AT5G14030

TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009086
Physical Location & Seq
Forward (+)
4526766 .. 4529614
2849 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14030.1

Sequence Viewer

Length: 588 bp
ATGGCCGTCGCTGTAGCTAAGCTTCTGATCTCCGCCATGGCGGTTTTCATGCTTGTTTCTGCTTCGTTCGCGACCTCGGAAGTGCCGTTCATGGTGGTTCACAAGAAAGCCACTCTCAACAGGCTCAAATCTGGCGCCGAACGCGTCTCCGTTTCCTACGACATCTATAACCAAGGATCCTCGTCGGCGTATGATGTGACTCTGACAGATAATAGCTGGGATAAAAAGACTTTTGAAGTTGTTAATGGAAACACTTCAAAATCATGGGAAAGACTTGATGCAGGAGGTATTCTGTCTCATTCTATCGAATTGGAGGCCAAGGTTAAAGGAGTCTTCTACGGTGCTCCTGCTGTCGTTACTTTCCGCATCCCCACTAAGCCAGCTCTTCAGGAAGCATACTCAACTCCACTACTACCTCTAGATATCCTCGCAGACAAACCTCCAACGAAACCTTTGGACGTGGCCAAGAGGTTGCTGGCGAAATATGGATCACTCGTCTCCGTGATCTCCATGGTGGTTTGTTTCATATACTTGGTGGCAACACCTAAGTCCAACGTATCAAAGGCAAGCAGCAAGAAGAAGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
TRAP_beta

Protein Analysis

195

Amino Acids

21.1

Weight (kDa)

9.66

Isoelectric Point (pI)

