RLG00000030103

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0010072
Physical Location & Seq
Forward (+)
55352938 .. 55365613
12676 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000030103

Sequence Viewer

Length: 4908 bp
ATGAAGGTGCCGTGCTGTTCGGTGTGCCAGACGCGCTACGATGAGGAAGAGAGAGTGCCGCTGCTGCTGCAGTGCGGCCATGGCTTCTGCAAGGACTGCTTGTCCAAAATGTTCTCCGCCTGTCCCGACACCACGCTCGTCTGCCCTAGGTGCCGCCACGTCTCCGTCGTCGGCAACTCCGTCCAGGCCCTCCGGAAAAACTACGCCGTTCTGGCGCTGATTCACTCCAGCACCGCCTCCAATGGCGTCTCCTCCGCCGCCGCCGCCGCCGCAAATTTCGATTGCGATTATACGGACGACGAGGGCGGCGATGAGGATGAGGATGATGATAACGACGAGGCCTCGCTGTCGCGGCGCCGATGTAGCCGCGGATCAAACGCGTCCAGCTCCGGCGGGTGTGGGCCGGTCATTGAGGTGGCGGTGCACCCCGAGATGAGGCTTGTGAGGCGGATAGGGGAGGGGAGGCAGGCTGGGGTTCAAATGTGGACGGCGGTGATCGGCGGGAGTGGGTGGCGGTGCCGGCACCGTGTGGCGGTGAAGAAGGTGGCGGTGGTGGCGGAGGAGACGAGTATGGATTGGGTGATTGGGCAACTGGATAACTTGCGGCGGGCGTCCATGTGGTGTAGGAATGTGTGCACATTTCATGGGGCGGTGAAGAATGAAGGGACTTTGTGTCTTGTTATGGATAAGTGTTATGGGTCTGTTCAATCCGAGATGGAGCGCAATGAGGGAAGACTCACTCTGGAGCAAATCTTAAGATATGGTGCTGACATTGCACGAGGTGTAGCTGAACTCCATGCTGCAGGTGTTGTTTGTATGAATCTAAAACCATCCAATTTGCTTTTGGATGCGAATGGTCATGCTGTGGTTTCTGATTATGGAGTTGCTGCTATTCTTAAGAAACCTTCCTGTCGCAAAACTCGATCAGAATGTGACACCTCAAGGATCCATTCGTGTATGGAGTGTACAATGCTAAGTCCTCACTATGCAGCACCAGAGGCATGGGAGCCGGTGAAGAAGTTACTGAATCCATTCTGGGATGAAGCTATTGGTATCTCTGCAGAGTCAGATGCATGGAGTTTTGGTTGTACATTGGTAGAAATGTGCACCGGTTCCATCCCATGGGCTGGTTTGAGCACGGAGGAAATTTACAAGGCGGTGTTCAAGGCTCGAAAACTGCCTCCACAATATGCAAGTGTAGTAGGTGTTGGAATACCTAGGGAATTGTGGAAAATGATTGGTGAGTGCCTGCAGTACAAGGCATCAAAAAGACCATCCTTTAATTTAATGCTAGCAACTTTCCTCCGCCATTTGCAGGAGATACCGCGGAGCCCTCCTGCTAGTCCTGATAATGACGTAGCTAAAAGCTTGGGATCCAATGTGAAGGAACCATCGCCTGTATCCTATTCAAAAGTATTTCAGGGTAATCCCGCCCTTCTGCATAGACTTGTGTCCGAAGGGGATGTATATGGTGTTAGAGATCTGCTTGGAAAGGCTGCAGCGGGAAGTGACAACAGTGTAATATCGTCTCTATTAGAAGCTCAAAATGCTGATGGCCAAACTGCTCTTCATTTGGCCTGTAGACGTGGTAGTGCAGAACTTGTTGATGCTATTTTGGAGTATAGAGAGGCAAATGTAGATGTGTTGGACAAAGATGGGGATCCTCCACTTGTTTTTGCTCTAGTAGCTGGATCCCCAGAGTGTGTTCATGTTCTCATTAAAAGAGGTGCTAATGTGAGATCTCGGTTGCGGGAGGGCTTTGGTCCCTCTGTTGCTCATGTCTGTGCTTATCACGGCCAGCCTGATTGTATGCGTGAGTTACTAATGGCTGGAGCTGATCCTAATGCGGTGGATGAGGAAGGGGAATCGGTACTCCATAGAGCTATTACCAAGAAGTATACAGATTGTGCACTTGTGGTTTTGGAAAATGGGGGCTGTAGGTCAATGACTGTTCTAAATTCAGAAAAAATGACGCCATTGCACTTGTGTGTGCAAACATGGAATGTTGCTGTTGTTAGAAGATGGGTGGAAGTTGCAACACCAGAAGAGATTGCCGACGCAATTGATATACCAAGTCCAGTTGGCACTGTGCTGTGTATGGCTGCTGCTTTAAAGAAAGATCATGAAATTGAAGGAAGAGAATTGGTGCGTATTTTGCTTGCTTCTGGAGCAGATCCAACTGCTCAGGATGCCCAGCATGGACGTACAGCTTTGCATACGGCTTCTATGGCCAATGACGTTGAGTTGGTGAAGATTATACTTGATGCGGGAGTTGATGTGAACATCCGAAATGCACAAAATACAATTCCACTTCATGTAGCACTGGCCAGAGGGGCGAAGTCATGTGTTGGGATGCTTCTATCCGCTGGAGCAAATTATAATTTGCAGGATGATGAAGGGGACAATGCTTTCCATATAGCAGCAGATGCAGCAAAAATGATACGTGAAAATCTTGAATGGCTTATTGTCATGCTTAGAAATCCTGATGCTTCTGTTGAAGCCAGAAATCACAGTGGCAAGACATTGCGTGACTTTCTGGAAGCTCTTCCCCGGGAATGGGTATCTGAAGACCTGATGGAGGCACTTATTAATAGGGGAGTTTATCTGTCTCCTACAATATTTGAAGTGGGGGATTGGATCAAATTTAAAAGAAGTATCACAAATCCGACATATGGATGGCAAGGTGCAAAGCATAGAAGTGTAGGCTTCGTGCAAAGTGTCCCAGATAAGGACAATCTGATAGTATCATTTTGTTCTGGAGAGGCTCATGAGGCTCGTGTCTTGGCAAATGAAGTTATAAAGGTTATTCCGTTAGACAGGGGGCAGCATGTCCAGCTTAAACCCGATGTTAAAGAGCCAAGGTTTGGGTGGCGTGGGCAATCACGTGATAGCATTGGTACTGTATTATGTGTTGATGATGATGGTATCCTACGTGTTGGGTTTCCGGGAGCCTCCAGAGGATGGAAAGCTGATCCTGCAGAAATGGAGAGAGTTGAAGAATTCAAGGTTGGGGACTGGGTTCGTATTCGCCCAACTCTTACAACAGCAAAGCATGGATTAGGATCTGTCACACCTGGGAGCATTGGTATTGTGTATTGTATACGACCAGATAGTAGTCTGTTGTTAGAATTAAGTTATCTTCCAAATCCATGGCATTGTGAACCGGAGGAGGTTGAGCCTGTAATCCCCTTCAGGATTGGTGACAGGGTATGTGTGAAGCGATCAGTTGCAGAACCTAGGTATGCATGGGGTGGTGAGACCCACCATAGTGTTGGAAGAATAAGTGAGATAGAGAATGATGGCCTCCTGGTTATTGAAATTCCAAACCGACCAATATCATGGCAGGCTGATCCTTCTGACATGGAGAAGTTGGAAGATTTCAAGGTAGGAGATTGGGTTAGGGTCAAAGCTTCAGTACCCTCCCCCAAATACGGATGGGAGGATATCACGAGGAGTAGTATTGGGATAATCCATAGCTTGGAGGAAGATGGTGATATGGGTGTGGCTTTTTGCTTCAGGAGCAAGCCTTTTTCTTGTTCTGTCACAGATGTGGAAAAGCTACCTCCTTTTGAGGTGGGACAAGAAATTCATATTTTGTCATCTATCAGTCAGCCACGACTTGGATGGTCAAATGAAAGTCCTGCGACTGTTGGAAAAATAGATAGAATTGACATGGATGGAGCTTTAAATGTAAGGGTGCCTGGCAGACAGAGCTTGTGGAAAGTTTCTCCAGGAGATGCTGAACGACTCTCTGGATTTGAAGTTGGTGACTGGGTACGATCAAAACCAAGCCTGGGAACTCGACCAAGTTATGACTGGAACAGTATTGGAAAAGAAAGCTTAGCTGTTGTTCACAGTGTACAAGACACAGGTTATCTTGAGTTGGCTTGCTGTTTCCGTAAAGGCAGGTGGATAACTCATTATACAGACGTTGAAAAGGTTCCATGCTTCAAAGTTGGCCAATATGTTCGGTTTCGTACTGGTTTAGTTGAACCAAGATGGGGATGGAGAGGGGCTCAACCTGATTCTCGAGGTATTATAACTAGTATTCATGCTGATGGGGAAGTGAGAGTGGCTTTCCCTGGTTTGCCAGGGTTGTGGAGAGGAGATCCTGCTGATTTTGAGATAGAGCAGATTTATGACGTTGGAGAGTGGGTGAGATTAGAAGATCATGCAAATATGTGGAAATCTGTTGGGCCAGGGAGTGTTGGCGTGGTGCAAGGATTAGGATATGAGGGAGATAAATGGGATGGAACCACTTTTGTAGGATTCTGCGGGGAGCAAGAGAGATGGATTGGGCCTACTTCTGATCTTGCAAGGGCAAACAGGCTCTTGGTTGGCCAGAAGGTTAGGGTTAAATTATCTGTCAAGCAGCCTCGATTTGGATGGTCAGGCCACAGCCATGCAAGTGTTGGAACTATAGCCGGGATTGATGCTGATGGAAAGCTGAGAATATACACTCCAACTGGTTCAAAAGCTTGGATGTTGGATCCATCAGAAGTGCAACTTGTGGAGGAAGAGGAGCTTCATATTGGAGACTGGGTTAGGGTTAAAACATCAATATCAACCCCAACCCACCAATGGGGAGAGGTGAATCGTTCGAGCGTTGGGGTGGTGCATCGGATGGAAAATGAGGAGCTTTGGGTGGCTTTTTGCTTCACGGAGAGGTTGTGGCTTTGCAAGGCATCGGAAATGGAACGGGTCAGGCCCTTTAGAGTGGGGGACAAAGTGAGGATTAGAGAAGGGTTGGTGTCCCCACGGTGGGGTTGGGGAATGGAGACGCATGCCAGCAAAGGAGAGGTGGTTGGGGTGGATGCAAATGGAAAGTTGAGAATTAAGTTCCGGTGGAGAGAAGGGAGACCATGGATTGGAGATCCTGCTGATGTCACTCTTGATGAAAACACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Pkinase zf-C3HC4 zf-RING_2 zf-RING_5

