MD07G1048200.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010072
Physical Location & Seq
Forward (+)
4124765 .. 4137544
12780 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1048200.v1.1.491

Sequence Viewer

Length: 4884 bp
ATGAAGGTCCCGTGCTGCTCCGTCTGCCAGACCCGCTACGACGAGGAGGAAAGAGTTCCGCTTTTGCTTCAGTGCGGCCATGGCTTCTGCAAGGACTGCTTGTCCAAAATGTTCTCTTCCTGTCCCGATACTACCCTCGTCTGCCCCCGCTGTCGCCACGTCTCCGTCGTCGGGAACTCCGTCCAGGCGCTGAGGAAGAACTTCGCCGTGCTCGCCCTGATTCACTCCAGCTCCAATGCTGTCTCGTCTGCTGCCGCTGCCAATTTCGATTGCGATTACACGGACGACGAGGATGGGGACGATGATGAGGATGACGATGGGGACCGAAGGTGTGCTCGTGGGTCCCACACGTCGATCTCGGGCGGGTGCGGGCCGGTGATCGAGCTGGCGGTGCACCCTGACTTGAAGTTGGTGCGGCGGATAGGGGAGGGCAGGCAGGCTGGGGTTCAAATGTGGACTGCCGTCATCGGAGGTGGAGGAGGGAGGTGCCGCCACCGGGTTGCGGTGAAGAAAGTGGCAGTAGCGGAGGAGACGAGTATGGATTGGGTGATGGGGCAGCTGGATAATTTGCGGCGGGCATCGATGTGGTGTAGGAATGTGTGCACATTTCATGGGGCTATTAAGAGTGAAGGCACCTTGTGTCTTGTTATGGATAAGTGTTATGGATCGGTTCAGTCCGAGATGCAGCGCAATGAGGGCAGGCTTACTTTGGAGCAAATTCTAAGGAAATTCTCGTGGATATACGGGGCTGACATTGCGCGAGGAGTAGCTGAACTTCATGCAGCAGGTGTTGTTTGTATGAATCTAAAACCGTCCAATCTTCTTTTGGATGAAAGTGGTCACGCGGTAGTTTCTGATTATGGAGTTGCTGCAATTCTGAAGAAACCTTCCTGCCGGAAAGCTCGATTGGAGTGTGACACCTCAAGGATCCATTCTTGTATGGAGTGTACGATGCTCAGCCCCCACTATGCAGCTCCAGAGGCATGGGAACCAGTGAAGAAGTTACTGAATCCATTTTGGGAGGATGCTATCGGTATTTCTGCAGAGTCAGATGCATGGAGTTTTGGTTGTACATTGGTAGAAATGTGCACTGGTTCCATTCCATGGGCTGGTTTAAGCACTGAGGAAATTTATCGGGCTGTAATCAAGACTCGGAAACTACCTCCACAATATGCAAGTGTAGTAGGTGTTGGAATTCCTAGAGAATTGTGGAAAATGATTGGCGAGTGCCTGCAGTTCAAGGCATCAAAAAGACCATCTTTTAATTCAATGCTAGCCACTTTTCTTCGCCATTTGCAGGAGATACCACGCAGCCCTCCTGCAAGTCCTGATAATGTTTTAGCTAAATGCTCTGGATCAAATGTGATGGAACCATCTCCTGTATCTGAATCAGAAGTTTTTCAGGGTAACCCCACTCTTCTGCATAGACTTGTGTCTGAAGGGGATGTGCGCAGTGTTAGAGATCTGCTTCAAAAGGCTTCAGCAGGGAGTGATAACAGTACAATATTGTCTCTATTAGAAGCTCAAAATGCTGATGGCCAAACTGCTCTCCATTTGGCCTGTAGACGTGGCAGTGCAGAACTTGTTAATGCTATTTTGGAGTATCAAGAGGCAAATGTGGATGTCTTGGACAAAGATGGGGATCCTCCACTAGTTTTTGCATTAGTAGCTGGATCCCCAGAATGCGTTCATGCTCTCATTAACAGAGGTGCTAATGTGAGATCTAGGTTGCGAGAAGGCTTTGGTCCCTCAGTTGCTCATGTCTGTGCCTATCATGGCCAACCTGATTGTATGCGTGAGTTACTAATGGCTGGAGCGGACCCTAATGCGGTGGATGAGGAAGGTGAATCTGTACTGCATAGAGCTGTTGCAAAGAAATATACAGATTGTGCCCTTGTTGTTCTGGAAAATGGGGGCTCTAGGTCAATGTGTGTTTTGAATTCAGAAAAGTTTACACCATTGCACTTGTGTGTGGCAACATGGAATGTTGCTGTTGTTAGAAGGTGGGTGGAAGTTGCAACACCAGAAGAGATTGCTGATGCAATTGATATACCAAGTTCAGTTGGCACTGCATTGTGTATGGCAGCTTCTCTAAAGAAAGATCATGAAATTGCCAAAGGAAGAGAAATGGTGCAAATTTTGCTAGCTTCTGGAGCAGATCCAACTGCTCAAGATTTGCAGCATGGACGGACAGCTTTGCATACAGCTTCAATGGCCAATGAAGTTGAGTTGGTCAAGATTATACTTGATGCTGGAGTTGATGTGAACATTCAGAATGTACAAAATACAATTCCTCTTCATGTAGCACTGGCCAGAGGGGCAAAGTCATGTGTTGGACTGCTTTTATCTGCTGGAGCAAATTATAATTTGCAGGATGATGAAGGCGACAATGCTTTCCATATAGCAGCAGATGCAGCAAAAATGATACGTGAGAATCTCGAATGGCTTATTGTCATGCTTAGAAATCCTGATGCTGCTGTTGAAGCTAGGAATCACAGTGGCAAGACCTTACGTGACTTTTTGGAGGCCCTTCCTCGGGAATGGATATCTGAAGATCTTATGGAGGCACTTGTAAATCGGGGAGTGCATTTGTCTCCTACAACATTTGATGTGGGAGACTGGGTCAAATTTAAAAGAAGTATAACAACTCCCACGTATGGATGGCAAGGTGCAAAACATAGAAGTGTTGGCTTTGTGCAAGGTGCCCCAGATAAGGACCATTTGATAGTATCATTTTGTTCTGGAGAGGCTCGTGTTTTGGCGAATGAAGTTGTAAAAGTTATTCCCTTGGACAGGGGACAGCATGTTCAGCTGAAACCAGATGTCAAAGAGCCCAGATTTGGGTGGCGTGGCCAGTCACGTGATAGCATTGGTACTGTATTATGTGTTGATGATGATGGTATCCTACGTGTTGGCTTTCCCGGAGCCTCCAGAGGGTGGAAAGCTGATCCAGCAGAGATGGAGAGAGTTGAAGAATTCAAGGTTGGGGATTGGGTTCGAATTCGCCCCACTCTTACAACTGCAAAGCACGGATTAGGATCTGTTACACCTGGTAGCATTGGTATTGTGTATTGTATAAGACCAGATAGTAGTCTGTTGCTGGAATTAAGCTATCTTCCAAGTCCATGGCATTGTGAACCAGAGGAGGTTGAGCCTGTAACCCCTTTCAGGATTGGTGACAGGGTATGTGTTAAGCGATCTGTTGCAGAGCCTAGGTATGCATGGGGTGGTGAGACGCACCATAGTGTTGGAAAAATAAGTGAGATAGAGAATGACGGGCTCCTGGTAATTGAAATTCCAAATCGACCAATACCATGGCAGGCAGATCCTTCTGACATGGAGAAGGTGGAAGATTTTAAGGTAGGGGACTGGGTTAGAGTCAAAGCTTCTGTACCATCCCCCAAATACGGATGGGAGGATATCACAAGGAACAGTGTTGGGATAATTCATAGCTTGGAGGAAGATGGTGATATGGGTGTCGCCTTCTGCTTCAGGAGCAAGCCTTTTTCTTGTTCTGTCACGGATGTGGAGAAGGTGCCCCCTTTTGAAGTGGGGCAAGAGATTCATGTGACGTCATCTATTTCTCAGCCAAGACTTGGATGGTCAAATGAAAGTCCAGCAACTGTTGGAAAAATTGTTAGGATTGACATGGATGGAGCTTTGAATACAAAAGTCACTGGCCGACAGAGCTTGTGGAAAGTTTCTCCTGGAGATGCGGAACGACTCTCTGGATTTGAAGTTGGTGACTGGGTACGTTCAAAACCAAGCCTGGGAACTCGACCAAGTTATGACTGGAACAGCATTGGAAAGGAAAGTTTAGCTGTCGTGCACAGTGTACAAGATACAGGTTATCTGGAGTTGGCTTGTTGTTTCCGTAAAGGCAGGTGGATAACTCATTACACAGATGTTGAAAAGGTTCCATGCTTTAAAATTGGGCAGTATGTCCGGTTCCGGATTGGACTTGTTGAACCAAGATGGGGCTGGAGAGGGGCTCAACTTGATTCTCGAGGCGTCATAACTAGTGTTCATGCTGATGGGGAAGTGAGAGTGGCTTTCTCTGGTTTGCCAGGATTGTGGAGAGGAGATCCTGCTGATCTTGAAATAGAGCAGATTTTTGAAGTTGGAGAGTGGGTGAGATTAAAAGATCAAGCTGGTGCTTGGAAATCCATTGGGCCAGGCAGTGTTGGAGTGGTGCAAGGATTGGGATACGATGCAGATAAATGGGATGGAACCACTTCCGTTGGATTTTGTGGGGAGCAAGAAAAATGGGTTGGGCCTACCTCTGCACTCGAAAGGGTGAACAGGCTCATGGTTGGCCAGAAGGTTAGAGTTAAACTGTCTGTCAAGCAGCCAAGATTTGGGTGGTCAGGCCACAGCCATGCAAGTCTTGGAACCATATCCACAATTGATGCTGATGGCAAGCTGAGAATATACACTCCAGCTGGTTCAAAAGCTTGGATGCTGGATCCATCAGAAGTGGAACTTGTGGAAGAAGAGGAGCTTCACATCGGTGACTGGGTTAGGGTTAAAACATCGGTTTCAACTCCAACCCACCAGTGGGGAGAGGTGAATCGTACAAGCGTTGGGGTTGTGCATCGGATGGAAAATGAGGAGCTCTGGGTGGCGTTTTGCTTCACAGAGAGGTTGTGGCTTTGCAAGGCATCAGAAATGGAACGGGTTAGGCCGTTTAAAGTGGGGGACAAAGTGAGGATTAGAGAAGGGCTGGTGATCCCACGGTGGGGTTGGGGAATGGAGACACATGCAAGTAAAGGAGAGGTAGTGGGGGTTGACGCAAATGGGAAGTTAAGGATTAAGTTCCGATGGAGAGAAGGGAGGCCATGGATTGGAGATCCTGCTGATATTTCTCTTGATGAAAGCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001101 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005769 GO:0005794 GO:0005802 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006950 