Rw1G005810

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0010072
Physical Location & Seq
Reverse (-)
11739567 .. 11752787
13221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G005810.1

Sequence Viewer

Length: 4908 bp
ATGAAGGTGCCGTGCTGTTCGGTGTGCCAGACGCGCTACGATGAGGAAGAGAGAGTGCCGCTGCTGCTGCAGTGCGGCCATGGCTTCTGCAAGGACTGCTTGTCCAAAATGTTCTCCGCCTGTCCCGACACCACGCTCGTCTGCCCTAGGTGCCGCCACGTCTCCGTCGTCGGCAACTCCGTCCAGGCCCTCCGGAAAAACTACGCCGTTCTGGCGCTGATTCACTCCAGCACCGCCTCCAATGGCGTCTCCTCCGCCGCCGCCGCCGCCGCAAATTTCGATTGCGATTACACGGACGACGAGGGCGGCGATGAGGATGAGGATGATGATAACGACGAGGCCTCGCTGTCGCGGCGCCGATGTAGCCGCGGATCAAACGCGTCCAGCTCCGGCGGGTGTGGGCCGGTCATTGAGGTGGCGGTGCACCCCGAGTTGAGGTTTGTGAGGCGGATAGGGGAGGGCAGGCAGGCTGGGGTTCAAATGTGGACGGCAGTGATCTGCGGGAGTGGGTGGCGGTGCCGGCACCGTGTGGCGGTGAAGAAGGTGGCGGTGGTGGCGGAGGAGACGAGTATGGATTGGGTGATGGGGCAGCTAGAGAACTTGCGGCGGGCGTCCATGTGGTGTAGGAATGTGTGCACATTTCATGGGGCGGTGAAGAATGAAGGGACTTTGTGTCTTGTTATGGATAAGTGTTATGGGTCTGTTCAATCCGAGATGGAGCGCAATGAGGGCAGACTCACTCTGGAGCAAATCTTAAGATATGGTGCTGACATTGCACGAGGAGTAGCTGAACTCCATGCCGCAAATGTTGTTTGTATGAATCTAAAACCATCCAATTTACTTTTGGATGCGAATGGTCATGCTGTGGTTTCTGATTATGGAGTTGCTGCTATTCTTAAGAAACCTTCCTGTCGCAAAACTCGATCAGAATGTGACACCTCAAGGATCCATTCGTGTATGGAGTGTACAATGCTAAGTCCTCACTATGCAGCACCAGAGGCATGGGAGCCGGTGAAGAAGTCACTGAATCCATTCTGGGATGAAGCTATTGGTATCTCTGCAGAGTCAGATGCATGGAGTTTTGGTTGTACATTGGTAGAAATGTGCACCGGTTCCATCCCATGGGCTGGTTTGAGCACAGAGGAAATTTACAAGGCGGTGGTCAAGGCTCGAAAACTGCCTCCACAATATGCAAGTGTAGTAGGTGTTGGAATACCTAGGGAATTGTGGAAAATGATTGGTGAGTGCCTGCAGTACAAGGCATCAAAAAGACCATCCTTTAATTTAATGCTAGCAACTTTCCTCCGCCATTTGCAGGAGATACCGCGTAGCTCTCCTGCTAGTCCTGATAATGACGTAACTAAAAGCTTGGGATCCAATGTGAAGGAACCATCGCCTGTATCCTATTCAAAAGTATTTCAGGGCAATCCCGCCCTTCTGCATAGACTTGTGTCCGAAGGGGATGTATATGGTGTTAGAGATCTGCTTGGAAAGGCTGCAGCGGGAAGTGACAACAGTGTAATATCGTCTCTATTAGAAGCTCAAAATGCTGATGGTCAAACTGCTCTTCATTTGGCCTGTAGACGTGGTAGTCCAGAACTTGTCGATGCTATTTTGGAGTATAGAGAGGCAAATGTAGATGTGTTGGACAAAGATGGGGATCCTCCACTTGTTTTTGCTCTAGTAGCTGGATCCCCAGAGTGTGTTCATGTTCTCATTAAAAGAGGTGCTAATGTGAGATCTCGGTTGCGGGAGGGCTTTGGTCCCTCTGTTGCTCATGTTTGTGCTTATCACGGCCAGCCTGATTGTATGCGTGAGTTACTAATGGCTGGAGCTGATCCTAATGCGGTGGATGAGGAAGGGGAATCTGTACTCCATAGAGCTATTACCAAGAAGTATACAGATTGTGCACTTGTGGTTTTGGAAAATGGGGGCTGTAGGTCAATGACTGTTCTAAATTCAGAAAAATTGACGCCATTGCACTTGTGTGTGCAAACATGGAATGTTGCTGTTGTTAGAAGATGGGTGGAAGTTGCAACACCAGAAGAGATTGCCGACGCAATTGATATACCAAGTCCAGTTGGCACTGCACTGTGTATGGCTGCTGCTCTAAAGAAAGATCATGAAATTGAAGGAAGAGAATTGGTGCGTATTTTGCTTGCTTCTGGAGCAGATCCAACTGCTCAGGATGCCCAGCATGGACGTACAGCTTTGCATACAGCTTCAATGGCCAATGACGTTGAGTTGGTGAAGATTATACTTGATGCGGGAGTTGATGTGAACATCCGAAATGCACAAAATACAATTCCACTTCATGTAGCACTGGCCAGAGGGGCGAAGTCATGTGTTGGGATGCTTCTATCCGCTGGAGCAAATTATAATCTACAGGATGATGAAGGGGACAATGCTTTCCATATAGCAGCAGATGCAGCAAAAATGATACGTGAAAATCTTGAATGGCTTATTGTCATGCTTAGAAATCCTGATGCTTCTGTTGAAGCCAGAAATCACAGTGGCAAGACATTGCGTGACTTTCTGGAAGCTCTTCCCCGTGAATGGGTATCTGAAGACCTGATGGAGGCACTTATTAATAGGGGAGTTTATCTGTCTCCTACAATATTTGAAGTGGGGGATTGGATCAAATTTAAAAGAAGTATCACAAATCCGACATATGGATGGCAAGGTGCAAAGCATAGAAGTGTAGGCTTCGTGCAAAGTGTCCCAGATAAGGACAATCTGATGGTATCATTTTGTTCTGGAGAGGCTCATGAGGCTCGTGTCTTAGCAAATGAAGTTATAAAAGTTATTCCATTAGACAGGGGGCAGCATGTCCAGCTTAAACCAGATGTTAAAGAGCCAAGGTTTGGGTGGCGTGGGCAATCACGTGATAGCATTGGTACTGTATTATGTGTTGATGATGATGGTATCCTACGTGTTGGGTTTCCTGGAGCCTCCAGAGGATGGAAAGCTGATCCTGCAGAAATGGAGAGAGTTGAAGAATTCAAAGTTGGGGACTGGGTTCGTATTCGCCCGACTCTCACAACTGCAAAGCATGGATTAGGATCTGTTACACCTGGGAGCATTGGTATTGTGTATTGTATACGACCAGATAGTAGTCTGTTGTTAGAATTAAGTTATCTTCCAAATCCATGGCATTGTGAACCAGAGGAGGTTGAGCCTGTAATCCCCTTCAGGATTGGTGACAGGGTATGTGTGAAGCGATCAGTTGCAGAACCTAGGTATGCATGGGGTGGTGAGACCCACCATAGTGTTGGAAGAATAAGTGAGATAGAGAATGATGGCCTCCTGGTTATTGAAATTCCAAACCGACCAATATCATGGCAGGCTGATCCTTCTGACATGGAGAAGTTGGAAGATTTCAAGGTAGGAGATTGGGTTAGAGTCAAAGCTTCAGTACCCTCCCCCAAATACGGATGGGAGGATATCACAAGGAGTAGTATTGGGATAATCCATAGCTTGGAGGAAGATGGTGATATGGGTGTTGCTTTTTGCTTCAGGAGCAAGCCTTTTTCTTGTTCTGTCACAGATGTGGAAAAGCTACCTCCTTTTGAGGTGCGACAAGAAATTCATATTCTGTCATCTGTCAGTCAGCCGCGACTTGGATGGTCAAATGAAAGTCCTGCAACTGTTGGAAAAATAGATAGAATTGACATGGATGGAGCTTTAAATGTAAGGGTGCCTGGCAGACAGAGCTTGTGGAAAGTTTCTCCAGGAGATGCTGAACGACTCTCTGGATTTGAAGTTGGTGACTGGGTACGATCAAAACCAAGCCTGGGAACGCGACCAAGTTATGACTGGAACAGTATTGGAAAAGAAAGTTTAGCTGTTGTTCACAGTGTACAAGACACAGGTTATCTTGAGTTGGCTTGCTGTTTCCGTAAAGGCAGGTGGATAACTCATTACACAGACGTTGAAAAGGTTCCATGCTTCAAAGTTGGCCAATATGTTCGGTTTCGTACTGGTTTAGTTGAACCAAGATGGGGATGGAGGGGGGCTCAACCTGATTCTCGAGGTATTATAACTAGTATTCATGCTGATGGGGAAGTGAGAGTGGCTTTCCCTGGTTTGCCAGGGTTGTGGAGAGGAGATCCTGCTGATTTTGAGATAGAGCAGATTTATGAAGTTGGAGAGTGGGTGAGATTAGAAGATCATGCAAATATGTGGAAATCTGTTGGGCCAGGGAGTGTTGGCGTGGTGCAAGGATTAGGATATGAGGGAGATAAATGGGATGGAACCACTTTTGTAGGATTCTGCGGGGAGCAAGAGAGATGGATTGGGCCTACTTCTGATCTTTCAAGGGCAAACAGGCTCTTGGTTGGCCAGAAGGTTAGGGTTAAATTATCTGTCAAGCAGCCCCGATTTGGATGGTCAGGCCACAGCCATGCAAGTGTTGGAACTATAGCCGGGATTGATGCTGATGGAAAGCTGAGAATATACACTCCAACTGGTTCAAAAGCTTGGATGTTGGATCCATCAGAAGTGCAACTTGTGGAGGAAGAGGAGCTTCATATTGGAGACTGGGTTAGGGTTAAAACATCAATATCAACCCCAACCCACCAATGGGGAGAGGTGAATCGTTCGAGCGTTGGGGTGGTGCATCGGATGGAAAATGAGGAGCTTTGGGTGGCTTTTTGCTTCACGGAGAGGTTGTGGCTTTGCAAGGCATTGGAAATGGAACGGGTCAGGCCCTTTAGAGTGGGGGACAAAGTGAGGATTAGAGAAGGGTTGGTGTCCCCACGGTGGGGTTGGGGAATGGAGACGCATGCCAGCAAAGGAGAGGTAGTTGGGGTGGATGCAAATGGAAAGTTAAGAATTAAGTTCCGGTGGAGAGAAGGGAGACCATGGATTGGAGATCCTGCTGATGTCACTCTTGATGAAAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Pkinase zf-C3HC4 zf-RING_2 zf-RING_5