Protein Analysis

1636

Amino Acids

180.16

Weight (kDa)

5.84

Isoelectric Point (pI)

42.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 5 - 52 9.1e-06 Ring finger domain
zf-RING_UBOX PF13445 6 - 49 3.1e-06 RING-type zinc-finger
PK_Tyr_Ser-Thr PF07714 147 - 431 3.1e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 172 - 431 2.6e-29 Protein kinase domain
Ank_2 PF12796 499 - 581 8.9e-13 Ankyrin repeats (3 copies)
Ank PF00023 518 - 551 6.5e-06 Ankyrin repeat
Ank_4 PF13637 593 - 637 5.6e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 593 - 673 9.4e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 712 - 797 6.1e-14 Ankyrin repeats (3 copies)
Ank PF00023 733 - 764 2e-06 Ankyrin repeat
Ank_4 PF13637 738 - 787 3.7e-07 Ankyrin repeats (many copies)
SH3_15 PF18346 930 - 995 9.3e-14 Mind bomb SH3 repeat domain
SH3_15 PF18346 1064 - 1121 3.3e-08 Mind bomb SH3 repeat domain
SH3_15 PF18346 1127 - 1245 4.8e-13 Mind bomb SH3 repeat domain
SH3_15 PF18346 1253 - 1373 3.7e-16 Mind bomb SH3 repeat domain
SH3_15 PF18346 1380 - 1501 7.8e-16 Mind bomb SH3 repeat domain
SH3_15 PF18346 1510 - 1629 1.9e-14 Mind bomb SH3 repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 2379, 2798, 4040
AarI CACCTGC 2 cut(s) 794, 3897
Acc36I ACCTGC 2 cut(s) 794, 3897
AccB1I GGYRCC 6 cut(s) 7, 150, 354, 516, 522, 3697
AccB7I CCANNNNNTGG 7 cut(s) 1122, 1127, 2864, 2961, 3478, 3620, 4868
AccI GTMKAC 3 cut(s) 1582, 1898, 3100
AccII CGCG 5 cut(s) 34, 352, 369, 380, 1327
AccIII TCCGGA 1 cut(s) 192
AcoI YGGCCR 7 cut(s) 76, 1555, 1795, 2229, 2325, 3958, 4339
AcsI RAATTY 7 cut(s) 274, 1146, 1957, 2642, 2999, 3318, 3585
AcuI CTGAAG 4 cut(s) 2586, 3175, 3396, 3499
AcvI CACGTG 1 cut(s) 2885
AcyI GRCGYC 4 cut(s) 246, 355, 611, 1973
AdeI CACNNNGTG 2 cut(s) 529, 782
AflII CTTAAG 2 cut(s) 754, 896
AflIII ACRYGT 2 cut(s) 378, 2932
AgeI ACCGGT 1 cut(s) 1109
AhdI GACNNNNNGTC 2 cut(s) 100, 672
AhlI ACTAGT 1 cut(s) 4043
AjiI CACGTC 2 cut(s) 160, 1586
AjnI CCWGG 9 cut(s) 183, 3073, 3306, 3700, 3730, 3792, 4081, 4090, 4198
AjuI GAANNNNNNNTTGG 4 cut(s) 1551, 1583, 2991, 3023
AleI CACNNNNGTG 3 cut(s) 1986, 3267, 3548
Alw21I GWGCWC 5 cut(s) 426, 638, 1109, 1139, 1912
Alw26I GTCTC 9 cut(s) 166, 253, 557, 1533, 2613, 3251, 4530, 4772, 4852
Alw44I GTGCAC 4 cut(s) 422, 634, 1105, 1908
Ama87I CYCGRG 3 cut(s) 428, 2550, 4029
Aor13HI TCCGGA 1 cut(s) 192
ApaLI GTGCAC 4 cut(s) 422, 634, 1105, 1908
ApoI RAATTY 7 cut(s) 274, 1146, 1957, 2642, 2999, 3318, 3585
AseI ATTAAT 1 cut(s) 2589
AsiGI ACCGGT 1 cut(s) 1109
