AT5G14080

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015220
Physical Location & Seq
Reverse (-)
4543068 .. 4545416
2349 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14080.1

Sequence Viewer

Length: 1905 bp
ATGAGACCTGCGACGGAGCTTGCGGTGAGGATCGGCCGAGAGCTGCTAAAAGTTTCCGGAAGCTCTCGAGCTGCTCGGATATGGAGTCCATTGATTGAACAGAGTCTTCACGGTCTCGGTTTTCGTCACTCAATAAGCCCTTCGCTTGTGGCTCGAGTCATAGACCCGTTTCTGCTGAATCATCATTCTCTTGCTCTCGGTTTCTTCAATTGGGCTGCTCAGCAACCAGGTTACTCTCACGACTCCATATCTTACCACTCTATCTTCAAGTCTCTTTCGCTTTCTCGTCAGTTCTCCGCCATGGACGCTCTTTTCAAACAGGTTAAATCGAACAAAATCCTTCTCGATTCTTCCGTGTATCGCTCCCTCATTGATACGCTTGTGCTGGGTAGGAAAGCTCAGAGTGCGTTTTGGGTTTTAGAAGAAGCTTTTTCAACGGGTCAGGAGATTCATCCCGATGTATGCAATCGTCTTTTAGCTGGTTTAACTTCTGATGGATGTTATGATTATGCACAGAAACTGTTTGTTAAAATGCGTCATAAAGGTGTTTCGTTGAACACTCTTGGATTCGGTGTCTATATAGGATGGTTCTGTAGAAGTTCTGAAACGAATCAGCTCTTGAGATTGGTGGATGAGGTTAAAAAGGCTAATTTGAATATCAATGGCTCAATCATTGCACTGTTGATTCTCCATAGTCTTTGTAAGTGTTCACGAGAAATGGATGCTTTTTATATATTGGAAGAGCTAAGGAACATAGATTGCAAACCGGATTTTATGGCTTATAGGGTCATAGCTGAAGCGTTTGTAGTTACTGGGAATCTGTATGAGAGACAGGTTGTTTTAAAGAAGAAGAGAAAGCTCGGAGTAGCACCTAGGAGCAGCGATTACCGAGCTTTTATTCTGGATTTGATTTCAGCTAAGCGATTAACGGAGGCTAAGGAAGTGGCTGAGGTGATTGTGAGTGGAAAATTCCCTATGGATAATGATATCTTAGACGCGTTGATTGGATCTGTCTCTGCAGTTGATCCAGATTCTGCTGTTGAATTCTTAGTTTACATGGTCAGCACAGGGAAATTGCCTGCTATTCGGACTCTGAGCAAGTTAAGTAAGAATCTTTGCAGGCATGACAAGAGTGATCATCTGATAAAGGCTTATGAGCTTTTGTCAAGCAAAGGTTATTTCTCAGAACTTCAGAGTTACAGTCTGATGATATCGTTCTTGTGCAAGGCTGGGAGAGTCAGAGAAAGTTACACTGCTCTGCAAGAAATGAAGAAGGAAGGGCTTGCTCCAGACGTTTCACTCTATAACGCTCTCATTGAAGCTTGTTGCAAAGCGGAAATGATCCGTCCAGCAAAGAAACTGTGGGATGAGATGTTTGTGGAGGGCTGCAAAATGAATCTGACGACATATAATGTACTTATTAGGAAATTATCAGAGGAAGGCGAGGCAGAGGAGTCTTTAAGGCTGTTTGACAAGATGCTTGAGAGAGGGATAGAACCAGATGAAACAATCTATATGTCACTCATTGAAGGCCTATGCAAGGAGACAAAAATTGAAGCTGCTATGGAAGTTTTCAGAAAGTGCATGGAGAGGGATCATAAAACAGTTACAAGAAGAGTATTAAGCGAGTTTGTCTTAAATCTATGCAGCAACGGCCATTCTGGTGAGGCATCGCAGCTGCTGCGCGAGAGAGAACACTTAGAACACACAGGAGCACATGTCGTGTTGCTGAAATGTGTGGCGGATGCGAAAGAGGTTGAGATTGGAATTAGACATATGCAATGGATCAAAGAGGTATCGCCATCTCTTGTTCACACAATATCTTCTGACCTGTTGGCGTCTTTCTGTTCATCATCTGATCCTGATTCCATTCTTCCATTTATTCGAGCAATTGAGAACACATAA

