Rroxscaffold_4G00324310

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0010072
Physical Location & Seq
Forward (+)
55700011 .. 55714114
14104 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00324310.1

Sequence Viewer

Length: 4776 bp
ATGTTCTCCGCCTGTCCCGACACCACGCTCGTCTGCCCTAGGTGCCGCCACGTCTCCGTCGTCGGCAACTCCGTTCAGGCTCTCCGGAAAAACTACGCCGTTCTGGCGCTGATTCACTCCAGCACCGCCTCCAATGGTGTCTCCTCCGCCGCCGCCGCCGCCGCCGCAAATTTCGATTGCGATTATACGGACGATGAGGGCGGCGATGAGGATGAGGATGATGATAACGACGAGCCCTCGCTGTCGCGGCGCCGATGTAGCCGCGGATCAAACGCGTCCAGCTCCGGCGGGTGTGGGCCGGTCATTGAGGTGGCGGTGCACCCCGAGTTGAGGTTTGTGAGGCGGATAGGGGAGGGCAGGCAGGCTGGGGTTCAAATGTGGACGGCAGTGATCGGCGGGAGTGGGTGGCGGTGCCGGCACCGTGTGGCGGTGAAGAAGGTGGCAGTGGTGGCGGAGGAGACGAGTATGGATTGGGTGATGGGGCAACTGGAGAACTTGCGGCGGGCGTCCATGTGGTGTAGGAATGTGTGCACATTTCATGGGGCGGTGAAGAATGAAGGGACTCTGTGTCTTGTTATGGATAAGTGTTTTGGGTCTGTTCAATCCGAGATGGAGCGCAATGAGGGCAGACTCACTCTGGAGCAAATCTTAAGATATGGTGCTGACATTGCACGAGGAGTAGCTGAACTCCATGCCGCAGGTGTTGTTTGTATGAATCTAAAACCATCCAATTTACTTTTGGATGCGAATGGTCATGCTGTGGTTTCTGATTATGGAGTTGCTGCTATTCTTAAGAAACCTTCCTGTCGCAAAACTCGATCAGAATGTGACACCTCAAGGAGCCATTCGTGTATGGAGTGTACAATGCTAAGTCCTCACTATGCAGCACCAGAGGCATGGGAGCCGGTGAAGAAGTTACTGAATCCATTCTGGGATGAAGCTATTGGTATCTCTACAGAGTCAGATGCATGGAGTTTTGGTTGTACATTGGTAGAAATGTGCACCGGTTCCATCCCATGGGCTGGTTTGAGCACAGAGGAAATTTACAAGGCAGTGGTCAAGGCTCGAAAACTGCCTCCACAATATGCAAGTGTAGTAGGTGTTGGAATACCTAGGGAATTGTGGAAAATGATTGGTGAGTGCCTGCAGTACAAGGCATCAAAAAGACCATCCTTTAATTTAATGCTAGCAACTTTCCTCCGCCATTTGCAGGAGATACCGCGGAGCCCTCCTGCTAGTCCTGATAATGACGTAGCTAAAAGCTTGGGATCCAATGTGAAGGAACCATCGCCCGTATCCTATTCAAAAGTATTTCAGGGTAATCCCGCCCTTCTGCATAGACTTGTGTCCGAAGGGGATGTATATGGTGTTAGATTATACAGAGATCTGCTTGGAAAGGCTGCAGCGGGAAGTGACAACAGTGTAATATCGTCTCTATTAGAAGCTCAAAATGCTGATGGCCAAACTGCTCTTCATTTGGCCTGTAGACGTGGTAGTGCAGAACTTGTTGATGCTATTTTGGAGTATAGAGAGGCAAATGTAGATGTGTTGGACAAAGATGGGGATCCTCCACTTGTTTTTGCTCTAGTAGCTGGATCCCCAGAGTGTGTTCATGTTCTCATTAAAAGAGGTGCTAATGTGAGATCTCGGTTGCGGGAGGGCTTTGGTCCCTCTGTTGCTCATGTCTGTGCTTATCACGGCCAGCCTGATTGTATGCGTGAGTTACTAATGGCTGGAGCTGATCCTAATGCGGTGGATGAGGAAGGGGAATCTGTACTACATAGAGCTATTACCAAGAAGTATACAGATTGTGCACTTGTGGTTTTGGAAAATGGGGGCTGTAGGTCAATGACTGTTCTAAATTCAGAAAAAATGACGCCATTGCACTTGTGTGTGCAAACATGGAATGTTGCTGTTGTTAGAAGATGGGTGGAAGTTGCAACACCAGAAGAGATTGCCGACGCAATTGATATACCAAGTCCAGTTGGCACTGCGCTGTGTATGGCTGCTGCTTTAAAGAAAGATCATGAAATTGGTAATCCTAGATTGTATGGTTTGGCAGGAGTCCCTGGATTTGAGCCTGGGTCCTCAATGCGGGTTGGAGGAGTTCTGGAAGGAAGAGAATTGGTGCATATTTTGCTTGCTTCTGGAGCAGATCCAACTGCTCAGGATGCCCAGCATGGACGTACAGCTTTGCATACGGCTTCAATGGCCAATGACGTTGAGTTGGTGAAGATTATACTTGATGCTGGAGTTGATGTGAACATCCGAAATGCACAAAATACAATTCCACTTCATGTAGCACTGGCCAGAGGGGCGAAGTCATGTGTTGGGATGCTTCTATCCGCTGGAGCAAATTATAATTTGCAGGATGATGAGGGGGACAATGCTTTCCATATAGCAGCAGATGCAGCAAAAATGATACGTGAAAATCTTGAATGGCTTATTGTCATGCTTAGAAATCCTGATGCTTCTGTTGAAGCCAGAAATCACAGGCAATTCCACAGTGGCAAGACATTGCGTGACTTTCTGGAAGCTCTTCCCCGGGAATGGGTATCTGAAGACCTGATGGAGGCACTTATTAATAGGGGAGTTTATCTGTCTCCTACAATTGGGGATTGGATCAAATTTAAAAGAAGTATCACAAATCCGACATATGGATGGCAAGGTGCAAAGCATAGAAGTGTAGGCTTCGTGCAAAGTGTCCCAGATAAGGACAATCTGATAGTATCATTTTGTTCTGGAGAGGCTCATGAGGCTCGTGTCTTAGCAAATGAAGTTATAAAGGTTATTCCGTTAGACAGGGGGCAGCACGTCCAGCTTAAACCCGATGTTAAAGAGCCAAGGTTTGGGTGGCGTGGGCAATCACGTGATAGCATTGGTACTGTATTATGTGTTGATGATGATGGTATCCTACGTGTTGGGTTTCCTGGAGCCTCCAGAGGCTGGAAAGCTGATCCTGCAGAAATGGAGAGAGTTGAAGAATTCAAGGTTGGGGACTGGGTTCGTATTCGCCCGACTCTTACAACAGCAAAGCATGGATTAGGATCTGTCACACCTGGGAGCATTGGTATTGTGTATTGTATACGACCAGATAGTAGTCTGTTGTTAGAATTAAGTTATCTTCCAAATCCATGGCATTGTGAACCGGAGGAGGTTGAGCCTGTAATCCCCTTCAGGATTGGTGACAGGGTATGTGTGAAGCGATCAGTTGCAGAACCTAGGTATGCATGGGGTGGTGAGACCCACCATAGTGTTGGAAGAATAAGTGAGATAGAGAATGATGGCCTCCTGGTTATTGAAATTCCAAACCGACCAATATCATGGCAGGCTGATCCTTCTGACATGGAGAAGTTGGAAGATTTCAAGGTAGGAGATTGGGTTAGAGTCAAAGCTTCAGTACCCTCCCCCAAATACGGATGGGAGGATATCACAAGGAGTAGTATTGGGATAATCCATAGCTTGGAGGAAGATGGTGATATGGGTGTTGCTTTTTGCTTCAGGAGCAAGCCTTTTTCTTGTTCTGTCACGGACGTGGAAAAGCTACCTCCTTTTGAGGTAAGGGTGCCTGGCAGACAGAGCTTGTGGAAAGTTTCTCCAGGAGATGCTGAACGACTCTCTGGATTTGAAGTTGGTGACTGGGTACGATCAAAACCAAGCCTGGGAACTCGACCAAGTTATGACTGGAACAGTATTGGAAAAGAAAGTTTAGCTGTTGTTCACAGTGTACAAGACACAGGTTATCTTGAGTTGGCTTGCTGTTTCCGTAAAGGCAGGTGGATAACTCATTATACAGACGTTGAAAAGGTTCCATGCTTCAAAGTTGGCCAATATGTTCGGTTTCGTACTGGTTTAGTTGAACCAAGATGGGGATGGAGAGGGGCTCAACCTGATTCTCGAGGTATTATAACTAGTATTCATGCTGATGGGGAAGTGAGAGTGGCTTTCCCTGGTTTGCTAGGGTTGTGGAGAGGAGATCCTGCTGATTTTGAGATAGAGCAGATTTATGAAGTTGGAGAGTGGGTGAGATTAGAAGATCATGCAAATATGTGGAAATCTGTTGGGCCAGGGAGTGTTGGCGTGGTGCAAGGATTAGGATACGAGGGAGATAAATGGGATGGAACCACTTTTGTAGGATTCTGCGGGGAGCAAGAGAGATGGATTGGGCCTACTTCTGATCTTGCAAGGGCAAACAGACTCTTGGTTGGCCAGAAGGTTAGGGTTAAATTATCTGTCAAGCAGCCCCGATTTGGATGGTCAGGCCACAGCCATGCAAGTGTTGGAACTATAGCTGGGATTGATGCTGATGGAAAGCTGAGAATATACACTCCAACTGGTTCAAAAGCTTGGATGTTGGATCCATCAGAAGTGCAACTTGTGGAGGAAGAGGAGCTTCATATTGGAGACTGGGTTAGGGTTAAAACATCAATATCAACTCCAACCCACCAATGGGGAGAGGTGAATCGTTCGAGCGTTGGGGTGGTGCATCGGATGGAAAATGAGGAGCTTTGGGTGGCTTTTTGCTTCACGGAGAGGTTGTGGCTTTGCAAGGCATTGGAAATGGAACGGGTCAGGCCCTTTAGAGTGGGGGACAAAGTGAGGATTAGAGAAGGGTTGGTGTCCCCACGGTGGGGTTGGGGAATGGAGACGCATGCCAGCAAAGGAGAGGTAGTTGGGGTGGATGCAAATGGAAAGTTAAGAATTAAGTTCCGGTGGAGAGAAGGGAGACCATGGATTGGAGATCCTGCTGATGTCACTCTTGATGAAAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Pkinase zf-C3HC4 zf-RING_2 zf-RING_5