29.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAP_beta PF05753 13 - 188 5.1e-59 Translocon-associated protein beta (TRAPB)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 134
AccII CGCG 2 cut(s) 71, 144
AciI CCGC 3 cut(s) 33, 41, 364
AclWI GGATC 3 cut(s) 171, 184, 496
AcoI YGGCCR 2 cut(s) 3, 462
AcuI CTGAAG 1 cut(s) 371
AcyI GRCGYC 1 cut(s) 135
AflIII ACRYGT 1 cut(s) 142
AgsI TTSAA 2 cut(s) 236, 258
AjiI CACGTC 1 cut(s) 460
AloI GAACNNNNNNTCC 2 cut(s) 71, 103
AluBI AGCT 4 cut(s) 17, 22, 216, 383
AluI AGCT 4 cut(s) 17, 22, 216, 383
Alw21I GWGCWC 1 cut(s) 346
Alw26I GTCTC 3 cut(s) 151, 300, 502
AlwI GGATC 3 cut(s) 171, 184, 496
AoxI GGCC 3 cut(s) 3, 315, 462
ApeKI GCWGC 1 cut(s) 570
Asp700I GAANNNNTTC 1 cut(s) 253
AspLEI GCGC 1 cut(s) 137
BalI TGGCCA 1 cut(s) 464
BamHI GGATCC 1 cut(s) 176
BanI GGYRCC 1 cut(s) 134
BarI GAAGNNNNNNTAC 1 cut(s) 38
BbsI GAAGAC 1 cut(s) 325
Bbv12I GWGCWC 1 cut(s) 346
BbvI GCAGC 1 cut(s) 582
BceAI ACGGC 1 cut(s) 70
BcoDI GTCTC 3 cut(s) 151, 300, 502
BfaI CTAG 1 cut(s) 419
BfmI CTRYAG 1 cut(s) 12
BfoI RGCGCY 1 cut(s) 138
BisI GCNGC 1 cut(s) 571
BlpI GCTNAGC 1 cut(s) 18
BlsI GCNGC 1 cut(s) 572
BmgBI CACGTC 1 cut(s) 460
BmiI GGNNCC 2 cut(s) 136, 178
BmsI GCATC 2 cut(s) 268, 375
BpiI GAAGAC 1 cut(s) 325
Bpu1102I GCTNAGC 1 cut(s) 18
BsaHI GRCGYC 1 cut(s) 135
BsaJI CCNNGG 5 cut(s) 36, 75, 172, 318, 510
BseDI CCNNGG 5 cut(s) 36, 75, 172, 318, 510
BseGI GGATG 1 cut(s) 366
BseXI GCAGC 1 cut(s) 582
BseYI CCCAGC 1 cut(s) 216
Bsh1236I CGCG 2 cut(s) 71, 144
BshFI GGCC 3 cut(s) 5, 317, 464
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 1 cut(s) 346
BsmAI GTCTC 3 cut(s) 151, 300, 502
BsmBI CGTCTC 2 cut(s) 151, 502
BsnI GGCC 3 cut(s) 5, 317, 464
Bsp1286I GDGCHC 1 cut(s) 346
Bsp143I GATC 4 cut(s) 27, 176, 488, 504
Bsp1720I GCTNAGC 1 cut(s) 18
Bsp19I CCATGG 2 cut(s) 36, 510
Bsp68I TCGCGA 1 cut(s) 71
BspACI CCGC 3 cut(s) 33, 41, 364
BspANI GGCC 3 cut(s) 5, 317, 464
BspFNI CGCG 2 cut(s) 71, 144
BspLI GGNNCC 2 cut(s) 136, 178
BspPI GGATC 3 cut(s) 171, 184, 496
BspQI GCTCTTC 1 cut(s) 390
BspT107I GGYRCC 1 cut(s) 134
BssECI CCNNGG 5 cut(s) 36, 75, 172, 318, 510
BssMI GATC 4 cut(s) 27, 176, 488, 504
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 4 cut(s) 36, 172, 318, 510
Bst4CI ACNGT 1 cut(s) 341
Bst6I CTCTTC 1 cut(s) 390
BstACI GRCGYC 1 cut(s) 135
BstC8I GCNNGC 3 cut(s) 381, 477, 568
BstDEI CTNAG 3 cut(s) 18, 375, 546
BstDSI CCRYGG 2 cut(s) 36, 510
BstF5I GGATG 1 cut(s) 366
BstFNI CGCG 2 cut(s) 71, 144
BstH2I RGCGCY 1 cut(s) 138
BstHHI GCGC 1 cut(s) 137
BstKTI GATC 4 cut(s) 30, 179, 491, 507
BstMAI GTCTC 3 cut(s) 151, 300, 502
BstMBI GATC 4 cut(s) 27, 176, 488, 504
BstMWI GCNNNNNNNGC 2 cut(s) 68, 141
BstSFI CTRYAG 1 cut(s) 12
BstUI CGCG 2 cut(s) 71, 144
BstV1I GCAGC 1 cut(s) 582
BstV2I GAAGAC 1 cut(s) 325
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BsuRI GGCC 3 cut(s) 5, 317, 464
BtgI CCRYGG 2 cut(s) 36, 510
BtrI CACGTC 1 cut(s) 460
BtsCI GGATG 1 cut(s) 366
BtuMI TCGCGA 1 cut(s) 71
Cac8I GCNNGC 3 cut(s) 381, 477, 568
CfoI GCGC 1 cut(s) 137
CseI GACGC 1 cut(s) 133
CviAII CATG 5 cut(s) 37, 49, 91, 264, 511
DdeI CTNAG 3 cut(s) 18, 375, 546
DinI GGCGCC 1 cut(s) 136
DpnI GATC 4 cut(s) 29, 178, 490, 506
DpnII GATC 4 cut(s) 27, 176, 488, 504
EaeI YGGCCR 2 cut(s) 3, 462
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
EciI GGCGGA 1 cut(s) 22
Eco130I CCWWGG 4 cut(s) 36, 172, 318, 510
Eco32I GATATC 1 cut(s) 424
Eco57I CTGAAG 1 cut(s) 371
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 4 cut(s) 36, 172, 318, 510
