MD17G1009400.v1.1

Translocon-associated protein subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0009086
Physical Location & Seq
Reverse (-)
592174 .. 594940
2767 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1009400.v1.1.491

Sequence Viewer

Length: 588 bp
ATGGCGATTCCGATCGGCAGCGCTCTCCTCGCGACGGTGGTAGCTCTCTTTCTATTCACGTCGGTGCTCGCGAGCTCCGACTTGCCCTTCATCGTCGCCCACAAGAAGGCCTCCCTCAACCGGCTCAAGTCCGGCGTCGAGCGCGTCCTTGTCTCCATCGACATCTACAACCAAGGATCTTCAACGGCTTATGATGTAAGCTTGAGTGATGATAGCTGGCCTCAAGATATCTTTGAGGTTGTCAGCGGTAACACTTCCAAGTCATGGGAAAGGCTTGATGCAGGTGCCATTATATCCCACTCTTTTGAATTGGAGGCCAAAGAAAAATTACTTTTCAATGGTGCACCAGCTCTTATTACATTCCGCATTCCCACAAAGGCTGCTTTACAGGAGTCTTATTCAACTCCAATATTTCCTTTGGATGTTCTGGCAGACAGGCCTCCTGAGAAGAAGTTTCAGTGGGTTAAGAGGTTTCTGGCAAAGTACGGGTCTCTGATCTCTGTTGTCTCCATCGTGGTTCTATTTGTGTACCTGGTTGCCAGTCCATCCAAATCCGGTGCTGCAAAAGGAAGCAAGAAGAAGCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
TRAP_beta

Protein Analysis

196

Amino Acids

21.22

Weight (kDa)

9.49

Isoelectric Point (pI)

