Prupe.2G040800_v2.0.a1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0010072
Physical Location & Seq
Reverse (-)
4470653 .. 4483827
13175 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G040800.2

Sequence Viewer

Length: 4866 bp
ATGAAAGTGCCGTGCTGCTCCGTCTGCCAGACGCGCTACGACGAGGAAGAAAGAGTCCCGCTTTTGCTTCAGTGCGGCCATGGCTTCTGCAAGGACTGCTTGTCTCGGATGTTCTCTTCTTGTACCGATACCACCCTCGTCTGCCCCAGGTGCCGCCACGTGTCCGTCGTCGGCAACTCCGTTCAGGCCCTCCGCAAGAACTTCGCCGTGCTCGCTCTGATTCACTCGAGTTCCAATGCCGTCTCGTCTGCCTCCGCCGCCAATTTCGATTGCGACTACACGGACGACGAGGATGGGGACGATGATGACGAGGACGATGGGGACCGGCGATGTAGCCGTGGGTCCCACACCTCGAGCTCGGGCGGGTGCGGGCCGCTGATGGAGCTGGCAGTGCACCAAGACTTGAGATTGGTGCGGCGGATAGGAGAGGGGAGGCAGGCCGGGGTCCAAATGTGGACTGCAGTGATTGGCGGCGGAGGCGGGCGGTGCCGCCACAAGATAGCGGTGAAGAAGGTGGCAGTGGCGGAGGAGACGAGTATGGATTGGGTGATGGGGCAGCTGGAGAATTTGCGGCGGGCATCGATGTGGTGTAGGAATGTGTGCACATTTCATGGGGCTATGAAGAGTGAAGGCACTTTGTGTCTTGTTATGGATAGGTGTTATGGGTCTGTTCAGTCCGAGATGCAGCGAAATGAGGGCAGGCTTACTTTGGAGCAAATCCTAAGATATGGGGCTGACATTGCACGAGGAGTGGCTGAACTCCATGCGGCAGGTGTTGTTTGTATGAATCTGAAACCGTCCAATCTTCTTTTGGATTCAAGTGGTCACGCGGTGGTTTCTGATTATGGAGTTGCTGCAATTCTGAAGAAACCTTCCTGCCGGAAAGCTCGACTGGAGTGTGATACCTCAAGGATCCATTCTTGTATGGAATGTACGATGCTCAGCCCCCACTATGCAGCCCCAGAGGCATGGGAGCCGGTGAAGAAATTATTGAATCCATTTTGGGAGGATGCTATTGGTATTTCTACAGAGTCAGATGCATGGAGTTTTGGTTGTACATTGGTTGAAATGTGCACTGGTTCCATTCCATGGGCTGGTTTAAGCACAGAGGAAATTTATCGGGCTGTAATCAAGGCTCGGAAACTGCCTCCACAATATGCAAGTGTAGTAGGTGTTGGAATACCTAGGGAATTGTGGAAAATGATTGGTGAGTGCCTGCAGTTCAAGGCATCAAAAAGACCGTCCTTTAGTTCAATGTTAGCAACATTTCTCCGTCATTTGCAGGAGATACCACGGAGCCCTCCTGCAAGTCCTGATAATGGTTTAGCTAAATGCTCTGGATCAAATGTGACGGAACCGTCTCCTGTATCCCACTCAGAAGTTTTTCACGCTAACCCCACCCTTCTGCATAGACTTGTGTCCGAAGGGGATGTACACGGTGTTAGAGATCTGCTTGAAAAGGCTGCAGCAGAAAGTGATAACAGCGCAGTATTGTCTTTATTAGAAGCTCAAAATGCTGATGGCCAAACTGCTCTCCATTTGGCCTGTCGACGTGGTAGTGCAGAACTTGTTGATGCCATTTTGGAGCATCGAGAGGCAAATGTGGATGTCTTGGACAAAGATGGGGATCCTCCACTTGTTTTTGCTTTAGTTGCTGGATCCCCAGAATGCGTTCGTGCTCTCATTAACAGAGGTGCTAATGTGAGATCTAGGTTGCGGGAAGGCTTTGGCCCGTCTGTTGCTCATGTCTGTGCCTATCATGGCCAGCCTGATTGTATGCGTGAGTTACTAATGGCTGGGGCAGATCCTAATGCAGTGGATGAGGAAGGTGAATCTGTACTACATAGAGCTGTTGCCAAGAAGTATACAGATTGTGCACTTGTCGTTTTGGAAAATGGGGGCTCTAGGTCAATGTCCGTTTTAAATTCAGAAAAATATACACCATTGCACTTGTGTGTGGCAACATGGAATGTTGCTGTTGTTAGAAGGTGGGTGGAAGTTGCAACACCAGAAGAGATTGCCGATGCAATTGATATACCAAGTTCAGTTGGCACCGCGTTGTGTATGGCAGCTGCTCTAAAGAAAGATCATGAAATTGAAGGAAGAGAAATGGTGCATATTTTGCTTGCTTCTGGAGCAGATCCAACTGCTCAAGATGCCCAGCATGGACGGACAGCTTTGCATACAGCTTCAATGGCCAATGACGTTGAGTTGGTCAAGATTATACTTGATGCTGGAGTTGATGTGAACATTCGGAATGTACAAAATACAATTCCTCTTCATGTAGCATTGGCCAGAGGGGCGAAGTCATGTGTCGGACTGCTTCTATCTTCTGGAGCAAATTATAATTTACAGGATGATGAAGGTGACAATGCTTTCCATATAGCAGCAGATGCAGCAAAAATGATACGTGAAAATCTTGAATGGCTTATTGTTATGCTTAGAAATCCTGATGCTTCTGTTGAAGCCAGAAATCACAGCGGCAAGACATTACGTGACTTTTTGGAGGCCCTTCCCCGGGAATGGATATCTGAAGATCTGATGGAGGCACTTGTAAATCGGGGAGTTTTTCTGTCTCCTACAATATTTGATGTAGGAGACTGGGTGAAATTTAAAAGAAGTATAACAACTCCTACATATGGCTGGCAAGGTGCAAAACATAGAAGTGTTGGGTTTGTGCAAGGCGCCCCAGATAAGGACCATTTGTTAGTATCATTTTGTTCCGGAGAGGTTCGTGTTTTGGCAAATGAAGTTGTAAAAGTTATTCCTTTAGACAGGGGACAGCATGTGCAGCTGAAACCAGATGTCAAAGAGCCCAGGTTTGGGTGGCGTGGGCAGTCACGTGACAGCATTGGTACTGTATTGTGTGTTGATGATGATGGTATCCTACGCGTTGGGTTTCCTGGAGCCTCCAGAGGATGGAAAGCTGATCCTGCGGAGATGGAAAGAGTTGAAGAATTCAAGGTTGGGGACTGGGTTCGTATTCGTCCTACTCTTACAACAGCAAAGCATGGACTGGGATCTGTTACACCTGGGAGCATTGGTATTGTGTATTGTATAAGACCTGATAGTAGTCTATTGCTAGAGCTGAGCTATCTTCCAAGTCCATGGCATTGTGAACCAGAGGAGGTTGAACCTGTAATCCCATTCAGGATTGGTGACAGGGTATGTGTGAAGCGATCTGTTGCAGAACCTAGGTATGCATGGGGTGGTGAGACACATCATAGTGTTGGAAGAATAAGTGAGATAGAGAATGATGGCCTCCTGGTAATTGAAATTCCTAATCGACCAATACCATGGCAGGCTGATCCTTCTGACATGGAGAAGGTGGAAGACTTCAAGGTAGGGGATTGGGTTAGAGTTAAAGCTTCTGTGCCCTCTCCCAAATACGGATGGGAGGATATTACAAGGAACAGTGTTGGGATAATTCATAGCTTGGAGGAAGATGGTGATATGGGTGTCGCCTTCTGCTTCAGGAGCAAGCCTTTTTCTTGTTCTGTCACAGATGTGGAGAAGGTGCCCCCTTTTGAATTAGGGCAAGAGATTCATGTGATGGCATCTATTACTCAGCCACGACTTGGATGGTCAAATGAAAGTGCAGCAACTGTTGGAAAAATAGTTAGAATTGACATGGATGGAGCTTTGAATGTAAAAGTGCCTGGTCGACAGAGCTTGTGGAAAGTTTCTCCTGGAGATGCGGAACGACTCTCTGGATTTGAAGTTGGTGACTGGGTACGTTCTAAACCAAGCCTGGGAACTCGACCAAGTTATGATTGGAACAGCATTGGAAAGGAAAGTTTAGCTGTTGTGCATAGTGTACAAGATACAGGTTATCTGGAGTTAGCTTGTTGTTTTCGTAAAGGCAGGTGGATAACTCATTACACGGATGTTGAAAAGGTTCCATGCTTAAAAATAGGGCAGTATGTTCGGTTCCGGACTGGACTAGTTGAACCAAGATGGGGCTGGAGAGGGGCTCAACCTGATTCTCGAGGCATCATAACTAGCGTTCATGCTGATGGGGAAGTCAGAGTGGCTTTCTCTGGTTTGCCAGGGTTGTGGAGAGGAGATCCTGCGGATCTTGAAATAGAGCAGATTTTTGAAGTTGGAGAGTGGGTGAAATTAAAAGATCATGCAAGTATTTGGAAATCCATTGGGCCGAGCAGTGTCGGAGTGGTGCAAGGATTGGGATATGACGGCGATAAATGGGATGGAACCACTTTTGTTGGATTTTGCGGGGAGCAAGAAAAATGGGTTGGGCCTACCTCTGATCTTGCTAGGGTGAACAGGCTCATGGTTGGCCAGAAGGTTAGGGTTAAACTATCTGTCAAGCAGCCAAGATTTGGGTGGTCAGGCCACAGCCATGCAAGTCTTGGAACTATATCCACAATTGATGCTGATGGCAAGCTGAGAATATACACCCCAGCTGGTTCAAAAGCTTGGATGTTGGATCCATCGGAAGTGGAGCTTGTGGAGGAAGAGGAGCTTCACATTGGAGACTGGGTTAGGGTTAAAGCATCAGTTTCAACTCCAACCCACCAGTGGGGAGAGGTGAGTCGTTCGAGCGTTGGGGTGGTGCACCGCATGGAAAATGAGGAGCTCTGGGTGGCATTTTGCTTCACAGAGAGGTTGTGGCTTTGCAAGGCATCGGAAATAGAACGGGTTAGGCCATTTAAAGTGGGGGACAAAGTGAGAATTAGAGAAGGGCTGGTGAGCCCACGGTGGGGTTGGGGAATGGAGACTCATGCAAGCAAAGGACAGGTAGTGGGGGTGGATGCAAATGGAAAGTTAAGAATTAAGTTCCGGTGGAGAGAAGGGAGGCCATGGATCGGAGATCCTGCTGATGTTGCTCTTGATAAAAGCACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Pkinase zf-C3HC4 zf-RING_2 zf-RING_5

