MD02G1282000.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010072
Physical Location & Seq
Forward (+)
33793481 .. 33808000
14520 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1282000.v1.1.491

Sequence Viewer

Length: 2160 bp
ATGAAGGTACCGTGCTGCTTGGTGTGCCAGACCCGCTACGACGAGGAGGAAAGAGTTCCGCTTTTGCTTCAGTGCGGCCATGGCTTCTGCAAGGACTGCTTGTCGAAGATGTTCTCTTCGTGTCCGGATACTACCCTCGTCTGCCCCCGCTGCCGCCACGTCACCGTCGTCGGGAACTCCGTTCAGGCCCTCCGCAAGAACTTTGCCGTACTCGCCTTGATTCACTCCAGCTCCAATGGTGTCTCGTCTGCCGCCGCCGCCAATTTCGATTACGATTACACGGACGACGAGGATGGGGACGACGATGAGGATGAGGATGGGGACCGACGGTGTGCTCGTGGGTCCCACACGTCGAGCTCGGGTGGGTGTGGACCGGCGATCGAGCTGGCGGTGCACCACGACTTGAAGTTGGTGCGGCGGATAGGGGAGGGGAGGCAGGCCGGGGTTCAAATGTGGACTGCTGTCATTGGAGGTGGAGGTGGAAGGTGTCGTCACCGGGTTGCGGTGAAGAAAGTGGCGGTGGCGGAGGAGACAAGTATGGATTGGGTGATGGGGCAGCTGGATAATTTGCGGTGGGCATCGATGTGGTGTAGGAATGTGTGCACATTTCATGGGGCTATGAAGAGTGAAGGCACCTTGTGTCTTGTTATGGATAAGTGTTATGGGTCGGTTCAGTCCGAGATGCAGCGCAATGAGGGCAGGCTGACTTTGGAGCAAATTCTAAGATATGGGGCTGACATTGCACGAGGAGTGGCTGAACTTCATGCGGCAGGTGTTGTTTGTATGAATCTAAAACCGTCCAATCTTCTTTTGGATGAAAGTGGTCATGCAGTAGTTTCTGATTATGGAGTTGCTGCAATTCTGAAGAAACCTTCCTGCCGGAAAGCTCGATTGGAGTGTGACACCTCAAGGATCCATTCTTGTATGGAGTGTACAATGCTCAGCCCCCACTATGCAGCTCCAGAGGCATGGGAGCCAGTGAAGAAGTTATTGAATCCATTTTGGGAGGATGCTATTGGTATTTCTGCTGAGTCAGATGCATGGAGTTTTGGTTGTACATTGGTAGAAATGTGCACTGGTTCCATTCCATGGGTTGGTTTAAGCACTGAGGAAATTTATCGGGCTGTAATCAAGGCTCGGAAACTACCTCCACAATATGCAAGTGTAGTAGGTGTTGGGATACCTAGAGAATTGTGGAAAATGATTGGCGAGTGCCTGCAGTTCAAGTCATCGAAAAGACCATCTTTTAATTCAATGCTAGCCACTTTTCTTCGCCATTTGCAGGAGATACCACGAAGCCCTCCTGCAAGTCCTGATAATGTTTTAGCTAAATTCTCTGGATCAAATGCGACGGAACCATCTCCTATATCCAAATCAGAAGTTTTTCAGGGTAACCCCACCCTTCTGCATAGACTTGTGTCTGAAGGGGATGTGCGCGGTGTTAGAGATCTGCTTCAAAAGGCTTCAGCAGGGAGTGATAACAGCACAATATTGTCTCTATTAGAAGCTCAAAATGCTGATGGCCAAACTGCTCTCCATTTGGCCTGTAGACGCGGTAGTGCAGAACTTGTTAATTCTATTTTGGAGTATCAAGAGGCGAATGTGGATGTCTTGGACAAAGATGGGGATCCTCCACTGGTTTTTGCATTAGTAGCTGGATCCCCAGAATGCGTTCATGCTCTCATTAACAGAGGTGCTAATGTGAGATCTAGGTTGCGAGAAGGATTTGGTCCCTCTGTTGCTCATGTCTGTGCCTATCATGGCCAACCTGATTGTATGCGTGAGTTACTAATGGCTGGAGCAGATCCTAATGCAGTGGATGAGGAAGGTGAATCTGTACTGCATAGAGCTGTTGCGAAGAAATATACAGATTGTGCCCTTGTTGTTCTGGAAAATGGGGGCTCTAGGTCAATGTGTGTTTTGAATTCAGAAAAGTTTACACCGTTGCACTTGTGTGTGGCAACATGGAATGTTGCTGTTGTTAGAAGGTGGGTGGAAGTTGCAACACCAGAAGAGATTGCTGATGCAATTGATAGACCAAGTCCAGTTGGCACTGCATTGTGTATGGCAGCTTCTCTAAAGAAAGATCATGAAATTGGGAGGGCACAGATTAAGAACTTTCCCTTGGTTAACATTTTATCTGGCCATGATGACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Pkinase zf-C3HC4 zf-RING_2 zf-RING_5

Protein Analysis

720

Amino Acids

78.17

Weight (kDa)

5.91

Isoelectric Point (pI)

50.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 5 - 52 4e-06 Ring finger domain
zf-RING_UBOX PF13445 6 - 49 6.5e-06 RING-type zinc-finger
PK_Tyr_Ser-Thr PF07714 135 - 421 6.3e-28 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 163 - 421 8.5e-30 Protein kinase domain
Ank_2 PF12796 469 - 570 9e-13 Ankyrin repeats (3 copies)
Ank PF00023 507 - 540 7.7e-06 Ankyrin repeat
Ank_5 PF13857 561 - 617 4.9e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 582 - 661 1.4e-09 Ankyrin repeats (3 copies)
Ank_4 PF13637 582 - 626 7.4e-07 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 761
Acc36I ACCTGC 1 cut(s) 761
Acc65I GGTACC 1 cut(s) 7
AccB1I GGYRCC 2 cut(s) 7, 632
AccB7I CCANNNNNTGG 2 cut(s) 1089, 1094
AccI GTMKAC 1 cut(s) 1549
AccII CGCG 2 cut(s) 1437, 1554
AccIII TCCGGA 1 cut(s) 124
AclWI GGATC 8 cut(s) 907, 920, 1348, 1622, 1635, 1653, 1666, 1799
AcoI YGGCCR 4 cut(s) 76, 1522, 1762, 2143
AcsI RAATTY 4 cut(s) 717, 1113, 1331, 1924
AcuI CTGAAG 4 cut(s) 53, 884, 1443, 1449
AdeI CACNNNGTG 1 cut(s) 639
AfaI GTAC 5 cut(s) 9, 210, 934, 1057, 1839
AfiI CCNNNNNNNGG 5 cut(s) 171, 502, 1089, 1094, 1282
AflIII ACRYGT 1 cut(s) 348
AgsI TTSAA 7 cut(s) 406, 449, 994, 1225, 1254, 1457, 1924
AhdI GACNNNNNGTC 1 cut(s) 100
AjiI CACGTC 2 cut(s) 160, 351
AjuI GAANNNNNNNTTGG 4 cut(s) 875, 907, 2108, 2140
AleI CACNNNNGTG 1 cut(s) 1953
Alw21I GWGCWC 5 cut(s) 337, 359, 396, 605, 1076
Alw26I GTCTC 3 cut(s) 247, 524, 1500
Alw44I GTGCAC 3 cut(s) 392, 601, 1072
AlwI GGATC 8 cut(s) 907, 920, 1348, 1622, 1635, 1653, 1666, 1799
Ama87I CYCGRG 1 cut(s) 358
Aor13HI TCCGGA 1 cut(s) 124
AoxI GGCC 7 cut(s) 76, 186, 438, 1522, 1542, 1762, 2143