GO:0006952 GO:0007034 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009737 GO:0009738 GO:0009755 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010033 GO:0010646 GO:0010648 GO:0012505 GO:0016192 GO:0016197 GO:0016301 GO:0016310 GO:0016567 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0019787 GO:0023051 GO:0023052 GO:0023057 GO:0031410 GO:0031982 GO:0031984 GO:0032446 GO:0032502 GO:0032870 GO:0032940 GO:0033993 GO:0036211 GO:0040007 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043621 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044431 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046903 GO:0046907 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048589 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051716 GO:0065007 GO:0070647 GO:0070887 GO:0071215 GO:0071229 GO:0071310 GO:0071396 GO:0071495 GO:0071704 GO:0097305 GO:0097306 GO:0097708 GO:0098791 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1901701 GO:1905957 GO:1905958
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Pkinase zf-C3HC4 zf-RING_2 zf-RING_5

Protein Analysis

1628

Amino Acids

178.88

Weight (kDa)

5.85

Isoelectric Point (pI)

39.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 5 - 52 6.1e-06 Ring finger domain
zf-RING_UBOX PF13445 6 - 49 3.4e-06 RING-type zinc-finger
PK_Tyr_Ser-Thr PF07714 135 - 426 5.9e-25 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 163 - 426 1.5e-27 Protein kinase domain
Ank_2 PF12796 474 - 575 6.5e-13 Ankyrin repeats (3 copies)
Ank PF00023 512 - 545 6.6e-06 Ankyrin repeat
Ank_4 PF13637 587 - 631 1.9e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 587 - 666 3.9e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 707 - 792 7.2e-14 Ankyrin repeats (3 copies)
Ank_4 PF13637 733 - 782 3.3e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 745 - 806 2.1e-06 Ankyrin repeats (3 copies)
SH3_15 PF18346 869 - 987 1.2e-18 Mind bomb SH3 repeat domain
SH3_15 PF18346 993 - 1113 3.2e-14 Mind bomb SH3 repeat domain
SH3_15 PF18346 1119 - 1163 5.2e-06 Mind bomb SH3 repeat domain
SH3_15 PF18346 1175 - 1237 1e-06 Mind bomb SH3 repeat domain
SH3_15 PF18346 1245 - 1366 2e-16 Mind bomb SH3 repeat domain
SH3_15 PF18346 1372 - 1493 3.8e-18 Mind bomb SH3 repeat domain
SH3_15 PF18346 1502 - 1620 3.5e-14 Mind bomb SH3 repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2364
AarI CACCTGC 2 cut(s) 776, 3873
AatII GACGTC 1 cut(s) 3575
Acc16I TGCGCA 1 cut(s) 1451
Acc36I ACCTGC 2 cut(s) 776, 3873
AccB1I GGYRCC 4 cut(s) 486, 632, 2702, 3535
AccB7I CCANNNNNTGG 6 cut(s) 1104, 1109, 2937, 3596, 4358, 4844
AccBSI CCGCTC 1 cut(s) 1817
AccI GTMKAC 1 cut(s) 1564
AccII CGCG 2 cut(s) 760, 845