Protein Analysis

1622

Amino Acids

178.22

Weight (kDa)

5.94

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2346
AarI CACCTGC 2 cut(s) 761, 3855
AasI GACNNNNNNGTC 1 cut(s) 1357
Acc36I ACCTGC 2 cut(s) 761, 3855
AccB1I GGYRCC 5 cut(s) 150, 486, 2051, 2684, 3517
AccB7I CCANNNNNTGG 5 cut(s) 1089, 1094, 2919, 3578, 4340
AccI GTMKAC 3 cut(s) 1549, 1865, 3664
AccII CGCG 4 cut(s) 34, 830, 2057, 2892
AccIII TCCGGA 2 cut(s) 2723, 3933
AcoI YGGCCR 6 cut(s) 76, 1522, 1762, 2196, 2292, 4297
AcsI RAATTY 6 cut(s) 565, 1113, 1924, 2609, 2957, 3276
AcuI CTGAAG 4 cut(s) 53, 884, 2553, 3457
AcvI CACGTG 2 cut(s) 160, 2843
AcyI GRCGYC 1 cut(s) 2685
AdeI CACNNNGTG 2 cut(s) 639, 832
AfaI GTAC 9 cut(s) 124, 934, 1057, 1434, 1839, 2262, 2857, 3735, 3819
AflIII ACRYGT 2 cut(s) 159, 2890
AhdI GACNNNNNGTC 1 cut(s) 100
AhlI ACTAGT 1 cut(s) 3943
AjiI CACGTC 1 cut(s) 1553
AjnI CCWGG 9 cut(s) 146, 2816, 2902, 3031, 3264, 3658, 3688, 3750, 4048
AjuI GAANNNNNNNTTGG 4 cut(s) 2949, 2981, 4236, 4268
AleI CACNNNNGTG 2 cut(s) 1953, 3506
AloI GAACNNNNNNTCC 2 cut(s) 3126, 3158
Alw21I GWGCWC 9 cut(s) 213, 359, 396, 605, 1076, 1681, 1879, 4578, 4599
Alw26I GTCTC 9 cut(s) 108, 247, 524, 1365, 2580, 2592, 3209, 4488, 4730
Alw44I GTGCAC 5 cut(s) 392, 601, 1072, 1875, 4574
AlwNI CAGNNNCTG 1 cut(s) 3605
Ama87I CYCGRG 5 cut(s) 226, 352, 358, 2517, 3987
Aor13HI TCCGGA 2 cut(s) 2723, 3933
ApaLI GTGCAC 5 cut(s) 392, 601, 1072, 1875, 4574
ApoI RAATTY 6 cut(s) 565, 1113, 1924, 2609, 2957, 3276
ArsI GACNNNNNNTTYG 2 cut(s) 1226, 1258
Asp700I GAANNNNTTC 3 cut(s) 1383, 1671, 3897
AspA2I CCTAGG 2 cut(s) 1184, 3194
AspLEI GCGC 3 cut(s) 36, 1487, 2687
AsuC2I CCSGG 3 cut(s) 442, 2518, 2519
AvaI CYCGRG 5 cut(s) 226, 352, 358, 2517, 3987
AvaII GGWCC 4 cut(s) 322, 342, 445, 2698
AvrII CCTAGG 2 cut(s) 1184, 3194
BaeGI GKGCMC 7 cut(s) 396, 605, 1076, 1879, 3378, 3522, 4578
BalI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 4299
BamHI GGATCC 4 cut(s) 912, 1627, 1658, 4447
BanI GGYRCC 5 cut(s) 150, 486, 2051, 2684, 3517
BanII GRGCYC 7 cut(s) 359, 1301, 1904, 2817, 3976, 4599, 4715
BarI GAAGNNNNNNTAC 2 cut(s) 1416, 1448
BauI CACGAG 1 cut(s) 744
BbrPI CACGTG 2 cut(s) 160, 2843
BbsI GAAGAC 1 cut(s) 3339
Bbv12I GWGCWC 9 cut(s) 213, 359, 396, 605, 1076, 1681, 1879, 4578, 4599
BceAI ACGGC 4 cut(s) 191, 224, 321, 4210
BcgI CGANNNNNNTGC 4 cut(s) 184, 218, 3908, 3942