Protein Analysis

634

Amino Acids

71.36

Weight (kDa)

7.83

Isoelectric Point (pI)

42.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 374 - 492 2e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 420 - 475 3.6e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 427 - 458 2.6e-07 PPR repeat
PPR_long PF17177 428 - 528 5.6e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 430 - 477 3.5e-13 PPR repeat family
PPR_2 PF13041 467 - 514 6.6e-11 PPR repeat family
PPR PF01535 469 - 498 3.6e-06 PPR repeat
PPR_1 PF12854 496 - 527 9.8e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015220)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G14080
fragaria_vesca FvH4_6g53850
malus_domestica MD09G1008000.v1.1
prunus_persica Prupe.3G308900_v2.0.a1 Prupe.3G308900_v2.0.a1
pyrus_communis pycom111g00640
rosa_chinensis RchiOBHm_Chr2g0175991
rosa_laevigata RLG00000022371
rosa_multiflora Rmu_sc0012513.1_g000006
rosa_roxburghii Rroxscaffold_2G00076880
rosa_rugosa Rorug02G0589200
rosa_samantha Rh2AG668400 Rh2BG679900 Rh2CG642800 Rh2DG693600
rosa_wichuraiana Rw2G054800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 16
AccII CGCG 2 cut(s) 998, 1686
AccIII TCCGGA 1 cut(s) 56
AciI CCGC 4 cut(s) 23, 297, 1334, 1742
AclWI GGATC 7 cut(s) 38, 1015, 1019, 1336, 1602, 1793, 1853
AcoI YGGCCR 2 cut(s) 34, 1654
AcsI RAATTY 2 cut(s) 968, 1043
AcuI CTGAAG 2 cut(s) 816, 1175
AcyI GRCGYC 1 cut(s) 1838
AfaI GTAC 1 cut(s) 1416
AfiI CCNNNNNNNGG 1 cut(s) 1540
AflIII ACRYGT 2 cut(s) 996, 1717
AjnI CCWGG 1 cut(s) 226
Alw21I GWGCWC 1 cut(s) 1717
Alw26I GTCTC 5 cut(s) 119, 276, 823, 1018, 1538
AlwI GGATC 7 cut(s) 38, 1015, 1019, 1336, 1602, 1793, 1853
AlwNI CAGNNNCTG 3 cut(s) 520, 1034, 1681
Ama87I CYCGRG 2 cut(s) 66, 153
Aor13HI TCCGGA 1 cut(s) 56
AoxI GGCC 3 cut(s) 34, 1531, 1654
ApoI RAATTY 2 cut(s) 968, 1043
AspA2I CCTAGG 1 cut(s) 872
AspLEI GCGC 1 cut(s) 1686
AsuHPI GGTGA 3 cut(s) 37, 964, 1676
AvaI CYCGRG 2 cut(s) 66, 153
AvrII CCTAGG 1 cut(s) 872
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BauI CACGAG 1 cut(s) 711
BbsI GAAGAC 1 cut(s) 98
Bbv12I GWGCWC 1 cut(s) 1717
BbvCI CCTCAGC 1 cut(s) 948
BccI CCATC 3 cut(s) 488, 579, 1810
BceAI ACGGC 1 cut(s) 1669
BciT130I CCWGG 1 cut(s) 228
