MD09G1005300.v1.1

Translocon-associated protein subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009086
Physical Location & Seq
Reverse (-)
404143 .. 406757
2615 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1005300.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGCGATTCCGATCACCAACGCTCTCCTCTGGACGGCGGTAGCTCTTTTTCTGTTCACGTCAGCGCTCGCGAGCTCCGACGTGCCTTTCATCGTCACCCACAAGAAGGCCTCCCTCAACCGGCTCAAGTCCGGCGCCGAGCGCGTCCTTGTCTCCATCGACATCTACAACCAAGGATCTTCCACGGCTTATGATGTAAGCTTGAGTGATGAGACCTGGCCTCAGGATATCTTCGATGTTGTTAGTGGTAGCACTTCCAAGTCATGGGAAAGGCTTGATGCAGGTGGCATTATATCCCACTCTTTTGAATTGGAGGGCAAAGAAAAATTACTTTTCAACGGTGCACCAGCTCTTATTACATTCCGCATTCCCACAAAGGCTGCTTTACAGGAGTCTTATTCAACTCCAATATTTCCTTTAGATGTTCTTGCAGACAGGCCTCCTGAGAAGAAATTTGAGTGGGTTAAGAGGCTTCTGGCAAAGTATGGGTCTCTGATCTCTGTGGTGTCCATTGTGGTTCTATTTGTGTACCTGGTTGCCACTCCATCCAAATCCGGTGCTGCAAAAGGAAGCAAGAAGAAGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
TRAP_beta

Protein Analysis

196

Amino Acids

21.28

Weight (kDa)

9.37

Isoelectric Point (pI)