Asp700I GAANNNNTTC 3 cut(s) 1031, 2544, 3939
AspA2I CCTAGG 3 cut(s) 146, 1217, 3236
AspLEI GCGC 4 cut(s) 36, 217, 357, 723
AspS9I GGNCC 6 cut(s) 187, 401, 1763, 4196, 4298, 4707
AsuC2I CCSGG 4 cut(s) 2551, 2552, 2946, 4426
AsuNHI GCTAGC 1 cut(s) 1291
AvaI CYCGRG 3 cut(s) 428, 2550, 4029
AvaII GGWCC 1 cut(s) 1763
AvrII CCTAGG 3 cut(s) 146, 1217, 3236
BaeGI GKGCMC 4 cut(s) 426, 638, 1109, 1912
BalI TGGCCA 5 cut(s) 1557, 2231, 2327, 3960, 4341
BamHI GGATCC 5 cut(s) 945, 1373, 1660, 1691, 4489
BanI GGYRCC 6 cut(s) 7, 150, 354, 516, 522, 3697
BanII GRGCYC 2 cut(s) 1334, 4018
BauI CACGAG 3 cut(s) 777, 2775, 3448
BbrPI CACGTG 1 cut(s) 2885
BbsI GAAGAC 2 cut(s) 739, 2574
Bbv12I GWGCWC 5 cut(s) 426, 638, 1109, 1139, 1912
BceAI ACGGC 4 cut(s) 191, 504, 1810, 2235
BciT130I CCWGG 9 cut(s) 185, 3075, 3308, 3702, 3732, 3794, 4083, 4092, 4200
BciVI GTATCC 2 cut(s) 1411, 2936
BcnI CCSGG 4 cut(s) 2551, 2552, 2946, 4426
BcoDI GTCTC 9 cut(s) 166, 253, 557, 1533, 2613, 3251, 4530, 4772, 4852
BcuI ACTAGT 1 cut(s) 4043
BfaI CTAG 7 cut(s) 147, 1218, 1292, 1341, 1682, 3237, 4044
BfmI CTRYAG 9 cut(s) 68, 801, 1059, 1250, 1497, 1579, 1936, 2976, 4419
BfoI RGCGCY 2 cut(s) 218, 358
BfrI CTTAAG 2 cut(s) 754, 896
BfuAI ACCTGC 2 cut(s) 794, 3897
BfuI GTATCC 2 cut(s) 1411, 2936
BglI GCCNNNNNGGC 2 cut(s) 212, 2333
BglII AGATCT 2 cut(s) 1480, 1739
BlnI CCTAGG 3 cut(s) 146, 1217, 3236
BlpI GCTNAGC 1 cut(s) 3841
Bme18I GGWCC 1 cut(s) 1763
BmeRI GACNNNNNGTC 2 cut(s) 100, 672
BmeT110I CYCGRG 3 cut(s) 428, 2550, 4029
BmgBI CACGTC 2 cut(s) 160, 1586
BmgT120I GGNCC 6 cut(s) 187, 401, 1763, 4196, 4298, 4707
BmrI ACTGGG 3 cut(s) 3025, 3781, 4549
BmtI GCTAGC 1 cut(s) 1295
BmuI ACTGGG 3 cut(s) 3025, 3781, 4549
BoxI GACNNNNGTC 1 cut(s) 1450
BpiI GAAGAC 2 cut(s) 739, 2574
BplI GAGNNNNNCTC 2 cut(s) 4000, 4032
BpmI CTGGAG 8 cut(s) 211, 764, 1851, 2187, 2388, 2778, 2938, 3714
Bpu10I CCTNAGC 1 cut(s) 2184
Bpu1102I GCTNAGC 1 cut(s) 3841
BpuEI CTTGAG 2 cut(s) 925, 3899
BpuMI CCSGG 4 cut(s) 2551, 2552, 2946, 4426
BsaAI YACGTR 3 cut(s) 2444, 2885, 2933
BsaBI GATNNNNATC 2 cut(s) 1659, 3061
BsaHI GRCGYC 4 cut(s) 246, 355, 611, 1973
BsaI GGTCTC 2 cut(s) 3251, 4852
BsaWI WCCGGW 4 cut(s) 192, 1109, 3163, 4842
Bse118I RCCGGY 4 cut(s) 403, 519, 1009, 1109
Bse1I ACTGG 9 cut(s) 597, 2078, 2328, 3020, 3776, 3821, 3985, 4471, 4544
Bse3DI GCAATG 4 cut(s) 730, 771, 1976, 2522
Bse8I GATNNNNATC 2 cut(s) 1659, 3061
BseAI TCCGGA 1 cut(s) 192
BseBI CCWGG 9 cut(s) 185, 3075, 3308, 3702, 3732, 3794, 4083, 4092, 4200
BseJI GATNNNNATC 2 cut(s) 1659, 3061
BseMI GCAATG 4 cut(s) 730, 771, 1976, 2522
BseMII CTCAG 2 cut(s) 2198, 4439