BciT130I CCWGG 9 cut(s) 148, 2818, 2904, 3033, 3266, 3660, 3690, 3752, 4050
BciVI GTATCC 2 cut(s) 1378, 2894
BcnI CCSGG 3 cut(s) 442, 2518, 2519
BcoDI GTCTC 9 cut(s) 108, 247, 524, 1365, 2580, 2592, 3209, 4488, 4730
BcuI ACTAGT 1 cut(s) 3943
BfaI CTAG 8 cut(s) 1185, 1710, 1905, 3083, 3195, 3944, 4002, 4275
BfmI CTRYAG 4 cut(s) 459, 1026, 1217, 1464
BfoI RGCGCY 1 cut(s) 2688
BfuAI ACCTGC 2 cut(s) 761, 3855
BfuI GTATCC 2 cut(s) 1378, 2894
BglI GCCNNNNNGGC 2 cut(s) 965, 2300
BglII AGATCT 3 cut(s) 1447, 1706, 2536
BlnI CCTAGG 2 cut(s) 1184, 3194
BlpI GCTNAGC 2 cut(s) 941, 3089
Bme18I GGWCC 4 cut(s) 322, 342, 445, 2698
BmeRI GACNNNNNGTC 1 cut(s) 100
BmeT110I CYCGRG 5 cut(s) 226, 352, 358, 2517, 3987
BmgBI CACGTC 1 cut(s) 1553
BmrI ACTGGG 5 cut(s) 2611, 2983, 3026, 3739, 4507
BmuI ACTGGG 5 cut(s) 2611, 2983, 3026, 3739, 4507
BoxI GACNNNNGTC 1 cut(s) 1417
BpiI GAAGAC 1 cut(s) 3339
BplI GAGNNNNNCTC 2 cut(s) 3958, 3990
Bpu1102I GCTNAGC 2 cut(s) 941, 3089
BpuEI CTTGAG 3 cut(s) 424, 892, 2136
BpuMI CCSGG 3 cut(s) 442, 2518, 2519
Bsa29I ATCGAT 1 cut(s) 581
BsaAI YACGTR 5 cut(s) 160, 2411, 2495, 2843, 4863
BsaBI GATNNNNATC 1 cut(s) 1626
BsaHI GRCGYC 1 cut(s) 2685
BsaWI WCCGGW 3 cut(s) 2723, 3933, 4800
Bse118I RCCGGY 2 cut(s) 324, 976
Bse1I ACTGG 9 cut(s) 897, 1081, 2606, 2978, 3021, 3734, 3943, 4502, 4537
Bse3DI GCAATG 2 cut(s) 738, 1943
Bse8I GATNNNNATC 1 cut(s) 1626
BseAI TCCGGA 2 cut(s) 2723, 3933
BseBI CCWGG 9 cut(s) 148, 2818, 2904, 3033, 3266, 3660, 3690, 3752, 4050
BseCI ATCGAT 1 cut(s) 581
BseJI GATNNNNATC 1 cut(s) 1626
BseMI GCAATG 2 cut(s) 738, 1943
BseMII CTCAG 5 cut(s) 955, 1389, 3080, 3581, 4397
BseNI ACTGG 9 cut(s) 897, 1081, 2606, 2978, 3021, 3734, 3943, 4502, 4537
BseRI GAGGAG 6 cut(s) 542, 762, 3140, 4077, 4493, 4607
BseSI GKGCMC 7 cut(s) 396, 605, 1076, 1879, 3378, 3522, 4578
BseYI CCCAGC 3 cut(s) 1796, 2160, 4420
BsgI GTGCAG 3 cut(s) 1581, 2810, 3618
Bsh1236I CGCG 4 cut(s) 34, 830, 2057, 2892
BshNI GGYRCC 5 cut(s) 150, 486, 2051, 2684, 3517
BshVI ATCGAT 1 cut(s) 581
BsiHKAI GWGCWC 9 cut(s) 213, 359, 396, 605, 1076, 1681, 1879, 4578, 4599
BsiHKCI CYCGRG 5 cut(s) 226, 352, 358, 2517, 3987
BsiSI CCGG 8 cut(s) 325, 441, 880, 977, 2518, 2724, 3934, 4801
BslFI GGGAC 7 cut(s) 41, 311, 328, 335, 2793, 2984, 4694
BsmAI GTCTC 9 cut(s) 108, 247, 524, 1365, 2580, 2592, 3209, 4488, 4730
BsmBI CGTCTC 3 cut(s) 247, 524, 1365
BsmFI GGGAC 7 cut(s) 41, 311, 328, 335, 2793, 2984, 4694
BsmI GAATGC 1 cut(s) 1673
BsoBI CYCGRG 5 cut(s) 226, 352, 358, 2517, 3987
Bsp13I TCCGGA 2 cut(s) 2723, 3933