24.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAP_beta PF05753 16 - 191 1.3e-54 Translocon-associated protein beta (TRAPB)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 272
Acc36I ACCTGC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 134
AccB7I CCANNNNNTGG 1 cut(s) 264
AccII CGCG 2 cut(s) 71, 144
AciI CCGC 2 cut(s) 38, 364
AclWI GGATC 1 cut(s) 184
AcsI RAATTY 1 cut(s) 452
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 1 cut(s) 530
AfeI AGCGCT 1 cut(s) 66
AfiI CCNNNNNNNGG 4 cut(s) 34, 106, 120, 264
AgsI TTSAA 3 cut(s) 308, 337, 402
AjiI CACGTC 2 cut(s) 60, 82
AjnI CCWGG 2 cut(s) 215, 531
AluBI AGCT 4 cut(s) 44, 75, 201, 350
AluI AGCT 4 cut(s) 44, 75, 201, 350
Alw21I GWGCWC 2 cut(s) 77, 346
Alw26I GTCTC 3 cut(s) 157, 206, 495
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 1 cut(s) 184
Aor51HI AGCGCT 1 cut(s) 66
AoxI GGCC 3 cut(s) 108, 218, 437
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 2 cut(s) 380, 560
ApoI RAATTY 1 cut(s) 452
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
AspLEI GCGC 3 cut(s) 67, 137, 144
AsuHPI GGTGA 2 cut(s) 7, 88
AxyI CCTNAGG 1 cut(s) 222
BaeGI GKGCMC 1 cut(s) 346
BanI GGYRCC 1 cut(s) 134
BanII GRGCYC 1 cut(s) 77
Bbv12I GWGCWC 2 cut(s) 77, 346
BbvI GCAGC 2 cut(s) 367, 547
BccI CCATC 2 cut(s) 164, 553
BceAI ACGGC 2 cut(s) 51, 201
BciT130I CCWGG 2 cut(s) 217, 533
BcoDI GTCTC 3 cut(s) 157, 206, 495
BfoI RGCGCY 2 cut(s) 68, 138
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 2 cut(s) 381, 561
BlsI GCNGC 2 cut(s) 382, 562
Bme1390I CCNGG 2 cut(s) 217, 533
BmgBI CACGTC 2 cut(s) 60, 82
BmiI GGNNCC 1 cut(s) 136
BmrFI CCNGG 2 cut(s) 217, 533
BmsI GCATC 1 cut(s) 268
BpuEI CTTGAG 2 cut(s) 110, 223
BsaBI GATNNNNATC 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 135
BsaI GGTCTC 2 cut(s) 206, 495
BsaJI CCNNGG 2 cut(s) 172, 183
BsaWI WCCGGW 1 cut(s) 554
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 106, 120, 264
Bse118I RCCGGY 1 cut(s) 120
Bse21I CCTNAGG 1 cut(s) 222
Bse8I GATNNNNATC 1 cut(s) 11
BseBI CCWGG 2 cut(s) 217, 533
BseDI CCNNGG 2 cut(s) 172, 183
BseGI GGATG 1 cut(s) 545
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 4 cut(s) 34, 106, 120, 264
BseMII CTCAG 2 cut(s) 236, 435
BseRI GAGGAG 1 cut(s) 17
BseSI GKGCMC 1 cut(s) 346
BseXI GCAGC 2 cut(s) 367, 547
Bsh1236I CGCG 2 cut(s) 71, 144
BshFI GGCC 3 cut(s) 110, 220, 439
BshNI GGYRCC 1 cut(s) 134
BsiHKAI GWGCWC 2 cut(s) 77, 346
BsiSI CCGG 3 cut(s) 121, 132, 555
BslI CCNNNNNNNGG 4 cut(s) 34, 106, 120, 264
BsmAI GTCTC 3 cut(s) 157, 206, 495
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 3 cut(s) 110, 220, 439
Bso31I GGTCTC 2 cut(s) 206, 495
Bsp1286I GDGCHC 2 cut(s) 77, 346
Bsp143I GATC 3 cut(s) 12, 176, 495
Bsp68I TCGCGA 1 cut(s) 71
BspACI CCGC 2 cut(s) 38, 364
BspANI GGCC 3 cut(s) 110, 220, 439
BspCNI CTCAG 2 cut(s) 235, 436
BspFNI CGCG 2 cut(s) 71, 144
BspLI GGNNCC 1 cut(s) 136
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 1 cut(s) 184
BspT107I GGYRCC 1 cut(s) 134
BspTNI GGTCTC 2 cut(s) 206, 495
BsrFI RCCGGY 1 cut(s) 120
BssAI RCCGGY 1 cut(s) 120
BssECI CCNNGG 2 cut(s) 172, 183
BssMI GATC 3 cut(s) 12, 176, 495
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 172
Bst2UI CCWGG 2 cut(s) 217, 533
Bst4CI ACNGT 1 cut(s) 341
BstACI GRCGYC 1 cut(s) 135
BstC8I GCNNGC 2 cut(s) 69, 73
BstDEI CTNAG 2 cut(s) 222, 444
BstDSI CCRYGG 1 cut(s) 183
BstF5I GGATG 1 cut(s) 545
BstFNI CGCG 2 cut(s) 71, 144
BstH2I RGCGCY 2 cut(s) 68, 138
BstHHI GCGC 3 cut(s) 67, 137, 144
BstKTI GATC 3 cut(s) 15, 179, 498
BstMAI GTCTC 3 cut(s) 157, 206, 495
BstMBI GATC 3 cut(s) 12, 176, 495
BstMWI GCNNNNNNNGC 1 cut(s) 141
BstNI CCWGG 2 cut(s) 217, 533
BstSCI CCNGG 2 cut(s) 215, 531
BstSLI GKGCMC 1 cut(s) 346
BstUI CGCG 2 cut(s) 71, 144
BstV1I GCAGC 2 cut(s) 367, 547
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
Bsu36I CCTNAGG 1 cut(s) 222
BsuRI GGCC 3 cut(s) 110, 220, 439
BtgI CCRYGG 1 cut(s) 183
BtrI CACGTC 2 cut(s) 60, 82
BtsCI GGATG 1 cut(s) 545
BtuMI TCGCGA 1 cut(s) 71
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 2 cut(s) 69, 73
CfoI GCGC 3 cut(s) 67, 137, 144
Cfr10I RCCGGY 1 cut(s) 120
CseI GACGC 1 cut(s) 133
CsiI ACCWGGT 1 cut(s) 531
Csp6I GTAC 1 cut(s) 529
CviAII CATG 1 cut(s) 264
CviQI GTAC 1 cut(s) 529
DdeI CTNAG 2 cut(s) 222, 444
DinI GGCGCC 1 cut(s) 136