BseNI ACTGG 9 cut(s) 597, 2078, 2328, 3020, 3776, 3821, 3985, 4471, 4544
BseRI GAGGAG 7 cut(s) 241, 575, 3182, 3466, 4119, 4535, 4649
BseSI GKGCMC 4 cut(s) 426, 638, 1109, 1912
BseYI CCCAGC 2 cut(s) 470, 2193
BsgI GTGCAG 1 cut(s) 1614
Bsh1236I CGCG 5 cut(s) 34, 352, 369, 380, 1327
BshNI GGYRCC 6 cut(s) 7, 150, 354, 516, 522, 3697
BshTI ACCGGT 1 cut(s) 1109
BsiHKAI GWGCWC 5 cut(s) 426, 638, 1109, 1139, 1912
BsiHKCI CYCGRG 3 cut(s) 428, 2550, 4029
BslFI GGGAC 9 cut(s) 108, 679, 1749, 2414, 2705, 3026, 3591, 4736, 4738
BsmAI GTCTC 9 cut(s) 166, 253, 557, 1533, 2613, 3251, 4530, 4772, 4852
BsmBI CGTCTC 5 cut(s) 166, 253, 557, 1533, 4772
BsmFI GGGAC 9 cut(s) 108, 679, 1749, 2414, 2705, 3026, 3591, 4736, 4738
Bso31I GGTCTC 2 cut(s) 3251, 4852
BsoBI CYCGRG 3 cut(s) 428, 2550, 4029
Bsp1286I GDGCHC 7 cut(s) 426, 638, 1109, 1139, 1334, 1912, 4018
Bsp13I TCCGGA 1 cut(s) 192
Bsp1407I TGTACA 3 cut(s) 965, 1088, 3859
Bsp1720I GCTNAGC 1 cut(s) 3841
Bsp19I CCATGG 4 cut(s) 79, 1121, 3149, 4862
BspCNI CTCAG 2 cut(s) 2197, 4440
BspEI TCCGGA 1 cut(s) 192
BspFNI CGCG 5 cut(s) 34, 352, 369, 380, 1327
BspHI TCATGA 2 cut(s) 2122, 2767
BspMAI CTGCAG 6 cut(s) 72, 805, 1063, 1254, 1501, 2980
BspMI ACCTGC 2 cut(s) 794, 3897
BspOI GCTAGC 1 cut(s) 1295
BspQI GCTCTTC 2 cut(s) 1572, 2550
BspT107I GGYRCC 6 cut(s) 7, 150, 354, 516, 522, 3697
BspTI CTTAAG 2 cut(s) 754, 896
BspTNI GGTCTC 2 cut(s) 3251, 4852
BsrDI GCAATG 4 cut(s) 730, 771, 1976, 2522
BsrFI RCCGGY 4 cut(s) 403, 519, 1009, 1109
BsrGI TGTACA 3 cut(s) 965, 1088, 3859
BsrI ACTGG 9 cut(s) 597, 2078, 2328, 3020, 3776, 3821, 3985, 4471, 4544
BssAI RCCGGY 4 cut(s) 403, 519, 1009, 1109
BssNAI GTATAC 2 cut(s) 1899, 3101
BssNI GRCGYC 4 cut(s) 246, 355, 611, 1973
BssSI CACGAG 3 cut(s) 777, 2775, 3448
BssT1I CCWWGG 8 cut(s) 79, 146, 1121, 1217, 2858, 3149, 3236, 4862
Bst1107I GTATAC 2 cut(s) 1899, 3101
Bst2BI CACGAG 3 cut(s) 777, 2775, 3448
Bst2UI CCWGG 9 cut(s) 185, 3075, 3308, 3702, 3732, 3794, 4083, 4092, 4200
Bst6I CTCTTC 6 cut(s) 42, 1572, 2040, 2131, 2550, 4512
BstACI GRCGYC 4 cut(s) 246, 355, 611, 1973
BstAFI CTTAAG 2 cut(s) 754, 896
BstAPI GCANNNNNTGC 2 cut(s) 96, 2426
BstAUI TGTACA 3 cut(s) 965, 1088, 3859
BstBAI YACGTR 3 cut(s) 2444, 2885, 2933
BstDEI CTNAG 5 cut(s) 974, 2184, 2474, 3841, 4448
BstDSI CCRYGG 7 cut(s) 79, 367, 1121, 1325, 3149, 4757, 4862
BstENI CCTNNNNNAGG 1 cut(s) 2576
BstFNI CGCG 5 cut(s) 34, 352, 369, 380, 1327
BstH2I RGCGCY 2 cut(s) 218, 358
BstHHI GCGC 4 cut(s) 36, 217, 357, 723
BstMAI GTCTC 9 cut(s) 166, 253, 557, 1533, 2613, 3251, 4530, 4772, 4852