Protein Analysis

1591

Amino Acids

175.16

Weight (kDa)

5.97

Isoelectric Point (pI)

41.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 111 - 396 4.2e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 137 - 396 1.3e-29 Protein kinase domain
Ank_2 PF12796 467 - 549 1.3e-12 Ankyrin repeats (3 copies)
Ank PF00023 486 - 519 6.3e-06 Ankyrin repeat
Ank_2 PF12796 561 - 641 9.1e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 561 - 605 5.4e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 707 - 791 1.1e-13 Ankyrin repeats (3 copies)
Ank PF00023 727 - 758 1.9e-06 Ankyrin repeat
Ank_4 PF13637 732 - 781 3.6e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 740 - 804 2e-07 Ankyrin repeats (3 copies)
SH3_15 PF18346 925 - 990 9e-14 Mind bomb SH3 repeat domain
SH3_15 PF18346 1059 - 1116 3.2e-08 Mind bomb SH3 repeat domain
SH3_15 PF18346 1122 - 1169 6e-06 Mind bomb SH3 repeat domain
SH3_15 PF18346 1209 - 1329 1.2e-15 Mind bomb SH3 repeat domain
SH3_15 PF18346 1336 - 1457 2.5e-16 Mind bomb SH3 repeat domain
SH3_15 PF18346 1466 - 1585 1.7e-14 Mind bomb SH3 repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2361, 2783, 3908
AarI CACCTGC 2 cut(s) 689, 3765
Acc36I ACCTGC 2 cut(s) 689, 3765
AccB1I GGYRCC 5 cut(s) 42, 249, 411, 417, 3565
AccB7I CCANNNNNTGG 6 cut(s) 1017, 1022, 2849, 2946, 3463, 4736
AccI GTMKAC 3 cut(s) 1486, 1802, 3085
AccII CGCG 4 cut(s) 247, 264, 275, 1222
AccIII TCCGGA 1 cut(s) 84
AcoI YGGCCR 6 cut(s) 1459, 1699, 2211, 2307, 3826, 4207
AcsI RAATTY 6 cut(s) 169, 1041, 1861, 2627, 2984, 3303
AcuI CTGAAG 4 cut(s) 2580, 3160, 3381, 3484
AcvI CACGTG 1 cut(s) 2870
AcyI GRCGYC 3 cut(s) 250, 506, 1877
AdeI CACNNNGTG 1 cut(s) 424
AflII CTTAAG 2 cut(s) 649, 791
AflIII ACRYGT 2 cut(s) 273, 2917
AgeI ACCGGT 1 cut(s) 1004
AhdI GACNNNNNGTC 1 cut(s) 567
AhlI ACTAGT 1 cut(s) 3911
AjiI CACGTC 4 cut(s) 52, 1490, 2815, 3535
AjuI GAANNNNNNNTTGG 4 cut(s) 1455, 1487, 2976, 3008
AleI CACNNNNGTG 3 cut(s) 1890, 3252, 3533
Alw21I GWGCWC 5 cut(s) 321, 533, 1004, 1034, 1816
Alw26I GTCTC 9 cut(s) 58, 145, 452, 1437, 2607, 3236, 4398, 4640, 4720
Alw44I GTGCAC 4 cut(s) 317, 529, 1000, 1812
AlwNI CAGNNNCTG 1 cut(s) 2946
Ama87I CYCGRG 3 cut(s) 323, 2544, 3897
Aor13HI TCCGGA 1 cut(s) 84
ApaLI GTGCAC 4 cut(s) 317, 529, 1000, 1812
ApoI RAATTY 6 cut(s) 169, 1041, 1861, 2627, 2984, 3303
AseI ATTAAT 1 cut(s) 2583
AsiGI ACCGGT 1 cut(s) 1004