Protein Analysis

1635

Amino Acids

180.4

Weight (kDa)

5.87

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 5 - 52 9.1e-06 Ring finger domain
zf-RING_UBOX PF13445 6 - 49 3.1e-06 RING-type zinc-finger
PK_Tyr_Ser-Thr PF07714 146 - 431 2.1e-29 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 172 - 431 1.2e-29 Protein kinase domain
Ank_2 PF12796 499 - 581 1.2e-12 Ankyrin repeats (3 copies)
Ank PF00023 518 - 551 4.5e-06 Ankyrin repeat
Ank_2 PF12796 593 - 673 2.4e-09 Ankyrin repeats (3 copies)
Ank_4 PF13637 593 - 637 5.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 712 - 797 7e-14 Ankyrin repeats (3 copies)
Ank PF00023 733 - 764 2e-06 Ankyrin repeat
Ank_4 PF13637 738 - 787 3.7e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 746 - 821 1.8e-07 Ankyrin repeats (3 copies)
SH3_15 PF18346 930 - 995 9.3e-14 Mind bomb SH3 repeat domain
SH3_15 PF18346 1064 - 1121 3.3e-08 Mind bomb SH3 repeat domain
SH3_15 PF18346 1127 - 1245 1.6e-11 Mind bomb SH3 repeat domain
SH3_15 PF18346 1253 - 1373 3.7e-16 Mind bomb SH3 repeat domain
SH3_15 PF18346 1380 - 1501 2.7e-16 Mind bomb SH3 repeat domain
SH3_15 PF18346 1510 - 1629 1.6e-14 Mind bomb SH3 repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2379, 2798, 4040
AarI CACCTGC 1 cut(s) 3897
AasI GACNNNNNNGTC 1 cut(s) 1590
Acc36I ACCTGC 1 cut(s) 3897
AccB1I GGYRCC 6 cut(s) 7, 150, 354, 516, 522, 3697
AccB7I CCANNNNNTGG 6 cut(s) 1122, 1127, 2864, 2961, 3478, 4868
AccI GTMKAC 3 cut(s) 1582, 1898, 3100
AccII CGCG 7 cut(s) 34, 352, 369, 380, 1327, 3616, 3802
AccIII TCCGGA 1 cut(s) 192
AcoI YGGCCR 6 cut(s) 76, 1795, 2229, 2325, 3958, 4339
AcsI RAATTY 7 cut(s) 274, 1146, 1957, 2642, 2999, 3318, 3585
AcuI CTGAAG 4 cut(s) 2586, 3175, 3396, 3499
AcvI CACGTG 1 cut(s) 2885
AcyI GRCGYC 4 cut(s) 246, 355, 611, 1973
AdeI CACNNNGTG 1 cut(s) 529
AflII CTTAAG 2 cut(s) 754, 896
AflIII ACRYGT 2 cut(s) 378, 2932
AgeI ACCGGT 1 cut(s) 1109
AhdI GACNNNNNGTC 2 cut(s) 100, 672
AhlI ACTAGT 1 cut(s) 4043
AjiI CACGTC 2 cut(s) 160, 1586
AjuI GAANNNNNNNTTGG 2 cut(s) 2991, 3023
AleI CACNNNNGTG 3 cut(s) 1986, 3267, 3548
Alw21I GWGCWC 5 cut(s) 426, 638, 1109, 1139, 1912
Alw26I GTCTC 9 cut(s) 166, 253, 557, 1533, 2613, 3251, 4530, 4772, 4852
Alw44I GTGCAC 4 cut(s) 422, 634, 1105, 1908
Ama87I CYCGRG 2 cut(s) 428, 4029
Aor13HI TCCGGA 1 cut(s) 192
ApaLI GTGCAC 4 cut(s) 422, 634, 1105, 1908