BclI TGATCA 1 cut(s) 1135
BcoDI GTCTC 5 cut(s) 119, 276, 823, 1018, 1538
BfaI CTAG 1 cut(s) 873
BfmI CTRYAG 2 cut(s) 592, 1017
BfuAI ACCTGC 1 cut(s) 16
BlnI CCTAGG 1 cut(s) 872
BlpI GCTNAGC 2 cut(s) 219, 918
Bme1390I CCNGG 1 cut(s) 228
BmeT110I CYCGRG 2 cut(s) 66, 153
BmrFI CCNGG 1 cut(s) 228
BmrI ACTGGG 1 cut(s) 822
BmsI GCATC 4 cut(s) 712, 1467, 1679, 1735
BmuI ACTGGG 1 cut(s) 822
BpiI GAAGAC 1 cut(s) 98
BpmI CTGGAG 1 cut(s) 1272
Bpu10I CCTNAGC 3 cut(s) 746, 936, 948
Bpu1102I GCTNAGC 2 cut(s) 219, 918
BpuEI CTTGAG 2 cut(s) 640, 1502
BsaHI GRCGYC 1 cut(s) 1838
BsaI GGTCTC 1 cut(s) 119
BsaJI CCNNGG 2 cut(s) 300, 872
BsaWI WCCGGW 2 cut(s) 56, 766
BsaXI ACNNNNNCTCC 2 cut(s) 1704, 1734
Bsc4I CCNNNNNNNGG 1 cut(s) 1540
Bse1I ACTGG 1 cut(s) 817
Bse3DI GCAATG 2 cut(s) 672, 1787
BseAI TCCGGA 1 cut(s) 56
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 2 cut(s) 300, 872
BseGI GGATG 7 cut(s) 451, 503, 590, 637, 727, 1372, 1750
BseLI CCNNNNNNNGG 1 cut(s) 1540
BseMI GCAATG 2 cut(s) 672, 1787
BseMII CTCAG 5 cut(s) 233, 413, 939, 1085, 1197
BseNI ACTGG 1 cut(s) 817
BseRI GAGGAG 1 cut(s) 1466
BseX3I CGGCCG 1 cut(s) 34
BseYI CCCAGC 2 cut(s) 385, 1229
Bsh1236I CGCG 2 cut(s) 998, 1686
Bsh1285I CGRYCG 1 cut(s) 37
BshFI GGCC 3 cut(s) 36, 1533, 1656
BsiEI CGRYCG 1 cut(s) 37
BsiHKAI GWGCWC 1 cut(s) 1717
BsiHKCI CYCGRG 2 cut(s) 66, 153
BsiSI CCGG 2 cut(s) 57, 767
BslI CCNNNNNNNGG 1 cut(s) 1540
BsmAI GTCTC 5 cut(s) 119, 276, 823, 1018, 1538
BsnI GGCC 3 cut(s) 36, 1533, 1656
Bso31I GGTCTC 1 cut(s) 119
BsoBI CYCGRG 2 cut(s) 66, 153
Bsp1286I GDGCHC 1 cut(s) 1717
Bsp13I TCCGGA 1 cut(s) 56
Bsp143I GATC 8 cut(s) 30, 1007, 1024, 1135, 1341, 1594, 1785, 1858
Bsp1720I GCTNAGC 2 cut(s) 219, 918
Bsp19I CCATGG 1 cut(s) 300
BspACI CCGC 4 cut(s) 23, 297, 1334, 1742
BspANI GGCC 3 cut(s) 36, 1533, 1656
BspCNI CTCAG 5 cut(s) 232, 412, 940, 1086, 1196
BspEI TCCGGA 1 cut(s) 56
BspFNI CGCG 2 cut(s) 998, 1686
BspMAI CTGCAG 1 cut(s) 1021
BspMI ACCTGC 1 cut(s) 16
BspPI GGATC 7 cut(s) 38, 1015, 1019, 1336, 1602, 1793, 1853
BspQI GCTCTTC 1 cut(s) 735
BspTNI GGTCTC 1 cut(s) 119
BsrDI GCAATG 2 cut(s) 672, 1787
BsrI ACTGG 1 cut(s) 817
BssECI CCNNGG 2 cut(s) 300, 872
BssMI GATC 8 cut(s) 30, 1007, 1024, 1135, 1341, 1594, 1785, 1858
