AT5G16650

DNAJ heat shock N-terminal domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013956
Physical Location & Seq
Reverse (-)
5463177 .. 5465398
2222 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G16650.1

Sequence Viewer

Length: 387 bp
ATGGAAGACAATGATAAGTCTCCCCCTAAGGATTACTATAAGATTCTAGAAGTTGATTACGACGCAACTGAGGAATTGATCAGACTCAATTATCGGAAGCTTGCATTGAAGTGGCATCCTGATAAGCACAAAGGCGATAGTGCAGCTACAGAAAAATTTCAAGAGATTAATGAAGCTTATAATGTGCTGATGGACCCTGCTAAACGTTTTGAGTATGATTTCACTGGTATATATGAGATCCACAAATACACTTTACGGGAATATCTCGCCAGATTTAAGGGAATGATACTCACTTGCAATGGGCTCGGTATCAGCCAATCCTCATCACCATGGACACATCAATTGGCTGAGACCACCAAACAACAGACGAGCAAGGATACTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

128

Amino Acids

15.02

Weight (kDa)

5.8

Isoelectric Point (pI)

45.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 11 - 73 2.4e-25 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013956)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G16650 AT5G16650
fragaria_vesca FvH4_7g34190 FvH4_7g34190 FvH4_7g34190
prunus_persica Prupe.2G328700_v2.0.a1 Prupe.2G328700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0384541
rosa_laevigata RLG00000026033
rosa_roxburghii Rroxscaffold_4G00276610
rosa_rugosa Rorug01G0448800 Rorug01G0448900
rosa_samantha Rh1AG473900 Rh1BG432000 Rh1CG445100 Rh1DG464500
rosa_wichuraiana Rw1G039860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 180
AclI AACGTT 1 cut(s) 205
AclWI GGATC 1 cut(s) 232
AcsI RAATTY 1 cut(s) 155
AgsI TTSAA 2 cut(s) 109, 161
AluBI AGCT 3 cut(s) 100, 146, 176
AluI AGCT 3 cut(s) 100, 146, 176
Alw26I GTCTC 2 cut(s) 24, 344
AlwI GGATC 1 cut(s) 232
ApeKI GCWGC 1 cut(s) 143
ApoI RAATTY 1 cut(s) 155
AseI ATTAAT 1 cut(s) 168
Asp700I GAANNNNTTC 1 cut(s) 156
AspS9I GGNCC 1 cut(s) 193
AsuHPI GGTGA 1 cut(s) 318
AvaII GGWCC 1 cut(s) 193
AxyI CCTNAGG 1 cut(s) 27
BanII GRGCYC 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 12
BbvI GCAGC 1 cut(s) 155
BccI CCATC 1 cut(s) 184
BcgI CGANNNNNNTGC 2 cut(s) 286, 320
BciVI GTATCC 1 cut(s) 370
BclI TGATCA 1 cut(s) 78
BcoDI GTCTC 2 cut(s) 24, 344
BfaI CTAG 1 cut(s) 47
BfmI CTRYAG 1 cut(s) 147
BfuI GTATCC 1 cut(s) 370
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 1 cut(s) 193
BmiI GGNNCC 1 cut(s) 195
BmsI GCATC 1 cut(s) 124
BpiI GAAGAC 1 cut(s) 12
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 1 cut(s) 329
Bse1I ACTGG 1 cut(s) 229
Bse21I CCTNAGG 1 cut(s) 27
Bse3DI GCAATG 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 329
BseGI GGATG 1 cut(s) 115
BseMI GCAATG 1 cut(s) 304
BseMII CTCAG 2 cut(s) 60, 339
BseNI ACTGG 1 cut(s) 229
BseXI GCAGC 1 cut(s) 155
BsgI GTGCAG 1 cut(s) 162
BsmAI GTCTC 2 cut(s) 24, 344
Bso31I GGTCTC 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 306
Bsp143I GATC 2 cut(s) 78, 237
Bsp19I CCATGG 1 cut(s) 329
BspCNI CTCAG 2 cut(s) 61, 340
BspLI GGNNCC 1 cut(s) 195
BspPI GGATC 1 cut(s) 232
BspTNI GGTCTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 304
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 329
BssMI GATC 2 cut(s) 78, 237
BssT1I CCWWGG 1 cut(s) 329
Bst4CI ACNGT 1 cut(s) 382
BstC8I GCNNGC 1 cut(s) 102
BstDEI CTNAG 3 cut(s) 27, 69, 348
BstDSI CCRYGG 1 cut(s) 329
BstF5I GGATG 1 cut(s) 115
BstKTI GATC 2 cut(s) 81, 240
BstMAI GTCTC 2 cut(s) 24, 344
BstMBI GATC 2 cut(s) 78, 237
BstSFI CTRYAG 1 cut(s) 147
BstV1I GCAGC 1 cut(s) 155