ApoI RAATTY 7 cut(s) 274, 1146, 1957, 2642, 2999, 3318, 3585
AseI ATTAAT 1 cut(s) 2589
AsiGI ACCGGT 1 cut(s) 1109
Asp700I GAANNNNTTC 3 cut(s) 1031, 2544, 3939
AspA2I CCTAGG 3 cut(s) 146, 1217, 3236
AspLEI GCGC 4 cut(s) 36, 217, 357, 723
AspS9I GGNCC 6 cut(s) 187, 401, 1763, 4196, 4298, 4707
AsuC2I CCSGG 1 cut(s) 4426
AsuNHI GCTAGC 1 cut(s) 1291
AvaI CYCGRG 2 cut(s) 428, 4029
AvaII GGWCC 1 cut(s) 1763
AvrII CCTAGG 3 cut(s) 146, 1217, 3236
BaeGI GKGCMC 4 cut(s) 426, 638, 1109, 1912
BalI TGGCCA 4 cut(s) 2231, 2327, 3960, 4341
BamHI GGATCC 5 cut(s) 945, 1373, 1660, 1691, 4489
BanI GGYRCC 6 cut(s) 7, 150, 354, 516, 522, 3697
BanII GRGCYC 1 cut(s) 4018
BauI CACGAG 2 cut(s) 777, 2775
BbrPI CACGTG 1 cut(s) 2885
BbsI GAAGAC 1 cut(s) 2574
Bbv12I GWGCWC 5 cut(s) 426, 638, 1109, 1139, 1912
BceAI ACGGC 3 cut(s) 191, 504, 1810
BciVI GTATCC 2 cut(s) 1411, 2936
BcnI CCSGG 1 cut(s) 4426
BcoDI GTCTC 9 cut(s) 166, 253, 557, 1533, 2613, 3251, 4530, 4772, 4852
BcuI ACTAGT 1 cut(s) 4043
BfaI CTAG 8 cut(s) 147, 593, 1218, 1292, 1341, 1682, 3237, 4044
BfmI CTRYAG 9 cut(s) 68, 1059, 1250, 1497, 1579, 1936, 2384, 2976, 4419
BfoI RGCGCY 2 cut(s) 218, 358
BfrI CTTAAG 2 cut(s) 754, 896
BfuAI ACCTGC 1 cut(s) 3897
BfuI GTATCC 2 cut(s) 1411, 2936
BglI GCCNNNNNGGC 2 cut(s) 212, 2333
BglII AGATCT 2 cut(s) 1480, 1739
BlnI CCTAGG 3 cut(s) 146, 1217, 3236
Bme18I GGWCC 1 cut(s) 1763
BmeRI GACNNNNNGTC 2 cut(s) 100, 672
BmeT110I CYCGRG 2 cut(s) 428, 4029
BmgBI CACGTC 2 cut(s) 160, 1586
BmgT120I GGNCC 6 cut(s) 187, 401, 1763, 4196, 4298, 4707
BmrI ACTGGG 3 cut(s) 3025, 3781, 4549
BmtI GCTAGC 1 cut(s) 1295
BmuI ACTGGG 3 cut(s) 3025, 3781, 4549
BoxI GACNNNNGTC 1 cut(s) 1450
BpiI GAAGAC 1 cut(s) 2574
BplI GAGNNNNNCTC 2 cut(s) 4000, 4032
BpmI CTGGAG 9 cut(s) 211, 764, 1851, 2187, 2388, 2778, 2938, 2967, 3714
Bpu10I CCTNAGC 1 cut(s) 2184
BpuEI CTTGAG 2 cut(s) 925, 3899
BpuMI CCSGG 1 cut(s) 4426
BsaAI YACGTR 3 cut(s) 2444, 2885, 2933
BsaBI GATNNNNATC 2 cut(s) 1659, 3061
BsaHI GRCGYC 4 cut(s) 246, 355, 611, 1973
BsaI GGTCTC 2 cut(s) 3251, 4852
BsaWI WCCGGW 3 cut(s) 192, 1109, 4842
Bse118I RCCGGY 4 cut(s) 403, 519, 1009, 1109
Bse1I ACTGG 8 cut(s) 2078, 2328, 3020, 3776, 3821, 3985, 4471, 4544
Bse3DI GCAATG 4 cut(s) 730, 771, 1976, 2522
Bse8I GATNNNNATC 2 cut(s) 1659, 3061
BseAI TCCGGA 1 cut(s) 192
BseJI GATNNNNATC 2 cut(s) 1659, 3061
BseMI GCAATG 4 cut(s) 730, 771, 1976, 2522
BseMII CTCAG 2 cut(s) 2198, 4439
BseNI ACTGG 8 cut(s) 2078, 2328, 3020, 3776, 3821, 3985, 4471, 4544
BseRI GAGGAG 7 cut(s) 241, 575, 795, 3182, 4119, 4535, 4649