DpnI GATC 3 cut(s) 14, 178, 497
DpnII GATC 3 cut(s) 12, 176, 495
Ecl136II GAGCTC 1 cut(s) 75
Eco130I CCWWGG 1 cut(s) 172
Eco147I AGGCCT 2 cut(s) 110, 439
Eco24I GRGCYC 1 cut(s) 77
Eco31I GGTCTC 2 cut(s) 206, 495
Eco32I GATATC 1 cut(s) 229
Eco47III AGCGCT 1 cut(s) 66
Eco53kI GAGCTC 1 cut(s) 75
Eco81I CCTNAGG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 75
EcoRII CCWGG 2 cut(s) 215, 531
EcoRV GATATC 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 77
EgeI GGCGCC 1 cut(s) 136
EheI GGCGCC 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 1 cut(s) 267
FaiI YATR 4 cut(s) 192, 265, 293, 486
FatI CATG 1 cut(s) 263
Fnu4HI GCNGC 2 cut(s) 381, 561
FokI GGATG 1 cut(s) 532
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 2 cut(s) 381, 561
GlaI GCGC 3 cut(s) 66, 136, 143
GluI GCNGC 2 cut(s) 381, 561
HaeII RGCGCY 2 cut(s) 68, 138
HaeIII GGCC 3 cut(s) 110, 220, 439
HapII CCGG 3 cut(s) 121, 132, 555
HgaI GACGC 1 cut(s) 133
HhaI GCGC 3 cut(s) 67, 137, 144
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 1 cut(s) 267
Hin6I GCGC 3 cut(s) 65, 135, 142
HinP1I GCGC 3 cut(s) 65, 135, 142
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 2 cut(s) 7, 392
HpaII CCGG 3 cut(s) 121, 132, 555
HphI GGTGA 2 cut(s) 7, 88
Hpy166II GTNNAC 3 cut(s) 57, 344, 529
Hpy188I TCNGA 3 cut(s) 12, 79, 495
Hpy188III TCNNGA 4 cut(s) 31, 70, 224, 443
Hpy8I GTNNAC 3 cut(s) 57, 344, 529
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 341
HpyCH4IV ACGT 2 cut(s) 59, 81
HpyCH4V TGCA 4 cut(s) 281, 344, 431, 563
HpyF10VI GCNNNNNNNGC 1 cut(s) 141
HpyF3I CTNAG 2 cut(s) 222, 444
HpySE526I ACGT 2 cut(s) 59, 81
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 1 cut(s) 267
HspAI GCGC 3 cut(s) 65, 135, 142
KasI GGCGCC 1 cut(s) 134
Kzo9I GATC 3 cut(s) 12, 176, 495
LmnI GCTCC 1 cut(s) 80
Lsp1109I GCAGC 2 cut(s) 367, 547
LweI GCATC 1 cut(s) 268
MabI ACCWGGT 1 cut(s) 531
MaeII ACGT 2 cut(s) 59, 81
MaeIII GTNAC 1 cut(s) 94
MalI GATC 3 cut(s) 14, 178, 497
MboI GATC 3 cut(s) 12, 176, 495
MboII GAAGA 3 cut(s) 171, 223, 460
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 2 cut(s) 77, 346
MluCI AATT 3 cut(s) 308, 326, 452
Mly113I GGCGCC 1 cut(s) 135
MlyI GAGTC 1 cut(s) 401
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 7 cut(s) 38, 121, 125, 231, 307, 450, 462
MseI TTAA 2 cut(s) 465, 586
MspI CCGG 3 cut(s) 121, 132, 555
MspR9I CCNGG 2 cut(s) 217, 533
Mva1269I GAATGC 1 cut(s) 366
MvaI CCWGG 2 cut(s) 217, 533
MvnI CGCG 2 cut(s) 71, 144
MwoI GCNNNNNNNGC 1 cut(s) 141
NarI GGCGCC 1 cut(s) 135
NdeII GATC 3 cut(s) 12, 176, 495
NlaIII CATG 1 cut(s) 267
NlaIV GGNNCC 1 cut(s) 136
NmeAIII GCCGAG 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 94
NruI TCGCGA 1 cut(s) 71
PaqCI CACCTGC 1 cut(s) 272
PceI AGGCCT 2 cut(s) 110, 439
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 264
PkrI GCNGC 2 cut(s) 382, 562
PleI GAGTC 1 cut(s) 400
PluTI GGCGCC 1 cut(s) 138
PpsI GAGTC 1 cut(s) 400
Psp124BI GAGCTC 1 cut(s) 77
Psp6I CCWGG 2 cut(s) 215, 531
PspGI CCWGG 2 cut(s) 215, 531
PspN4I GGNNCC 1 cut(s) 136
PsuI RGATCY 1 cut(s) 176
RruI TCGCGA 1 cut(s) 71
RsaI GTAC 1 cut(s) 530
RsaNI GTAC 1 cut(s) 529
SacI GAGCTC 1 cut(s) 77
SaqAI TTAA 2 cut(s) 465, 586
SatI GCNGC 2 cut(s) 381, 561
Sau3AI GATC 3 cut(s) 12, 176, 495
SchI GAGTC 1 cut(s) 401
ScrFI CCNGG 2 cut(s) 217, 533
SduI GDGCHC 2 cut(s) 77, 346
SetI ASST 9 cut(s) 46, 62, 77, 84, 203, 218, 286, 352, 534
SexAI ACCWGGT 1 cut(s) 531
SfaNI GCATC 1 cut(s) 268
SfoI GGCGCC 1 cut(s) 136
SmlI CTYRAG 2 cut(s) 125, 202
SmoI CTYRAG 2 cut(s) 125, 202
Sse9I AATT 3 cut(s) 308, 326, 452
SseBI AGGCCT 2 cut(s) 110, 439
SsiI CCGC 2 cut(s) 38, 364
SspDI GGCGCC 1 cut(s) 134
SspI AATATT 1 cut(s) 411
SstI GAGCTC 1 cut(s) 77
StuI AGGCCT 2 cut(s) 110, 439
StyD4I CCNGG 2 cut(s) 215, 531
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 1 cut(s) 341
TaiI ACGT 2 cut(s) 62, 84
TaqI TCGA 2 cut(s) 159, 234
TasI AATT 3 cut(s) 308, 326, 452
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 2 cut(s) 465, 586
Tru9I TTAA 2 cut(s) 465, 586
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 2 cut(s) 380, 560
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 1 cut(s) 79
Van91I CCANNNNNTGG 1 cut(s) 264
VneI GTGCAC 1 cut(s) 342
XapI RAATTY 1 cut(s) 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.