AccIII TCCGGA 1 cut(s) 3951
AcoI YGGCCR 8 cut(s) 76, 1537, 1777, 2214, 2310, 2851, 3679, 4315
AcuI CTGAAG 6 cut(s) 53, 899, 1458, 1464, 2571, 3475
AcvI CACGTG 1 cut(s) 2861
AcyI GRCGYC 2 cut(s) 3572, 4011
AdeI CACNNNGTG 1 cut(s) 639
AflIII ACRYGT 2 cut(s) 348, 2908
AhdI GACNNNNNGTC 1 cut(s) 100
AhlI ACTAGT 2 cut(s) 1651, 4019
AjiI CACGTC 3 cut(s) 160, 351, 1568
AjnI CCWGG 7 cut(s) 183, 3049, 3282, 3706, 3768, 4066, 4174
AjuI GAANNNNNNNTTGG 6 cut(s) 890, 922, 2967, 2999, 4254, 4286
AleI CACNNNNGTG 4 cut(s) 1968, 3243, 3524, 4509
AloI GAACNNNNNNTCC 2 cut(s) 2790, 2822
Alw21I GWGCWC 7 cut(s) 213, 337, 396, 605, 1091, 3831, 4617
Alw26I GTCTC 8 cut(s) 166, 247, 524, 1515, 2598, 2610, 3227, 4748
Alw44I GTGCAC 4 cut(s) 392, 601, 1087, 3827
AlwNI CAGNNNCTG 2 cut(s) 190, 3623
Ama87I CYCGRG 3 cut(s) 358, 2535, 4005
Aor13HI TCCGGA 1 cut(s) 3951
ApaLI GTGCAC 4 cut(s) 392, 601, 1087, 3827
Asp700I GAANNNNTTC 6 cut(s) 54, 200, 1398, 1686, 3915, 4234
AspA2I CCTAGG 1 cut(s) 3212
AspLEI GCGC 4 cut(s) 190, 690, 760, 1452
AsuC2I CCSGG 2 cut(s) 497, 2922
AsuII TTCGAA 1 cut(s) 2998
AsuNHI GCTAGC 2 cut(s) 1273, 2143
AvaI CYCGRG 3 cut(s) 358, 2535, 4005
AvaII GGWCC 6 cut(s) 7, 322, 342, 1745, 1819, 2716
AvrII CCTAGG 1 cut(s) 3212
BaeGI GKGCMC 7 cut(s) 396, 605, 1091, 1894, 2707, 3540, 3831
BalI TGGCCA 6 cut(s) 1539, 1779, 2216, 2312, 2853, 4317
BamHI GGATCC 4 cut(s) 927, 1642, 1673, 4465
BanI GGYRCC 4 cut(s) 486, 632, 2702, 3535
BanII GRGCYC 5 cut(s) 1919, 2835, 3282, 3994, 4617
BarI GAAGNNNNNNTAC 4 cut(s) 759, 791, 2071, 2103
BauI CACGAG 3 cut(s) 336, 733, 2751
BbrPI CACGTG 1 cut(s) 2861
Bbv12I GWGCWC 7 cut(s) 213, 337, 396, 605, 1091, 3831, 4617
BbvCI CCTCAGC 1 cut(s) 191
BceAI ACGGC 3 cut(s) 191, 446, 4669
BciT130I CCWGG 7 cut(s) 185, 3051, 3284, 3708, 3770, 4068, 4176
BciVI GTATCC 2 cut(s) 2912, 4199
BcnI CCSGG 2 cut(s) 497, 2922
BcoDI GTCTC 8 cut(s) 166, 247, 524, 1515, 2598, 2610, 3227, 4748
BcuI ACTAGT 2 cut(s) 1651, 4019
BfaI CTAG 9 cut(s) 1200, 1274, 1652, 1725, 1920, 2144, 2487, 3213, 4020
BfmI CTRYAG 3 cut(s) 1041, 1232, 1561
BfoI RGCGCY 1 cut(s) 191
BfuAI ACCTGC 2 cut(s) 776, 3873
BfuI GTATCC 2 cut(s) 2912, 4199
BglI GCCNNNNNGGC 1 cut(s) 2318
BglII AGATCT 3 cut(s) 1462, 1721, 2554
BlnI CCTAGG 1 cut(s) 3212
BlpI GCTNAGC 1 cut(s) 956
Bme1390I CCNGG 9 cut(s) 185, 497, 2922, 3051, 3284, 3708, 3770, 4068, 4176
Bme18I GGWCC 6 cut(s) 7, 322, 342, 1745, 1819, 2716
BmeRI GACNNNNNGTC 1 cut(s) 100
BmeT110I CYCGRG 3 cut(s) 358, 2535, 4005
BmgBI CACGTC 3 cut(s) 160, 351, 1568
BmrFI CCNGG 9 cut(s) 185, 497, 2922, 3051, 3284, 3708, 3770, 4068, 4176
BmrI ACTGGG 4 cut(s) 2629, 3379, 3757, 4525
BmtI GCTAGC 2 cut(s) 1277, 2147