Bsp1407I TGTACA 4 cut(s) 1055, 1432, 2260, 3817
Bsp1720I GCTNAGC 2 cut(s) 941, 3089
Bsp19I CCATGG 5 cut(s) 79, 1088, 3107, 3296, 4820
BspCNI CTCAG 5 cut(s) 954, 1388, 3081, 3580, 4398
BspDI ATCGAT 1 cut(s) 581
BspEI TCCGGA 2 cut(s) 2723, 3933
BspFNI CGCG 4 cut(s) 34, 830, 2057, 2892
BspHI TCATGA 1 cut(s) 2089
BspMAI CTGCAG 3 cut(s) 463, 1221, 1468
BspMI ACCTGC 2 cut(s) 761, 3855
BspT107I GGYRCC 5 cut(s) 150, 486, 2051, 2684, 3517
BsrDI GCAATG 2 cut(s) 738, 1943
BsrFI RCCGGY 2 cut(s) 324, 976
BsrGI TGTACA 4 cut(s) 1055, 1432, 2260, 3817
BsrI ACTGG 9 cut(s) 897, 1081, 2606, 2978, 3021, 3734, 3943, 4502, 4537
BssAI RCCGGY 2 cut(s) 324, 976
BssNAI GTATAC 1 cut(s) 1866
BssNI GRCGYC 1 cut(s) 2685
BssSI CACGAG 1 cut(s) 744
BssT1I CCWWGG 7 cut(s) 79, 1088, 1184, 3107, 3194, 3296, 4820
Bst1107I GTATAC 1 cut(s) 1866
Bst2BI CACGAG 1 cut(s) 744
Bst2UI CCWGG 9 cut(s) 148, 2818, 2904, 3033, 3266, 3660, 3690, 3752, 4050
Bst4CI ACNGT 8 cut(s) 798, 1242, 1359, 1439, 2860, 3416, 3607, 4719
Bst6I CTCTTC 6 cut(s) 121, 617, 2007, 2098, 2283, 4470
BstACI GRCGYC 1 cut(s) 2685
BstAPI GCANNNNNTGC 3 cut(s) 96, 2122, 2393
BstAUI TGTACA 4 cut(s) 1055, 1432, 2260, 3817
BstBAI YACGTR 5 cut(s) 160, 2411, 2495, 2843, 4863
BstDEI CTNAG 7 cut(s) 722, 941, 1375, 2441, 3089, 3567, 4406
BstDSI CCRYGG 8 cut(s) 79, 337, 1088, 1292, 3107, 3296, 4715, 4820
BstFNI CGCG 4 cut(s) 34, 830, 2057, 2892
BstH2I RGCGCY 1 cut(s) 2688
BstHHI GCGC 3 cut(s) 36, 1487, 2687
BstMAI GTCTC 9 cut(s) 108, 247, 524, 1365, 2580, 2592, 3209, 4488, 4730
BstNI CCWGG 9 cut(s) 148, 2818, 2904, 3033, 3266, 3660, 3690, 3752, 4050
BstNSI RCATGY 1 cut(s) 2789
BstPAI GACNNNNGTC 1 cut(s) 1417
BstSFI CTRYAG 4 cut(s) 459, 1026, 1217, 1464
BstSLI GKGCMC 7 cut(s) 396, 605, 1076, 1879, 3378, 3522, 4578
BstUI CGCG 4 cut(s) 34, 830, 2057, 2892
BstV2I GAAGAC 1 cut(s) 3339
BstXI CCANNNNNNTGG 4 cut(s) 969, 3108, 3297, 4056
BstZ17I GTATAC 1 cut(s) 1866
Bsu15I ATCGAT 1 cut(s) 581
BsuI GTATCC 2 cut(s) 1378, 2894
BsuTUI ATCGAT 1 cut(s) 581
BtgI CCRYGG 8 cut(s) 79, 337, 1088, 1292, 3107, 3296, 4715, 4820
BtgZI GCGATG 1 cut(s) 343
BtrI CACGTC 1 cut(s) 1553
BtsI GCAGTG 5 cut(s) 396, 468, 525, 1821, 4168
BtsIMutI CAGTG 9 cut(s) 77, 396, 468, 525, 1074, 1821, 3421, 4168, 4544
BveI ACCTGC 2 cut(s) 761, 3855
CaiI CAGNNNCTG 1 cut(s) 3605
CciI TCATGA 1 cut(s) 2089
CfoI GCGC 3 cut(s) 36, 1487, 2687
Cfr10I RCCGGY 2 cut(s) 324, 976
Cfr9I CCCGGG 1 cut(s) 2517
ClaI ATCGAT 1 cut(s) 581
CseI GACGC 1 cut(s) 40
Csp6I GTAC 9 cut(s) 123, 933, 1056, 1433, 1838, 2261, 2856, 3734, 3818