ApaLI GTGCAC 3 cut(s) 392, 601, 1072
ApeKI GCWGC 7 cut(s) 15, 150, 556, 685, 854, 956, 2069
ApoI RAATTY 4 cut(s) 717, 1113, 1331, 1924
Asp700I GAANNNNTTC 4 cut(s) 54, 110, 1383, 1671
Asp718I GGTACC 1 cut(s) 7
AspLEI GCGC 2 cut(s) 690, 1437
AspS9I GGNCC 5 cut(s) 187, 322, 342, 371, 1730
AsuC2I CCSGG 2 cut(s) 442, 497
AsuHPI GGTGA 5 cut(s) 154, 485, 517, 559, 1841
AsuNHI GCTAGC 1 cut(s) 1258
AvaI CYCGRG 1 cut(s) 358
AvaII GGWCC 4 cut(s) 322, 342, 371, 1730
BaeGI GKGCMC 5 cut(s) 396, 605, 1076, 1879, 2107
BalI TGGCCA 3 cut(s) 1524, 1764, 2145
BamHI GGATCC 3 cut(s) 912, 1627, 1658
BanI GGYRCC 2 cut(s) 7, 632
BanII GRGCYC 2 cut(s) 359, 1904
BarI GAAGNNNNNNTAC 2 cut(s) 2056, 2088
BauI CACGAG 2 cut(s) 336, 744
Bbv12I GWGCWC 5 cut(s) 337, 359, 396, 605, 1076
BbvI GCAGC 7 cut(s) 2, 137, 568, 697, 841, 968, 2081
BccI CCATC 7 cut(s) 287, 311, 544, 1249, 1366, 1514, 1616
BceAI ACGGC 1 cut(s) 191
BciVI GTATCC 2 cut(s) 121, 1173
BcnI CCSGG 2 cut(s) 442, 497
BcoDI GTCTC 3 cut(s) 247, 524, 1500
BfaI CTAG 4 cut(s) 1185, 1259, 1710, 1905
BfmI CTRYAG 2 cut(s) 1217, 1546
BfuAI ACCTGC 1 cut(s) 761
BfuI GTATCC 2 cut(s) 121, 1173
BglII AGATCT 2 cut(s) 1447, 1706
BlpI GCTNAGC 1 cut(s) 941
Bme1390I CCNGG 2 cut(s) 442, 497
Bme18I GGWCC 4 cut(s) 322, 342, 371, 1730
BmeRI GACNNNNNGTC 1 cut(s) 100
BmeT110I CYCGRG 1 cut(s) 358
BmgBI CACGTC 2 cut(s) 160, 351
BmgT120I GGNCC 5 cut(s) 187, 322, 342, 371, 1730
BmrFI CCNGG 2 cut(s) 442, 497
BmsI GCATC 5 cut(s) 587, 672, 1000, 1027, 2014
BmtI GCTAGC 1 cut(s) 1262
BoxI GACNNNNGTC 2 cut(s) 461, 1417
BpmI CTGGAG 3 cut(s) 211, 945, 1818
Bpu1102I GCTNAGC 1 cut(s) 941
BpuEI CTTGAG 1 cut(s) 892
BpuMI CCSGG 2 cut(s) 442, 497
Bsa29I ATCGAT 1 cut(s) 581
BsaBI GATNNNNATC 1 cut(s) 1626
BsaJI CCNNGG 4 cut(s) 79, 441, 1088, 2124
BsaWI WCCGGW 1 cut(s) 124
BsaXI ACNNNNNCTCC 4 cut(s) 215, 245, 1036, 1066
Bsc4I CCNNNNNNNGG 5 cut(s) 171, 502, 1089, 1094, 1282
Bse118I RCCGGY 1 cut(s) 373
Bse1I ACTGG 4 cut(s) 977, 1081, 1641, 2045
Bse3DI GCAATG 2 cut(s) 697, 738
Bse8I GATNNNNATC 1 cut(s) 1626
BseAI TCCGGA 1 cut(s) 124
BseCI ATCGAT 1 cut(s) 581