BseSI GKGCMC 4 cut(s) 426, 638, 1109, 1912
BseYI CCCAGC 2 cut(s) 470, 2193
BsgI GTGCAG 1 cut(s) 2073
Bsh1236I CGCG 7 cut(s) 34, 352, 369, 380, 1327, 3616, 3802
BshNI GGYRCC 6 cut(s) 7, 150, 354, 516, 522, 3697
BshTI ACCGGT 1 cut(s) 1109
BsiHKAI GWGCWC 5 cut(s) 426, 638, 1109, 1139, 1912
BsiHKCI CYCGRG 2 cut(s) 428, 4029
BsiSI CCGG 8 cut(s) 193, 390, 404, 520, 1010, 1110, 4425, 4843
BslFI GGGAC 8 cut(s) 108, 679, 1749, 2414, 2705, 3026, 4736, 4738
BsmAI GTCTC 9 cut(s) 166, 253, 557, 1533, 2613, 3251, 4530, 4772, 4852
BsmBI CGTCTC 5 cut(s) 166, 253, 557, 1533, 4772
BsmFI GGGAC 8 cut(s) 108, 679, 1749, 2414, 2705, 3026, 4736, 4738
Bso31I GGTCTC 2 cut(s) 3251, 4852
BsoBI CYCGRG 2 cut(s) 428, 4029
Bsp1286I GDGCHC 6 cut(s) 426, 638, 1109, 1139, 1912, 4018
Bsp13I TCCGGA 1 cut(s) 192
Bsp1407I TGTACA 3 cut(s) 965, 1088, 3859
Bsp19I CCATGG 4 cut(s) 79, 1121, 3149, 4862
BspCNI CTCAG 2 cut(s) 2197, 4440
BspEI TCCGGA 1 cut(s) 192
BspFNI CGCG 7 cut(s) 34, 352, 369, 380, 1327, 3616, 3802
BspHI TCATGA 2 cut(s) 2122, 2767
BspMAI CTGCAG 5 cut(s) 72, 1063, 1254, 1501, 2980
BspMI ACCTGC 1 cut(s) 3897
BspOI GCTAGC 1 cut(s) 1295
BspQI GCTCTTC 2 cut(s) 1572, 2550
BspT107I GGYRCC 6 cut(s) 7, 150, 354, 516, 522, 3697
BspTI CTTAAG 2 cut(s) 754, 896
BspTNI GGTCTC 2 cut(s) 3251, 4852
BsrDI GCAATG 4 cut(s) 730, 771, 1976, 2522
BsrFI RCCGGY 4 cut(s) 403, 519, 1009, 1109
BsrGI TGTACA 3 cut(s) 965, 1088, 3859
BsrI ACTGG 8 cut(s) 2078, 2328, 3020, 3776, 3821, 3985, 4471, 4544
BssAI RCCGGY 4 cut(s) 403, 519, 1009, 1109
BssNAI GTATAC 2 cut(s) 1899, 3101
BssNI GRCGYC 4 cut(s) 246, 355, 611, 1973
BssSI CACGAG 2 cut(s) 777, 2775
BssT1I CCWWGG 8 cut(s) 79, 146, 1121, 1217, 2858, 3149, 3236, 4862
Bst1107I GTATAC 2 cut(s) 1899, 3101
Bst2BI CACGAG 2 cut(s) 777, 2775
Bst6I CTCTTC 6 cut(s) 42, 1572, 2040, 2131, 2550, 4512
BstACI GRCGYC 4 cut(s) 246, 355, 611, 1973
BstAFI CTTAAG 2 cut(s) 754, 896
BstAPI GCANNNNNTGC 2 cut(s) 96, 2426
BstAUI TGTACA 3 cut(s) 965, 1088, 3859
BstBAI YACGTR 3 cut(s) 2444, 2885, 2933
BstDEI CTNAG 5 cut(s) 974, 2184, 2474, 2782, 4448
BstDSI CCRYGG 6 cut(s) 79, 367, 1121, 3149, 4757, 4862
BstENI CCTNNNNNAGG 1 cut(s) 2576
BstFNI CGCG 7 cut(s) 34, 352, 369, 380, 1327, 3616, 3802
BstH2I RGCGCY 2 cut(s) 218, 358
BstHHI GCGC 4 cut(s) 36, 217, 357, 723
BstMAI GTCTC 9 cut(s) 166, 253, 557, 1533, 2613, 3251, 4530, 4772, 4852
BstNSI RCATGY 2 cut(s) 2831, 4787
BstPAI GACNNNNGTC 1 cut(s) 1450
BstSFI CTRYAG 9 cut(s) 68, 1059, 1250, 1497, 1579, 1936, 2384, 2976, 4419
BstSLI GKGCMC 4 cut(s) 426, 638, 1109, 1912