BmuI ACTGGG 4 cut(s) 2629, 3379, 3757, 4525
BoxI GACNNNNGTC 2 cut(s) 461, 1432
BplI GAGNNNNNCTC 2 cut(s) 3976, 4008
Bpu10I CCTNAGC 1 cut(s) 191
Bpu1102I GCTNAGC 1 cut(s) 956
Bpu14I TTCGAA 1 cut(s) 2998
BpuEI CTTGAG 2 cut(s) 907, 2154
BpuMI CCSGG 2 cut(s) 497, 2922
Bsa29I ATCGAT 1 cut(s) 581
BsaAI YACGTR 5 cut(s) 2429, 2513, 2655, 2861, 2909
BsaBI GATNNNNATC 2 cut(s) 1641, 3037
BsaHI GRCGYC 2 cut(s) 3572, 4011
BsaWI WCCGGW 2 cut(s) 3945, 3951
Bse118I RCCGGY 1 cut(s) 373
Bse3DI GCAATG 3 cut(s) 697, 753, 1958
Bse8I GATNNNNATC 2 cut(s) 1641, 3037
BseAI TCCGGA 1 cut(s) 3951
BseBI CCWGG 7 cut(s) 185, 3051, 3284, 3708, 3770, 4068, 4176
BseCI ATCGAT 1 cut(s) 581
BseJI GATNNNNATC 2 cut(s) 1641, 3037
BseMI GCAATG 3 cut(s) 697, 753, 1958
BseMII CTCAG 6 cut(s) 182, 970, 1113, 1764, 3599, 4415
BseRI GAGGAG 8 cut(s) 59, 492, 542, 777, 3158, 4095, 4511, 4625
BseSI GKGCMC 7 cut(s) 396, 605, 1091, 1894, 2707, 3540, 3831
BseYI CCCAGC 1 cut(s) 440
BsgI GTGCAG 2 cut(s) 1596, 4269
Bsh1236I CGCG 2 cut(s) 760, 845
BshNI GGYRCC 4 cut(s) 486, 632, 2702, 3535
BshVI ATCGAT 1 cut(s) 581
BsiHKAI GWGCWC 7 cut(s) 213, 337, 396, 605, 1091, 3831, 4617
BsiHKCI CYCGRG 3 cut(s) 358, 2535, 4005
BsiSI CCGG 6 cut(s) 374, 496, 895, 2922, 3946, 3952
BslFI GGGAC 8 cut(s) 108, 311, 328, 335, 1731, 2811, 3380, 4712
BsmAI GTCTC 8 cut(s) 166, 247, 524, 1515, 2598, 2610, 3227, 4748
BsmBI CGTCTC 3 cut(s) 166, 524, 3227
BsmFI GGGAC 8 cut(s) 108, 311, 328, 335, 1731, 2811, 3380, 4712
BsmI GAATGC 1 cut(s) 1688
BsoBI CYCGRG 3 cut(s) 358, 2535, 4005
Bsp119I TTCGAA 1 cut(s) 2998
Bsp13I TCCGGA 1 cut(s) 3951
Bsp1407I TGTACA 3 cut(s) 1070, 2278, 3835
Bsp1720I GCTNAGC 1 cut(s) 956
Bsp19I CCATGG 5 cut(s) 79, 1103, 3125, 3314, 4838
BspCNI CTCAG 6 cut(s) 183, 969, 1114, 1763, 3598, 4416
BspDI ATCGAT 1 cut(s) 581
BspEI TCCGGA 1 cut(s) 3951
BspFNI CGCG 2 cut(s) 760, 845
BspHI TCATGA 1 cut(s) 2104
BspMAI CTGCAG 2 cut(s) 1045, 1236
BspMI ACCTGC 2 cut(s) 776, 3873
BspOI GCTAGC 2 cut(s) 1277, 2147
BspT104I TTCGAA 1 cut(s) 2998
BspT107I GGYRCC 4 cut(s) 486, 632, 2702, 3535
BsrBI CCGCTC 1 cut(s) 1817
BsrDI GCAATG 3 cut(s) 697, 753, 1958
BsrFI RCCGGY 1 cut(s) 373
BsrGI TGTACA 3 cut(s) 1070, 2278, 3835
BssAI RCCGGY 1 cut(s) 373
BssNI GRCGYC 2 cut(s) 3572, 4011
BssSI CACGAG 3 cut(s) 336, 733, 2751
BssT1I CCWWGG 7 cut(s) 79, 1103, 2787, 3125, 3212, 3314, 4838
Bst2BI CACGAG 3 cut(s) 336, 733, 2751
Bst2UI CCWGG 7 cut(s) 185, 3051, 3284, 3708, 3770, 4068, 4176
Bst4CI ACNGT 9 cut(s) 813, 1499, 2498, 2878, 3434, 3625, 3833, 4338, 4737
Bst6I CTCTTC 6 cut(s) 121, 1422, 2022, 2116, 2301, 4488
BstACI GRCGYC 2 cut(s) 3572, 4011
BstAPI GCANNNNNTGC 3 cut(s) 96, 2140, 2411
BstAUI TGTACA 3 cut(s) 1070, 2278, 3835