28.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAP_beta PF05753 16 - 191 3.2e-55 Translocon-associated protein beta (TRAPB)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 272
Acc36I ACCTGC 1 cut(s) 272
AccB1I GGYRCC 1 cut(s) 284
AccB7I CCANNNNNTGG 1 cut(s) 264
AccII CGCG 3 cut(s) 32, 71, 144
AciI CCGC 2 cut(s) 246, 364
AclWI GGATC 1 cut(s) 184
AcyI GRCGYC 1 cut(s) 135
AfaI GTAC 2 cut(s) 485, 530
AfeI AGCGCT 1 cut(s) 22
AfiI CCNNNNNNNGG 4 cut(s) 34, 106, 120, 264
AgsI TTSAA 4 cut(s) 183, 308, 337, 402
AjiI CACGTC 1 cut(s) 60
AjnI CCWGG 1 cut(s) 531
AleI CACNNNNGTG 1 cut(s) 62
AluBI AGCT 5 cut(s) 44, 75, 201, 216, 350
AluI AGCT 5 cut(s) 44, 75, 201, 216, 350
Alw21I GWGCWC 3 cut(s) 69, 77, 346
Alw26I GTCTC 3 cut(s) 157, 495, 511
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 1 cut(s) 184
Aor51HI AGCGCT 1 cut(s) 22
AoxI GGCC 4 cut(s) 108, 218, 315, 437
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 3 cut(s) 18, 380, 560
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
AspLEI GCGC 2 cut(s) 23, 144
BaeGI GKGCMC 1 cut(s) 346
BanI GGYRCC 1 cut(s) 284
BanII GRGCYC 1 cut(s) 77
Bbv12I GWGCWC 3 cut(s) 69, 77, 346
BbvI GCAGC 3 cut(s) 30, 367, 547
BccI CCATC 3 cut(s) 164, 518, 553
BceAI ACGGC 1 cut(s) 201
BciT130I CCWGG 1 cut(s) 533
BcoDI GTCTC 3 cut(s) 157, 495, 511
BfoI RGCGCY 1 cut(s) 24
BfuAI ACCTGC 1 cut(s) 272
BisI GCNGC 3 cut(s) 19, 381, 561
BlsI GCNGC 3 cut(s) 20, 382, 562
Bme1390I CCNGG 1 cut(s) 533
BmgBI CACGTC 1 cut(s) 60
BmiI GGNNCC 1 cut(s) 286
BmrFI CCNGG 1 cut(s) 533
BmsI GCATC 1 cut(s) 268
BpuEI CTTGAG 3 cut(s) 110, 207, 223
BsaBI GATNNNNATC 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 135
BsaI GGTCTC 1 cut(s) 495
BsaJI CCNNGG 1 cut(s) 172
BsaWI WCCGGW 1 cut(s) 554
Bsc4I CCNNNNNNNGG 4 cut(s) 34, 106, 120, 264
Bse118I RCCGGY 1 cut(s) 120
Bse1I ACTGG 1 cut(s) 540
Bse8I GATNNNNATC 1 cut(s) 11
BseBI CCWGG 1 cut(s) 533
BseDI CCNNGG 1 cut(s) 172
BseGI GGATG 2 cut(s) 427, 545
BseJI GATNNNNATC 1 cut(s) 11
BseLI CCNNNNNNNGG 4 cut(s) 34, 106, 120, 264
BseMII CTCAG 1 cut(s) 435
BseNI ACTGG 1 cut(s) 540
BseRI GAGGAG 1 cut(s) 17
BseSI GKGCMC 1 cut(s) 346
BseXI GCAGC 3 cut(s) 30, 367, 547
Bsh1236I CGCG 3 cut(s) 32, 71, 144
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 4 cut(s) 110, 220, 317, 439
BshNI GGYRCC 1 cut(s) 284
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 3 cut(s) 69, 77, 346
BsiSI CCGG 3 cut(s) 121, 132, 555
BslI CCNNNNNNNGG 4 cut(s) 34, 106, 120, 264
BsmAI GTCTC 3 cut(s) 157, 495, 511
BsmI GAATGC 1 cut(s) 366
BsnI GGCC 4 cut(s) 110, 220, 317, 439
Bso31I GGTCTC 1 cut(s) 495
Bsp1286I GDGCHC 3 cut(s) 69, 77, 346
Bsp143I GATC 3 cut(s) 12, 176, 495
Bsp68I TCGCGA 2 cut(s) 32, 71
BspACI CCGC 2 cut(s) 246, 364
