 505, 2081
Kpn2I TCCGGA 1 cut(s) 3183
KroI GCCGGC 1 cut(s) 510
KroNI GCCGGC 1 cut(s) 512
KspI CCGCGG 1 cut(s) 361
LguI GCTCTTC 1 cut(s) 1563
MaeII ACGT 5 cut(s) 159, 1576, 2434, 2875, 2923
MfeI CAATTG 1 cut(s) 2052
MhlI GDGCHC 7 cut(s) 213, 417, 1100, 1130, 1325, 1903, 4009
MlsI TGGCCA 5 cut(s) 1548, 1788, 2318, 3951, 4332
MluI ACGCGT 2 cut(s) 32, 369
MluNI TGGCCA 5 cut(s) 1548, 1788, 2318, 3951, 4332
MlyI GAGTC 7 cut(s) 720, 976, 1064, 3019, 3402, 3637, 3732
Mox20I TGGCCA 5 cut(s) 1548, 1788, 2318, 3951, 4332
MroI TCCGGA 1 cut(s) 3183
MroNI GCCGGC 1 cut(s) 510
MroXI GAANNNNTTC 3 cut(s) 1022, 2535, 3930
MscI TGGCCA 5 cut(s) 1548, 1788, 2318, 3951, 4332
MslI CAYNNNNRTG 8 cut(s) 607, 1773, 1977, 2665, 2688, 3258, 3539, 4047
Msp20I TGGCCA 5 cut(s) 1548, 1788, 2318, 3951, 4332
MspA1I CMGCKG 3 cut(s) 61, 360, 2096
MspCI CTTAAG 2 cut(s) 745, 2828
MspI CCGG 9 cut(s) 381, 419, 511, 904, 1001, 3184, 3632, 4416, 4834
MunI CAATTG 1 cut(s) 2052
MvaI CCWGG 9 cut(s) 1066, 2937, 3066, 3299, 3693, 3723, 3785, 4083, 4191
MvnI CGCG 6 cut(s) 34, 215, 343, 360, 371, 4369
NaeI GCCGGC 1 cut(s) 512
NciI CCSGG 2 cut(s) 419, 4417
NcoI CCATGG 2 cut(s) 1112, 4853
NdeI CATATG 1 cut(s) 2662
NgoMIV GCCGGC 1 cut(s) 510
NheI GCTAGC 1 cut(s) 1282
NmeAIII GCCGAG 1 cut(s) 95
NmuCI GTSAC 8 cut(s) 1011, 1499, 2390, 2519, 3191, 3532, 3595, 3758
NspI RCATGY 2 cut(s) 2822, 4778
OliI CACNNNNGTG 3 cut(s) 1977, 3258, 3539
PaeR7I CTCGAG 1 cut(s) 2157
PagI TCATGA 2 cut(s) 2113, 2758
PaqCI CACCTGC 2 cut(s) 785, 3888
PceI AGGCCT 1 cut(s) 338
PciSI GCTCTTC 1 cut(s) 1563
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 303, 330
PdiI GCCGGC 1 cut(s) 512
PdmI GAANNNNTTC 3 cut(s) 1022, 2535, 3930
PfeI GAWTC 6 cut(s) 220, 1018, 1856, 4016, 4260, 4585
PflMI CCANNNNNTGG 8 cut(s) 1113, 1118, 2663, 2855, 2952, 3469, 3611, 4859
PfoI TCCNGGA 2 cut(s) 2935, 3721
Ple19I CGATCG 2 cut(s) 3215, 3773
PleI GAGTC 7 cut(s) 720, 975, 1063, 3019, 3401, 3636, 3732
PmaCI CACGTG 1 cut(s) 2876
PmlI CACGTG 1 cut(s) 2876
PpsI GAGTC 7 cut(s) 720, 975, 1063, 3019, 3401, 3636, 3732
Ppu21I YACGTR 3 cut(s) 2435, 2876, 2924
PshAI GACNNNNGTC 1 cut(s) 1441
PsiI TTATAA 3 cut(s) 2370, 2789, 4031
Psp6I CCWGG 9 cut(s) 1064, 2935, 3064, 3297, 3691, 3721, 3783, 4081, 4189
PspCI CACGTG 1 cut(s) 2876
PspFI CCCAGC 2 cut(s) 461, 3565