CspCI CAANNNNNGTGG 2 cut(s) 4204, 4239
CviQI GTAC 9 cut(s) 123, 933, 1056, 1433, 1838, 2261, 2856, 3734, 3818
DdeI CTNAG 7 cut(s) 722, 941, 1375, 2441, 3089, 3567, 4406
DinI GGCGCC 1 cut(s) 2686
DraI TTTAAA 3 cut(s) 1923, 2614, 4672
DraIII CACNNNGTG 2 cut(s) 639, 832
DrdI GACNNNNNNGTC 1 cut(s) 1357
DriI GACNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 1357
EaeI YGGCCR 6 cut(s) 76, 1522, 1762, 2196, 2292, 4297
Eam1104I CTCTTC 6 cut(s) 121, 617, 2007, 2098, 2283, 4470
Eam1105I GACNNNNNGTC 1 cut(s) 100
EarI CTCTTC 6 cut(s) 121, 617, 2007, 2098, 2283, 4470
EciI GGCGGA 4 cut(s) 244, 433, 489, 539
Ecl136II GAGCTC 2 cut(s) 357, 4597
Eco130I CCWWGG 7 cut(s) 79, 1088, 1184, 3107, 3194, 3296, 4820
Eco24I GRGCYC 7 cut(s) 359, 1301, 1904, 2817, 3976, 4599, 4715
Eco32I GATATC 1 cut(s) 2529
Eco47I GGWCC 4 cut(s) 322, 342, 445, 2698
Eco53kI GAGCTC 2 cut(s) 357, 4597
Eco57I CTGAAG 4 cut(s) 53, 884, 2553, 3457
Eco72I CACGTG 2 cut(s) 160, 2843
Eco88I CYCGRG 5 cut(s) 226, 352, 358, 2517, 3987
EcoICRI GAGCTC 2 cut(s) 357, 4597
EcoO109I RGGNCCY 3 cut(s) 187, 342, 2509
EcoRI GAATTC 1 cut(s) 2957
EcoRII CCWGG 9 cut(s) 146, 2816, 2902, 3031, 3264, 3658, 3688, 3750, 4048
EcoRV GATATC 1 cut(s) 2529
EcoT14I CCWWGG 7 cut(s) 79, 1088, 1184, 3107, 3194, 3296, 4820
EcoT22I ATGCAT 2 cut(s) 1042, 3205
EcoT38I GRGCYC 7 cut(s) 359, 1301, 1904, 2817, 3976, 4599, 4715
EgeI GGCGCC 1 cut(s) 2686
EheI GGCGCC 1 cut(s) 2686
ErhI CCWWGG 7 cut(s) 79, 1088, 1184, 3107, 3194, 3296, 4820
Esp3I CGTCTC 3 cut(s) 247, 524, 1365
FalI AAGNNNNNCTT 6 cut(s) 83, 115, 4449, 4481, 4467, 4499
FaqI GGGAC 7 cut(s) 41, 311, 328, 335, 2793, 2984, 4694
FauI CCCGC 7 cut(s) 66, 356, 362, 473, 567, 1710, 4226
FauNDI CATATG 1 cut(s) 2638
FblI GTMKAC 3 cut(s) 1549, 1865, 3664
FriOI GRGCYC 7 cut(s) 359, 1301, 1904, 2817, 3976, 4599, 4715
FspBI CTAG 8 cut(s) 1185, 1710, 1905, 3083, 3195, 3944, 4002, 4275
GlaI GCGC 3 cut(s) 35, 1486, 2686
GsaI CCCAGC 3 cut(s) 1800, 2164, 4424
HaeII RGCGCY 1 cut(s) 2688
HapII CCGG 8 cut(s) 325, 441, 880, 977, 2518, 2724, 3934, 4801
HgaI GACGC 1 cut(s) 40
HhaI GCGC 3 cut(s) 36, 1487, 2687
Hin1I GRCGYC 1 cut(s) 2685
Hin6I GCGC 3 cut(s) 34, 1485, 2685
HinP1I GCGC 3 cut(s) 34, 1485, 2685
HincII GTYRAC 2 cut(s) 1550, 3665
HindII GTYRAC 2 cut(s) 1550, 3665
HindIII AAGCTT 2 cut(s) 3366, 4434
HpaII CCGG 8 cut(s) 325, 441, 880, 977, 2518, 2724, 3934, 4801
Hpy99I CGWCG 5 cut(s) 44, 170, 173, 290, 1554
HpyCH4III ACNGT 8 cut(s) 798, 1242, 1359, 1439, 2860, 3416, 3607, 4719