Asp700I GAANNNNTTC 3 cut(s) 926, 2538, 3807
AspA2I CCTAGG 3 cut(s) 38, 1112, 3221
AspLEI GCGC 4 cut(s) 109, 252, 618, 1996
AspS9I GGNCC 6 cut(s) 296, 1667, 2085, 4064, 4166, 4575
AsuC2I CCSGG 2 cut(s) 2545, 2546
AsuNHI GCTAGC 1 cut(s) 1186
AvaI CYCGRG 3 cut(s) 323, 2544, 3897
AvaII GGWCC 2 cut(s) 1667, 2085
AvrII CCTAGG 3 cut(s) 38, 1112, 3221
BaeGI GKGCMC 4 cut(s) 321, 533, 1004, 1816
BalI TGGCCA 5 cut(s) 1461, 2213, 2309, 3828, 4209
BamHI GGATCC 4 cut(s) 1268, 1564, 1595, 4357
BanI GGYRCC 5 cut(s) 42, 249, 411, 417, 3565
BanII GRGCYC 3 cut(s) 237, 1229, 3886
BauI CACGAG 2 cut(s) 672, 2760
BbrPI CACGTG 1 cut(s) 2870
BbsI GAAGAC 1 cut(s) 2568
Bbv12I GWGCWC 5 cut(s) 321, 533, 1004, 1034, 1816
BceAI ACGGC 4 cut(s) 83, 399, 1714, 2217
BciVI GTATCC 3 cut(s) 1306, 2921, 4091
BcnI CCSGG 2 cut(s) 2545, 2546
BcoDI GTCTC 9 cut(s) 58, 145, 452, 1437, 2607, 3236, 4398, 4640, 4720
BcuI ACTAGT 1 cut(s) 3911
BfaI CTAG 9 cut(s) 39, 1113, 1187, 1236, 1586, 2043, 3222, 3912, 3959
BfmI CTRYAG 7 cut(s) 954, 1145, 1401, 1483, 1840, 2961, 4287
BfoI RGCGCY 2 cut(s) 110, 253
BfrI CTTAAG 2 cut(s) 649, 791
BfuAI ACCTGC 2 cut(s) 689, 3765
BfuI GTATCC 3 cut(s) 1306, 2921, 4091
BglI GCCNNNNNGGC 2 cut(s) 104, 2315
BglII AGATCT 2 cut(s) 1384, 1643
BlnI CCTAGG 3 cut(s) 38, 1112, 3221
Bme18I GGWCC 2 cut(s) 1667, 2085
BmeRI GACNNNNNGTC 1 cut(s) 567
BmeT110I CYCGRG 3 cut(s) 323, 2544, 3897
BmgBI CACGTC 4 cut(s) 52, 1490, 2815, 3535
BmgT120I GGNCC 6 cut(s) 296, 1667, 2085, 4064, 4166, 4575
BmrI ACTGGG 3 cut(s) 3010, 3649, 4417
BmtI GCTAGC 1 cut(s) 1190
BmuI ACTGGG 3 cut(s) 3010, 3649, 4417
BoxI GACNNNNGTC 1 cut(s) 1345
BpiI GAAGAC 1 cut(s) 2568
BplI GAGNNNNNCTC 2 cut(s) 3868, 3900
Bpu10I CCTNAGC 1 cut(s) 2166
BpuEI CTTGAG 2 cut(s) 820, 3767
BpuMI CCSGG 2 cut(s) 2545, 2546
BsaAI YACGTR 3 cut(s) 2426, 2870, 2918
BsaBI GATNNNNATC 2 cut(s) 1563, 3046
BsaHI GRCGYC 3 cut(s) 250, 506, 1877
BsaI GGTCTC 2 cut(s) 3236, 4720
BsaWI WCCGGW 4 cut(s) 84, 1004, 3148, 4710
Bse118I RCCGGY 4 cut(s) 298, 414, 904, 1004
Bse1I ACTGG 9 cut(s) 492, 1982, 2310, 3005, 3644, 3689, 3853, 4339, 4412
Bse3DI GCAATG 4 cut(s) 625, 666, 1880, 2516
Bse8I GATNNNNATC 2 cut(s) 1563, 3046
BseAI TCCGGA 1 cut(s) 84
BseJI GATNNNNATC 2 cut(s) 1563, 3046
BseMI GCAATG 4 cut(s) 625, 666, 1880, 2516
BseMII CTCAG 2 cut(s) 2180, 4307
BseNI ACTGG 9 cut(s) 492, 1982, 2310, 3005, 3644, 3689, 3853, 4339, 4412
BseRI GAGGAG 8 cut(s) 133, 470, 690, 2118, 3167, 3987, 4403, 4517
BseSI GKGCMC 4 cut(s) 321, 533, 1004, 1816
BseYI CCCAGC 3 cut(s) 365, 2175, 4292