BseDI CCNNGG 4 cut(s) 79, 441, 1088, 2124
BseGI GGATG 8 cut(s) 298, 316, 322, 820, 1015, 1435, 1612, 1825
BseJI GATNNNNATC 1 cut(s) 1626
BseLI CCNNNNNNNGG 5 cut(s) 171, 502, 1089, 1094, 1282
BseMI GCAATG 2 cut(s) 697, 738
BseMII CTCAG 3 cut(s) 955, 1020, 1098
BseNI ACTGG 4 cut(s) 977, 1081, 1641, 2045
BseRI GAGGAG 3 cut(s) 59, 542, 762
BseSI GKGCMC 5 cut(s) 396, 605, 1076, 1879, 2107
BseXI GCAGC 7 cut(s) 2, 137, 568, 697, 841, 968, 2081
BsgI GTGCAG 1 cut(s) 1581
Bsh1236I CGCG 2 cut(s) 1437, 1554
Bsh1285I CGRYCG 1 cut(s) 381
BshFI GGCC 7 cut(s) 78, 188, 440, 1524, 1544, 1764, 2145
BshNI GGYRCC 2 cut(s) 7, 632
BshVI ATCGAT 1 cut(s) 581
BsiEI CGRYCG 1 cut(s) 381
BsiHKAI GWGCWC 5 cut(s) 337, 359, 396, 605, 1076
BsiHKCI CYCGRG 1 cut(s) 358
BsiSI CCGG 5 cut(s) 125, 374, 441, 496, 880
BslFI GGGAC 4 cut(s) 311, 328, 335, 1716
BslI CCNNNNNNNGG 5 cut(s) 171, 502, 1089, 1094, 1282
BsmAI GTCTC 3 cut(s) 247, 524, 1500
BsmFI GGGAC 4 cut(s) 311, 328, 335, 1716
BsmI GAATGC 1 cut(s) 1673
BsnI GGCC 7 cut(s) 78, 188, 440, 1524, 1544, 1764, 2145
BsoBI CYCGRG 1 cut(s) 358
Bsp1286I GDGCHC 8 cut(s) 337, 359, 396, 605, 1076, 1879, 1904, 2107
Bsp13I TCCGGA 1 cut(s) 124
Bsp1407I TGTACA 2 cut(s) 932, 1055
Bsp143I GATC 9 cut(s) 378, 912, 1340, 1447, 1627, 1658, 1706, 1804, 2086
Bsp1720I GCTNAGC 1 cut(s) 941
Bsp19I CCATGG 2 cut(s) 79, 1088
BspANI GGCC 7 cut(s) 78, 188, 440, 1524, 1544, 1764, 2145
BspCNI CTCAG 3 cut(s) 954, 1021, 1099
BspDI ATCGAT 1 cut(s) 581
BspEI TCCGGA 1 cut(s) 124
BspFNI CGCG 2 cut(s) 1437, 1554
BspHI TCATGA 1 cut(s) 2089
BspMAI CTGCAG 1 cut(s) 1221
BspMI ACCTGC 1 cut(s) 761
BspOI GCTAGC 1 cut(s) 1262
BspPI GGATC 8 cut(s) 907, 920, 1348, 1622, 1635, 1653, 1666, 1799
BspT107I GGYRCC 2 cut(s) 7, 632
BsrDI GCAATG 2 cut(s) 697, 738
BsrFI RCCGGY 1 cut(s) 373
BsrGI TGTACA 2 cut(s) 932, 1055
BsrI ACTGG 4 cut(s) 977, 1081, 1641, 2045
BssAI RCCGGY 1 cut(s) 373
BssECI CCNNGG 4 cut(s) 79, 441, 1088, 2124
BssMI GATC 9 cut(s) 378, 912, 1340, 1447, 1627, 1658, 1706, 1804, 2086
BssSI CACGAG 2 cut(s) 336, 744
BssT1I CCWWGG 3 cut(s) 79, 1088, 2124
Bst2BI CACGAG 2 cut(s) 336, 744