BstUI CGCG 7 cut(s) 34, 352, 369, 380, 1327, 3616, 3802
BstV2I GAAGAC 1 cut(s) 2574
BstXI CCANNNNNNTGG 5 cut(s) 1002, 3150, 3272, 3339, 4098
BstZ17I GTATAC 2 cut(s) 1899, 3101
BsuI GTATCC 2 cut(s) 1411, 2936
BtgI CCRYGG 6 cut(s) 79, 367, 1121, 3149, 4757, 4862
BtgZI GCGATG 2 cut(s) 324, 1377
BtrI CACGTC 2 cut(s) 160, 1586
BtsI GCAGTG 3 cut(s) 77, 498, 2085
BtsIMutI CAGTG 9 cut(s) 77, 498, 1022, 1522, 2085, 2090, 2321, 2518, 3862
BveI ACCTGC 1 cut(s) 3897
CciI TCATGA 2 cut(s) 2122, 2767
CfoI GCGC 4 cut(s) 36, 217, 357, 723
Cfr10I RCCGGY 4 cut(s) 403, 519, 1009, 1109
Cfr13I GGNCC 6 cut(s) 187, 401, 1763, 4196, 4298, 4707
Cfr42I CCGCGG 1 cut(s) 370
CseI GACGC 7 cut(s) 40, 235, 369, 600, 1981, 2066, 4789
CspAI ACCGGT 1 cut(s) 1109
DdeI CTNAG 5 cut(s) 974, 2184, 2474, 2782, 4448
DinI GGCGCC 1 cut(s) 356
DraI TTTAAA 2 cut(s) 2647, 3687
DraIII CACNNNGTG 1 cut(s) 529
DrdI GACNNNNNNGTC 1 cut(s) 1590
DriI GACNNNNNGTC 2 cut(s) 100, 672
DseDI GACNNNNNNGTC 1 cut(s) 1590
EaeI YGGCCR 6 cut(s) 76, 1795, 2229, 2325, 3958, 4339
Eam1104I CTCTTC 6 cut(s) 42, 1572, 2040, 2131, 2550, 4512
Eam1105I GACNNNNNGTC 2 cut(s) 100, 672
EarI CTCTTC 6 cut(s) 42, 1572, 2040, 2131, 2550, 4512
EciI GGCGGA 5 cut(s) 106, 244, 463, 572, 1295
Eco130I CCWWGG 8 cut(s) 79, 146, 1121, 1217, 2858, 3149, 3236, 4862
Eco147I AGGCCT 1 cut(s) 341
Eco24I GRGCYC 1 cut(s) 4018
Eco31I GGTCTC 2 cut(s) 3251, 4852
Eco32I GATATC 1 cut(s) 3445
Eco47I GGWCC 1 cut(s) 1763
Eco57I CTGAAG 4 cut(s) 2586, 3175, 3396, 3499
Eco72I CACGTG 1 cut(s) 2885
Eco88I CYCGRG 2 cut(s) 428, 4029
EcoNI CCTNNNNNAGG 1 cut(s) 2576
EcoO109I RGGNCCY 2 cut(s) 187, 4707
EcoRI GAATTC 1 cut(s) 2999
EcoRV GATATC 1 cut(s) 3445
EcoT14I CCWWGG 8 cut(s) 79, 146, 1121, 1217, 2858, 3149, 3236, 4862
EcoT22I ATGCAT 2 cut(s) 1075, 3247
EcoT38I GRGCYC 1 cut(s) 4018
EgeI GGCGCC 1 cut(s) 356
EheI GGCGCC 1 cut(s) 356
ErhI CCWWGG 8 cut(s) 79, 146, 1121, 1217, 2858, 3149, 3236, 4862
Esp3I CGTCTC 5 cut(s) 166, 253, 557, 1533, 4772
FalI AAGNNNNNCTT 6 cut(s) 83, 115, 4491, 4523, 4509, 4541
FaqI GGGAC 8 cut(s) 108, 679, 1749, 2414, 2705, 3026, 4736, 4738
FauI CCCGC 8 cut(s) 386, 494, 600, 1438, 1495, 1743, 2260, 4268
FauNDI CATATG 1 cut(s) 2671
FblI GTMKAC 3 cut(s) 1582, 1898, 3100
FriOI GRGCYC 1 cut(s) 4018
FspBI CTAG 8 cut(s) 147, 593, 1218, 1292, 1341, 1682, 3237, 4044
GlaI GCGC 4 cut(s) 35, 216, 356, 722
GsaI CCCAGC 2 cut(s) 474, 2197
GsuI CTGGAG 9 cut(s) 211, 764, 1851, 2187, 2388, 2778, 2938, 2967, 3714
HaeII RGCGCY 2 cut(s) 218, 358
HapII CCGG 8 cut(s) 193, 390, 404, 520, 1010, 1110, 4425, 4843