BsgI GTGCAG 1 cut(s) 1518
Bsh1236I CGCG 4 cut(s) 247, 264, 275, 1222
BshNI GGYRCC 5 cut(s) 42, 249, 411, 417, 3565
BshTI ACCGGT 1 cut(s) 1004
BsiHKAI GWGCWC 5 cut(s) 321, 533, 1004, 1034, 1816
BsiHKCI CYCGRG 3 cut(s) 323, 2544, 3897
BsiSI CCGG 9 cut(s) 85, 285, 299, 415, 905, 1005, 2545, 3149, 4711
BslFI GGGAC 8 cut(s) 574, 1653, 2051, 2396, 2690, 3011, 4604, 4606
BsmAI GTCTC 9 cut(s) 58, 145, 452, 1437, 2607, 3236, 4398, 4640, 4720
BsmBI CGTCTC 4 cut(s) 58, 452, 1437, 4640
BsmFI GGGAC 8 cut(s) 574, 1653, 2051, 2396, 2690, 3011, 4604, 4606
Bso31I GGTCTC 2 cut(s) 3236, 4720
BsoBI CYCGRG 3 cut(s) 323, 2544, 3897
Bsp1286I GDGCHC 8 cut(s) 237, 321, 533, 1004, 1034, 1229, 1816, 3886
Bsp13I TCCGGA 1 cut(s) 84
Bsp1407I TGTACA 3 cut(s) 860, 983, 3727
Bsp19I CCATGG 3 cut(s) 1016, 3134, 4730
BspCNI CTCAG 2 cut(s) 2179, 4308
BspEI TCCGGA 1 cut(s) 84
BspFNI CGCG 4 cut(s) 247, 264, 275, 1222
BspHI TCATGA 2 cut(s) 2026, 2752
BspMAI CTGCAG 3 cut(s) 1149, 1405, 2965
BspMI ACCTGC 2 cut(s) 689, 3765
BspOI GCTAGC 1 cut(s) 1190
BspQI GCTCTTC 2 cut(s) 1476, 2544
BspT107I GGYRCC 5 cut(s) 42, 249, 411, 417, 3565
BspTI CTTAAG 2 cut(s) 649, 791
BspTNI GGTCTC 2 cut(s) 3236, 4720
BsrDI GCAATG 4 cut(s) 625, 666, 1880, 2516
BsrFI RCCGGY 4 cut(s) 298, 414, 904, 1004
BsrGI TGTACA 3 cut(s) 860, 983, 3727
BsrI ACTGG 9 cut(s) 492, 1982, 2310, 3005, 3644, 3689, 3853, 4339, 4412
BssAI RCCGGY 4 cut(s) 298, 414, 904, 1004
BssNAI GTATAC 2 cut(s) 1803, 3086
BssNI GRCGYC 3 cut(s) 250, 506, 1877
BssSI CACGAG 2 cut(s) 672, 2760
BssT1I CCWWGG 7 cut(s) 38, 1016, 1112, 2843, 3134, 3221, 4730
Bst1107I GTATAC 2 cut(s) 1803, 3086
Bst2BI CACGAG 2 cut(s) 672, 2760
Bst4CI ACNGT 8 cut(s) 422, 1421, 1855, 2507, 2887, 3692, 3725, 4629
Bst6I CTCTTC 5 cut(s) 1476, 1944, 2113, 2544, 4380
BstACI GRCGYC 3 cut(s) 250, 506, 1877
BstAFI CTTAAG 2 cut(s) 649, 791
BstAPI GCANNNNNTGC 2 cut(s) 2137, 2408
BstAUI TGTACA 3 cut(s) 860, 983, 3727
BstBAI YACGTR 3 cut(s) 2426, 2870, 2918
BstDEI CTNAG 5 cut(s) 869, 2166, 2456, 2767, 4316
BstDSI CCRYGG 6 cut(s) 262, 1016, 1220, 3134, 4625, 4730
BstENI CCTNNNNNAGG 1 cut(s) 2570
BstFNI CGCG 4 cut(s) 247, 264, 275, 1222
BstH2I RGCGCY 2 cut(s) 110, 253
BstHHI GCGC 4 cut(s) 109, 252, 618, 1996
BstMAI GTCTC 9 cut(s) 58, 145, 452, 1437, 2607, 3236, 4398, 4640, 4720
BstNSI RCATGY 1 cut(s) 4655
BstPAI GACNNNNGTC 1 cut(s) 1345
BstSFI CTRYAG 7 cut(s) 954, 1145, 1401, 1483, 1840, 2961, 4287
BstSLI GKGCMC 4 cut(s) 321, 533, 1004, 1816
BstUI CGCG 4 cut(s) 247, 264, 275, 1222
BstV2I GAAGAC 1 cut(s) 2568