BstBAI YACGTR 5 cut(s) 2429, 2513, 2655, 2861, 2909
BstBI TTCGAA 1 cut(s) 2998
BstDEI CTNAG 8 cut(s) 191, 722, 956, 1122, 1750, 2459, 3585, 4424
BstDSI CCRYGG 6 cut(s) 79, 1103, 3125, 3314, 4733, 4838
BstEII GGTNACC 1 cut(s) 1406
BstENI CCTNNNNNAGG 1 cut(s) 2791
BstFNI CGCG 2 cut(s) 760, 845
BstH2I RGCGCY 1 cut(s) 191
BstHHI GCGC 4 cut(s) 190, 690, 760, 1452
BstMAI GTCTC 8 cut(s) 166, 247, 524, 1515, 2598, 2610, 3227, 4748
BstNI CCWGG 7 cut(s) 185, 3051, 3284, 3708, 3770, 4068, 4176
BstNSI RCATGY 2 cut(s) 2807, 4763
BstPAI GACNNNNGTC 2 cut(s) 461, 1432
BstPI GGTNACC 1 cut(s) 1406
BstSCI CCNGG 9 cut(s) 183, 495, 2920, 3049, 3282, 3706, 3768, 4066, 4174
BstSFI CTRYAG 3 cut(s) 1041, 1232, 1561
BstSLI GKGCMC 7 cut(s) 396, 605, 1091, 1894, 2707, 3540, 3831
BstUI CGCG 2 cut(s) 760, 845
BstXI CCANNNNNNTGG 5 cut(s) 984, 3126, 3248, 3315, 4074
Bsu15I ATCGAT 1 cut(s) 581
BsuI GTATCC 2 cut(s) 2912, 4199
BsuTUI ATCGAT 1 cut(s) 581
BtgI CCRYGG 6 cut(s) 79, 1103, 3125, 3314, 4733, 4838
BtrI CACGTC 3 cut(s) 160, 351, 1568
BtsI GCAGTG 4 cut(s) 1459, 1579, 2067, 4186
BveI ACCTGC 2 cut(s) 776, 3873
CaiI CAGNNNCTG 2 cut(s) 190, 3623
CciI TCATGA 1 cut(s) 2104
CfoI GCGC 4 cut(s) 190, 690, 760, 1452
Cfr10I RCCGGY 1 cut(s) 373
ClaI ATCGAT 1 cut(s) 581
CseI GACGC 3 cut(s) 3244, 4000, 4799
CsiI ACCWGGT 1 cut(s) 3049
CspCI CAANNNNNGTGG 2 cut(s) 4222, 4257
DdeI CTNAG 8 cut(s) 191, 722, 956, 1122, 1750, 2459, 3585, 4424
DraI TTTAAA 3 cut(s) 2632, 3928, 4690
DraIII CACNNNGTG 1 cut(s) 639
DriI GACNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 8 cut(s) 76, 1537, 1777, 2214, 2310, 2851, 3679, 4315
Eam1104I CTCTTC 6 cut(s) 121, 1422, 2022, 2116, 2301, 4488
Eam1105I GACNNNNNGTC 1 cut(s) 100
EarI CTCTTC 6 cut(s) 121, 1422, 2022, 2116, 2301, 4488
EciI GGCGGA 1 cut(s) 433
Ecl136II GAGCTC 1 cut(s) 4615
Eco130I CCWWGG 7 cut(s) 79, 1103, 2787, 3125, 3212, 3314, 4838
Eco24I GRGCYC 5 cut(s) 1919, 2835, 3282, 3994, 4617
Eco32I GATATC 2 cut(s) 2547, 3421
Eco47I GGWCC 6 cut(s) 7, 322, 342, 1745, 1819, 2716
Eco53kI GAGCTC 1 cut(s) 4615
Eco57I CTGAAG 6 cut(s) 53, 899, 1458, 1464, 2571, 3475
Eco72I CACGTG 1 cut(s) 2861
Eco88I CYCGRG 3 cut(s) 358, 2535, 4005
Eco91I GGTNACC 1 cut(s) 1406
EcoICRI GAGCTC 1 cut(s) 4615
EcoNI CCTNNNNNAGG 1 cut(s) 2791
EcoO109I RGGNCCY 3 cut(s) 7, 342, 2527
EcoO65I GGTNACC 1 cut(s) 1406
EcoRI GAATTC 4 cut(s) 1194, 1939, 2975, 3000
EcoRII CCWGG 7 cut(s) 183, 3049, 3282, 3706, 3768, 4066, 4174
EcoRV GATATC 2 cut(s) 2547, 3421
EcoT14I CCWWGG 7 cut(s) 79, 1103, 2787, 3125, 3212, 3314, 4838
EcoT22I ATGCAT 2 cut(s) 1057, 3223
EcoT38I GRGCYC 5 cut(s) 1919, 2835, 3282, 3994, 4617
ErhI CCWWGG 7 cut(s) 79, 1103, 2787, 3125, 3212, 3314, 4838