BspANI GGCC 4 cut(s) 110, 220, 317, 439
BspCNI CTCAG 1 cut(s) 436
BspFNI CGCG 3 cut(s) 32, 71, 144
BspLI GGNNCC 1 cut(s) 286
BspMI ACCTGC 1 cut(s) 272
BspPI GGATC 1 cut(s) 184
BspT107I GGYRCC 1 cut(s) 284
BspTNI GGTCTC 1 cut(s) 495
BsrFI RCCGGY 1 cut(s) 120
BsrI ACTGG 1 cut(s) 540
BssAI RCCGGY 1 cut(s) 120
BssECI CCNNGG 1 cut(s) 172
BssMI GATC 3 cut(s) 12, 176, 495
BssNI GRCGYC 1 cut(s) 135
BssT1I CCWWGG 1 cut(s) 172
Bst2UI CCWGG 1 cut(s) 533
Bst4CI ACNGT 1 cut(s) 37
BstACI GRCGYC 1 cut(s) 135
BstC8I GCNNGC 3 cut(s) 69, 73, 218
BstDEI CTNAG 1 cut(s) 444
BstF5I GGATG 2 cut(s) 427, 545
BstFNI CGCG 3 cut(s) 32, 71, 144
BstH2I RGCGCY 1 cut(s) 24
BstHHI GCGC 2 cut(s) 23, 144
BstKTI GATC 3 cut(s) 15, 179, 498
BstMAI GTCTC 3 cut(s) 157, 495, 511
BstMBI GATC 3 cut(s) 12, 176, 495
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 2 cut(s) 29, 141
BstNI CCWGG 1 cut(s) 533
BstSCI CCNGG 1 cut(s) 531
BstSLI GKGCMC 1 cut(s) 346
BstUI CGCG 3 cut(s) 32, 71, 144
BstV1I GCAGC 3 cut(s) 30, 367, 547
BstX2I RGATCY 1 cut(s) 176
BstYI RGATCY 1 cut(s) 176
BsuRI GGCC 4 cut(s) 110, 220, 317, 439
BtrI CACGTC 1 cut(s) 60
BtsCI GGATG 2 cut(s) 427, 545
BtsIMutI CAGTG 1 cut(s) 464
BtuMI TCGCGA 2 cut(s) 32, 71
BveI ACCTGC 1 cut(s) 272
Cac8I GCNNGC 3 cut(s) 69, 73, 218
CfoI GCGC 2 cut(s) 23, 144
Cfr10I RCCGGY 1 cut(s) 120
CseI GACGC 2 cut(s) 124, 133
CsiI ACCWGGT 1 cut(s) 531
Csp6I GTAC 2 cut(s) 484, 529
CviAII CATG 1 cut(s) 264
CviQI GTAC 2 cut(s) 484, 529
DdeI CTNAG 1 cut(s) 444
DpnI GATC 3 cut(s) 14, 178, 497
DpnII GATC 3 cut(s) 12, 176, 495
Ecl136II GAGCTC 1 cut(s) 75
Eco130I CCWWGG 1 cut(s) 172
Eco147I AGGCCT 2 cut(s) 110, 439
Eco24I GRGCYC 1 cut(s) 77
Eco31I GGTCTC 1 cut(s) 495
Eco32I GATATC 1 cut(s) 229
Eco47III AGCGCT 1 cut(s) 22
Eco53kI GAGCTC 1 cut(s) 75
EcoICRI GAGCTC 1 cut(s) 75
EcoRII CCWGG 1 cut(s) 531
EcoRV GATATC 1 cut(s) 229
EcoT14I CCWWGG 1 cut(s) 172
EcoT38I GRGCYC 1 cut(s) 77
ErhI CCWWGG 1 cut(s) 172
FaeI CATG 1 cut(s) 267
FaiI YATR 3 cut(s) 192, 265, 293
FatI CATG 1 cut(s) 263
Fnu4HI GCNGC 3 cut(s) 19, 381, 561
FokI GGATG 2 cut(s) 434, 532
FriOI GRGCYC 1 cut(s) 77
Fsp4HI GCNGC 3 cut(s) 19, 381, 561
GlaI GCGC 2 cut(s) 22, 143
GluI GCNGC 3 cut(s) 19, 381, 561
HaeII RGCGCY 1 cut(s) 24
HaeIII GGCC 4 cut(s) 110, 220, 317, 439
HapII CCGG 3 cut(s) 121, 132, 555
HgaI GACGC 2 cut(s) 124, 133
HhaI GCGC 2 cut(s) 23, 144
Hin1I GRCGYC 1 cut(s) 135
Hin1II CATG 1 cut(s) 267
Hin6I GCGC 2 cut(s) 21, 142
HinP1I GCGC 2 cut(s) 21, 142
HindIII AAGCTT 1 cut(s) 199
HinfI GANTC 2 cut(s) 7, 392
HpaII CCGG 3 cut(s) 121, 132, 555
Hpy166II GTNNAC 2 cut(s) 344, 529
Hpy188I TCNGA 3 cut(s) 12, 79, 495
Hpy188III TCNNGA 4 cut(s) 31, 70, 224, 443
Hpy8I GTNNAC 2 cut(s) 344, 529
Hpy99I CGWCG 4 cut(s) 37, 64, 98, 140