HgaI GACGC 7 cut(s) 40, 235, 369, 600, 1981, 2066, 4789
HhaI GCGC 4 cut(s) 36, 217, 357, 723
Hin1I GRCGYC 4 cut(s) 246, 355, 611, 1973
Hin6I GCGC 4 cut(s) 34, 215, 355, 721
HinP1I GCGC 4 cut(s) 34, 215, 355, 721
HindIII AAGCTT 3 cut(s) 1366, 3408, 4476
HpaII CCGG 8 cut(s) 193, 390, 404, 520, 1010, 1110, 4425, 4843
Hpy99I CGWCG 5 cut(s) 170, 173, 302, 338, 2060
HpyCH4IV ACGT 9 cut(s) 159, 1356, 1585, 2203, 2238, 2443, 2884, 2932, 3930
HpyF3I CTNAG 5 cut(s) 974, 2184, 2474, 2782, 4448
HpySE526I ACGT 9 cut(s) 159, 1356, 1585, 2203, 2238, 2443, 2884, 2932, 3930
Hsp92I GRCGYC 4 cut(s) 246, 355, 611, 1973
HspAI GCGC 4 cut(s) 34, 215, 355, 721
KasI GGCGCC 1 cut(s) 354
Kpn2I TCCGGA 1 cut(s) 192
KroI GCCGGC 1 cut(s) 519
KroNI GCCGGC 1 cut(s) 521
KspI CCGCGG 1 cut(s) 370
LguI GCTCTTC 2 cut(s) 1572, 2550
MaeI CTAG 8 cut(s) 147, 593, 1218, 1292, 1341, 1682, 3237, 4044
MaeII ACGT 9 cut(s) 159, 1356, 1585, 2203, 2238, 2443, 2884, 2932, 3930
MfeI CAATTG 1 cut(s) 2061
MhlI GDGCHC 6 cut(s) 426, 638, 1109, 1139, 1912, 4018
MlsI TGGCCA 4 cut(s) 2231, 2327, 3960, 4341
MluI ACGCGT 1 cut(s) 378
MluNI TGGCCA 4 cut(s) 2231, 2327, 3960, 4341
Mly113I GGCGCC 1 cut(s) 355
MlyI GAGTC 5 cut(s) 729, 1073, 3028, 3411, 3741
Mox20I TGGCCA 4 cut(s) 2231, 2327, 3960, 4341
Mph1103I ATGCAT 2 cut(s) 1075, 3247
MroI TCCGGA 1 cut(s) 192
MroNI GCCGGC 1 cut(s) 519
MroXI GAANNNNTTC 3 cut(s) 1031, 2544, 3939
MscI TGGCCA 4 cut(s) 2231, 2327, 3960, 4341
Msp20I TGGCCA 4 cut(s) 2231, 2327, 3960, 4341
MspA1I CMGCKG 4 cut(s) 61, 369, 1502, 2366
MspCI CTTAAG 2 cut(s) 754, 896
MspI CCGG 8 cut(s) 193, 390, 404, 520, 1010, 1110, 4425, 4843
MunI CAATTG 1 cut(s) 2061
MvnI CGCG 7 cut(s) 34, 352, 369, 380, 1327, 3616, 3802
NaeI GCCGGC 1 cut(s) 521
NarI GGCGCC 1 cut(s) 355
NciI CCSGG 1 cut(s) 4426
NcoI CCATGG 4 cut(s) 79, 1121, 3149, 4862
NdeI CATATG 1 cut(s) 2671
NgoMIV GCCGGC 1 cut(s) 519
NheI GCTAGC 1 cut(s) 1291
NmuCI GTSAC 8 cut(s) 932, 1020, 1508, 2528, 3200, 3541, 3767, 4885
NsiI ATGCAT 2 cut(s) 1075, 3247
NspI RCATGY 2 cut(s) 2831, 4787
OliI CACNNNNGTG 3 cut(s) 1986, 3267, 3548
PaeI GCATGC 1 cut(s) 4787
PaeR7I CTCGAG 1 cut(s) 4029
PagI TCATGA 2 cut(s) 2122, 2767
PaqCI CACCTGC 1 cut(s) 3897
PceI AGGCCT 1 cut(s) 341
PciSI GCTCTTC 2 cut(s) 1572, 2550
PcsI WCGNNNNNNNCGW 4 cut(s) 165, 177, 306, 919
PdiI GCCGGC 1 cut(s) 521
PdmI GAANNNNTTC 3 cut(s) 1031, 2544, 3939
PfeI GAWTC 7 cut(s) 220, 820, 1027, 1865, 4025, 4269, 4594
PflMI CCANNNNNTGG 6 cut(s) 1122, 1127, 2864, 2961, 3478, 4868
PfoI TCCNGGA 2 cut(s) 2944, 3730
PinAI ACCGGT 1 cut(s) 1109