BstNI CCWGG 9 cut(s) 185, 3075, 3308, 3702, 3732, 3794, 4083, 4092, 4200
BstNSI RCATGY 2 cut(s) 2831, 4787
BstPAI GACNNNNGTC 1 cut(s) 1450
BstSFI CTRYAG 9 cut(s) 68, 801, 1059, 1250, 1497, 1579, 1936, 2976, 4419
BstSLI GKGCMC 4 cut(s) 426, 638, 1109, 1912
BstUI CGCG 5 cut(s) 34, 352, 369, 380, 1327
BstV2I GAAGAC 2 cut(s) 739, 2574
BstXI CCANNNNNNTGG 5 cut(s) 1002, 3150, 3272, 3339, 4098
BstZ17I GTATAC 2 cut(s) 1899, 3101
BsuI GTATCC 2 cut(s) 1411, 2936
BtgI CCRYGG 7 cut(s) 79, 367, 1121, 1325, 3149, 4757, 4862
BtgZI GCGATG 2 cut(s) 324, 1377
BtrI CACGTC 2 cut(s) 160, 1586
BtsI GCAGTG 1 cut(s) 77
BtsIMutI CAGTG 6 cut(s) 77, 1522, 2085, 2321, 2518, 3862
BveI ACCTGC 2 cut(s) 794, 3897
CciI TCATGA 2 cut(s) 2122, 2767
CfoI GCGC 4 cut(s) 36, 217, 357, 723
Cfr10I RCCGGY 4 cut(s) 403, 519, 1009, 1109
Cfr13I GGNCC 6 cut(s) 187, 401, 1763, 4196, 4298, 4707
Cfr42I CCGCGG 2 cut(s) 370, 1328
Cfr9I CCCGGG 1 cut(s) 2550
CseI GACGC 7 cut(s) 40, 235, 369, 600, 1981, 2066, 4789
CspAI ACCGGT 1 cut(s) 1109
DdeI CTNAG 5 cut(s) 974, 2184, 2474, 3841, 4448
DinI GGCGCC 1 cut(s) 356
DraI TTTAAA 3 cut(s) 2112, 2647, 3687
DraIII CACNNNGTG 2 cut(s) 529, 782
DriI GACNNNNNGTC 2 cut(s) 100, 672
EaeI YGGCCR 7 cut(s) 76, 1555, 1795, 2229, 2325, 3958, 4339
Eam1104I CTCTTC 6 cut(s) 42, 1572, 2040, 2131, 2550, 4512
Eam1105I GACNNNNNGTC 2 cut(s) 100, 672
EarI CTCTTC 6 cut(s) 42, 1572, 2040, 2131, 2550, 4512
EciI GGCGGA 5 cut(s) 106, 244, 463, 572, 1295
Eco130I CCWWGG 8 cut(s) 79, 146, 1121, 1217, 2858, 3149, 3236, 4862
Eco147I AGGCCT 1 cut(s) 341
Eco24I GRGCYC 2 cut(s) 1334, 4018
Eco31I GGTCTC 2 cut(s) 3251, 4852
Eco32I GATATC 1 cut(s) 3445
Eco47I GGWCC 1 cut(s) 1763
Eco57I CTGAAG 4 cut(s) 2586, 3175, 3396, 3499
Eco72I CACGTG 1 cut(s) 2885
Eco88I CYCGRG 3 cut(s) 428, 2550, 4029
EcoNI CCTNNNNNAGG 1 cut(s) 2576
EcoO109I RGGNCCY 2 cut(s) 187, 4707
EcoRI GAATTC 1 cut(s) 2999
EcoRII CCWGG 9 cut(s) 183, 3073, 3306, 3700, 3730, 3792, 4081, 4090, 4198
EcoRV GATATC 1 cut(s) 3445
EcoT14I CCWWGG 8 cut(s) 79, 146, 1121, 1217, 2858, 3149, 3236, 4862
EcoT22I ATGCAT 2 cut(s) 1075, 3247
EcoT38I GRGCYC 2 cut(s) 1334, 4018
EgeI GGCGCC 1 cut(s) 356
EheI GGCGCC 1 cut(s) 356
ErhI CCWWGG 8 cut(s) 79, 146, 1121, 1217, 2858, 3149, 3236, 4862
Esp3I CGTCTC 5 cut(s) 166, 253, 557, 1533, 4772
FalI AAGNNNNNCTT 6 cut(s) 83, 115, 4491, 4523, 4509, 4541
FaqI GGGAC 9 cut(s) 108, 679, 1749, 2414, 2705, 3026, 3591, 4736, 4738
FauI CCCGC 8 cut(s) 386, 494, 600, 1438, 1495, 1743, 2260, 4268
FauNDI CATATG 1 cut(s) 2671
FblI GTMKAC 3 cut(s) 1582, 1898, 3100
FriOI GRGCYC 2 cut(s) 1334, 4018
FspBI CTAG 7 cut(s) 147, 1218, 1292, 1341, 1682, 3237, 4044