Bst4CI ACNGT 5 cut(s) 12, 166, 330, 798, 1944
Bst6I CTCTTC 3 cut(s) 121, 617, 2007
BstAPI GCANNNNNTGC 1 cut(s) 96
BstAUI TGTACA 2 cut(s) 932, 1055
BstC8I GCNNGC 5 cut(s) 387, 438, 701, 1217, 1260
BstDEI CTNAG 4 cut(s) 722, 941, 1029, 1107
BstDSI CCRYGG 2 cut(s) 79, 1088
BstEII GGTNACC 1 cut(s) 1391
BstF5I GGATG 8 cut(s) 298, 316, 322, 820, 1015, 1435, 1612, 1825
BstFNI CGCG 2 cut(s) 1437, 1554
BstHHI GCGC 2 cut(s) 690, 1437
BstKTI GATC 9 cut(s) 381, 915, 1343, 1450, 1630, 1661, 1709, 1807, 2089
BstMAI GTCTC 3 cut(s) 247, 524, 1500
BstMBI GATC 9 cut(s) 378, 912, 1340, 1447, 1627, 1658, 1706, 1804, 2086
BstMCI CGRYCG 1 cut(s) 381
BstPAI GACNNNNGTC 2 cut(s) 461, 1417
BstPI GGTNACC 1 cut(s) 1391
BstSCI CCNGG 2 cut(s) 440, 495
BstSFI CTRYAG 2 cut(s) 1217, 1546
BstSLI GKGCMC 5 cut(s) 396, 605, 1076, 1879, 2107
BstUI CGCG 2 cut(s) 1437, 1554
BstV1I GCAGC 7 cut(s) 2, 137, 568, 697, 841, 968, 2081
BstX2I RGATCY 6 cut(s) 912, 1447, 1627, 1658, 1706, 1804
BstXI CCANNNNNNTGG 1 cut(s) 969
BstYI RGATCY 6 cut(s) 912, 1447, 1627, 1658, 1706, 1804
Bsu15I ATCGAT 1 cut(s) 581
BsuI GTATCC 2 cut(s) 121, 1173
BsuRI GGCC 7 cut(s) 78, 188, 440, 1524, 1544, 1764, 2145
BsuTUI ATCGAT 1 cut(s) 581
BtgI CCRYGG 2 cut(s) 79, 1088
BtrI CACGTC 2 cut(s) 160, 351
BtsCI GGATG 8 cut(s) 298, 316, 322, 820, 1015, 1435, 1612, 1825
BtsI GCAGTG 2 cut(s) 1821, 2052
BtsIMutI CAGTG 7 cut(s) 77, 984, 1074, 1104, 1634, 1821, 2052
BveI ACCTGC 1 cut(s) 761
Cac8I GCNNGC 5 cut(s) 387, 438, 701, 1217, 1260
CciI TCATGA 1 cut(s) 2089
CfoI GCGC 2 cut(s) 690, 1437
Cfr10I RCCGGY 1 cut(s) 373
Cfr13I GGNCC 5 cut(s) 187, 322, 342, 371, 1730
ClaI ATCGAT 1 cut(s) 581
CseI GACGC 1 cut(s) 1560
Csp6I GTAC 5 cut(s) 8, 209, 933, 1056, 1838
CviQI GTAC 5 cut(s) 8, 209, 933, 1056, 1838
DdeI CTNAG 4 cut(s) 722, 941, 1029, 1107
DpnI GATC 9 cut(s) 380, 914, 1342, 1449, 1629, 1660, 1708, 1806, 2088
DpnII GATC 9 cut(s) 378, 912, 1340, 1447, 1627, 1658, 1706, 1804, 2086
DraIII CACNNNGTG 1 cut(s) 639
DriI GACNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 4 cut(s) 76, 1522, 1762, 2143
Eam1104I CTCTTC 3 cut(s) 121, 617, 2007