PleI GAGTC 5 cut(s) 729, 1072, 3028, 3410, 3741
PluTI GGCGCC 1 cut(s) 358
PmaCI CACGTG 1 cut(s) 2885
PmlI CACGTG 1 cut(s) 2885
PpsI GAGTC 5 cut(s) 729, 1072, 3028, 3410, 3741
Ppu21I YACGTR 3 cut(s) 2444, 2885, 2933
PshAI GACNNNNGTC 1 cut(s) 1450
PshBI ATTAAT 1 cut(s) 2589
PsiI TTATAA 3 cut(s) 2379, 2798, 4040
PspCI CACGTG 1 cut(s) 2885
PspFI CCCAGC 2 cut(s) 470, 2193
PspPI GGNCC 6 cut(s) 187, 401, 1763, 4196, 4298, 4707
PstI CTGCAG 5 cut(s) 72, 1063, 1254, 1501, 2980
SacII CCGCGG 1 cut(s) 370
SapI GCTCTTC 2 cut(s) 1572, 2550
Sau96I GGNCC 6 cut(s) 187, 401, 1763, 4196, 4298, 4707
SchI GAGTC 5 cut(s) 729, 1073, 3028, 3411, 3741
SduI GDGCHC 6 cut(s) 426, 638, 1109, 1139, 1912, 4018
SfcI CTRYAG 9 cut(s) 68, 1059, 1250, 1497, 1579, 1936, 2384, 2976, 4419
SfoI GGCGCC 1 cut(s) 356
Sfr274I CTCGAG 1 cut(s) 4029
Sfr303I CCGCGG 1 cut(s) 370
SgrBI CCGCGG 1 cut(s) 370
SinI GGWCC 1 cut(s) 1763
SlaI CTCGAG 1 cut(s) 4029
SmlI CTYRAG 5 cut(s) 754, 896, 940, 3878, 4029
SmoI CTYRAG 5 cut(s) 754, 896, 940, 3878, 4029
SpeI ACTAGT 1 cut(s) 4043
SphI GCATGC 1 cut(s) 4787
SseBI AGGCCT 1 cut(s) 341
SspDI GGCGCC 1 cut(s) 354
SspI AATATT 1 cut(s) 2619
SspMI CTAG 8 cut(s) 147, 593, 1218, 1292, 1341, 1682, 3237, 4044
StuI AGGCCT 1 cut(s) 341
StyI CCWWGG 8 cut(s) 79, 146, 1121, 1217, 2858, 3149, 3236, 4862
TaiI ACGT 9 cut(s) 162, 1359, 1588, 2206, 2241, 2446, 2887, 2935, 3933
TaqI TCGA 6 cut(s) 279, 922, 1171, 1605, 4030, 4601
TatI WGTACW 5 cut(s) 965, 1088, 1254, 1870, 3859
TfiI GAWTC 7 cut(s) 220, 820, 1027, 1865, 4025, 4269, 4594
TscAI CASTG 9 cut(s) 77, 498, 1029, 1522, 2092, 2097, 2328, 2518, 3862
TseFI GTSAC 8 cut(s) 932, 1020, 1508, 2528, 3200, 3541, 3767, 4885
Tsp45I GTSAC 8 cut(s) 932, 1020, 1508, 2528, 3200, 3541, 3767, 4885
TspGWI ACGGA 6 cut(s) 154, 169, 308, 3447, 3887, 4676
TspRI CASTG 9 cut(s) 77, 498, 1029, 1522, 2092, 2097, 2328, 2518, 3862
Van91I CCANNNNNTGG 6 cut(s) 1122, 1127, 2864, 2961, 3478, 4868
Vha464I CTTAAG 2 cut(s) 754, 896
VneI GTGCAC 4 cut(s) 422, 634, 1105, 1908
VpaK11BI GGWCC 1 cut(s) 1763
VspI ATTAAT 1 cut(s) 2589
XagI CCTNNNNNAGG 1 cut(s) 2576
XapI RAATTY 7 cut(s) 274, 1146, 1957, 2642, 2999, 3318, 3585
XceI RCATGY 2 cut(s) 2831, 4787
XcmI CCANNNNNNNNNTGG 8 cut(s) 841, 2865, 3269, 3813, 4002, 4409, 4578, 4764
XhoI CTCGAG 1 cut(s) 4029
XmaJI CCTAGG 3 cut(s) 146, 1217, 3236
XmiI GTMKAC 3 cut(s) 1582, 1898, 3100
XmnI GAANNNNTTC 3 cut(s) 1031, 2544, 3939
XspI CTAG 8 cut(s) 147, 593, 1218, 1292, 1341, 1682, 3237, 4044
Zsp2I ATGCAT 2 cut(s) 1075, 3247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.