BstXI CCANNNNNNTGG 4 cut(s) 897, 3135, 3257, 3324
BstZ17I GTATAC 2 cut(s) 1803, 3086
BsuI GTATCC 3 cut(s) 1306, 2921, 4091
BtgI CCRYGG 6 cut(s) 262, 1016, 1220, 3134, 4625, 4730
BtgZI GCGATG 2 cut(s) 219, 1272
BtrI CACGTC 4 cut(s) 52, 1490, 2815, 3535
BtsI GCAGTG 4 cut(s) 393, 450, 1059, 1989
BtsIMutI CAGTG 8 cut(s) 393, 450, 1059, 1426, 1989, 2303, 2512, 3730
BveI ACCTGC 2 cut(s) 689, 3765
CaiI CAGNNNCTG 1 cut(s) 2946
CciI TCATGA 2 cut(s) 2026, 2752
CfoI GCGC 4 cut(s) 109, 252, 618, 1996
Cfr10I RCCGGY 4 cut(s) 298, 414, 904, 1004
Cfr13I GGNCC 6 cut(s) 296, 1667, 2085, 4064, 4166, 4575
Cfr42I CCGCGG 2 cut(s) 265, 1223
Cfr9I CCCGGG 1 cut(s) 2544
CseI GACGC 5 cut(s) 264, 495, 1885, 1970, 4657
CspAI ACCGGT 1 cut(s) 1004
DdeI CTNAG 5 cut(s) 869, 2166, 2456, 2767, 4316
DinI GGCGCC 1 cut(s) 251
DraI TTTAAA 2 cut(s) 2016, 2632
DraIII CACNNNGTG 1 cut(s) 424
DriI GACNNNNNGTC 1 cut(s) 567
EaeI YGGCCR 6 cut(s) 1459, 1699, 2211, 2307, 3826, 4207
Eam1104I CTCTTC 5 cut(s) 1476, 1944, 2113, 2544, 4380
Eam1105I GACNNNNNGTC 1 cut(s) 567
EarI CTCTTC 5 cut(s) 1476, 1944, 2113, 2544, 4380
EciI GGCGGA 4 cut(s) 136, 358, 467, 1190
Eco130I CCWWGG 7 cut(s) 38, 1016, 1112, 2843, 3134, 3221, 4730
Eco24I GRGCYC 3 cut(s) 237, 1229, 3886
Eco31I GGTCTC 2 cut(s) 3236, 4720
Eco32I GATATC 1 cut(s) 3430
Eco47I GGWCC 2 cut(s) 1667, 2085
Eco57I CTGAAG 4 cut(s) 2580, 3160, 3381, 3484
Eco72I CACGTG 1 cut(s) 2870
Eco88I CYCGRG 3 cut(s) 323, 2544, 3897
EcoNI CCTNNNNNAGG 1 cut(s) 2570
EcoO109I RGGNCCY 2 cut(s) 2085, 4575
EcoRI GAATTC 1 cut(s) 2984
EcoRV GATATC 1 cut(s) 3430
EcoT14I CCWWGG 7 cut(s) 38, 1016, 1112, 2843, 3134, 3221, 4730
EcoT22I ATGCAT 2 cut(s) 970, 3232
EcoT38I GRGCYC 3 cut(s) 237, 1229, 3886
EgeI GGCGCC 1 cut(s) 251
EheI GGCGCC 1 cut(s) 251
ErhI CCWWGG 7 cut(s) 38, 1016, 1112, 2843, 3134, 3221, 4730
Esp3I CGTCTC 4 cut(s) 58, 452, 1437, 4640
FalI AAGNNNNNCTT 4 cut(s) 4359, 4391, 4377, 4409
FaqI GGGAC 8 cut(s) 574, 1653, 2051, 2396, 2690, 3011, 4604, 4606
FauI CCCGC 8 cut(s) 281, 389, 495, 1333, 1399, 1647, 2088, 4136
FauNDI CATATG 1 cut(s) 2656
FblI GTMKAC 3 cut(s) 1486, 1802, 3085
FriOI GRGCYC 3 cut(s) 237, 1229, 3886
FspBI CTAG 9 cut(s) 39, 1113, 1187, 1236, 1586, 2043, 3222, 3912, 3959
GlaI GCGC 4 cut(s) 108, 251, 617, 1995
GsaI CCCAGC 3 cut(s) 369, 2179, 4296
HaeII RGCGCY 2 cut(s) 110, 253
HapII CCGG 9 cut(s) 85, 285, 299, 415, 905, 1005, 2545, 3149, 4711
HgaI GACGC 5 cut(s) 264, 495, 1885, 1970, 4657
HhaI GCGC 4 cut(s) 109, 252, 618, 1996
Hin1I GRCGYC 3 cut(s) 250, 506, 1877