BssNI GRCGYC 1 cut(s) 1838
BssSI CACGAG 1 cut(s) 711
BssT1I CCWWGG 2 cut(s) 300, 872
Bst2BI CACGAG 1 cut(s) 711
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 6 cut(s) 113, 522, 681, 1202, 1362, 1606
Bst6I CTCTTC 3 cut(s) 735, 845, 1609
BstACI GRCGYC 1 cut(s) 1838
BstAPI GCANNNNNTGC 1 cut(s) 1681
BstC8I GCNNGC 4 cut(s) 21, 1080, 1121, 1284
BstDSI CCRYGG 1 cut(s) 300
BstENI CCTNNNNNAGG 1 cut(s) 1538
BstF5I GGATG 7 cut(s) 451, 503, 590, 637, 727, 1372, 1750
BstFNI CGCG 2 cut(s) 998, 1686
BstHHI GCGC 1 cut(s) 1686
BstKTI GATC 8 cut(s) 33, 1010, 1027, 1138, 1344, 1597, 1788, 1861
BstMAI GTCTC 5 cut(s) 119, 276, 823, 1018, 1538
BstMBI GATC 8 cut(s) 30, 1007, 1024, 1135, 1341, 1594, 1785, 1858
BstMCI CGRYCG 1 cut(s) 37
BstMWI GCNNNNNNNGC 4 cut(s) 305, 404, 1653, 1681
BstNI CCWGG 1 cut(s) 228
BstNSI RCATGY 1 cut(s) 1721
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 2 cut(s) 592, 1017
BstUI CGCG 2 cut(s) 998, 1686
BstV2I GAAGAC 1 cut(s) 98
BstX2I RGATCY 1 cut(s) 1007
BstYI RGATCY 1 cut(s) 1007
BstZI CGGCCG 1 cut(s) 34
BsuRI GGCC 3 cut(s) 36, 1533, 1656
BtgI CCRYGG 1 cut(s) 300
BtgZI GCGATG 1 cut(s) 1656
BtsCI GGATG 7 cut(s) 451, 503, 590, 637, 727, 1372, 1750
BtsI GCAGTG 1 cut(s) 1251
BtsIMutI CAGTG 2 cut(s) 677, 1251
BveI ACCTGC 1 cut(s) 16
Cac8I GCNNGC 4 cut(s) 21, 1080, 1121, 1284
CaiI CAGNNNCTG 3 cut(s) 520, 1034, 1681
CfoI GCGC 1 cut(s) 1686
CseI GACGC 4 cut(s) 314, 524, 1004, 1827
CsiI ACCWGGT 1 cut(s) 226
Csp6I GTAC 1 cut(s) 1415
CviAII CATG 5 cut(s) 301, 1057, 1124, 1585, 1718
CviQI GTAC 1 cut(s) 1415
DpnI GATC 8 cut(s) 32, 1009, 1026, 1137, 1343, 1596, 1787, 1860
DpnII GATC 8 cut(s) 30, 1007, 1024, 1135, 1341, 1594, 1785, 1858
DraI TTTAAA 1 cut(s) 843
EaeI YGGCCR 2 cut(s) 34, 1654
EagI CGGCCG 1 cut(s) 34
Eam1104I CTCTTC 3 cut(s) 735, 845, 1609
EarI CTCTTC 3 cut(s) 735, 845, 1609
EciI GGCGGA 2 cut(s) 286, 1757
EclXI CGGCCG 1 cut(s) 34
Eco130I CCWWGG 2 cut(s) 300, 872
Eco147I AGGCCT 1 cut(s) 1533
Eco31I GGTCTC 1 cut(s) 119
Eco32I GATATC 2 cut(s) 988, 1212
Eco52I CGGCCG 1 cut(s) 34
Eco57I CTGAAG 2 cut(s) 816, 1175
Eco88I CYCGRG 2 cut(s) 66, 153
EcoNI CCTNNNNNAGG 1 cut(s) 1538
EcoRI GAATTC 1 cut(s) 1043
EcoRII CCWGG 1 cut(s) 226
EcoRV GATATC 2 cut(s) 988, 1212
EcoT14I CCWWGG 2 cut(s) 300, 872