BstV2I GAAGAC 1 cut(s) 12
BstX2I RGATCY 1 cut(s) 237
BstYI RGATCY 1 cut(s) 237
Bsu36I CCTNAGG 1 cut(s) 27
BsuI GTATCC 1 cut(s) 370
BtgI CCRYGG 1 cut(s) 329
BtsCI GGATG 1 cut(s) 115
BtsIMutI CAGTG 1 cut(s) 222
Cac8I GCNNGC 1 cut(s) 102
Cfr13I GGNCC 1 cut(s) 193
CseI GACGC 1 cut(s) 71
CviAII CATG 1 cut(s) 330
CviJI RGCY 6 cut(s) 100, 146, 176, 304, 315, 347
CviKI_1 RGCY 6 cut(s) 100, 146, 176, 304, 315, 347
DdeI CTNAG 3 cut(s) 27, 69, 348
DpnI GATC 2 cut(s) 80, 239
DpnII GATC 2 cut(s) 78, 237
Eco130I CCWWGG 1 cut(s) 329
Eco24I GRGCYC 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 1 cut(s) 193
Eco81I CCTNAGG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 329
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 329
FaeI CATG 1 cut(s) 333
FaiI YATR 8 cut(s) 39, 180, 216, 230, 232, 234, 331, 385
FatI CATG 1 cut(s) 329
FbaI TGATCA 1 cut(s) 78
Fnu4HI GCNGC 1 cut(s) 144
FokI GGATG 1 cut(s) 102
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 1 cut(s) 144
FspBI CTAG 1 cut(s) 47
GluI GCNGC 1 cut(s) 144
HgaI GACGC 1 cut(s) 71
Hin1II CATG 1 cut(s) 333
HindIII AAGCTT 2 cut(s) 98, 174
HinfI GANTC 2 cut(s) 43, 84
HphI GGTGA 1 cut(s) 318
Hpy188I TCNGA 2 cut(s) 83, 96
Hpy188III TCNNGA 3 cut(s) 47, 119, 161
Hpy99I CGWCG 1 cut(s) 65
HpyCH4III ACNGT 1 cut(s) 382
HpyCH4IV ACGT 1 cut(s) 205
HpyCH4V TGCA 3 cut(s) 104, 143, 297
HpyF3I CTNAG 3 cut(s) 27, 69, 348
HpySE526I ACGT 1 cut(s) 205
Hsp92II CATG 1 cut(s) 333
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 2 cut(s) 78, 237
LpnPI CCDG 4 cut(s) 132, 210, 210, 283
Lsp1109I GCAGC 1 cut(s) 155
LweI GCATC 1 cut(s) 124
MaeI CTAG 1 cut(s) 47
MaeII ACGT 1 cut(s) 205
MalI GATC 2 cut(s) 80, 239
MboI GATC 2 cut(s) 78, 237
MboII GAAGA 1 cut(s) 17
MfeI CAATTG 1 cut(s) 341
MflI RGATCY 1 cut(s) 237
MhlI GDGCHC 1 cut(s) 306
MluCI AATT 4 cut(s) 74, 88, 155, 341
MlyI GAGTC 1 cut(s) 78
MnlI CCTC 2 cut(s) 64, 331
MroXI GAANNNNTTC 1 cut(s) 156
MseI TTAA 2 cut(s) 168, 276
MslI CAYNNNNRTG 2 cut(s) 109, 328
MunI CAATTG 1 cut(s) 341
NcoI CCATGG 1 cut(s) 329
NdeII GATC 2 cut(s) 78, 237
NlaIII CATG 1 cut(s) 333
NlaIV GGNNCC 1 cut(s) 195
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 1 cut(s) 43
PkrI GCNGC 1 cut(s) 145
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
PshBI ATTAAT 1 cut(s) 168
PsiI TTATAA 1 cut(s) 180
Psp1406I AACGTT 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 195
PspPI GGNCC 1 cut(s) 193
PsuI RGATCY 1 cut(s) 237
RseI CAYNNNNRTG 2 cut(s) 109, 328
SaqAI TTAA 2 cut(s) 168, 276
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 2 cut(s) 78, 237
Sau96I GGNCC 1 cut(s) 193
SchI GAGTC 1 cut(s) 78
SduI GDGCHC 1 cut(s) 306
SetI ASST 4 cut(s) 102, 148, 178, 208
SfaNI GCATC 1 cut(s) 124
SfcI CTRYAG 1 cut(s) 147
SinI GGWCC 1 cut(s) 193
SmiMI CAYNNNNRTG 2 cut(s) 109, 328
Sse9I AATT 4 cut(s) 74, 88, 155, 341
SspMI CTAG 1 cut(s) 47
StyI CCWWGG 1 cut(s) 329
TaaI ACNGT 1 cut(s) 382
TaiI ACGT 1 cut(s) 208
TasI AATT 4 cut(s) 74, 88, 155, 341
TfiI GAWTC 1 cut(s) 43
Tru1I TTAA 2 cut(s) 168, 276
Tru9I TTAA 2 cut(s) 168, 276
TscAI CASTG 1 cut(s) 229
TseI GCWGC 1 cut(s) 143
TspDTI ATGAA 1 cut(s) 186
TspRI CASTG 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 193
VspI ATTAAT 1 cut(s) 168
XapI RAATTY 1 cut(s) 155
XbaI TCTAGA 1 cut(s) 46
XmnI GAANNNNTTC 1 cut(s) 156
XspI CTAG 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.