Esp3I CGTCTC 3 cut(s) 166, 524, 3227
FalI AAGNNNNNCTT 8 cut(s) 83, 115, 1243, 1275, 4467, 4499, 4485, 4517
FaqI GGGAC 8 cut(s) 108, 311, 328, 335, 1731, 2811, 3380, 4712
FauI CCCGC 5 cut(s) 41, 155, 356, 362, 567
FblI GTMKAC 1 cut(s) 1564
FriOI GRGCYC 5 cut(s) 1919, 2835, 3282, 3994, 4617
FspBI CTAG 9 cut(s) 1200, 1274, 1652, 1725, 1920, 2144, 2487, 3213, 4020
FspI TGCGCA 1 cut(s) 1451
GlaI GCGC 4 cut(s) 189, 689, 759, 1451
GsaI CCCAGC 1 cut(s) 444
HaeII RGCGCY 1 cut(s) 191
HapII CCGG 6 cut(s) 374, 496, 895, 2922, 3946, 3952
HgaI GACGC 3 cut(s) 3244, 4000, 4799
HhaI GCGC 4 cut(s) 190, 690, 760, 1452
Hin1I GRCGYC 2 cut(s) 3572, 4011
Hin6I GCGC 4 cut(s) 188, 688, 758, 1450
HinP1I GCGC 4 cut(s) 188, 688, 758, 1450
HincII GTYRAC 1 cut(s) 4789
HindII GTYRAC 1 cut(s) 4789
HindIII AAGCTT 2 cut(s) 3384, 4452
HpaII CCGG 6 cut(s) 374, 496, 895, 2922, 3946, 3952
Hpy99I CGWCG 5 cut(s) 44, 170, 173, 290, 355
HpyCH4III ACNGT 9 cut(s) 813, 1499, 2498, 2878, 3434, 3625, 3833, 4338, 4737
HpyF3I CTNAG 8 cut(s) 191, 722, 956, 1122, 1750, 2459, 3585, 4424
Hsp92I GRCGYC 2 cut(s) 3572, 4011
HspAI GCGC 4 cut(s) 188, 688, 758, 1450
KflI GGGWCCC 1 cut(s) 342
Kpn2I TCCGGA 1 cut(s) 3951
MabI ACCWGGT 1 cut(s) 3049
MaeI CTAG 9 cut(s) 1200, 1274, 1652, 1725, 1920, 2144, 2487, 3213, 4020
MbiI CCGCTC 1 cut(s) 1817
MfeI CAATTG 2 cut(s) 2043, 4404
MlsI TGGCCA 6 cut(s) 1539, 1779, 2216, 2312, 2853, 4317
MluNI TGGCCA 6 cut(s) 1539, 1779, 2216, 2312, 2853, 4317
MlyI GAGTC 4 cut(s) 1055, 1144, 3387, 3717
MmeI TCCRAC 9 cut(s) 1171, 2186, 2314, 3229, 3607, 4102, 4165, 4222, 4571
Mox20I TGGCCA 6 cut(s) 1539, 1779, 2216, 2312, 2853, 4317
Mph1103I ATGCAT 2 cut(s) 1057, 3223
MroI TCCGGA 1 cut(s) 3951
MroXI GAANNNNTTC 6 cut(s) 54, 200, 1398, 1686, 3915, 4234
MscI TGGCCA 6 cut(s) 1539, 1779, 2216, 2312, 2853, 4317
MslI CAYNNNNRTG 9 cut(s) 583, 1764, 1968, 2682, 3243, 3524, 4032, 4377, 4509
Msp20I TGGCCA 6 cut(s) 1539, 1779, 2216, 2312, 2853, 4317
MspA1I CMGCKG 5 cut(s) 150, 257, 559, 2812, 4442
MspI CCGG 6 cut(s) 374, 496, 895, 2922, 3946, 3952
MspR9I CCNGG 9 cut(s) 185, 497, 2922, 3051, 3284, 3708, 3770, 4068, 4176
MunI CAATTG 2 cut(s) 2043, 4404
Mva1269I GAATGC 1 cut(s) 1688
MvaI CCWGG 7 cut(s) 185, 3051, 3284, 3708, 3770, 4068, 4176
MvnI CGCG 2 cut(s) 760, 845
NciI CCSGG 2 cut(s) 497, 2922
NcoI CCATGG 5 cut(s) 79, 1103, 3125, 3314, 4838
NheI GCTAGC 2 cut(s) 1273, 2143
NsbI TGCGCA 1 cut(s) 1451
NsiI ATGCAT 2 cut(s) 1057, 3223
NspI RCATGY 2 cut(s) 2807, 4763
NspV TTCGAA 1 cut(s) 2998
OliI CACNNNNGTG 4 cut(s) 1968, 3243, 3524, 4509
PaeR7I CTCGAG 1 cut(s) 4005
PagI TCATGA 1 cut(s) 2104
PaqCI CACCTGC 2 cut(s) 776, 3873
PcsI WCGNNNNNNNCGW 2 cut(s) 165, 177