ErhI CCWWGG 2 cut(s) 300, 872
FaeI CATG 5 cut(s) 304, 1060, 1127, 1588, 1721
FatI CATG 5 cut(s) 300, 1056, 1123, 1584, 1717
FauNDI CATATG 1 cut(s) 1776
FbaI TGATCA 1 cut(s) 1135
FokI GGATG 7 cut(s) 438, 510, 597, 644, 734, 1379, 1757
FspBI CTAG 1 cut(s) 873
GlaI GCGC 1 cut(s) 1685
GsaI CCCAGC 2 cut(s) 389, 1233
GsuI CTGGAG 1 cut(s) 1272
HaeIII GGCC 3 cut(s) 36, 1533, 1656
HapII CCGG 2 cut(s) 57, 767
HgaI GACGC 4 cut(s) 314, 524, 1004, 1827
HhaI GCGC 1 cut(s) 1686
Hin1I GRCGYC 1 cut(s) 1838
Hin1II CATG 5 cut(s) 304, 1060, 1127, 1588, 1721
Hin6I GCGC 1 cut(s) 1684
HinP1I GCGC 1 cut(s) 1684
HindIII AAGCTT 2 cut(s) 426, 1320
HpaII CCGG 2 cut(s) 57, 767
HphI GGTGA 3 cut(s) 37, 964, 1676
Hpy166II GTNNAC 3 cut(s) 710, 1054, 1813
Hpy8I GTNNAC 3 cut(s) 710, 1054, 1813
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 6 cut(s) 150, 350, 1267, 1271, 1433, 1523
HpyCH4III ACNGT 6 cut(s) 113, 522, 681, 1202, 1362, 1606
HpyCH4IV ACGT 1 cut(s) 1293
HpyF10VI GCNNNNNNNGC 4 cut(s) 305, 404, 1653, 1681
HpySE526I ACGT 1 cut(s) 1293
Hsp92I GRCGYC 1 cut(s) 1838
Hsp92II CATG 5 cut(s) 304, 1060, 1127, 1588, 1721
HspAI GCGC 1 cut(s) 1684
Kpn2I TCCGGA 1 cut(s) 56
Ksp22I TGATCA 1 cut(s) 1135
Kzo9I GATC 8 cut(s) 30, 1007, 1024, 1135, 1341, 1594, 1785, 1858
LguI GCTCTTC 1 cut(s) 735
LmnI GCTCC 5 cut(s) 16, 368, 876, 1291, 1712
LweI GCATC 4 cut(s) 712, 1467, 1679, 1735
MabI ACCWGGT 1 cut(s) 226
MaeI CTAG 1 cut(s) 873
MaeII ACGT 1 cut(s) 1293
MaeIII GTNAC 7 cut(s) 125, 230, 808, 1196, 1247, 1518, 1606
MalI GATC 8 cut(s) 32, 1009, 1026, 1137, 1343, 1596, 1787, 1860
MboI GATC 8 cut(s) 30, 1007, 1024, 1135, 1341, 1594, 1785, 1858
MfeI CAATTG 2 cut(s) 208, 1890
MflI RGATCY 1 cut(s) 1007
MhlI GDGCHC 1 cut(s) 1717
MluCI AATT 9 cut(s) 208, 649, 968, 1043, 1073, 1427, 1551, 1767, 1890
MluI ACGCGT 1 cut(s) 996
MlyI GAGTC 7 cut(s) 94, 112, 165, 236, 1084, 1245, 1463
MroI TCCGGA 1 cut(s) 56
MslI CAYNNNNRTG 3 cut(s) 456, 543, 1662
MspA1I CMGCKG 1 cut(s) 1678
MspI CCGG 2 cut(s) 57, 767
MspR9I CCNGG 1 cut(s) 228
MunI CAATTG 2 cut(s) 208, 1890
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 2 cut(s) 998, 1686
MwoI GCNNNNNNNGC 4 cut(s) 305, 404, 1653, 1681
NcoI CCATGG 1 cut(s) 300
NdeI CATATG 1 cut(s) 1776