Eam1105I GACNNNNNGTC 1 cut(s) 100
EarI CTCTTC 3 cut(s) 121, 617, 2007
EciI GGCGGA 2 cut(s) 433, 539
Ecl136II GAGCTC 1 cut(s) 357
Eco130I CCWWGG 3 cut(s) 79, 1088, 2124
Eco24I GRGCYC 2 cut(s) 359, 1904
Eco47I GGWCC 4 cut(s) 322, 342, 371, 1730
Eco53kI GAGCTC 1 cut(s) 357
Eco57I CTGAAG 4 cut(s) 53, 884, 1443, 1449
Eco88I CYCGRG 1 cut(s) 358
Eco91I GGTNACC 1 cut(s) 1391
EcoICRI GAGCTC 1 cut(s) 357
EcoO109I RGGNCCY 2 cut(s) 187, 342
EcoO65I GGTNACC 1 cut(s) 1391
EcoRI GAATTC 1 cut(s) 1924
EcoT14I CCWWGG 3 cut(s) 79, 1088, 2124
EcoT22I ATGCAT 1 cut(s) 1042
EcoT38I GRGCYC 2 cut(s) 359, 1904
ErhI CCWWGG 3 cut(s) 79, 1088, 2124
FalI AAGNNNNNCTT 4 cut(s) 83, 115, 1228, 1260
FaqI GGGAC 4 cut(s) 311, 328, 335, 1716
FauI CCCGC 2 cut(s) 41, 155
FblI GTMKAC 1 cut(s) 1549
FokI GGATG 8 cut(s) 305, 323, 329, 827, 1022, 1442, 1619, 1832
FriOI GRGCYC 2 cut(s) 359, 1904
FspBI CTAG 4 cut(s) 1185, 1259, 1710, 1905
GlaI GCGC 2 cut(s) 689, 1436
GsuI CTGGAG 3 cut(s) 211, 945, 1818
HaeIII GGCC 7 cut(s) 78, 188, 440, 1524, 1544, 1764, 2145
HapII CCGG 5 cut(s) 125, 374, 441, 496, 880
HgaI GACGC 1 cut(s) 1560
HhaI GCGC 2 cut(s) 690, 1437
Hin6I GCGC 2 cut(s) 688, 1435
HinP1I GCGC 2 cut(s) 688, 1435
HincII GTYRAC 1 cut(s) 2131
HindII GTYRAC 1 cut(s) 2131
HinfI GANTC 5 cut(s) 220, 787, 994, 1031, 1832
HpaI GTTAAC 1 cut(s) 2131
HpaII CCGG 5 cut(s) 125, 374, 441, 496, 880
HphI GGTGA 5 cut(s) 154, 485, 517, 559, 1841
Hpy166II GTNNAC 9 cut(s) 371, 394, 456, 603, 933, 1074, 1550, 1938, 2131
Hpy188I TCNGA 8 cut(s) 679, 841, 864, 1036, 1140, 1378, 1423, 1930
Hpy188III TCNNGA 8 cut(s) 125, 172, 962, 1313, 1338, 1592, 1889, 2090
Hpy8I GTNNAC 9 cut(s) 371, 394, 456, 603, 933, 1074, 1550, 1938, 2131
Hpy99I CGWCG 8 cut(s) 44, 170, 173, 290, 305, 330, 355, 1354
HpyAV CCTTC 8 cut(s) 477, 623, 882, 1412, 1418, 1715, 1820, 1980
HpyCH4III ACNGT 5 cut(s) 12, 166, 330, 798, 1944
HpyCH4IV ACGT 2 cut(s) 159, 350
HpyF3I CTNAG 4 cut(s) 722, 941, 1029, 1107
HpySE526I ACGT 2 cut(s) 159, 350
HspAI GCGC 2 cut(s) 688, 1435
KflI GGGWCCC 1 cut(s) 342
Kpn2I TCCGGA 1 cut(s) 124
KpnI GGTACC 1 cut(s) 11
KspAI GTTAAC 1 cut(s) 2131