Hin6I GCGC 4 cut(s) 107, 250, 616, 1994
HinP1I GCGC 4 cut(s) 107, 250, 616, 1994
HindIII AAGCTT 3 cut(s) 1261, 3393, 4344
HpaII CCGG 9 cut(s) 85, 285, 299, 415, 905, 1005, 2545, 3149, 4711
Hpy99I CGWCG 4 cut(s) 62, 65, 233, 1964
HpyCH4III ACNGT 8 cut(s) 422, 1421, 1855, 2507, 2887, 3692, 3725, 4629
HpyF3I CTNAG 5 cut(s) 869, 2166, 2456, 2767, 4316
Hsp92I GRCGYC 3 cut(s) 250, 506, 1877
HspAI GCGC 4 cut(s) 107, 250, 616, 1994
KasI GGCGCC 1 cut(s) 249
Kpn2I TCCGGA 1 cut(s) 84
KroI GCCGGC 1 cut(s) 414
KroNI GCCGGC 1 cut(s) 416
KspI CCGCGG 2 cut(s) 265, 1223
LguI GCTCTTC 2 cut(s) 1476, 2544
MaeI CTAG 9 cut(s) 39, 1113, 1187, 1236, 1586, 2043, 3222, 3912, 3959
MfeI CAATTG 2 cut(s) 1965, 2610
MhlI GDGCHC 8 cut(s) 237, 321, 533, 1004, 1034, 1229, 1816, 3886
MlsI TGGCCA 5 cut(s) 1461, 2213, 2309, 3828, 4209
MluI ACGCGT 1 cut(s) 273
MluNI TGGCCA 5 cut(s) 1461, 2213, 2309, 3828, 4209
Mly113I GGCGCC 1 cut(s) 250
MlyI GAGTC 8 cut(s) 556, 624, 968, 2073, 3013, 3396, 3609, 4191
Mox20I TGGCCA 5 cut(s) 1461, 2213, 2309, 3828, 4209
Mph1103I ATGCAT 2 cut(s) 970, 3232
MroI TCCGGA 1 cut(s) 84
MroNI GCCGGC 1 cut(s) 414
MroXI GAANNNNTTC 3 cut(s) 926, 2538, 3807
MscI TGGCCA 5 cut(s) 1461, 2213, 2309, 3828, 4209
Msp20I TGGCCA 5 cut(s) 1461, 2213, 2309, 3828, 4209
MspA1I CMGCKG 4 cut(s) 264, 1222, 1406, 2348
MspCI CTTAAG 2 cut(s) 649, 791
MspI CCGG 9 cut(s) 85, 285, 299, 415, 905, 1005, 2545, 3149, 4711
MunI CAATTG 2 cut(s) 1965, 2610
MvnI CGCG 4 cut(s) 247, 264, 275, 1222
NaeI GCCGGC 1 cut(s) 416
NarI GGCGCC 1 cut(s) 250
NciI CCSGG 2 cut(s) 2545, 2546
NcoI CCATGG 3 cut(s) 1016, 3134, 4730
NdeI CATATG 1 cut(s) 2656
NgoMIV GCCGGC 1 cut(s) 414
NheI GCTAGC 1 cut(s) 1186
NmuCI GTSAC 8 cut(s) 827, 1412, 2522, 3052, 3185, 3526, 3635, 4753
NsiI ATGCAT 2 cut(s) 970, 3232
NspI RCATGY 1 cut(s) 4655
OliI CACNNNNGTG 3 cut(s) 1890, 3252, 3533
PaeI GCATGC 1 cut(s) 4655
PaeR7I CTCGAG 1 cut(s) 3897
PagI TCATGA 2 cut(s) 2026, 2752
PaqCI CACCTGC 2 cut(s) 689, 3765
PciSI GCTCTTC 2 cut(s) 1476, 2544
PcsI WCGNNNNNNNCGW 3 cut(s) 57, 69, 814
PdiI GCCGGC 1 cut(s) 416
PdmI GAANNNNTTC 3 cut(s) 926, 2538, 3807
PfeI GAWTC 7 cut(s) 112, 715, 922, 1769, 3893, 4137, 4462
PflMI CCANNNNNTGG 6 cut(s) 1017, 1022, 2849, 2946, 3463, 4736
PfoI TCCNGGA 2 cut(s) 2929, 3598
PinAI ACCGGT 1 cut(s) 1004
PleI GAGTC 8 cut(s) 556, 624, 967, 2072, 3013, 3395, 3609, 4191
PluTI GGCGCC 1 cut(s) 253
PmaCI CACGTG 1 cut(s) 2870
PmlI CACGTG 1 cut(s) 2870
PpsI GAGTC 8 cut(s) 556, 624, 967, 2072, 3013, 3395, 3609, 4191