HpyCH4IV ACGT 8 cut(s) 159, 1552, 2205, 2410, 2494, 2842, 3736, 4862
HpyF3I CTNAG 7 cut(s) 722, 941, 1375, 2441, 3089, 3567, 4406
HpySE526I ACGT 8 cut(s) 159, 1552, 2205, 2410, 2494, 2842, 3736, 4862
Hsp92I GRCGYC 1 cut(s) 2685
HspAI GCGC 3 cut(s) 34, 1485, 2685
KasI GGCGCC 1 cut(s) 2684
KflI GGGWCCC 1 cut(s) 342
Kpn2I TCCGGA 2 cut(s) 2723, 3933
MaeI CTAG 8 cut(s) 1185, 1710, 1905, 3083, 3195, 3944, 4002, 4275
MaeII ACGT 8 cut(s) 159, 1552, 2205, 2410, 2494, 2842, 3736, 4862
MfeI CAATTG 2 cut(s) 2028, 4386
MlsI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 4299
MluI ACGCGT 1 cut(s) 2890
MluNI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 4299
Mly113I GGCGCC 1 cut(s) 2685
MlyI GAGTC 5 cut(s) 63, 1040, 3699, 4561, 4732
Mox20I TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 4299
Mph1103I ATGCAT 2 cut(s) 1042, 3205
MroI TCCGGA 2 cut(s) 2723, 3933
MroXI GAANNNNTTC 3 cut(s) 1383, 1671, 3897
MscI TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 4299
MslI CAYNNNNRTG 8 cut(s) 583, 1749, 1953, 2664, 3225, 3506, 4014, 4359
Msp20I TGGCCA 5 cut(s) 1524, 1764, 2198, 2294, 4299
MspA1I CMGCKG 6 cut(s) 376, 559, 2072, 2481, 2794, 4424
MspI CCGG 8 cut(s) 325, 441, 880, 977, 2518, 2724, 3934, 4801
MunI CAATTG 2 cut(s) 2028, 4386
Mva1269I GAATGC 1 cut(s) 1673
MvaI CCWGG 9 cut(s) 148, 2818, 2904, 3033, 3266, 3660, 3690, 3752, 4050
MvnI CGCG 4 cut(s) 34, 830, 2057, 2892
NarI GGCGCC 1 cut(s) 2685
NciI CCSGG 3 cut(s) 442, 2518, 2519
NcoI CCATGG 5 cut(s) 79, 1088, 3107, 3296, 4820
NdeI CATATG 1 cut(s) 2638
NmeAIII GCCGAG 1 cut(s) 4182
NmuCI GTSAC 9 cut(s) 824, 1348, 2366, 2495, 2838, 2843, 3158, 3499, 3725
NsiI ATGCAT 2 cut(s) 1042, 3205
NspI RCATGY 1 cut(s) 2789
OliI CACNNNNGTG 2 cut(s) 1953, 3506
PaeR7I CTCGAG 3 cut(s) 226, 352, 3987
PagI TCATGA 1 cut(s) 2089
PaqCI CACCTGC 2 cut(s) 761, 3855
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 177, 306
PctI GAATGC 1 cut(s) 1673
PdmI GAANNNNTTC 3 cut(s) 1383, 1671, 3897
PfeI GAWTC 7 cut(s) 220, 787, 815, 994, 1832, 3544, 3983
PflMI CCANNNNNTGG 5 cut(s) 1089, 1094, 2919, 3578, 4340
PfoI TCCNGGA 2 cut(s) 2902, 3688
PleI GAGTC 5 cut(s) 62, 1039, 3699, 4560, 4732
PluTI GGCGCC 1 cut(s) 2688
PmaCI CACGTG 2 cut(s) 160, 2843
PmlI CACGTG 2 cut(s) 160, 2843
PpsI GAGTC 5 cut(s) 62, 1039, 3699, 4560, 4732
Ppu21I YACGTR 5 cut(s) 160, 2411, 2495, 2843, 4863
PpuMI RGGWCCY 1 cut(s) 342
PshAI GACNNNNGTC 1 cut(s) 1417
PsiI TTATAA 1 cut(s) 2346
Psp124BI GAGCTC 2 cut(s) 359, 4599
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 9 cut(s) 146, 2816, 2902, 3031, 3264, 3658, 3688, 3750, 4048