PspGI CCWGG 9 cut(s) 1064, 2935, 3064, 3297, 3691, 3721, 3783, 4081, 4189
PspPI GGNCC 7 cut(s) 392, 709, 937, 1754, 4187, 4289, 4698
PstI CTGCAG 7 cut(s) 72, 796, 1054, 1245, 1492, 1574, 4873
PvuI CGATCG 2 cut(s) 3215, 3773
RseI CAYNNNNRTG 8 cut(s) 607, 1773, 1977, 2665, 2688, 3258, 3539, 4047
SacII CCGCGG 1 cut(s) 361
SapI GCTCTTC 1 cut(s) 1563
Sau96I GGNCC 7 cut(s) 392, 709, 937, 1754, 4187, 4289, 4698
SchI GAGTC 7 cut(s) 720, 976, 1064, 3019, 3402, 3637, 3732
SduI GDGCHC 7 cut(s) 213, 417, 1100, 1130, 1325, 1903, 4009
SfcI CTRYAG 9 cut(s) 68, 792, 1050, 1241, 1488, 1570, 1927, 4410, 4869
Sfr274I CTCGAG 1 cut(s) 2157
Sfr303I CCGCGG 1 cut(s) 361
SgrBI CCGCGG 1 cut(s) 361
SinI GGWCC 3 cut(s) 709, 937, 1754
SlaI CTCGAG 1 cut(s) 2157
SmiMI CAYNNNNRTG 8 cut(s) 607, 1773, 1977, 2665, 2688, 3258, 3539, 4047
SmlI CTYRAG 6 cut(s) 745, 931, 2157, 2175, 2828, 3869
SmoI CTYRAG 6 cut(s) 745, 931, 2157, 2175, 2828, 3869
SpeI ACTAGT 2 cut(s) 557, 4034
SseBI AGGCCT 1 cut(s) 338
SspI AATATT 1 cut(s) 2610
StuI AGGCCT 1 cut(s) 338
StyI CCWWGG 7 cut(s) 146, 1112, 1208, 2849, 3140, 3227, 4853
TaaI ACNGT 8 cut(s) 518, 1508, 1942, 2504, 2893, 3640, 3815, 4752
TaiI ACGT 5 cut(s) 162, 1579, 2437, 2878, 2926
TaqI TCGA 5 cut(s) 913, 1162, 2158, 3773, 4592
TaqII GACCGA 1 cut(s) 10
TatI WGTACW 6 cut(s) 626, 956, 1079, 1245, 1861, 3850
TfiI GAWTC 6 cut(s) 220, 1018, 1856, 4016, 4260, 4585
TscAI CASTG 9 cut(s) 77, 1020, 1105, 1497, 1513, 1974, 2083, 2319, 2509
TseFI GTSAC 8 cut(s) 1011, 1499, 2390, 2519, 3191, 3532, 3595, 3758
Tsp45I GTSAC 8 cut(s) 1011, 1499, 2390, 2519, 3191, 3532, 3595, 3758
TspGWI ACGGA 8 cut(s) 154, 305, 1333, 2790, 3394, 3438, 3878, 4171
TspRI CASTG 9 cut(s) 77, 1020, 1105, 1497, 1513, 1974, 2083, 2319, 2509
Van91I CCANNNNNTGG 8 cut(s) 1113, 1118, 2663, 2855, 2952, 3469, 3611, 4859
Vha464I CTTAAG 2 cut(s) 745, 2828
VneI GTGCAC 3 cut(s) 413, 1096, 1899
VpaK11BI GGWCC 3 cut(s) 709, 937, 1754
XagI CCTNNNNNAGG 1 cut(s) 2567
XapI RAATTY 8 cut(s) 811, 1137, 1948, 2589, 2633, 2990, 3309, 3576
XbaI TCTAGA 1 cut(s) 4019
XceI RCATGY 2 cut(s) 2822, 4778
XhoI CTCGAG 1 cut(s) 2157
XmaJI CCTAGG 3 cut(s) 146, 1208, 3227
XmiI GTMKAC 2 cut(s) 1889, 3016
XmnI GAANNNNTTC 3 cut(s) 1022, 2535, 3930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.