Kzo9I GATC 9 cut(s) 378, 912, 1340, 1447, 1627, 1658, 1706, 1804, 2086
LmnI GCTCC 5 cut(s) 236, 712, 964, 973, 1799
Lsp1109I GCAGC 7 cut(s) 2, 137, 568, 697, 841, 968, 2081
LweI GCATC 5 cut(s) 587, 672, 1000, 1027, 2014
MaeI CTAG 4 cut(s) 1185, 1259, 1710, 1905
MaeII ACGT 2 cut(s) 159, 350
MaeIII GTNAC 5 cut(s) 160, 491, 899, 1391, 1785
MalI GATC 9 cut(s) 380, 914, 1342, 1449, 1629, 1660, 1708, 1806, 2088
MboI GATC 9 cut(s) 378, 912, 1340, 1447, 1627, 1658, 1706, 1804, 2086
MfeI CAATTG 1 cut(s) 2028
MflI RGATCY 6 cut(s) 912, 1447, 1627, 1658, 1706, 1804
MhlI GDGCHC 8 cut(s) 337, 359, 396, 605, 1076, 1879, 1904, 2107
MlsI TGGCCA 3 cut(s) 1524, 1764, 2145
MluNI TGGCCA 3 cut(s) 1524, 1764, 2145
MlyI GAGTC 1 cut(s) 1040
Mox20I TGGCCA 3 cut(s) 1524, 1764, 2145
Mph1103I ATGCAT 1 cut(s) 1042
MroI TCCGGA 1 cut(s) 124
MroXI GAANNNNTTC 4 cut(s) 54, 110, 1383, 1671
MscI TGGCCA 3 cut(s) 1524, 1764, 2145
MseI TTAA 6 cut(s) 1100, 1248, 1572, 1686, 2112, 2130
MslI CAYNNNNRTG 3 cut(s) 583, 1749, 1953
Msp20I TGGCCA 3 cut(s) 1524, 1764, 2145
MspA1I CMGCKG 2 cut(s) 150, 559
MspI CCGG 5 cut(s) 125, 374, 441, 496, 880
MspR9I CCNGG 2 cut(s) 442, 497
MunI CAATTG 1 cut(s) 2028
Mva1269I GAATGC 1 cut(s) 1673
MvnI CGCG 2 cut(s) 1437, 1554
NciI CCSGG 2 cut(s) 442, 497
NcoI CCATGG 2 cut(s) 79, 1088
NdeII GATC 9 cut(s) 378, 912, 1340, 1447, 1627, 1658, 1706, 1804, 2086
NheI GCTAGC 1 cut(s) 1258
NmuCI GTSAC 3 cut(s) 160, 491, 899
NsiI ATGCAT 1 cut(s) 1042
OliI CACNNNNGTG 1 cut(s) 1953
PagI TCATGA 1 cut(s) 2089
PaqCI CACCTGC 1 cut(s) 761
PcsI WCGNNNNNNNCGW 3 cut(s) 165, 177, 334
PctI GAATGC 1 cut(s) 1673
PdmI GAANNNNTTC 4 cut(s) 54, 110, 1383, 1671
PfeI GAWTC 4 cut(s) 220, 787, 994, 1832
PflFI GACNNNGTC 1 cut(s) 2040
PflMI CCANNNNNTGG 2 cut(s) 1089, 1094
Ple19I CGATCG 1 cut(s) 381
PleI GAGTC 1 cut(s) 1039
PpsI GAGTC 1 cut(s) 1039
PpuMI RGGWCCY 1 cut(s) 342
PshAI GACNNNNGTC 2 cut(s) 461, 1417
Psp124BI GAGCTC 1 cut(s) 359
Psp5II RGGWCCY 1 cut(s) 342
PspEI GGTNACC 1 cut(s) 1391
PspPI GGNCC 5 cut(s) 187, 322, 342, 371, 1730
PspPPI RGGWCCY 1 cut(s) 342
PstI CTGCAG 1 cut(s) 1221
PsuI RGATCY 6 cut(s) 912, 1447, 1627, 1658, 1706, 1804