GlaI GCGC 4 cut(s) 35, 216, 356, 722
GsaI CCCAGC 2 cut(s) 474, 2197
GsuI CTGGAG 8 cut(s) 211, 764, 1851, 2187, 2388, 2778, 2938, 3714
HaeII RGCGCY 2 cut(s) 218, 358
HgaI GACGC 7 cut(s) 40, 235, 369, 600, 1981, 2066, 4789
HhaI GCGC 4 cut(s) 36, 217, 357, 723
Hin1I GRCGYC 4 cut(s) 246, 355, 611, 1973
Hin6I GCGC 4 cut(s) 34, 215, 355, 721
HinP1I GCGC 4 cut(s) 34, 215, 355, 721
HindIII AAGCTT 4 cut(s) 1366, 3408, 3838, 4476
Hpy99I CGWCG 5 cut(s) 170, 173, 302, 338, 2060
HpyF3I CTNAG 5 cut(s) 974, 2184, 2474, 3841, 4448
Hsp92I GRCGYC 4 cut(s) 246, 355, 611, 1973
HspAI GCGC 4 cut(s) 34, 215, 355, 721
KasI GGCGCC 1 cut(s) 354
Kpn2I TCCGGA 1 cut(s) 192
KroI GCCGGC 1 cut(s) 519
KroNI GCCGGC 1 cut(s) 521
KspI CCGCGG 2 cut(s) 370, 1328
LguI GCTCTTC 2 cut(s) 1572, 2550
MaeI CTAG 7 cut(s) 147, 1218, 1292, 1341, 1682, 3237, 4044
MfeI CAATTG 1 cut(s) 2061
MhlI GDGCHC 7 cut(s) 426, 638, 1109, 1139, 1334, 1912, 4018
MlsI TGGCCA 5 cut(s) 1557, 2231, 2327, 3960, 4341
MluI ACGCGT 1 cut(s) 378
MluNI TGGCCA 5 cut(s) 1557, 2231, 2327, 3960, 4341
Mly113I GGCGCC 1 cut(s) 355
MlyI GAGTC 3 cut(s) 729, 1073, 3741
Mox20I TGGCCA 5 cut(s) 1557, 2231, 2327, 3960, 4341
Mph1103I ATGCAT 2 cut(s) 1075, 3247
MroI TCCGGA 1 cut(s) 192
MroNI GCCGGC 1 cut(s) 519
MroXI GAANNNNTTC 3 cut(s) 1031, 2544, 3939
MscI TGGCCA 5 cut(s) 1557, 2231, 2327, 3960, 4341
Msp20I TGGCCA 5 cut(s) 1557, 2231, 2327, 3960, 4341
MspA1I CMGCKG 5 cut(s) 61, 369, 1327, 1502, 2366
MspCI CTTAAG 2 cut(s) 754, 896
MunI CAATTG 1 cut(s) 2061
MvaI CCWGG 9 cut(s) 185, 3075, 3308, 3702, 3732, 3794, 4083, 4092, 4200
MvnI CGCG 5 cut(s) 34, 352, 369, 380, 1327
NaeI GCCGGC 1 cut(s) 521
NarI GGCGCC 1 cut(s) 355
NciI CCSGG 4 cut(s) 2551, 2552, 2946, 4426
NcoI CCATGG 4 cut(s) 79, 1121, 3149, 4862
NdeI CATATG 1 cut(s) 2671
NgoMIV GCCGGC 1 cut(s) 519
NheI GCTAGC 1 cut(s) 1291
NmuCI GTSAC 8 cut(s) 932, 1508, 2528, 3067, 3200, 3541, 3767, 4885
NsiI ATGCAT 2 cut(s) 1075, 3247
NspI RCATGY 2 cut(s) 2831, 4787
OliI CACNNNNGTG 3 cut(s) 1986, 3267, 3548
PaeI GCATGC 1 cut(s) 4787
PaeR7I CTCGAG 1 cut(s) 4029
PagI TCATGA 2 cut(s) 2122, 2767
PaqCI CACCTGC 2 cut(s) 794, 3897
PceI AGGCCT 1 cut(s) 341
PciSI GCTCTTC 2 cut(s) 1572, 2550
PcsI WCGNNNNNNNCGW 4 cut(s) 165, 177, 306, 919
PdiI GCCGGC 1 cut(s) 521
PdmI GAANNNNTTC 3 cut(s) 1031, 2544, 3939
PfeI GAWTC 7 cut(s) 220, 820, 1027, 1865, 4025, 4269, 4594
PflMI CCANNNNNTGG 7 cut(s) 1122, 1127, 2864, 2961, 3478, 3620, 4868
PfoI TCCNGGA 2 cut(s) 2944, 3730
PinAI ACCGGT 1 cut(s) 1109
PleI GAGTC 3 cut(s) 729, 1072, 3741
PluTI GGCGCC 1 cut(s) 358
PmaCI CACGTG 1 cut(s) 2885