HpyAV CCTTC 2 cut(s) 97, 100
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 3 cut(s) 281, 344, 563
HpyF10VI GCNNNNNNNGC 2 cut(s) 29, 141
HpyF3I CTNAG 1 cut(s) 444
HpySE526I ACGT 1 cut(s) 59
Hsp92I GRCGYC 1 cut(s) 135
Hsp92II CATG 1 cut(s) 267
HspAI GCGC 2 cut(s) 21, 142
Kzo9I GATC 3 cut(s) 12, 176, 495
LmnI GCTCC 1 cut(s) 80
Lsp1109I GCAGC 3 cut(s) 30, 367, 547
LweI GCATC 1 cut(s) 268
MabI ACCWGGT 1 cut(s) 531
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 1 cut(s) 248
MalI GATC 3 cut(s) 14, 178, 497
MboI GATC 3 cut(s) 12, 176, 495
MboII GAAGA 2 cut(s) 171, 460
MflI RGATCY 1 cut(s) 176
MhlI GDGCHC 3 cut(s) 69, 77, 346
MluCI AATT 2 cut(s) 308, 326
MlyI GAGTC 1 cut(s) 401
MmeI TCCRAC 1 cut(s) 102
MnlI CCTC 8 cut(s) 38, 121, 125, 229, 231, 307, 450, 462
MseI TTAA 2 cut(s) 465, 586
MslI CAYNNNNRTG 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 246
MspI CCGG 3 cut(s) 121, 132, 555
MspR9I CCNGG 1 cut(s) 533
Mva1269I GAATGC 1 cut(s) 366
MvaI CCWGG 1 cut(s) 533
MvnI CGCG 3 cut(s) 32, 71, 144
MwoI GCNNNNNNNGC 2 cut(s) 29, 141
NdeII GATC 3 cut(s) 12, 176, 495
NlaIII CATG 1 cut(s) 267
NlaIV GGNNCC 1 cut(s) 286
NruI TCGCGA 2 cut(s) 32, 71
OliI CACNNNNGTG 1 cut(s) 62
PaqCI CACCTGC 1 cut(s) 272
PceI AGGCCT 2 cut(s) 110, 439
PcsI WCGNNNNNNNCGW 2 cut(s) 68, 75
PctI GAATGC 1 cut(s) 366
PfeI GAWTC 1 cut(s) 7
PflMI CCANNNNNTGG 1 cut(s) 264
PkrI GCNGC 3 cut(s) 20, 382, 562
Ple19I CGATCG 1 cut(s) 15
PleI GAGTC 1 cut(s) 400
PpsI GAGTC 1 cut(s) 400
Psp124BI GAGCTC 1 cut(s) 77
Psp6I CCWGG 1 cut(s) 531
PspGI CCWGG 1 cut(s) 531
PspN4I GGNNCC 1 cut(s) 286
PsuI RGATCY 1 cut(s) 176
PvuI CGATCG 1 cut(s) 15
RruI TCGCGA 2 cut(s) 32, 71
RsaI GTAC 2 cut(s) 485, 530
RsaNI GTAC 2 cut(s) 484, 529
RseI CAYNNNNRTG 1 cut(s) 62
SacI GAGCTC 1 cut(s) 77
SaqAI TTAA 2 cut(s) 465, 586
SatI GCNGC 3 cut(s) 19, 381, 561
Sau3AI GATC 3 cut(s) 12, 176, 495
SchI GAGTC 1 cut(s) 401
ScrFI CCNGG 1 cut(s) 533
SduI GDGCHC 3 cut(s) 69, 77, 346
SexAI ACCWGGT 1 cut(s) 531
SfaNI GCATC 1 cut(s) 268
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 3 cut(s) 125, 202, 222
SmoI CTYRAG 3 cut(s) 125, 202, 222
Sse9I AATT 2 cut(s) 308, 326
SseBI AGGCCT 2 cut(s) 110, 439
SsiI CCGC 2 cut(s) 246, 364
SspI AATATT 1 cut(s) 411
SstI GAGCTC 1 cut(s) 77
StuI AGGCCT 2 cut(s) 110, 439
StyD4I CCNGG 1 cut(s) 531
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 1 cut(s) 37
TaiI ACGT 1 cut(s) 62
TaqI TCGA 2 cut(s) 138, 159
TasI AATT 2 cut(s) 308, 326
TfiI GAWTC 1 cut(s) 7
Tru1I TTAA 2 cut(s) 465, 586
Tru9I TTAA 2 cut(s) 465, 586
TscAI CASTG 1 cut(s) 464
TseI GCWGC 3 cut(s) 18, 380, 560
TspDTI ATGAA 1 cut(s) 79
TspRI CASTG 1 cut(s) 464
Van91I CCANNNNNTGG 1 cut(s) 264
VneI GTGCAC 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.