EgeI GGCGCC 1 cut(s) 136
EheI GGCGCC 1 cut(s) 136
ErhI CCWWGG 4 cut(s) 36, 172, 318, 510
Esp3I CGTCTC 2 cut(s) 151, 502
FaeI CATG 5 cut(s) 40, 52, 94, 267, 514
FatI CATG 5 cut(s) 36, 48, 90, 263, 510
Fnu4HI GCNGC 1 cut(s) 571
FokI GGATG 1 cut(s) 353
Fsp4HI GCNGC 1 cut(s) 571
FspBI CTAG 1 cut(s) 419
GlaI GCGC 1 cut(s) 136
GluI GCNGC 1 cut(s) 571
GsaI CCCAGC 1 cut(s) 220
HaeII RGCGCY 1 cut(s) 138
HaeIII GGCC 3 cut(s) 5, 317, 464
HgaI GACGC 1 cut(s) 133
HhaI GCGC 1 cut(s) 137
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 5 cut(s) 40, 52, 94, 267, 514
Hin6I GCGC 1 cut(s) 135
HinP1I GCGC 1 cut(s) 135
HindIII AAGCTT 1 cut(s) 20
HinfI GANTC 2 cut(s) 199, 330
Hpy166II GTNNAC 1 cut(s) 100
Hpy188I TCNGA 3 cut(s) 27, 79, 204
Hpy188III TCNNGA 3 cut(s) 70, 389, 419
Hpy8I GTNNAC 1 cut(s) 100
Hpy99I CGWCG 2 cut(s) 11, 187
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 2 cut(s) 459, 555
HpyCH4V TGCA 1 cut(s) 281
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 141
HpyF3I CTNAG 3 cut(s) 18, 375, 546
HpySE526I ACGT 2 cut(s) 459, 555
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 5 cut(s) 40, 52, 94, 267, 514
HspAI GCGC 1 cut(s) 135
KasI GGCGCC 1 cut(s) 134
Kzo9I GATC 4 cut(s) 27, 176, 488, 504
LguI GCTCTTC 1 cut(s) 390
LmnI GCTCC 1 cut(s) 349
LpnPI CCDG 8 cut(s) 106, 117, 202, 267, 360, 374, 393, 461
Lsp1109I GCAGC 1 cut(s) 582
LweI GCATC 2 cut(s) 268, 375
MaeI CTAG 1 cut(s) 419
MaeII ACGT 2 cut(s) 459, 555
MaeIII GTNAC 2 cut(s) 196, 355
MalI GATC 4 cut(s) 29, 178, 490, 506
MboI GATC 4 cut(s) 27, 176, 488, 504
MboII GAAGA 2 cut(s) 325, 377
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 1 cut(s) 346
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 1 cut(s) 308
MluI ACGCGT 1 cut(s) 142
MluNI TGGCCA 1 cut(s) 464
Mly113I GGCGCC 1 cut(s) 135
MlyI GAGTC 2 cut(s) 193, 339
MmeI TCCRAC 2 cut(s) 467, 576
MnlI CCTC 8 cut(s) 85, 190, 278, 307, 426, 437, 450, 462
Mox20I TGGCCA 1 cut(s) 464
MroXI GAANNNNTTC 1 cut(s) 253
MscI TGGCCA 1 cut(s) 464
MseI TTAA 3 cut(s) 243, 324, 586
Msp20I TGGCCA 1 cut(s) 464
MvnI CGCG 2 cut(s) 71, 144
MwoI GCNNNNNNNGC 2 cut(s) 68, 141
NarI GGCGCC 1 cut(s) 135
NcoI CCATGG 2 cut(s) 36, 510
NdeII GATC 4 cut(s) 27, 176, 488, 504
NlaIII CATG 5 cut(s) 40, 52, 94, 267, 514
NlaIV GGNNCC 2 cut(s) 136, 178
NmuCI GTSAC 1 cut(s) 196
NruI TCGCGA 1 cut(s) 71
PciSI GCTCTTC 1 cut(s) 390
PcsI WCGNNNNNNNCGW 1 cut(s) 83
PdmI GAANNNNTTC 1 cut(s) 253
PkrI GCNGC 1 cut(s) 572
PleI GAGTC 2 cut(s) 193, 338
PluTI GGCGCC 1 cut(s) 138
PpsI GAGTC 2 cut(s) 193, 338
PspFI CCCAGC 1 cut(s) 216
PspN4I GGNNCC 2 cut(s) 136, 178
PsuI RGATCY 1 cut(s) 176
RruI TCGCGA 1 cut(s) 71
SapI GCTCTTC 1 cut(s) 390
SaqAI TTAA 3 cut(s) 243, 324, 586
SatI GCNGC 1 cut(s) 571
Sau3AI GATC 4 cut(s) 27, 176, 488, 504
SchI GAGTC 2 cut(s) 193, 339
SduI GDGCHC 1 cut(s) 346
SfaNI GCATC 2 cut(s) 268, 375
SfcI CTRYAG 1 cut(s) 12
SfoI GGCGCC 1 cut(s) 136
Sse9I AATT 1 cut(s) 308
SsiI CCGC 3 cut(s) 33, 41, 364
SspDI GGCGCC 1 cut(s) 134
SspMI CTAG 1 cut(s) 419
StyI CCWWGG 4 cut(s) 36, 172, 318, 510
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 2 cut(s) 462, 558
TaqI TCGA 1 cut(s) 306
TasI AATT 1 cut(s) 308
Tru1I TTAA 3 cut(s) 243, 324, 586
Tru9I TTAA 3 cut(s) 243, 324, 586
TseFI GTSAC 1 cut(s) 196
TseI GCWGC 1 cut(s) 570
Tsp45I GTSAC 1 cut(s) 196
TspDTI ATGAA 3 cut(s) 37, 79, 514
TspGWI ACGGA 2 cut(s) 139, 490
XbaI TCTAGA 1 cut(s) 418
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XmnI GAANNNNTTC 1 cut(s) 253
XspI CTAG 1 cut(s) 419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.