PsyI GACNNNGTC 1 cut(s) 2040
PvuI CGATCG 1 cut(s) 381
PvuII CAGCTG 1 cut(s) 559
RsaI GTAC 5 cut(s) 9, 210, 934, 1057, 1839
RsaNI GTAC 5 cut(s) 8, 209, 933, 1056, 1838
RseI CAYNNNNRTG 3 cut(s) 583, 1749, 1953
SacI GAGCTC 1 cut(s) 359
SaqAI TTAA 6 cut(s) 1100, 1248, 1572, 1686, 2112, 2130
Sau3AI GATC 9 cut(s) 378, 912, 1340, 1447, 1627, 1658, 1706, 1804, 2086
Sau96I GGNCC 5 cut(s) 187, 322, 342, 371, 1730
SchI GAGTC 1 cut(s) 1040
ScrFI CCNGG 2 cut(s) 442, 497
SduI GDGCHC 8 cut(s) 337, 359, 396, 605, 1076, 1879, 1904, 2107
SfaNI GCATC 5 cut(s) 587, 672, 1000, 1027, 2014
SfcI CTRYAG 2 cut(s) 1217, 1546
SinI GGWCC 4 cut(s) 322, 342, 371, 1730
SmiMI CAYNNNNRTG 3 cut(s) 583, 1749, 1953
SmlI CTYRAG 1 cut(s) 907
SmoI CTYRAG 1 cut(s) 907
SspI AATATT 1 cut(s) 1491
SspMI CTAG 4 cut(s) 1185, 1259, 1710, 1905
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 2 cut(s) 440, 495
StyI CCWWGG 3 cut(s) 79, 1088, 2124
TaaI ACNGT 5 cut(s) 12, 166, 330, 798, 1944
TaiI ACGT 2 cut(s) 162, 353
TaqI TCGA 7 cut(s) 104, 267, 353, 381, 581, 889, 1232
TaqII GACCGA 1 cut(s) 339
TatI WGTACW 3 cut(s) 932, 1055, 1837
TauI GCSGC 7 cut(s) 78, 156, 254, 257, 260, 418, 770
TfiI GAWTC 4 cut(s) 220, 787, 994, 1832
Tru1I TTAA 6 cut(s) 1100, 1248, 1572, 1686, 2112, 2130
Tru9I TTAA 6 cut(s) 1100, 1248, 1572, 1686, 2112, 2130
TscAI CASTG 7 cut(s) 77, 984, 1081, 1111, 1641, 1821, 2059
TseFI GTSAC 3 cut(s) 160, 491, 899
TseI GCWGC 7 cut(s) 15, 150, 556, 685, 854, 956, 2069
Tsp45I GTSAC 3 cut(s) 160, 491, 899
TspDTI ATGAA 8 cut(s) 17, 599, 635, 752, 800, 831, 1664, 2106
TspGWI ACGGA 3 cut(s) 169, 296, 1367
TspRI CASTG 7 cut(s) 77, 984, 1081, 1111, 1641, 1821, 2059
Tth111I GACNNNGTC 1 cut(s) 2040
Van91I CCANNNNNTGG 2 cut(s) 1089, 1094
VneI GTGCAC 3 cut(s) 392, 601, 1072
VpaK11BI GGWCC 4 cut(s) 322, 342, 371, 1730
XapI RAATTY 4 cut(s) 717, 1113, 1331, 1924
XcmI CCANNNNNNNNNTGG 1 cut(s) 808
XmiI GTMKAC 1 cut(s) 1549
XmnI GAANNNNTTC 4 cut(s) 54, 110, 1383, 1671
XspI CTAG 4 cut(s) 1185, 1259, 1710, 1905
Zsp2I ATGCAT 1 cut(s) 1042
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.