NdeII GATC 8 cut(s) 30, 1007, 1024, 1135, 1341, 1594, 1785, 1858
NlaIII CATG 5 cut(s) 304, 1060, 1127, 1588, 1721
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 2 cut(s) 125, 1518
NspI RCATGY 1 cut(s) 1721
PaeR7I CTCGAG 2 cut(s) 66, 153
PceI AGGCCT 1 cut(s) 1533
PciI ACATGT 1 cut(s) 1717
PciSI GCTCTTC 1 cut(s) 735
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PleI GAGTC 7 cut(s) 93, 111, 164, 236, 1084, 1244, 1462
PpsI GAGTC 7 cut(s) 93, 111, 164, 236, 1084, 1244, 1462
PscI ACATGT 1 cut(s) 1717
Psp6I CCWGG 1 cut(s) 226
PspFI CCCAGC 2 cut(s) 385, 1229
PspGI CCWGG 1 cut(s) 226
PspXI VCTCGAGB 1 cut(s) 153
PstI CTGCAG 1 cut(s) 1021
PstNI CAGNNNCTG 3 cut(s) 520, 1034, 1681
PsuI RGATCY 1 cut(s) 1007
PvuII CAGCTG 1 cut(s) 1678
RsaI GTAC 1 cut(s) 1416
RsaNI GTAC 1 cut(s) 1415
RseI CAYNNNNRTG 3 cut(s) 456, 543, 1662
SapI GCTCTTC 1 cut(s) 735
Sau3AI GATC 8 cut(s) 30, 1007, 1024, 1135, 1341, 1594, 1785, 1858
SchI GAGTC 7 cut(s) 94, 112, 165, 236, 1084, 1245, 1463
ScrFI CCNGG 1 cut(s) 228
SduI GDGCHC 1 cut(s) 1717
SexAI ACCWGGT 1 cut(s) 226
SfaNI GCATC 4 cut(s) 712, 1467, 1679, 1735
SfcI CTRYAG 2 cut(s) 592, 1017
Sfr274I CTCGAG 2 cut(s) 66, 153
SlaI CTCGAG 2 cut(s) 66, 153
SmiMI CAYNNNNRTG 3 cut(s) 456, 543, 1662
SmlI CTYRAG 4 cut(s) 66, 153, 619, 1481
SmoI CTYRAG 4 cut(s) 66, 153, 619, 1481
Sse9I AATT 9 cut(s) 208, 649, 968, 1043, 1073, 1427, 1551, 1767, 1890
SseBI AGGCCT 1 cut(s) 1533
SsiI CCGC 4 cut(s) 23, 297, 1334, 1742
SspMI CTAG 1 cut(s) 873
StuI AGGCCT 1 cut(s) 1533
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 2 cut(s) 300, 872
TaaI ACNGT 6 cut(s) 113, 522, 681, 1202, 1362, 1606
TaiI ACGT 1 cut(s) 1296
TaqI TCGA 5 cut(s) 67, 154, 329, 345, 1885
TasI AATT 9 cut(s) 208, 649, 968, 1043, 1073, 1427, 1551, 1767, 1890
TatI WGTACW 1 cut(s) 1414
TscAI CASTG 2 cut(s) 684, 1258
TseFI GTSAC 2 cut(s) 125, 1518
Tsp45I GTSAC 2 cut(s) 125, 1518
TspDTI ATGAA 5 cut(s) 440, 1283, 1409, 1518, 1839
TspGWI ACGGA 4 cut(s) 29, 343, 944, 1334
TspRI CASTG 2 cut(s) 684, 1258
XagI CCTNNNNNAGG 1 cut(s) 1538
XapI RAATTY 2 cut(s) 968, 1043
XceI RCATGY 1 cut(s) 1721
XhoI CTCGAG 2 cut(s) 66, 153
XmaJI CCTAGG 1 cut(s) 872
XspI CTAG 1 cut(s) 873
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.