PspCI CACGTG 2 cut(s) 160, 2843
PspFI CCCAGC 3 cut(s) 1796, 2160, 4420
PspGI CCWGG 9 cut(s) 146, 2816, 2902, 3031, 3264, 3658, 3688, 3750, 4048
PspPPI RGGWCCY 1 cut(s) 342
PspXI VCTCGAGB 2 cut(s) 226, 352
PsrI GAACNNNNNNTAC 2 cut(s) 3914, 3946
PstI CTGCAG 3 cut(s) 463, 1221, 1468
PstNI CAGNNNCTG 1 cut(s) 3605
PvuII CAGCTG 4 cut(s) 559, 2072, 2794, 4424
RsaI GTAC 9 cut(s) 124, 934, 1057, 1434, 1839, 2262, 2857, 3735, 3819
RsaNI GTAC 9 cut(s) 123, 933, 1056, 1433, 1838, 2261, 2856, 3734, 3818
RseI CAYNNNNRTG 8 cut(s) 583, 1749, 1953, 2664, 3225, 3506, 4014, 4359
SacI GAGCTC 2 cut(s) 359, 4599
SalI GTCGAC 2 cut(s) 1548, 3663
SchI GAGTC 5 cut(s) 63, 1040, 3699, 4561, 4732
SfcI CTRYAG 4 cut(s) 459, 1026, 1217, 1464
SfoI GGCGCC 1 cut(s) 2686
Sfr274I CTCGAG 3 cut(s) 226, 352, 3987
SinI GGWCC 4 cut(s) 322, 342, 445, 2698
SlaI CTCGAG 3 cut(s) 226, 352, 3987
SmaI CCCGGG 1 cut(s) 2519
SmiMI CAYNNNNRTG 8 cut(s) 583, 1749, 1953, 2664, 3225, 3506, 4014, 4359
SmlI CTYRAG 6 cut(s) 226, 352, 403, 907, 2151, 3987
SmoI CTYRAG 6 cut(s) 226, 352, 403, 907, 2151, 3987
SpeI ACTAGT 1 cut(s) 3943
SspDI GGCGCC 1 cut(s) 2684
SspI AATATT 1 cut(s) 2586
SspMI CTAG 8 cut(s) 1185, 1710, 1905, 3083, 3195, 3944, 4002, 4275
SstI GAGCTC 2 cut(s) 359, 4599
StyI CCWWGG 7 cut(s) 79, 1088, 1184, 3107, 3194, 3296, 4820
TaaI ACNGT 8 cut(s) 798, 1242, 1359, 1439, 2860, 3416, 3607, 4719
TaiI ACGT 8 cut(s) 162, 1555, 2208, 2413, 2497, 2845, 3739, 4865
TatI WGTACW 5 cut(s) 1055, 1432, 1837, 2260, 3817
TfiI GAWTC 7 cut(s) 220, 787, 815, 994, 1832, 3544, 3983
TscAI CASTG 9 cut(s) 77, 396, 468, 525, 1081, 1821, 3421, 4168, 4544
TseFI GTSAC 9 cut(s) 824, 1348, 2366, 2495, 2838, 2843, 3158, 3499, 3725
Tsp45I GTSAC 9 cut(s) 824, 1348, 2366, 2495, 2838, 2843, 3158, 3499, 3725
TspMI CCCGGG 1 cut(s) 2517
TspRI CASTG 9 cut(s) 77, 396, 468, 525, 1081, 1821, 3421, 4168, 4544
Van91I CCANNNNNTGG 5 cut(s) 1089, 1094, 2919, 3578, 4340
VneI GTGCAC 5 cut(s) 392, 601, 1072, 1875, 4574
VpaK11BI GGWCC 4 cut(s) 322, 342, 445, 2698
XapI RAATTY 6 cut(s) 565, 1113, 1924, 2609, 2957, 3276
XceI RCATGY 1 cut(s) 2789
XcmI CCANNNNNNNNNTGG 8 cut(s) 808, 3579, 3771, 3960, 4341, 4367, 4536, 4722
XhoI CTCGAG 3 cut(s) 226, 352, 3987
XmaI CCCGGG 1 cut(s) 2517
XmaJI CCTAGG 2 cut(s) 1184, 3194
XmiI GTMKAC 3 cut(s) 1549, 1865, 3664
XmnI GAANNNNTTC 3 cut(s) 1383, 1671, 3897
XspI CTAG 8 cut(s) 1185, 1710, 1905, 3083, 3195, 3944, 4002, 4275
Zsp2I ATGCAT 2 cut(s) 1042, 3205
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.