PmlI CACGTG 1 cut(s) 2885
PpsI GAGTC 3 cut(s) 729, 1072, 3741
Ppu21I YACGTR 3 cut(s) 2444, 2885, 2933
PshAI GACNNNNGTC 1 cut(s) 1450
PshBI ATTAAT 1 cut(s) 2589
PsiI TTATAA 3 cut(s) 2379, 2798, 4040
Psp6I CCWGG 9 cut(s) 183, 3073, 3306, 3700, 3730, 3792, 4081, 4090, 4198
PspCI CACGTG 1 cut(s) 2885
PspFI CCCAGC 2 cut(s) 470, 2193
PspGI CCWGG 9 cut(s) 183, 3073, 3306, 3700, 3730, 3792, 4081, 4090, 4198
PspPI GGNCC 6 cut(s) 187, 401, 1763, 4196, 4298, 4707
PstI CTGCAG 6 cut(s) 72, 805, 1063, 1254, 1501, 2980
SacII CCGCGG 2 cut(s) 370, 1328
SapI GCTCTTC 2 cut(s) 1572, 2550
Sau96I GGNCC 6 cut(s) 187, 401, 1763, 4196, 4298, 4707
SchI GAGTC 3 cut(s) 729, 1073, 3741
SduI GDGCHC 7 cut(s) 426, 638, 1109, 1139, 1334, 1912, 4018
SfcI CTRYAG 9 cut(s) 68, 801, 1059, 1250, 1497, 1579, 1936, 2976, 4419
SfoI GGCGCC 1 cut(s) 356
Sfr274I CTCGAG 1 cut(s) 4029
Sfr303I CCGCGG 2 cut(s) 370, 1328
SgrBI CCGCGG 2 cut(s) 370, 1328
SinI GGWCC 1 cut(s) 1763
SlaI CTCGAG 1 cut(s) 4029
SmaI CCCGGG 1 cut(s) 2552
SmlI CTYRAG 5 cut(s) 754, 896, 940, 3878, 4029
SmoI CTYRAG 5 cut(s) 754, 896, 940, 3878, 4029
SpeI ACTAGT 1 cut(s) 4043
SphI GCATGC 1 cut(s) 4787
SseBI AGGCCT 1 cut(s) 341
SspDI GGCGCC 1 cut(s) 354
SspI AATATT 1 cut(s) 2619
SspMI CTAG 7 cut(s) 147, 1218, 1292, 1341, 1682, 3237, 4044
StuI AGGCCT 1 cut(s) 341
StyI CCWWGG 8 cut(s) 79, 146, 1121, 1217, 2858, 3149, 3236, 4862
TaqI TCGA 7 cut(s) 279, 922, 1171, 3802, 4030, 4378, 4601
TatI WGTACW 4 cut(s) 965, 1088, 1254, 3859
TfiI GAWTC 7 cut(s) 220, 820, 1027, 1865, 4025, 4269, 4594
TscAI CASTG 6 cut(s) 77, 1522, 2092, 2328, 2518, 3862
TseFI GTSAC 8 cut(s) 932, 1508, 2528, 3067, 3200, 3541, 3767, 4885
Tsp45I GTSAC 8 cut(s) 932, 1508, 2528, 3067, 3200, 3541, 3767, 4885
TspGWI ACGGA 8 cut(s) 154, 169, 308, 1154, 2799, 3447, 3887, 4676
TspMI CCCGGG 1 cut(s) 2550
TspRI CASTG 6 cut(s) 77, 1522, 2092, 2328, 2518, 3862
Van91I CCANNNNNTGG 7 cut(s) 1122, 1127, 2864, 2961, 3478, 3620, 4868
Vha464I CTTAAG 2 cut(s) 754, 896
VneI GTGCAC 4 cut(s) 422, 634, 1105, 1908
VpaK11BI GGWCC 1 cut(s) 1763
VspI ATTAAT 1 cut(s) 2589
XagI CCTNNNNNAGG 1 cut(s) 2576
XapI RAATTY 7 cut(s) 274, 1146, 1957, 2642, 2999, 3318, 3585
XceI RCATGY 2 cut(s) 2831, 4787
XcmI CCANNNNNNNNNTGG 9 cut(s) 841, 2865, 3269, 3621, 3813, 4002, 4409, 4578, 4764
XhoI CTCGAG 1 cut(s) 4029
XmaI CCCGGG 1 cut(s) 2550
XmaJI CCTAGG 3 cut(s) 146, 1217, 3236
XmiI GTMKAC 3 cut(s) 1582, 1898, 3100
XmnI GAANNNNTTC 3 cut(s) 1031, 2544, 3939
XspI CTAG 7 cut(s) 147, 1218, 1292, 1341, 1682, 3237, 4044
Zsp2I ATGCAT 2 cut(s) 1075, 3247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.