PctI GAATGC 1 cut(s) 1688
PdmI GAANNNNTTC 6 cut(s) 54, 200, 1398, 1686, 3915, 4234
PflFI GACNNNGTC 1 cut(s) 2621
PflMI CCANNNNNTGG 6 cut(s) 1104, 1109, 2937, 3596, 4358, 4844
PfoI TCCNGGA 2 cut(s) 2920, 3706
PleI GAGTC 4 cut(s) 1054, 1144, 3386, 3717
PmaCI CACGTG 1 cut(s) 2861
PmlI CACGTG 1 cut(s) 2861
PpsI GAGTC 4 cut(s) 1054, 1144, 3386, 3717
Ppu21I YACGTR 5 cut(s) 2429, 2513, 2655, 2861, 2909
PpuMI RGGWCCY 2 cut(s) 7, 342
PshAI GACNNNNGTC 2 cut(s) 461, 1432
PsiI TTATAA 1 cut(s) 2364
Psp124BI GAGCTC 1 cut(s) 4617
Psp5II RGGWCCY 2 cut(s) 7, 342
Psp6I CCWGG 7 cut(s) 183, 3049, 3282, 3706, 3768, 4066, 4174
PspCI CACGTG 1 cut(s) 2861
PspEI GGTNACC 1 cut(s) 1406
PspFI CCCAGC 1 cut(s) 440
PspGI CCWGG 7 cut(s) 183, 3049, 3282, 3706, 3768, 4066, 4174
PspPPI RGGWCCY 2 cut(s) 7, 342
PsrI GAACNNNNNNTAC 2 cut(s) 3932, 3964
PstI CTGCAG 2 cut(s) 1045, 1236
PstNI CAGNNNCTG 2 cut(s) 190, 3623
PsyI GACNNNGTC 1 cut(s) 2621
PvuII CAGCTG 3 cut(s) 559, 2812, 4442
RseI CAYNNNNRTG 9 cut(s) 583, 1764, 1968, 2682, 3243, 3524, 4032, 4377, 4509
SacI GAGCTC 1 cut(s) 4617
SchI GAGTC 4 cut(s) 1055, 1144, 3387, 3717
ScrFI CCNGG 9 cut(s) 185, 497, 2922, 3051, 3284, 3708, 3770, 4068, 4176
SexAI ACCWGGT 1 cut(s) 3049
SfcI CTRYAG 3 cut(s) 1041, 1232, 1561
Sfr274I CTCGAG 1 cut(s) 4005
SfuI TTCGAA 1 cut(s) 2998
SinI GGWCC 6 cut(s) 7, 322, 342, 1745, 1819, 2716
SlaI CTCGAG 1 cut(s) 4005
SmiMI CAYNNNNRTG 9 cut(s) 583, 1764, 1968, 2682, 3243, 3524, 4032, 4377, 4509
SmlI CTYRAG 3 cut(s) 922, 2169, 4005
SmoI CTYRAG 3 cut(s) 922, 2169, 4005
SpeI ACTAGT 2 cut(s) 1651, 4019
SspI AATATT 1 cut(s) 1506
SspMI CTAG 9 cut(s) 1200, 1274, 1652, 1725, 1920, 2144, 2487, 3213, 4020
SstI GAGCTC 1 cut(s) 4617
StyD4I CCNGG 9 cut(s) 183, 495, 2920, 3049, 3282, 3706, 3768, 4066, 4174
StyI CCWWGG 7 cut(s) 79, 1103, 2787, 3125, 3212, 3314, 4838
TaaI ACNGT 9 cut(s) 813, 1499, 2498, 2878, 3434, 3625, 3833, 4338, 4737
TaqII GACCGA 1 cut(s) 339
TatI WGTACW 5 cut(s) 1070, 1499, 1852, 2278, 3835
TauI GCSGC 5 cut(s) 78, 257, 418, 492, 574
Tth111I GACNNNGTC 1 cut(s) 2621
Van91I CCANNNNNTGG 6 cut(s) 1104, 1109, 2937, 3596, 4358, 4844
VneI GTGCAC 4 cut(s) 392, 601, 1087, 3827
VpaK11BI GGWCC 6 cut(s) 7, 322, 342, 1745, 1819, 2716
XagI CCTNNNNNAGG 1 cut(s) 2791
XceI RCATGY 2 cut(s) 2807, 4763
XcmI CCANNNNNNNNNTGG 8 cut(s) 823, 3597, 3789, 3978, 4359, 4385, 4554, 4740
XhoI CTCGAG 1 cut(s) 4005
XmaJI CCTAGG 1 cut(s) 3212
XmiI GTMKAC 1 cut(s) 1564
XmnI GAANNNNTTC 6 cut(s) 54, 200, 1398, 1686, 3915, 4234
XspI CTAG 9 cut(s) 1200, 1274, 1652, 1725, 1920, 2144, 2487, 3213, 4020
ZraI GACGTC 1 cut(s) 3573
Zsp2I ATGCAT 2 cut(s) 1057, 3223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.