Ppu21I YACGTR 3 cut(s) 2426, 2870, 2918
PpuMI RGGWCCY 1 cut(s) 2085
PshAI GACNNNNGTC 1 cut(s) 1345
PshBI ATTAAT 1 cut(s) 2583
PsiI TTATAA 3 cut(s) 2361, 2783, 3908
Psp5II RGGWCCY 1 cut(s) 2085
PspCI CACGTG 1 cut(s) 2870
PspFI CCCAGC 3 cut(s) 365, 2175, 4292
PspPI GGNCC 6 cut(s) 296, 1667, 2085, 4064, 4166, 4575
PspPPI RGGWCCY 1 cut(s) 2085
PstI CTGCAG 3 cut(s) 1149, 1405, 2965
PstNI CAGNNNCTG 1 cut(s) 2946
SacII CCGCGG 2 cut(s) 265, 1223
SapI GCTCTTC 2 cut(s) 1476, 2544
Sau96I GGNCC 6 cut(s) 296, 1667, 2085, 4064, 4166, 4575
SchI GAGTC 8 cut(s) 556, 624, 968, 2073, 3013, 3396, 3609, 4191
SduI GDGCHC 8 cut(s) 237, 321, 533, 1004, 1034, 1229, 1816, 3886
SfcI CTRYAG 7 cut(s) 954, 1145, 1401, 1483, 1840, 2961, 4287
SfoI GGCGCC 1 cut(s) 251
Sfr274I CTCGAG 1 cut(s) 3897
Sfr303I CCGCGG 2 cut(s) 265, 1223
SgrBI CCGCGG 2 cut(s) 265, 1223
SinI GGWCC 2 cut(s) 1667, 2085
SlaI CTCGAG 1 cut(s) 3897
SmaI CCCGGG 1 cut(s) 2546
SmlI CTYRAG 5 cut(s) 649, 791, 835, 3746, 3897
SmoI CTYRAG 5 cut(s) 649, 791, 835, 3746, 3897
SpeI ACTAGT 1 cut(s) 3911
SphI GCATGC 1 cut(s) 4655
SspDI GGCGCC 1 cut(s) 249
SspMI CTAG 9 cut(s) 39, 1113, 1187, 1236, 1586, 2043, 3222, 3912, 3959
StyI CCWWGG 7 cut(s) 38, 1016, 1112, 2843, 3134, 3221, 4730
TaaI ACNGT 8 cut(s) 422, 1421, 1855, 2507, 2887, 3692, 3725, 4629
TaqI TCGA 6 cut(s) 174, 817, 1066, 3670, 3898, 4469
TatI WGTACW 5 cut(s) 860, 983, 1149, 1774, 3727
TfiI GAWTC 7 cut(s) 112, 715, 922, 1769, 3893, 4137, 4462
TscAI CASTG 8 cut(s) 393, 450, 1059, 1426, 1996, 2310, 2512, 3730
TseFI GTSAC 8 cut(s) 827, 1412, 2522, 3052, 3185, 3526, 3635, 4753
Tsp45I GTSAC 8 cut(s) 827, 1412, 2522, 3052, 3185, 3526, 3635, 4753
TspGWI ACGGA 8 cut(s) 46, 61, 203, 2784, 3432, 3545, 3755, 4544
TspMI CCCGGG 1 cut(s) 2544
TspRI CASTG 8 cut(s) 393, 450, 1059, 1426, 1996, 2310, 2512, 3730
Van91I CCANNNNNTGG 6 cut(s) 1017, 1022, 2849, 2946, 3463, 4736
Vha464I CTTAAG 2 cut(s) 649, 791
VneI GTGCAC 4 cut(s) 317, 529, 1000, 1812
VpaK11BI GGWCC 2 cut(s) 1667, 2085
VspI ATTAAT 1 cut(s) 2583
XagI CCTNNNNNAGG 1 cut(s) 2570
XapI RAATTY 6 cut(s) 169, 1041, 1861, 2627, 2984, 3303
XceI RCATGY 1 cut(s) 4655
XcmI CCANNNNNNNNNTGG 8 cut(s) 736, 2850, 3254, 3681, 3870, 4277, 4446, 4632
XhoI CTCGAG 1 cut(s) 3897
XmaI CCCGGG 1 cut(s) 2544
XmaJI CCTAGG 3 cut(s) 38, 1112, 3221
XmiI GTMKAC 3 cut(s) 1486, 1802, 3085
XmnI GAANNNNTTC 3 cut(s) 926, 2538, 3807
XspI CTAG 9 cut(s) 39, 1113, 1187, 1236, 1586, 2043, 3222, 3912, 3959
Zsp2I ATGCAT 2 cut(s) 970, 3232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.